Next Article in Journal
A 3D QSAR Study of Betulinic Acid Derivatives as Anti-Tumor Agents Using Topomer CoMFA: Model Building Studies and Experimental Verification
Previous Article in Journal
Synthesis and Anti-HBV Activity of Novel 3′-N-phenylsulfonyl Docetaxel Analogs
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(9), 10213-10227;

Comparison of Two Methods, UHPLC-UV and UHPLC-MS/MS, for the Quantification of Polyphenols in Cider Apple Juices

Laboratoire SONAS, Université d'Angers, SFR QUASAV, Angers 49045, France
Institut de Recherche en Horticulture et Semences, UMR1345, INRA, Université d'Angers, AgroCampus-Ouest, SFR QUASAV, Angers 49045, France
Plateforme d'Ingénierie et Analyses Moléculaires, Université d'Angers, Angers 49045, France
Author to whom correspondence should be addressed.
Received: 11 July 2013 / Revised: 12 August 2013 / Accepted: 12 August 2013 / Published: 22 August 2013
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [408 KB, uploaded 18 June 2014]   |  


The aim of this study was to develop faster and more efficient phenotyping methods for in-depth genetic studies on cider apple progeny. The UHPLC chromatographic system was chosen to separate polyphenolic compounds, and quantifications were then simultaneously performed with a UV-PDA detector and an ESI-triple quadrupole mass analyzer (SRM mode). Both quantification methods were validated for 15 major compounds using two apple juice samples, on the basis of linearity, limits of detection and quantification, recovery and precision tests. The comparison between UV and SRM quantifications in 120 different samples of a cider apple progeny showed an excellent correlation for major compounds quantified with both methods. However, an overestimation was revealed for five compounds with the UV detector and the mass analyzer. Co-elution and matrix effects are discussed to explain this phenomenon. SRM methods should therefore be considered with restrictions in some cases for quantification measurements when several phenolic compounds are simultaneously quantified in complex matrices such as apple juices. For both methods, analyses were carried out over short periods of time while maintaining a high quality for the simultaneous quantification of phenolic compounds in apple juice. Each method is relevant for more in-depth genetic studies of the polyphenol content of apple juice. View Full-Text
Keywords: Malus x domestica; LC-UV; LC-MSn; phenolic compounds Malus x domestica; LC-UV; LC-MSn; phenolic compounds

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Verdu, C.F.; Gatto, J.; Freuze, I.; Richomme, P.; Laurens, F.; Guilet, D. Comparison of Two Methods, UHPLC-UV and UHPLC-MS/MS, for the Quantification of Polyphenols in Cider Apple Juices. Molecules 2013, 18, 10213-10227.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top