Next Article in Journal
Modulation of Xenobiotic Receptors by Steroids
Previous Article in Journal
TDAE Strategy for the Synthesis of 2,3-Diaryl N-Tosylaziridines
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(7), 7376-7388;

Ethanol Extract of Atractylodes macrocephala Protects Bone Loss by Inhibiting Osteoclast Differentiation

KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 27 May 2013 / Revised: 19 June 2013 / Accepted: 19 June 2013 / Published: 24 June 2013
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [2265 KB, uploaded 18 June 2014]   |  


The rhizome of Atractylodes macrocephala has been used mainly in Traditional Chinese Medicine for invigorating the functions of the stomach and spleen. In the present study, we investigated the inhibitory effect of the 70% ethanol extract of the rhizome of Atractylodes macrocephala (AMEE) on osteoclast differentiation. We found that AMEE inhibits osteoclast differentiation from its precursors induced by receptor activator of nuclear factor-κB ligand (RANKL), an essential cytokine required for osteoclast differentiation. AMEE attenuated RANKL-induced activation of NF-κB signaling pathway, subsequently inhibiting the induction of osteoclastogenic transcription factors, c-Fos and nuclear factor of activated T cells cytoplasmic 1. Consistent with the in vitro results, administration of AMEE protected RANKL-induced bone loss in mice. We also identified atractylenolide I and II as active constituents contributing to the anti-osteoclastogenic effect of AMEE. Taken together, our results demonstrate that AMEE has a protective effect on bone loss via inhibiting osteoclast differentiation and suggest that AMEE may be useful in preventing and treating various bone diseases associated with excessive bone resorption. View Full-Text
Keywords: Atractylodes macrocephala; osteoporosis; RANKL; osteoclast Atractylodes macrocephala; osteoporosis; RANKL; osteoclast

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Ha, H.; An, H.; Shim, K.-S.; Kim, T.; Lee, K.J.; Hwang, Y.-H.; Ma, J.Y. Ethanol Extract of Atractylodes macrocephala Protects Bone Loss by Inhibiting Osteoclast Differentiation. Molecules 2013, 18, 7376-7388.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top