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Article

Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes

by
Ming Zhao
,
Zhao-Feng Zhang
,
Ye Ding
,
Jun-Bo Wang
and
Yong Li
*
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(6), 7083-7092; https://doi.org/10.3390/molecules17067083
Submission received: 21 May 2012 / Revised: 30 May 2012 / Accepted: 6 June 2012 / Published: 11 June 2012
(This article belongs to the Section Natural Products Chemistry)
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Abstract

We investigated the effects of Astragalus polysaccharide (APS) on palmitate-induced insulin resistance in C2C12 skeletal muscle myotubes. Palmitate-reduced glucose uptake was restored by APS. APS prevented palmitate-induced C2C12 myotubes from impaired insulin signaling by inhibiting Ser307 phosphorylation of insulin receptor substrate-1 (IRS-1) and increasing Ser473 phosphorylation of Akt. Moreover, the increases in protein-tyrosine phosphatase-1B (PTP1B) protein level and NF-κB activation associated with palmitate treatment were also prevented by APS. However the treatment with APS didn’t change AMP-activated protein kinase (AMPK) activation in palmitate-induced myotubes. The results of the present study suggest that Astragalus polysaccharide inhibits palmitate-induced insulin resistance in C2C12 myotubes by inhibiting expression of PTP1B and regulating NF-κB but not AMPK pathway.
Keywords: insulin resistance; NF-κB; PTP1B; Astragalus polysaccharide insulin resistance; NF-κB; PTP1B; Astragalus polysaccharide

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MDPI and ACS Style

Zhao, M.; Zhang, Z.-F.; Ding, Y.; Wang, J.-B.; Li, Y. Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes. Molecules 2012, 17, 7083-7092. https://doi.org/10.3390/molecules17067083

AMA Style

Zhao M, Zhang Z-F, Ding Y, Wang J-B, Li Y. Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes. Molecules. 2012; 17(6):7083-7092. https://doi.org/10.3390/molecules17067083

Chicago/Turabian Style

Zhao, Ming, Zhao-Feng Zhang, Ye Ding, Jun-Bo Wang, and Yong Li. 2012. "Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes" Molecules 17, no. 6: 7083-7092. https://doi.org/10.3390/molecules17067083

APA Style

Zhao, M., Zhang, Z.-F., Ding, Y., Wang, J.-B., & Li, Y. (2012). Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes. Molecules, 17(6), 7083-7092. https://doi.org/10.3390/molecules17067083

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