Physicochemical Properties, in Vitro Antioxidant Activities and Inhibitory Potential against α-Glucosidase of Polysaccharides from Ampelopsis grossedentata Leaves and Stems
Abstract
:1. Introduction
2. Results
2.1. Physicochemical Properties of ALPS and ASPS
Sample | ALPS | ASPS |
---|---|---|
Yield (%) | 1.1 ± 0.1 a | 1.0 ± 0.1 a |
Protein (%) | 3.1 ± 0.3 a | 8.8 ± 0.4 b |
Neutral sugar (%) | 34.7 ± 2.4 b | 24.2 ± 1.7 b |
Uronic acid (%) | 27.5 ± 3.1 a | 40.0 ± 2.4 b |
Neutral sugar composition (mol ratio) | ||
L-rhamnose | 0.6 | 1.3 |
D-fucose | 0.2 | 0.2 |
L-arabinose | 2.5 | 2.2 |
D-xylose | 0.1 | 0.3 |
D-mannose | 0.8 | 3.7 |
D-glucose | 1.6 | 0.8 |
D-galactose | 4.2 | 1.6 |
2.2. Antioxidant Activities of ALPS and ASPS
2.2.1. DPPH Assay
2.2.2. ABTS Assay
2.2.3. FRAP Assay
2.2.4. FIC Assay
2.3. Inhibitory Activity on α-Glucosidase of ALPS and ASPS
3. Discussion
4. Experimental
4.1. Materials and Chemicals
4.2. Extraction and Isolation of ALPS and ASPS
4.3. Chemical Component Analysis
4.4. Monosaccharide Composition Analysis
4.5. Molecular Weight (MW) Determination
4.6. Infrared Spectral Analysis
4.7. Antioxidant Activities Determination
4.7.1. DPPH Assay
4.7.2. ABTS Assay
4.7.3. Ferric-Reducing Antioxidant Power (FRAP) Assay
4.7.4. Ferrous Ion Chelating (FIC) Assay
4.8. α-Glucosidase Inhibition Assay
4.9. Statistical Analysis
5. Conclusions
Conflict of Interest
References
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Wang, Y.; Bian, X.; Park, J.; Ying, L.; Qian, L.; Xu, P. Physicochemical Properties, in Vitro Antioxidant Activities and Inhibitory Potential against α-Glucosidase of Polysaccharides from Ampelopsis grossedentata Leaves and Stems. Molecules 2011, 16, 7762-7772. https://doi.org/10.3390/molecules16097762
Wang Y, Bian X, Park J, Ying L, Qian L, Xu P. Physicochemical Properties, in Vitro Antioxidant Activities and Inhibitory Potential against α-Glucosidase of Polysaccharides from Ampelopsis grossedentata Leaves and Stems. Molecules. 2011; 16(9):7762-7772. https://doi.org/10.3390/molecules16097762
Chicago/Turabian StyleWang, Yuefei, Xiaoying Bian, Jinhyouch Park, Le Ying, Lisheng Qian, and Ping Xu. 2011. "Physicochemical Properties, in Vitro Antioxidant Activities and Inhibitory Potential against α-Glucosidase of Polysaccharides from Ampelopsis grossedentata Leaves and Stems" Molecules 16, no. 9: 7762-7772. https://doi.org/10.3390/molecules16097762