Next Article in Journal
Cellular Protein Phosphatase 2A Regulates Cell Survival Mechanisms in Influenza A Virus Infection
Next Article in Special Issue
Mouse Breast Carcinoma Monocytic/Macrophagic Myeloid-Derived Suppressor Cell Infiltration as a Consequence of Endothelial Dysfunction in Shb-Deficient Endothelial Cells Increases Tumor Lung Metastasis
Previous Article in Journal
Phytoconstituents and Bioactivity of Plants of the Genus Spiraea L. (Rosaceae): A Review
Previous Article in Special Issue
Polycyclic Aromatic Hydrocarbons Detected in Processed Meats Cause Genetic Changes in Colorectal Cancers
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 20
10.3390/ijms222011160
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Protein Cargo of Salivary Small Extracellular Vesicles as Potential Functional Signature of Oral Squamous Cell Carcinoma

by
Simona Fontana
1,*,
Rodolfo Mauceri
2,3,4,
Maria Eugenia Novara
1,
Riccardo Alessandro
1,5 and
Giuseppina Campisi
2
1
Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90133 Palermo, Italy
2
Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy
3
Department of Biomedical and Dental Sciences, Morphological and Functional Images, University of Messina, 98124 Messina, Italy
4
Department of Dental Surgery, Faculty of Dental Surgery, University of Malta, 2090 Msida, Malta
5
Institute for Biomedical Research and Innovation (IRIB), National Research Council (CNR), 90146 Palermo, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(20), 11160; https://doi.org/10.3390/ijms222011160
Submission received: 23 September 2021 / Revised: 11 October 2021 / Accepted: 13 October 2021 / Published: 16 October 2021
(This article belongs to the Collection Feature Papers in Molecular Oncology)
Browse Figures
Versions Notes

Abstract

The early diagnosis of oral squamous cell carcinoma (OSCC) is still an investigative challenge. Saliva has been proposed as an ideal diagnostic medium for biomarker detection by mean of liquid biopsy technique. The aim of this pilot study was to apply proteomic and bioinformatic strategies to determine the potential use of saliva small extracellular vesicles (S/SEVs) as a potential tumor biomarker source. Among the twenty-three enrolled patients, 5 were free from diseases (OSCC_FREE), 6 were with OSCC without lymph node metastasis (OSCC_NLNM), and 12 were with OSCC and lymph node metastasis (OSCC_LNM). The S/SEVs from patients of each group were pooled and properly characterized before performing their quantitative proteome comparison based on the SWATH_MS (Sequential Window Acquisition of all Theoretical Mass Spectra) method. The analysis resulted in quantitative information for 365 proteins differentially characterizing the S/SEVs of analyzed clinical conditions. Bioinformatic analysis of the proteomic data highlighted that each S/SEV group was associated with a specific cluster of enriched functional network terms. Our results highlighted that protein cargo of salivary small extracellular vesicles defines a functional signature, thus having potential value as novel predict biomarkers for OSCC.
Keywords: saliva small extracellular vesicles; liquid biopsy; oral squamous cell carcinoma; protein profiling saliva small extracellular vesicles; liquid biopsy; oral squamous cell carcinoma; protein profiling

Share and Cite

MDPI and ACS Style

Fontana, S.; Mauceri, R.; Novara, M.E.; Alessandro, R.; Campisi, G. Protein Cargo of Salivary Small Extracellular Vesicles as Potential Functional Signature of Oral Squamous Cell Carcinoma. Int. J. Mol. Sci. 2021, 22, 11160. https://doi.org/10.3390/ijms222011160

AMA Style

Fontana S, Mauceri R, Novara ME, Alessandro R, Campisi G. Protein Cargo of Salivary Small Extracellular Vesicles as Potential Functional Signature of Oral Squamous Cell Carcinoma. International Journal of Molecular Sciences. 2021; 22(20):11160. https://doi.org/10.3390/ijms222011160

Chicago/Turabian Style

Fontana, Simona, Rodolfo Mauceri, Maria Eugenia Novara, Riccardo Alessandro, and Giuseppina Campisi. 2021. "Protein Cargo of Salivary Small Extracellular Vesicles as Potential Functional Signature of Oral Squamous Cell Carcinoma" International Journal of Molecular Sciences 22, no. 20: 11160. https://doi.org/10.3390/ijms222011160

APA Style

Fontana, S., Mauceri, R., Novara, M. E., Alessandro, R., & Campisi, G. (2021). Protein Cargo of Salivary Small Extracellular Vesicles as Potential Functional Signature of Oral Squamous Cell Carcinoma. International Journal of Molecular Sciences, 22(20), 11160. https://doi.org/10.3390/ijms222011160

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop