Search Results (8,493)

Background/Objectives: Whole-body cryotherapy (WBC), a brief exposure to extreme cold (−90 °C), has been proposed to modulate immune, metabolic, and stress-related pathways. This exploratory one-armed pilot study investigated the effects of an 18-session WBC protocol on immune markers, body composition, and perceived stress in healthy adults. Methods: Nineteen participants (mean age 52.9 ± 9.8 years) completed 18 WBC sessions over 9 weeks (3–6 min each), followed by a 9-week follow-up. Assessments were performed at baseline (M1), post-intervention (M2), and follow-up (M3). Primary outcomes included immune parameters (lymphocytes, granulocytes, cytokines, soluble ACE2), body composition (waist circumference, water compartments, lean mass), and perceived stress (Trier Inventory for Chronic Stress, TICS). Results: Waist circumference decreased from 83.8 ± 5.7 cm (M1) to 80.2 ± 4.2 cm (M2) (p = 0.001; M1 vs. M2; p = 0.004). Total body water (p = 0.008), lean body mass (p = 0.008), intracellular water (p = 0.005), and extracellular water (p = 0.021) also showed time-dependent effects. Immune modulation included increased lymphocytes (25.6 ± 7.1% to 29.3 ± 8.3%, p = 0.012) and decreased granulocytes (63.5 ± 6.8% to 58.7 ± 7.9%, p = 0.011) at M2. Anti-inflammatory IL-10 (virus-stimulated) rose markedly (33.5 ± 29.3 to 63.5 ± 50.5 pg/mL, p < 0.001), while IFN-γ (virus-stimulated) increased over time (p = 0.031). Soluble ACE2 decreased at follow-up (0.5 ± 0.7 to 0.3 ± 0.4 ng/mL, p = 0.029). Perceived stress improved in several TICS domains, including Work Overload (p = 0.009) and Pressure to Succeed (p = 0.018). Conclusions: This pilot study demonstrates that repeated WBC at −90 °C induces measurable changes in immune regulation, body composition, and perceived stress. These findings support the feasibility and potential physiological relevance of WBC and providing effect-size estimates for future randomized controlled trials. Full article

Drought stress severely limits wheat growth, development and yield. Endophytic fungi play a crucial role in plant growth and drought resistance. In agricultural production, they hold significant application potential as biocontrol agents capable of mitigating drought-induced damage. However, the mechanisms underlying changes in endophytic fungal community structure under drought stress remain unclear. Our study employed amplicon sequencing to investigate the structure of endophytic fungal communities in wheat roots under different water treatments, comparing structural and functional changes between different treatments. Results revealed that drought stress led to the greatest accumulation of relative abundance in the phylum Ascomycota (86.4%). At the genus level, Stachybotrys (increase 994.2%), Fusarium (increase 94.6%) and Aspergillus (increase 295.6%) showed the most significant increases in relative abundance. Co-occurrence network and Sankey diagram analysis revealed that wheat roots formed a drought-specific endophytic fungal community centered around Stachybotrys, Fusarium and Aspergillus, which indirectly enhanced crop drought tolerance. Our findings provide a theoretical foundation for future agricultural strategies to improve crop drought resistance through precise regulation of microbial communities. Full article

Electrocatalytic hydrogenation (ECH) represents an environmentally friendly pathway for converting 5-hydroxymethylfurfural (HMF) into the high-value chemical 2,5-bis(hydroxymethyl)furan (BHMF). However, its selectivity and Faradaic efficiency are often constrained by competitive hydrogen evolution at the cathode and insufficient supply of active hydrogen at the surface. To address this challenge, this study developed an Ir-decorated copper oxide nanowire catalyst (denoted as CuIr) featuring a hydrogen-rich adsorption (H_ads) surface. The incorporation of Ir significantly enhances the catalyst’s water dissociation capacity, creating abundant H_ads sources that selectively accelerate HMF hydrogenation while suppressing side reactions. Under a mild applied potential of −0.45 V vs. RHE and a current density of approximately −20 mA cm⁻², the optimal CuIr₄₀ catalyst achieved near-complete conversion of HMF (99%), a BHMF yield of 99%, and a high Faradaic efficiency of 97% within 120 min of electrolysis. Mechanistic studies reveal that this catalytic leap stems from the synergistic functional interaction between Cu and Ir sites in substrate activation and hydrogen supply. This work presents a novel strategy for designing efficient electrocatalysts for biomass hydrogenation by regulating surface H_ads concentration. Full article

Mangrove forests provide essential climate regulation and coastal protection, yet fine-scale quantification of carbon dynamics remains limited in the Sundarbans due to spatial heterogeneity and tidal influences. This study estimated canopy structural and photosynthetic dynamics from 2019 to 2023 by integrating 10 m spatial high-resolution remote sensing with a light use efficiency (LUE) modeling framework. Leaf Area Index (LAI) was retrieved at 10 m resolution using the PROSAIL radiative transfer model applied to Sentinel-2 data to characterize the canopy structure of the mangrove forest. LUE-based Gross Primary Productivity (GPP) was estimated using Sentinel-2 vegetation and water indices and MODIS fPAR with station observatory temperature data. Annual carbon uptake showed clear interannual variation, ranging from 1881 to 2862 g C m⁻² yr⁻¹ between 2019 and 2023. GPP estimates were strongly correlated with MODIS-GPP (R² = 0.86, p < 0.001), demonstrating the method’s reliability for monitoring mangrove carbon sequestration. LUE-based Solar-induced Chlorophyll Fluorescence (SIF) was derived at 10 m resolution and compared with TROPOMI-SIF observations to assess correspondence (R² = 0.88, p < 0.001) with photosynthetic activity. LAI, GPP and SIF exhibited pronounced seasonal and interannual variability on photosynthetic activity, with higher values during the monsoon growing season and lower values during dry periods. Mean NDVI declined from 2019 to 2023 and modeled annual carbon uptake ranged from approximately 43 to 65 Mt CO₂ eq, with lower sequestration in 2022–2023 associated with climatic stress. Strong correlations among LAI, NDVI, GPP, and SIF indicated consistent coupling between photosynthetic activity and carbon uptake in the mangrove ecosystem. These results provide a fine-scale assessment of mangrove carbon dynamics relevant to conservation and climate-mitigation planning in tropical regions. Full article

Mycelium-based composites (MBCs)—biomaterials made from fungal-inoculated substrates—are promising candidates to replace conventional rigid thermal insulation panels. However, many MBCs are made from hemp, a plant material that is quite difficult to source in many countries for regulation reasons, and mobilizes agricultural fields at the expense of food and feed crops. Meanwhile, many of our natural and urban ecosystems are subject to invasion by plants that are just burnt or even left in place, while they may be very good substrate for MBCs. This study investigated the comparative physical and thermal properties of MBCs derived from two distinct lignocellulosic feedstocks: hemp shives (a traditional material) and biomass from the highly invasive species Reynoutria japonica. Polyisocyanurate (PIR) was included as a synthetic benchmark. The MBCs produced from R. japonica demonstrated as low a thermal conductivity as the hemp MBCs in our internally developed method, but also as the PIR standard. However, they exhibited suboptimal physical characteristics: higher bulk density (166 vs. 128 kg/m³ for hemp) and significantly higher water absorption (7.5% vs. 3.5% volume uptake after 2 min). This suggest that they are a less viable alternative to hemp-based MBCs for heat insulation applications. Full article

Severe reservoir heterogeneity in offshore oilfields often leads to dominant flow channels, high water cut, and low sweep efficiency during long-term water flooding. In this study, an in situ self-assembled composite foam system combining soft polymer particles with a low-interfacial-tension foaming agent was developed for profile control and enhanced oil recovery (EOR) in offshore heterogeneous reservoirs. The self-assembly characteristics and physicochemical properties of different particle systems were evaluated to optimize the composite foam structure. Static and dynamic experiments were conducted to assess foam stability, plugging performance, injectivity behavior, and oil displacement efficiency. Results show that the optimized composite foam undergoes in situ self-assembly under reservoir conditions, forming a stable particle–foam structure that enhances selective plugging and mobility control. Core flooding experiments demonstrate that the system increases oil recovery by up to 27.2% across a wide permeability range. Field application further confirms its effectiveness in regulating interlayer water absorption, stabilizing injection pressure, and reducing water cut. These results indicate that the proposed in situ self-assembled composite foam is a promising technique for integrated profile control and enhanced oil recovery in offshore heterogeneous reservoirs. Full article

Sustained abiotic stress severely impairs fruit crop growth and development. As plants’ primary environmental sensing organ, fruit tree roots experience disrupted morphogenesis and physiological functions, reducing yield, lowering fruit quality, and threatening orchard ecosystem stability. Abiotic stress is diverse: water deficit from drought, extreme temperature fluctuations, and salinization-induced ion imbalance, heavy metal accumulation, or nutrient disorders. Its complexity requires synergistic and crosstalk regulation of multiple root-specific signaling modules and pathways in root stress perception and transduction. When responding to stress, roots activate hormone, reactive oxygen species (ROS), and calcium ion (Ca²⁺) signaling. These pathways mediate early stress recognition and regulate downstream gene expression and physiological metabolic reprogramming via transcription factors (TFs) and other regulators, determining stress tolerance and adaptability. Using typical abiotic stresses as models, this review outlines the composition, activation mechanisms, specificity, and synergistic effects of root-specific signaling modules/pathways, along with modern biotechnologies for decoding these modules and current research limitations, aiming to reveal the root signal network’s integration mode. Full article

Surface mulching and nitrogen (N) application are widely used to enhance crop yield and water productivity (WP). However, their combined effects remain unclear. Here, a three-year field experiment was conducted to comprehensively assess the effects of surface mulching (no mulching, B; straw mulching, S; and plastic film mulching, F) and N fertilization (no N application, N0; split application of urea, N1; 1:2 mixture of controlled-release urea and urea, N2) on maize growth, yield, and WP on the Loess Plateau. Application of nitrogen (N) significantly increased evapotranspiration (ET), grain yield, and WP by 4.58%, 176% (from 5215.43 kg ha⁻¹ in N0 to 14,548.21 kg ha⁻¹ in N2), and 166% (from 11.36 kg ha⁻¹ mm⁻¹ in N0 to 30.63 kg ha⁻¹ mm⁻¹ in N2), respectively. Compared with B and S, F increased ET during the pre-silking stage by 16.75% and 23.99%, respectively, and shortened the vegetative period of maize by 3–9 days but extended the duration from the milky stage (R3) to physiological maturity (R6) in the reproductive period by 5–13 days. F significantly increased yield and WP by 9.18% and 8.26% compared with S. Under F combined with N application, deep soil water (100–200 cm) consumption during R1–R3 increased by 15.75 mm and 13.15 mm compared with B and S, respectively. The combination of F and N2 achieved the highest yield (15,648.28 kg ha⁻¹) and WP (32.44 kg ha⁻¹ mm⁻¹) without causing detectable depletion of soil water within the 0–200 cm profile during the study period, providing an effective strategy for enhancing crop yield and improving water–fertilizer use efficiency in semi-arid regions. Full article

Mediterranean peri-urban forests play a crucial role in urban sustainability, yet their ecosystem services remain underexplored. This study quantifies and maps six regulating ecosystem services—carbon sequestration, air pollutant removal, surface runoff retention, precipitation interception, soil water regulation, and wildlife refuge—in a representative Mediterranean peri-urban forest, Monte de El Pardo (Spain). The analysis integrates cartographic and environmental data, biophysical modelling (i-Tree), and field surveys to provide a spatially explicit assessment. The results reveal that riparian formations and mixed stone pine–broadleaved woodlands provide the highest values across most services, while holm oak forests and dehesas contribute substantially due to their extensive coverage. Total annual carbon sequestration was estimated at 27,917,803 kg C yr⁻¹, equivalent to 102,329,511 kg CO₂e yr⁻¹. Hydrological regulation was also significant, with 94.5% of the area showing medium soil permeability and over half the territory presenting complex, multi-layered vegetation structure. Overall, Mediterranean peri-urban forests function as major carbon sinks, hydrological regulators, and biodiversity cores, reinforcing their importance as ecological and climatic stabilisers in metropolitan regions. Full article

Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder, often accompanied by low-grade inflammation, visceral hypersensitivity and gut microbiota dysbiosis. In this study, the therapeutic potential of Lactiplantibacillus plantarum LPPerfectus001 (L. plantarum 001) was investigated to alleviate IBS symptoms. Using an Lipopolysaccharides (LPS)-induced RAW264.7 macrophage model, L. plantarum 001 demonstrated significant anti-inflammatory properties by inhibiting Nitric Oxide production and downregulating pro-inflammatory cytokines. Furthermore, in a mouse model of IBS induced by Citrobacter rodentium infection and water avoidance stress, L. plantarum 001 intervention reduced fecal moisture, improved intestinal barrier integrity via up-regulating of ZO-1 and MUC2, and attenuated visceral hypersensitivity. Transcriptomic analysis combining with RT-qPCR revealed that L. plantarum 001 modulated the NF-κB signaling pathway and Th1/Th2 cell differentiation, reducing expression of key inflammatory genes. Additionally, 16S rRNA sequencing showed that L. plantarum 001 restored gut microbiota diversity, enriched beneficial butyrate-producing Odoribacter, and suppressed pro-inflammatory Pseudomonadota. These findings suggested that L. plantarum 001 alleviates IBS through multi-targeted mechanisms involving barrier repair, microbiota modulation, and anti-inflammatory signaling, highlighting its potential as a probiotic therapy for IBS. Full article

Wetlands in dryland regions are rapidly degrading under the combined effects of climate change and human regulation, yet long-term, seasonally resolved assessments of surface water extent (SWE) and its dynamics remain scarce. Here, we map and analyze seasonal surface water extent (SWE) over the period 2000–2024 in the Ile River Delta (IRD), south-eastern Kazakhstan, using Landsat TM/ETM+/OLI data within the Google Earth Engine (GEE) framework. We integrate multiple indices using the modified Normalized Difference Water Index (mNDWI), Automated Water Extraction Index (AWEI) variants, Water Index 2015 (WI2015), and Multi-Band Water Index (MBWI) with dynamic Otsu thresholding. The resulting index-wise binary water maps are merged via ensemble agreement (intersection, majority, union) to delineate three SWE regimes: stable (persists most of the time), periodic (appears regularly but not in every season), and ephemeral (appears only occasionally). Validation against Sentinel-2 imagery showed high accuracy F1-Score/Overall accuracy (F1/OA ≈ 0.85/85%), confirming our workflow to be robust. Hydroclimatic drivers were evaluated through modified Mann–Kendall (MMK) and Spearman’s (r) correlations between SWE, discharge (D), water level (WL), precipitation (P), and air temperature (AT), while a hybrid ensemble–occurrence framework was applied to identify degradation and transition patterns. Trend analysis revealed significant long–term declines, most pronounced during summer and fall. Discharge is predominantly controlled by stable spring SWE, while discharge and temperature jointly influence periodic SWE in summer–fall, with warming reducing the delta surface water. Ephemeral SWE responds episodically to flow pulses, whereas precipitation played a limited role in this semi–arid region. Spatially, area(s) of interest (AOI)-II/III (the main distributary system) support the most extensive yet dynamic wetlands. In contrast, AOI-I and AOI-IV host smaller, more constrained wetland mosaics. AOI-I shows persistence under steady low flows, while AOI-IV reflects a stressed system with sporadic high-water levels. Overall, the results highlight the dominant influence of flow regulation and distributary allocation on IRD hydrology and the need for ecologically timed releases, targeted restoration, and transboundary cooperation to sustain delta resilience. Full article

This study investigated the phytochemical composition and biological activity of Cymbopogon citratus microshoot cultures and evaluated their suitability for incorporation into a cosmetic formulation. Microshoot cultures were established on Murashige and Skoog media supplemented with plant growth regulators and served as a reproducible source of biomass. Methanolic and ethanolic extracts were analyzed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Chemical composition was characterized using LC-MS/MS analysis, which revealed the presence of phenolic acids and flavonoids, with p-coumaric, caffeic, and ferulic acids being among the most abundant detected constituents. In biological assays, the extracts inhibited murine tyrosinase in a concentration-dependent manner and exhibited antimicrobial activity against selected oral and skin-associated microorganisms, including Streptococcus mutans, Streptococcus pyogenes, and Staphylococcus epidermidis, as well as showing fungistatic and fungicidal effects against Candida albicans. Cytotoxicity analysis performed on HaCaT keratinocytes confirmed biocompatibility within the tested concentration range. To assess formulation suitability, the microshoot extract was incorporated into an oil-in-water (O/W) cream, which maintained stable pH, viscosity, and physical properties while preserving the antioxidant activity of the extract. Overall, these findings indicate that C. citratus microshoot cultures represent a reproducible source of bioactive metabolites with potential application in cosmetic formulations. Full article

Drought stress is a major abiotic factor limiting global crop productivity by disrupting cellular homeostasis, impairing photosynthesis, and restricting metabolic activity. Plant-associated microorganisms, including rhizobacteria, endophytes, and arbuscular mycorrhizal fungi, play key roles in enhancing drought resilience through molecular, biochemical, and physiological mechanisms. These beneficial microbes modulate phytohormone biosynthesis, enhance osmolyte accumulation, increase organic acid exudation, and activate ROS-scavenging antioxidant pathways. Microbe-mediated regulation of aquaporins, heat shock proteins, and root system architecture further improves water-use efficiency, hydraulic conductance, and stress acclimation. Advances in microbial genomics and systems biology have revealed the molecular drivers of plant–microbe synergism, enabling the development of tailored microbial consortia and next-generation bioinoculants. Complementarily, genetic and genome-guided modulation of drought-responsive regulatory hubs including transcription factors (DREB, NAC, MYB, bZIP), signal transducers (MAPKs, CDPKs), and protective proteins enhances adaptive plasticity under water deficit conditions. This review integrates current molecular insights into drought-induced perturbations in plants and highlights the convergence of microbial interventions and genome-guided strategies in reinforcing drought tolerance. Emphasizing mechanistic frameworks, scalable microbial technologies, and molecular breeding approaches, this work underscores their potential to improve crop resilience in increasingly water-limited environments. Full article

Green leaf volatiles (GLVs) are significant volatile signals that have been shown to protect plants against biotic and abiotic stresses, including insect herbivory and pathogen infections, as well as drought, cold, and heat stress. Since all these stresses are affected by climate change, GLVs provide an important target for research into their broad activities and their potential applications in agricultural settings. Therefore, to gain further insights into the protective properties of GLVs and their regulation under changing environmental conditions, we investigated whether climate-related changes alter the capacity to produce and the responsiveness to GLVs in Zea mayss, our model plant. Specifically, we studied the effects of limited nutrient supply, drought, and higher temperature. Neither significantly affected the capacity of plants to produce Z-3-hexenal as the first metabolite of the pathway, but elevated temperature increased E-2-hexenal production. We further identified changes in the effectiveness of plants to respond to GLVs under changing abiotic conditions by monitoring glucose levels and typical GLV-responsive genes covering metabolism, direct defense, indirect defense, and water stress. The results provide first evidence that plant responses to GLVs under defined environmentally challenging and stressful conditions are highly context-dependent and can vary substantially. Full article

As a vital ecological security barrier and climate regulator in northwestern China, the spatial patterns and evolving formation mechanisms of ecosystem services within the Qinghai Lake basin hold significant strategic value for ecological conservation and national park development in the region. This study selected land use data during 2000–2020, integrating the equivalent factor method, spatial correlation analysis, and the geodetector approach to systematically investigate the spatial heterogeneity characteristics of ESV in the Qinghai Lake basin and its corresponding driving mechanisms. The results indicate the following: (1) During the period 2000–2020, grassland consistently constituted the primary land cover category within the Qinghai Lake Basin, accounting for over 60% of the total area; water bodies (16.67%) and unused land (16.56%) represented the secondary land use categories. Over this twenty-year period, the total ESV exhibited a slight increasing trend, rising from USD 30.30 × 10⁸ to USD 30.75 × 10⁸, representing a growth of 0.31%. Regulating services constituted the primary component of ESV. The highest contribution to ESV originated from water bodies, with grassland ranking second. (2) ESV displayed a spatial arrangement marked by “high values in the lake center and low values in the surrounding areas” and “higher values in the southeast and lower values in the northwest.” Its spatial correlation exhibits a pronounced positive relationship. The number of units classified as high-high clusters (primarily water bodies at low elevations) and low-low clusters (mainly grasslands and unused land at high elevations) both increased over the study period, indicating a continuous intensification of ESV spatial agglomeration. (3) Results from the geographical detector reveal that both natural and anthropogenic factors collectively drive the spatial variation in ESV, with natural factors exhibiting stronger explanatory capacity. Among these, elevation and temperature are identified as the dominant drivers of ESV spatiotemporal differentiation. The combined effect of two interacting factors surpasses the influence exerted by any single factor in isolation. This research clarifies that the spatial distribution of ESV in the Qinghai Lake Basin, which features “high values in the lake center and low values in the surrounding areas” as well as “higher values in the southeast and lower values in the northwest,” is jointly shaped by the combined control of vertical zonality governed by topographic and climatic factors and the spatial differentiation of human activities. In low-altitude lakeshore zones, ESV rose as a consequence of water body expansion and the enforcement of ecological conservation measures, leading to the emergence of high-value clusters. In contrast, ESV improvement in high-elevation regions remained limited, constrained by fragile natural conditions and minimal human intervention. The insights derived from this research offer a scientific foundation for refining the “one core, four zones, one ring, multiple points” functional zoning framework of the Qinghai Lake National Park, as well as for developing tailored management approaches suited to distinct elevation-based regions. Full article