Search Results (9,320)

The flooded rice rhizosphere is a continuous reactive interface composed of sediment, porewater, root-surface oxic microdomains, and iron plaque, where redox processes and Fe cycling regulate Cd/As speciation, bioavailability, and plant accumulation. Engineered nanomaterials (ENMs) have shown potential for reducing Cd/As uptake in rice, but the coupled roles of microbial community responses, iron-plaque gating, and cross-interface elemental migration remain insufficiently integrated. This review synthesizes the current evidence on ENM transformation and partitioning at flooded rhizosphere microinterfaces, focusing on front-end speciation changes, root-surface retention, microbial functional regulation, and plant sequestration or transport. Correlative evidence suggests that rhizosphere microorganisms are associated with altered redox conditions, Fe cycling, As methylation potential, and metabolite secretion, which may influence Cd/As partitioning and cross-interface migration. However, direct causal validation of the complete ENM transformation–microbial response–Fe cycling–Cd/As flux–grain accumulation sequence within a single integrated system remains lacking. We further discuss how elevated CO₂, micro-/nanoplastics, Fe/DOM dynamics, and water management regimes may modify this framework, and we identify Sb as a theoretical boundary case because direct ENM–rice evidence remains limited. Finally, we highlight the need to integrate spatial tracing and imaging methods, including persistent luminescence tracing, LA-ICP-MS, NanoSIMS, and µ-XRF/µ-XANES, with metaomics to connect particle localization, microbial function, and contaminant fate. Full article

Global food production systems are increasingly challenged by population growth, climate change, water scarcity, and environmental degradation, necessitating the adoption of sustainable, resource-efficient food production strategies. Aquaponic systems integrate recirculating aquaculture with hydroponic crop cultivation, enabling nutrient recycling and improved water-use efficiency. Simultaneously, CRISPR/Cas9 genome-editing technology has emerged as a powerful tool for precise genetic improvement of economically important aquaculture traits. This review critically evaluates current progress in CRISPR/Cas9 applications in aquaculture, with emphasis on Nile tilapia (Oreochromis niloticus). Evidence from peer-reviewed studies indicates that targeted modification of genes associated with growth regulation, disease resistance, nutrient metabolism, feed efficiency, and stress tolerance can significantly enhance fish productivity and physiological resilience. Genes involved in hypoxia adaptation and nitrogen metabolism may further improve environmental performance in intensive recirculating systems by reducing ammonia accumulation and enhancing nutrient utilization. However, most genome-editing studies have been conducted under laboratory or conventional aquaculture conditions, with limited information available regarding the long-term performance, ecological interactions, microbial dynamics, and biosafety of genome-edited fish in aquaponic environments. Technical limitations including off-target effects, mosaicism, delivery efficiency, regulatory uncertainty, and public acceptance continue to constrain large-scale implementation. In the short term, CRISPR/Cas9 applications are likely to focus on practical trait enhancement under controlled aquaculture systems, whereas longer-term research may explore fish lines specifically optimized for nutrient cycling, environmental resilience, and integrated aquaponic sustainability. Overall, CRISPR/Cas9-mediated genome editing represents a promising but still emerging strategy for improving sustainable aquaculture and aquaponic food production systems. Full article

Gao-shan-hong-jing-tian (GSHJT) Oral Liquid is a phytochemical-rich preparation derived from Rhodiola, yet its anti-hypoxic active constituents and molecular mechanisms remain poorly understood. This study aimed to identify the key anti-hypoxic phytochemicals in GSHJT Oral Liquid and clarify their mechanisms of action to support its potential use in managing acute mountain sickness (AMS). We first established and validated an HPLC method for quality control, then comprehensively profiled the chemical composition using LC-MS. Network pharmacology and molecular docking were applied to predict the core anti-hypoxic components, candidate targets and signaling pathways. The primary bioactivity was further verified through an in vitro carbonic anhydrase (CA) inhibition assay. A total of 71 constituents were identified, with kaempferol and ellagic acid emerging as the primary anti-hypoxic phytochemicals. These compounds target seven core proteins (SRC, PIK3R1, ESR1, EGFR, PTK2, IGF1R, and LYN) to regulate vascular tone, inflammation, oxidative stress, blood–brain barrier integrity, and cell survival under hypoxic conditions. By modulating pathways such as HIF-1α, PI3K/AKT, FAK/PTK2, SRC, and IGF1R, these phytochemicals ultimately influence the onset and alleviation of AMS. Enzyme inhibition assays demonstrated that kaempferol and ellagic acid inhibited CA with IC₅₀ values of 34.05 μM and 119.1 μM, respectively. Molecular docking further revealed that both compounds suppressed CA activity through a combination of hydrogen bonding and hydrophobic interactions, consistent with a zinc-bound water-anchoring mechanism. This study elucidates the phytochemical basis and molecular mechanism responsible for the anti-hypoxic effects of GSHJT Oral Liquid, providing scientific support for its potential application as a natural, plant-derived intervention for preventing and alleviating acute mountain sickness, providing scientific support for its potential application and offering a reproducible paradigm for the rational development of other Rhodiola-based phytomedicines, though further in vivo validation is required to confirm the anti-hypoxic efficacy. Full article

Rice starch (RS) is widely consumed, but is usually rapidly digested, which may increase postprandial blood glucose levels. Therefore, regulating RS digestibility is important for development functional starch-based foods. In this study, sodium alginate (NaAlg) was incorporated into RS gels and subsequently crosslinked with Ca²⁺ to form a calcium alginate (CaAlg) network, and its effects on the physicochemical properties, digestion behavior, and physiological responses of RS gels were evaluated. Rheological measurement showed that the Ca²⁺-crosslinked alginate network increased the viscosity and viscoelastic moduli of RS gels. Low-field nuclear magnetic resonance analysis showed that the Ca²⁺-crosslinked alginate network reduced free water mobility. Structural characterization using Fourier-transform infrared spectroscopy, X-ray diffraction, and cold-field scanning electron microscopy shows that the Ca²⁺-crosslinked alginate network was associated with enhanced intermolecular interactions and a more continuous gel network, while all gelatinized samples exhibited predominantly amorphous structures. In vitro digestion experiments showed that the hydrolysis degree at 120 min decreased from 92.3% in RS to 85.6% in HCaAlg/RS. The rapidly digestible starch content significantly decreased from 72.4% to 68.4% (p < 0.05), while resistant starch significantly increased from 7.7% to 14.4% (p < 0.05). First-order kinetic fitting showed that C_∞ significantly decreased from 93.0% to 86.0%, and k significantly decreased from 0.027 to 0.013 min⁻¹ (p < 0.05). In vivo experiments showed that the Ca²⁺-crosslinked alginate/RS gels were associated with a lower postprandial glycemic response, with the incremental area under the curve significantly decreased from 747.2 to 591.7 mmol·min/L (p < 0.05), and the intestinal propulsion rate decreased from 89.6% to 75.3% (p < 0.05). These results suggest that Ca²⁺-crosslinked alginate network formation may modulate the structural properties, digestion behavior, and digestion-related physiological responses of RS gels, providing a basis for the development of starch-based functional foods with improved glycemic control. Full article

Honey maturity is increasingly discussed in relation to product integrity, fair trade, and the classification of immature honey production as a form of adulteration. This narrative critical review examines honey maturity using evidence from peer-reviewed microbiological, physicochemical, and metabolomic studies, combined with an analysis of international and European regulatory frameworks, including Codex Alimentarius CXS 12-1981, Council Directive 2001/110/EC, Regulation (EC) No 178/2002, and Regulation (EC) No 852/2004. Particular attention is given to the interpretation of osmophilic yeast counts, water activity ($a_w$), moisture content, comb cell capping, fermentation, and technological dehumidification. The reviewed evidence indicates that osmophilic yeasts are natural components of honey and that their presence, expressed as colony-forming units per gram (CFU/g), should not be treated as an independent non-compliance criterion in the absence of active fermentation. Existing honey standards define compositional and quality requirements, including moisture, hydroxymethylfurfural, enzymatic activity, and absence of fermentation or effervescence, but do not establish a honey-specific CFU/g limit for yeasts. On this basis, the review formulates a functional maturity assessment framework integrating $a_w$, moisture, enzymatic indicators, and metabolomic biomarkers. The proposed framework is presented as a conceptual model derived from the synthesis of the existing literature and requiring further multilaboratory validation prior to adoption in official control practice. This approach may improve proportionality in honey quality assessment and reduce the risk of misclassifying microbiologically stable honeys as immature or adulterated. Full article

Understanding trade-offs and synergies among ecosystem services (ESs) along environmental gradients is crucial for sustainable oasis management. This study investigated four key ESs—carbon storage (CS), habitat quality (HQ), water yield (WY), and soil conservation (SC)—in three typical oases along water–heat gradients in arid northwestern China. The InVEST model was used to quantify ESs in 1990, 2005, and 2022, and Pearson correlation, geographically weighted regression, K-means clustering, and random forest models were applied to analyze service relationships, ecosystem service bundles (ESBs), and driving factors. The results showed that CS and HQ maintained strong synergies, while the WY–SC relationship shifted from weak trade-offs under drier conditions to stronger synergies under more favorable water–heat conditions. Geographically weighted regression revealed spatial heterogeneity and directional asymmetry in ES relationships. Four ESB types were identified: ecologically fragile zones, ecological transition or buffer zones, agricultural production zones, and core ecological source zones. Driving-factor analysis indicated that vegetation-related services were mainly associated with land-cover structure and vegetation growth, whereas hydrological and erosion-related services were more closely linked to precipitation, potential evapotranspiration, temperature, and topography. These findings support differentiated oasis management through ecological restoration, development regulation, water-saving agriculture, and strict ecological protection. Full article

Sustainable land-use regulation in historical and cultural cities requires balancing heritage conservation, development demand, cropland retention, and urban–rural spatial restructuring. However, the stage-wise reorganization of urban–rural construction land under these coupled pressures remains insufficiently understood. Taking Jingzhou District, China, as a case study, this study uses land-use data from 2000, 2005, 2010, 2015, and 2020 and integrates stage-wise random-forest analysis, consistency-based interaction-network mining, and multi-scenario simulation within the intPLUS framework. Population, GDP, and areal-water distance layers were matched to the corresponding stage-terminal snapshots where applicable, whereas 2020 POI data were used as contemporary spatial-context proxies. From 2000 to 2020, urban industrial land (UIL) expanded from 16.63 to 46.42 km², increasing by approximately 179.1%, whereas rural settlements (RS) increased more moderately from 56.59 to 60.27 km², increasing by approximately 6.5%. The stage-wise RF and interaction-network results show that UIL and RS followed different spatial association structures, with stronger UIL self-reinforcement and stronger RS self-continuity in the later stage. Historical validation showed overall accuracy values of approximately 91% and Kappa values around 0.80, but FoM values remained relatively low, ranging from 0.098 to 0.176. Class-specific mapping accuracy was higher for RS (81.90–82.37%) than for UIL (55.20–66.93%), indicating a weaker performance in locating UIL change. Therefore, the 2035 simulations should be interpreted as parameter-conditioned regulatory comparisons rather than deterministic pixel-level forecasts. The scenario results indicate that the conservation-oriented limited growth was associated with the restricted UIL expansion and better cropland retention under the prescribed demand and constraint settings, while the RS reduction occurred only under explicit village-consolidation and construction-land quota reallocation assumptions. By distinguishing UIL and RS, this study provides differentiated regulation-oriented evidence for sustainable land-use governance in historical and cultural cities. Full article

The climate crisis, housing precarity, and the loss of everyday architectural heritage are converging challenges in Mediterranean cities. This article investigates the adaptive reuse of early twentieth-century adobe refugee dwellings in Nea Ionia and Kaisariani, neighborhoods of Attica, Greece, as an integrated social, environmental, and cultural strategy. Historical documentation, urban-morphological analysis, field observations, building survey data, material assessment, and design-based microclimatic analysis were combined to evaluate compatible restoration and bioclimatic upgrades as alternatives to demolition and conventional energy retrofit practices, with the main aim of preserving an important part of Greek history and architecture. The study develops a replicable qualitative assessment framework that identifies how existing adobe envelopes, compact layouts, courtyards, thresholds, vegetated pergolas, and low-water evaporative cooling may support low-carbon housing reuse. The results clarify the current preservation conditions and reuse potential of the selected case-study fragments, showing that adobe dwellings can preserve embodied material value, retain thermal mass and hygroscopic regulation, and support social housing when repaired with compatible, low-impact techniques. The article argues that the reuse of adobe refugee dwellings can function as a distributed urban strategy for housing provision, heritage continuity, and microclimatic adaptation. Its main contribution is a transferable analytical framework for assessing overlooked earthen housing stocks in dense Mediterranean contexts. The study argues that adaptive reuse can serve simultaneously as a means of social housing, a mechanism for optimizing the microclimate, and a means of preserving the tangible and intangible heritage of Greek adobe buildings that have been standing for over 100 years. This position extends circular construction debates by prioritizing non-demolition and direct reuse while preserving an important period of history. Full article

Achieving a favorable synergy of strength, ductility, and toughness is a critical challenge for expanding the engineering applications of titanium alloys. In this work, a medium-strength and high-toughness novel Ti-Al-Mo-V-Cr-Sn-Zr (named Ti62F) titanium alloy in the form of a Φ400 mm bar was adopted to systematically investigate the regulation behavior of double annealing on its microstructure and mechanical properties, and quantitative correlations between microstructural parameters and macroscopic properties were established. Increasing the cooling rate during the first annealing stage (air cooling, force air cooling and water quenching) significantly refined the secondary α (α_s) phase and reduced the volume fraction and size of the primary α (α_p) phase, leading to an increase in the ultimate tensile strength of the alloy from 1077 MPa to 1229 MPa. However, the impact-absorbed energy decreased from 51.5 J to 23.3 J. When the second annealing temperature was varied within the range of 625–675 °C, the ultimate tensile strength fluctuated slightly and the impact toughness increased moderately. Equiaxed α_p phase and relatively thick α_s can induce multiple crack deflections, prolong the crack propagation path and enhance energy absorption. Dislocations are mainly piled up at α/β phase boundaries, triggering void nucleation and growth, which dominate the ductility and toughness levels. Tensile twinning acts only as an auxiliary deformation mechanism and contributes limitedly to toughness. After heat treatment under the optimized schedule of 880 °C/2 h/AC + 650 °C/4 h/AC, the Ti62F alloy exhibits a superior strength–toughness balance compared with conventional medium-strength titanium alloys such as TA15, TC4, and TC4-DT. The findings can provide a heat treatment basis for microstructural regulation of large-size Ti62F bars and their engineering applications in aerospace structural components. Full article

The ecological consequences of hydraulic engineering on riverine environments have intensified the need for scientifically grounded ecological flow regimes. To ensure habitat suitability during critical fish spawning periods, this study developed habitat preference curves by correlating physiological parameters with key hydro-environmental drivers. A habitat suitability index (HSI) model was established using fuzzy logic, integrated with a genetic algorithm (GA) to simultaneously optimize fuzzy membership functions and inference rules. This model was applied to simulate the relationship between the weighted usable area (WUA) and discharge for various fish egg types in the reach downstream of the Fengman Dam, ultimately facilitating the determination of an optimized ecological pulse flow hydrograph. The results reveal distinct hydro-environmental preference variations among species. Specifically, drifting eggs require specific hatching cycles supported by higher flow magnitudes and velocities. Conversely, adhesive eggs experience a significant reduction in suitable habitat area under high-flow and high-velocity conditions. These findings suggest that reservoir water resource allocation must be tailored to the life-history requirements of target species to maximize spawning success. This study provides a robust scientific framework for eco-friendly reservoir scheduling and the conservation of regulated river ecosystems. Full article

Accurate forecasting of urban water demand is essential for scientific regulation and sustainable management of water resources, particularly in complex DMA (District Metered Area) environments. This study proposes an integrated regional water demand prediction framework that combines CEEMDAN decomposition with deep learning techniques. CEEMDAN is first applied to decompose the original water demand time series into multiple Intrinsic Mode Functions (IMFs), effectively extracting multi-scale features and mitigating non-stationarity and complexity. A hybrid Transformer-BiLSTM model is then constructed to capture global dependencies, nonlinear dynamics, and bidirectional temporal features. Experimental results demonstrate that the proposed CEEMDAN-Transformer-BiLSTM model significantly outperforms various benchmark models in terms of prediction accuracy, robustness, and generalization across different DMAs. This research provides a new perspective for modeling complex water resource time series and offers theoretical and practical support for optimizing urban water allocation and achieving sustainable management, while laying a foundation for future work involving external driving factors, enhanced model interpretability, and dynamic regulation mechanisms. Full article

Silver pomfret (Pampus argenteus), a highly valued marine fish, faces challenges in aquaculture due to its sensitivity to environmental conditions. Recirculating aquaculture system (RAS) is likely to become a primary indoor cultivation method for silver pomfret in the future, so studying hydrodynamic characteristics at varying flow rates in silver pomfret RAS is crucial and has far-reaching implications for both aquaculture practices and economic returns. This study investigated the effects of water flow rates (low: 400 L/h, moderate: 600 L/h, high: 800 L/h) on the growth performance, nutritional metabolism, and gut microbiota of silver pomfret over an 8-week period. Transcriptome and 16S rRNA sequencing revealed that the moderate-flow-rate group exhibited superior growth performance, with enhanced expression of genes related to protein synthesis (HYOU1, PDIA6, ITGA11) and redox regulation (GLUL, DUSP1, GST). Additionally, the moderate flow rate promoted gut microbial diversity and stability, with higher abundances of fermentation- and chemoheterotrophy-related functions, suggesting improved nutrient metabolism. In contrast, high flow rates induced anaerobic metabolism, leading to lactate accumulation and reduced growth. These findings demonstrate that a moderate flow rate of 600 L/h optimizes silver pomfret aquaculture by enhancing growth, metabolic efficiency, and microbial health, providing a foundation for sustainable large-scale farming practices. Full article

Endocrine-disrupting chemicals (EDCs) are a heterogeneous group of exogenous compounds capable of interfering with hormonal homeostasis and endocrine-regulated physiological processes. Their widespread occurrence in food, water, air, consumer products and industrial materials has raised increasing concern regarding their contribution to chronic disease burden. This review synthesizes current evidence on the exposure characteristics, molecular mechanisms, health effects, and prevention strategies related to major EDC classes, including bisphenol A and phthalates, dioxins and polychlorinated biphenyls, per- and polyfluoroalkyl substances, pesticides, and brominated flame retardants. Evidence indicates that EDCs may act through receptor-mediated signaling, altered hormone synthesis and metabolism, oxidative stress, mitochondrial dysfunction, immune modulation, and epigenetic mechanisms, with effects that may vary according to dose, timing, sex, age, and developmental susceptibility. Reported health outcomes include metabolic and cardiovascular disorders, reproductive dysfunction, hormone-dependent cancers, thyroid disruption, immune dysregulation, and adverse developmental effects. Although complete avoidance is unrealistic, exposure reduction and risk mitigation can be achieved through coordinated individual, clinical, environmental, and regulatory interventions. A life-course approach is essential to limit the health burden associated with endocrine disruption. Full article

This study investigates the synergistic regulation mechanism of water–heat–salt transport in the black soil of cold regions in Northeast China by combining field monitoring with HYDRUS-2D simulations. Four tillage treatments were evaluated: control group (CK), no-tillage with flat straw mulching (NM), ridge tillage with flat straw mulching (RM), and straw return with rotary tillage (RR). Monitoring data indicated that all straw incorporation treatments significantly improved soil moisture retention capacity. Compared with CK, soil water content under RM increased by 63.93% correspondingly; soil salinity in CK was 5.75–13.68% higher than that in straw-amended treatments. In addition, RM exerted a more prominent regulatory effect on soil temperature fluctuations relative to CK. Simulation results reveal that straw incorporation effectively reduces surface runoff, thereby substantially weakening the driving force for upward salt migration. Structural equation modeling (SEM) quantified path coefficients, revealing that straw incorporation optimizes the soil microenvironment. This integrated approach provides a mechanistic basis for black soil conservation in seasonally frozen regions, identifying RM as the optimal management practice to balance water retention and salt inhibition. Full article

Arsenic represents a ubiquitous element in the environment, characterized by high mobility, complex chemical speciation and a strong sensitivity to redox conditions and biological activity, with microbial processes play a central role in its biogeochemical cycling. The present review provides a comprehensive and integrative synthesis of arsenic biogeochemical cycling across terrestrial, freshwater and marine environments, in which chemical speciation is explicitly treated as the central unifying concept controlling arsenic mobility, transformation and bioavailability, linking geological, chemical and biological processes across environmental compartments. Natural processes regulating arsenic distribution are examined from mineralogical sources and soil–water interactions to biologically mediated transformations in aquatic and marine biotic compartments, largely driven by microbial activity, highlighting the contrast between inorganic arsenic dominance in abiotic reservoirs and the prevalence of organoarsenicals in tissues of living organisms. The review further explores arsenic behaviour under natural environmental alterations and in extreme or unconventional ecosystems, where redox constraints, sulphide chemistry or intense fluid–sediment exchanges lead to deviations from the baseline speciation patterns. Against this framework, anthropogenic perturbations are discussed through several documented case studies, illustrating how industrial releases, the long-term effects of mining activities, agricultural practices and the use of synthetic arsenical compounds may change arsenic pathways primarily by altering geochemical and biological controls rather than through a generalized increase in total arsenic content. Overall, the topics covered provide an integrated framework for interpreting arsenic dynamics across environmental systems, emphasizing the complex biogeochemical processes governing arsenic cycling. Full article