Search Results (1,913)

Since there are no available vaccines against some arboviruses, vector control is the most effective way to reduce their incidence. In this context, mechanical control is one of the most cost-effective ways to suppress Aedes populations, but it requires knowledge about vector breeding ecology in varied landscapes and climates. Here we investigated the infestation levels and container types used as larval habitats by Aedes aegypti and Aedes albopictus in an urban gradient of Vassouras, a countryside city in Rio de Janeiro. Larval surveys were conducted bimonthly from January 2017 to December 2018. Infestation was measured through the House (HI) and Breteau indexes (BI). Container types found with Aedes spp. immatures were correlated with temperature and rainfall and were compared between urbanization zones. The distribution of positive containers was mapped. The HI for Ae. aegypti increased during rainy seasons, but the HI and BI were always <1% for both mosquito vectors. More reservoirs were found harboring Ae. albopictus than Ae. aegypti, but in general their relative distribution into types was similar between species. On the other hand, the amount and distribution of containers into types varied across urbanization zones. Finally, the spatial distribution of larval habitats was similar between species, as well as often constant between seasons and study years. Full article

Driven by the Rural Revitalization Strategy, China has substantially increased its investment in rural infrastructure. Nevertheless, widespread issues such as underutilization and inadequate management persist. Recognizing rural infrastructure as a complex and interdependent system, this study applies complex network analysis to evaluate data from 98 counties, treating each county as an analytical unit and various infrastructure types as network nodes. A rural infrastructure interdependency network is constructed to examine the interdependencies among infrastructure and the overarching structural characteristics of the system. The analysis demonstrates that the rural infrastructure network exhibits pronounced modularity, with three distinct functional clusters: (1) electricity–water–broadband internet, (2) public service infrastructure, and (3) housing–environmental governance infrastructure. Furthermore, by employing a network dismantling approach that simulates facility management failures through the progressive removal of nodes, this study identifies paved roads and electricity supply stability as critical nodes within the rural infrastructure network. The failure of these infrastructures triggers systemic fragmentation and functional collapse, indicating their pivotal role in maintaining overall network integrity. These findings offer theoretical support for the optimization of infrastructure maintenance strategies, with the ultimate goal of enhancing the overall resilience and sustainable development capacity of rural infrastructure systems. Full article

A recent study challenges a fundamental principle of eukaryotic biology that each nucleus houses a complete genome. Two plant pathogenic fungi, Sclerotinia sclerotiorum and Botrytis cinerea, exhibit a segregated pattern of haploid chromosome distribution across two or more nuclei within each cell. The unequal distribution of the genome between nuclei suggests a coordinated system of internuclear recognition and regulation of cellular functions, a phenomenon previously associated with communication between nuclei of opposite mating type in both ascomycetes and basidiomycetes. Thus, the new study not only shatters expectations about genome biology but also opens new research avenues for understanding fungal adaptation and nuclear behavior. Full article

With the ongoing advancement of urbanization in China, a disparity has arisen between population demands and the allocation of community resources, resulting in a persistent increase in residential vacancy rates. The integration of service facilities into vacant residential spaces has enabled functional housing transformations. This study analyzes three typical types of communities in Xi’an to examine these transformations, identifying distinct types and patterns across five scenarios, which include social, health, leisure, cultural, and educational contexts. Through structured questionnaires and in-depth interviews, we collected data on residents’ life satisfaction and NIMBY (not in my backyard) perceptions. Applying a NIMBY index algorithm, we quantified characteristics and identified root causes. The results demonstrated that leisure scenarios most significantly affected satisfaction, while social scenarios showed the highest NIMBY index. Using an ordered logistic regression model, we determined key NIMBY factors influencing satisfaction across scenarios, revealing their differential impact mechanisms. Drawing on the findings, we investigated coordination mechanisms between the transformations of residential spaces and the needs of residents. Based on this analysis, the research objective was to explore how vacant housing can integrate service facilities while mitigating NIMBY effects and meeting resident needs. Proposed strategies include hierarchical facility allocation, NIMBY mitigation measures, and spatial planning optimization, ultimately adapting to diverse lifestyles and housing demands. Full article

Campylobacter is the most common cause of bacterial foodborne illness in the EU, primarily linked to poultry. To better understand its transmission dynamics, we applied whole-genome sequencing (WGS) to Campylobacter isolates collected at slaughterhouses over a two-year period from broilers originating from 26 Danish farms. The samples included cloacal swabs and boot sock samples from broiler houses and surrounding farm environments. We identified 150 distinct cgMLST types among 883 isolates. While most cgMLST types were flock-specific, some persisted across production cycles or appeared at different farms, indicating entrenched contamination or potential common-source introductions. Notably, 39% of broiler-associated cgMLST types overlapped with human clinical isolates from the same period, with the strongest overlap among persistent and cross-farm types, particularly in conventional production systems. Our findings underscore the need for strengthened biosecurity, targeted surveillance of high-risk genotypes, and real-time WGS integration to mitigate the burden of human Campylobacteriosis. This study supports a One Health approach to managing zoonotic risk in poultry production. Full article

Background/Objectives: The non-small-cell lung cancer (NSCLC) therapeutic landscape has undergone a profound transformation with the introduction of multiple personalized treatment options. Mutations in ERBB2 (HER2) have recently emerged as promising novel targets for the treatment of non-squamous NSCLC (nsNSCLC). Accurate, rapid, and efficient molecular profiling is crucial for identifying patients who may benefit from targeted therapies, including HER2-directed agents. Materials and Methods: Here, we aimed to retrospectively assess the performance of the Oncomine™ Precision Assay* (OPA) in combination with the Ion Torrent Genexus™ Integrated Sequencer* (Thermo Fisher Scientific. Waltham, MA, USA) for detecting ERBB2 mutations in nsNSCLC. A total of 108 archived nsNSCLC samples, consisting of biopsies, resections, and cytological specimens, were used to assess concordance with in-house-validated orthogonal tests. Results: The OPA showed high sensitivity and specificity with an overall accuracy of 100% for single-nucleotide variants (SNVs) and insertions and deletions (Indels). SNVs and Indels with allele frequencies as low as 5% were correctly identified across samples with a tumor cell content ranging from 5% to 95%. Additionally, the assay demonstrated high reproducibility across the six participating laboratories. The turnaround time of the OPA was notably shorter compared to traditional orthogonal methods, facilitating rapid molecular report generation. Conclusions: The OPA in combination with the Ion Torrent Genexus™ System allows for highly sensitive and specific detection of relevant ERBB2 mutations. The assay’s streamlined workflow, coupled with its automated data analysis pipeline, enables a fast turnaround time for testing across a range of sample types. This includes samples with reduced tumor cell content and limited available input. This study demonstrates the future potential of using this assay in a clinical setting. Full article

Space layout design has been recognized as a key technical challenge in achieving low-energy and low-carbon rural houses. Adjustment of room location can influence building energy performance and is subject to both meteorological and design parameters. To elucidate the impact of these parameters on the energy saving potential of room relocation (ESR), this study investigated rural houses in Northwest China using dynamic simulations to compare the relative energy saving rates (RES) associated with three types of single heated room location changes: from the west side to the middle (WM), from the east side to the middle (EM), and from the west side to the east side (WE). Simulations were conducted across different climate regions (Lhasa, Xi’an, Tuotuohe, and Altay) and design parameters, including exterior wall U-value, building orientation (BO), building height (BH), and window-to-wall ratio (WWR). Additionally, the maximum differences in energy consumption (MD) among six layouts with multiple heated rooms were assessed. The results demonstrated that ESR varied significantly with room relocation. The ranges of RES_WM, RES_EM, and RES_WE were −7.89% to 13.20%, −7.82% to 10.25%, and −2.29% to 3.36%, respectively. The MD values ranged from 2.42% to 15.01%. For single heated rooms, including direct normal irradiance (I_dn), the difference between east and west solar-air temperature (△T_sa), outdoor dry bulb temperature (T_e), exterior wall heat transfer coefficient (U), and WWR significantly influenced RES_WM and RES_EM. The ranking of the factor contributions was U > △T_sa > I_dn > T_e > WWR for RES_WM and U > I_dn > △T_sa > T_e > WWR for RES_EM. In the case of RES_WE, I_dn, △T_sa, T_e, exterior wall U value, and BO had significant effects, ranking I_dn > △T_sa > T_e > BO > U. For MD, the key influencing factors were I_dn, △T_sa, T_e, exterior wall U value, and WWR, which were ranked as I_dn > △T_sa > U > T_e > WWR. The effects of design parameters on ESR varied under different climatic conditions. In high-temperature regions, the exterior wall U-value had a stronger influence on the ESR of WE. In regions with larger |△T_sa|, BO exerted a more pronounced effect on the ESR of WE. In regions characterized by high temperatures and radiation, WWR and BH significantly influenced the ESR of WM and EM. Similarly, in these regions, WWR and BH exhibited a greater impact on MD. Finally, among the meteorological parameters, I_dn and △T_sa were significantly correlated with ESR (p < 0.01). These findings provide a valuable reference for the energy-efficient layout design of rural houses in Northwest China and cold regions and support the future development of intelligent and automated rural residential spatial layout design. Full article

Hybrid ventilation is indicated as one of the effective methods of maintaining thermal comfort and indoor air quality and reducing energy consumption in buildings. It assumes the capacity to switch between natural and mechanical ventilation, allowing the most efficient use of the outdoor air potential. This article aims to quantify the impact of changing ventilation system, from natural to hybrid, on indoor air parameters and air change rates in a bedroom of a single-family house. The distinct aspects of this study include longitudinal measurement over three years, natural ventilation substituted by hybrid ventilation halfway into the monitoring period, and unaltered building and user characteristics. The analysis is based on measurements of temperature, relative humidity, CO2 concentration, and window opening for three seven-month measurement periods from September 1 to March 31. The measurements are complemented by in-depth user feedback and an audit of the building structure and installed HVAC systems. A clear correlation was observed between the values of relative humidity and carbon dioxide concentration and the type of ventilation strategy. A significant influence of residents’ behavior on the achieved indoor air parameters was observed. Full article

The development of industry and technology, despite making everyday life easier, generates large amounts of various wastes that negatively affect the environment. Unexpected leaks of substances such as oils, petroleum substances, and chemicals also contribute to the degradation of aquatic and terrestrial ecosystems. Long-term effects of environmental pollution require the development of advanced materials and technologies to collect and neutralize pollutants. Sorbents obtained from waste, including banana peels, coconut fibers, and polyurethane foams from recycling the thermal housing of refrigeration devices, allow a reduction in the amount of generated waste and the development of appropriate sorbents. This work focuses on comparing the sorption and neutralization properties of these materials for two types of oil, machine and diesel, and the possibility of using them in rescue and firefighting operations conducted by firefighters. The results obtained indicate that the viscose–cellulose sorbent and the polyurethane foam sorbent are characterized by better performance parameters than sorbents from coffee grounds or coconut fibers. The best parameters were obtained after the first 10 min of the sorbent–contaminant reaction, whereas in the case of contamination with machine oil, the absorption capacity was better than for diesel oil for each sorbent subjected to analysis. Full article

A new generation of compact radon detectors with high sensitivity and fine spatial resolution (1–2 cm scale) was used to investigate indoor radon distribution and identify potential entry pathways. Solid-state nuclear track detectors (Kodak-Pathe LR-115 type II, Dosirad, France), combined with activated carbon fabric (ACC-5092-10), enabled sensitive, spatially resolved radon measurements. Two case studies were conducted: Case 1 involves a room with elevated radon levels suspected to originate from the floor. Case 2 involves a house with persistently high indoor radon concentrations despite active basement ventilation. In the first case, radon emission from the floor was found to be highly inhomogeneous, with concentrations varying by more than a factor of four. In the second, unexpectedly high radon levels were detected at electrical switches and outlets on walls in the living space, suggesting radon transport through wall voids and entry via non-hermetic electrical fittings. These novel detectors facilitate fine-scale mapping of indoor radon concentrations, revealing ingress routes that were previously undetectable. Their use can significantly enhance radon diagnostics and support the development of more effective mitigation strategies. Full article

Millions of puppies are welcomed into the homes of families around the world each year. However, understanding the ways in which puppies are bred and raised by their breeders, as well as the perspectives and perceptions underpinning these practices, is still in its infancy. The current study administered an online survey to 200 Australian dog breeders to investigate their breeding program characteristics, breeding dog selection, understanding of the importance of early experiences in puppyhood, and the extent and diversity of their puppy rearing and socialisation practices. Results indicated that breeders were motivated by breed improvement and producing dogs for themselves rather than providing companion dogs, despite most of their puppies being placed in companionship roles. The participating breeders also acknowledged the important role they play in shaping puppies’ behaviour and temperament, which was reflected in both their breeding dog selection and in their rearing and socialisation practices. The majority of breeders housed their litters within their residence for the initial weeks of life but the socialisation experiences they provided were variable in type and frequency. Longer-term breeders and those with larger, more intensive programs reported providing human-focused socialisation experiences less frequently, though the correlational nature of these findings require cautious interpretation. Whilst future research should endeavor to explore these results more comprehensively among a more diverse sample, these findings provide valuable insight into the breeding, rearing, and socialisation process undertaken by dog breeders in Australia. Full article

Tuberculosis (TB) remains a leading cause of death in Bangladesh, disproportionately affecting low socio-economic status (SES) populations. This review, guided by the WHO Social Determinants of Health framework and Rockefeller-Lancet Planetary Health Report, examined how social, economic, and ecological factors link SES to TB burden. The first literature search identified 28 articles focused on SES-TB relationships in Bangladesh. A second search through snowballing and conceptual mapping yielded 55 more papers of diverse source types and disciplines. Low-SES groups face elevated TB risk due to smoking, biomass fuel use, malnutrition, limited education, stigma, financial barriers, and hazardous housing or workplaces. These factors delay care-seeking, worsen outcomes, and fuel transmission, especially among women. High-SES groups more often face comorbidities like diabetes, which increase TB risk. Broader contextual drivers include urbanisation, weak labour protections, cultural norms, and poor governance. Recommendations include housing and labour reform, gender parity in education, and integrating private providers into TB programmes. These align with the WHO End TB Strategy, UN SDGs and Planetary Health Quadruple Aims, which expand the traditional Triple Aim for health system design by integrating environmental sustainability alongside improved patient outcomes, population health, and cost efficiency. Future research should explore trust in frontline workers, reasons for consulting informal carers, links between makeshift housing and TB, and integrating ecological determinants into existing frameworks. Full article

Background: Environmental enrichment is a promising strategy to improve the welfare of dairy goats and sheep. However, studies in this field remain scattered, and its effects on productivity are unclear. Objectives: To evaluate the effects of environmental enrichment on behavioral, physiological, and productive parameters in dairy goats and sheep. Data sources: Scopus and Web of Science were searched for studies published from 2010 to 2025. Study eligibility criteria: Experimental or observational peer-reviewed studies comparing enriched vs. non-enriched housing in dairy goats or sheep, reporting on welfare or productivity outcomes. Methods: This review followed PRISMA 2020 guidelines and the PICO framework. Two independent reviewers screened and extracted data. Risk of bias was assessed with the SYRCLE tool. Results: Thirteen studies were included, mostly with goats. Physical, sensory, and social enrichments showed benefits for behavior (e.g., activity, fewer stereotypies) and stress physiology. However, results varied by social rank, enrichment type, and physiological stage. Only three studies assessed productive parameters (weight gain in kids/lambs); none evaluated milk yield or quality. Limitations: Most studies had small samples and short durations. No meta-analysis was conducted due to heterogeneity. Conclusions: Environmental enrichment can benefit the welfare of dairy goats and sheep. However, evidence on productivity is scarce. Long-term studies are needed to evaluate its cost-effectiveness and potential impacts on milk yield and reproductive performance. Full article

Early childhood forest and nature education plays a vital role in shaping values and promoting sustainability throughout life. Conceptualized in Denmark, forest and nature childcare groups have been established in Austria for over 20 years, contributing to mental well-being and supporting both Education for Sustainable Development (ESD) and Early Childhood Education and Care (ECEC). With increasing demand for childcare and a growing disconnect from nature—factors linked to physical and mental health challenges—there is a pressing need to expand these groups and integrate them into formal legal frameworks. This study examines the organization, staffing, infrastructure, risk prevention, and hygiene of 79 Austrian forest and nature kindergarten groups, identifying key areas of improvement to ensure safe access for all children, including those in public childcare. A semi-standardized online survey of 72 groups was analyzed using descriptive and statistical methods, including a Spearman correlation, Kruskal–Wallis test, Chi-square test, and ANOVA. Results revealed three main infrastructure types—house, container/trailer, and tipi—with houses offering the most comprehensive facilities. The ANOVA indicated significant effects of sponsorship type (p < 0.01), caregiver numbers (p < 0.001), and their interaction (p < 0.05) on half-day care costs. Currently, legal frameworks exist only in Tyrol and Salzburg. Broader access requires standardized infrastructure and risk assessment guidelines, collaboratively developed with stakeholders, to ensure safety and inclusivity in Austrian forest and nature childcare groups. Full article

The emission and presence of volatile organic compounds (VOCs) in the indoor air of houses and factories has been a growing topic of debate in the industry and related research fields. Given the extended times people in modern society spend indoors, monitoring VOCs is crucial due to the associated potential health hazards, with formaldehyde being particularly noteworthy. Wood and wood-based panels (WBPs) (the latter constituting a significant segment of the wood-transforming industry, being widely used in furniture, construction, and other applications) are known sources for the emission of VOCs to indoor air. In the case of the WBPs, the emission of VOCs depends on the type and species of wood, together with industrial processing and addition of additives. This review integrates perspectives on the production processes associated with WBPs, together with the evolving global regulations, and thoroughly examines VOC sources associated with WBPs, health risks from exposure, and current analytical methods utilized for VOC detection. It comprises an overview of the WBP industry, providing relevant definitions, descriptions of manufacturing processes and adhesive use, analysis of legal constraints, and explanations of VOC source identification and describing analysis techniques utilized for VOCs in WBPs. Full article