Search Results (762)

Background: Symbiotic fungi play essential roles throughout the entire cycle of orchid plants, including seed germination, seedling development, and maturation. Dendrobium officinale Kimura & Migo (Orchidaceae) (D. officinale) is a rare and highly valued traditional Chinese medicinal herb. Currently, artificial breeding using tissue culture technology is widely adopted and essential in the Dendrobium industry; however, this approach may impair or disrupt the plant’s ability to establish and maintain symbiotic relationships with mycorrhizal fungi. Methods: In this study, the fungal endophyte community (FEC) in the roots of D. officinale cultivated under four different modes was analyzed using high-throughput sequencing. Correlation analyses were also carried out to examine the relationships between bioactive compounds and the FEC. Results: (1) The FEC in D. officinale roots was dominated by Ascomycota and Basidiomycota, with significant differences in abundance, diversity, and community structure among cultivation modes; (2) the FEC under greenhouse cultivation differed significantly from those under tree epiphytic cultivation in terms of fungal nutritional types and dominant taxa; (3) six major mycorrhizal fungal taxa were identified in Dendrobium roots, although non-mycorrhizal fungi accounted for approximately 97% of the community; and (4) polysaccharide content in Dendrobium stems was positively correlated with certain root fugal endophytes (Exophiala, alaromyces, Pseudodactylaria, and Fellomyces). Conclusions: This study provides a foundation for understating the growth of D. officinale under different cultivation modes and highlights the relationship between bioactive compound accumulation and fungal endophyte communities. Full article

The adaptive and coordinated development of sustainable tourism and land-use efficiency is of great significance to regional sustainable development. Taking China as the study area, this research employs methods such as the CRITIC weighting method, the super-efficiency SBM model, the adaptive model and the PVAR model to examine the Spatio-temporal evolution, adaptive relationship and interactive responses between the levels of sustainable tourism and land-use efficiency across China’s 31 provinces from 2013 to 2023. The study found that: (1) The level of sustainable tourism exhibits a fluctuating upward trend. The development level in the eastern and central regions is higher than in the western and northeastern regions. Land use efficiency, with 2015 as a turning point, first declined and then fluctuated upwards. Regions with high efficiency are concentrated around Beijing and Shanghai. (2) The adaptive development between sustainable tourism and land-use efficiency is positive, with the eastern and central regions exhibiting a higher degree of adaptation than the western and northeastern regions. (3) Sustainable tourism and land-use efficiency exhibit a bidirectional interactive relationship, with each serving as a Granger cause for the other. The impact of sustainable tourism on land-use efficiency is greater than that of land-use efficiency on sustainable tourism. Full article

The convergence of Software-Defined Networking (SDN) and Blockchain (BC) creates a symbiotic relationship in which SDN’s programmable global visibility complements BC’s decentralized, immutable trust model to address critical cybersecurity vulnerabilities and cyber attacks. Addressing the fragmentation in the current literature, this study rigorously investigates BC and SDN (B-SDN) integration with the primary objectives of: (1) differentiating impacts across varied sectors, including the Internet of Things (IoT), Smart Grids, and Vehicular Ad Hoc Networks (VANETs) and more; (2) analyzing critical performance metrics such as energy efficiency and scalability; (3) classifying mitigation, detection, and prevention schemes for specific threats; (4) examining novel Artificial Intelligence (AI) methods; and (5) identifying open challenges and future research directions. Methodologically, this study conducts a survey of state-of-the-art B-SDN studies to investigate six key areas: Industry-specific applications, security mechanisms, defense strategies, defenses against specific attacks, AI integration, and implementation performance. The findings demonstrate that B-SDN integration shows strong potential in simulated and prototype environments to mitigate specific high-impact threats, such as Distributed Denial of Service (DDoS), Man-in-the-Middle (MiTM), and spoofing, across various domains, including IoT, 5G/6G, VANETS, and Smart Grid. Despite the benefits and advantages promised by B-SDN, several limitations continue to exist, including the latency–security trade-off inherent to consensus protocols and scalability constraints in large-scale deployments. Finally, open research challenges persist in AI-driven automation, particularly in Federated Learning (FL) and in the development of standardized interoperability protocols required to enable the transition from conceptual models to operational systems. Full article

At the present stage, driven by China’s “Three Types of Land” reform policies, the inter-action between urban and rural construction land (URCL) has evolved from an administratively dominated allocation structure toward a market-oriented one. Under the impetus of urban–rural transition, China’s urban and rural construction land has developed a symbiotic relationship characterized by internal factor mobility and external environmental constraints. This study develops a symbiosis indicator system and applies a logistic model to quantitatively evaluate the symbiotic relationship between urban construction land and rural construction land from 2009 to 2024 and to trace its evolutionary patterns. The results reveal the following: The symbiotic relationship gradually transitions from competitive symbiosis to mutualistic symbiosis, yet it exhibits frequent fluctuations and occasional transient reversions to competition, indicating that mutualism remains unstable. Urban construction land dominates during competitive symbiosis, whereas rural construction land dominates during mutualistic symbiosis; urban dominance within mutualistic phases occurs only sporadically. The marketization of rural construction land (measured by the leased and sold area and associated income) accelerates efficient urban—rural cooperation; land expropriation and the urbanization rate are key drivers shifting the relationship from competition to mutualism. However, excessive reliance on urbanization may impede the market structure of rural construction land and disrupt the established mutualistic pattern. Full article

As urban–rural integration deepens, the growth of suburban integrated villages, as the key link between urban–rural factor flow and coordinated development, is affected by the asymmetric reciprocal symbiosis between urban and rural areas. Utilizing urban–rural integration and sustainable development theories, this study selected twenty-four suburban integrated villages in the Daoli District of Harbin as its research object. It concentrates on the township–village micro-symbiotic unit, formulates a two-dimensional evaluation framework of “village endogenous + township linkage,” and utilizes quantitative methodologies, including the coupling coordination degree model, the obstacle degree model, and sensitivity analysis, to identify the factors hindering the expansion of these villages and propose targeted strategies according to villages’ coordination levels. The results show that 79% of samples exhibit imbalanced coordination; although spatial constraints are the primary hindrances to village systemic coordination, balanced development of spatial, economic, social and ecological subsystems remains essential; the developmental constraints of the samples stem from weak village developmental foundations and insufficient township–village radiation linkages. This study enhances the township–village symbiosis micro-research framework and functions as a reference for the classification planning of suburban integrated villages and the sustainable amalgamation of urban and rural development. Full article

Enterprise green transformation is a strategic response to emerging development concepts and high-quality growth, as well as a key approach to achieving symbiotic integration between firms and their social environment. Using a sample of Chinese A-share listed companies from 2008 to 2023, this paper examines the relationship between corporate social responsibility (CSR) disclosure and green transformation. It further explores the underlying mechanisms, focusing on the role of patient capital as an external governance mechanism within the green governance environment. Empirical results show that CSR disclosure significantly promotes corporate green transformation. Mechanism tests reveal that this effect operates through two channels: alleviating agency costs and easing financing constraints, with patient capital playing a positive moderating role. Additional analyses indicate that the promoting effect of CSR disclosure on green transformation is particularly pronounced in competitive and polluting industries. Full article

The fragmentation of resources, ecological imbalance, and the disintegration of essential factors in modern urban–rural development have become core bottlenecks constraining the achievement of global sustainable development goals. Existing research often focuses on static analysis or single-dimensional exploration, making it difficult to fully reveal the evolutionary patterns of urban–rural sustainable development systems and the core mechanisms of digital empowerment. This study adopts ecological wisdom as a theoretical perspective, introduces system dynamics methods, and constructs a three-dimensional linkage system simulation model of digital technology, factor circulation, and ecological wisdom capital. Based on model simulation data, a 70-year long-cycle simulation is conducted to explore the leverage effect of digital technology and the self-organizing evolutionary mechanisms of the system. The findings are as follows: First, the transformation of urban–rural sustainable development from fragmentation to synergy is essentially a self-organizing phase transition process. Within the system, digital technology, factor circulation, and ecological wisdom capital form a mutually beneficial symbiotic relationship through positive feedback mechanisms, driving the system toward higher-order evolution. Second, key variables such as community participation, urban–rural ecological resilience, and development pressure exhibit significant threshold effects. Only when the investment scale and institutional guarantees for these variables reach critical values can the system’s path dependence be broken, significantly driving synergistic transformation. Third, as an exogenous lever, digital technology can break down barriers to factor segmentation, reduce energy loss during system transformation, and achieve systematic integration of urban–rural resources, public services, and ecological capital with lightweight investment, serving as the core breakthrough for promoting synergistic transformation. This study integrates ecological wisdom and digital technology into the system dynamics analysis framework of urban–rural synergistic transformation, clarifies the dynamic transmission pathways of digital technology empowering urban–rural synergy, overcomes the limitations of traditional static research, enriches the cross-disciplinary application of ecological wisdom and system dynamics in the field of urban–rural development, and provides a scientific policy basis for the mutual construction of the digital economy, ecological protection, and urban–rural integration. Full article

The symbiotic relationship between soybean and rhizobia facilitates nodulation and nitrogen fixation, providing a sustainable nutrient supply for increasing crop yields and reducing chemical fertilizer use. However, comparative studies on the conservation and strain-specificity of host gene expression regulated by different rhizobial strains remain limited. Here, we performed a comparative analysis between the previously isolated strain, Bradyrhizobium ottawaense Bott 59, and the model strain, Bradyrhizobium diazoefficiens USDA 110. Symbiotic phenotypes were evaluated after inoculation, and a root transcriptomic analysis was conducted at 3 dpi to assess early molecular responses. At 21 dpi, Bott 59-inoculated plants outperformed plants inoculated with USDA 110 in nodule number, nitrogenase activity, and biomass. Transcriptomic analysis revealed conserved host responses to both rhizobial strains, including NIN-mediated signaling, AON signaling, and the biosynthesis of phenylpropanoids and brassinosteroids. Further analysis revealed that Bott 59 specifically induced the expression of genes involved in isoflavonoid and flavonoid biosynthesis, including those encoding I2H, and HI4OMT. Moreover, Bott 59 triggered more pronounced transcriptional reprogramming in auxin, cytokinin, and jasmonic acid signaling pathways, along with differential expression of a broader set of transcription factor genes. Collectively, this study systematically unravels the conserved and strain-specific transcriptional regulatory events underlying host–rhizobium interactions. Our findings provide valuable theoretical insights and transcriptomic resources for further dissecting the molecular mechanisms of symbiotic nitrogen fixation (SNF), as well as for the targeted genetic improvement of crop nodulation and nitrogen fixation efficiency. Full article

Wolbachia is an obligate endosymbiotic alphaproteobacterium that is widely distributed among insects. It also infects the European orthopteran Chorthippus parallelus parallelus (Cpp). In this subspecies, Wolbachia induces a reproductive barrier through uni- and bidirectional cytoplasmic incompatibilities. Recently, we found that it also modifies the expression of genes related to essential physiological pathways in Cpp. Here, we have analysed the influence of Wolbachia infection on the epigenetic profiles in Cpp gonads of infected and uninfected males and females, since they constitute Wolbachia’s main target. We characterised de novo nine genes related to epigenetic mechanisms and their transcriptional activity, together with global DNA methylation levels. The results indicate that Wolbachia influences the epigenetic mechanisms in Cpp mainly in females, inducing the expression of genes related to histone deacetylation and reducing the global DNA methylation percentage. This study provides the first evidence of Wolbachia’s ability to alter epigenetic processes in Cpp, increasing our understanding of this symbiotic relationship, with potential implications for the induced reproductive isolation within and between subspecies of C. parallelus. It also offers new insights into the molecular basis of host–symbiont biology in a group for which this information is rather scarce. Full article

Fungal endophytes are symbiotic microorganisms that establish strong relationships inside plant tissues, providing potential advantages, especially in grasses, by enhancing tolerance to both abiotic and biotic stresses. This review investigates the molecular mechanisms through which fungal endophytes mediate stress tolerance, targeting host–pathogen interactions. By modulating pathogen-associated molecular patterns (PAMPs), damage-associated molecular patterns (DAMPs), and effector proteins, fungal endophytes may contribute to priming the plant’s immune system, enhancing its resistance to pathogen invasion. Moreover, endophyte colonization regulates core processes such as osmotic regulation, reactive oxygen species (ROS) detoxification, and secondary metabolite biosynthesis that enable plants to tolerate environmental stresses like drought, heat, and salinity. The review highlights the impact of endophytes on immune priming, systemic acquired resistance (SAR), and the regulation of non-coding RNAs that regulate host gene networks associated with stress tolerance. Furthermore, the integration of advanced multi-omics techniques genomics, transcriptomics, proteomics, metabolomics, and fluxomics has revealed emerging insights into the genetic and metabolic pathways driving these symbiotic associations. However, grass-specific molecular datasets remain limited, and the consistency of endophyte-mediated tolerance across host species and environmental conditions is not yet fully resolved. Fungal endophytes increase grass stress resilience through coordinated pathogen recognition, RNA regulation, and metabolic reprogramming while AI-assisted multi-omics approaches are emerging as tools for identifying candidate regulatory networks, although empirical validation in grass–endophyte systems remains limited. Together, these advances highlight the potential for climate-smart and sustainable crop improvement. Future research integrating functional genomics, field validation, and biosafety assessment will be essential for translating endophyte-based strategies into reliable agricultural applications. Full article

The rapid emergence of multi-drug resistant (MDR) bacteria has become a major health concern, driving the need to identify new antimicrobial resources. Recently, endophytes, inhabiting in internal tissues of medicinal plants, have drew important interest from the scientific community, as reservoirs of bioactive metabolites. Numerous studies highlight the symbiotic relationship between plants and their endophytes, in which these microorganisms produce antimicrobial compounds, helping the host plant’s defense against pathogens. Plantago major (commonly known as plantain) is widely recognized for its therapeutic properties, especially for its antimicrobial properties. In this study, endophytic fungi were isolated from Plantago major, morphologically characterized and identified using ITS sequencing. Their antibacterial activity was assessed using the agar diffusion assay. In total, 21 endophytic fungal isolates were obtained from different plant tissues, including leaves, stems, roots, and flowers. Antibacterial assays against methicillin-resistant Staphylococcus aureus (MRSA) were investigated on PDA, SDA, and CDA media. Amongst the isolates, nine strains (MD-H1, MD-L1, MD-L2, MD-L3, MD-L4, MD-L5, MD-R1, MD-T1, MD-T2, and MD-T10) showed medium to strong antibacterial effects, with inhibition zones exceeding 15 mm. The result suggests that endophytic fungi associated with Plantago is a valuable source of anti-MRSA compounds. Further work will focus on identifying the secondary metabolites responsible for this activity and elucidating their chemical structures, providing a basis for the development of new potent antibiotic agents. Full article

Endophytic fungi (EF) inhabit internal plant tissue in a mutually beneficial symbiotic relationship with their host plant. EF synthesizes metabolites that are structurally similar or identical to those found in their host plants, which include alkaloids, flavonoids, terpenoids, phenolic compounds, polysaccharides, proteins, lipids, and organic acids. These molecules have promising therapeutic effects, such as antimicrobial, antioxidant, anti-inflammatory, and antitumor activities. Wound healing has earned attention in recent years because of its relation to chronic pathological diseases. This systematic review scanned the available scientific literature database about the wound-healing properties of EF biomolecules. Amongst 994 works, 24 were screened after abstract and full-text reading. The studies were published between 2014 and 2026, in twelve countries. In total, 16 studies presented in vivo assays, 11 studies presented in vitro assays, and 3 studies presented both assays. Most studies identified molecules, which include melanin, benzoic acid, terpenes, sesquiterpenes (purpurolide), extracellular polysaccharides, exopolysaccharides, carotenoids, fatty acids, proteins, pyrones, quinones, and hydrocarbon acids, among others. A meta-analysis was not conducted due to high heterogeneity across extracts, methodologies, and outcomes. All studies showed wound-healing properties from EF extracts. The findings suggest a positive effect of EF extracts on wound-healing properties and the need for standardized in vitro and in vivo protocols. Full article

Armillaria species hold significant ecological and economic importance and they play a vital role in the growth of traditional Chinese medicine Gastrodia elata (G. elata). In this study, we assembled and compared the mitochondrial genomes (mitogenomes) of six Armillaria mellea (Vahl) P. Kumm. (A. mellea) strains isolated from the main G. elata-producing region of Hanzhong, China. The internal transcribed spacer (ITS) sequencing confirmed that all six strains form a monophyletic clade. Their mitogenomes (120,775 to 120,839 bp) exhibit a highly conserved architecture, each containing 16 protein-coding genes (PCGs), 23 open reading frames (ORFs), 27 tRNAs, and two rRNAs. Codon usage and amino acid frequency were strikingly similar among the six strains, with a strong AT bias. In contrast, comparisons with other Armillaria species revealed marked differences in gene order, repeat structures, and selection pressures. Phylogenetic analyses based on PCGs further resolved the close relationship among the six strains while highlighting distinct molecular variation across species. On the whole, these findings demonstrate that A. mellea strains co-evolving with G. elata maintain a highly uniform mitochondrial genome architecture, suggesting strong purifying selection or recent divergence within this symbiotic population. The pronounced differences from other Armillaria species at the levels of gene arrangement and selection pressure imply that mitochondrial gene rearrangement may have accompanied species diversification in the genus. By providing the first complete mitogenomes of A. mellea from a major G. elata cultivation area, this study not only expands the genomic resources for Armillaria but also establishes a foundation for understanding how mitochondrial variation might influence fungal growth, adaptation, and symbiotic efficiency with G. elata. Full article

Symbiotic Intelligence (SI) rethinks dominant evolutionary narratives within Western artificial intelligence (AI) development through a practice-led research methodology centred on co-creating with mycelium. This research investigates how living mycelium can inform and reframe prevailing AI narratives, particularly those shaped by evolutionary logics. These narratives, found in developer manifestoes and futurological discourse, often frame intelligence within competitive, deterministic paradigms rooted in social Darwinism and invoke eugenicist ideas such as the g-factor in intelligence. Through the creation of responsive art installations, the project positions mycelium as a material and conceptual collaborator, opening new spaces for dialogue. This article inverts the curatorial strategy of incorporating AI technology into artistic practices. Instead, we show how arts-led ‘making’ practices can generate new narratives that propose alternative ethical frameworks and sustainable directions for technological development. We argue that a direct, generative but non-deterministic relationship exists between AI narratives and the technical actualisation of AI. Specifically, SI contends that: (i) evolutionary narratives underpin Western AI imaginaries; (ii) these often reflect reductive social Darwinist models; (iii) counter-narratives grounded in collective assemblage and symbiotic intelligence are essential for shaping more complex and sustainable AI futures. Full article

The gastrointestinal mucus barrier (GIMB) is a gelatinous structure consisting primarily of mucins, water, and cathelicidin. Such a structure is the first line of defense against pathogens in the intestinal cavity and acts an important environment for the survival and reproduction of symbiotic flora. Mucin is mainly synthesized and secreted by intestinal goblet cells, forming a slime layer with different structures throughout the intestinal tract. The process of mucin synthesis and secretion is regulated by many factors, and there are some differences in the physical and chemical properties of the GIMB among animal species. Furthermore, recent studies have shown a close relationship among the mucus barrier, gastrointestinal diseases, and tumors. Soybean agglutinin (SBA) is a major glycoprotein in soybean that is closely related with the detection, prevention, and treatment of disease and cancer. Current studies indicate a close relationship between SBA and the GIMB, particularly at the molecular level and through species-specific differences in mucin glycan structures. Existing evidence shows that these differences affect the binding affinity and antinutritional effects of SBA. The novel relations between SBA and GIMB may become new targets for disease treatment. Full article