Search Results (61)

Odors from pet cats can negatively affect the quality of life of cat owners. The diverse bioactive compounds in plant extracts make them a promising candidate for effective odor reduction. This study evaluated twelve plant extracts for deodorizing efficacy via in vitro fermentation tests. Rosemary extract and licorice extract exhibited better deodorizing effects, with fractions of rosemary extract below 100 Da demonstrating the most effective deodorizing performance. Based on these findings, subsequent feeding trials were conducted using rosemary extract and its fractions below 100 Da. In the feeding trial, adult British Shorthair cats were divided into three groups (Control Check, RE, and RE100) and housed in a controlled-environment respiration chamber for 30 days. Measurements included odor emissions, fecal and blood physicochemical parameters, immune parameters, microbiota composition based on 16S rRNA sequencing, and metabolome analysis. The results of the feeding trial indicated that rosemary extract significantly reduced ammonia and hydrogen sulfide emissions (46.84%, 41.64%), while fractions below 100 Da of rosemary extract achieved even greater reductions (55.62%, 53.87%). Rosemary extract regulated the intestinal microbial community, significantly increasing the relative abundance of the intestinal probiotic Bifidobacterium (p < 0.05) and reducing the population of sulfate-reducing bacteria (p < 0.05). It also significantly reduced urease and uricase activities (p < 0.05) to reduce ammonia production and inhibited the degradation of sulfur-containing proteins and sulfate reduction to reduce hydrogen sulfide emissions. Furthermore, rosemary extract significantly enhanced the immune function of British Shorthair cats (p < 0.05). This study suggests that rosemary extract, particularly its fractions below 100 Da, is a highly promising pet deodorizer. Full article

While rhizosphere microbiome functions in saline soils are well documented, complementary microbial strategies between pioneer and late-successional halophytes remain unexplored. Here, we used 16S rRNA sequencing and FAPROTAX functional prediction to compare the rhizosphere bacterial communities of two key halophytes—Suaeda glauca and Phragmites communis—in a reclaimed coastal wetland. The results demonstrate that both plants significantly restructured microbial communities through convergent enrichment of stress-tolerant taxa (Firmicutes, Pseudomonas, Bacillus, and Planococcus) while suppressing sulfur-oxidizing bacteria (Sulfurovum and Thiobacillus). However, they exhibited distinct microbial specialization: S. glauca uniquely enriched organic-matter-degrading taxa (Promicromonospora and Zhihengliuella) and upregulated aromatic compound degradation (2.29%) and ureolysis (0.86%) according to FAPROTAX analysis, facilitating carbon mobilization in early successional stages. Notably, P. communis selectively recruited nitrogen-cycling Serratia, with increased nitrate respiration (3.51% in P. communis vs. 0.91% in S. glauca) function, reflecting its higher nitrogen demand. Environmental factors also diverged: S. glauca’s microbiome correlated with potassium and sodium, whereas P. communis responded to phosphorus and chloride. These findings uncover distinct microbial recruitment strategies by halophytes to combat saline stress—S. glauca–P. communis synergy through microbial carbon-nitrogen coupling—offering a template for consortia design in saline soil restoration. Full article

To better inform the public about ambient air quality and associated health risks and prevent cardiovascular and chronic respiratory diseases in Macau, the local government authorities apply the Air Quality Index (AQI) for air quality management within its jurisdiction. The application of AQI requires first determining the sub-indices for several pollutants, including respirable suspended particulates (PM₁₀), fine suspended particulates (PM_2.5), nitrogen dioxide (NO₂), ozone (O₃), sulfur dioxide (SO₂), and carbon monoxide (CO). Accurate prediction of AQI is crucial in providing early warnings to the public before pollution episodes occur. To improve AQI prediction accuracy, deep learning methods such as artificial neural networks (ANNs) and long short-term memory (LSTM) models were applied to forecast the six pollutants commonly found in the AQI. The data for this study was accessed from the Macau High-Density Residential Air Quality Monitoring Station (AQMS), which is located in an area with high traffic and high population density near a 24 h land border-crossing facility connecting Zhuhai and Macau. The novelty of this work lies in its potential to enhance operational AQI forecasting for Macau. The ANN and LSTM models were run five times, with average pollutant forecasts obtained for each model. Results demonstrated that both models accurately predicted pollutant concentrations of the upcoming 24 h, with PM₁₀ and CO showing the highest predictive accuracy, reflected in high Pearson Correlation Coefficient (PCC) between 0.84 and 0.87 and Kendall’s Tau Coefficient (KTC) between 0.66 and 0.70 values and low Mean Bias (MB) between 0.06 and 0.10, Mean Fractional Bias (MFB) between 0.09 and 0.11, Root Mean Square Error (RMSE) between 0.14 and 0.21, and Mean Absolute Error (MAE) between 0.11 and 0.17. Overall, the LSTM model consistently delivered the highest PCC (0.87) and KTC (0.70) values and the lowest MB (0.06), MFB (0.09), RMSE (0.14), and MAE (0.11) across all six pollutants, with the lowest SD (0.01), indicating greater precision and reliability. As a result, the study concludes that the LSTM model outperforms the ANN model in forecasting air pollutants in Macau, offering a more accurate and consistent prediction tool for local air quality management. Full article

The BCS1L gene encodes a mitochondrial chaperone which inserts the Fe₂S₂ iron–sulfur Rieske protein into the nascent electron transfer complex III. Variants in the BCS1L gene are associated with a spectrum of mitochondrial disorders, ranging from mild to severe phenotypes. Björnstad syndrome, a milder condition, is characterized by sensorineural hearing loss (SNHL) and pili torti. More severe disorders include Complex III Deficiency, which leads to neuromuscular and metabolic dysfunctions with multi-systemic issues and Growth Retardation, Aminoaciduria, Cholestasis, Iron Overload, and Lactic Acidosis syndrome (GRACILE). The severity of these conditions varies depending on the specific BCS1L mutation and its impact on mitochondrial function. This study describes a 27-month-old child with SNHL, proximal renal tubular acidosis, woolly hypopigmented hair, developmental delay, and metabolic alterations. Genetic analysis revealed a homozygous BCS1L variant (c.38A>G, p.Asn13Ser), previously reported in a patient with a more severe phenotype that, however, was not functionally characterized. In this work, functional studies in a yeast model and patient-derived fibroblasts demonstrated that the variant impairs mitochondrial respiration, complex III activity (CIII), and also alters mitochondrial morphology in affected fibroblasts. Interestingly, we unveil a new possible mechanism of pathogenicity for BCS1L mutant protein. Since the interaction between BCS1L and CIII is increased, this suggests the formation of a BCS1L-containing nonfunctional preCIII unable to load RISP protein and complete CIII assembly. These findings support the pathogenicity of the BCS1L c.38A>G variant, suggesting altered interaction between the mutant BCS1L and CIII. Full article

The Okinawa Trough (OT) has been a focus of scientific research for many years due to the presence of vibrant hydrothermal and cold seep activity within its narrow basin. However, the spatial distribution and environmental drivers of microbial communities in OT sediments remain poorly understood. The present study aims to address this knowledge gap by investigating microbial diversity and abundance at ten different sampling sites in a transitional zone between hydrothermal vents and cold seeps in the OT. The microbial community at two sampling sites (G08 and G09) in close proximity to hydrothermal vents showed a high degree of similarity. However, lower bacterial and archaeal abundances were found in these sites. The archaeal groups, classified as Hydrothermarchaeota and Thermoplasmata, showed a comparatively higher relative abundance at these sites. In addition, ammonia-oxidizing archaea (AOA), from the family Nitrosopumilaceae, were found to have a higher relative abundance in the OT surface sediments at sampling sites G03, G04, G05, G06, and G07. This result suggests that ammonia oxidation may be actively occurring in these areas. Furthermore, Methylomirabilaceae, which are responsible for methane oxidation coupled with nitrite reduction, dominated three sampling sites (G07, G08, and G09), implying that N-DAMO may play an important role in mitigating methane emissions. Using the FAPROTAX database, we found that predicted prokaryotic microbial functional groups involved in methyl-reducing methanogenesis and hydrogenotrophic methanogenesis were most abundant at sites G08 and G09. At sampling sites G01 and G02, functional groups such as hydrocarbon degradation, methanotrophy, methanol oxidation, denitrification, sulfate respiration, and sulfur oxidation were more abundant. Nitrogen content is the most important environmental factor determining the bacterial and archaeal communities in the OT surface sediments. These results expand our knowledge of the spatial distribution of microbial communities in the transitional zone between hydrothermal vents and cold seeps in the OT. Full article

Glucosinolates (GSLs) are nitrogen/sulfur-containing glycosides widely present in the order of Brassicales, particularly in the Brassicaceae family. Camelina (Camelina sativa (L.) Crantz) is an oilseed plant belonging to this family. Its seeds, in addition to a distinctive fatty acid composition, contain three aliphatic GSLs: glucoarabin, glucocamelinin, and homoglucocamelinin. Our study explored the impact of these GSLs purified from Camelina press cake, a by-product of Camelina oil production, on yeast chronological aging, which is the established model for simulating the aging of post-mitotic quiescent mammalian cells. Supplementing yeast cells with GSLs extends the chronological lifespan (CLS) in a dose-dependent manner. This enhancement relies on an improved mitochondrial respiration efficiency, resulting in a drastic decrease of superoxide anion levels and an increase in ATP production. Furthermore, GSL supplementation affects carbon metabolism. In particular, GSLs support the pro-longevity preservation of TCA cycle enzymatic activities and enhanced glycerol catabolism. These changes contribute positively to the phosphorylating respiration and to an increase in trehalose storage: both of which are longevity-promoting prerequisites. Full article

An aerobic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium, strain AK1^T, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka, Russia. The cells of the new isolate were Gram-negative motile rods with a single polar flagellum. Strain AK1^T grew at 37–55 °C (optimum 50 °C) with 0–1.0% NaCl (optimum 0%) and within the pH range 4.8–7.0 (optimum pH 5.2–5.5). The new isolate was able to grow by aerobic respiration with sulfide, sulfur, or thiosulfate as the electron donor and HCO₃⁻/CO₂ as the carbon source. The major fatty acids were C16:0, C17:1 Δ, and C16:1 ω7c. The respiratory lipoquinone was ubiquinone UQ-8. The size of the genome and genomic DNA G+C content of the strain AK1^T were 2.55 Mb and 64.0%, respectively. The closest 16S rRNA gene sequence of a validly published species belonged to Thiobacter subterraneus C55^T (97.94% identity). According to the 16S rRNA gene sequence-based and conserved protein sequences-based phylogenetic analyses, strain AK1^T represented a distinct lineage of the genus Thiobacter within a new family, Thiobacteraceae of the order Burkholderiales. As inferred from the morphology, physiology, chemotaxonomy, and phylogeny, strain AK1^T ought to be recognized as a novel species for which we propose the name Thiobacter aerophilum sp. nov. The type strain is AK1^T (=CGMCC 1.18099^T = UQM 41819^T). Full article

Organic farming encourages soil management practices that can improve soil health and fertility by increasing soil organic matter inputs and system sustainability. This study evaluated the effect of three years of continuous organic farming and intercropping orchard treatments on soil microbial diversity, microbial enumeration, respiration, soil fertility and fruit yields. Organic management resulted in higher soil organic matter content, Olsen P, and water holding capacity, but did not affect soil pH, electrical conductivity (EC), K, or Na levels. Growth parameters measured on all fruit trees were not significantly different among treatments. The enumeration of bacteria was significantly higher in organic plots when compared to conventionally managed plots. Soil respiration and substrate-induced respiration were significantly higher in the organic diverse plots in comparison to both conventional systems. The genomic analysis of prokaryotes (16S rRNA) and eukaryotes/fungi (ITS) revealed a significantly higher number of taxa, Shannon H index, and Equitability index in the organic systems for both prokaryotes and eukaryotes, in comparison to conventional farming, all of which are indicators of system sustainability. The relative abundance of Operational Taxonomic Units (OTUs) previously reported as diazotrophs, denitrifiers, or involved in the sulfur cycle, as well as Arbuscular Mychorrizae Fungi (AMF)/glomeromycotan, were highest in the organically managed soils than in the conventional plots. A multivariate correlation network clustering revealed that the microbial communities within the organic and conventional soils had strong dissimilarities regarding soil microbial niches. Our work provides evidence that organic management can be used for increasing soil microbial diversity and soil health, leading to higher levels of sustainability in fruit orchard systems. Full article

Overdose of acetaminophen (APAP) produces fulminant hepatic necrosis. The underlying mechanism of APAP hepatotoxicity involves mitochondrial dysfunction, including mitochondrial oxidant stress and the onset of mitochondrial permeability transition (MPT). Reactive oxygen species (ROS) play an important role in APAP-induced hepatotoxicity, and iron is a critical catalyst for ROS formation. This review summarizes the role of mitochondrial ROS formation in APAP hepatotoxicity and further focuses on the role of iron. Normally, hepatocytes take up Fe³⁺-transferrin bound to transferrin receptors via endocytosis. Concentrated into lysosomes, the controlled release of iron is required for the mitochondrial biosynthesis of heme and non-heme iron-sulfur clusters. After APAP overdose, the toxic metabolite, NAPQI, damages lysosomes, causing excess iron release and the mitochondrial uptake of Fe²⁺ by the mitochondrial calcium uniporter (MCU). NAPQI also inhibits mitochondrial respiration to promote ROS formation, including H₂O₂, with which Fe²⁺ reacts to form highly reactive •OH through the Fenton reaction. •OH, in turn, causes lipid peroxidation, the formation of toxic aldehydes, induction of the MPT, and ultimately, cell death. Fe²⁺ also facilitates protein nitration. Targeting pathways of mitochondrial iron movement and consequent iron-dependent mitochondrial ROS formation is a promising strategy to intervene against APAP hepatotoxicity in a clinical setting. Full article

The extensive metabolic diversity of microalgae, coupled with their rapid growth rates and cost-effective production, position these organisms as highly promising resources for a wide range of biotechnological applications. These characteristics allow microalgae to address crucial needs in the agricultural, medical, and industrial sectors. Microalgae are proving to be valuable in various fields, including the remediation of diverse wastewater types, the production of biofuels and biofertilizers, and the extraction of various products from their biomass. For decades, the microalga Chlamydomonas has been widely used as a fundamental research model organism in various areas such as photosynthesis, respiration, sulfur and phosphorus metabolism, nitrogen metabolism, and flagella synthesis, among others. However, in recent years, the potential of Chlamydomonas as a biotechnological tool for bioremediation, biofertilization, biomass, and bioproducts production has been increasingly recognized. Bioremediation of wastewater using Chlamydomonas presents significant potential for sustainable reduction in contaminants and facilitates resource recovery and valorization of microalgal biomass, offering important economic benefits. Chlamydomonas has also established itself as a platform for the production of a wide variety of biotechnologically interesting products, such as different types of biofuels, and high-value-added products. The aim of this review is to achieve a comprehensive understanding of the potential of Chlamydomonas in these aspects, and to explore their interrelationship, which would offer significant environmental and biotechnological advantages. Full article

Background: This study explores the role of leguminous crops like lima bean in enhancing soil quality and ecosystem stability. Despite existing studies on agronomic aspects, there is a significant research gap on its impact on soil organic matter level, microbial activity, soil health, and nutrient availability. Therefore, this study examines the capacity of lima bean to reactivate soil quality, focusing on its impact on soil organic matter level, microbial activity, soil health, and nutrient availability. Methods: The experimental area was set up in 2023 using three replicates and a randomized block design. Two treatments were used: lima bean-planted plots and control plots with various weeds and without lima bean. Post-harvest soil samples were collected from various agroecological zones and sterilely packed, and physical, chemical, and biological indices were examined. Results: lima bean significantly affected nutrients, enzymes, soil microbial respiration, and other markers. Amylase activity (0.41**) was positively correlated with urease activity (0.73**), while dehydrogenase activity positively correlated with both. Dehydrogenase activity was negatively correlated with total nitrogen (0.66**) and sulfur (0.60**). Lima bean significantly affected soil quality, with all locations showing higher ratings (55–77%) than wild land, except for location D (Ilora). A total of 70% of total nitrogen variation may be attributed to soil quality (r² = 0.696). Lima bean enhanced soil quality, potentially enhancing productivity and reducing dependence on inorganic nitrogen inputs. Conclusions: The symbiotic relationship between lima bean and nitrogen-fixing bacteria improves nutrient cycling, enhancing agricultural productivity and environmental conservation. Future research should explore the economic viability of integrating lima bean into crop rotations or agroforestry systems for sustainable agricultural practices, providing valuable information for farmers. Full article

Cysteine and its derivatives, including H₂S, can influence bacterial virulence and sensitivity to antibiotics. In minimal sulfate media, H₂S is generated under stress to prevent excess cysteine and, together with incorporation into glutathione and export into the medium, is a mechanism of cysteine homeostasis. Here, we studied the features of cysteine homeostasis in LB medium, where the main source of sulfur is cystine, whose import can create excess cysteine inside cells. We used mutants in the mechanisms of cysteine homeostasis and a set of microbiological and biochemical methods, including the real-time monitoring of sulfide and oxygen, the determination of cysteine and glutathione (GSH), and the expression of the Fur, OxyR, and SOS regulons genes. During normal growth, the parental strain generated H₂S when switching respiration to another substrate. The mutations affected the onset time, the intensity and duration of H₂S production, cysteine and glutathione levels, bacterial growth and respiration rates, and the induction of defense systems. Exposure to chloramphenicol and high doses of ciprofloxacin increased cysteine content and GSH synthesis. A high inverse relationship between log CFU/mL and bacterial growth rate before ciprofloxacin addition was revealed. The study points to the important role of maintaining cysteine homeostasis during normal growth and antibiotic exposure in LB medium. Full article

While crushed pinecone mulch holds promise as a beneficial material for blueberry cultivation, research on its effectiveness remains limited. Crop leaf characteristics can serve as parameters for assessing mulching effects, although there are several limitations, including the need to analyze various distinct characteristics separately. The combination of hyperspectral data and machine learning techniques is expected to enable the selection of only the most important features among these characteristics. In this study, we investigated the impact of various mulching treatments utilizing pine tree byproducts, including crushed pinecones. Mulching variations included non-mulching (NM), crushed pinecones (PCs), a mixture of crushed pinecones and sulfur (PCS), pine needles (PNs), and sulfur treatment (S). Conventional methods were employed to measure leaf growth (length and width) and physiological characteristics (chlorophyll content, chlorophyll fluorescence, and stomatal conductance). Hyperspectral reflectance was also measured, and classification models using Partial Least Squares Discriminant Analysis (PLS-DA) and eXtreme Gradient Boosting (XGBoost) were developed for crop characteristics, vegetation indices (VIs), visible and near-infrared (VNIR), and short-wave infrared (SWIR). The results showed that using crushed pinecones as the sole mulching material for blueberries, without sulfur treatment, had a positive impact on blueberry growth. The PC treatment exhibited a dual effect on plant growth by lowering the soil pH to 5.89 and maintaining soil moisture within the range of 26.33–35.20%. We observed distinct differences in soil inorganic nutrient content, with higher concentrations of organic matter, total nitrogen, and available P₂O₅ and K⁺, which positively influenced blueberry growth. Mulching treatments demonstrated superior physiological characteristics, with two classification models identifying stomatal conductance (gs) as a key parameter influencing treatment classification (VIP scores > 1 rank: 3, variable score rank: 1). The photochemical reflectance index (PRI) emerged as a major parameter among VIs, showing potential for measuring water stress (VIP scores > 1 rank: 2, variable score rank: 1). In the SWIR PLS-DA model, wavelength peaks were mainly observed in the O-H overtone (1410 nm, 1450 nm, 1930 nm, 1940 nm, and 2100 nm). Overall, crushed pinecones were found to positively impact the initial growth of blueberries by enhancing water status (plant respiration). Full article

Iron is an essential nutrient and necessary for biological functions from DNA replication and repair to transcriptional regulation, mitochondrial respiration, electron transfer, oxygen transport, photosynthesis, enzymatic catalysis, and nitrogen fixation. However, due to iron’s propensity to generate toxic radicals which can cause damage to DNA, proteins, and lipids, multiple processes regulate the uptake and distribution of iron in living systems. Understanding how intracellular iron metabolism is optimized and how iron is utilized to regulate other intracellular processes is important to our overall understanding of a multitude of biological processes. One of the tools that the cell utilizes to regulate a multitude of functions is the ligation of the iron–sulfur (Fe-S) cluster cofactor. Fe-S clusters comprised of iron and inorganic sulfur are ancient components of living matter on earth that are integral for physiological function in all domains of life. FeS clusters that function as biological sensors have been implicated in a diverse group of life from mammals to bacteria, fungi, plants, and archaea. Here, we will explore the ways in which cells and organisms utilize Fe-S clusters to sense changes in their intracellular environment and restore equilibrium. Full article