Search Results (36,227)

The escalating threat of antimicrobial resistance (AMR) and the environmental impact of industrial pollutants, particularly synthetic dyes, emphasize the pressing requirement for novel solutions. This study investigates the green synthesis of ZnO/Fe₂O₃ nanocomposites using Urtica dioica extract with the aim of achieving dual functionality as both antimicrobial agents and photocatalysts for pollutant degradation. The nanocomposites were synthesized with varying loads of Fe₂O₃ (5–50%) and characterized using X-ray diffraction (XRD) and diffuse reflectance spectroscopy (DRS). XRD analysis confirmed the presence of both the hexagonal wurtzite ZnO phase and the α-Fe₂O₃ hematite phase in all the composites, while DRS analysis revealed that the bandgap energy decreased progressively (from 1.89 to 1.72 eV) as the Fe₂O₃ content increased. The photocatalytic efficiency of the composites was evaluated by degrading methylene blue (MB), Congo Red (CR) and safranin O (SO) dyes under visible light. This demonstrated that the degradation performance depends on the composition, with the best activity being observed at 5% Fe₂O₃. Antioxidant activity was assessed using a DPPH• free radical scavenging assay. This showed that Urtica dioica extract exhibits superior radical scavenging capacity (maximum inhibition of 38%) compared to ZnO/Fe₂O₃ nanoparticles (maximum inhibition of 18%). The antibacterial efficacy against Pseudomonas aeruginosa was evaluated using direct confrontation and disk diffusion methods. This revealed that the activity was dose- and light-dependent, with enhanced performance under light exposure (10 mm inhibition zone) compared to dark conditions (1 mm). This study demonstrates the successful green synthesis of biphasic ZnO/Fe₂O₃ nanocomposites with promising photocatalytic and antimicrobial properties. While the results suggest possible synergistic interactions between the oxides, the underlying mechanisms, including potential charge transfer effects, require further investigation using advanced characterization techniques. Using Urtica dioica extract as a biogenic source provides a promising eco-friendly approach to synthesizing nanomaterials, with potential applications in wastewater treatment and the biomedical field. Full article

Canine mammary cancers (CMCs) are one of the most prevalent types of neoplasm in dogs, are frequently malignant, and display high tumour heterogeneity, making evaluating prognosis and predicting successful treatment outcomes difficult. In a previous pilot study, overexpression of the Wnt pathway-associated protein SFRP1 was found to correlate with negative metastasis status in CMCs at both mRNA and protein levels. To establish SFRP1 as a potential biomarker for CMC progression, additional verification of these results in an independent dataset is required, as well as an investigation as to whether SFRP1 expression in CMCs is associated with altered Wnt- or RANKL signalling pathways. In an independent verification cohort of 122 cases of archival CMC FFPE material, expression of SFRP1 was assessed by RT-qPCR and immunohistochemistry. The same tumours were further assessed for RANKL, phosphoROCK2, and NFkB-p65 protein expression. Our data verified that SFRP1 mRNA (p = 0.025) was negatively associated with metastasis status; however, differences in protein expression did not reach statistical significance (p = 0.139). Neither did SFRP1 significantly correlate with expression of any of the other proteins tested. Instead, a strong association was found for RANKL positivity with increased metastasis status (p < 0.001). Co-expression of SFRP1 significantly lowered the higher risk of metastatic spread when compared to RANKL_pos/SFRP1_neg CMCs (p = 0.033). Noticeably, all vascular-invasive cell clusters observed in tissue section vessels stained positive for RANKL. Our study identified RANKL expression as a strong marker for cancer progression with a strong link to vascular-invasive cells. However, SFRP1 expression may potentially suppress the pro-metastatic nature of RANKL_pos CMCs. Full article

Background: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 poses a significant zoonotic threat, largely due to its capacity to acquire and disseminate antimicrobial resistance through mobile genetic elements (MGEs). Ready-to-eat (RTE) foods may serve as critical interfaces for zoonotic spillover. However, genomic data on ST398-MRSA-Vc isolates from RTE foods remain scarce, leaving the characteristics of their MGEs largely unresolved. Methods: This study performed whole-genome sequencing and comparative genomic analysis of an ST398-MRSA-Vc isolate (NPREF115) from an RTE pork product in China. Using NPREF115 and 134 publicly available S. aureus genomes from diverse sources, we constructed a core genome phylogeny and conducted SNP and pangenome analyses, with a focus on MGEs. Results: Phylogenetic analysis revealed that our foodborne ST398-MRSA-Vc isolate clustered with human, Capra pyrenaica, bovine, and swine-derived ST398-MRSA-Vc isolates. SNP analysis indicated NPREF115 was most closely related to human clinical isolates (132 and 140 SNPs, respectively), consistent with shared ancestry rather than recent cross-host transmission. Genomic divergence was largely confined to MGEs, including SCCmec, prophages, genomic islands, and a chromosomally integrated Tn560 carrying the ant(9)-Ia-lsa(E)-lnu(B) multidrug resistance cluster. Notably, NPREF115 harbored a unique metabolic gene that may facilitate persistence in high-osmolarity food environments. Conclusions: The successful colonization of food by the ST398-MRSA-Vc isolate is likely associated with the acquisition of multiple MGEs harboring antimicrobial resistance genes. Transmission of ST398-MRSA-Vc between food, human, and livestock hosts was accompanied by changes in genes involved in metabolism. These findings underscore the importance of monitoring MGEs in genomic surveillance of foodborne MRSA. Full article

Background/Objectives: An effective inferior alveolar nerve block (IANB) is critical for mandibular third molar surgery, especially for novice student operators who face steep learning curves. This study compared the clinical efficacy and safety of 4% articaine, 2% lidocaine, and 2% mepivacaine in an undergraduate setting. Methods: A prospective observational study was conducted with 189 patients undergoing third molar surgery performed by dental students. Patients received either 4% articaine (n = 69), 2% lidocaine (n = 61), or 2% mepivacaine (n = 59). Anesthetic efficacy was evaluated using a two-stage assessment comprising Vincent’s sign (Stage 1) and profound surgical anesthesia (Stage 2). Intra- and postoperative pain, anesthetic volume, surgical duration, and postoperative complications were recorded and compared among anesthetic groups. Results: Baseline demographics, impaction patterns, and difficulty indices did not differ significantly among groups. Stage 2 profound success rate was significantly higher with articaine (76.8%) than with lidocaine (55.7%) and mepivacaine (61.0%) (p = 0.031). Articaine was also associated with a longer duration of anesthesia (261.7 vs. 164.6 and 192.6 min; p < 0.001), a lower total anesthetic volume (2.1 vs. 2.4 and 2.3 mL; p = 0.007), and significantly lower intraoperative pain scores (14.3 vs. 31.0 and 29.8 mm on the Heft–Parker VAS pain scale (HPS); p < 0.001). Postoperative pain through Day 7 and complication rates were comparable among anesthetics, with no serious adverse events reported. Conclusions: Within the limitations of this observational study, four percent articaine was associated with higher profound IANB success rates and lower intraoperative pain observed in this cohort. These observed associations with higher success and tissue diffusion properties may mitigate the impact of novice technical variability within this cohort. Full article

Microplastics are persistent contaminants in coastal wetlands, yet the mechanisms of their microbial transformation remain poorly understood. This study examined the interactions between a wetland sediment-derived microbial consortium and polyethylene terephthalate (PET) fibers over a 60-day incubation. After 60 days, the consortium caused a PET weight loss of 13.7 ± 0.9%, whereas the abiotic control showed a less than 2% loss. The water contact angle decreased from 77.5 ± 1.2° to 75.8 ± 0.4°, suggesting enhanced surface hydrophilicity. Multi-scale surface analyses (SEM, WCA, and FTIR) confirmed progressive microbial colonization, increased surface roughness, and enhanced hydrophilicity through microbially mediated modification. High-throughput 16S rRNA sequencing unveiled a distinct community succession; PET exerted selective pressure that reduced alpha-diversity while enriching specific functional taxa such as Acinetobacter and Pseudomonas. Moreover, isolation and co-culture assays confirmed the importance of synergistic microbial interactions in PET transformation, with co-culture of four representative isolates causing 9.2 ± 0.1% PET weight loss, compared with only 1.7–3.2% in monocultures. These findings underscore the intrinsic natural attenuation potential of wetland ecosystems and provide a critical scientific basis for developing nature-based management strategies. By identifying key functional taxa and PET-associated transformation pathways, this work supports the establishment of early-warning mechanisms to safeguard the ecological integrity and soil health of coastal World Natural Heritage sites like the Tiaozini Wetland. Full article

Antibiotic residues in water buffalo milk are a food-safety concern, yet depletion data are scarce. The purpose of this study was to characterize the depletion profiles of amoxicillin (AMOX) and its two major metabolites, amoxicilloic acid (AMA) and amoxicillin diketopiperazine-2′,5′-dione (2,5-DKP), in Anatolian water buffalo milk after a single intramuscular administration and to estimate a milk withdrawal time relative to the EU MRL. We tested the hypothesis that AMOX concentrations would decrease below the EU MRL over successive milkings and that AMA and 2,5-DKP would exhibit depletion kinetics distinct from the parent compound. Five lactating Anatolian water buffaloes received a single intramuscular injection of amoxicillin (15 milligrams per kilogram). Milk was collected at each milking (twice daily) for seven days and analyzed by liquid chromatography–tandem mass spectrometry with quantification limits below the European Union maximum residue limit for amoxicillin in milk (4 micrograms per kilogram). Amoxicillin peaked at the second milking (mean 13.65 micrograms per kilogram), mean concentrations fell below the maximum residue limit from the sixth milking, and they became non-quantifiable from the tenth milking onward. Two major metabolites, amoxicillinic acid and amoxicillin diketopiperazine-2′,5′-dione, peaked earlier (2,5-DKP Tmax 12 h) or at higher concentrations (AMA Cmax 32.64 µg/kg vs. AMOX 13.65 µg/kg) and remained detectable up to the thirteenth milking, with longer apparent terminal half-lives (32.0 and 52.8 h) than amoxicillin (23.5 h); the mixed-effects model confirmed different depletion rates among analytes (milking × analyte interaction p = 4.63 × 10⁻⁵). A log-linear withdrawal model applying the EMA 95/95 tolerance limit indicated that the first time point at which the upper tolerance limit fell below the EU MRL was 84.7 h after dosing; rounded up to the next 12 h milking interval, this corresponds to a reported withdrawal period of 96 h (≈8 milkings). These results provide species-specific residue kinetics for amoxicillin in Anatolian buffalo milk and support considering metabolites in monitoring and withdrawal-time decisions. Full article

Cast iron, whose structure simultaneously contains graphite precipitates in various forms, with controlled proportions of individual forms, has been named “Vari-Morph” (VM) cast iron by the authors. The authors have been researching the properties of such cast iron for many years, and the results are being published successively. This new type of cast iron, not included in national (Polish) or European standards, is intended as a material for special-purpose castings. These castings have unique requirements for a set of properties: physical, mechanical, and functional. VM cast iron is characterized by a set of properties that cannot be achieved when the graphite is uniform in shape. The desired properties of VM cast iron are achieved by controlling the morphology of graphite precipitates and the proportion of individual forms in the structure, rather than by changing the matrix. To quantitatively describe graphite precipitates, a proprietary method for determining the graphite shape indicator (f_K) was developed. Graphite precipitate analysis is performed by scanning a microscopic image of the metallographic specimen, and then using Tescan Imaging Software (Tescan ESSENCE™) Unified Control for Imaging and Analysis, each precipitate is described using surface metrology parameters. The final value of the graphite shape indicator (f_K) is calculated as a weighted average of all precipitates present in the analysis field. Empirical relationships between the f_K indicator and a selected group of physical, mechanical, and functional properties of VM cast iron were determined. Studies have demonstrated a very well-correlated relationship between the f_K indicator in VM cast iron and ultrasonic wave velocity (C_L). The relationship C_L = f(f_k) is characterized by a very high correlation coefficient of R > 0.90. In previous publications, the authors presented the relationships between the f_K indicator and physical properties such as thermal conductivity (λ), specific density (ρ), strength (R_m), elongation (A5), index quality (IQ), and functional properties such as low-cycle mechanical fatigue resistance (Z_c), thermal fatigue resistance (N), and cast iron tightness (H) as functions of the f_K index. The study concerned VM cast iron with a ferritic matrix. This work contains new empirical relationships that extend previous studies. The newly developed relationships replace the f_k shape indicator with the velocity of the ultrasonic wave determined in cast iron with a specific f_K indicator value. This resulted in a number of practical dependencies, including: λ = f(C_L); ρ = f(C_L); E_D = f(C_L); R_m = f(C_L); A5 = f(C_L); IQ = f(C_L); N = f(C_L); Z_c = f(C_L); H = f(C_L). These relationships allow us to measure the wave velocity in a Vari Morph iron casting (with various forms of graphite) and determine a number of characteristics and properties of the material/iron from which the casting was made. It is possible to assess the suitability of a casting with a specific structure for operation under selected conditions. Full article

This study is part of the FAIST research project—Agile, Intelligent, Sustainable and Technological Factory, coordinated by the Footwear Technology Centre of Portugal (CTCP), which aims to develop an innovative production process through the creation of a sustainable footwear model fully adapted to the user’s foot anatomy and personalized according to individual aesthetic preferences. Within this context, the need emerged to design an online platform with an interface capable of effectively addressing user needs throughout all stages of the personalization process, from the foot scanning to the aesthetic personalization of the model, while ensuring an efficient, intuitive, and pleasant navigation experience. Thus, this work aims to demonstrate how the design process of a footwear personalization platform, across its different phases, can contribute to the revitalization of the Portuguese footwear industry, as well as to describe its effectiveness, with the goal of being potentially adapted and implemented in similar contexts. The adopted methodology was based on the principles of Design Thinking, an approach centered on user needs. The development of the platform involved the creation of personas, the definition of the information architecture, the development of wireframes and workflows, and the execution of usability tests using the System Usability Scale (SUS). The results demonstrate a high success rate, validating the proposed solution with users and confirming the suitability of the applied methodologies. Full article

Chimeric antigen receptor (CAR)-T cell therapy has achieved remarkable clinical success in B cell malignancies. However, its efficacy in solid tumors remains limited, in part due to suboptimal expansion, persistence, and restrained effector function. Strategies that promote durable CAR-T cell fitness are therefore required to overcome these barriers. In this study, we generated HER2-CAR-T cells targeting human breast cancer cells and evaluated the impact of different cytokine supplementation strategies on CAR-T cell phenotype and function. We analyzed gene expression patterns and performed repetitive tumor killing assays to assess the ability of CAR-T cells expanded with IL-2 + IL-7 + IL-15 compared with IL-2 alone to maintain proliferation and cytotoxic function across multiple rounds of tumor cell exposure. Compared with IL-2 alone, supplementation with IL-7 and IL-15 significantly enhanced CAR-T cell expansion, preserved stem cell-like features prior to antigen encounter, and promoted superior proliferative capacity. Moreover, CAR-T cells cultured with IL-7+15 or IL-2+7+15 maintained sustained cytotoxicity and exhibited increased antitumor cytokine production during repeated tumor challenges. Notably, IL-7 and IL-15 supplementation induced a CD57⁺ CAR-T cell population that, unlike the immunosenescent CD57⁺ cells reported previously, retained full proliferative and cytotoxic capacity, with CD57 expression being dynamically downregulated upon antigen stimulation. Collectively, these findings demonstrate that incorporation of IL-7 and IL-15 into CAR-T cell manufacturing protocols substantially improves expansion, persistence, and effector function, supporting their use as a strategy to enhance CAR-T cell performance against solid tumors. Full article

Background: Atrial fibrillation (AF) frequently develops in patients with atrial flutter (AFL), even after successful cavotricuspid isthmus (CTI) ablation. Identifying simple clinical markers for early detection is crucial. Diagonal earlobe crease (ELC), also known as Frank’s sign, has been proposed as a marker of aging and cardiovascular risk. This study investigates the association between ELC and the risk of new-onset AF following CTI ablation in patients with AFL. Methods: We conducted a retrospective cohort study of 292 patients without a prior history of AF who underwent CTI ablation for typical AFL between 2015 and 2024. The presence of ELC was assessed at baseline CTI ablation. The primary outcome was the occurrence of new-onset AF during follow-up, stratified according to the presence of ELC. The median follow-up duration was 49 months, with a minimum follow-up of 6 months. Results: Among the 292 patients, 72 (24.7%) exhibited ELC. Patients with ELC were older (59 ± 11 years vs. 55 ± 14 years, p = 0.05). During the follow-up period, new-onset AF occurred in 31 patients with ELC (43.1%) and 65 patients without ELC (29.5%) (p = 0.03). Kaplan–Meier analysis demonstrated that the occurrence of AF was significantly higher in the ELC group than in the non-ELC group (log-rank test, p = 0.013). Multivariate analysis revealed that ELC was independently associated with an increased risk of AF (hazard ratio 1.67, 95% confidence interval 1.03–2.72, p = 0.039). Conclusions: The presence of ELC is associated with a higher risk of new-onset AF following CTI ablation in patients with AFL. ELC may serve as a simple, non-invasive clinical marker to identify patients who may benefit from closer rhythm surveillance after AFL ablation. Full article

Alluvial plains in the marginal zone of the monsoon system are sensitive to the climate–hydrology interaction. However, long term, high-resolution sedimentary records remain scarce in the Songnen Plain of Northeast China. This limited our understanding of the paleoclimate–paleohydrology coupling evolution over glacial–interglacial cycles. A 50.6 m continuous core was retrieved from the western Songnen Plain. The age–depth model and wavelet transform spectrum showed sedimentary continuity from ~885 ka B.P. (the late Early Pleistocene) to ~6 ka B.P. (the early Holocene), with no major hiatuses exceeding orbital resolution. Grain size analyses revealed 18 microfacies, which were synthesized into two major evolutionary cycles: a fan-delta front cycle (dominated by subaqueous mouth bars and distributary channels) and a fan-delta plain cycle (characterized by intertributary bays, floodplain lakes/swamps, and crevasse splays). The absence of pro-delta facies and the sediment succession record the oscillatory shrinkage of the Songnen paleolake. The pulsed enhancements of hydrodynamic energy, marked by grain size coarsening, coincide with major glacial–interglacial transitions (MIS 20/19, 18/17, 16/15, 14/13, 8/7, 6/5, 4/3, and 2/1), whereas fining grain sizes dominate warm interglacial periods (MIS 11, 9, 7, 5, 3, 1). These patterns are sensitive response of the alluvial plain to orbital-scale climate change. Cold–arid glacial background promoted vegetation loss and hydrological instability, and warm–humid interglacial background favored low-energy hydrological condition. This study demonstrates that the regional alluvial evolution was primarily controlled by global ice-volume fluctuations through variability of the East Asian summer monsoon. This study provides a reference for the muti-scale climate–hydrology coupling mechanism study in the northern marginal zone of EASM and highlights the importance of alluvial sediment succession in paleo-research. Full article

Introduction: The ureteral access sheath (UAS) is commonly used in retrograde intrarenal surgery (RIRS) to improve vision, lower intrarenal pressure (IRP), and facilitate access. However, concerns regarding ureteral injury remain. We conducted this study to evaluate the surgical efficacy and safety of a selective omission strategy for UAS use during RIRS in patients with small renal stones (<10 mm) or in cases where UAS placement is technically difficult. Materials and Methods: This retrospective study included consecutive patients who underwent single-surgeon RIRS at Changhua Christian Hospital between October 2020 and April 2023 for renal or upper ureteral stones. Sheathless RIRS was performed in patients with stones < 10 mm, or in whom insertion of a 10/12 Fr UAS was unsuccessful despite successful advancement of an 8 Fr semirigid ureteroscope, and when the surgeon estimated the procedure could be completed within 2 h. All procedures used a holmium laser with a 9 Fr or 7.5 Fr flexible ureteroscope. No patients were pre-stented, and all received postoperative double-J stenting. Results: Among 55 patients, 18 (32.7%) underwent sheathless RIRS and 37 (67.3%) underwent UAS-assisted RIRS. Stone size was significantly smaller in the sheathless group (12 mm vs. 17 mm, p = 0.001). The 3-month stone-free rate (SFR) was 66.7% in the sheathless group and 62.2% in the UAS group (p = 0.745). Operative time was similar between groups (77 vs. 85 min, p = 0.154), with no statistically significant differences in postoperative pain or length of hospital stay. In the UAS-assisted group, six patients developed febrile urinary tract infection, of whom two progressed to sepsis; all recovered after antibiotic therapy. No fever or sepsis occurred in the sheathless group. On multivariable analysis, lower calyceal stone location was independently associated with SFR, whereas UAS use was not. Conclusions: In a selected cohort (stones < 10 mm or difficult UAS insertion with an expected operative time < 2 h), sheathless RIRS was feasible and showed no statistically significant differences in SFR or perioperative outcomes compared with UAS-assisted RIRS. However, due to selection bias, stone-size imbalance, and limited statistical power, these findings should not be interpreted as procedural equivalence and require confirmation in adequately powered studies with stratified/adjusted analyses. Full article

Background: Peritonsillar abscess (PTA) is a common deep neck infection associated with significant healthcare resource utilization. Needle aspiration (NA) and/or incision & drainage (ID) are the most frequently used treatment modalities; however, their comparative clinical outcomes remain controversial. Methods: This retrospective cohort study included 185 adult patients hospitalized with a diagnosis of PTA between January 2018 and January 2026 at a tertiary care center. Patients aged ≥18 years who underwent NA or ID were included. Demographic characteristics, clinical findings, laboratory parameters at admission, treatment-related variables (type and number of interventions, drainage volume, adjunctive corticosteroid use), microbiological results, and length of hospital stay were recorded. Patients were categorized according to the drainage method performed (NA vs. ID). Results: A total of 185 patients were included, of whom 87 (47%) underwent NA and 98 (53%) underwent ID. There were no significant differences between groups in age, sex, or length of hospital stay (p > 0.05). CRP levels and lymphocyte counts were significantly higher in the NA group (p = 0.028 and p = 0.009, respectively), whereas drainage volume and number of interventions were significantly higher in the ID group (p < 0.001 and p = 0.005, respectively). Successful resolution on the first attempt was more frequent in the ID group (89.8% vs. 75.9%, p = 0.011), while steroid use was more common in the NA group (16.1% vs. 2.0%, p = 0.001). In multivariate analysis, drainage volume, first-attempt success, and corticosteroid use were independently associated with the choice of drainage method (p < 0.05). Conclusions: Both NA and ID are effective treatment options for PTA; however, ID was associated with higher first-attempt success and greater drainage volume. Indeed, NA was more frequently accompanied by steroid therapy. These findings suggest that treatment modality selection may influence short-term clinical outcomes in patients with PTA. Full article

In Australian education, the challenges and complexities of school leadership and the educational achievement of Aboriginal and Torres Strait Islander young people are frequently debated. Each has been given individual attention, but little attention to how school leadership and educational success for Aboriginal and Torres Strait Islander young people collectively interact. While there are increasing expectations of school leaders enabling the success of Aboriginal and Torres Strait Islander young people and Aboriginal and Torres Strait Islander perspectives embedded in schools, knowledge is required about the role of transformative leadership approaches that enable cultural responsiveness in the actions and practices of school leaders. Framed by Rigney’s Indigenist Research Theory (IRT) and Transformative Leadership Theory (TLT), this paper draws from a collective case study that gathers the lived experiences of school leadership from Aboriginal and Torres Strait Islander families, community, staff and school leader participants. The findings illuminate the actions and practices that school leadership enact to lead culturally responsive and inclusive school communities. The study argues the necessity of school leaders valuing and engaging with Aboriginal and Torres Strait Islander knowledges, perspectives and lived experiences as critical for advancing educational success for Aboriginal and Torres Strait Islander peoples, and education for all young people. Exploring the role of transformative leadership in the advancement of equity and excellence in Aboriginal and Torres Strait Islander education in Australia, this paper culminates in examining the implications for practice that also support the advancement of the United Nations Declaration on the Rights of Indigenous Peoples (UNDRIP), the United Nations Convention on the Rights of the Child (UNCRC), and the United Nations’ Sustainable Development Goal 4 (SDG 4) of equity, inclusion, and quality education and lifelong learning for all. Full article

Strategic alliances have assumed a pivotal role in the growth and competitiveness of organisations, especially in contexts of rapid technological change and high environmental complexity. Drawing on the Dynamic Capabilities View (DCV), this study aims to analyse the impact of strategic alliance management on technological, marketing, and new product development capabilities, considering the mediating role of dynamic capabilities. This research is based on a sample of 200 Portuguese firms, predominantly SMEs, using Structural Equation Modelling (SEM) to test a conceptual model composed of six hypotheses. The results demonstrate that effective alliance management is positively associated with dynamic capabilities, which in turn function as a pivotal mechanism for integrating and reconfiguring resources. Specifically, the findings reveal that these dynamic capabilities (exploration and exploitation) are fundamental to strengthening marketing and technological skills. Notably, technological capability did not yield a significant direct impact on new-product development, suggesting that in this service-intensive context, marketing capabilities and the overall orchestration of dynamic routines are more critical to innovation success. This research offers empirical evidence of how strategic alliances strengthen the competitiveness of SMEs in peripheral EU economies, highlighting that innovation stems from a configuration of integrative capabilities rather than technological assets alone. Full article