Search Results (1,611)

Background: Atrophy of the alveolar ridge in the posterior maxilla often requires sinus floor elevation prior to implant placement. Photobiomodulation using low-level laser therapy (LLLT) has been suggested as a supportive approach for bone healing, although data based on histological evaluation are still limited. Methods: This study presents histological and radiological secondary outcomes of a randomized clinical trial on bone regeneration after lateral window sinus augmentation. Twenty patients were allocated according to grafting material (allogeneic or xenogeneic) and the use of adjunctive LLLT. After 6 months, bone core biopsies were obtained at the time of implant placement and processed for histological analysis. Radiological bone gain was assessed using CBCT. Results: Bone gain was achieved in all groups, allowing implant placement in every case. Mean bone gain reached 7.53 ± 3.32 mm in LLLT-treated sites and 7.02 ± 2.00 mm in controls, with no statistically significant differences. Histological analysis confirmed trabecular bone formation across all groups. Mild inflammatory cell infiltrates were observed more frequently in LLLT-treated sites (p = 0.029), although this finding was not associated with impaired tissue organization or compromised healing. Conclusions: Both allogeneic and xenogeneic grafts showed good biocompatibility and supported effective bone regeneration after sinus augmentation. The addition of photobiomodulation did not demonstrate statistically significant clinical or radiological benefits within this exploratory cohort, but it may be associated with subtle differences in tissue remodeling. Full article

The sustainable conservation of linear industrial heritage corridors remains challenged by a limited understanding of their formation mechanisms and driving forces. Addressing this gap, this study develops a transferable analytical framework to explain the spatio-temporal evolution of such systems. Using Jiangsu Province (China) as a case study and a dataset of 344 industrial heritage sites, we apply an integrated spatial-analytical approach to examine distribution patterns and underlying drivers. The results reveal an evolving dual-axis spatial structure shaped by transportation networks and regional development dynamics, with railway density emerging as a key influencing factor. Furthermore, the interaction of infrastructural, demographic, and institutional variables highlights a synergistic mechanism underpinning corridor formation. Building on these findings, the study proposes a “corridor-as-process” framework, conceptualizing industrial heritage corridors as dynamic socio-spatial products of long-term interactions between institutions, networks, and economic activities. This perspective advances beyond static, descriptive approaches by offering a process-oriented and explanatory understanding of heritage systems. This study contributes to sustainability by providing a spatially explicit basis for adaptive reuse, vulnerability assessment, and differentiated conservation strategies, supporting the integration of heritage preservation within broader regional sustainability transitions. The proposed framework offers a transferable methodological reference for analyzing industrial heritage corridors in comparable global contexts. Full article

Levoglucosan (LG), a tracer of biomass-burning air pollution, was measured in PM₁₀ particulate matter during a year-long study at an urban background site in Zagreb, Croatia. It is known that the atmospheric lifetime of LG is not constant and undergoes degradation through reactions with hydroxyl radicals, ozone, photooxidation, etc. In this study, daily variations in LG were examined and evaluated in relation to NO₂, O₃, and meteorological conditions, including temperature, relative humidity, solar irradiance, UV index, and wind characteristics. The annual mean PM₁₀ concentration was 22 µg m⁻³, while LG average was 0.312 µg m⁻³, both exhibiting pronounced seasonal variability. Elevated LG levels occurred during winter and autumn, consistent with residential wood combustion and stable atmospheric conditions, whereas markedly lower concentrations were observed in spring and summer. Moderate correlations of LG with PM₁₀ and NO₂ indicate contributions from combustion sources, while weak wind speeds and limited dispersion favored pollutant accumulation. In contrast, significant negative relationships were found between LG and ozone, temperature, and UV index. The results revealed non-linear behavior and an exponential decrease in LG with increasing oxidant levels, suggesting pseudo–first-order degradation driven by enhanced photochemical activity and hydroxyl radical formation. These findings highlight the importance of considering both emission patterns and atmospheric processing when using levoglucosan as a tracer of biomass burning in urban environments. Full article

Background/Objectives: Exposure of cells to ionizing radiation induces isolated DNA lesions, including single-strand breaks, apurinic/apyrimidinic sites, and oxidized bases, as well as clustered damages of different complexity. The latter types of damage are difficult to repair, and the failure to process them accurately and efficiently is related to the induction of mutagenesis, genomic instability, cancer, and aging. Since various types of clustered lesions may occur simultaneously after radiation exposure, leading to a complex architecture of DNA damage, the study of the concomitant formation and the removal kinetics of clustered DNA damage is important to determine the mutagenic and, consequently, the carcinogenic potential of ionizing radiation. Methods: With the aim of capturing real-time coexisting lesion types and assessing the repair kinetics of clustered damages, the simultaneous determination of double-strand breaks, apurinic/apyrimidinic site clusters, and oxypurine clusters induced by γ-irradiation of Saccharomyces cerevisiae yeast cells was performed immediately after exposure and at time intervals during incubation in Liquid Holding Recovery conditions. Results: Ionizing radiation induced lethal and mutagenic events, leading to a dose-dependent linear increase in double-strand breaks, apurinic/apyrimidinic site clusters, and oxypurine clusters. The kinetic study showed that double-strand break frequencies declined during Liquid Holding Recovery, although a transient increase was detected at early time points. At 160 Gy, apurinic/apyrimidinic site clusters repair was evident, whereas at 400 Gy the frequency of damage increased before returning to the initial value at 24 h. In contrast, oxypurine clusters showed no net increase in repaired lesions over 24 h. Conclusions: The complex nature and topological characteristics of ionizing radiation-induced clustered DNA damage may influence lesion processing. Also, ionizing radiation may disrupt redox cellular homeostasis, leading to DNA damage and delayed effects. Full article

Radiotherapy is essential for treating head and neck cancer but frequently leads to radiation-induced fibrosis (RIF) in salivary glands (SGs). RIF develops through a cascade of radiation-triggered events, including DNA damage, excessive oxidative stress, and epithelial cell death. Persistent injury can cause cells to become senescent and release inflammatory signals, fueling chronic inflammation. These processes activate pathways, particularly TGF-β/SMAD, resulting in fibroblast activation, myofibroblast differentiation, and extracellular matrix accumulation. Potential treatments include drugs, mesenchymal stem/stromal cell (MSC) therapy, and gene-transfer approaches. In which, MSC therapy is particularly promising as MSCs can migrate to injured tissue and support epithelial regeneration. Yet progress is limited by the difficulty of expanding human acinar cells (ACs) in vitro. To address this gap, tunable alginate–gelatin–hyaluronic acid (AGHA) bioink hydrogels have emerged as a suitable system as gelatin provides adhesion sites for AC attachment and 3D organoid formation, alginate offers tunable mechanical support through ionic crosslinking, and hyaluronic acid contributes essential cues for cell adhesion, migration, and morphogenesis. The aim of this review is to synthesize current understanding of the mechanisms driving RIF, evaluate available therapeutic strategies, and highlight the role of AGHA in generating engineered SG constructs to test MSC therapies for RIF. Full article

Naphthalene and methylnaphthalene (Nap and MN) are the most abundant polycyclic aromatic hydrocarbons (PAHs) and are important precursors of secondary organic aerosol (SOA) in the atmosphere. 1.2-Phthalic acid (1,2-PhA) and 4-methylphthalic acid (4-MPhA) are usually treated as tracers of SOA from Nap and MN. However, the two tracers also have primary sources, and directly using the tracers to estimate SOA would lead to an overestimation. In this study, we conducted a one-year synchronous observation of the two-ring PAH SOA (SOA_2-rings) tracers at nine sites in the Pearl River Delta (PRD) region. We measured and filtered the suitable emission characteristics of SOA_2-rings tracers for biomass burning, coal combustion, industrial processes and vehicle exhaust sources. Then, we developed a method to distinguish 1,2-PhA and 4-MPhA from primary emissions and secondary formation. The average proportions of 1,2-PhA_pri and 4-MPhA_pri in 1,2-PhA and 4-MPhA were 26.7% and 29.2%, respectively. The direct application of measured 1,2-PhA for estimating SOA_2-rings would lead to an overestimation exceeding 30% in the PRD. Furthermore, we estimated SOA_2-rings using the separated 1,2-PhA_sec and 4-MPhA_sec by the tracer-based method. The average contribution of MN to SOA was around three times that of Nap. In addition, when combined with monocyclic aromatic SOA (SOA_1-ring) and biogenic SOA, the contributions of SOA_1-ring (21%) and SOA_2-rings (25%) to total SOA were comparable. SOA_2-rings was even the largest contributor to total SOA (~44%) in winter. This study revealed that whether to separate the SOA_2-rings tracers for primary emissions and secondary formation is essential in SOA estimation and highlighted that two-ring PAHs make a significant contribution to SOA in the PRD. Full article

Efficient charge transfer and effective separation of photo-generated charge carriers are pivotal to the photocatalytic process. In this study, a novel CoAl₂O₄@nitrogen-doped graphitic carbon (CoAl₂O₄@NGC) composite photocatalyst was fabricated via a stepwise hydrothermal method coupled with high-temperature calcination, and its photocatalytic performance for CO₂ reduction was systematically investigated. X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and photoelectrochemical measurements were employed to characterize the phase structure, microstructure, surface chemical state and photoelectrochemical properties of the catalyst. Spinel-structured CoAl₂O₄ nanoparticles were uniformly anchored on the NGC substrate, forming a well-integrated composite interface. XPS analysis confirmed the coexistence of Co²⁺/Co³⁺ mixed valence states in CoAl₂O₄ which provides abundant redox sites for CO₂ activation. Photocatalytic tests showed that CoAl₂O₄@NGC exhibits excellent catalytic activity and cycling stability, with CO and CH₄ yields of 27.88 μmol·g⁻¹·h⁻¹ and 23.90 μmol·g⁻¹·h⁻¹, respectively. The narrow bandgap (1.54 eV) enhances visible light absorption, while efficient electron-hole separation and reduced charge transfer resistance improve photocatalytic efficiency. Theoretical calculations further reveal that CoAl₂O₄@NGC lowers the adsorption free energy of CO₂ and the energy barrier for COOH formation, thus facilitating the photocatalytic CO₂ reduction. This work provides insights for the design of efficient and stable photocatalysts for CO₂ reduction and deepens the understanding of the synergistic catalytic mechanism in the spinel/nitrogen-doped carbon composite system. Full article

Background: Qualitative health research increasingly emphasizes relational and interactional processes in illness and caregiving; however, joint interview formats remain methodologically under-theorized. This article advances a relational and power-sensitive reconceptualization of the couple interview by conceptualizing the interview encounter itself as an interactional site in which caregiving relations become observable in real time rather than merely reported retrospectively. Methods: The article draws on seven in-home couple interviews with long-married older heterosexual couples in Germany, in which one partner provided long-term home-based care for the other. The analysis applies the Documentary Method to reconstruct jointly produced meanings, collective orientations, and the micro-interactional dynamics of the interview situation itself. Results: The analysis shows that couple interviews provide a distinctive methodological lens for studying dyadic caregiving by rendering co-narration, negotiated speaker roles, “we”-positioning, speaking-for-the-other, and embodied coordination analytically visible. Interactional asymmetries, interruptions, and situational role shifts thus emerge not only as challenges but as epistemic resources for reconstructing caregiving relationships and power dynamics. Based on this analysis, the article develops a three-part practice-oriented methodological toolkit comprising relational interviewing strategies, moderation practices, and systematic observation and documentation markers. Conclusions: By reframing the couple interview as an interactional event and specifying analytic markers and conduct strategies, this article makes an explicit methodological contribution to dyadic qualitative health research, particularly in sensitive later-life caregiving contexts. Full article

The synergistic effects of glucose (Glu) concentration and clay mineral type (kaolinite [Kao], montmorillonite [Mon]) on abiotic humification via the polyphenol-Maillard reaction remain poorly understood. To address these scientific challenges, a series of controlled, sterile batch experiments was conducted. Specifically, a glucose concentration gradient (0, 0.03, 0.06, 0.12, and 0.24 mol/L) was established; Kao and Mon were separately introduced as mineral catalysts; and the Maillard reaction was facilitated in the presence of catechol and glycine under strictly abiotic conditions to preclude any potential biological interference. Comprehensive analyses were performed on the reaction products—namely, the supernatant and the dark-brown residue generated during the reaction process. These analyses included: the E₄/E₆ ratio and total organic carbon (TOC) content of the supernatant; the carbon-based ratio of humic-like acid to fulvic-like acid (C_HLA/C_FLA); and the structural characteristics of humic-like acid (HLA) isolated from the dark-brown residue. Results showed dynamic E₄/E₆ ratio and TOC changes in the supernatant were accurately described by the Logistic function. Kao favored soluble organic C accumulation and enhanced retention of early-stage, low-molecular-weight intermediates in the dark-brown residue, while Mon promoted humic-like substances (HLS) polymerization and aromatic condensation. FTIR spectroscopy analysis identified optimal Glu thresholds for maximal HLS formation—0.03 mol/L for Kao and 0.06 mol/L for Mon—indicating non-linear, rather than monotonic, dependence on Glu dosage. Comparative pre- and post-reaction Fourier-transform infrared (FTIR) spectroscopy further demonstrated that Mon, owing to Mg–OH octahedral sites arising from isomorphic substitution, formed more stable Cat chelates than Kao. These chelates effectively stabilized surface-bound hydroxyl-associated water molecules and modulated the electron cloud distribution around Si–O bonds. Collectively, this study clarified the dual regulatory role of Glu concentration and clay mineral identity in abiotic humification pathways, advanced mechanistic understanding of clay mineral-mediated polyphenol-Maillard reactions, and established a scientific foundation for optimizing humification efficiency in both engineered and natural systems. Full article

Bluetongue virus (BTV), with a genome of ten double-stranded RNA segments (S1–S10), is an emerging animal pathogen causing major economic losses in livestock worldwide. BTV replication involves RNA-RNA and RNA–protein interactions, with RNA-binding proteins, VP6 and NS2 playing key roles in genome assembly and RNA packaging. To explore the dynamics of RNA segment interactions and the roles of VP6 and NS2 in RNA complex formation, we used RNA fluorescence in situ hybridization chain reaction (HCR), along with site-specific mutagenesis and reverse genetics. We found that RNA segments interact sequentially, from the smallest (S10) to the largest (S1), forming a single complex that includes the entire genome. This process is independent of VP6 or NS2, although NS2 enhances the assembly of larger segments. Additionally, we show that VP6 binds to +ssRNAs before their incorporation into viral assembly factories (inclusion bodies/VIBs). These findings reveal that RNA-RNA interactions, rather than primary replicase proteins, govern the sorting and recruitment of genome segments. Our data offer new insights into BTV RNA packaging, showing that genome segments destined for packaging and dsRNA synthesis are segregated through complex formation, distinct from +ssRNAs used in protein synthesis, including those encoding the replicase complex. Full article

Produced-water (PW) management in the Permian Basin faces tightening injection constraints, induced seismicity concerns, and volatile saltwater disposal (SWD) costs. At the same time, chemistry-rich PW contains dissolved constituents (e.g., Li, B, and Sr) that may be valorized if SWD recovery performance and market conditions support favorable techno-economics. Here, we develop an integrated decision-support framework that couples (i) chemistry-informed surrogate models for unit process performance (recovery, effluent quality, and energy/chemical intensity) with (ii) a network-based allocation model that routes PW from sources through pretreatment, optional treatment and mineral-recovery modules (e.g., desalination and direct lithium extraction), and end-use nodes (beneficial reuse, hydraulic fracturing reuse, mineral recovery/valorization, or Class II disposal). This is a screening-level demonstration using publicly available chemistry percentiles and representative pilot-reported performance windows; it is not a site-specific facility design or a bankable TEA for a particular operator. The optimization is posed as a tri-objective problem—to maximize expected net present value, minimize SWD, and minimize an injection-risk indicator R—subject to mass balance, capacity, quality, and regulatory constraints. Uncertainty in commodity prices, recovery fractions, and operating costs is propagated via Monte Carlo scenario sampling, yielding PARETO-efficient portfolios that quantify trade-offs between profitability and risk mitigation. Using the PW chemistry percentiles reported by the Texas Produced Water Consortium for the Delaware and Midland Basins, we derive screening-level break-even lithium concentrations and illustrate how lithium-carbonate-equivalent price and recovery govern the extent to which mineral revenue can offset SWD expenditures. Comparative brine benchmarks (Smackover Formation and Salton Sea geothermal systems) contextualize the Permian’s generally lower-Li PW and highlight transferability of the workflow across brine types. The proposed framework provides a transparent, extensible basis for design matrix planning under evolving injection limits, enabling risk-aware PW management strategies that reduce disposal dependence while improving water resilience. Full article

The bottom water of the Shizhouji Formation tight sandstone reservoir in the Tazhong Shun 9 well area is developed. General fracturing faces the problem of excessive extension of hydraulic fractures and easy communication with water layers. A true triaxial fracturing physical simulation experiment was conducted on the sandstone and mudstone outcrops of the same layer to explore the expansion laws of hydraulic fractures in the tight sandstone reservoir and consider the influence of mudstone interlayers, horizontal stress difference, fracturing fluid flow rate, and viscosity. The mechanism of multi-cluster fractures/artificial fractures penetrating through the layers was revealed. The research results show that the existence of mudstone interlayers greatly increases the complexity of fractures, from 1.88 to 2.96, an increase of 57%. When there is a mudstone interlayer in the rock, the fracturing process is prone to open weak planes, hindering the expansion of hydraulic fractures. The hydraulic fractures of Sample No. 4 were cut off four times and penetrated through the layers once. The larger the flow rate, the greater the complexity of hydraulic fractures, and the easier the fractures penetrate through the layers. The fractures with a large flow rate (200 mL/min) were cut off three times, and the stress difference was larger, the hydraulic fractures tended to be simple, and the penetration through the layers was zero times at a high-level stress difference (18 MPa); the greater the viscosity, the greater the fracture pressure, and the complexity of fractures first increased and then decreased; the greater the viscosity, the more easily the hydraulic fractures penetrate through the layers, with low viscosity cutting off three times, medium viscosity cutting off four times, and high viscosity cutting off five times. Therefore, considering the limitation requirements of the on-site fracturing on the extension of fracture height, it is recommended that the on-site fracturing construction flow rate be 6 m³/min, and the fracturing fluid viscosity be 10 mPa·s. Full article

This paper argues that digital identity in AI-mediated environments has become a central mechanism through which contemporary societies organise recognition, participation, and belonging. Digital identity is no longer simply a technical representation of the individual. It is produced through infrastructural processes of classification, ranking, and credibility signalling that determine who becomes visible, who is treated as legitimate, and who is able to participate meaningfully in social and civic life. The paper develops a conceptual framework that treats AI-driven platforms as social infrastructures rather than neutral intermediaries. It shows how identity is inferred through data-driven systems rather than negotiated through social interaction, how recognition is operationalised through visibility and credibility metrics rather than ethical judgement, and how participation becomes conditional on algorithmic allocation of attention rather than guaranteed by access alone. Visibility is identified as the key conversion point through which inferred identity becomes social consequence. Drawing on interdisciplinary literature, the analysis demonstrates that misrecognition, exclusion, and inequality in platform environments are not primarily the result of isolated error or intentional bias. They are patterned outcomes of ordinary optimisation processes that distribute legitimacy and opportunity unevenly across social groups. These dynamics reshape group formation, harden social boundaries, and concentrate risk among populations that are already more vulnerable to misrecognition and reduced contestability. The paper concludes that governing digital identity is a societal challenge rather than a purely technical one. As platforms increasingly perform institutional functions without equivalent accountability, digital identity governance becomes a critical site of social ordering. Addressing this challenge requires public standards for how visibility, recognition, and participation are allocated, meaningful avenues for contestation, and protections against the normalisation of stratified belonging in AI-mediated societies. Full article

Atmospheric aerosols modulate Earth’s radiation balance through direct effects and through their role as cloud condensation nuclei (CCN), contributing to variability in near-surface temperature (NST). Galactic cosmic rays (GCRs) further influence aerosol–cloud interactions by enhancing particle formation and growth, but combined aerosol optical depth (AOD)–GCR effects on NST remain poorly constrained across climates. Using satellite and reanalysis data, we examine joint influences on NST anomalies at three neutron-monitoring stations, Oulu, Newark, and Hermanus, during 2000–2022. The sites share similar geomagnetic cutoffs but contrasting climates, enabling separation of ionization from geomagnetic shielding. Multiple linear regression (MLR) captures AOD effects and their modulation by GCR flux. Adding an interaction term (AOD × GCR) improves fit, raising adjusted R² from 0.22→0.31 (Oulu), 0.37→0.52 (Newark), and 0.69→0.78 (Hermanus). ECMWF reanalysis shows hydrophilic organic matter aerosol (OMA) dominates (0.19, 0.29, 0.41 µg kg⁻¹ at Oulu, Newark and Hermanus), with sulphate elevated at Oulu/Newark and coarse sea salt at Hermanus. Elevated OMA and sulphate at Oulu/Newark imply GCR-enhanced fine CCN and cooling, whereas humid, sea-salt-rich Hermanus favors ion-mediated growth of larger hygroscopic particles that increase longwave trapping and warming. Findings provide site-specific evidence that GCR ionization modulates aerosol processes and contributes to regional NST variability, informing improved parameterizations in climate models. Full article

The adaptive nature of bacteria and their increasing resistance to conventional therapies demand alternative strategies to effectively control wound infections. At the wound site, dynamic biological processes are easily disrupted by microbial colonization, compromising normal healing. In this study, Zn-based nanoparticles composed of zinc oxide (ZnO) and zinc phosphate (Zn₃(PO₄)₂) were synthesized via a green route using coconut milk and coconut water as biological media. Although ZnO formation via zinc hydroxide intermediates was initially targeted, structural analyses revealed a multiphase Zn-based system resulting from interactions between Zn²⁺ ions and naturally occurring phosphate species in the coconut-derived sources. The resulting material was incorporated into sodium alginate/poly(vinyl alcohol) hydrogel dressings, further enhanced with spirulina and aronia powders. Physicochemical characterization (XRD, SEM, EDS, FTIR), along with swelling and degradation studies, confirmed structural stability and appropriate hydrogel behavior. Antimicrobial testing against Staphylococcus aureus and Escherichia coli demonstrated a dominant antibiofilm effect of the Zn-based nanoparticles, while botanical additives exhibited moderate, time-dependent activity. Biological evaluation demonstrated good cytocompatibility toward human keratinocytes and murine macrophages, with botanical additives mitigating mild nanoparticle-induced cellular responses. Full article