Search Results (17)

Quercus faginea Lam., a winter-deciduous oak native to the Iberian Peninsula, typically grows under a Mediterranean climate. To identify the main drivers influencing radial wood increment, we analyzed the climatic signals in tree-ring width and wood anatomical traits using increment cores. Winter conditions influenced both latewood width and earlywood vessel size in the first row. Latewood was positively correlated with precipitation and temperature, with the long-term positive effect of winter water supply supported by SPEI. In contrast, vessel size showed negative correlations, also reflecting a long-term negative effect of winter precipitation. Consequently, conditions that enhanced latewood width and overall tree-ring growth appear to be associated with the formation of smaller earlywood vessels. Although ample winter precipitation replenishes soil water reserves and supports prolonged wood formation, it may also induce anaerobic soil conditions that promote root fermentation, depleting carbohydrates needed for cell turgor and expansion, and ultimately regulating earlywood vessel size. This physiological decoupling may help explain the lack of a significant correlation between latewood width and earlywood vessel size, underscoring their independent responses to environmental influences. Our findings highlighted the complex interplay between various climatic conditions affecting Q. faginea, with implications for understanding its adaptive capacity in changing climates. Full article

Climate change has affected forest ecosystems across the world over the past century. However, its impact is particularly high in the Himalayas due to increasing temperatures, extreme precipitation events, and regional droughts. In this context, a review of the current stage of research was deemed necessary to understand the adaptation of a key conifer species to climate variability in the Central Himalayas. Hence, we conducted a systematic review of published peer-reviewed journal articles addressing the growth performance of Abies spectabilis (D. Don) Spach in the Central Himalayas. From this review, three main patterns of climate response have emerged: a positive correlation of radial tree growth with temperature of the current and previous growing seasons, tree growth limitation by winter temperature, and by temperature or moisture in the pre-monsoon season. Overall, results indicate an elevation-dependent temperature sensitivity, a crucial role of moisture availability, and seasonal shifts in climate–growth relationships, reflecting the species’ adaptability to changing climate conditions. Our review revealed that studies on elevation-dependent adaptation of wood anatomical traits by A. spectabilis are still rare. The tree-ring growth of this species shows a complex response to climate variability, with increasing as well as decreasing growth trends across its distribution range. Full article

Wood functional traits provide information for the management and sustainable use of species. This study evaluated the wood specific gravity (SG) and nine anatomical characteristics of wood in six sections (three levels of stem height and three orders of the branch) in six species of bioeconomic importance for the Colombian Amazon region: Jacaranda copaia, Virola elongata, Virola peruviana, Cedrelinga cateniformis, Erisma uncinatum, and Cabari macrocarpa. The results showed that low- and medium-SG species have branches with equal or greater SG than the stem. In this group, Erisma uncinatum and Virola peruviana showed no differences between their sections. In contrast, for high-SG species such as Cabari macrocarpa, the relationship was inverse. Fiber thickness correlated mainly with SG, with no differences between sections, except in Cabari macrocarpa. Fiber length decreased in all species in the stem-to-branch direction. The other characteristics varied, suggesting an optimization in the effort of water transport along the tree. These findings infer a potential sustainable use of branches of tree species with low biomechanical variation, such as Erisma uncinatum and Virola peruviana. They also demonstrate the hydraulic and mechanical adaptability of these species, which is relevant when facing climate change scenarios. Full article

Olive wood is used in a niche economic context but is attracting growing interest. In this study, the wood anatomy of Olea europaea L. belonging to two cultivars cultivated in the Plain of Gioia Tauro in Calabria (RC) is qualitatively described. Wood samples were obtained along the diameter of wood slices to investigate any anatomical differences between the inner and outer zones of the stem. The microscopic slides were investigated using an optical microscope. The anatomical characteristics observed were compared with existing literature data. The two cultivars show parenchyma rays arranged not only in one to two rows (typical of this species), but also in three rows. Furthermore, in both cultivars, the presence of starch deposits in procumbent parenchyma cells was observed. The Ottobratica cultivar seems to have more starch than the Sinopolese one, but given the high variability of olive wood, further quantitative analysis is needed to determine whether these differences are statistically valid and due to the different cultivars. This work can contribute to a better understanding of the Olea europaea L. species and to a better technical valorisation of its wood. Full article

This study investigates the anatomical characteristics and growth patterns of Salix arctica and Salix herbacea, two prevalent dwarf shrub species in Iceland, to understand their responses to environmental changes. We employed optical and scanning electron microscopy methods and quantitative wood anatomy to analyze the stem and root structures of studied species. Additionally, we developed chronologies and assessed the climatic response of both the stem and root parts for both species. Our results reveal significant differences between the two species, with S. arctica exhibiting larger vessels and fibers compared to S. herbacea, both in stem and root. The growth trends differ between the species: S. arctica shows an overall increase, while S. herbacea exhibits a consistent decline. Both species’ individual parts generally follow these trends, though a recent decline has been observed in the last few years. Climatic responses also differ, highlighting specific climatic parameters influencing each species. S. arctica responds positively to warmer temperatures, while S. herbacea reacts positively to increased precipitation but struggles with rising temperatures, highlighting its role as a drought indicator species. Soil erosion driven by volcanic materials and extreme climates significantly impacts shrub growth, causing rapid changes in growth ring widths and vessel sizes. Understanding these impacts is vital for improving sampling methods in polar environments. This study highlights the importance of integrated wood anatomical studies in comprehending the ecological consequences of climate change on Arctic shrubs, providing new insights into the complexity of shrub expansion both below and above ground. Full article

Norway spruce (Picea abies) forests in temperate zones are already reacting to short-term extreme summer heatwaves, threatening the vitality of trees and forest productivity, and can even lead to local and regional dieback events. Examining quantitative wood anatomy can provide helpful information in terms of understanding the physiology mechanisms and related responses of conifer trees to local environmental interactions in relation to tracheid adaptive capacity. This study analysed the tracheid functional anatomical traits (FATs) plasticity of six young Norway spruce trees growing in two mesic research plots with high annual precipitation (~43%) and air temperature differences during 2010–2017. The research plots are located in the sub-mountainous (Rájec Němčice) and mountainous (Bílý Kříž) belts of the Moravia region, Czech Republic. Vapour pressure deficit and cell wall reinforcement index (CWRI) were shown to be the most representative environmental parameters as proxies of dry conditions. Tracheid FATs indicated latewood phenological plasticity sensitivity, with more pronounced variability in the warmer and drier plots. Latewood tracheids of Norway spruce trees grown in the RAJ formed significantly thicker cell walls than BK during the studied period. The observed differences between the two research plots indicate additional support for tracheid cells’ hydraulic safety against cavitation and potential traces of adaptive acclimatization response. Full article

We compared climate–growth relationships (1956–2013) of two natural pedunculate oak (Quercus robur L.) stands with different water-holding capacities growing at the species distribution limit of the Mediterranean Region in NW Iberia. For this, tree-ring chronologies of earlywood vessel size (separating the first row from the other vessels) and latewood width were obtained. Earlywood traits were coupled to conditions during dormancy, whereby an elevated winter temperature appears to induce a high consumption of carbohydrates, resulting in smaller vessels. This effect was reinforced by waterlogging at the wettest site, whose correlation to winter precipitation was strongly negative. Soil water regimes caused differences between vessel rows, since all earlywood vessels were controlled by winter conditions at the wettest site, but only the first row at the driest one; radial increment was related to water availability during the previous rather than the current season. This confirms our initial hypothesis that oak trees near their southern distribution boundary adopt a conservative strategy, prioritizing reserve storage under limiting conditions during the growing period. We believe that wood formation is highly dependent on the balance between the previous accumulation of carbohydrates and their consumption to maintain both respiration during dormancy and early spring growth. Full article

Quantitative wood anatomy (QWA) is widely used to resolve a fundamental problem of tree responses to past, ongoing and forecasted climate changes. Potentially, QWA data can be considered as a new proxy source for long-term climate reconstruction with higher temporal resolution than traditional dendroclimatic data. In this paper, we considered a tracheidogram as a set of two interconnected variables describing the dynamics of seasonal variability in the radial cell size and cell wall thickness in conifer trees. We used 1386 cell profiles (tracheidograms) obtained for seven Scots pine (Pinus sylvestris) trees growing in the cold semiarid conditions of Southern Siberia over the years 1813–2018. We developed a “deviation tracheidogram” approach for adequately describing the traits of tree-ring formation in different climate conditions over a long-term time span. Based on the NbClust approach and K-means method, the deviation tracheidograms were reliably split into four clusters (classes) with clear bio-ecological interpretations (from the most favorable growth conditions to worse ones) over the years 1813–2018. It has been shown that the obtained classes of tracheidograms can be directly associated with different levels of water deficit, for both the current and previous growing seasons. The tracheidogram cluster reconstruction shows that the entire 19th century was characterized by considerable water deficit, which has not been revealed by the climate-sensitive tree-ring chronology of the study site. Therefore, the proposed research offers new perspectives for better understanding how tree radial growth responds to changing seasonal climate and a new independent proxy for developing long-term detailed climatic reconstructions through the detailed analysis of long-term archives of QWA data for different conifer species and various forest ecosystems in future research. Full article

Wood encodes environmental information that can be recovered through the study of tree-ring width and wood anatomical variables such as lumen area or cell-wall thickness. Anatomical variables often provide a stronger hydroclimate signal than tree-ring width, but they show a low tree-to-tree coherence. We investigate the sources of variation in tree-ring width, lumen area, and cell-wall thickness in three pine species inhabiting sites with contrasting climate conditions: Pinus lumholtzii in wet-summer northern Mexico, and Pinus halepensis and Pinus sylvestris in dry-summer north-eastern Spain. We quantified the amount of variance of these three variables explained by spring and summer water balance and how it varied among trees. Wood anatomical variables accounted for a larger inter-individual variability than tree-ring width data. Anatomical traits responded to hydroclimate more individualistically than tree-ring width. This individualistic response represents an important issue in long-term studies on wood anatomical characteristics. We emphasized the degree of variation among individuals of the same population, which has far-reaching implications for understanding tree species’ responses to climate change. Dendroclimatic and wood anatomical studies should focus on trees rather than on the mean population series. Full article

The arrangement of vessels and their grouping is unique in most tree species. When observing tiny, microscopic samples of wood, the arrangement of the wood vessels forms a characteristic and repetitive pattern, which is largely determined by the tree species, but it is also influenced by the site conditions as well as its location in the tree. The present study is part of a project aimed at applying computer vision and computer recognition methods to present a more general and comprehensive group classification of wood vessels. Quantitative descriptions of the grouping of vessels, as a rule, have so far been used mainly to reveal characteristic deviations from the typical structure of wood, for example, due to extreme site conditions. Therefore, they are applicable but not sufficient for the present study and need in-depth revision. A classification of vessels is presented depending on their mutual position, and more precisely, the groups of adjacent vessels are determined using quantitative methods. The quantitative indicators used for this purpose are based on the diameter and other quantitative indicators of the vessels’ arrangements. The proposed classification, although based on a long-known classification scheme in structural wood science, allows for the more precise definition of the classes of a grouping of adjacent vessels in a cross-section as a necessary step towards the wider use of the methods of machine recognition of wood. Full article

The application of quantitative wood anatomy (QWA) in dendroclimatic analysis offers deep insight into the climatic effect on tree-ring formation, which is crucial in understanding the forests’ response to climate change. However, interrelations between tree-ring traits should be accounted to separate climatic signals recorded during subsequent stages of cell differentiation. The study was conducted in the South Siberian alpine timberline on Pinus sibirica Du Tour, a species considered unpromising in dendroclimatology. Relationships between tree-ring width, cell number N, mean and maximum values of radial diameter D, and cell wall thickness (CWT) were quantified to obtain indexed anatomical chronologies. Exponential functions with saturation D(N) and CWT(N) were proposed, which explained 14–69% and 3–61% of their variability, respectively. Indexation unabated significance of the climatic signals but separated them within a season. Analysis of pointer years and climatic extremes revealed predominantly long-term climatogenic changes of P. sibirica radial growth and QWA and allowed to obtain QWA-based 11-year filtered reconstructions of vegetative season climatic characteristics (R²_adj = 0.32–0.66). The revealed prevalence of low-frequency climatic reactions is probably explained by a strategy of slow accumulation and utilization of resources implemented by P. sibirica. It makes this species’ QWA a promising proxy for decadal climatic variations in various intra-seasonal timeframes. Full article

More than 60% of tree phytomass is concentrated in stem wood, which is the result of periodic activity of the cambium. Nevertheless, there are few attempts to quantitatively describe cambium dynamics. In this study, we develop a state-of-the-art band model of cambium development, based on the kinetic heterogeneity of the cambial zone and the connectivity of the cell structure. The model describes seasonal cambium development based on an exponential function under climate forcing which can be effectively used to estimate the seasonal cell production for individual trees. It was shown that the model is able to simulate different cell production for fast-, middle- and slow-growing trees under the same climate forcing. Based on actual measurements of cell production for two contrasted trees, the model effectively reconstructed long-term cell production variability (up to 75% of explained variance) of both tree-ring characteristics over the period 1937−2012. The new model significantly simplifies the assessment of seasonal cell production for individual trees of a studied forest stand and allows the entire range of individual absolute variability in the ring formation of any tree in the stand to be quantified, which can lead to a better understanding of the anatomy of xylem formation, a key component of the carbon cycle. Full article

The need to identify wood by its anatomical features requires a detailed analysis of all the elements that make it up. This is a significant problem of structural wood science, the most general and complete solution of which is yet to be sought. In recent years, increasing attention has been paid to the use of computer vision methods to automate processes such as the detection, identification, and classification of different tissues and different tree species. The more successful use of these methods in wood anatomy requires a more precise and comprehensive definition of the anatomical elements, according to their geometric and topological characteristics. In this article, we conduct a detailed analysis of the limits of variation of the location and grouping of vessels in the observed microscopic samples. The present development offers criteria and quantitative indicators for defining the terms shape, location, and group of wood tissues. It is proposed to differentiate the quantitative indicators of the vessels depending on their geometric and topological characteristics. Thus, with the help of computer vision technics, it will be possible to establish topological characteristics of wood vessels, the extraction of which would be used to develop an algorithm for the automatic classification of tree species. Full article

Research Highlights: Our results provide novel perspectives on the effectiveness and collapse of compensatory mechanisms of tracheid development of Norway spruce during intra-seasonal drought and the environmental control of intra-annual density fluctuations. Background and Objectives: This study aimed to compare and integrate complementary methods for investigating intra-annual wood formation dynamics to gain a better understanding of the endogenous and environmental control of tree-ring development and the impact of anticipated climatic changes on forest growth and productivity. Materials and Methods: We performed an integrated analysis of xylogenesis observations, quantitative wood anatomy, and point-dendrometer measurements of Norway spruce (Picea abies (L.) Karst.) trees growing along an elevational gradient in South-western Germany during a growing season with an anomalous dry June followed by an extraordinary humid July. Results: Strong endogenous control of tree-ring formation was suggested at the highest elevation where the decreasing rates of tracheid enlargement and wall thickening during drought were effectively compensated by increased cell differentiation duration. A shift to environmental control of tree-ring formation during drought was indicated at the lowest elevation, where we detected absence of compensatory mechanisms, eventually stimulating the formation of an intra-annual density fluctuation. Transient drought stress in June also led to bimodal patterns and decreasing daily rates of stem radial displacement, radial xylem growth, and woody biomass production. Comparing xylogenesis data with dendrometer measurements showed ambivalent results and it appears that, with decreasing daily rates of radial xylem growth, the signal-to-noise ratio in dendrometer time series between growth and fluctuations of tree water status becomes increasingly detrimental. Conclusions: Our study provides new perspectives into the complex interplay between rates and durations of tracheid development during dry-wet cycles, and, thereby, contributes to an improved and mechanistic understanding of the environmental control of wood formation processes, leading to the formation of intra-annual density fluctuations in tree-rings of Norway spruce. Full article

Research Highlights: This study emphasized the importance of multi-parameter analyses along ecological gradients for a more holistic understanding of the complex mechanism of tree-ring formation. Background and Objectives: The analysis of climatic signals from cell anatomical features measured along series of tree-rings provides mechanistic details on how environmental drivers rule tree-ring formation. However, the processes of cell development might not be independent, limiting the interpretation of the cell-based climatic signal. In this study, we investigated the variability, intercorrelations and climatic drivers of wood anatomical parameters, resulting from consequent cell developmental processes. Materials and Methods: The study was performed on thin cross-sections from wood cores sampled at ~1.3 m stem height from mature trees of Pinus sylvestris L. growing at five sampling sites along an ecological gradient from cold and wet to hot and dry within continental Southern Siberia. Tracheid number per radial file, their diameters and wall thicknesses were measured along the radial direction from microphotographs for five trees per site. These parameters were then averaged at each site for earlywood and latewood over the last 50 tree rings to build site chronologies. Their correlations among themselves and with 21-day moving climatic series were calculated. Results: Our findings showed that wood formation was not simply the result of environmentally driven independent subprocesses of cell division, enlargement and wall deposition. These processes appear to be interconnected within each zone of the ring, as well as between earlywood and latewood. However, earlywood parameters tend to have more distinctive climatic responses and lower intercorrelations. On the other hand, there are clear indications that the mechanisms of cell division and enlargement share similar climatic drivers and are more sensitive to water limitation than the process of wall deposition. Conclusions: Indications were provided that (i) earlywood formation left a legacy on latewood formation, (ii) cell division and enlargement shared more similar drivers between each other than with wall deposition, and (iii) the mechanism of cell division and enlargement along the gradient switch from water to heat limitations at different thresholds than wall deposition. Full article