Search Results (12)

Background: Steroidal glucocorticoid and gestagenic drugs and cold plasma-treated solutions (PTSs) are known to exert anti-inflammatory effects by influencing the production of a number of cytokines. The aim of this work was to test their independent and combined effects exerted on the production of cytokines IL-1, IL-6, TNF-α, TGF-β, and IL-10 and reactive oxygen and nitrogen species (RONS) by leukocytes in women of a reproductive age and postmenopausal women. Methods: ELISA and chemiluminescence methods were used for this purpose. Results: PTS reduced IL-6 and RONS production by 50% and increased IL-10 production 2-fold in postmenopausal women, and it reduced IL-6 production by 80% and RONS production by 50% in women of reproductive age. When PTS and steroid hormonal drugs are used together, there is a general suppression of cytokine and oxidant activity. Conclusions: PTS reduces the production of inflammatory factors by leukocytes and stimulates the production of anti-inflammatory factors, more so in postmenopausal women. Progestins showed greater suppression of pro-inflammatory cytokine and RONS formation and stimulation of anti-inflammatory cytokines for women of reproductive age and dexamethasone showed such results for postmenopausal women. Full article

Addressing the challenges associated with the highly exothermic nature of CO₂ methanation, there is considerable interest in innovative catalyst designs on structural metallic supports. One promising solution in this regard involves thin films containing cobalt oxide within a carbon matrix, fabricated using the cold plasma deposition method (PECVD). The objective of this study was to search for a relationship between the molecular structure, nanostructure, and electronic structure of such films and their catalytic activity. The investigations employed various techniques, including X-ray photoelectron spectroscopy (XPS), ultraviolet photoelectron spectroscopy (UPS), X-ray diffraction (XRD), UV-VIS absorption, and catalytic tests in the CO₂ methanation process. Three types of films were tested: untreated as-deposited (ad-CoO), thermally post-treated (TT-CoO), and argon plasma post-treated (PT-CoO) films. Among these, TT-CoO exhibited the most favorable catalytic properties, demonstrating a CO₂ conversion rate of 83%, CH₄ selectivity of 98% at 400 °C, and stability during the catalytic process. This superior performance was attributed to the formation of nanoscale heterojunctions in the TT-CoO film, where p-type CoO nanocrystallites interacted with the n-type carbon matrix. This work provides compelling evidence highlighting the key role of nanoscale heterojunctions in shaping the properties of nanocatalysts in thermal catalysis. These findings suggest promising prospects for designing new catalytic systems by manipulating interactions at the nanoscale. Full article

The anti-cancer properties of plasma-treated solutions (PTS) and their interaction with drugs are one of the most popular topics in modern plasma medicine. Our research involved comparing the effects of four physiological saline solutions (0.9% NaCl, Ringer’s solution, Hank’s Balanced Salt Solution, Hank’s Balanced Salt Solution with amino acids added in concentrations observed in the human blood) treated with cold atmospheric plasma and studying the combined cytotoxic effect of PTS with doxorubicin and medroxyprogesterone acetate (MPA). Analysis of the effect of the studied agents on the formation of radicals in the incubation medium, the vitality of K562 myeloid leukaemia cells, and the processes of autophagy and apoptosis in them revealed two key findings. The first is that when using PTS and doxorubicin-containing PTS, autophagy is the predominant process in cancer cells. The second is that combining PTS with MPA enhances apoptotic processes. It was hypothesised that while autophagy is stimulated by the accumulation of reactive oxygen species in the cell, apoptosis is stimulated through specific cell progesterone receptors. Full article

Electroimpedance spectroscopy technology can be used to accelerate the healing of complete trees and estimate the plant condition after grafting. This approach will allow sorting out low-vigor plants at the early stages of their development to save time and resources. Still, in some cases, the use of electrical impedance spectroscopy can be difficult due to the complexity of the equipment and special measurement conditions. In this paper, we attempt to overcome this limitation by suggesting a compact device developed in-house that is usable even in the field. Pear (Pyrus communis L.) Otradnenskaya was used as the object of this study. We assessed the treatment effect of the scion–rootstock interface with cold atmospheric plasma (CAP) and plasma-treated solution (PTS) on the survival of the grafts. The dependence of the impedance of the complete grafted tree on the signal frequency and the length of the measuring section was analyzed. It is shown that the treatment of the scion and rootstock with CAP and PTS promotes the fusion of scion and rootstock. The impedance value in the control was on average 24–35% higher than in plants treated with CAP and PTS, which indicates a better healing process of the grafting site. This can be an indication of better quality of the planting material which can be obtained much earlier than with the conventional approach (monitoring the plants in a nursery). Full article

A garden plant grafting technique enhanced by cold plasma (CAP) and plasma-treated solutions (PTS) is described for the first time. It has been shown that CAP created by a dielectric barrier discharge (DBD) and PTS makes it possible to increase the growth of Pyrus communis L. by 35–44%, and the diameter of the root collar by 10–28%. In this case, the electrical resistivity of the graft decreased by 20–48%, which indicated the formation of a more developed vascular system at the rootstock–scion interface. The characteristics of DBD CAP and PTS are described in detail. Full article

Presently, plasma-treated solutions (PTS) are widely introduced into medicine. Plasma-activated solutions contain various reactive forms of oxygen and nitrogen which provide the desired biological effects. Yet it remains unclear exactly which components of the treated solution are the most important and what the difference is between the plasma-treated solution and a chemically prepared solution composed of the same components. In this work, we show that the chemically prepared mixture of nitrites, nitrates, and hydrogen peroxide with concentrations similar to the plasma-treated solution exerts a fundamentally different effect on a cell culture. The chemically prepared solution has higher cyto- and genotoxicity and causes necrosis, while under the action of the plasma-treated solution, apoptotic processes develop in the cells slowly. Full article

Chronic lymphocytic leukemia (CLL) is the most common blood malignancy with multiple therapeutic challenges. Cold physical plasma has been considered a promising approach in cancer therapy in recent years. In this study, we aimed to evaluate the cytotoxic effect of cold plasma or plasma-treated solutions (PTS) on hematologic parameters in the whole blood of CLL patients. The mean red blood cell count, white blood cell (WBC) count, platelet and hemoglobin counts, and peripheral blood smear images did not significantly differ between treated and untreated samples in either CLL or healthy individuals. However, both direct plasma and indirect PTS treatment increased lipid peroxidation and RNS deposition in the whole blood of CLL patients and in healthy subjects. In addition, the metabolic activity of WBCs was decreased with 120 s of cold plasma or PTS treatment after 24 h and 48 h. However, cold plasma and PTS treatment did not affect the prothrombin time, partial thromboplastin time, nor hemolysis in either CLL patients or in healthy individuals. The present study identifies the components of cold plasma to reach the blood without disturbing the basic parameters important in hematology, confirming the idea that the effect of cold plasma may not be limited to solid tumors and possibly extends to hematological disorders. Further cellular and molecular studies are needed to determine which cells in CLL patients are targeted by cold plasma or PTS. Full article

Cold Atmospheric Plasma (CAP) is an ionized gas near room temperature. Its anti-tumor effect can be transmitted either by direct treatment or mediated by a plasma-treated solution (PTS), such as treated standard cell culture medium, which contains different amino acids, inorganic salts, vitamins and other substances. Despite extensive research, the active components in PTS and its molecular or cellular mechanisms are not yet fully understood. The purpose of this study was the measurement of the reactive species in PTS and their effect on tumor cells using different plasma modes and treatment durations. The PTS analysis yielded mode- and dose-dependent differences in the production of reactive oxygen and nitrogen species (RONS), and in the decomposition and modification of the amino acids Tyrosine (Tyr) and Tryptophan (Trp). The Trp metabolites Formylkynurenine (FKyn) and Kynurenine (Kyn) were produced in PTS with the 4 kHz (oxygen) mode, inducing apoptosis in Mel Im melanoma cells. Nitrated derivatives of Trp and Tyr were formed in the 8 kHz (nitrogen) mode, elevating the p16 mRNA expression and senescence-associated ß-Galactosidase staining. In conclusion, the plasma mode has a strong impact on the composition of the active components in PTS and affects its anti-tumor mechanism. These findings are of decisive importance for the development of plasma devices and the effectiveness of tumor treatment. Full article

Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (T_maxROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased T_maxROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing. Full article

Plasma Treated Water Solutions (PTWS) recently emerged as a novel tool for the generation of Reactive Oxygen and Nitrogen Species (ROS and RNS) in liquids. The presence of ROS with a strong oxidative power, like hydrogen peroxide (H₂O₂), has been proposed as the main effector for the cancer-killing properties of PTWS. A protective role has been postulated for RNS, with nitric oxide (NO) being involved in the activation of antioxidant responses and cell survival. However, recent evidences proved that NO-derivatives in proper mixtures with ROS in PTWS could enhance rather than reduce the selectivity of PTWS-induced cancer cell death through the inhibition of specific antioxidant cancer defenses. In this paper we discuss the formation of RNS in different liquids with a Dielectric Barrier Discharge (DBD), to show that NO is absent in PTWS of complex composition like plasma treated (PT)-cell culture media used for in vitro experiments, as well as its supposed protective role. Nitrite anions (NO₂^-) instead, present in our PTWS, were found to improve the selective death of Saos2 cancer cells compared to EA.hy926 cells by decreasing the cytotoxic threshold of H₂O₂ to non-toxic values for the endothelial cell line. Full article

Cold physical plasma is a partially ionized gas generating various reactive oxygen and nitrogen species (ROS/RNS) simultaneously. ROS/RNS have therapeutic effects when applied to cells and tissues either directly from the plasma or via exposure to solutions that have been treated beforehand using plasma processes. This review addresses the challenges and opportunities of plasma-treated solutions (PTSs) for cancer treatment. These PTSs include plasma-treated cell culture media in experimental research as well as clinically approved solutions such as saline and Ringer’s lactate, which, in principle, already qualify for testing in therapeutic settings. Several types of cancers were found to succumb to the toxic action of PTSs, suggesting a broad mechanism of action based on the tumor-toxic activity of ROS/RNS stored in these solutions. Moreover, it is indicated that the PTS has immuno-stimulatory properties. Two different routes of application are currently envisaged in the clinical setting. One is direct injection into the bulk tumor, and the other is lavage in patients suffering from peritoneal carcinomatosis adjuvant to standard chemotherapy. While many promising results have been achieved so far, several obstacles, such as the standardized generation of large volumes of sterile PTS, remain to be addressed. Full article

Melittin (MEL), a small peptide component of bee venom, has been reported to exhibit anti-cancer effects in vitro and in vivo. However, its clinical applicability is disputed because of its non-specific cytotoxicity and haemolytic activity in high treatment doses. Plasma-treated phosphate buffered saline solution (PT-PBS), a solution rich in reactive oxygen and nitrogen species (RONS) can disrupt the cell membrane integrity and induce cancer cell death through oxidative stress-mediated pathways. Thus, PT-PBS could be used in combination with MEL to facilitate its access into cancer cells and to reduce the required therapeutic dose. The aim of our study is to determine the reduction of the effective dose of MEL required to eliminate cancer cells by its combination with PT-PBS. For this purpose, we have optimised the MEL threshold concentration and tested the combined treatment of MEL and PT-PBS on A375 melanoma and MCF7 breast cancer cells, using in vitro, in ovo and in silico approaches. We investigated the cytotoxic effect of MEL and PT-PBS alone and in combination to reveal their synergistic cytological effects. To support the in vitro and in ovo experiments, we showed by computer simulations that plasma-induced oxidation of the phospholipid bilayer leads to a decrease of the free energy barrier for translocation of MEL in comparison with the non-oxidized bilayer, which also suggests a synergistic effect of MEL with plasma induced oxidation. Overall, our findings suggest that MEL in combination with PT-PBS can be a promising combinational therapy to circumvent the non-specific toxicity of MEL, which may help for clinical applicability in the future. Full article