Search Results (7)

Natural and synthetic colorants present in food can modulate hemostasis, which includes the coagulation process and blood platelet activation. Some colorants have cardioprotective activity as well. However, the effect of genipin (a natural blue colorant) and synthetic blue colorants (including patent blue V and brilliant blue FCF) on hemostasis is not clear. In this study, we aimed to investigate the effects of three blue colorants—genipin, patent blue V, and brilliant blue FCF—on selected parameters of hemostasis in vitro. The anti- or pro-coagulant potential was assessed in human plasma by measuring the following coagulation times: thrombin time (TT), prothrombin time (PT), and activated partial thromboplastin time (APTT). Moreover, we used the Total Thrombus formation Analysis System (T-TAS, PL-chip) to evaluate the anti-platelet potential of the colorants in whole blood. We also measured their effect on the adhesion of washed blood platelets to fibrinogen and collagen. Lastly, the cytotoxicity of the colorants against blood platelets was assessed based on the activity of extracellular lactate dehydrogenase (LDH). We observed that genipin (at all concentrations (1–200 µM)) did not have a significant effect on the coagulation times (PT, APTT, and TT). However, genipin at the highest concentration (200 µM) and patent blue V at the concentrations of 1 and 10 µM significantly prolonged the time of occlusion measured using the T-TAS, which demonstrated their anti-platelet activity. We also observed that genipin decreased the adhesion of platelets to fibrinogen and collagen. Only patent blue V and brilliant blue FCF significantly shortened the APTT (at the concentration of 10 µM) and TT (at concentrations of 1 and 10 µM), demonstrating pro-coagulant activity. These synthetic blue colorants also modulated the process of human blood platelet adhesion, stimulating the adhesion to fibrinogen and inhibiting the adhesion to collagen. The results demonstrate that genipin is not toxic. In addition, because of its ability to reduce blood platelet activation, genipin holds promise as a novel and valuable agent that improves the health of the cardiovascular system and reduces the risk of cardiovascular diseases. However, the mechanism of its anti-platelet activity remains unclear and requires further studies. Its in vivo activity and interaction with various anti-coagulant and anti-thrombotic drugs, including aspirin and its derivatives, should be examined as well. Full article

This work focuses on the adsorptive removal of patent blue V (PBV) dye from aqueous solution by Zn/Al layered double hydroxide in fresh (LDH) and calcined (CLDH) forms. The material was synthesized via coprecipitation and samples were characterized by XRD, FTIR and TGA-DTA. Dye retention was evaluated under different experimental conditions of contact time, pH, adsorbent dosage, temperature and initial dye concentration. Experimental results show that highest adsorption capacity occurred at acidic medium. Kinetics data were properly fitted with the pseudo-second-order model. Equilibrium data were best correlated to Langmuir model with maximum monolayer adsorption capacities of 185.40 and 344.37 mg/g, respectively, for LDH and CLDH. The process was endothermic and spontaneous in nature. Based on the preliminary study, full factorial experimental design (2⁴) was used for the optimization of the effect of solution pH, adsorbent dose, initial dye concentration and the calcination. Thus, the optimal conditions to reach high equilibrium adsorption capacity were achieved at pH of 5, adsorbent dosage of 0.1 g/L, and initial dye concentration of 15 mg/L by CLDH. Full article

Oral vaccination of dogs against rabies has the potential to achieve mass coverage and thus deplete the virus of its most important reservoir host species. There is, however, no established non-invasive method to evaluate vaccine release in the oral cavity, following bait ingestion. In this study, two pre-selected marker methods in conjunction with their acceptance were assessed in local Thai dogs. Shelter dogs (n = 47) were offered one of four randomized bait formulations; bait type A-, containing Green S (E142) in a fructose solution; type B-, containing Patent Blue V (E131) in a fructose solution; type C-, containing the medium used for delivery of oral rabies vaccine in baits commercially produced; and type D-, containing denatonium benzoate, which was to serve as the negative control, due to its perceived bitterness. Patent Blue V was found to possess overall stronger dyeing capacities compared to Green S. Furthermore, there was no significant difference in the acceptance or bait handling of Patent Blue V baits compared to those containing the oral rabies vaccine medium alone, suggesting the potential use of this dye as a surrogate for rabies vaccine when testing newly developed bait formats. Full article

Choices of blue food colourants are extremely limited, with only two options in the USA, synthetic blue no. 1 and no. 2, and a third available in Europe, patent blue V. The food industry is investing heavily in finding naturally derived replacements, with limited success to date. Here, we review the complex and multifold mechanisms whereby blue pigmentation by anthocyanins is achieved in nature. Our aim is to explain how structure determines the functionality of anthocyanin pigments, particularly their colour and their stability. Where possible, we describe the impact of progressive decorations on colour and stability, drawn from extensive but diverse physico-chemical studies. We also consider briefly how this understanding could be harnessed to develop blue food colourants on the basis of the understanding of how anthocyanins create blues in nature. Full article

Biochar prepared from Sterculia alata fruit shell showed a better performance for dye removal than the biomass from Sterculia alata fruit shell. The important process parameters—namely the pH, the amount of biochar, the initial dye concentration and the contact time—were optimized in order to maximize dye removal using biochar of Sterculia alata fruit shell as the bio-sorbent. The results from this study showed that the maximum adsorption of dye on the biochar was obtained at a biochar dosage of 40 g/L, at a contact time of 5 h, and an initial dye concentration of 500 mg/L (pH 2.0; temperature 30 ± 5 °C). The increase in the rate adsorption with temperature and the scanning electron microscopic (SEM) images indicated the possibility of multilayer type adsorption which was confirmed by better fit of the Freundlich adsorption isotherm with the experimental data as compared to the Langmuir isotherm. The values n and R² in the Freundlich isotherm were found to be 4.55 and 0.97, respectively. The maximum adsorption capacity was found to be 11.36 mg/g. The value of n > 1 indicated physical nature of the adsorption process. The first and second order kinetics were tested, and it was observed that the adsorption process followed the first-order kinetics (R² = 0.911). Full article

A smart grid employs information and communications technology to improve the efficiency, reliability, economics, and sustainability of electricity production and distribution. The convergent and complex nature of a smart grid and the multifarious connection between its individual technology components, as well as competition between private companies, which will exert substantial influences on the future smart grid business, make a strategic approach necessary from the beginning of research and development (R&D) planning with collaborations among various research groups and from national, industry, company, and detailed technological levels. However, the strategic, technological, business environmental, and regulatory barriers between various stakeholders with collaborative or sometimes conflictive interests need to be clarified for a breakthrough in the smart grid field. A strategic R&D planning process was developed in this study to accomplish the complicated tasks, which comprises five steps: (i) background research of smart grid industry; (ii) selection of R&D target; (iii) societal, technological, economical, environmental, and political (STEEP) analysis to obtain a macro-level perspective and insight for achieving the selected R&D target; (iv) patent analysis to explore capabilities of the R&D target and to select the entry direction for smart grid industry; and, (v) nine windows and scenario planning analyses to develop a method and process in establishing a future strategic R&D plan. This R&D planning process was further applied to the case of a Korean company holding technological capabilities in the sustainable smart grid domain, as well as in the sustainable electric vehicle charging system, a global consumer market of smart grid. Four plausible scenarios were produced by varying key change agents for the results of this process, such as technology and growth rates, policies and government subsidies, and system standards of the smart grid charging system: Scenario 1, ‘The Stabilized Settlement of the Smart Grid Industry’; Scenario 2, ‘The Short-lived Blue Ocean of the Smart Grid Industry’; Scenario 3, ‘The Questionable Market of the Smart Grid’; and, Scenario 4, ‘The Stalemate of the Smart Grid Industry’. The R&D plan suggestions were arranged for each scenario and detailed ways to cope with dissonant situations were also implied for the company. In sum, in this case study, a future strategic R&D plan was suggested in regard to the electric vehicle charging technology business, which includes smart grid communication system, battery charging duration, service infrastructure, public charge station system, platform and module, wireless charging, data management system, and electric system solution. The strategic R&D planning process of this study can be applicable in various technologies and business fields, because of no inherent dependency on particular subject, like electric vehicle charging technology based on smart grid. Full article

The prospective utilization of bael shell (Aegle marmelos) as an agro-waste for the production of biochar was investigated along with its characterization and application for the abatement of hazardous aqueous Patent Blue (PB) dye solution. The sorptive removal of PB on bael shell biochar (BSB) was investigated under the following operational conditions: (pH, 2.7–10.4; biochar dosage, 2–12 g/L; and contact time, 0–60 min). The removal efficiency of PB by BSB in a batch adsorption experiment was 74% (pH 2.7 and 30 ± 5 °C). In addition, a clear relationship between the adsorption and pH of the solution was noticed and the proposed material recorded a maximum sorption capacity of 3.7 mg/g at a pH of 2.7. The adsorption of PB onto BSB was best explained by the pseudo-second order kinetic model (R² = 0.972), thereby asserting the predominant role of chemisorption. The active role of multiple surface-active functionalities present on BSB during PB sorption was elucidated with the help of Freundlich isotherm (R² = 0.968). Further, an adsorption mechanism was proposed by utilizing Fourier transform infrared spectroscopy (FTIR). Full article