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Keywords = outdoor/indoor farming

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14 pages, 738 KiB  
Article
Assessment of Pupillometry Across Different Commercial Systems of Laying Hens to Validate Its Potential as an Objective Indicator of Welfare
by Elyse Mosco, David Kilroy and Arun H. S. Kumar
Poultry 2025, 4(3), 31; https://doi.org/10.3390/poultry4030031 - 15 Jul 2025
Viewed by 268
Abstract
Background: Reliable and non-invasive methods for assessing welfare in poultry are essential for improving evidence-based welfare monitoring and advancing management practices in commercial production systems. The iris-to-pupil (IP) ratio, previously validated by our group in primates and cattle, reflects autonomic nervous system [...] Read more.
Background: Reliable and non-invasive methods for assessing welfare in poultry are essential for improving evidence-based welfare monitoring and advancing management practices in commercial production systems. The iris-to-pupil (IP) ratio, previously validated by our group in primates and cattle, reflects autonomic nervous system balance and may serve as a physiological indicator of stress in laying hens. This study evaluated the utility of the IP ratio under field conditions across diverse commercial layer housing systems. Materials and Methods: In total, 296 laying hens (Lohmann Brown, n = 269; White Leghorn, n = 27) were studied across four locations in Canada housed under different systems: Guelph (indoor; pen), Spring Island (outdoor and scratch; organic), Ottawa (outdoor, indoor and scratch; free-range), and Toronto (outdoor and hobby; free-range). High-resolution photographs of the eye were taken under ambient lighting. Light intensity was measured using the light meter app. The IP ratio was calculated using NIH ImageJ software (Version 1.54p). Statistical analysis included one-way ANOVA and linear regression using GraphPad Prism (Version 5). Results: Birds housed outdoors had the highest IP ratios, followed by those in scratch systems, while indoor and pen-housed birds had the lowest IP ratios (p < 0.001). Subgroup analyses of birds in Ottawa and Spring Island farms confirmed significantly higher IP ratios in outdoor environments compared to indoor and scratch systems (p < 0.001). The IP ratio correlated weakly with ambient light intensity (r2 = 0.25) and age (r2 = 0.05), indicating minimal influence of these variables. Although White Leghorn hens showed lower IP ratios than Lohmann Browns, this difference was confounded by housing type; all White Leghorns were housed in pens. Thus, housing system but not breed was the primary driver of IP variation. Conclusions: The IP ratio is a robust, non-invasive physiological marker of welfare assessment in laying hens, sensitive to housing environment but minimally influenced by light or age. Its potential for integration with digital imaging technologies supports its use in scalable welfare assessment protocols. Full article
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12 pages, 579 KiB  
Article
Autonomous Farmers Use of Complementary and Alternative Veterinary Medicines in Pasture-Based Dairy Goat Systems
by Jacques Cabaret and Vincent Lictevout
Animals 2025, 15(11), 1627; https://doi.org/10.3390/ani15111627 - 31 May 2025
Viewed by 436
Abstract
Consumers expect ruminants to graze outdoors and flocks to be of a reasonable size. Dairy goats can be kept outdoors (natural) or indoors. There are few pasture-grazed-system goat farms. The farms we studied in the centre-west of France were either organic or conventional, [...] Read more.
Consumers expect ruminants to graze outdoors and flocks to be of a reasonable size. Dairy goats can be kept outdoors (natural) or indoors. There are few pasture-grazed-system goat farms. The farms we studied in the centre-west of France were either organic or conventional, and all used pastures and tended to meet consumer demand for naturalness. We obtained information through semi-directed interviews. Dairy goats are susceptible to gastrointestinal infections when using pastures and this was one of the main health problems mentioned by organic farmers. There are a very limited number of medicines available for lactating dairy goats and farmers can use complementary and alternative veterinary medicine (CAVM), where they are completely autonomous in their choice. The use of CAVM has also been driven by organic labels and protected designation of origin (PDO) regulations for goat cheese. Homeopathy, phytotherapy, and aromatherapy were used for various health problems on almost all the farms surveyed. Herd size and farm area were negatively associated with the complex use of CAVM, possibly due to the workload on larger farms. Some CAVMs were used more in relation to the management of farms: aromatherapy in organic farms and homeopathy in cheese-making farms. The farmers with higher levels of education were more likely to employ phytotherapy. The farmers were autonomous in the choice of CAVM and did not rely on the advice of veterinarians. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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26 pages, 14214 KiB  
Article
Stereo Visual Odometry and Real-Time Appearance-Based SLAM for Mapping and Localization in Indoor and Outdoor Orchard Environments
by Imran Hussain, Xiongzhe Han and Jong-Woo Ha
Agriculture 2025, 15(8), 872; https://doi.org/10.3390/agriculture15080872 - 16 Apr 2025
Viewed by 1365
Abstract
Agricultural robots can mitigate labor shortages and advance precision farming. However, the dense vegetation canopies and uneven terrain in orchard environments reduce the reliability of traditional GPS-based localization, thereby reducing navigation accuracy and making autonomous navigation challenging. Moreover, inefficient path planning and an [...] Read more.
Agricultural robots can mitigate labor shortages and advance precision farming. However, the dense vegetation canopies and uneven terrain in orchard environments reduce the reliability of traditional GPS-based localization, thereby reducing navigation accuracy and making autonomous navigation challenging. Moreover, inefficient path planning and an increased risk of collisions affect the robot’s ability to perform tasks such as fruit harvesting, spraying, and monitoring. To address these limitations, this study integrated stereo visual odometry with real-time appearance-based mapping (RTAB-Map)-based simultaneous localization and mapping (SLAM) to improve mapping and localization in both indoor and outdoor orchard settings. The proposed system leverages stereo image pairs for precise depth estimation while utilizing RTAB-Map’s graph-based SLAM framework with loop-closure detection to ensure global map consistency. In addition, an incorporated inertial measurement unit (IMU) enhances pose estimation, thereby improving localization accuracy. Substantial improvements in both mapping and localization performance over the traditional approach were demonstrated, with an average error of 0.018 m against the ground truth for outdoor mapping and a consistent average error of 0.03 m for indoor trails with a 20.7% reduction in visual odometry trajectory deviation compared to traditional methods. Localization performance remained robust across diverse conditions, with a low RMSE of 0.207 m. Our approach provides critical insights into developing more reliable autonomous navigation systems for agricultural robots. Full article
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20 pages, 3356 KiB  
Article
Analyzing the Risk of Short-Term Losses in Free-Range Egg Production Using Commercial Data
by Yusuf Adewale Adejola, Terence Zimazile Sibanda, Isabelle Ruhnke, Johan Boshoff, Saluna Pokhrel and Mitchell Welch
Agriculture 2025, 15(7), 743; https://doi.org/10.3390/agriculture15070743 - 31 Mar 2025
Viewed by 654
Abstract
Free-range egg production plays a key role in the global food system, and current market trends suggest that consumer demand for free-range eggs will continue to rise. Free-range egg production is susceptible to a wide range of factors, including climatic conditions, management practices, [...] Read more.
Free-range egg production plays a key role in the global food system, and current market trends suggest that consumer demand for free-range eggs will continue to rise. Free-range egg production is susceptible to a wide range of factors, including climatic conditions, management practices, and disease presence. These factors can cause variability in the laying rate of a flock over time, leading to fluctuations in egg production. The main purpose of this study was to investigate the risk of short-term free-range egg production losses using data derived from a combination of sensing technologies and management activities. Production and environmental data were collected from a commercial farm comprising seven flocks of laying hens. The variables studied included laying rate, feed intake, water intake, solar radiation, humidity, precipitation, and indoor/outdoor temperature. These were processed into a set of aggregate features calculated across a 14-day moving window. Generalized estimating equations were used to analyze the association between the derived production and environmental features and the probability of a short-term drop in egg production, expressed through deviations in the laying rate on the day immediately following the data window. Odds ratios were used to express the relative risk of a production drop by comparing the features for window periods where production drops occur to the window periods where production drops did not occur. The results demonstrated that a range of data features based on the laying rate, feed intake, water intake, and indoor/outdoor temperatures all had significant associations with the odds of a production drop. Key findings from the study show that an increase in feed intake and laying rate measured across the 14-day data window were correlated with a lower risk of a sudden drop in egg production. Conversely, a low mean indoor temperature (x < 16.1 °C group), measured through environmental sensing data, was correlated with a higher risk of a sudden drop in egg production. This study quantifies the link between data features derived from production and environmental monitoring and egg production issues, thereby providing useful insights on the most important data items captured through day-to-day monitoring, which can be used for proactive management. Further research should be carried out to investigate how technologies such as machine learning and analytics platforms can be applied for the task of forecasting production interruptions using the data features explored in this study. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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14 pages, 1013 KiB  
Article
Integrating Indoor Hibernation into the Italian Outdoor Snail Farming System: A Potential Solution for Colder Climates
by Ramona Ștef, Dan Manea, Anișoara Aurelia Ienciu, Emilian Onișan, Dragoș Vasile Nica and Alin Cărăbeț
Animals 2025, 15(7), 914; https://doi.org/10.3390/ani15070914 - 22 Mar 2025
Viewed by 548
Abstract
(1) Background: Hibernation in Lutrasil-covered enclosures led to elevated mortality rates for Cornu aspersum on Romanian farms. This two-year study evaluated the feasibility of adapting indoor hibernation technology to the Italian outdoor snail farming (IOSF) system as a solution for overwintering mature C. [...] Read more.
(1) Background: Hibernation in Lutrasil-covered enclosures led to elevated mortality rates for Cornu aspersum on Romanian farms. This two-year study evaluated the feasibility of adapting indoor hibernation technology to the Italian outdoor snail farming (IOSF) system as a solution for overwintering mature C. aspersum snails. (2) Methods: Body weight, survival rates, and mortality factors during key hibernation phases (purging and overwintering) were monitored in three commercial snail farms using different hibernation scenarios. Labor efficiency was evaluated, comparing the use of micro shelters vs. hand-picking for snail collection. We analyzed post-purging/post-hibernation weight loss and mortality in hibernation spaces with and without proper thermal insulation. Mortality causes were also investigated. (3) Results: Using micro shelters significantly reduced labor time for snail collection. Weight loss during purging, but not during overwintering, were similar between groups. Post-hibernation survival ranged from 69% to 79% for comparable hibernation durations. Significantly lower survival was associated with significantly higher weight loss and using hibernation spaces with minimal thermal insulation. Predators exerted a very limited effect on post-hibernation survival, with most death appearing to be related to environmental causes. (4) Integrating indoor hibernation into the IOSF system is a promising strategy for the successful rearing of C. aspersum in colder climates. Full article
(This article belongs to the Special Issue Environmental Enrichment in Farm Animals)
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36 pages, 12089 KiB  
Review
Sensing Technologies for Outdoor/Indoor Farming
by Luwei Wang, Mengyao Xiao, Xinge Guo, Yanqin Yang, Zixuan Zhang and Chengkuo Lee
Biosensors 2024, 14(12), 629; https://doi.org/10.3390/bios14120629 - 19 Dec 2024
Cited by 5 | Viewed by 2041
Abstract
To face the increasing requirement for grains as the global population continues to grow, improving both crop yield and quality has become essential. Plant health directly impacts crop quality and yield, making the development of plant health-monitoring technologies essential. Variable sensing technologies for [...] Read more.
To face the increasing requirement for grains as the global population continues to grow, improving both crop yield and quality has become essential. Plant health directly impacts crop quality and yield, making the development of plant health-monitoring technologies essential. Variable sensing technologies for outdoor/indoor farming based on different working principles have emerged as important tools for monitoring plants and their microclimates. These technologies can detect factors such as plant water content, volatile organic compounds (VOCs), and hormones released by plants, as well as environmental conditions like humidity, temperature, wind speed, and light intensity. To achieve comprehensive plant health monitoring for multidimensional assessment, multimodal sensors have been developed. Non-invasive monitoring approaches are also gaining attention, leveraging biocompatible and flexible sensors for plant monitoring without interference with its natural growth. Furthermore, wireless data transmission is crucial for real-time monitoring and efficient farm management. Reliable power supplies for these systems are vital to ensure continuous operation. By combining wearable sensors with intelligent data analysis and remote monitoring, modern agriculture can achieve refined management, resource optimization, and sustainable production, offering innovative solutions to global food security and environmental challenges. Full article
(This article belongs to the Special Issue Wearable Sensors for Plant Health Monitoring)
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29 pages, 11272 KiB  
Article
Hardware Development and Evaluation of Multihop Cluster-Based Agricultural IoT Based on Bluetooth Low-Energy and LoRa Communication Technologies
by Emmanuel Effah, George Ghartey, Joshua Kweku Aidoo and Ousmane Thiare
Sensors 2024, 24(18), 6113; https://doi.org/10.3390/s24186113 - 21 Sep 2024
Cited by 4 | Viewed by 2425
Abstract
In this paper, we present the development and evaluation of a contextually relevant, cost-effective, multihop cluster-based agricultural Internet of Things (MCA-IoT) network. This network utilizes commercial off-the-shelf (COTS) Bluetooth Low-Energy (BLE) and LoRa communication technologies, along with the Raspberry Pi 3 Model B+ [...] Read more.
In this paper, we present the development and evaluation of a contextually relevant, cost-effective, multihop cluster-based agricultural Internet of Things (MCA-IoT) network. This network utilizes commercial off-the-shelf (COTS) Bluetooth Low-Energy (BLE) and LoRa communication technologies, along with the Raspberry Pi 3 Model B+ (RPi 3 B+), to address the challenges of climate change-induced global food insecurity in smart farming applications. Employing the lean engineering design approach, we initially implemented a centralized cluster-based agricultural IoT (CA-IoT) hardware testbed incorporating BLE, RPi 3 B+, STEMMA soil moisture sensors, UM25 m, and LoPy low-power Wi-Fi modules. This system was subsequently adapted and refined to assess the performance of the MCA-IoT network. This study offers a comprehensive reference on the novel, location-independent MCA-IoT technology, including detailed design and deployment insights for the agricultural IoT (Agri-IoT) community. The proposed solution demonstrated favorable performance in indoor and outdoor environments, particularly in water-stressed regions of Northern Ghana. Performance evaluations revealed that the MCA-IoT technology is easy to deploy and manage by users with limited expertise, is location-independent, robust, energy-efficient for battery operation, and scalable in terms of task and size, thereby providing a versatile range of measurements for future applications. Our results further demonstrated that the most effective approach to utilizing existing IoT-based communication technologies within a typical farming context in sub-Saharan Africa is to integrate them. Full article
(This article belongs to the Section Internet of Things)
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19 pages, 779 KiB  
Article
Suppliers’ Perspectives on Cage-Free Eggs in China
by Maria Chen, Huipin Lee, Yuchen Liu and Daniel M. Weary
Animals 2024, 14(11), 1625; https://doi.org/10.3390/ani14111625 - 30 May 2024
Cited by 2 | Viewed by 2777
Abstract
Successful promotion of cage-free eggs supports a housing system offering potential for improved hen welfare. As the world’s largest egg producer and consumer, China offers much potential for welfare improvements. We examined 10 Chinese companies supplying cage-free eggs (four using indoor systems, six [...] Read more.
Successful promotion of cage-free eggs supports a housing system offering potential for improved hen welfare. As the world’s largest egg producer and consumer, China offers much potential for welfare improvements. We examined 10 Chinese companies supplying cage-free eggs (four using indoor systems, six with outdoor access) to understand their strategies to promote cage-free eggs to businesses and consumers. We purposively sampled 12 employees from these companies familiar with production or sales. We conducted two–three semi-structured interviews per participant, collected public online documents (including online shops and social media content), and recorded field notes. We analyzed the data using template analysis to generate key results. Participants reported buyers being unfamiliar with ‘animal welfare’ and ‘cage-free’, but familiar with concepts associated with ‘free-range’. Participants considered three attributes when promoting cage-free eggs: price (engaging buyers who were willing to pay more), experiential attributes (e.g., taste, accommodating buyer preferences), and non-sensory credence attributes (e.g., cage-free production, improving buyers’ understanding and trust). Our results are not generalizable, though they may be transferable to similar contexts. Understanding how companies promoted cage-free eggs to buyers may help inform promotion of other animal products with welfare attributes. Simultaneous efforts are needed to ensure actual welfare improvements on farms. Full article
(This article belongs to the Section Animal System and Management)
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24 pages, 9419 KiB  
Article
Multi-Objective Analysis of Visual, Thermal, and Energy Performance in Coordination with the Outdoor Thermal Environment of Productive Façades of Residential Communities in Guangzhou, China
by Yuyan Wang, Xi Zhang, Yifan Zhang, Hao Zhang, Bo Xiong and Xuepeng Shi
Buildings 2023, 13(6), 1540; https://doi.org/10.3390/buildings13061540 - 16 Jun 2023
Cited by 6 | Viewed by 2556
Abstract
Population growth and urban expansion have led to increased demand for buildings. Optimizing the building façade design, using integrated photovoltaic (PV) shading and vertical farming (VF) can reduce building energy consumption while ensuring a partial food supply. However, the importance and prevalence of [...] Read more.
Population growth and urban expansion have led to increased demand for buildings. Optimizing the building façade design, using integrated photovoltaic (PV) shading and vertical farming (VF) can reduce building energy consumption while ensuring a partial food supply. However, the importance and prevalence of productive façades have not received significant attention. Furthermore, few studies have focused on the impact of productive façades on both indoor and outdoor environmental qualities. Therefore, this study aimed to explore the potential of integrating productive façades with residential façades in high-density cities. A typical community in Guangzhou, China was investigated. Thermal comfort, light comfort, electricity production, and crop yield were considered, and the optimal façade configuration was chosen from the established 146-model library. The integrated module can effectively improve the indoor lighting and thermal comfort of residential buildings. The module also mitigates the outdoor thermal environment to a certain extent, meeting 6.3–10.3% and 7.6–9.6% of the annual electricity and vegetable demands, respectively, in residential communities. This study can guide other densely populated cities with subtropical climates to advance the research and construction of productive façades, improving occupant comfort, reducing energy consumption, and mitigating food security and urban climate change issues. Full article
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12 pages, 1266 KiB  
Article
Fattening Iberian Pigs Indoors vs. Outdoors: Production Performance and Market Value
by Andrés Horrillo, Paula Gaspar, Ángel Muñoz, Miguel Escribano and Elena González
Animals 2023, 13(3), 506; https://doi.org/10.3390/ani13030506 - 31 Jan 2023
Cited by 9 | Viewed by 3197
Abstract
The current Quality Standard regulating the Iberian pig provides for various differentiated farming systems subject to the type of management implemented and the breed of the pigs. This study attempts to analyse the differences between two of these production systems, i.e., the outdoor [...] Read more.
The current Quality Standard regulating the Iberian pig provides for various differentiated farming systems subject to the type of management implemented and the breed of the pigs. This study attempts to analyse the differences between two of these production systems, i.e., the outdoor and the indoor rearing systems by comparing the main technical and economic factors of six farms, three operating under each system, in order to ascertain the most profitable production system. This analysis is based on the information provided by the farm owners. It also evaluates the impact that the COVID-19 pandemic outbreak had on profitability. The results show that both systems spend the same resources on animal feed, which represents nearly 60% of the expense, with the price of purchase of piglets representing 30–32% of the total; however, there are differences in the cost of labour, which is higher in the outdoor variant. In economic terms, outdoor farms obtained a higher gross margin than indoor farms did. Although their production costs are higher, these are offset with larger incomes due to the higher market price of the pigs at the time of slaughter. Lastly, all the farms under study reveal large financial losses on account of COVID-19, given that there was a general decrease in the revenues due to the decrease in the selling price of the pigs, which seems to be the most determinant factor for the economic profits made by these kinds of farms. Full article
(This article belongs to the Special Issue Animals in 2023)
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16 pages, 42118 KiB  
Article
Plant-Wear: A Multi-Sensor Plant Wearable Platform for Growth and Microclimate Monitoring
by Joshua Di Tocco, Daniela Lo Presti, Carlo Massaroni, Stefano Cinti, Sara Cimini, Laura De Gara and Emiliano Schena
Sensors 2023, 23(1), 549; https://doi.org/10.3390/s23010549 - 3 Jan 2023
Cited by 18 | Viewed by 5732
Abstract
Wearable devices are widely spreading in various scenarios for monitoring different parameters related to human and recently plant health. In the context of precision agriculture, wearables have proven to be a valuable alternative to traditional measurement methods for quantitatively monitoring plant development. This [...] Read more.
Wearable devices are widely spreading in various scenarios for monitoring different parameters related to human and recently plant health. In the context of precision agriculture, wearables have proven to be a valuable alternative to traditional measurement methods for quantitatively monitoring plant development. This study proposed a multi-sensor wearable platform for monitoring the growth of plant organs (i.e., stem and fruit) and microclimate (i.e., environmental temperature—T and relative humidity—RH). The platform consists of a custom flexible strain sensor for monitoring growth when mounted on a plant and a commercial sensing unit for monitoring T and RH values of the plant surrounding. A different shape was conferred to the strain sensor according to the plant organs to be engineered. A dumbbell shape was chosen for the stem while a ring shape for the fruit. A metrological characterization was carried out to investigate the strain sensitivity of the proposed flexible sensors and then preliminary tests were performed in both indoor and outdoor scenarios to assess the platform performance. The promising results suggest that the proposed system can be considered one of the first attempts to design wearable and portable systems tailored to the specific plant organ with the potential to be used for future applications in the coming era of digital farms and precision agriculture. Full article
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16 pages, 4586 KiB  
Article
Bacterial Community Characteristics Shaped by Artificial Environmental PM2.5 Control in Intensive Broiler Houses
by Wenxing Wang, Guoqi Dang, Imran Khan, Xiaobin Ye, Lei Liu, Ruqing Zhong, Liang Chen, Teng Ma and Hongfu Zhang
Int. J. Environ. Res. Public Health 2023, 20(1), 723; https://doi.org/10.3390/ijerph20010723 - 30 Dec 2022
Cited by 2 | Viewed by 2049
Abstract
Multilayer cage-houses for broiler rearing have been widely used in intensive Chinese farming in the last decade. This study investigated the characteristics and influencing factors of bacterial communities in the PM2.5 of broiler cage-houses. The PM2.5 samples and environmental variables were collected inside [...] Read more.
Multilayer cage-houses for broiler rearing have been widely used in intensive Chinese farming in the last decade. This study investigated the characteristics and influencing factors of bacterial communities in the PM2.5 of broiler cage-houses. The PM2.5 samples and environmental variables were collected inside and outside of three parallel broiler houses at the early, middle, and late rearing stages; broiler manure was also gathered simultaneously. The bacterial 16S rRNA sequencing results indicated that indoor bacterial communities were different from the outdoor atmosphere and manure. Furthermore, the variations in airborne bacterial composition and structure were highly influenced by the environmental control variables at different growth stages. The db-RDA results showed that temperature and wind speed, which were artificially modified according to managing the needs for broiler growth, were the main factors affecting the diversity of dominant taxa. Indoor airborne and manurial samples shared numerous common genera, which contained high abundances of manure-origin bacteria. Additionally, the airborne bacterial community tended to stabilize in the middle and late stages, but the population of potentially pathogenic bacteria grew gradually. Overall, this study enhances the understanding of airborne bacteria variations and highlighted the potential role of environmental control measures in intensive farming. Full article
(This article belongs to the Section Environmental Microbiology)
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14 pages, 308 KiB  
Article
Prevalence and Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli in Wild Birds from a Wildlife Rescue Centre
by Gaia Casalino, Francesco D’Amico, Francesca Rita Dinardo, Giancarlo Bozzo, Valeria Napoletano, Antonio Camarda, Antonella Bove, Roberto Lombardi, Francesco Paolo D’Onghia and Elena Circella
Animals 2022, 12(20), 2889; https://doi.org/10.3390/ani12202889 - 21 Oct 2022
Cited by 15 | Viewed by 3258
Abstract
Climate change, excessive exploitation of agricultural land which reduces natural habitats, wildlife shooting, and the use of pesticides all cause difficulties for wildlife, with considerable numbers of animals being brought to wildlife rescue centres. Although the efforts of staff involved in wildlife management [...] Read more.
Climate change, excessive exploitation of agricultural land which reduces natural habitats, wildlife shooting, and the use of pesticides all cause difficulties for wildlife, with considerable numbers of animals being brought to wildlife rescue centres. Although the efforts of staff involved in wildlife management at these centres usually focus on therapeutic treatments to reintroduce them into the wild, the monitoring of pathogens that may be transmitted to humans is of relevance. Campylobacter (C.) jejuni and C. coli are frequently carried by animals without inducing clinical signs and are responsible for enteric disorders and more rarely extra-intestinal disease in humans. Farm species and poultry, in particular, are the main reservoirs of C. jejuni and C. coli, but wild animals may also be carriers. The aim of this paper was to investigate the presence of C. jejuni and C. coli in wild birds housed at a wildlife rescue centre and to evaluate the sensitivity of the detected strains to antibiotics. Campylobacter was found in 52 out of 209 (24.88%) birds from 33 different species. C. jejuni was more prevalent, while C. coli was only detected in three Long-eared Owls (Asio otus). The incidence of the infection was particularly high (72.22%) among omnivorous species. Infection rates were higher in birds housed indoors (57.14%) than outdoors (31.74%). Moreover, Campylobacter was not detected in species whose mean temperature body is below 40 °C or higher than 42.2 °C. The most common antibiotic resistance in the tested strains was against trimethoprim/sulfamethoxazole, ciprofloxacin and enrofloxacin. In addition, multi-drug resistance was also found. The results highlight the need to increase biosecurity measures at rescue centres so as to reduce health-related risks to workers involved in wildlife management. Full article
(This article belongs to the Special Issue Zoonoses and Wildlife - One Health Approach)
20 pages, 2304 KiB  
Review
A Flashforward Look into Solutions for Fruit and Vegetable Production
by Léa Maupilé, Adnane Boualem, Jamila Chaïb and Abdelhafid Bendahmane
Genes 2022, 13(10), 1886; https://doi.org/10.3390/genes13101886 - 18 Oct 2022
Cited by 1 | Viewed by 4922
Abstract
One of the most important challenges facing current and future generations is how climate change and continuous population growth adversely affect food security. To address this, the food system needs a complete transformation where more is produced in non-optimal and space-limited areas while [...] Read more.
One of the most important challenges facing current and future generations is how climate change and continuous population growth adversely affect food security. To address this, the food system needs a complete transformation where more is produced in non-optimal and space-limited areas while reducing negative environmental impacts. Fruits and vegetables, essential for human health, are high-value-added crops, which are grown in both greenhouses and open field environments. Here, we review potential practices to reduce the impact of climate variation and ecosystem damages on fruit and vegetable crop yield, as well as highlight current bottlenecks for indoor and outdoor agrosystems. To obtain sustainability, high-tech greenhouses are increasingly important and biotechnological means are becoming instrumental in designing the crops of tomorrow. We discuss key traits that need to be studied to improve agrosystem sustainability and fruit yield. Full article
(This article belongs to the Special Issue Genetic Control of Agronomic Traits in Plants)
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11 pages, 563 KiB  
Article
The Role of Housing Conditions on the Success of Artificial Insemination in Intensively Reared Dairy Ewes in Greece
by Stergios Priskas, Georgios Valergakis, Ioannis Tsakmakidis, Sotiria Vouraki, Vasiliki Papanikolopoulou, Alexandros Theodoridis and Georgios Arsenos
Animals 2022, 12(19), 2693; https://doi.org/10.3390/ani12192693 - 7 Oct 2022
Cited by 3 | Viewed by 2204
Abstract
The objective was to assess the effect of housing conditions during the summer months on the success rates of cervical artificial insemination (AI) with cooled semen, in intensively reared dairy ewes in Greece. The study involved 2083 Lacaune ewes from 23 flocks that [...] Read more.
The objective was to assess the effect of housing conditions during the summer months on the success rates of cervical artificial insemination (AI) with cooled semen, in intensively reared dairy ewes in Greece. The study involved 2083 Lacaune ewes from 23 flocks that were serviced during May to September. An estrous synchronization protocol with the insertion of progestogen sponges for 14 days and eCG administration at sponge removal, was used. All ewes were inseminated 54–57 h after sponge removal with cooled semen (15 °C) from 10 Lacaune rams. Pregnancy diagnosis was performed via trans-dermal ultrasonography at 35–40 days after AI. Data recording started the day after sponge placement (15 days prior to AI), and lasted up to 14 days after AI. Daily records included temperature, relative humidity, and Temperature-Humidity Index (THI) inside the shed. Available space and volume per animal, frequency of bedding renewal, access to a yard, and indoor light were also recorded in each farm. Binary logistic regression of data records showed that temperature and THI increases at days −15 to +4 around AI (day 0) had a negative effect on pregnancy rates (reducing the likelihood of pregnancy by 3–6% and 7%, respectively). The latter also decreased significantly (p < 0.05) in farms with high stocking density, non-frequent bedding renewal, and outdoor access by ewes (by 30%, 34%, and 44%, respectively). Overall, the results indicate that appropriate housing conditions are warranted to increase the success of AI in dairy ewes during the summer months. Full article
(This article belongs to the Special Issue Modern Technology in Farm Animals’ Reproductive Services)
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