Search Results (10,504)

This study evaluated the antimicrobial activity of meads produced by mixed fermentation of Saccharomyces cerevisiae var. boulardii and a kombucha culture, using eucalyptus- and rosemary-monofloral honeys. Antimicrobial activity was investigated in vitro against pathogenic bacteria (Escherichia coli, Salmonella enterica, Listeria innocua, Staphylococcus aureus, and Bacillus cereus) and spoilage yeasts, over 28 days of storage. All tested matrices were autoclaved at 121 °C for 15 min before antimicrobial evaluation to eliminate viable fermentation microorganisms. Additionally, assays were conducted in simulated food systems at different substrate concentrations, characterizing organic acids, reducing sugars, and volatile compounds. Unfermented musts allowed survival of L. innocua and B. cereus, with rosemary honey showing stronger antimicrobial effects than eucalyptus. Kombucha exerted strong initial inhibition, possibly associated with its high organic acid concentrations, but did not completely inhibit the growth of Gram-positive bacteria. No pathogenic bacterial growth was detected in the fermented mead matrices, possibly due to reduced fermentable sugars, ethanol, organic acids, and other fermentation metabolites. Spoilage yeasts showed high tolerance to acid and osmotic stress, and efficacy decreased in substrate-rich systems. Importantly, the antimicrobial activity reported here refers specifically to autoclaved fermented matrices and does not necessarily apply to untreated fermented beverages. Full article

Biorefineries that convert the organic fraction of municipal solid waste (OFMSW) into advanced biofuels can integrate waste management with renewable energy production. However, their environmental performance remains insufficiently characterised owing to a scarcity of life cycle assessment (LCA) studies based on real operational data. This study presents a gate-to-gate LCA of a demonstration biorefinery processing source-separated food waste into bio-oils, bioethanol, and biogas. The ReCiPe 2016 Midpoint (H) method was applied across 18 impact categories, with system expansion crediting the displacement of rapeseed oil, maize-derived ethanol, and marginal biogas-derived electricity. The net global warming potential (GWP) was 68.5 kg CO₂ eq per tonne of wet OFMSW (69% reduction from gross), placing the biorefinery 83–93% below landfilling, 63% below incineration with CHP, and above standalone anaerobic digestion systems that lack the energy-intensive drying and enzymatic hydrolysis steps of the present configuration. Bio-oil and bioethanol achieved net-negative GWP per kilogram of product (−0.89 and −0.66 kg CO₂ eq, respectively), whilst eleven of eighteen categories achieved net savings under system expansion. Enzyme production dominated the bioethanol environmental profile (37–94% across categories), whilst drying dominated bio-oil (46–93%). Monte Carlo simulation confirmed that the sign of the net impact stayed unchanged across the entire 95% confidence interval (the interval did not span zero) for 17 of 18 categories. Full article

Honey is a natural food widely consumed due to its nutritional and functional properties. With the growing consumer interest in organic foods, evaluating the composition and quality parameters of organic and conventional honey products available on the market has become increasingly important. For this purpose, a total of 92 organic and conventional floral honey samples obtained from four different firms in Türkiye were analyzed for total antioxidant status (TAS), total oxidant status (TOS), total phenolic content, proline content, diastase activity, hydroxymethylfurfural (HMF) level, pH, electrical conductivity, color, °Brix, and moisture content. In the floral honey samples, mean values of TAS, TOS, and total phenolic content ranged from 0.48 to 0.93 mmol Trolox equivalent/L, 12.03 to 56.76 µmol H₂O₂ equivalent/L, and 97.17 to 153.00 mg GAE/kg, respectively. Furthermore, the mean values of proline content, diastase number, HMF content, electrical conductivity, and moisture content ranged from 314.27 to 699.81 mg/kg, 8.57 to 28.50, 3.24 to 23.30 mg/kg, 0.20 to 0.28 mS/cm, and 15.70 to 17.39%, respectively, all of which were within the limits specified in the Turkish Food Codex. Additionally, the mean pH and °Brix values ranged from 3.85 to 4.23 and 80.81 to 82.43%, respectively, while the color ranged from white to light amber. Full article

As the global agri-food system faces escalating climate-related challenges, developing localized low-carbon short food supply chains (SFSCs) has become a vital strategy for sustainable agriculture. To elucidate the psychological drivers of green consumer behavior within this context, this study integrates the Stimulus–Organism–Behavior–Consequence (SOBC) framework with the Theory of Planned Behavior (TPB). The aim is to investigate the continuous purchase intentions of Taiwanese consumers for organic cocoa. Data collected via an online survey from 377 consumers with prior cocoa consumption experience were analyzed using Partial Least Squares Structural Equation Modeling (PLS-SEM). The results indicate that personal attitudes and perceived behavioral control positively drive consumption intentions, whereas subjective norms do not. This suggests that consumption behaviors rely on substantive product evaluation rather than social pressures. Additionally, green product attributes and environmental values significantly enhance TPB constructs. Importantly, consumption intentions translate positively into perceived consumer effectiveness and continuous purchase intentions. By elucidating the psychological mechanisms underlying sustainable food choices, this study highlights the value of Taiwan’s “Tree-to-Bar” model. Practically, the findings highlight potential implications for policymakers and agribusinesses regarding how environmental attributes are communicated through front-of-pack (FOP) labels that emphasize local production and low-carbon mileage. Such approaches may help make environmental information more accessible to consumers, although their effectiveness should be evaluated in future research. Full article

Smart chemical sensors have emerged as promising tools for real-time monitoring of food spoilage in both fermented and non-fermented products. By detecting key spoilage indicators—including biogenic amines, ammonia, hydrogen sulfide, methane, pH variations, and microbial volatile organic compounds (MVOCs)—these systems enable rapid, on-site assessment of food quality, offering a viable alternative to conventional, time-consuming laboratory analyses. Recent advances encompass diverse sensing mechanisms, including chemiresistive platforms based on conducting polymers and MEMS (Microelectromechanical Systems); optical/colorimetric systems using dyes, metal–organic frameworks, and porphyrins; and electrochemical and biosensing approaches employing enzymes, antibodies, aptamers, and whole-cell recognition elements. These sensors demonstrate high sensitivity (ppb–ppm range), enabling early detection of spoilage before sensory perception or microbiological threshold exceedance. Their applicability has been validated across a wide range of food matrices, including meat, fish, dairy products, vegetables, beverages, and fermented foods. Despite significant progress, key challenges persist, including signal drift, limited specificity, susceptibility to environmental factors such as humidity and temperature, and interference from complex food matrices. Furthermore, integration into intelligent packaging requires the development of flexible, food-safe, and regulatory-compliant materials. Emerging approaches that combine sensor arrays with machine learning and MVOC pattern recognition are enhancing predictive accuracy and enabling food classification across commodity types. Overall, smart chemical sensing technologies are rapidly transitioning from laboratory prototypes to practical applications in intelligent packaging and wireless monitoring systems, with ongoing research focused on improving robustness, standardization, and scalability for commercial deployment. This article provides an overview of the topic, drawing on the available bibliography from the last five years and the most-cited scientific databases. Full article

The aim of this study was to evaluate the concentrations of 17 heavy metals measured in the liver and kidney tissues of the European brown hare (Lepus europaeus) as a function of age, sex, organ type, and sampling area. In addition, we assessed the relationships between trace element profiles and reproductive parameters and highlighted their potential relevance from a food-safety perspective. Age-related comparisons revealed significantly higher concentrations of Ti, Fe, Cd, and Hg in adult individuals, whereas no statistically significant differences were detected for the remaining elements. No significant sex-related differences were found for any of the investigated elements. In contrast, the organ-based analysis revealed substantial differences. The concentrations of all elements differed significantly between the liver and the kidney. Correlation analyses identified positive associations between Mn and Zn concentrations, as well as between Mn and Cu concentrations, in both organs. The relationship between Zn and Cd was also positive and statistically significant. A comparison among sampling areas showed no significant spatial differences in the concentrations of Cr, Fe, Cu, and Mo. The greatest spatial heterogeneity was observed for Co, Hg, Pb, and Se, with Se concentrations differing significantly among nearly all sampling areas. Our results indicate strongly overlapping spatial heavy metal profiles, suggesting a gradual rather than sharply differentiated geographical pattern and similar emission characteristics. This interpretation is further supported by the clustering and principal component analysis (PCA) results. PERMANOVA analyses indicated that trace element profiles did not significantly influence the number of placental scars. Furthermore, neither age nor its interaction with trace element profiles exerted a detectable effect on variation in placental scar numbers. Full article

Background/Objectives: Choline is an essential nutrient, and deficiency is associated with metabolic dysfunction-associated steatotic liver disease. Research shows that Western populations have inadequate choline intake; however, the intake in Asian populations is less studied. This study developed a choline database for Thai food and evaluated intake in the Thai population. Methods: Following guidelines of the Food and Agriculture Organization/the International Network of Food Data Systems, 1806 Thai food items were matched to the U.S. choline database, and the missForest imputation method was used to impute missing data. Choline intake was assessed in Thai adults aged 18 years and older using a nationally representative sample of the Thai Food Consumption Survey (n = 3593). Results: Animal-based foods such as eggs, meat, and fish contained more choline than plant-based foods. Median choline intake was 134.7 mg/day (IQR: 70.6, 229.3), with 3.6% of Thai individuals meeting adequate intake levels. Men consumed more choline than women (median 153 vs. 120 mg/day; p < 0.001). Choline intake declined with age but did not differ by body mass index. Conclusions: This study created a choline database for Thai food and shows that most Thais have intake below the recommended intake levels. Full article

Small-scale fisheries are socio-ecological systems in which resource users interact with environmental conditions, fishing regulations, community initiatives, and institutional actors. Understanding how fishers perceive and report these interactions provides information on the social context of fisheries management, although such perceptions cannot substitute for independent assessments of ecological sustainability, behavioural compliance, or governance effectiveness. This study characterized fishers’ environmental perceptions, self-reported fishing practices, participation in environmental education and conservation activities, and perceptions of community and institutional involvement in fisheries management in Tecomate Lagoon, Guerrero, Mexico. A cross-sectional quantitative design was used, and structured questionnaires were administered to 120 active artisanal fishers selected through simple random sampling. Data were analyzed descriptively using frequencies and percentages. Respondents primarily associated the lagoon with economic (49.17%) and food-related (25.83%) functions. Declining water levels (60.00%) and reduced species abundance (40.00%) were the environmental changes reported by respondents. Regarding fishing practices, 94.17% stated that they complied with fishing regulations, and 100% reported releasing organisms below the minimum legal catch size. Environmental training was reported by 95.00% of respondents, while 94.17% reported participation in conservation-related activities. CONAPESCA (80.83%) and UAGro (19.17%) were identified as institutions involved in fisheries-related initiatives. These findings characterize the perceptions and self-reported experiences of surveyed fishers and should not be interpreted as evidence of ecological sustainability, actual behavioural compliance, educational effectiveness, or governance performance. The contribution of the study lies in systematically documenting the resource-user perspective as a component of the social dimension of the fishery. This information provides a descriptive baseline that can complement future ecological, behavioural, institutional, and longitudinal assessments of small-scale fisheries in coastal lagoon systems. Full article

The growing demand for eggs from alternative housing systems is driven by perceived nutritional and welfare benefits. However, the trade-off between enhanced bioactive composition and exposure to environmental contaminants remains insufficiently explored. This study evaluated the physical traits, bioactive compounds, and heavy metal content of 480 commercially available table eggs from four housing systems: organic, free-range, barn, and enriched cages. Organic eggs exhibited the highest albumen quality, expressed by Haugh units (92.03 vs. 70.86 in cages) and lysozyme activity (144,877 U/mL vs. 119,830 U/mL in cages), alongside the highest concentrations of fat-soluble vitamins (e.g., vitamin A at 1600 μg/100 g and vitamin E at 3267 μg/100 g) and n-3 polyunsaturated fatty acids, including docosahexaenoic acid (DHA: 0.673% vs. 0.360% in cages). Conversely, organic eggs presented the weakest eggshells (breaking strength of 34.19 N vs. 47.05 N in cages) and a less favourable lipid profile regarding atherogenicity (AI: 0.411) and thrombogenicity (TI: 3.455) indices. Crucially, organic eggs contained significantly higher levels of lead (0.361 mg/kg) compared to indoor systems (0.041 mg/kg in barn and 0.102 mg/kg in cages), highlighting their susceptibility to environmental pollution. Cage and barn eggs were associated with higher chemical safety and shell strength but demonstrated a lower overall bioactive value. Ultimately, the choice of table eggs may involve a trade-off between the health-promoting benefits of extensive systems and the potential risks of heavy metal contamination, emphasising the need for integrated food safety management. Full article

An evaluation of the safety profile of palatinose/fructooligosaccharide (P/FOS) and palatinose/invert sugar (P/IS), as novel low-glycemic and functional sweetener combinations, is essential for their application. This study aimed to evaluate the safety of 1000 mg/kg/day of P/FOS and 1000 mg/kg/day of P/IS through a 14-day repeated-dose oral toxicity study using male and female Sprague–Dawley (SD) rats. Clinical signs, such as body weight and organ weights, food/water consumption, hematology results, blood biochemical analysis results, urinalysis results, and histological changes in the liver and kidneys, were recorded in all SD rats. Barely any significant changes in food/water consumption, body weight, and organ weight were observed during the experimental period. Although there were some alterations in the results of hematological analysis, serum biochemical analysis, and urinalysis, these changes were not considered toxicologically significant within the normal ranges in both male and female SD rats administered with P/FOS and P/IS at 1000 mg/kg/day. Additionally, a histopathological analysis of the liver and kidneys showed no toxicological changes in P/FOS- and P/IS-treated SD rats of both sexes at the tested dose. Collectively, the above results confirmed that no overt treatment-related adverse effects of P/FOS and P/IS were observed under the tested conditions. Full article

Certification schemes are increasingly positioned as institutional mechanisms that may support sustainable food markets, yet the joint longitudinal relationship between certified production penetration, certified supply-chain infrastructure and organic market uptake remains insufficiently integrated in European-scale empirical analyses. This study examined how certified organic production, supply-chain infrastructure, and market uptake co-evolved across Europe. A country–year panel of 995 observations from 41 European countries (2000–2024) was assembled from FiBL statistics and analysed using descriptive trend analysis, Spearman correlations, linear mixed-effects models with country random intercepts, and k-means clustering. Certified organic systems expanded substantially: mean organic farmland share rose from 2.80% to 9.73%, retail sales share from 1.42% to 4.49%, and per capita consumption from €25.82 to €87.60. Organic area share remained positively associated with organic retail sales share after adjustment for PPP-adjusted GDP per capita (β = 1.247, p < 0.001), with a similar pattern for the secondary per capita consumption outcome (β = 36.426, p < 0.001). Certified supply-chain actors were strongly intercorrelated (producers–processors ρ = 0.824). Organic farmland share was the most consistent correlate of market uptake, including after adjustment for GDP per capita, whereas absolute operator counts and supply-chain infrastructure indicators were more difficult to interpret independently because of collinearity. A four-cluster typology showed that countries with the largest certified production bases were not those with the most mature markets. These findings are consistent with the interpretation that certification penetration may support market development by providing credible institutional signals, but trust creation, information asymmetry reduction, and legitimacy were not directly measured. The results, therefore, indicate an association between certification-system development and market uptake, while also showing that production expansion and market maturity are distinct dimensions of the sustainability transition. Full article

Selecting suitable agricultural land is critical for food security and sustainable development, particularly in arid regions facing resource scarcity and environmental degradation. This study assesses agricultural land suitability in Sohag governorate, Egypt, using a hybrid Geographic Information System (GIS), Fuzzy Analytical Hierarchy Process (FAHP) and geostatistical approach. Thirty-four representative soil profiles were morphologically described and analyzed for ten physical (slope, depth, erosion, texture, stoniness, drainage) and chemical (EC, pH, CaCO₃, organic matter) criteria, weighted using both the Analytical Hierarchy Process (AHP) and FAHP. Geostatistical analysis characterized the spatial variability in soil properties across the study area. The two suitability maps were validated using Receiver Operating Characteristic (ROC) curves and Kappa statistics: the FAHP achieved higher prediction accuracy (AUC = 0.83, Kappa = 0.91) than the AHP (AUC = 0.81, Kappa = 0.88). The AHP-based map classified 40% (1154.92 km²) as moderately suitable (S2), 33% (952.81 km²) as marginally suitable (S3), and 27% (779.57 km²) as unsuitable (N); the FAHP-based map classified 42% (1212.67 km²) as S2, 30% (866.19 km²) as S3, and 28% (808.44 km²) as N. The proposed framework may serve as a reference for similar arid environments and support progress toward Sustainable Development Goals 2, 6, 8, 9, 11, 13 and 15, conditional on local soil, water, climate and management conditions. Full article

Freshwater crayfish impact ecosystems and the food industry. The aim of the study was to valorize shells from the invasive crayfish, Faxonius limosus, to extract novel bioactive biopolymers and prepare biodegradable films, thereby contributing to economic and environmental sustainability. Chitin from crayfish shells was isolated and converted into chitosan through a new protocol to retain astaxanthin, with a shorter procedure, reduced use of organic solvent and energy consumption. The resulting biopolymers were used to prepare chitosan-based films. Thermal, spectroscopic, barrier properties, and surface microstructure of the biomaterials were identified. Spectrophotometric and chromatographic techniques were used on crayfish shells and biopolymers to track astaxanthin throughout the extraction and formulation processes. Crayfish chitosan film exhibited higher stiffness, enhanced thermal stability and water vapor barrier properties with respect to commercial chitosan films. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl antioxidant assays showed that all crayfish biomaterials exhibited antioxidant activity. Astaxanthin greatly enhanced the antioxidant properties of crayfish chitosan, but chemical degradation during film processing limited this effect in the film matrices. Therefore, crayfish-derived biopolymers and films are promising for incorporation into functional foods and active packaging and support shell waste reduction, offering a viable strategy for managing and valorizing invasive species. Full article

Listeria monocytogenes (LM), one of the most virulent foodborne pathogens, poses a serious threat to public health due to its strong environmental adaptability and high pathogenicity. Rapid, sensitive, and real-time detection of LM is of great importance. Chemiresistive gas sensors have attracted enormous attention in LM detection owing to their advantages of low cost, simple structure, fast response, and easy miniaturization, which can achieve indirect detection of LM by recognizing its specific metabolic volatile organic compounds. This review summarizes the recent progress in chemiresistive gas sensors for the detection of LM metabolites. First, the metabolic characteristics of LM and the typical volatile organic compound (3-hydroxy-2-butanone) as its characteristic biomarker are introduced. Then, the performance and sensing mechanisms of different types of chemiresistive gas sensors for LM metabolite detection are summarized and elaborated systematically. The application of chemiresistive gas sensors for the detection of actual samples and the progress in the design of related detection devices are introduced. Finally, the current challenges faced by chemiresistive gas sensors in LM metabolite detection and their future development prospects are discussed. This review provides a comprehensive reference for the research and practical application of chemiresistive gas sensors in Listeria monocytogenes detection. Full article

Urban air pollution remains a major public health concern, with road traffic representing one of the dominant sources of particulate matter and volatile organic compounds in growing cities. This study evaluates the level of chemical air pollution in Alba-Iulia municipality (Romania) through two measurement campaigns (May 2025 and October 2025), carried out at 26 and 19 points, respectively, selected based on traffic intensity and population vulnerability criteria (schools, kindergartens, hospitals, and the food market). Measurements targeted PM_2.5, PM₁₀, total volatile organic compounds (TVOC) and formaldehyde (HCHO), as well as carbon dioxide (CO₂), correlated with road traffic intensity. Statistical analysis revealed significant differences between the two seasons for PM_2.5, PM₁₀, and CO₂ (p < 0.01), as well as a strong correlation between particulate matter concentrations and vehicle counts (r = 0.60–0.95), consistent with road traffic being an important local contributor to particulate matter, though correlation alone cannot establish source dominance in a strict causal sense. A multiple regression controlling for both traffic and season explained over 73% of the variance in PM_2.5 and PM₁₀ and showed that traffic and season each contribute independently to particulate levels. Both mean PM_2.5 and PM₁₀ exceeded WHO and EU limit values in both campaigns, and the low-cost sensor over-read absolute concentrations by roughly 2–4× relative to the official monitoring network, so absolute values should be treated as orientative. Despite this bias, the consistent spatial and seasonal patterns show that portable low-cost sensors can reliably rank exposure hotspots and support the targeting of traffic-mitigation measures, such as selective catalytic reduction (SCR) systems, in medium-sized cities that lack dense reference monitoring networks. Full article