Search Results (1,731)

In the rock–soil–biology–water ecosystem, rock weathering provides essential plant nutrients. However, its supply is insufficient for rising crop demands under population growth and climate change, while excessive fertilizer causes soil degradation and pollution. This study innovatively irrigated with carbonate rock leachates to enhance soil nutrient availability. A pot experiment with lettuce showed that irrigation significantly increased soil NO₃⁻-N (+102.20%), available K (+16.45%), available P (+17.95%), Ca (+6.04%), Mg (+11.65%), and Fe (+11.60%), and elevated the relative abundance of Firmicutes. Lettuce biomass per plant rose by 23.78%, with higher leaf minerals (P, K, Ca, and Mg) and antioxidants (carotenoids and ascorbic acid). A field experiment further confirmed improvement of soil nutrient availability and peanut yield. This carbonate rock leachate irrigation technique effectively enhances soil quality and crop productivity/quality, offering a sustainable approach for green agriculture. Full article

Background: BDE-47, a pervasive environmental pollutant detected in >90% of human serum samples, is increasingly linked to metabolic disorders. This study investigates the specific impact of BDE-47 exposure on the gut microbiota in prediabetic mice and evaluates the efficacy of therapeutic interventions in mitigating these effects. Objectives: To determine whether BDE-47 exposure induces diabetogenic dysbiosis in prediabetic mice and to assess whether dietary interventions, such as grape exosomes and an antioxidant cocktail, can restore a healthy microbiota composition and mitigate diabetes risk. Methods: In this study, a prediabetic mouse model was established in 54 male SPF-grade C57BL/6J mice through a combination of high-sugar and high-fat diet feeding with streptozotocin injection. Oral glucose tolerance tests (OGTT) were conducted on day 7 and day 21 post-modeling to assess the establishment of the model. The criteria for successful model induction were defined as fasting blood glucose levels below 7.8 mmol/L and 2 h postprandial glucose levels between 7.8 and 11.1 mmol/L. Following confirmation of model success, a 3 × 3 factorial design was applied to allocate the experimental animals into groups based on two independent factors: BDE-47 exposure and exosome intervention. The BDE-47 exposure factor consisted of three dose levels—none, high-dose, and medium-dose—while the exosome intervention factor included three modalities—none, Antioxidant Nutrients Intervention, and Grape Exosomes Intervention. Fresh fecal samples were collected from mice two days prior to sacrifice. Cecal contents and segments of the small intestine were collected and transferred into 1.5 mL cryotubes. All sequences were clustered into operational taxonomic units (OTUs) based on defined similarity thresholds. To compare means across multiple groups, a two-way analysis of variance (ANOVA) was employed. The significance level was predefined at α = 0.05, and p-values < 0.05 were considered statistically significant. Bar charts and line graphs were generated using GraphPad Prism version 9.0 software, while statistical analyses were performed using SPSS version 20.0 software. Results: The results of 16S rDNA sequencing analysis of the microbiome showed that there was no difference in the α diversity of the intestinal microbiota in each group of mice (p > 0.05), but there was a difference in the Beta diversity (p < 0.05). At the gate level, the abundances of Proteobacteria, Campylobacterota, Desulfobacterota, and Fusobacteriota in the medium-dose BDE-7 group were higher than those in the model control group (p < 0.05). The abundance of Patellar bacteria was lower than that of the model control group (p < 0.05). The abundances of Proteobacteria and Campylobacterota in the high-dose BDE-7 group were higher than those in the model control group (p < 0.05). The abundance of Planctomycetota and Patescibacteria was lower than that of the model control group (p < 0.05), while the abundance of Campylobacterota in the grape exosome group was higher than that of the model control group (p < 0.05). The abundance of Patescibacteria was lower than that of the model control group (p < 0.05), while the abundance of Firmicutes and Fusobacteriota in the antioxidant nutrient group was higher than that of the model control group (p < 0.05). However, the abundance of Verrucomicrobiota and Patescibacteria was lower than that of the model control group (p < 0.05). At the genus level, the abundances of Bacteroides and unclassified Lachnospiraceae in the high-dose BDE-7 group were higher than those in the model control group (p < 0.05). The abundance of Lachnospiraceae NK4A136_group and Lactobacillus was lower than that of the model control group (p < 0.05). The abundance of Veillonella and Helicobacter in the medium-dose BDE-7 group was higher than that in the model control group (p < 0.05), while the abundance of Lactobacillus was lower (p < 0.05). The abundance of genera such as Lentilactobacillus and Faecalibacterium in the grape exosome group was higher than that in the model control group (p < 0.05). The abundance of Alloprevotella and Bacteroides was lower than that of the model control group (p < 0.05). In the antioxidant nutrient group, the abundance of Lachnospiraceae and Hydrogenophaga was higher than that in the model control group (p < 0.05). However, the abundance of Akkermansia and Coriobacteriaceae UCG-002 was significantly lower than that of the model control group (p < 0.05). Conclusions: BDE-47 induces diabetogenic dysbiosis in prediabetic mice, which is reversible by dietary interventions. These findings suggest that microbiota-targeted strategies may effectively mitigate the diabetes risk associated with environmental pollutant exposure. Future studies should further explore the mechanisms underlying these microbiota changes and the long-term health benefits of such interventions. Full article

The growing environmental concerns associated with petroleum-based plastics require the development of sustainable, biodegradable alternatives. Polyhydroxyalkanoates (PHAs), a family of biodegradable bioplastics, offer a promising potential as eco-friendly substitutes due to their renewable origin and favorable degradation properties. This research investigates the use of synthetic condensate, mimicking the liquid fraction from drying and shredding of household food waste, as a viable substrate for PHA production using mixed microbial cultures. Two draw-fill reactors (DFRs) were operated under different feed organic concentrations (2.0 ± 0.5 and 3.8 ± 0.6 g COD/L), maintaining a consistent carbon-to-nitrogen ratio to selectively enrich microorganisms capable of accumulating PHAs through alternating nutrient availability and deficiency. Both reactors achieved efficient organic pollutant removal (>95% soluble COD removal), stable biomass growth, and optimal pH levels. Notably, the reactor with the higher organic load (DFR-2) demonstrated a modest increase in PHA accumulation (19.05 ± 7.18%) compared to the lower-loaded reactor (DFR-1; 15.19 ± 6.00%), alongside significantly enhanced biomass productivity. Polymer characterization revealed the formation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), influenced by the substrate composition. Microbial community analysis showed an adaptive shift towards Proteobacteria dominance, signifying successful enrichment of effective PHA producers. Full article

Plastic pollution has recently become a serious environmental problem, since the continuous increase in plastic production and use has generated enormous amounts of plastic waste that decomposes to form micro- and nanoparticles (MPs/NPs). Recent evidence suggests that nanoplastics may be potent toxins because they are able to freely cross biological barriers, posing health risks, particularly to developing organisms. Therefore, the aim of the current study was to investigate the toxic potential of polystyrene nanoparticles (PS-NPs) on the jejunum of immature rats. Two-week-old animals were orally exposed to environmentally relevant dose of small PS-NPs (1 mg/kg b.w.; 25 nm) for 3 weeks. We detected a significant accumulation of PS-NPs in the epithelium and subepithelial layer of the intestine, which resulted in significant changes in the expression of genes related to gut barrier integrity, nutrient absorption, and endocrine function. Moreover, increased expression of proinflammatory cytokines was observed together with decreased antioxidant capacity and increased markers of oxidative damage to proteins. Additionally, in the jejunal extracts of exposed rats, we also noted changes in the metabolite profile, mainly amino acids involved in molecular pathways related to cellular energy, inflammation, the intestinal barrier, and protein synthesis, which were consistent with the observed molecular markers of inflammation and oxidative stress. Taken together, the results of the metabolomic, molecular, and biochemical analyses indicate that prolonged exposure to PS-NPs may disrupt the proper function of the intestine of developing organisms. Full article

This study investigates growth responses, heavy metal (Cd, As) uptake, translocation, and mineral nutrient regulation in leafy vegetables with varying heavy metal tolerance, addressing the threat posed by combined Cd and As pollution. Three high-tolerance, four moderate-tolerance, and one sensitive leafy vegetable were grown in Cd+As-contaminated hydroponics. Post-harvest yields and concentrations of Cd, As, and trace elements were assessed. Results showed that (1) compared with single heavy metal treatments, the combination of Cd and As significantly increased the translocation factor of Cd in black bean sprouts and white radish sprouts by up to 83.83% and 503.2%; (2) changes in mineral nutrient concentrations in leafy vegetables were similar between single and combined heavy metal stresses, but the regulatory patterns varied among different leafy vegetable species; (3) under Cd/As exposure, high-tolerance leafy vegetables (e.g., pak choi) had strong heavy metal accumulation abilities, and heavy metal stress positively regulated mineral elements in their roots; In contrast, sensitive leafy vegetables (e.g., pea sprouts) often exhibited suppressed mineral element content in their roots, which was a result of their strategy to reduce heavy metal uptake. These results offer key insights into resistance mechanisms against combined heavy metal pollution in leafy vegetables, supporting phytoremediation efforts and safe production. Full article

Air pollution acts as a pervasive oxidative stressor, disrupting global crop production and ecosystem health through the overproduction of reactive oxygen species (ROS). Hazardous pollutants impair critical physiological processes—photosynthesis, respiration, and nutrient uptake—triggering oxidative damage and yield losses. This review synthesizes current knowledge on plant defense mechanisms, emphasizing the integration of enzymatic (SOD, POD, CAT, APX, GPX, GR) and non-enzymatic (polyphenols, glutathione, ascorbate, phytochelatins) antioxidant systems to scavenge ROS and maintain redox homeostasis. We highlight the pivotal roles of transcription factors (MYB, WRKY, NAC) in orchestrating stress-responsive gene networks, alongside MAPK and phytohormone signaling (salicylic acid, jasmonic acid, ethylene), in mitigating oxidative stress. Secondary metabolites (flavonoids, lignin, terpenoids) are examined as biochemical shields against ROS and pollutant toxicity, with evidence from transcriptomic and metabolomic studies revealing their biosynthetic regulation. Furthermore, we explore biotechnological strategies to enhance antioxidant capacity, including overexpression of ROS-scavenging genes (e.g., TaCAT3) and engineering of phenolic pathways. By addressing gaps in understanding combined stress responses, this review provides a roadmap for developing resilient crops through antioxidant-focused interventions, ensuring sustainability in polluted environments. Full article

Reducing the application of chemical fertilizers and remediating heavy metal pollution in soil are important directions in current agricultural research. Utilizing the plant-growth-promoting and remediation capabilities of bacteria can provide more environmentally friendly assistance to agricultural production. In this study, the Burkholderia alba YIM B08401 strain was isolated and identified from rhizospheric soil, subjected to whole-genome sequencing and analysis, and its Cd²⁺ adsorption efficiency and characteristics were confirmed using multiple experimental methods, including atomic absorption spectrometry (AAS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS). The results showed that the genome of strain YIM B08401 has a total length of 7,322,157 bp, a GC content of 66.39%, and predicts 6504 protein-coding sequences. It contains abundant functional genes related to nutrient conversion (phosphate solubilization, sulfur metabolism, zinc solubilization, siderophore production), plant hormone regulation (indole-3-acetic acid secretion, ACC deaminase production), phenolic acid degradation, root colonization, heavy metal tolerance, pathogen antagonism, and the production of antagonistic secondary metabolites. Additionally, strain YIM B08401 can specifically bind to Cd²⁺ through various functional groups on the cell surface, such as C-O-C, P=O, and O-H, enabling biosorption. In conclusion, strain YIM B08401 is an excellent strain with plant-growth-promoting, disease-resistant, and bioremediation capabilities, warranting further development as a biofertilizer for agricultural applications to promote green and sustainable agricultural development. Full article

The practice in agriculture of spreading polyethylene (PE) film over the soil surface as mulch is a common, global practice that aids in conserving water, increasing crop yields, suppressing weed growth, and decreasing growing time. However, these films are typically only used for a single growing season, and thus, their use and non-biodegradability come with some serious environmental consequences due to their persistence in the soil and potential for microplastic pollution, particularly when retrieval and disposal options are poor. On the microscale, particles < 5 mm from degraded films have been observed to disrupt soil structure, impede water and nutrient cycling, and affect soil organisms and plant health. On the macroscale, there are obvious and serious environmental consequences associated with the burning of plastic film and its leakage from poorly managed landfills. To maintain the crop productivity afforded by mulching with PE film while avoiding the environmental downsides, the development and use of biodegradable polymer technologies is being explored. Here, the efficacy of a newly developed, water-dispersible, sprayable, and biodegradable polyester–urethane–urea (PEUU)-based polymer was compared with two commercial PE mulches, non-degradable polyethylene (NPE) and OPE (ox-degradable polyethylene), in a greenhouse tomato growth trial. Water savings and the effects on plant growth and soil characteristics were studied. It was found that PEUU provided similar water savings to the commercial PE-based mulches, up to 30–35%, while showing no deleterious effects on plant growth. The results should be taken as preliminary indications that the sprayable, biodegradable PEUU shows promise as a replacement for PE mulch, with further studies under outside field conditions warranted to assess its cost effectiveness in improving crop yields and, importantly, its longer-term impacts on soil and terrestrial fauna. Full article

This study aimed to analyse the spatiotemporal evolution of phytoplankton community dynamics and its underlying mechanisms in the Liaoning section of the Liao River Basin in 2010, 2015, and 2020. Phytoplankton species diversity increased significantly, with an increase from three phyla and 31 species in 2010 to six phyla and 74 species in 2020. Concurrent increases in α-diversity indicated continuous improvements in habitat heterogeneity. The community structure shifted from a diatom-dominated assemblage to a green algae–diatom co-dominated configuration, contributing to an enhanced water purification capacity. The upstream agricultural zone (Tieling section) had elevated biomass and low diversity, indicating persistent non-point-source pollution stress. The midstream urban–industrial zone (Shenyang–Anshan section) emerged as a phytoplankton diversity hotspot, likely due to expanding niche availability in response to point-source pollution control. The downstream wetland zone (Panjin section) exhibited significant biomass decline and delayed diversity recovery, shaped by the dual pressures of resource competition and habitat filtering. The driving mechanism of community succession shifted from nutrient-dominated factors (NH₃-N, TN) to redox-sensitive factors (DO, pH). These findings support a ‘zoned–graded–staged’ ecological restoration strategy for the Liao River Basin and inform the use of phytoplankton as bioindicators in watershed monitoring networks. Full article

Meloidogyne enterolobii, a highly virulent and broad-host-range plant-parasitic nematode, poses an increasing threat to global agricultural production. By inducing the formation of nutrient-rich giant cells in host roots and deploying a diverse array of effector proteins to modulate plant immune responses, this nematode achieves efficient colonization and invasion, resulting in impaired crop growth and significant economic losses. In recent years, global climate warming combined with the rapid development of protected agriculture has broken the traditional geographical limits of tropical and subtropical regions, thereby increasing the risk of M. enterolobii occurrence in temperate and high-latitude areas. Concurrently, conventional chemical control methods are increasingly limited by environmental pollution and the development of resistance, steering research toward green control strategies. This review systematically summarizes the latest research progress of M. enterolobii in terms of ecological diffusion trends, pathogenic mechanisms, and green control, and explored the feasibility of integrating multidisciplinary technologies to construct an efficient and precise control system. The ultimate aim is to provide theoretical support and technical supports for green and sustainable development of global agriculture. Full article

Soil acidification, salinization, and heavy metal pollution pose serious threats to global food security and sustainable agricultural development. Biochar, with its high porosity, large surface area, and abundant functional groups, can effectively improve soil properties. However, due to variations in feedstocks and pyrolysis conditions, it may contain potentially harmful substances. Industrial wastes such as fly ash, steel slag, red mud, and phosphogypsum are rich in minerals and show potential for soil improvement, but direct application may pose environmental risks. The co-application of biochar with these wastes can produce composite amendments that enhance pH buffering capacity, nutrient availability, and pollutant immobilization. Therefore, a review of biochar-industrial waste composites as soil amendments is crucial for addressing soil degradation and promoting resource utilization of wastes. In this study, the literature was retrieved from Web of Science, Scopus, and Google Scholar using keywords including biochar, fly ash, steel slag, red mud, phosphogypsum, combined application, and soil amendment. A total of 144 articles from 2000 to 2025 were analyzed. This review summarizes the physicochemical properties of biochar and representative industrial wastes, including pH, electrical conductivity, surface area, and elemental composition. It examines their synergistic mechanisms in reducing heavy metal release through adsorption, complexation, and ion exchange. Furthermore, it evaluates the effects of these composites on soil health and crop productivity, showing improvements in soil structure, nutrient balance, enzyme activity, and metal immobilization. Finally, it identifies knowledge gaps as well as future prospects and recommends long-term field trials and digital agriculture technologies to support the sustainable application of these composites in soil management. Full article

The demand for rapid, portable, and cost-effective analytical tools has driven advances in smartphone-based microfluidic sensors. By combining microfluidic precision with the accessibility and processing power of smartphones, these devices offer real-time and on-site diagnostic capabilities. This review explores recent developments in smartphone-integrated microfluidic sensors, focusing on their design, fabrication, smartphone integration, and analytical functions with the applications in forensic science, agriculture, and environmental monitoring. In forensic science, these sensors provide fast, field-based alternatives to traditional lab methods for detecting substances like DNA, drugs, and explosives, improving investigation efficiency. In agriculture, they support precision farming by enabling on-demand analysis of soil nutrients, water quality, and plant health, enhancing crop management. In environmental monitoring, these sensors allow the timely detection of pollutants in air, water, and soil, enabling quicker responses to hazards. Their portability and user-friendliness make them particularly valuable in resource-limited settings. Overall, this review highlights the transformative potential of smartphone-based microfluidic sensors in enabling accessible, real-time diagnostics across multiple disciplines. Full article

Algal and cyanobacterial blooms are anticipated to increase in frequency, duration, and geographic extent as a result of environmental changes, including climate warming, elevated nutrient concentrations, and increased runoff in both marine and freshwater ecosystems. The eutrophication of aquatic environments represents a substantial threat to human health. As eutrophication progresses, airborne algae and cyanobacteria, particularly harmful genera originating from aquatic environments, are released into the atmosphere and may pose potential risks to human health. Furthermore, respiratory distress has been documented in individuals exposed to aerosols containing harmful algal bloom (HAB) toxins. This review investigates the generation of aerosolised harmful algal blooms, their responses to environmental factors, and their associated health risks. Evidence suggests that airborne algae, cyanobacteria, and their toxins are widespread. When these are aerosolised into micrometre-sized particles, they become susceptible to atmospheric processing, which may degrade the HAB toxins and produce byproducts with differing potencies compared to the parent compounds. Inhalation of aerosolised HAB toxins, especially when combined with co-morbid factors such as exposure to air pollutants, could present a significant health risk to a considerable proportion of the global population. A more comprehensive understanding of the chemical transformations of these toxins and the composition of harmful algal and cyanobacterial communities can improve public safety. Full article

Straw returning inhibits tillering at the early stage of rice growth and thus affects grain yield. Sulfur-coated urea (SCU) has been expected to increase nitrogen use efficiency (NUE) and yield, save labor input, and reduce environmental pollution in crop production. Nevertheless, the sulfur coatings of SCU are easy to break and then shorten the nutrient release cycle. Whether there was a complementary effect between straw returning and SCU in NUE and grain yield had remained elusive. To investigate the effects of straw returning combined with the application of SCU on NUE and rice yield, a two-year field experiment was conducted from 2022 to 2023 with three treatments (straw returning combined with conventional urea (SRU), no straw returning combined with SCU (NRS), straw returning combined with SCU (SRS)). We found that straw returning combined with the application of SCU increased rice yield and NUE significantly. Compared with SRU and NRS, SRS treatments significantly increased grain yield by 14.61–16.22%, and 4.14–7.35%, respectively. Higher effective panicle numbers per m² and grain numbers per panicle were recorded in NRS and SRS treatments than SRU. SRS treatment increased nitrogen recovery efficiency by 79.53% and 22.97%, nitrogen agronomic efficiency by 18.68% and 17.37%, and nitrogen partial factor productivity by 10.51% and 9.81% compared with SRU and NRS treatment, respectively. The enhanced NUE in SRS was driven by higher leaf area index, SPAD value, net photosynthetic rate, carbon metabolic enzyme (RuBP and SPS) activity, nitrogen metabolic enzyme (NR, GS, and GOGAT) activity, sucrose and nitrogen content in leaves, and nitrogen accumulation in plant during grain filling. Moreover, the improved yield in SRS was closely related to superior NUE. In conclusion, straw returning combined with application of SCU boosted grain yield and NUE via enhanced carbon–nitrogen metabolism during the late growth period in rice. Full article

Accurate quantification of soil organic matter (SOM) is crucial for improving soil fertility and maintaining ecosystem health. The content of SOM affects soil nutrient availability and is closely linked to the global carbon cycle. The use of an electronic nose to detect SOM contents has the advantages of rapidity, accuracy, and low pollution to the environment. This study proposes a method for obtaining SOM contents via pyrolysis coupled with an artificial olfaction system. To improve the accuracy of SOM content determination, the effects of three parameters (pyrolysis temperature, pyrolysis time, and soil sample mass) related to the pyrolysis process on the distinguishability of pyrolysis gases were investigated. Firstly, single-factor experiments were conducted to determine the optimal values of three parameters that can improve the differentiation of pyrolysis gases. Secondly, a regression model based on the Box–Behnken experiment was established to analyze the interrelationships between the three parameters and the discrete ratio. The experimental results showed that the three parameters exerted significant influences on the discrete ratio, with pyrolysis time having the greatest impact, followed by soil sample mass and pyrolysis temperature. The optimal discrimination and minimal dispersion ratio of the pyrolysis gases were achieved at a pyrolysis temperature of 384 °C, with a pyrolysis time of 2 min 41 s and a soil sample mass of 1.68 g. Finally, the Back-Propagation Neural Network (BPNN) and Partial Least-Squares Regression (PLSR) algorithms were used to establish an SOM prediction model after obtaining soil pyrolysis gases under the optimal combination of pyrolysis parameters. The experimental results demonstrated that the SOM prediction model based on PLSR achieved the best accuracy and the highest generalization capability, with R² > 0.85 and RMSE < 7.21. This study could provide a theoretical basis for the prediction of SOM contents via pyrolysis coupled with an artificial olfaction system. Full article