Search Results (29)

Silk fibroin (SF)-based intelligent wearable systems represent a frontier research direction in artificial intelligence and precision medicine. Their core efficacy stems from the inherent advantages of silk fibroin, including excellent mechanical properties, interfacial compatibility, and tunable structure. This article systematically reviews conductive modification strategies for silk fibroin and its research progress in the smart wearable field. It elaborates on the molecular structural basis of silk fibroin for use in smart wearable devices, critically analyzes five conductive functionalization strategies, compares the advantages, disadvantages, and applicable domains of different modification approaches, and summarizes research achievements in areas such as bioelectrical signal sensing, energy conversion and harvesting, and flexible energy storage. Concurrently, an assessment was conducted focusing on the priority performance characteristics of the materials across diverse application scenarios. Specific emphasis was placed on addressing the long-term functional performance (temporal efficacy) and degradation stability of silk fibroin-based conductive materials exhibiting high biocompatibility in implantable settings. Additionally, the compatibility issues arising between externally applied coatings and the native substrate matrix during conductive modification processes were critically examined. The article also identifies challenges that silk fibroin-based smart wearable devices currently face and suggests potential future development directions, providing theoretical guidance and a technical framework for the functional integration and performance optimization of silk fibroin-based smart wearable devices. Full article

Urban forests have a definition that has developed over time. Initially defined as urban greenery or as a measure of human impacts from urbanisation on forest systems, urban forests have varying definitions and are more often referred to for urban greenery. This urban greenery and measures of outcomes in sustainability terms are in urban landscapes and surroundings. With more specific definitions according to forest system definitions the complexity, multiple functions and advanced outcomes and functions of urban forest systems compared to other urban green space (UGS) types is more clearly understood. This article, using a literature review, discusses the definition of urban forests influencing how their impacts are measured, expected, and optimised. With clarified definitions, urban forest quality is considered in the literature review by search terms and topics of selected articles. Examples of selected indicators of the quality of urban forests and then of software and metrics used to plan and design urban greenery are presented. Refined wilding as a concept for urban functional biodiversity is then compared and used as a conceptual frame to analyse findings and prove the relevance and contribution of knowledge of the concept itself. Indicators of measures are provided, and they lead to a suggestion for clearer defining of urban forests. The findings can influence planning, design, implementation, and evaluation of urban forests as a higher-quality UGS type with multiple functions. Urban forests require improved defining of the value, quality, and coverage of their UGS type to be optimised. Refined wilding can give conceptual guidance for understanding the multiple and advanced functions that urban forest biodiversity provides for urban landscapes and populations. Urban tree canopy and urban forest systems in an urban landscape, as compared to other UGSs that connect to forested areas, either urban or peri-urban, are important differentiating definitional factors. Different metrics encourage a measure of this difference. The human realities of an urban landscape and population will determine whether and how a forest system can exist in a suburban landscape and are influential as to whether an urban tree canopy compared to a multifunctional diverse stratified semi-natural system of wild native and non-native varieties is established and can be maintained. The importance of maintaining newly established and existing urban forests and trees is a significant factor. Full article

This study investigated the chemical, thermal, and mechanical properties of segmented polyurethanes (SPUs) synthesized using less common biodegradable polyester polyols, specifically poly(adipate) (PAD) and poly(sebacate) (PSC), to evaluate their potential as nerve guidance conduits (NGCs) in peripheral nerve regeneration. The synthesis of novel 4,4′ methylene-bis-cyclohexyl diisocyanate (HMDI) SPUs was conducted in a two-step process: prepolymer formation and chain extension with 1,4-butanediol (BO) or 1,4-butanediamine (BA). SPUs were synthesized with two molar ratios—polyol:HMDI:BA/BO at 1:2:1 and 1:3:2 for the PAD:HMDI:BA system—to optimize mechanical properties. 1HRMN analysis verified the expected chemical structure of SPUs, whereas Raman and IR spectroscopy confirmed successful polyurethane synthesis. X-ray diffractograms showed that PAD-based SPUs (SPUPAD) were amorphous while PSC-based SPUs (SPUPSC) exhibited semi-crystalline behavior. SPUPAD showed only one degradation stage by TGA, while DSC showed one thermal event. In contrast, SPUPSC exhibited two degradation stages and three thermal events that confirmed phase separation. The longitudinal tensile properties of an NGC fabricated from SPUA-PAD-2 (PAD:HMDI:BA (1:3:2)) after 30 days of immersion in water (25 °C) showed a lower modulus (4.46 ± 0.5 MPa) than native intact nerves (15.87 ± 2.21 MPa) but a similar modulus to extracted nerves (8.19 ± 7.27 MPa). This system exhibited a longitudinal tensile force of 11.1 ± 1.6 N, which is lower than that of peripheral nerves (19.85 ± 7.21 N) but higher than that of commercial collagen-based nerve guide conduits (6.89 ± 2.6 N). The observed properties suggest that PUA-PAD-2 has potential as a biomaterial for nerve regeneration applications. Full article

Global agricultural systems are predominantly concentrated in regions characterized by fertile soils, abundant precipitation, and gentle slopes. However, a significant proportion of farmland is situated in areas with poor soil quality, arid conditions, and steep slopes. In such challenging environments, particularly sandy-arid sloping farmlands, selecting native crops that are well-adapted to local conditions is critical for sustainable agricultural practices. This study categorizes local crops in arid regions into four distinct types: tall-stem monocotyledonous plants (represented by maize, Zea mays L.), short-stem monocotyledonous plants (represented by millet, Setaria italica), tap-rooted dicotyledonous plants (represented by soybean, Glycine max (L.) Merr.), and tuberous dicotyledonous plants (represented by potato, Solanum tuberosum L.). A quantitative evaluation framework was developed using five key indices: nitrogen fixation, anti-wind erosion, roots reinforcement, anti-water erosion, and water conservation. These indices were used to calculate the suitability index values for each crop type. The findings revealed that in sandy-arid sloping farmland regions, maize and millet emerged as the most suitable crops for cultivation, followed by soybean, while potato was identified as the least suitable. Maize exhibited high values across all five indices, particularly demonstrating exceptional performance in nitrogen fixation. Additionally, the study demonstrated that traditional farming practices are highly effective in sloping farmlands, since they not only promote crop growth but also mitigate soil erosion. This research offers insights into agricultural management in regions affected by drought, soil erosion, and steep terrain. The results highlight the feasibility of employing traditional farming methods to cultivate maize in such challenging environments, providing practical guidance for sustainable agricultural development. Full article

Tamarix chinensis Lour. is a halophytic shrub native to coastal China, commonly used in afforestation and ecological restoration due to its high tolerance to salinity and drought. To understand how this species maintains genetic variation and adapts to extreme environments, we examined the genetic diversity, mating system, and spatial genetic structure of a natural T. chinensis population on the geographically isolated and environmentally harsh Wudi Seashell Island. Using both SSR and ISSR markers, we observed high levels of genetic diversity despite the small population size and spatial fragmentation. SSR markers revealed an average of 11.75 alleles per locus, with an expected heterozygosity (He) of 0.754 and an observed heterozygosity (Ho) of 0.702. ISSR markers showed a polymorphic locus rate of 97.87%, with a mean He of 0.402. Parentage analysis revealed relatively long seed and pollen dispersal distances, with most dispersal occurring within 150 m and seeds and pollens occasionally reaching 948 m and 447 m, respectively. The species exhibited a mixed mating system, with a multilocus outcrossing rate of 0.554, contributing to gene flow and reducing inbreeding. A fine-scale spatial genetic structure was detected within 75 m, consistent across both SSR and ISSR markers, suggesting limited local gene dispersal. These findings provide new insights into the adaptive strategies of T. chinensis in marginal habitats and offer valuable guidance for conservation and restoration efforts in vulnerable coastal ecosystems. Full article

ATP analogues are essential tools in enzymology and structural biology, but the structural and functional implications of their chemical modifications on nucleotide-binding proteins are often underappreciated. To address this, we evaluated a panel of ATP analogues, focusing on thiosubstituted and fluorinated molecules, using the AAA+ ATPase p97 as a benchmark system. Hydrolysis stability and impact on protein conformation, binding modes, and kinetics of enzymatic catalysis were assessed by protein-detected methyl NMR and ligand-detected ¹⁹F NMR in solution, as well as ³¹P solid-state NMR of nucleotides within protein sediments. ATPγS and AMP-PNP emerged as the most suitable analogues for preserving pre-hydrolysis states over extended periods, despite undergoing gradual hydrolysis. In contrast, both AMP-PCP and α/β-thiosubstituted analogues failed to induce native protein conformations in p97. Notably, we demonstrate a novel real-time NMR setup to explore the effect of nucleotide mixtures on cooperativity and the regulation of enzymes. Additionally, aromatic fluorine TROSY-based ¹⁹F NMR shows promise for direct ligand detection in solution, even in the context of large macromolecular complexes. These findings provide critical guidance for selecting ATP analogues in functional and structural studies of nucleotide-binding proteins. Full article

High-quality 2-inch aluminum nitride (AlN) crystals were grown using a double-zone resistance heating system, and the growth mechanism of AlN bulk crystals was further investigated. It was found that during the growth process, the vapor pressure at the growth interface, as well as the quality and structure of the seed crystal, was closely related to the growth conditions. The 2-inch AlN crystals were characterized using high-resolution X-ray diffraction (HRXRD) and optical microscopy. Optical microscopy observations of different regions on the native surface of the crystals revealed several morphologies, including regular step flow, irregular step flow, and domain-like structures. Comparisons showed that areas of the crystal surface with regular step-flow morphology exhibited high crystal quality, whereas the crystal quality decreased progressively as the step-flow morphology diminished. Therefore, the crystal quality can be preliminarily assessed through the surface morphology, providing guidance for improving the crystal growth process. Full article

Head and neck squamous cell carcinoma (HNSCC) presents significant challenges in oncology due to its complex biology and poor prognosis. Traditional two-dimensional (2D) cell culture models cannot replicate the intricate tumor microenvironment, limiting their usefulness in studying disease mechanisms and testing therapies. In contrast, three-dimensional (3D) in vitro models provide more realistic platforms that better mimic the architecture, mechanical features, and cellular interactions of HNSCC. This review explores the mechanical properties of 3D in vitro models developed for HNSCC research. It highlights key 3D culture techniques, such as spheroids, organoids, and bioprinted tissues, emphasizing their ability to simulate critical tumor characteristics like hypoxia, drug resistance, and metastasis. Particular attention is given to stiffness, elasticity, and dynamic behavior, highlighting how these models emulate native tumor tissues. By enhancing the physiological relevance of in vitro studies, 3D models offer significant potential to revolutionize HNSCC research and facilitate the development of effective, personalized therapeutic strategies. This review bridges the gap between preclinical and clinical applications by summarizing the mechanical properties of 3D models and providing guidance for developing systems that replicate both biological and mechanical characteristics of tumor tissues, advancing innovation in cancer research and therapy. Full article

The blackberry poses a threat as an invasive plant in various regions worldwide, where it aggressively competes with native species and risks delicate ecosystems. Livestock grazing has emerged as a potential strategy to mitigate its spread. This study investigated the effects of seasonal variations and altitude on the chemical composition and in vitro degradability of blackberry leaves. The leaves accessible to goats were collected in the Northwestern Italian Alps across all seasons at three altitudes (low: 450 m, medium: 700 m, high: 1000 m). The findings indicated that blackberry leaves can serve as a cost-effective, high-protein, and high-fiber feed for ruminants in these regions. However, the goats exhibited a limited capacity for fiber degradation. Furthermore, the nutritional value of these leaves was significantly influenced by the season, altitude, and their interaction. Spring leaves had the highest protein content (241.9 g/kg dry matter) and the best digestibility, with lower lignin levels (69 g/kg dry matter). At higher altitudes, lignin content decreased significantly during winter compared to the other seasons, resulting in a significant increase in fiber degradability. These insights offer crucial guidance for optimizing the utilization of the blackberry plant in goat feeding systems and underscores the necessity of considering both seasonal and altitudinal factors in grazing management practices. Full article

AI/AN communities are disproportionately impacted by food insecurity and gestational diabetes mellitus (GDM). Decreasing the risk of GDM can interrupt the intergenerational cycle of diabetes in AI/AN families, and can decrease diabetes-related health disparities. The goal of this study was to explore ways of supporting holistic health and reducing the risk of GDM among young American Indian and Alaska Native (AI/AN) females prior to pregnancy. Semi-structured interviews were conducted with adult AI/AN women (>18 years) who had GDM or who have young female relatives (e.g., daughters) (n = 41), AI/AN females between 12 and 24 years (n = 18), and key experts in food/nutrition and health within AI/AN communities (n = 32). Three themes emerged: (1) guidance on how to support young females’ holistic wellness; (2) evidence that generations of colonial violence, including forced removal, forced poverty, and the imposition of a Western-based food system, causes deeper, systemic fracturing of traditional cultural food knowledge and practices; and the fact that (3) opportunities for improved adolescent female health are rooted in AI/AN values and how AI/AN people resist the impacts of anti-Indigenous racism through family-based, community-led, and holistic health. These themes suggest alternative understandings about the relationships between food insecurity and holistic pre-pregnancy health and can guide our next steps in decreasing health disparities in these communities. Full article

Many emerging applications, such as factory automation, electric power distribution, and intelligent transportation systems, require multicast Ultra-Reliable Low-Latency Communications (mURLLC). Since 3GPP Release 17, 5G systems natively support multicast functionality, including multicast Hybrid Automatic Repeat Request and various feedback schemes. Although these features can be promising for mURLLC, the specifications and existing studies fall short in offering guidance on their efficient usage. This paper presents the first comprehensive system-level evaluation of mURLLC, leveraging insights from 3GPP specifications. It points out (i) how mURLLC differs from traditional multicast broadband wireless communications, and (ii) which approaches to provide mURLLC require changing the paradigm compared with the existing solutions. Finally, the paper provides recommendations on how to satisfy strict mURLLC requirements efficiently, i.e., with low channel resource consumption, which increases the capacity of 5G systems for mURLLC. Simulation results show that proper configuration of multicast mechanisms and the corresponding algorithms for mURLLC traffic can reduce resource consumption up to three times compared to the baseline solutions proposed for broadband multicast traffic, which significantly increases the system capacity. Full article

The legacy effects of invasive plant species can hinder the recovery of native communities, especially under nitrogen deposition conditions, where invasive species show growth advantages and trigger secondary invasions in controlled areas. Therefore, it is crucial to thoroughly investigate the effects of nitrogen deposition on the legacy effects of plant invasions and their mechanisms. The hypotheses of this study are as follows: (1) Nitrogen deposition amplifies the legacy effects of plant invasion. This phenomenon was investigated by analysing four potential mechanisms covering community system structure, nitrogen metabolism, geochemical cycles, and microbial mechanisms. The results suggest that microorganisms drive plant–soil feedback processes, even regulating or limiting other factors. (2) The impact of nitrogen deposition on the legacy effects of plant invasions may be intensified primarily through enhanced nitrogen metabolism via microbial anaerobes bacteria. Essential insights into invasion ecology and ecological management have been provided by analysing how nitrogen-fixing bacteria improve nitrogen metabolism and establish sustainable methods for controlling invasive plant species. This in-depth study contributes to our better understanding of the lasting effects of plant invasions on ecosystems and provides valuable guidance for future ecological management. Full article

Intention detection and slot filling are two major subtasks in building a spoken language understanding (SLU) system. These two tasks are closely related to each other, and information from one will influence the other, establishing a bidirectional contributory relationship. Existing studies have typically modeled the two-way connection between these two tasks simultaneously in a unified framework. However, these studies have merely contributed to the research direction of fully using the correlations between feature information of the two tasks, without sufficient focusing on and utilizing native textual semantics. In this article, we propose a semantic guidance (SG) framework, enabling enhancing the understanding of textual semantics by dynamically gating the information from both tasks to acquire semantic features, ultimately leading to higher joint task accuracy. Experimental results on two widely used public datasets show that our model achieves state-of-the-art performance. Full article

Leveraging technological advancements such as containers, microservices, and service mesh, cloud-native edge computing (CNEC) has become extensively discussed and applied in both academia and industry. The integration of mobile edge computing and communication is crucial for the future communication architecture in order to fully utilize distributed and fragmented communication resources and computing power. The potential for cloud-native integration can help merge mobile edge computing and communication, enhancing network flexibility and resource utilization. This paper investigates the implementation plan for extending cloud-native capabilities to integrated computing and communication (INCCOM) in the satellite–terrestrial network. We construct an experimental verification platform called ComEdge in a real-world setting. Subsequently, we analyze the architecture, functional characteristics, and deployment of the platform in a real-world environment. Furthermore, we explore the solution of deep reinforcement learning in the deployment of cloud-native core network and conduct a preliminary verification of the platform’s potential to enable artificial intelligence in a real production environment, which will provide guidance to both academic and industry sectors. Finally, we conduct an analysis on the challenges and opportunities encountered by the cloud-native INCCOM network system. Full article

Injuries to the peripheral nervous system are a common clinical issue, causing dysfunctions of the motor and sensory systems. Surgical interventions such as nerve autografting are necessary to repair damaged nerves. Even with autografting, i.e., the gold standard, malfunctioning and mismatches between the injured and donor nerves often lead to unwanted failure. Thus, there is an urgent need for a new intervention in clinical practice to achieve full functional recovery. Nerve guidance conduits (NGCs), providing physicochemical cues to guide neural regeneration, have great potential for the clinical regeneration of peripheral nerves. Typically, NGCs are tubular structures with various configurations to create a microenvironment that induces the oriented and accelerated growth of axons and promotes neuron cell migration and tissue maturation within the injured tissue. Once the native neural environment is better understood, ideal NGCs should maximally recapitulate those key physiological attributes for better neural regeneration. Indeed, NGC design has evolved from solely physical guidance to biochemical stimulation. NGC fabrication requires fundamental considerations of distinct nerve structures, the associated extracellular compositions (extracellular matrices, growth factors, and cytokines), cellular components, and advanced fabrication technologies that can mimic the structure and morphology of native extracellular matrices. Thus, this review mainly summarizes the recent advances in the state-of-the-art NGCs in terms of biomaterial innovations, structural design, and advanced fabrication technologies and provides an in-depth discussion of cellular responses (adhesion, spreading, and alignment) to such biomimetic cues for neural regeneration and repair. Full article