Search Results (683)

Non-textile application of silk fiber is the major focus of the present scientific communities. Characteristics, i.e., structural, mechanical, are the key advantages of silk protein to make it promising candidates for its variable application. Keeping this in view, the present investigation has been conducted to understand the structural and mechanical variability of silk breeds, i.e., CSR₂ × CSR₄ (single hybrid), PM × CSR₂ (cross breed) and FC₁ × FC₂ (double hybrid) for their respective promising non-textile application. It is envisaged that FC₁ × FC₂ (double hybrid) has the highest tensile strength (431.47 ± 28.46 MPa), Young’s modulus (5.92 ± 0.45 GPa) and β-sheet content (46.62 ± 1.45%). The lowest nano-crystallite size (3.34 ± 0.22) and elongation % (10.85 ± 0.77) were also observed in the FC₁ × FC₂. Further, significant positive correlation was observed between β-sheet with crystalline % (p *** < 0.001; r = 0.95), crystalline % with tensile strength (p *** < 0.001; r = 0.91) and Young’s modulus with tensile strength (p * < 0.001; r = 0.80). This indicates that the higher the β-sheet content is, the higher the tensile strength and higher crystalline phase of the fiber will be. Crystallite size has a negative correlation with the β-sheet content, crystalline %, tensile strength and Young’s modulus, which shows that the lower the crystallite size, the more the compactness and strength will be. Full article

Agricultural pests can rapidly adapt to chemical pressures, and expression-based surveys of chemosensory genes may not fully capture the associated genomic variation. We hypothesized that the molecular profiles of chemosensory and detoxification genes in the mulberry thrips Pseudodendrothrips mori Niwa (Thysanoptera: Thripidae) are associated with local genomic variability and methylation context alongside transcript abundance. To explore this, we integrated PacBio HiFi-derived single-nucleotide polymorphisms (SNPs), structural variants (SVs), DNA methylation, and RNA-seq data on a chromosome-level reference genome. We analyzed 179 focal genes from six families, applying a consensus prioritization framework—based on weighted percentiles of feature values, principal component distances, and anomaly-detection scores—to rank the candidates. The integrated priority score correlated positively with SNP (r = 0.603) and SV burden (r = 0.632) and negatively with local methylation (r = −0.524), whereas its correlation with expression was weaker (r = 0.427). Three OBPs—PSMOgene01223, PSMOgene012530, and PSMOgene012982—emerged among the highest-priority candidates, exhibiting favorable in silico docking scores (−5.038 to −6.792 kcal/mol) with (Z)-octadec-11-enyl acetate and a long-chain oxygenated acetate. These findings indicate potential linkages between multi-omics plasticity and chemosensory gene variation. Furthermore, these computationally prioritized OBPs suggest potential targets for exploring semiochemical-based management tools. Full article

Fibroin-based films represent a promising platform for sustainable and bio-derived materials. Existing literature has mainly focused on isolated molecules, plasticizers, or chemical cross-linkers, and the function of complex, multi-component natural extracts as structure-modulating agents in fibroin films remains poorly understood. In this study, edible films containing mulberry leaf extract (MLE; 2–8 wt%) and fibroin (8 wt%) were prepared by solution casting, and their structures were investigated using spectroscopic, morphological, thermal, mechanical, and barrier property analyses. The results reveal that MLE induces concentration-dependent changes in film performance through multicomponent, non-covalent interactions with the fibroin. An approximately 187% increase in tensile strength was achieved at high MLE concentration, confirming effective physical reinforcement. The water vapor transmission rate decreased markedly from 0.888 to 0.170 g·h⁻¹·m⁻², indicating an enhanced moisture barrier, whereas oxygen permeability increased at higher extract loadings, suggesting localized chain rearrangements. High optical transparency in the visible region was maintained (79.95–83.77%), while UV response was selectively altered with extract concentration. Overall, the 8MLE formulation exhibited the most balanced performance. This study demonstrates that plant-derived extracts can serve as effective natural modifiers for tailoring fibroin film properties without inducing crystallization, offering a sustainable strategy for designing bio-based and edible protein film systems. Full article

Host alternation is a common behavioral strategy among many herbivorous insects. The mulberry longhorn beetle, Apriona germari is a destructive wood-boring pest. The adults exhibit rhythmic host alternation between feeding and oviposition hosts. However, the temporal rhythm and regulatory mechanisms underlying this behavior remain unclear. In the present study, by observing the dynamics of the numbers of A. germari on mulberry (Morus alba, feeding host) and willow (Salix babylonica, oviposition host) trees in a cage, we first found that both females and males began to aggregate on mulberry trees at dawn. Following 18:00 at dusk, the number of females on mulberry declined sharply, whereas the number of males decreased slightly, with a greater proportion of males staying on mulberry. To investigate the role of host volatiles in regulating the host alternation in A. germari, we then carried out two-choice olfactory assays to test whether the behavioral responses of A. germari to the host volatiles from mulberry and willow twigs differed between 04:00 and 08:00 (dawn, aggregated on mulberry) and 18:00 to 22:00 (dusk, dispersed from mulberry). Males were consistently attracted to the volatiles from healthy mulberry at both dawn and dusk, but not to those from willow. Females showed no attraction to the volatiles from either of two hosts at dawn; however, at dusk, they were significantly attracted to willow volatiles. Furthermore, volatiles from feeding-damaged mulberry trees were repellent to both sexes, particularly to males at dawn. These results indicate that the rhythmic host alternation in A. germari is partly mediated by host volatiles and an unidentified male-produced pheromone may be present in this species. Our findings can deepen the understanding of the host alternation in longhorn beetles, and offer a theoretical foundation for developing semiochemical-based, eco-friendly strategies for controlling this pest. Full article

Background/Objectives: Resveratrol is a naturally occurring polyphenolic stilbene compound exhibiting a wide range of biological activities, and it has been extensively utilized as both a food additive and a pharmaceutical active ingredient. Typically, it can be directly extracted from natural sources such as grapes, mulberries, and peanuts, or obtained through catalytic hydrolysis of polydatin. To establish an efficient and optimized method for resveratrol production, we conducted a comprehensive study to refine the acid-catalyzed hydrolysis conditions of polydatin. Methods: A high-performance liquid chromatography method was developed for the quantitative determination of polydatin and resveratrol. To identify the optimal ranges of reaction temperature, HCl concentration, and ethanol concentration, single-factor experiments were conducted by evaluating their influences on hydrolysis kinetics and resveratrol yield. Based on these results, response surface methodology incorporating a Box–Behnken design was employed to optimize the hydrolysis process, using resveratrol yield as the response variable. Furthermore, time-course experiments were performed to determine the optimal reaction duration under the established optimal conditions. Results: Single-factor experiments demonstrated that increasing temperature and HCl concentration significantly accelerated hydrolysis, but resveratrol yield increased initially and then decreased with excessive increases in either factor. To further optimize the process, response surface methodology optimization experiments were conducted at temperatures of 60, 70, and 80 °C; HCl concentrations of 1.0, 1.5, and 2.0 M; and ethanol concentrations of 75%, 85%, and 95%. The optimal conditions were identified as follows: temperature, 70 °C; HCl concentration, 1.5 M; ethanol volume fraction 85%; and reaction time, 5 h. Under these conditions, the theoretical resveratrol yield was 85.68%, and the average yield from triplicate validation experiments was 86.01% (RSD = 0.56%), which was consistent with the theoretical value. Conclusions: The optimized acid-catalytic hydrolysis process using RSM is stable, feasible, and efficient, offering a promising approach for enhancing resveratrol production from polydatin. Full article

The selective harvesting of leaves from flexible-stem crops remains a major challenge in agricultural mechanization due to stem compliance, heterogeneous petiole strength, and unstable tool–crop interaction. To address these issues, a bio-inspired ring-cutting and compliant clamping harvesting mechanism is proposed for low-damage selective harvesting under complex terrain conditions. Inspired by the adaptive attachment behavior of octopus suckers, a flexible compliant clamping interface combined with a ring-shaped sliding cutting structure was developed to stabilize flexible stems during harvesting. A coupled kinematic–force analytical model was established to characterize the interaction between tool motion, stem feeding, and cutting behavior. In addition, a sliding cutting mechanics model was introduced to analyze the relationship between cutting force and sliding angle. Dynamic multibody simulations were performed using ADAMS to verify the motion feasibility and trajectory stability of the proposed harvesting mechanism. Bench-scale experiments were conducted using mulberry branches as a representative flexible-stem crop, and a response surface methodology based on a Box–Behnken experimental design was applied to optimize key operational parameters. The optimal parameter combination included a chain linear speed of 0.18 m·s⁻¹, a feeding speed of 0.30 m·s⁻¹, and an installation angle of 36°. Under these conditions, the missed harvest rate was reduced to 9.2–9.8%, demonstrating improved harvesting stability compared with conventional rigid cutting mechanisms. The results indicate that integrating compliant stabilization with sliding cutting provides an effective engineering strategy for selective harvesting of flexible-stem crops in complex agricultural environments. Full article

This review systematically summarizes the history of and recent progress in mulberry research, with particular emphasis on advances in mulberry genomics and its role in modern breeding and the circular bioeconomy. Studies on whole-genome sequencing, high-density genetic mapping, and comparative genomics are reviewed. In addition, strategies for identifying functional genes associated with stress resistance, quality traits, and metabolic pathways are summarized. Building on these advances, the applications of genome editing, marker-assisted selection, and multi-omics-integrated breeding for improving stress resistance in mulberry are discussed. Current evidence indicates that developments in genomics have significantly shortened the mulberry breeding cycle, improved selection precision, and enhanced breeding efficiency while also providing molecular support for the development of a mulberry-based circular bioeconomy chain. In recent years, substantial progress has been achieved in mulberry genomics research. However, as an allopolyploid plant, the complexity of the mulberry genome continues to pose challenges for in-depth genomic analysis. Current limitations include incomplete reference genomes, insufficient functional annotation, and complex genetic backgrounds that hinder further genomic dissection. To address these challenges, strategies such as multi-omics integration, optimization of emerging genome-editing technologies, and diversified application models are proposed. These approaches aim to promote high-quality precision breeding and strengthen the integration of mulberry improvement with the circular bioeconomy, thereby maximizing the utilization and application value of mulberry resources. Full article

The efficiency of six deep eutectic solvents (DESs) based on choline chloride (ChCl) and various hydrogen bond donors (HBDs) was evaluated against a traditional organic solvent for extracting polyphenolic bioactive compounds from three different white mulberry samples (Morus alba), including branches, leaves, and fruits. Ultrasound-assisted extraction was performed under selected conditions identified for ChCl/glycerol DES: a 1:2 molar ratio of hydrogen bond acceptor to HBD, 20% water added to the DES, a temperature of 80 °C, and an extraction time of 30 min, providing a set of standard parameters for comparing the efficiency of different DESs. Extraction efficiencies were assessed using a developed and validated HPLC method, as well as total phenolic content and total flavonoid content assays. Among the tested DESs, those composed of ChCl and polyalcohols as HBDs showed the best performance. For branch and leaf samples, the ChCl/glycerol DES was the most effective, while for fruit samples, the ChCl/ethylene glycol DES showed the highest efficiency. In most polyphenol extractions tested, at least one DES achieved extraction efficiencies comparable to or higher than those obtained with methanol, except for flavonoids, for which DES yields were often lower. Overall, the results indicate that using DESs represents a greener and more sustainable approach to extracting bioactive compounds from white mulberry. Full article

The effects of substituting pelleted diets manufactured from cassava, chaya, and mulberry leaves for concentrate on growth performance, feed intake, rumen fermentation, and microbial protein synthesis in beef cattle were evaluated. Four beef cattle (initial BW: 250 ± 50 kg) were assigned to four treatments: a control diet (T1) and diets in which 50% of the concentrate was replaced with cassava leaf pellets (T2), chaya leaf pellets (T3), or mulberry leaf pellets (T4). The data were analyzed using a 4 × 4 Latin square with animal as a period effect as appropriate. Rumen volatile fatty acids were determined by means of HPLC, and microbial protein synthesis was assessed using urinary purine derivatives. Cattle fed cassava leaf pellets (T2) showed the greatest average daily gain (0.79 kg/d) compared with the control (0.50 kg/d; p < 0.05). Compared with T1, T4 exhibited a higher ruminal propionate proportion and total VFA concentration, which was associated with a lower acetate-to-propionate pattern, suggesting reduced methanogenic potential. No adverse health effects were observed, as indicated by hematocrit and blood urea nitrogen values within normal ranges. Microbial protein production increased in the leaf-pellet treatments, with T4 showing the highest efficiency. Overall, cassava, chaya, and mulberry leaf pellets can partially replace concentrate while maintaining growth performance and improving rumen fermentation efficiency in beef cattle. Full article

Drought stress severely limits the growth and productivity of Morus alba, yet the molecular mechanisms underlying its adaptation remain poorly understood. Trehalose, an important osmoprotectant and signaling molecule, plays a key role in plant responses to abiotic stress, and its biosynthesis is primarily regulated by trehalose-6-phosphate synthase (TPS). However, the characteristics and potential functions of TPS genes in M. alba have not been systematically investigated. In this study, we identified 11 TPS genes (MaTPSs) in the M. alba genome and performed comprehensive analyses, including phylogenetic relationships, gene structures, conserved motifs, cis-regulatory elements, and expression profiles. Phylogenetic analysis classified MaTPSs into TPS I and TPS II subfamilies, with closer evolutionary relationships to Populus trichocarpa than to Arabidopsis thaliana. Promoter analysis revealed the presence of multiple stress- and hormone-responsive elements, suggesting their potential involvement in abiotic stress regulation. Physiological measurements showed that drought stress significantly increased trehalose accumulation, with a 1.6-fold increase in leaves and a 2.2-fold increase in roots. Expression profiling further demonstrated that six MaTPS genes were upregulated under drought stress, among which MaTPS4, MaTPS9, MaTPS10, and MaTPS11 exhibited significant induction (approximately 5-, 5-, 8-, and 10-fold, respectively). Correlation analysis further indicated that trehalose accumulation was positively associated with all upregulated MaTPS genes (p < 0.05). Taken together, these results suggest that MaTPS genes may be involved in drought-responsive regulation of trehalose metabolism in M. alba. This study provides a valuable foundation for future functional validation and the genetic improvement of drought tolerance in mulberry. Full article

This study evaluates the ability of three classification procedures to distinguish areas with different levels of atmospheric pollution, based on biomonitoring carried out by analyzing the color and spectral characteristics of mulberry (Morus L.) and linden (Tilia L.) leaves. Sampling was carried out in areas that were grouped into four classes according to the concentrations of fine particulate matter (PM2.5, PM10) and gaseous pollutants (TVOC, NOx, SOx, CO, and eCO₂), measured using a specialized multisensor device. A total of 57 informative features were analyzed, representing indices obtained from two color models (RGB and Lab), as well as from VIS and NIR spectral characteristics measured for the adaxial and abaxial leaf surfaces. The data processing methodology includes feature selection using the ReliefF method and a comparative analysis between two approaches to dimensionality reduction—principal components (PC) and latent variables (LV). The results indicate that data reduction using PC provides significantly higher accuracy and better class separability, regardless of the classifier used, compared to LV, where errors exceed 40%. The comparison between classifiers shows a clear superiority of nonlinear models. While linear discriminant analysis demonstrates low efficiency, quadratic discriminant analysis (Q and DQ) and SVM with radial basis function (RBF) achieve high accuracy of class separability, reaching 100% in the SVM-RBF model for both tree species. The study also reveals functional asymmetry: the adaxial side of the leaves is more informative for spectral indices, while the abaxial side is more sensitive to color changes. The results confirm that the combined optical characteristics obtained from the leaf surface of bioindicators form a reliable method for ecological monitoring of air quality in urban areas. Full article

Mulberry is a plant species of significant economic value and is widely incorporated into various traditional medicinal formulations. Its multiple botanical parts (leaves, branches, fruits, seeds, and roots) possess both nutritional and therapeutic properties. Throughout its growth cycle, mulberry is exposed to a range of abiotic and biotic stresses. In response, the plant has evolved a suite of stress tolerance mechanisms, notably including the synthesis of diverse secondary metabolites. These metabolites, which encompass phenolic acids, flavonoids, and volatile aromatic compounds, exhibit pronounced pharmacological activities. This review systematically elucidates the roles of mulberry-derived phenolic compounds, alkaloids, and polysaccharides, which demonstrate a broad spectrum of biological effects, including antioxidant, antibacterial, antiviral, anticancer, anti-inflammatory, neuroprotective, anti-obesity, antidiabetic, and anti-enteritis activities. By integrating knowledge of mulberry’s adaptive mechanisms to abiotic and biotic stresses with the therapeutic functions of its extracts, this review aims to provide novel insights to guide future molecular breeding strategies and drug development efforts. Full article

Mulberry (Morus alba L.) is a woody plant primarily cultivated for silkworm breeding, with significant economic and ecological functions. Its nitrogen use efficiency directly affects leaf yield, quality, and environmental adaptability. The main inorganic nitrogen forms available for plant uptake in soil are ammonium nitrogen and nitrate nitrogen, and plant uptake and assimilation of these two nitrogen sources often exhibit species-specific preferences. This review systematically summarizes the research progress on nitrogen uptake preferences in mulberry, confirming that this species generally shows a preferential uptake of nitrate. Specifically, when supplied with nitrate or a mixed nitrogen source dominated by nitrate, mulberry exhibits better performance in growth and development, photosynthetic efficiency, and accumulation of secondary metabolites. This review further discusses the physiological characteristics and underlying regulatory mechanisms responsible for this preference, and analyzes key factors affecting nitrogen uptake preferences, including soil properties, environmental stresses, and microbial interactions. It should be noted that while controlled experiments have yielded important insights, the applicability of these findings under complex field conditions still requires further validation through field trials. Finally, future research directions are prospected, including in-depth dissection of molecular mechanisms, field validation, plant-microbe interactions, and nutritional strategies for stress resistance, aiming to provide a theoretical basis for efficient cultivation and precise nitrogen management of mulberry. Full article

The silkworm, Bombyx mori (Bm), is an insect that contributes to industries such as silk production, cosmetics, medicine, and food, as well as to scientific research. A previous study showed that Bm odorant receptor (BmOr) genes, BmOr44, BmOr54, and BmOr63, may play a major role in oviposition. This research aimed to investigate the function of these three genes using a double-stranded RNA (dsRNA) technique to knock down their expression levels. Our results revealed that the gene-specific dsRNAs could moderately reduce the expression levels of BmOr44, BmOr54, and BmOr63 in the silk moth antenna. Silk moths were injected with 50 nM dsRNABmOr54 and 100 nM dsRNABmOr63 and showed relative oviposition rates under the mulberry leaves condition at 111.45% and 109.58%, respectively, when compared to those with dsRNAlacZ injection. The reduction in expression levels of these three genes showed no effect on the oviposition rates of the silk moths without mulberry leaves treatment. The expression levels of these BmOr genes were restored after fertilization, suggesting the temporary effects of the dsRNAs. Our findings suggested that variation in BmOr54 expression level was correlated with changes in oviposition behavior in Bombyx mori. Full article

Mulberry (Morus alba) is an economically important forest tree species, yet cutting propagation is constrained by low adventitious rooting efficiency. Although ABT, a composite rooting promoter, can improve cutting survival, its molecular basis remains unclear. Here, cuttings of the cultivar Qiangsang 1 were treated with ABT, NAA, or IAA (200–1000 mg/L) and subjected to transcriptome profiling to elucidate how ABT enhances rooting. Hormone-related analyses showed that ABT upregulated GH3 (auxin-amido synthetase) at days 0 and 20, implicating auxin homeostasis. ERF1/2 (ethylene response factors) exhibited a temporal oscillation, with induction at day 10 followed by repression from days 20 to 30, consistent with a shift from developmental programs to defense-related processes. In parallel, JAZ (jasmonate ZIM-domain) genes were downregulated at day 0 and subsequently upregulated; together with CYP94C1, these changes may attenuate jasmonate-associated defense signaling. For cell remodeling and defense coordination, ABT reduced the expression of genes associated with cell-wall rigidity while inducing EXPA11 (expansin) at day 20, potentially facilitating root primordium emergence. Meanwhile, PR-1 (pathogenesis-related protein 1) was transiently upregulated at days 0, 20, and 30, and the concomitant modulation of WRKY transcription factors and RPM1 suggests enhanced defense readiness. Integrative network analysis further indicated that a GH3–ERF1/2–PR-1 module links hormonal and defense cues and may activate BAT1 (energy metabolism) and RBOHB (ROS production) to support adventitious root elongation. Collectively, these results suggest that ABT improves rooting efficiency by reshaping hormonal homeostasis and coordinating cell-wall reconstruction with a pre-activated defense state, thereby providing a conceptual framework for balancing root induction and defense responses during vegetative propagation in forest trees. Full article