Search Results (8,092)

Multi-axle electric heavy-duty trucks face significant challenges in maintaining braking stability and achieving real-time control during regenerative braking due to their large mass and complex inter-axle coupling dynamics. To address these issues, this paper proposes an improved model predictive control (IMPC) strategy that enhances computational efficiency and control responsiveness through an instantaneous response mechanism. The approach integrates a first-order error attenuation term within the MPC framework and employs an extended Kalman filter to estimate tire–road friction in real time, enabling adaptive adjustment between energy recovery and stability objectives under varying road conditions. A control barrier function constraint is further introduced to ensure smooth and safe regenerative braking. Simulation results demonstrate improved energy recovery efficiency and faster convergence, while real-vehicle tests confirm that the IMPC maintains superior real-time performance and adaptability under complex operating conditions, reducing average computation time by approximately 14% compared with conventional MPC and showing strong potential for practical deployment. Full article

Background: The high prevalence of overweight and obesity in Singapore necessitates scalable primary prevention strategies. This study evaluated the short-term effectiveness of FitterLife, a 12-week, digitally delivered, group-based behavioural weight management programme targeting at-risk adults without diabetes or hypertension in the community. Methods: In a retrospective matched cohort study, we compared 306 FitterLife participants (enrolled from October 2021 to January 2025) with 5087 controls identified from a population health data mart, matched on age, sex, ethnicity, and baseline body mass index (BMI). The primary outcome was achieving ≥5% weight loss or a ≥1 kg/m² BMI reduction at 12 weeks. Programme effectiveness was analysed using propensity score matching (1:1) and inverse probability weighted regression. Mixed-effects models assessed weight/BMI trajectories and modified Poisson regression identified behavioural factors associated with success. Results: After matching, FitterLife participants were more likely to achieve the weight loss target than controls (45.7% vs. 13.7%, coefficient = 0.32, 95% confidence interval [CI]: 0.26–0.38) and were over three times as likely to succeed (Adjusted incidence rate ratio [aIRR] = 3.37, 95% CI: 2.87–3.93). The programme group showed significant reductions in weight (−2.23 kg, 95% CI: −2.57 to −1.90) and BMI (−0.86 kg/m², 95% CI: −0.95 to −0.73) at the end of programme. Higher session attendance and improved behavioural factors were associated with success. Conclusions: FitterLife was effective in achieving clinically significant short-term weight loss in a real-world setting. The findings demonstrate the potential of a scalable, behavioural theory-informed, virtual group model as a viable primary prevention strategy within national chronic disease management efforts. Full article

Accurate prediction of urban air quality during fire events is frequently limited by the lack of experimentally validated source terms. Current pollution dispersion models often rely on idealized input parameters that fail to capture the complex thermal and chemical dynamics of real-world indoor fires. Addressing this gap, this study presents an experiment which reproduces an office-type fire using a wooden crib with a calibrated thermal load, enabling accurate determination of the HRR through the mass loss rate method. Temperature evolution was monitored with 27 thermocouples positioned across five vertical planes, while toxic gas concentrations were measured near the upper boundary of the exterior opening. The experimental data were used to calibrate and validate the numerical model, with HRR representing the primary parameter and temperature and toxic gas concentrations serving secondary validation metrics. Quantitative comparison showed good agreement between the experimental and simulated trends, with acceptable prediction errors and consistent reproduction of the dominant physical mechanisms governing fire development, ventilation-driven flow, and pollutant accumulation. Overall, the combined experimental–numerical approach provides a validated framework for future simulations on larger and more complex geometries, ensuring reliable input parameters for urban-scale fire scenarios. Full article

Decreased skeletal muscle mass and function are a serious complication of long-term diabetes, often leading to numerous adverse outcomes. The primary pathological features of diabetic sarcopenia include muscle fiber atrophy and interstitial fibrosis. Although resistance exercise (RE) has been reported to mitigate skeletal muscle atrophy in type 2 diabetes mellitus (T2DM), the underlying mechanisms remain unclear. Fibroblast growth factor 21 (FGF21), an exercise-induced cytokine, has been shown to protect against skeletal muscle atrophy at elevated levels. In this study, a T2DM mouse model was established through 12 weeks of high-fat diet feeding and intraperitoneal injection of streptozotocin (STZ) to investigate the effect and mechanism of RE on skeletal muscle atrophy in T2DM mice. Our results demonstrated that 8 weeks of RE significantly decreased body weight, fat mass, triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG), and serum insulin levels in T2DM mice. RE also improved lean mass, glucose tolerance (IPGTT), and insulin tolerance (ITT). Additionally, RE increased skeletal muscle mass cross-sectional area (CSA) while attenuating fibrosis and inflammatory responses in skeletal muscle. Notably, RE upregulated FGF21 expression and activated the PI3K/Akt signaling pathway in diabetic skeletal muscle. RE promoted the phosphorylation of mTOR, 4EBP1, and p70S6K while suppressing the expression of the atrophy-related E3 ubiquitin ligases MuRF1 and MAFbx/Atrogin-1. Furthermore, RE inhibited lipid synthesis and enhanced both lipid oxidation and glucose utilization in skeletal muscle of T2DM mice. RE also improved mitochondrial biogenesis and dynamics in skeletal muscle of T2DM mice. In summary, 8 weeks of RE alleviated skeletal muscle atrophy in T2DM mice via activation of the FGF21/PI3K/Akt signaling pathway, which enhanced protein synthesis, improved glycolipid metabolism and mitochondrial quality control, and attenuated fibrosis and inflammation. Full article

Background: Rapid weight loss (RWL) is a widespread practice among judo athletes seeking to compete in lower weight categories. Restrictive nutritional and behavioral strategies used to achieve short-term reductions in body mass may impair physiological and psychological functioning. This study aimed to assess dietary behaviors, weight-cutting methods, and short-term physical and psychological effects of RWL among competitive judo athletes. Methods: A cross-sectional study was conducted between August and December 2024 among 70 judo athletes (17 women, 53 men) competing at national and international levels. Data were collected using an author-designed questionnaire addressing anthropometric variables, training history, RWL strategies, dietary restrictions, hydration manipulation, and post-weigh-in eating behaviors. Physical and psychological symptoms were assessed using frequency-based self-report measures. Results: Most athletes (95.7%) reported engaging in RWL, typically beginning between ages 13 and 16 years (65.7%). Athletes reduced an average of 3.64 ± 1.74 kg (2–5% of body mass). Common strategies included decreasing meal frequency (74.29%), increasing training load (72.86%), restricting fluids (68.57%), and using saunas (62.86%). Reported physical symptoms included weakness (71.43%), headaches (51.43%), and dizziness (45.71%), while psychological symptoms included irritability (57.14%), reduced motivation (40%), and tension (38.57%). Post-weigh-in binge eating occurred in 65.71% of athletes and was significantly associated with higher RWL magnitude (p < 0.05). Discomfort during competition related to overeating (55.71%) or insufficient intake (41.43%) was also frequent. Conclusions: RWL is highly prevalent among judo athletes and often begins early in training history. The strategies used are associated with adverse physical and psychological symptoms and maladaptive compensatory behaviors. These findings highlight the need for evidence-based nutritional education and individualized weight-management approaches to support athlete health and performance. Full article

The long-term stability of deep underground excavations near aquifer-bearing strata is primarily controlled by the time-dependent deformation and permeability changes in the surrounding rock mass under the combined effects of mechanical loading and groundwater seepage. This study experimentally investigates the creep behavior and permeability evolution of tuff specimens subjected to stepwise reductions in confining pressure under coupled seepage and stress conditions. Conventional triaxial compression tests were conducted to determine the peak strength at confining pressures of 10, 15, and 20 MPa. Subsequently, triaxial creep tests were performed, maintaining axial stress at 70% of the previously established peak strength, with a constant seepage pressure of 4 MPa, while progressively decreasing the confining pressure. The results clearly reveal a three-stage creep process—with instantaneous, steady-state, and accelerated phases—with the radial strain exceeding axial strain and ultimately dominating at failure. This indicates that failure is characterized by significant volumetric expansion. At the specified initial confining pressures of 10 MPa, 15 MPa, and 20 MPa, the tuff specimens exhibited volumetric strains of −1.332, −1.119, and −0.836 at failure, respectively. Permeability evolution depends on the creep stage, showing a pronounced increase during the accelerated creep phase that often surpasses the cumulative permeability changes observed earlier. The specimen’s permeability at failure increased by factors of 3.97, 3.21, and 3.61 compared to the initial stage of the experiment, respectively. Additionally, permeability evolution exhibits a strong functional relationship with volumetric strain, which can be effectively modeled using an exponential function. The experimental findings further indicate that, as the confining pressure is gradually reduced, the permeability evolves following a clear exponential trend. Additionally, a higher initial confining pressure slows the rate at which permeability increases. These findings clarify the three-stage creep behavior and the associated evolution of the permeability index in tuff under coupled seepage–stress conditions. Additionally, they present a quantitative model linking permeability to volumetric strain, offering both a theoretical foundation and a new approach for assessing the long-term stability risks of deep underground engineering projects. Full article

Most polymeric plastics used as food packaging are obtained from petroleum or made with non-biodegradable synthetic molecules, which slowly degrade and leach into the environment, resulting in the accumulation of microplastics along the trophic chains. To mitigate these impacts, biodegradable packaging derived from agro-industrial biomass residues has emerged as a promising alternative. In this study, bio-based methylcellulose films reinforced with cellulose nanocrystals (CNCs) extracted from low-quality coffee beans were developed and fully characterized. The extracted CNCs presented a needle-like morphology, with an average height of 7.27 nm and a length of 221.34 nm, with 65.75% crystallinity, were stable at pH 7–8, and presented thermogravimetric mass loss of 8.0%. Methylcellulose films containing 0.6% w/w of CNC were produced by casting and characterized in terms of thermal, mechanical, and optical properties. Notably, the incorporation of CNCs resulted in significantly more flexible and less rigid films, as evidenced by the higher elongation at break (57.90%) and lower Young’s modulus (0.0015 GPa) compared to neat methylcellulose film. The tensile strength was not affected (p > 0.05). Additionally, the MCNC 0.6% films effectively blocked UV light in the 200–300 nm range without compromising transparency. Altogether, these findings underscore the MCNC 0.6% film as a flexible, biodegradable packaging material suitable for food industry application. Full article

Osteogenesis imperfecta (OI) type XIV is a rare recessive disorder caused by TMEM38B pathogenic variants that disrupt an endoplasmic reticulum protein essential for calcium homeostasis and bone mineralization. This leads to severe bone fragility, early-onset fractures, skeletal deformities, low bone mass, scoliosis, and variable features like blue sclerae or dental abnormalities. We present a case report of a 21-year-old Italian male with a novel homozygous TMEM38B splice variant (c.112 + 1G > T), detailing the clinical presentation, genetic findings, and therapeutic outcomes. The patient exhibited multiple skeletal deformities and showed a moderate response to bisphosphonate therapy (neridronate). In addition, a systematic review of PubMed and Scopus identified 12 relevant studies from an initial set of 82 publications, encompassing data from 56 patients diagnosed with OI type XIV. Unlike classical collagen-related OI, TMEM38B-related OI necessitates genetic screening beyond classical collagen genes (COL1A1 and COL1A2). While bisphosphonates provide some clinical benefit, persistent fractures underscore the need for long-term management and innovative therapies. This case report and systematic review enhance understanding of OI type XIV and underscore the clinical importance of TMEM38B variants in bone fragility disorders. Full article

Background: Sarcopenia and sarcopenic obesity are increasingly recognized in kidney transplant recipients (KTRs), yet their molecular underpinnings remain poorly defined. We sought to synthesize current evidence on biomarker associations with muscle loss and function in the post renal transplant setting. Methods: A comprehensive search of PubMed/MEDLINE and Cochrane databases was conducted according to PRISMA guidelines. Studies evaluating biomarkers related to sarcopenia or sarcopenic obesity in adult and pediatric KTRs were included. Quality assessment was performed with the NHLBI tool. Results: Seven studies were included, encompassing 548 KTRs. Myostatin levels predicted sarcopenia in KTRs (cut-off: 390 pg/mL) and inversely correlated with Metabolic equivalent of Tasks (METs), handgrip strength (HGS), and graft performance. Although adiponectin was negatively correlated with body fat, its high-molecular-weight isoform was linked to lower muscle mass and long-term graft decline. Leptin was associated with sarcopenic obesity and lower estimated Glomerular Filtration Rate (eGFR). Insulin like Growth Factor-1 (IGF-1) independently predicted HGS but not muscle mass. Brain-derived neurotrophic factor (BDNF) levels predicted sarcopenia (cut off: 17.8 ng/mL) and reflected physical activity levels. Visfatin showed no association with sarcopenia but it was positively correlated with eGFR. Lastly, certain polymorphisms of Alpha-actinin-3 (ACTN3) were shown to genetically predispose to post-transplant sarcopenia. Conclusions: These emerging candidate biomarkers provide promising mechanistic insight into post-transplant muscle decline and may ultimately support more personalized risk assessment. Further validation is needed, and functional measures remain the most reliable clinical tools at present. Full article

Background/Objectives: Early-stage breast cancer treatments adversely affect components of health-related fitness (HRF) important for treatment tolerability, recovery, and long-term outcomes. Few studies have examined cancer treatment modality-specific effects on HRF. We examined associations of breast cancer treatment modalities, regimens, and combinations with one-year changes in HRF. Methods: Newly diagnosed early-stage breast cancer patients were recruited between 2012 and 2019 for the Alberta Moving Beyond Breast Cancer (AMBER) cohort study. HRF assessments were completed within 90 days of diagnosis and at one year, including cardiorespiratory fitness, muscle strength and endurance, and body composition. Analysis of covariance was used to test whether HRF changes differed between treatment modalities, regimens, and combinations. All tests were 2-sided. Results: A total of 1350 participants (mean [SD] age, 55.6 [10.7] years) were included. Women who received chemotherapy (n = 797; 59%) experienced statistically significant smaller increases in upper body strength (−1.7 kg, 95% confidence interval [CI]: −3.0 to −0.5), greater declines in lower body endurance (−118.0 kg, 95%CI: −216.6 to −19.3), and greater declines in total lean mass (−0.7 kg, 95%CI: −1.1 to −0.3), bone mineral density (−0.01 g/cm², 95%CI: −0.02 to 0.00), and bone mineral content (0.04 kg, 95%CI: −0.06 to −0.02). Other treatment modalities were modestly and inconsistently associated with HRF changes. Treatment combinations that included chemotherapy had the most negative impact on cardiorespiratory fitness and body composition. Conclusions: Chemotherapy—either alone or in combination with other treatments—had the largest and broadest negative impact on HRF recovery in early-stage breast cancer at one-year follow-up. Full article

Swellable microparticles are a promising strategy for pulmonary drug delivery. They provide good aerosol performance in the dry state and enlarge after deposition in the lungs. In this study, we aimed to develop and characterize spray-dried microparticles composed of carboxymethyl chitosan (CMC), L-leucine, and suramin, a hydrophilic polyanionic drug. Microparticles were obtained by co-spray drying (Co-SD) formulations with increasing leucine content (0–10% w/w) and evaluated for morphology, thermal behavior, crystallinity, swelling, aerodynamic deposition using a Next Generation Impactor (NGI), and cytocompatibility in pulmonary epithelial cells. The 10% leucine formulation produced the highest fine particle fraction (35.2 ± 1.1%) and the lowest mass median aerodynamic diameter (1.0 ± 0.4 µm). These values indicate efficient in vitro deep lung deposition. XRPD and DSC showed that the Co-SD formulations were predominantly amorphous. Hydration studies revealed rapid water uptake and a clear increase in particle size, leading to the formation of swollen microgels. Cell viability assays demonstrated >85% viability up to 100 µM suramin, suggesting that CMC–leucine microgels enable efficient pulmonary delivery of hydrophilic drugs by combining respirable dry-state properties with in situ swelling and reducing immunological clearance. Future in vivo studies will be needed to assess long-term stability, macrophage interaction, and the translational potential of this delivery system. Full article

Hair mineral analysis (HMA) has emerged as a promising non-invasive method for assessing long-term exposure to trace elements and metals, potentially complementing traditional biochemical and clinical markers of cardiovascular risk. This review synthesizes current evidence on the relationships between hair elemental profiles and cardiovascular disease (CVD), with an emphasis on toxic metals (As, Hg, Pb, Cd, Ni, Al) and essential micronutrients (Mg, Mn, Zn, Cu, Fe, Cr, Li). The reviewed studies consistently show that patients with CVD exhibit elevated levels of toxic elements and reduced concentrations of protective ones, reflecting oxidative stress, inflammation, and endothelial dysfunction as mechanistic links. Methodologically, the review highlights inductively coupled plasma mass spectrometry (ICP-MS) with collision/reaction cell technology and microwave digestion as gold-standard analytical approaches, while underscoring the urgent need for harmonized protocols, validated washing procedures, and certified reference materials. The interpretation of HMA requires consideration of temporal dynamics, external contamination, and regional variability. Although current evidence supports the research utility of HMA, its clinical integration remains limited by the absence of reference ranges and prospective validation. HMA may hold future value in environmental risk stratification and primary prevention in exposed populations, but further standardization and large-scale longitudinal studies are necessary to define its diagnostic and prognostic relevance in cardiovascular medicine. Full article

This study compared the muscle quality of largemouth bass (Micropterus salmoides) reared in a simplified indoor factory-scale recirculating aquaculture system (F-RAS) with those reared in a traditional pond (TP). Juveniles from the same cohort (with a mean initial body mass of approximately 16 g) were stocked into the two systems and reared for ten months. The F-RAS is a high-density indoor system utilising octagonal concrete tanks with an effective water volume of 100 m³ and a stocking density of 130 fish m⁻³. The TP is a low-density system, where fish are reared in earthen ponds with a total area of 4000 m² at a density of 1.7 fish m⁻³. At the end of the experiment, 20 fish per group were randomly sampled for morphological analysis, while subsets of 6 fish per group were used for texture analysis, 3 fish per group for water-holding capacity, 3 fish per group for proximate composition, and 9 fish per group (pooled into 3 biological replicates) for amino acid, fatty acid and volatile compound analyses. The results showed that the F-RAS group exhibited superior texture, with significantly higher chewiness, springiness and muscle fibre density. Nutritionally, the F-RAS group had significantly greater crude protein, EPA, DHA and total n-3 fatty acid contents. Although glutamate and leucine levels were lower in the F-RAS group, cysteine and histidine levels were higher. Analysis of volatile compounds indicated improved flavour in the F-RAS group, with a marked reduction in off-flavour compounds such as 1-octen-3-ol and hexanal. Overall, largemouth bass produced in F-RAS showed better muscle quality than those from the TP in terms of texture, nutritional value and flavour. This study provides a reference for future research on the regulation of muscle quality in largemouth bass using a simplified F-RAS. Full article

Background/Objectives: Pregnancy represents a unique physiological state marked by extensive metabolic adaptations, particularly in lipid pathways essential for maternal adjustments, fetal development, and postpartum recovery. This study aimed to explore these changes through untargeted lipidomic profiling. Methods: This observational, comparative, non-interventional clinical study included 107 women, of which 65 were in the third trimester of pregnancy (mean age 27.9 ± 5 years) and 42 were in the early postpartum period (≤7 days, mean age 28.9 ± 5.9 years). Inclusion criteria were singleton, term pregnancies (37–41 weeks) with neonates weighing > 2500 g and no associated pregnancy-related pathologies; exclusion criteria included multiple gestation, use of lipid-altering medications, maternal age > 40 years, or diagnosed pregnancy complications. Plasma samples were analyzed using High-Performance Liquid Chromatography–Quadrupole Time-Of-Flight–Electrospray Ionization (positive mode)–Mass Spectrometry, data were processed with MetaboAnalyst 6.0 using multivariate and univariate analyses (Partial Least Squares–Discriminant Analysis, Volcano Plot, Random Forest, Receiver Operating Characteristic analysis), with statistical significance set at p < 0.05. Results: Multivariate analysis demonstrated a clear separation between groups with high predictive accuracy as reflected by strong classification metrics (Accuracy = 0.90, R² = 0.75, Q² = 0.68). Several discriminative lipids were consistently identified across statistical models, including 2-Methoxyestrone (AUC = 0.861), Eicosanedioic acid (AUC = 0.854), and Pregnenolone sulfate (AUC = 0.843). These biomarkers were further categorized into five major lipid classes: steroid hormones, long-chain fatty acids, lysophospholipids, ceramides/sphingolipids, and glycerolipids. Conclusions: Untargeted lipidomic profiling revealed distinct metabolic signatures that differentiate late pregnancy from early post-partum states. The identification of robust lipid biomarkers with high discriminative performance highlights their potential utility in maternal health monitoring, obstetric risk assessment, and postpartum recovery surveillance. Full article

Obesity is a significant global health challenge, with prevalence rising steadily worldwide. Effective management often involves bariatric surgery, such as sleeve gastrectomy (SG), which has proven effective in reducing weight and improving comorbid conditions. This study aimed to evaluate nutritional status and protein source awareness and its impact on muscle mass among patients post SG surgery in Saudi Arabia. A cross-sectional study was conducted among 98 adults (aged 18–51 years) who underwent SG at the Department of Metabolic and Bariatric Surgery (Badana Clinic)/Mouwasat Hospital in Dammam from December 2023 to February 2024. Data were collected using an electronic questionnaire that covered demographics, dietary habits, physical activity, protein knowledge (from food and supplements), and a 24 h dietary recall. Body composition, including muscle mass, was assessed using the InBody270 Body Composition Analyzer. The results revealed that participants exhibited a high level of awareness regarding the importance of protein sources. However, muscle mass decreased by an average of 4.11 kg after surgery. This decline was attributed to insufficient protein intake, which ranged between 30 and 60 g per day, below recommended levels. Taste aversion and dietary limitations post-surgery contributed to this inadequate protein consumption. In conclusion, while participants understood the significance of protein for muscle maintenance, practical challenges in achieving adequate protein sources led to muscle mass loss. These findings underscore the importance of tailored nutritional strategies and education to optimize recovery and long-term health outcomes for SG surgery patients. Full article