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Keywords = knowledge/attitudes transmission

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16 pages, 1767 KB  
Article
Determinants of HIV/AIDS Knowledge, Attitudes, and Practices Among High School Students in Burayu Town, Ethiopia
by Alemayehu Godana Birhanu, Yalemsew Tefera and Fitsum Tigu
Venereology 2026, 5(1), 2; https://doi.org/10.3390/venereology5010002 - 5 Jan 2026
Viewed by 62
Abstract
Background: HIV is one of the deadliest viruses in the world and the leading cause of mortality and morbidity in the Sub-Saharan African countries, including Ethiopia. Millions of new HIV infections are reported every year among the population in the 15–24 age group. [...] Read more.
Background: HIV is one of the deadliest viruses in the world and the leading cause of mortality and morbidity in the Sub-Saharan African countries, including Ethiopia. Millions of new HIV infections are reported every year among the population in the 15–24 age group. Particularly, adolescents are vulnerable to HIV infection due to a lack of adequate information and engagement in risky sexual practices. Thus, the assessment of knowledge, attitudes, and practices (KAPs) is one of the cornerstones in the fight against HIV/AIDS for designing appropriate prevention strategies. This study aimed to assess the KAPs regarding HIV/AIDS among high school students in Burayu town, Ethiopia. Methods: A cross-sectional study design was conducted on students from three higher secondary schools in Burayu town from February to June 2022. A total of 394 students were selected using a stratified proportional sampling technique. Data were collected using pre-tested and self-administered questionnaires and analyzed by SPSS version 26. Descriptive statistics were conducted, and logistic regression was used to determine the associations between variables. A p-value ≤ 0.05 was considered significant in all cases. Results: Out of the 394 students, the majority of respondents (70%) had sufficient knowledge about HIV/AIDS transmission and prevention, while only 61% demonstrated a positive attitude towards people living with HIV/AIDS (PLWHA). In bivariate analysis, a higher level of knowledge was significantly associated with a positive attitude towards PLWHA at OR 23.4, 95% CI, 2.9–185.5, p = 0.003, and not having had sexual practice at OR 4.1, 95% CI, 1.2–13.9, p = 0.025. In multivariate analysis, sexual practice was significantly (p ≤ 0.05) associated with the level of knowledge, age, gender, and places of residence at AORs values of 5.5, 9.7, 3.4, and 5.5, respectively. Conclusions: This study suggests that students with high levels of knowledge had a positive attitude towards PLWHA and less sexual practice compared to students with low levels of knowledge. Furthermore, students’ sexual practice was significantly associated with knowledge level, age, gender, and place of residence. This underscores the urgent need for targeted education to empower vulnerable groups, foster positive attitudes and practive, and curb HIV/AIDS transmission for improved health outcomes. Full article
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21 pages, 542 KB  
Article
Evaluating Knowledge, Attitudes and Practices Related to Water, Sanitation and Hygiene (WASH): A Case Study of Durban High Schools in South Africa
by Magareth Thulisile Ngcongo and Memory Tekere
Int. J. Environ. Res. Public Health 2026, 23(1), 61; https://doi.org/10.3390/ijerph23010061 - 31 Dec 2025
Viewed by 276
Abstract
Inadequate hygiene knowledge and poor sanitation practices remain key challenges to safe learning environments in South Africa, with 462 million learners attending schools without basic handwashing facilities and many schools failing to meet sanitation standards. Although national policies and infrastructure investments have improved [...] Read more.
Inadequate hygiene knowledge and poor sanitation practices remain key challenges to safe learning environments in South Africa, with 462 million learners attending schools without basic handwashing facilities and many schools failing to meet sanitation standards. Although national policies and infrastructure investments have improved water, sanitation, and hygiene (WASH) services in some schools, access and behaviours remain uneven across socio-economic contexts. This study evaluated knowledge, attitudes, and practices (KAP) related to WASH among 1200 learners from 40 high schools in Durban using a cross-sectional design and interviewer-administered questionnaires. Data were analysed using descriptive statistics, Pearson correlations, ANOVA, and multiple regression. The study addressed the research question: To what extent do learners’ knowledge and attitudes predict hygiene practices across socio-economic contexts? It was hypothesised that higher knowledge and more positive attitudes would significantly predict improved hygiene practices. Results showed that while 74.6% reported handwashing after toilet use, only 39.3% consistently used soap. Knowledge of disease transmission through unsafe water was 35.4%, although overall attitudes were positive. Learners from higher-quintile schools had significantly better KAP scores than those from lower quintiles (p < 0.001). Both knowledge (β = 0.232, p < 0.001) and attitudes (β = 0.266, p < 0.001) were significant predictors of learners’ hygiene practices. Significant group differences were also observed by gender (t = 18.032, p = 0.001) and district (t = −3.895, p = 0.001). These findings highlight persistent WASH gaps and inequities across schools, underscoring the need for integrated interventions that strengthen both hygiene education and school infrastructure to achieve Sustainable Development Goal 6. Full article
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29 pages, 714 KB  
Article
Designing a Teaching–Learning Sequence to Cultivate Plant Awareness Through Transformative Learning
by Alexandros Amprazis and Penelope Papadopoulou
Educ. Sci. 2026, 16(1), 46; https://doi.org/10.3390/educsci16010046 - 30 Dec 2025
Viewed by 572
Abstract
Plant awareness, which refers to the ability to notice, value, and understand the importance of plants, has emerged as a significant research field, particularly considering the growing concerns about sustainability and biodiversity loss. Acknowledging the crucial role of plants in sustaining life on [...] Read more.
Plant awareness, which refers to the ability to notice, value, and understand the importance of plants, has emerged as a significant research field, particularly considering the growing concerns about sustainability and biodiversity loss. Acknowledging the crucial role of plants in sustaining life on Earth and human well-being, several studies highlight the need for educational interventions that can meaningfully enhance plant awareness. In this context, the present study aims to design, implement, and evaluate a Teaching–Learning Sequence (TLS) with university students in a Pedagogical Department. The TLS was grounded in the principles of transformative learning, an educational approach focused not merely on the transmission of knowledge but on fostering deep, personal shifts in learners’ perceptions and attitudes. To assess its impact, the Plant Awareness Disparity Index (PAD-I) was used before and after the implementation, supported by systematic observations and focus group discussions. Results indicate that the TLS effectively enhanced specific dimensions of plant awareness, particularly relative interest between plants and animals and attitudes toward plants. These findings position transformative learning as a promising pedagogical framework for promoting plant awareness in higher education and pave the way for its future application in earlier educational levels. Full article
(This article belongs to the Special Issue Teaching and Learning Sequences: Design and Effect)
15 pages, 538 KB  
Article
Nursing Students’ Knowledge, Attitudes, and Practices Toward Monkeypox Virus: A Cross-Sectional Survey at the University of Palermo, 2022
by Barbara Ravazzolo, Francesco Leonforte, Letizia Cascio, Clara Ferrara, Federico Li Causi, Francesco Armetta, Maria Lampasona, Rinaldo Stefano Miceli, Carlo Fantini, Klara Komici and Alberto Firenze
Zoonotic Dis. 2026, 6(1), 1; https://doi.org/10.3390/zoonoticdis6010001 - 25 Dec 2025
Viewed by 166
Abstract
Background: Human monkeypox is a zoonotic disease caused by the monkeypox virus (MPXV), endemic in Central and West Africa. A significant 2022 outbreak affected 104 countries, driven by increased susceptibility due to the cessation of smallpox vaccination, global travel, and interactions with infected [...] Read more.
Background: Human monkeypox is a zoonotic disease caused by the monkeypox virus (MPXV), endemic in Central and West Africa. A significant 2022 outbreak affected 104 countries, driven by increased susceptibility due to the cessation of smallpox vaccination, global travel, and interactions with infected animals. Strengthening surveillance, public health measures, and raising awareness are essential for early diagnosis, vaccination acceptance, and preventing future outbreaks. Methods: The survey was distributed to 645 nursing students of the University of Palermo between July 2022 and August 2022, and we estimated the knowledge, attitudes and behaviors through a KAP Survey. Descriptive statistics were used to summarize the data, while chi-squared, Fisher’s exact, and Student’s t-tests were employed to analyze differences between groups, with statistical significance set at p < 0.05. At least 80% of the students surveyed had already taken the microbiology exam, as microbiology is studied in the first year of the Bachelor of Science in Nursing program at the University of Palermo. Results: The survey showed a predominantly young, female nursing student population with limited knowledge of monkeypox, as only 3.88% demonstrated adequate understanding. Despite this, attitudes toward prevention were positive, with 82.64% scoring adequately and 41.09% expressing strong willingness to get vaccinated. Knowledge improved with academic progression (p = 0.005), while attitudes and behaviors remained consistent. These findings imply a critical gap in education; the limited knowledge, especially regarding transmission and severity, may lead to an underestimation of the risks associated with global infectious diseases. This underscores the need to better prepare future healthcare professionals for public health emergencies Conclusions: The study found that nursing students had limited knowledge of monkeypox, especially regarding epidemiology and disease severity. Despite positive attitudes toward vaccination, some behaviors reflected an underestimation of global infection risks. Knowledge improved with academic progression, underscoring the importance of education. Targeted educational programs are needed to enhance awareness and preparedness for future outbreaks. Full article
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20 pages, 1468 KB  
Article
Knowledge, Attitudes, and Biosecurity Practices Regarding African Swine Fever Among Small-Scale Pig Farmers in the Lao People’s Democratic Republic and Cambodia
by Véronique Renault, Ariane Masson, Paeng Xaphokame, Outhen Phommasack, Borin Sear, Samnang Ven and Claude Saegerman
Viruses 2026, 18(1), 34; https://doi.org/10.3390/v18010034 - 24 Dec 2025
Viewed by 391
Abstract
African swine fever (ASF) is a transboundary viral disease that has heavily impacted Southeast Asia since its introduction in 2019. Smallholder pig production systems in Cambodia and the Lao People’s Democratic Republic (the Lao PDR), characterized by low biosecurity, free-ranging practices, and limited [...] Read more.
African swine fever (ASF) is a transboundary viral disease that has heavily impacted Southeast Asia since its introduction in 2019. Smallholder pig production systems in Cambodia and the Lao People’s Democratic Republic (the Lao PDR), characterized by low biosecurity, free-ranging practices, and limited veterinary oversight, remain particularly vulnerable. To assess farmers’ awareness and practices regarding ASF, a knowledge, attitudes, and practices (KAP) survey was implemented between March and September 2023 by Agronomes et Vétérinaires Sans Frontières within the framework of the Biosecurity in Pig Farming (BIG) project. A total of 471 pig farmers, including 56% women, were interviewed across eight provinces using a standardized questionnaire (188 in Cambodia and 283 in the Lao PDR). Results showed that ASF awareness was generally high (92% in Cambodia, 66% in the Lao PDR), yet 15% of Cambodian and 30% of Lao respondents expressed doubts about the presence of ASF in their country. While recognition of ASF symptoms was moderate and positively correlated with farmers’ perceived capacity to identify the disease, knowledge of transmission pathways was low and often misaligned with perceptions. Airborne transmission was frequently cited as a risk, and the risks related to visitors and fomites were underestimated by more than 50% of the farmers. Implementation of biosecurity measures (BSM) was limited, with mean scores of 43% in Cambodia and 27% in the Lao PDR. Risky practices such as swill feeding, free-ranging, sharing of boars, traders, and inadequate carcass disposal remained widespread. Statistical analysis identified education level, herd size, knowledge, perceived risks, and perceived benefits of BSM as the main determinants of biosecurity implementation. Farmers with larger herds or stronger commercial orientation demonstrated higher biosecurity adoption, while misconceptions and knowledge gaps remained frequent among smallholder farmers. Strengthening awareness, promoting low-cost and feasible biosecurity practices, and integrating farmer-centred approaches are essential for reducing ASF transmission risks and improving the resilience of smallholder pig production systems in the region. Full article
(This article belongs to the Special Issue New Findings in Animal Biosecurity Related to Viral Diseases)
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16 pages, 664 KB  
Review
Thirdhand Smoke and Neonatal/Pediatric Health: A Scoping Review on Nursing Implications
by Valentina Vanzi, Marzia Lommi, Alessandro Stievano, Gennaro Rocco, Maurizio Zega and Gabriele Caggianelli
Healthcare 2025, 13(24), 3289; https://doi.org/10.3390/healthcare13243289 - 15 Dec 2025
Viewed by 353
Abstract
Background/Objectives: Thirdhand smoke (THS), residual tobacco pollutants persisting on surfaces, dust, and fabrics, poses specific risks to infants and children, yet its implications for nursing remain underexplored. This scoping review mapped existing evidence on THS in neonatal and pediatric contexts and synthesized [...] Read more.
Background/Objectives: Thirdhand smoke (THS), residual tobacco pollutants persisting on surfaces, dust, and fabrics, poses specific risks to infants and children, yet its implications for nursing remain underexplored. This scoping review mapped existing evidence on THS in neonatal and pediatric contexts and synthesized nursing implications, focusing on nurses’ knowledge, unintentional environmental contamination, and educational roles. Methods: Following JBI methodology and reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, a three-step search was performed across MEDLINE, CINAHL, Scopus, Web of Science, Cochrane Library, Google Scholar, and OpenGrey. Studies were included if they addressed (1) nurses’ knowledge, beliefs, and attitudes toward THS-related risks in infants and children; (2) nurses’ contribution to unintentional environmental THS contamination; or (3) nurse-led educational or preventive interventions targeting parents or communities. Results: Among 563 records, 8 met inclusion criteria. Four investigated nurses’ awareness and perceptions, revealing limited understanding of THS despite recognition of its harmfulness. One study examined contamination, detecting nicotine residues on nurses’ fingers, suggesting possible in-hospital transmission. No nurse-led interventions specifically targeting THS were found, though broader smoke-exposure education programs showed benefits when supported by nursing staff. Conclusions: Evidence is scarce but underscores significant gaps in nurses’ knowledge, clinical guidance, and educational initiatives concerning THS. Strengthening nursing education and research is essential to mitigate THS exposure in neonatal and pediatric settings and enhance nurses’ preventive and advocacy roles. Full article
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14 pages, 269 KB  
Article
Study of the Knowledge, Attitudes and Practices of Students from Medical Majors Regarding HPV Infections and HPV Vaccines
by Meri Hristamyan, Vanya Rangelova, Theodor Lolovski, Meysam Homadi and Ani Kevorkyan
Pathogens 2025, 14(12), 1270; https://doi.org/10.3390/pathogens14121270 - 11 Dec 2025
Viewed by 390
Abstract
Background: Human papillomavirus (HPV) is a highly prevalent sexually transmitted infection that can lead to cervical and other anogenital and oropharyngeal cancers. Despite available vaccines, vaccination coverage remains low in Bulgaria. This study aimes to assess the knowledge, attitudes, and practices of Medical [...] Read more.
Background: Human papillomavirus (HPV) is a highly prevalent sexually transmitted infection that can lead to cervical and other anogenital and oropharyngeal cancers. Despite available vaccines, vaccination coverage remains low in Bulgaria. This study aimes to assess the knowledge, attitudes, and practices of Medical University students in HPV prevention. Materials: A cross-sectional anonymous survey was conducted at the Medical University-Plovdiv, Bulgaria. Results: A total of 1485 students, primarily women (60.1%) with a median age of 22.78 years, participated. Four hundred fifty-two (30.4%) reported having received the HPV vaccine. Of the unvaccinated, 800 (77.8%) expressed willingness to receive the vaccine. Vaccinated respondents were more likely to report having had five or more sexual partners (37.1%) compared to unvaccinated respondents (21.1%) (χ2 = 77.136, p < 0.001). Approximately one-third (36.4%) mistakenly believe condoms provide complete protection and that antibiotics effectively treat HPV. Students who opposed the assertion that vaccinating minors suggests early sexual activity is permissible were 1.89 times more likely to be vaccinated. Conclusions: Medical University students possess insufficient understanding of HPV transmission, health outcomes, and prevention. Their attitudes and practices require improvement. Enhancing the curriculum with comprehensive HPV information will better equip future healthcare providers and improve public health outcomes. Full article
17 pages, 255 KB  
Article
Early Childhood Oral Health: Insights into Knowledge, Preventive Practices, and Risk Awareness from a Croatian Cross-Sectional Study
by Marija Matijević, Marija Badrov, Lidia Gavić and Antonija Tadin
Pediatr. Rep. 2025, 17(6), 130; https://doi.org/10.3390/pediatric17060130 - 3 Dec 2025
Viewed by 396
Abstract
Aim: Early childhood caries (ECC) is a widespread and multifactorial oral disease that affects children globally. Parents’ knowledge, attitudes, and behaviors are crucial in preventing ECC and supporting oral health. This study evaluated Croatian parents’ understanding of children’s oral health, their awareness of [...] Read more.
Aim: Early childhood caries (ECC) is a widespread and multifactorial oral disease that affects children globally. Parents’ knowledge, attitudes, and behaviors are crucial in preventing ECC and supporting oral health. This study evaluated Croatian parents’ understanding of children’s oral health, their awareness of ECC risk factors, and their oral hygiene practices. Materials and methods: A cross-sectional study was conducted using an anonymous and voluntary online questionnaire from October to December 2024 among 948 parents of children aged 1–7 years across Croatia. The study assessed parents’ knowledge of oral health, their understanding of the relationship between risk factors and early childhood caries, habits related to oral hygiene care, children’s experiences with oral health problems, parents’ self-assessment of their knowledge, as well as both their own and their children’s general and oral health and hygiene practices. Data were analyzed using descriptive statistics, Chi-square test, Mann–Whitney U test, and Kruskal–Wallis test. Results: Overall parental knowledge was moderate, with significantly higher scores among older parents, those with university education, healthcare workers, and families with higher incomes (p < 0.05). Parents demonstrated good awareness of the importance of supervising tooth brushing until age seven (93.8%) and fluoride use (81.8%); yet gaps persisted regarding bacterial transmission, tooth eruption, and early orthodontic evaluation. Preventive dental visits were frequently delayed, and only 25.0% of parents reported using interdental cleaning aids. Caries was the most common oral health issue among children (22.3%). Conclusions: Despite moderate awareness and some adherence to preventive measures, significant knowledge and practice gaps remain among Croatian parents. Targeted educational interventions and nationwide preventive strategies are necessary to strengthen oral health literacy and reduce ECC prevalence. Full article
12 pages, 809 KB  
Article
Public Awareness of Rabies and Post-Bite Practices in Makkah Region of Saudi Arabia: Cross-Sectional Study
by Nahla H. Hariri, Khalid S. Alrougi, Abdullah A. Almogbil, Mona H. Kassar, Reman G. Alharbi, Abdullah O. Krenshi, Jory M. Altayyar, Abdullah S. Alibrahim, Maher N. Alandiyjany, Fozya B. Bashal, Nizar S. Bawahab, Saleh A. K. Saleh and Heba M. Adly
Trop. Med. Infect. Dis. 2025, 10(12), 337; https://doi.org/10.3390/tropicalmed10120337 - 29 Nov 2025
Viewed by 957
Abstract
Background: Rabies is a fatal yet preventable zoonosis. In Saudi Arabia, uneven surveillance and limited public awareness may delay post-exposure prophylaxis (PEP). In Makkah, where residents regularly encounter free-roaming dogs, knowledge gaps could elevate exposure risks. Objectives: This study aims to assess public [...] Read more.
Background: Rabies is a fatal yet preventable zoonosis. In Saudi Arabia, uneven surveillance and limited public awareness may delay post-exposure prophylaxis (PEP). In Makkah, where residents regularly encounter free-roaming dogs, knowledge gaps could elevate exposure risks. Objectives: This study aims to assess public knowledge, attitudes, and post-bite practices regarding rabies, including wound washing and access to PEP among adult residents of the Makkah Region, and to examine associations with pet dog ownership. Methods: A cross-sectional survey was conducted in the Makkah Region (March–June 2025). An online validated bilingual questionnaire targeted residents ≥ 18 years via social media. Descriptive statistics, chi-square tests, 95% confidence intervals, and binomial logistic regression were applied in IBM SPSS v26; p < 0.05 was significant. Results: Of 523 respondents, 91.8% lived in Makkah city, 52.8% were female, and the age distribution was 18–24 years (44.2%), 25–34 years (35.6%), 35–44 years (12.0%), and ≥45 years (8.2%). Pet dog ownership was rare (1.9%), yet 39.4% reported stray dogs in their communities. Overall, 60.6% knew what rabies is and 63.7% knew it is vaccine-preventable, but 52.2% wrongly believed that transmission occurs only via dog bites. Hospitals (79.7%) and health centers (79.2%) were the most cited vaccination sites; social media was the dominant information source (74.6%). No significant association was found between pet ownership and rabies awareness (all p > 0.05). In multivariable regression (n = 509), adequate rabies knowledge increased the odds of an appropriate intended response (AOR 1.85, 95% CI: 1.27–2.68). Participants aged 30–40 years and those >50 years had significantly lower odds (AOR 0.45, 95% CI: 0.24–0.85 and AOR 0.23, 95% CI: 0.09–0.56, respectively). Conclusions: Despite moderate awareness, critical misconceptions and inconsistent first aid intentions persist. Priority actions include clear, locally adapted education on immediate wound washing and prompt PEP, standardized bite management pathways across facilities, reliable access to vaccines and immunoglobulin, and targeted social media micro-campaigns. By identifying public misconceptions, knowledge gaps, and preferred communication channels, this study provides baseline evidence to guide community awareness programs, intersectoral collaboration, and One Health-based surveillance essential for Saudi Arabia’s progress toward the global “Zero rabies by 2030” goal. Full article
(This article belongs to the Special Issue Rabies—Global Challenges, Societal Perspectives, and Case Studies)
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13 pages, 225 KB  
Article
Knowledge, Attitude and Practices of Primary Care Physicians Regarding Infection Control of Tuberculosis in Primary Health Care Centers, Riyadh, Saudi Arabia
by Yasser Alhazzani, Abdulaziz Nasser Alahmari, Bandar K. AlRabiah, Khalid F. Alsadhan, Abdulaziz Yahya Sahhari and Fahad Alrabieah
Infect. Dis. Rep. 2025, 17(5), 134; https://doi.org/10.3390/idr17050134 - 20 Oct 2025
Viewed by 505
Abstract
Background: Tuberculosis (TB) remains a public health concern in Saudi Arabia, where primary care physicians play a crucial role in early detection and infection control. This study assessed physicians’ knowledge, attitudes, and practices (KAP) regarding TB infection control in Riyadh. Methods: A cross-sectional [...] Read more.
Background: Tuberculosis (TB) remains a public health concern in Saudi Arabia, where primary care physicians play a crucial role in early detection and infection control. This study assessed physicians’ knowledge, attitudes, and practices (KAP) regarding TB infection control in Riyadh. Methods: A cross-sectional survey was conducted among 205 physicians in primary healthcare centers using a validated electronic questionnaire. Knowledge scores were classified as good (≥8/14 correct) or poor (<8). Descriptive statistics and chi-square/t-tests were applied. Results: The mean knowledge score was 8.5 (SD = 2.1); 57.1% of physicians demonstrated good knowledge. Knowledge was significantly associated with specialization (p = 0.049), position (p = 0.031), and monthly patient load (p = 0.031). While 92.7% correctly identified airborne transmission, only 30.7% knew when a TB patient becomes noninfectious. Most participants (80%) had not received TB-related training in the past year. Conclusions: Primary care physicians in Riyadh show moderate knowledge and positive attitudes, but important gaps remain in diagnostic clarity and infection control timelines. Strengthening continuous medical education and integrating TB-specific modules into the Saudi national TB control program are essential to standardize practices and improve patient outcomes. Full article
(This article belongs to the Section Viral Infections)
10 pages, 8316 KB  
Article
Public Health Communication on Emerging Vector-Borne Disease Risk in Gaya and Shahpori Islands
by Muhammad Belal Hossain, Sadia Choudhury Shimmi, M Tanveer Hossain Parash and Phoebe Tran
Parasitologia 2025, 5(3), 45; https://doi.org/10.3390/parasitologia5030045 - 1 Sep 2025
Viewed by 1310
Abstract
Vector-borne diseases (VBDs) continue to pose a persistent global health challenge, disproportionately impacting low- and middle-income countries where surveillance and healthcare infrastructure are constrained. Within these countries, residents of remote island communities are particularly vulnerable to emerging VBD threats; however, they remain critically [...] Read more.
Vector-borne diseases (VBDs) continue to pose a persistent global health challenge, disproportionately impacting low- and middle-income countries where surveillance and healthcare infrastructure are constrained. Within these countries, residents of remote island communities are particularly vulnerable to emerging VBD threats; however, they remain critically understudied. We conducted a community-based cross-sectional survey of 300 residents from Shahpori Island, Bangladesh, and Gaya Island, Malaysia, to assess knowledge, attitudes, and practices (KAP) related to VBDs. Using a structured questionnaire and mixed-effects Poisson regression modeling, we identified socio-demographic predictors of VBD-related knowledge and compared KAP outcomes across the two islands. Significant disparities were observed between the two settings: the Gaya Island residents demonstrated a higher level of awareness regarding VBDs and preventive practices compared to the Shahpori residents. Education was a strong predictor of VBD knowledge, with individuals possessing secondary education or above exhibiting significantly better knowledge (β = 0.2024, p = 0.0003). Marital status was also a significant factor, with unmarried respondents showing lower levels of knowledge (β = –0.1657, p = 0.0372). Age was positively correlated with VBD knowledge (β = 0.0051, p = 0.0119), indicating a gradual increase with age, while income, gender, occupation, and household size were not significantly associated. Despite basic awareness of VBD symptoms and transmission, detailed understanding of mosquito ecology, disease symptoms, and breeding prevention strategies was remarkably poor, especially among the Shahpori residents. Our findings highlight critical gaps in VBD-related knowledge and prevention behaviors shaped by socio-economic and educational disparities. Community-focused public health strategies including educational campaigns, establishment of health infrastructure, access to trained healthcare providers, and integrated vector control interventions are urgently needed to enhance resilience against emerging VBD threats such as drug-resistant malaria in remote island populations. Full article
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19 pages, 1910 KB  
Review
Hansen’s Disease in Ecuador: Current Status, Knowledge Gaps, and Research Priorities: A Literature Review
by Manuel Calvopiña, Juan S. Izquierdo-Condoy, Esteban Ortiz-Prado, Jorge Vasconez-Gonzalez, Lorena Vaca and Elías Guamán
Pathogens 2025, 14(8), 832; https://doi.org/10.3390/pathogens14080832 - 21 Aug 2025
Viewed by 2866
Abstract
Background: Hansen’s disease or leprosy is one of the 21 neglected tropical diseases (NTDs). In Ecuador, leprosy is considered eliminated as a public health problem; however, new cases are reported annually. Additionally, Mycobacterium leprae infection was detected in nine-banded armadillos across the country, [...] Read more.
Background: Hansen’s disease or leprosy is one of the 21 neglected tropical diseases (NTDs). In Ecuador, leprosy is considered eliminated as a public health problem; however, new cases are reported annually. Additionally, Mycobacterium leprae infection was detected in nine-banded armadillos across the country, suggesting a potential zoonotic reservoir. This literature review aims to provide an updated overview of the epidemiological situation of leprosy in Ecuador, identify knowledge gaps, and outline research priorities to support the development of a comprehensive national strategy for achieving zero autochthonous cases. Methods: This article analyses the current situation of leprosy in Ecuador based on international and national publications. A retrospective literature search using five international, regional, and national publications on leprosy published between 1954 and 2024 (70 years) with no restriction on language or publication date, was performed. Findings: Our review identified 28 publications with the earliest article dating back to 1954. Of these, 14 were published in international journals, 15 (53.6%) were in Spanish. Four nationwide studies documented leprosy cases across Ecuador’s three continental regions (Coast, Andes, and Amazon) with a predominance in the tropical coast. No cases have been reported from the Galápagos Islands. From 1983, Ecuador started multi-drug therapy. Data from the Ministry of Public Health (MoH) system identified 1539 incident cases, showing a significant decline in new cases from 2000 to 2024, with no cases in children. New cases detection rate by 100,000 inhabitants was 0.51 in 2019 according to the World Health Organization (WHO). No study has genotyped the Mycobacterium spp. in human cases, other animal species, or environment. According to the MoH, multibacillary leprosy accounts for 78.95% of diagnosed cases, with confirmation based on Ziehl–Neelsen staining and histopathology. No survey has assessed disabilities, knowledge, attitudes, and practices (KAP) or stigma related to leprosy. Research is needed on transmission routes, Mycobacterium genotyping, genetic susceptibility, and antibiotic resistance. BCG vaccination coverage fell to 75.3% in 2021. Cases are currently diagnosed and treated on an outpatient basis in large hospitals. Conclusions: This comprehensive review highlights persistent gaps in leprosy research and critical information, despite seven decades of documented cases in Ecuador. The disease is still endemic across the country, particularly at subnational level in the subtropics and tropics of the Pacific coast and the Amazon. There is a need for nationwide epidemiological research on reservoirs and the environment applying the One Health concept. Increased laboratory facilities and readily available official data are required to improve our understanding of leprosy in Ecuador. Strengthening community-level efforts is essential for Ecuador to meet the targets of the “WHO’s Towards Zero Leprosy: Strategy 2021–2030.” Full article
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21 pages, 5517 KB  
Article
Artificial Intelligence Disclosure in Cause-Related Marketing: A Persuasion Knowledge Perspective
by Xiaodong Qiu, Ya Wang, Yuruo Zeng and Rong Cong
J. Theor. Appl. Electron. Commer. Res. 2025, 20(3), 193; https://doi.org/10.3390/jtaer20030193 - 2 Aug 2025
Cited by 5 | Viewed by 8117
Abstract
Integrating artificial intelligence (AI) and cause-related marketing has reshaped corporate social responsibility practices while triggering a conflict between technological instrumental rationality and moral value transmission. Building on the Persuasion Knowledge Model (PKM) and AI aversion literature, this research employs two experiments to reveal [...] Read more.
Integrating artificial intelligence (AI) and cause-related marketing has reshaped corporate social responsibility practices while triggering a conflict between technological instrumental rationality and moral value transmission. Building on the Persuasion Knowledge Model (PKM) and AI aversion literature, this research employs two experiments to reveal that AI disclosure exerts a unique inhibitory effect on consumers’ purchase intentions in cause-related marketing contexts compared to non-cause-related marketing scenarios. Further analysis uncovers a chain mediation pathway through consumer skepticism and advertisement attitudes, explaining the psychological mechanism underlying AI disclosure’s impact on purchase intentions. The study also identifies the moderating role of AI aversion within this chain model. The findings provide a new theoretical perspective for integrating AI disclosure, consumer psychological responses, and marketing effectiveness while exposing the “value-instrumentality” conflict inherent in AI applications for cause-related marketing. This research advances the evolution of the PKM in the digital era and offers practical insights for cause-related marketing enterprises to balance AI technology application with optimized disclosure strategies. Full article
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16 pages, 4439 KB  
Article
Baseline Assessment of Taeniasis and Cysticercosis Infections in a High-Priority Region for Taenia solium Control in Colombia
by Carlos Franco-Muñoz, María Camila Jurado Guacaneme, Sonia Dayanni Castillo Ayala, Sofia Duque-Beltrán, Adriana Arévalo, Marcela Pilar Rojas Díaz, Julián Trujillo Trujillo, Luz Elena Borras Reyes, Luis Reinel Vásquez Arteaga, Julio César Giraldo Forero and Mario J. Olivera
Pathogens 2025, 14(8), 755; https://doi.org/10.3390/pathogens14080755 - 31 Jul 2025
Viewed by 4789
Abstract
Coyaima is a town in the department of Tolima, Colombia, that was prioritized in a pilot program under Colombia’s National Plan for the Control of the Taeniasis/Cysticercosis Complex, focusing on this neglected health issue. The project engaged local indigenous communities, promoting education and [...] Read more.
Coyaima is a town in the department of Tolima, Colombia, that was prioritized in a pilot program under Colombia’s National Plan for the Control of the Taeniasis/Cysticercosis Complex, focusing on this neglected health issue. The project engaged local indigenous communities, promoting education and outreach within the One Health framework. The study included 444 randomly selected volunteers, who filled a Knowledge, Attitudes, and Practices (KAP) survey on the taeniasis/cysticercosis complex. The baseline study found no Taenia spp. eggs via microscopy on 383 stool samples examined, and no T. solium DNA was detected on human stool and soil samples by Copro-qPCR. However, seroprevalence was 8.5% for human cysticercosis and 14% for porcine cysticercosis, as detected by in-house ELISA testing for T. solium. Moreover, 57.9% of participants who provided a stool sample were positive for at least one parasite. Following the sampling and characterization activities, local health workers implemented mass treatment with Niclosamide, based on evidence of ongoing transmission, high porcine seroprevalence, poor basic sanitation, and the presence of free-roaming pigs reported in the KAP survey. These findings provide scientific evidence to apply national public health policies for controlling taeniasis/cysticercosis complex in Coyaima. Full article
(This article belongs to the Special Issue Recent Advances in Taeniasis and Cysticercosis)
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Article
Mind the Gap: Knowledge, Attitudes and Practices Regarding Equine Piroplasmosis in Portugal
by Ana Cabete, Elisa Bettencourt, Ludovina Padre and Jacinto Gomes
Parasitologia 2025, 5(3), 38; https://doi.org/10.3390/parasitologia5030038 - 28 Jul 2025
Viewed by 1229
Abstract
Equine piroplasmosis (EP) is a tick-borne disease caused by Theileria equi, Theileria haneyi and Babesia caballi. It affects equids, representing significant health and economic concerns for the equine industry. EP is endemic in Portugal, so developing and implementing preventive strategies is [...] Read more.
Equine piroplasmosis (EP) is a tick-borne disease caused by Theileria equi, Theileria haneyi and Babesia caballi. It affects equids, representing significant health and economic concerns for the equine industry. EP is endemic in Portugal, so developing and implementing preventive strategies is essential. Accessing veterinarians’ knowledge, attitudes and practices (KAP) through a survey is a suitable approach, and no such studies have been conducted in Portugal until now. A KAP survey was applied to 41 Portuguese equine vets, representing mainly the Alentejo region. The average knowledge score went from medium to high, correctly identifying the causative agents, transmission routes and clinical signs. Knowledge gaps mostly concerned the identification of T. haneyi as an agent, transplacental transmission, duration of infection and diagnostic methods. Reported practices were appropriate overall, including enhancing breeders’ awareness of the disease and its prevention. Diagnostic and treatment protocols were generally consistent with current recommendations; however, these protocols are not yet fully standardized. Our findings highlight key areas where increasing expertise is needed and could serve as a foundation for future evidence-based guidelines to improve EP control in Portugal. Full article
(This article belongs to the Special Issue New Insights on Veterinary Parasites)
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