Search Results (30)

Autochthonous Chagas disease remains a health risk for humans in Latin American countries but is rarely found among residents of the United States (US), despite the presence of competent insect vectors and small mammal reservoirs of Trypanosoma cruzi in the lower two-thirds of the US. This report discusses the differences in the rates of autochthonous Chagas disease in Latin America and the US. The key to the differences may lie in the mode (or means) of transmission of parasites to humans. In both Latin America and the US, the so-called vectorial transmission of Chagas disease to humans is the mode of acquisition accepted by most authorities. This mode involves the improbable combination of an infected kissing bug defecating near the mouth or eyes or the site of the bite, followed by the bite victim rubbing infected feces into the wound site or mucous membranes. Outbreaks of Chagas disease due to fecal–oral contamination, known as oral Chagas, have been recorded in Latin America for decades, and at present, oral Chagas is the predominant mode of infection recognized in Brazil. It is perhaps time to consider fecal–oral transmission in its many manifestations as a risk factor for Chagas disease in the US rather than reflexively invoking vectorial transmission. Fecal–oral transmission includes contamination of food and drink by triatomine feces and infection via contaminated fomites and surfaces at home and at worksites, as well as transmission from infected small mammals and other routes discussed in this report. Full article

The kissing bug Rhodnius prolixus is a nocturnal species; however, its temporal mating patterns remain unexplored. Copulation lasts about fifty minutes, during which time the couple remains almost motionless. We hypothesized that R. prolixus copulates within shelters to reduce its vulnerability. To test this, we examined the spatial and temporal patterns of its copulation, as well as the potential endogenous regulation of its circadian rhythm, under three conditions: a light:dark cycle (L/D), constant light (L/L), and constant darkness (D/D). Over ten days, the number and the timing of copulation of pairs in arenas containing a shelter were video-recorded. Under the L/D cycle, the pairs mated mainly during daylight hours. This rhythm persisted under the D/D cycle, with a peak extending from the first half of the subjective day to the first half of the subjective night. No rhythm was observed under the L/L cycle. A greater proportion of mating occurred within the shelters during the L/D and L/L cycles, whereas its spatial distribution was random under the D/D cycle. Our results reveal an endogenously controlled circadian rhythm of mating behavior, with mating activity mainly occurring during daylight hours. Additionally, we showed that the pairs copulated inside the shelters. These results provide new insights into the reproductive behavior of R. prolixus. Full article

Triatoma sordida is a native South American species and the most frequently captured triatomine in artificial environments in Brazil. Although considered a secondary vector of Trypanosoma cruzi, it is typically associated with low infection rates. To investigate its role in an endemic area for Chagas disease in northern Minas Gerais, Brazil, we employed a multidimensional approach that combined triatomine capture data with quantitative and qualitative analyses of T. cruzi. A total of 1861 T. sordida specimens were captured, of which 1455 were examined and 210 (14.4%) were found to be infected with T. cruzi. The most prevalent discrete typing unit (DTU) was TcI (80%), followed by TcII (8%), TcV (5%), and TcIII (3%). Molecular techniques provided new insights into the ecology of T. sordida, revealing a higher infection rate than previously reported and a parasitic load lower than that observed in other quantified species. Chickens were confirmed as the primary food source, playing an epidemiological role in maintaining infected insects with four T. cruzi DTUs. The observed diversity of T. cruzi DTUs suggests a lack of environmental segregation, likely due to the extensive movement of various host species between wild and domestic habitats, resulting in overlapping transmission cycles. Full article

Morphometry is an effort to describe or measure the morphology of the body, or parts of it. It also provides quantitative data on the interactions of living organisms with their environment, external or internal. As a discipline, morphometrics has undergone significant developments in the last decade, making its implementation more visual and less laborious. Chagas disease vectors, often referred to by the common name of “kissing bugs”, belong to the subfamily Triatominae. Due to their apparent morphological plasticity, they have been the subject of numerous morphometric studies. Most of these have been applied taking into account the particularities of this group of vectors, such as domesticity (synanthropy), food preferences, dispersal ability, insecticide resistance, as well as some taxonomic issues. This brief review over nearly three decades is organized here according to the body organs considered by the authors. Full article

The epidemiology of Chagas disease in humans has markedly changed within the past several decades in the United States of America. This report discusses autochthonous cases of Chagas disease as well as disease in immigrants from Latin American countries. Suggestions for epidemiology research and medical care are discussed given the evolving epidemiology of the disease in the United States of America. Full article

The hemoflagellate parasite Trypanosoma rangeli is transmitted by triatomine kissing bugs and may co-infect humans together with its Chagas disease-causing congener T. cruzi. Using real-time quantitative polymerase chain reaction (RT-qPCR) and antimicrobial assays, we studied (i) the temporal and spatial distribution of T. rangeli in common bed bugs, Cimex lectularius, following oral ingestion and hemocoelic injection of T. rangeli, and (ii) the immune responses of bed bugs induced by T. rangeli infections. Irrespective of infection mode, no live T. rangeli were present in the bed bugs’ hemolymph, salivary glands, or feces. On day 1 following infection, the bed bugs strongly upregulated the antimicrobial peptide CL-defensin. Following hemocoelic injection of T. rangeli, live parasites were absent in any bed bug tissues examined throughout the 10-day study period. The ingestion of T. rangeli-infected blood had no significant effect on bed bug survival. Our findings indicate that bed bugs disable the development of T. rangeli within their body, in stark contrast to triatomine kissing bugs, which allow the development and transmission of T. rangeli. Our findings help unravel the intricate relationships between bed bugs and trypanosomes, and they contribute to our understanding of vector biology. Full article

The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, also called American trypanosomiasis. This neglected tropical disease affects millions of individuals across the Americas. To complete its life cycle, T. cruzi parasitizes both vertebrate hosts and its vector, commonly known as the ‘kissing bug’. The parasite’s survival and proliferation strategies are driven by the diverse environments it encounters. Despite being described by Carlos Chagas in 1909, significant knowledge gaps persist regarding the parasite’s various life forms and adaptive capabilities in response to environmental cues. In this study, we employed Ultrastructure Expansion Microscopy to explore the intricate journey of T. cruzi within the host cell. Upon entry into the host cell, trypomastigotes undergo folding, resulting in intermediate forms characterized by a rounded cell body, anterior positioning of basal bodies, and a shortened flagellum. The repositioning of basal bodies and the kinetoplast and the shortening of the flagella mark the culmination of intracellular amastigogenesis. Furthermore, we analyzed intracellular trypomastigogenesis, identifying discrete intermediate forms, including leaf-shaped stages and epimastigote-like forms, which suggests a complex differentiation process. Notably, we did not observe any dividing intracellular epimastigotes, indicating that these may be non-replicative forms within the host cell. Our detailed examination of amastigote cell division revealed semi-closed nuclear mitosis, with mitotic spindle formation independent of basal bodies. This study provides new insights into the morphological and cytoskeletal changes during the intracellular stages of T. cruzi, providing a model for understanding the dynamics of intracellular amastigogenesis and trypomastigogenesis. Full article

Female insects belonging to the genera Anopheles, Aedes, Glossina, and Rhodnius account for the majority of global vector-borne disease mortality. In response to mating, these female insects undergo several molecular, physiological, and behavioral changes. Studying the dynamic post-mating molecular responses in these insects that transmit human diseases can lead to the identification of potential targets for the development of novel vector control methods. With the continued advancements in bioinformatics tools, we now have the capability to delve into various physiological processes in these insects. Here, we discuss the availability of multiple datasets describing the reproductive physiology of the common blood-feeding insects at the molecular level. Additionally, we compare the male-derived triggers transferred during mating to females, examining both shared and species-specific factors. These triggers initiate post-mating genetic responses in female vectors, affecting not only their reproductive success but also disease transmission. Full article

Chagas disease, caused by the Trypanosoma cruzi parasitic protozoan, is a neglected tropical disease (NTD) of significant incidence in Latin America. Transmission to humans and other mammals is mainly via the vector insect from the Reduviidae family, popularly known as the kissing bug. There are other transmission means, such as through congenital transmission, blood transfusions, organ transplantations, and the consumption of contaminated food. For more than 50 years, the disease has been treated with benznidazole and nifurtimox, which are only effective during the acute phase of the disease. In addition to their low efficacy in the chronic phase, they cause many adverse effects and are somewhat selective. The use of nanocarriers has received significant attention due to their ability to encapsulate and release therapeutic agents in a controlled manner. Generally, their diameter ranges from 100 to 300 nanometers. The objective of this scoping review was to perform a search of the literature for the use of nanocarriers as an alternative for improving the treatment of Chagas disease and to suggest future research. Bibliographic searches were carried out in the Web of Science and PubMed scientific databases from January 2012 to May 2023, using the “Chagas disease and Trypanosoma cruzi and nanoparticles” keywords, seeking to gather the largest number of articles, which were evaluated using the inclusion and exclusion criteria. After analyzing the papers, the results showed that nanocarriers offer physiological stability and safety for the transport and controlled release of drugs. They can increase solubility and selectivity against the parasite. The in vitro assays showed that the trypanocidal activity of the drug was not impaired after encapsulation. In the in vivo assays, parasitemia reduction and high survival and cure rates in animals were obtained during both phases of the disease using lower doses when compared to the standard treatment. The scoping review showed that nanocarriers are a promising alternative for the treatment of Chagas disease. Full article

The usefulness of blood collection using venipuncture versus kissing bugs or medicinal leeches and the collection of saliva, faeces, hair, urine, and tears for measuring “immunoreactive” C (iC) concentration in Alpine ibexes was verified using commercial enzyme immunoassays. The mean value of serum C was highest in serum collected using venipuncture and lowest in serums collected using kissing bugs. Statistically significant differences were observed between venipuncture and kissing bugs and between leeches and kissing bugs. However, no statistically significant difference was found in C concentrations between samples collected with venipuncture and those collected with leeches. The highest mean value of C concentration was measured in serum (all three methods), followed by that in hair and faeces, and the lowest mean value was found in saliva. Statistically significant differences were found between saliva and faeces samples and between saliva and hair samples. The difference between the concentrations for faeces and hair was not statistically significant. A significant difference in C concentration between males and females was found in saliva. A significant difference in C concentration among different ages was measured in serum obtained using venipuncture in all three groups and in faeces between the groups older than ten years and younger than 10 months. Highly significant differences in C concentrations were also found between hair sampled in summer and hair sampled in autumn. Collecting tear and urine samples is a laborious procedure and is therefore less acceptable for C determination. Due to the small number of samples, statistical values are not given for these two matrices. Full article

Triatominae, the only blood-sucking subfamily in Reduviidae, are the vectors of Chagas disease. The majority of them are distributed in the Americas, while the diversity in China has been underestimated, as only two species have been recorded. Here, we describe two new species from China, Triatoma picta Zhao & Cai sp. nov. and T. atrata Zhao & Cai sp. nov., and provide a redescription of T. sinica Hsiao, 1965, along with remarks on T. rubrofasciata (De Geer, 1773). To facilitate the identification, we include photos, especially of genitalia, as well as a distribution map and a key to Chinese triatomines. We calculated the pairwise genetic distances between 23 Triatoma species, which further supported the validity of these new species. We anticipate that our taxonomic review will be useful for identifying Chinese Triatominae. Full article

The gross anatomy of the male reproductive structures and their associated musculature are described in the blood-gorging vector of Chagas disease, Rhodnius prolixus. The male reproductive system is composed of muscular tissues each performing contractions that aid in the movement of sperm out of the testis into the vas deferens, seminal vesicle and then into the ejaculatory duct, along with proteins and lipids from the transparent and opaque accessory glands. Phalloidin staining shows the various patterns of muscle fiber layers, from thin circular to more complex crisscross patterns, implying subtle differences in the form of the contractions and movement of each of the structures, allowing for waves of contractions or twisting patterns. The transcripts for the receptors for proctolin, myosuppressin (RhoprMS) and for the extended FMRFamides are expressed in the various regions of the reproductive system, and the nerve processes extending over the reproductive structures are positive for FMRFamide-like immunoreactivity, as are neurosecretory cells lying on the nerves. Proctolin and AKDNFIRFamide are strong stimulators for the frequency of the contractions, and RhoprMS can inhibit the proctolin-induced contractions. Taken together, this work implicates these two families of peptides in coordinating the male reproductive structures for the successful transfer of sperm and the associated accessory gland fluid to the female during copulation. Full article

In this study, we evaluated the effect of the climatic season and infection by Trypanosoma cruzi, etiological agent of Chagas disease, on the molting capacity of the triatomine vector Mepraia spinolai endemic to Chile. We used wild-caught first-to-fourth instar nymphs during cooling (fall and winter) and warming (spring) periods. After capturing, nymphs were fed at the laboratory, and maintained under optimal rearing conditions. Feeding was repeated 40 days later. We followed-up the molting events on 709 nymphs, recording one, two or the absence of molts after two feeding opportunities. Within the same climatic period, only infected second- and fourth-instar nymphs from the warming period showed a larger proportion of double molting compared to uninfected nymphs. Regarding the climatic period, infected and uninfected first- and fourth-instar nymphs exhibited a larger proportion of double molting in the warming and cooling periods, respectively. The pattern of non-molting nymph occurrence suggests they probably reach diapause by environmental stochasticity. The effect of the climatic period and T. cruzi infection on the development of M. spinolai is an instar-dependent phenomenon, highlighting the occurrence of finely synchronized processes at different moments of the life cycle of such an hemimetabolous insect as triatomines. Full article

Triatoma bassolsae, T. longipennis, T. mazzottii, T. pallidipennis, T. phyllosoma and T. picturata are species that have great epidemiological importance in the transmission of Chagas disease in Mexico. However, there is no consensus regarding the specific status of these species, since they appear in various articles as species, subspecies and even subgenera. Thus, we revisited genetic, taxonomic and evolutionary data that allowed us to assess and discuss the specific status of these six species of the T. phyllosoma subcomplex. Phylogenetic studies were performed with nuclear (18S, 28S, ITS-2) and mitochondrial (16S, cytb, COI, COII, 12S) markers deposited in GenBank. In addition, data from experimental crosses were pooled and the genetic distance to the cytb gene was calculated. The phylogenetic reconstruction enabled us to rescue the six species as independent lineages. Post-zygotic reproductive isolation barriers (sterility and/or hybrid collapse) were observed for some experimental crosses. Although the other experimental crosses did not allow us to characterize reproductive barriers, these species showed high genetic distances in relation to the cytb gene (ranging from 4.6% to 14.9%). Thus, based on the revisited literature data, we confirmed the specific status of these six species of the T. phyllosoma subcomplex based on the phylogenetic and biological concepts of the species. Full article

Triatomine bugs of the genera Triatoma and Rhodnius are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by Trypanosoma cruzi. Triatoma virus (TrV), a natural pathogen of Triatoma infestans, has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for in vitro propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of T. infestans (TIE/LULS54) and Rhodnius prolixus (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect R. prolixus, indicating that these new cell lines could be used for propagation of TrV. Full article