Search Results (5)

To maintain the continuous genetic variation and increase the genetic gain, appreciable germplasm diversity and its comprehensive characterization is necessary to further utilize gene sources for pre-breeding. The diversity of pepper forms, cultivation traditions and diverse fruit usages are typical for Balkan countries. Considering this rich diversity, 21 pepper accessions from the Balkan region were evaluated for morphological, biochemical, and insect resistance traits during 2018 and 2019 at Maritsa Vegetable Crops Research Institute, Plovdiv, Bulgaria. Among the studied accessions, the highest productivity was observed in pumpkin shape K1115 and kapia type K1081 accessions, with 0.74 kg and 0.70 kg per plant, respectively. Concerning fruit quality, the highest total polyphenols and ferric reducing antioxidant power (FRAP) were observed in pumpkin shape K712 (203.44 mg GAE/100 g FW) and K1103A (11.49 µmol Fe²⁺/g FW) accessions, respectively. Concerning insect resistance, 38% of studied accessions showed no infestation of green peach aphid. The kapia type K697 accession was seen as the most reliable resistance source, as it was not infested by aphids and had the least thrips (20% on plants) and cotton bollworm (6.67% on plants and 8.34% on fruit) damage. Based on examined traits, accessions were identified for enhanced fruit quality and promising insect resistance and have been included in further pre-breeding efforts. Full article

Drip irrigation is gaining importance in mitigating the consequences of water scarcity even in regions with abundant rainfall. The transition from surface to subsurface drip irrigation is accompanied by numerous problems. To overcome these issues, shallow subsurface drip irrigation can be potentially used as an effective drought control tool that brings additional benefits compared to conventional surface drip irrigation techniques. This research investigated the effects of different calculations of daily crop water requirements, reference evapotranspiration (ETo), and pan evaporation (Eo) on the yield and water use efficiency of pepper irrigated with a surface and shallow subsurface drip irrigation system. The experiment was conducted in field conditions in the Vojvodina region, the northern part of Serbia. The irrigation scheduling was based on the water balance approach. The calculated evapotranspiration rate was about 400 mm for the pepper growing period, regardless of the calculation method. The highest yield of pepper and evapotranspiration water use efficiency was obtained on the Eo variant with surface drip irrigation. However, irrigation water use efficiency showed no statistical significance concerning the calculation of evapotranspiration and irrigation type. The results indicated that both calculation methods and irrigation types can be used in pepper production, but priority should be given to pan-evaporation-based calculation. Full article

Potato spindle tuber viroid (PSTVd) infects various plants. PSTVd pathogenesis is associated with interference with the cellular metabolism and defense signaling pathways via direct interaction with host factors or via the transcriptional or post-transcriptional modulation of gene expression. To better understand host defense mechanisms to PSTVd infection, we analyzed the gene expression in two pepper cultivars, Capsicum annuum Kurtovska kapia (KK) and Djulunska shipka (DS), which exhibit mild symptoms of PSTVd infection. Deep sequencing-based transcriptome analysis revealed differential gene expression upon infection, with some genes displaying contrasting expression patterns in KK and DS plants. More genes were downregulated in DS plants upon infection than in KK plants, which could underlie the more severe symptoms seen in DS plants. Gene ontology enrichment analysis revealed that most of the downregulated differentially expressed genes in both cultivars were enriched in the gene ontology term photosynthesis. The genes upregulated in DS plants fell in the biological process of gene ontology term defense response. We validated the expression of six overlapping differentially expressed genes that are involved in photosynthesis, plant hormone signaling, and defense pathways by quantitative polymerase chain reaction. The observed differences in the responses of the two cultivars to PSTVd infection expand the understanding of the fine-tuning of plant gene expression that is needed to overcome the infection. Full article

The present study aimed to evaluate the effect of supplementing the diet of laying hens with linseed meal and carotenoids from different sources on egg characteristics, yolk fatty acid and carotenoid profile, and lipid peroxidation. A 4-week experiment was conducted on 168 Lohmann Brown layers (43 weeks of age), assigned to four dietary treatments (42 hens/group; 21 replicate/groups with 2 birds/pen) consisting of a control diet (C) and three diets simultaneously supplemented with 6% linseed meal and 2% dried kapia pepper (E1), 2% dried sea buckthorn pomace (E2) and 2% dried carrot (E3). Every 2 weeks, 18 eggs/group/period were collected randomly from each group and used to determine the egg quality and nutritional parameters. The results showed that dietary linseed meal and carotenoids sources improved egg color, carotenoids’ accumulation in egg yolk and fatty acid profile, especially the n-3 PUFA content. Dietary carotenoids supplementation reduced, n-6/n-3 ratio, cholesterol content of the egg yolk and improved yolk pH, egg thickness and yolk oxidative stability. In conclusion, the use of these sources of carotenoids in the linseed meal enriched diets could be an effective way to improve the nutritional properties of the eggs without affecting their quality and consumer’s safety. Full article

Pepper is the second most important vegetable crop in Bulgarian agriculture and has become the subject of extensive breeding programs that frequently employ induced mutagenesis. The success of breeding programs can be enhanced by the efficient and integral application of different biochemical and molecular methods to characterize specific mutant alleles. On the other hand, identifying new cost-effective methods is important under a limited-resources environment. In this paper we compare the levels of five health-related carotenoid compounds of fruits (α-carotene, β-carotene, lutein, β-cryptoxanthin, zeaxanthin) between a mutant variety Oranzheva kapia (possessing high ß-carotene concentration) and a corresponding initial pepper variety Pazardzhishka kapia 794. Both varieties are intended for fresh consumption. Pepper is a major natural source of β-carotene. It was observed that fruit at both commercial and botanical maturity from mutant variety had greater α-carotene and β-carotene concentrations to the initial variety (7.49 and 1.94 times higher, respectively) meaning that the mutant was superior in fruit quality to the initial genotype. Two hydroxylase enzymes, converting α- and β-carotene to lutein and zeaxanthin, respectively, are known to exist in pepper and are encoded by two genes on chromosomes 3 and 6-CrtZ_chr03 and CrtZ_chr06. The molecular characterization of the mutant variety through locus-specific Polymerase chain reaction amplification, gene cloning and sequencing as well as expression was performed. Our results suggest that the increased ß-carotene accumulation in the mutant variety Oranzheva kapia results from a biosynthetic pathway breakdown due to deletion of CrtZ_chr03 gene. Full article