Search Results (14,661)

Background: Pulmonary arterial pressure is a key parameter for diagnosing cardiovascular and pulmonary diseases. Its measurement through right heart catheterization is considered the gold standard, and it is an invasive procedure that entails significant risks for patients. This has motivated the development of non-invasive techniques based on patient-specific imaging, such as Physics-Informed Neural Networks (PINNs), which integrate clinical measurements with physical models, such as the 1D reduced Navier–Stokes model, enabling biologically plausible predictions with limited data. Methods: This work implements a PINN model that uses velocity and area measurements in the main bifurcation of the pulmonary artery, comprising the main artery and its secondary branches, to predict pressure, velocity, and area variations throughout the bifurcation. The model training includes penalties to satisfy the laws of flow and momentum conservation. Results: The results show that, using 4D Flow MRI images from a healthy patient as clinical data, the pressure estimates provided by the model are consistent with the expected ranges reported in the literature, reaching a mean arterial pressure of 21.5 mmHg. Conclusions: This model presents an innovative approach that avoids invasive methods, being the first study to apply PINNs to estimate pulmonary arterial pressure in bifurcations. In future work, we aim to validate the model in larger populations and confirm pulmonary hypertension cases diagnosed through catheterization. Full article

Pulmonary carcinoids (PCs) are rare tumors, with an incidence ranging from 0.2 to 2 cases per 100,000 population per year. They account for 1–2% of all invasive pulmonary malignancies and represent approximately one-fourth to one-third of all well-differentiated neuroendocrine tumors (NETs) in the body. PCs are generally classified as low- to intermediate-grade malignant tumors, further subdivided into typical carcinoid (TC) and atypical carcinoid (AC), respectively. These tumors exhibit neuroendocrine morphology and differentiation, originating from mature cells of the pulmonary diffuse neuroendocrine system. Traditionally, they are categorized as central or peripheral based on their location relative to the bronchial tree; however, they can arise anywhere within the lung parenchyma. Over 40% of cases may be detected incidentally on a standard chest X-ray, although contrast-enhanced computed tomography (CT) remains the diagnostic gold standard. Surgical resection is the treatment of choice for PCs, with the goal of complete tumor removal while preserving as much healthy lung tissue as possible. In contrast, advanced cases are typically not amenable to surgery, and medical management is focused on controlling hormone-related symptoms and limiting tumor progression. This review aims to provide an overview of the current diagnostic and therapeutic approaches to pulmonary carcinoids. Full article

Ultrahigh dose rate (UHDR) radiotherapy, also known as FLASH radiotherapy (FLASH-RT), has shown potential for increasing tumor control while sparing normal tissue. In parallel, gold nanoparticles (GNPs) have been extensively explored as radiosensitizers due to their high atomic number and ability to enhance the generation of reactive oxygen species (ROS) through water radiolysis. In this study, we investigate the synergistic effects of UHDR electron beams and GNP-mediated radiosensitization using Monte Carlo (MC) simulations based on the Geant4-DNA code. A spherical water phantom with embedded GNPs of varying sizes (5–100 nm) was irradiated using pulsed electron beams (100 keV and 1 MeV) at dose rates of 60, 100, and 150 Gy/s. The chemical yield of ROS near the GNPs was quantified and compared to an equivalent water nanoparticle model, and the yield enhancement factor (YEF) was used to evaluate radiosensitization. Results demonstrated that YEF increased with smaller GNP sizes and at lower UHDR, particularly for 1 MeV electrons. A maximum YEF of 1.25 was observed at 30 nm from the GNP surface for 5 nm particles at 60 Gy/s. The elevated ROS concentration near GNPs under FLASH conditions is expected to intensify DNA damage, especially double-strand breaks, due to increased hydroxyl radical interactions within nanometric distances of critical biomolecular targets. These findings highlight the significance of nanoparticle size and beam parameters in optimizing ROS production for FLASH-RT. The results provide a computational basis for future experimental investigations into the combined use of GNPs and UHDR beams in nanoparticle-enhanced radiotherapy. Full article

The biological production of hydrogen offers a renewable and potentially sustainable alternative for clean energy generation. In Northeast Brazil, depleted oil reservoirs (DORs) present a unique opportunity to integrate biotechnology with existing fossil fuel infrastructure. These subsurface formations, rich in residual hydrocarbons (RH) and native H₂ producing microbiota, can be repurposed as bioreactors for hydrogen production. This process, often referred to as “Gold Hydrogen”, involves the in situ microbial conversion of RH into H₂, typically via dark fermentation, and is distinct from green, blue, or grey hydrogen due to its reliance on indigenous subsurface biota and RH. Strategies include nutrient modulation and chemical additives to stimulate native hydrogenogenic genera (Clostridium, Petrotoga, Thermotoga) or the injection of improved inocula. While this approach has potential environmental benefits, such as integrated CO₂ sequestration and minimized surface disturbance, it also presents risks, namely the production of CO₂ and H₂S, and fracturing, which require strict monitoring and mitigation. Although infrastructure reuse reduces capital expenditures, achieving economic viability depends on overcoming significant technical, operational, and biotechnological challenges. If widely applied, this model could help decarbonize the energy sector, repurpose legacy infrastructure, and support the global transition toward low-carbon technologies. Full article

This study focuses on piezoelectric quartz crystal microbalances (QCMs), widely used in space and military instrumentation, as fundamental components in highly sensitive mass detection devices. In this research, a proper setup was developed to investigate the relationship between clamping preload and crystal resonance, with particular attention to the effects of concentrated loads. The latter ones, not properly addressed in the literature, come from the need to safely clamp QCMs in critical environments, like those experienced during the launch of rockets or payloads. Thus, the study investigates the behaviour of piezoelectric quartz crystals (AT-cut, 10 MHz) with gold electrodes, using a QCMs’ three-pinned mounting system. Measurements showed that the effect of the preload on the frequency variation resulted in a repeatable increase in the crystals’ resonance, increasing the loading, up to three ppm more than the unloaded quartz crystal oscillating frequency. Full article

Marker-based motion capture systems using infrared cameras (IR MoCaps) are commonly employed in biomechanical research. However, their high costs pose challenges for many institutions seeking to implement such systems. This study aims to develop a neural network (NN) model to estimate the digitized coordinates of spherical markers and to establish a lower-cost marker-based motion capture system using RGB cameras. Thirteen participants were instructed to walk at self-selected speeds while their movements were recorded with eight RGB cameras. Each participant undertook trials with 24 mm spherical markers attached to 25 body landmarks (marker trials), as well as trials without markers (non-marker trials). To generate training data, virtual markers mimicking spherical markers were randomly inserted into images from the non-marker trials. These images were then used to fine-tune a pre-trained model, resulting in an NN model capable of detecting spherical markers. The digitized coordinates inferred by the NN model were employed to reconstruct the three-dimensional coordinates of the spherical markers, which were subsequently compared with the gold standard. The mean resultant error was determined to be 2.2 mm. These results suggest that the proposed method enables fully automatic marker reconstruction comparable to that of IR MoCap, highlighting its potential for application in motion analysis. Full article

Background/Objectives: Historically, the treatment of subungual melanoma was based on amputation of the affected digit. However, extended wide local excision of the nail apparatus is now considered the conservative gold standard for in situ or minimally invasive forms. There are many after wide local excision reconstruction techniques, but few studies have objectively compared their results. The objectives were to carry out a systematic review of reconstruction after wide local excision reconstructions in the treatment of subungual melanoma. Methods: This systematic review was conducted following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. An exhaustive search was conducted in the PubMed (Medline), Embase, and Cochrane Library databases, up to July 2025. Articles reporting reconstructions after wide local excision for subungual melanoma of the fingers or toes were included. Clinical, technical, and outcome data were analyzed. Results: The literature review comprised 24 articles on 373 patients, primarily those with in situ subungual melanoma. Reconstruction was most often performed using total skin grafts, sometimes combined with dermal matrices. Some authors used local or free flaps. Few studies used validated functional scores. Local recurrences were significant, affecting 18% of patients and requiring secondary amputation. Conclusions: Nail apparatus reconstructions are primarily indicated for in situ or minimally invasive subungual melanomas. Immediate reconstruction carries a risk of performing the reconstruction over residual tumor tissue, particularly in the case of invasive melanomas. Reconstructive techniques, such as full-thickness skin grafts and the use of dermal matrices, can provide satisfactory functional and aesthetic outcomes. However, objective evaluations of these results remain limited, and better standardization of clinical practice, along with prospective studies, is needed to refine long-term outcome assessment. Full article

Brain natriuretic peptide (BNP) is an important biomarker for the diagnosis and prediction of chronic heart failure (CHF). Aiming at the problems of the low sensitivity and poor portability of traditional BNP detection methods, this study proposes a Surface-enhanced Raman-scattering (SERS) fiber-optic sensor based on a multimode fiber (MMF)–no core fiber (NCF) structure. The sensor achieves BNP detection by significantly amplifying the Raman signal of the toluidine blue (TB) marker through the synergistic effect of NCF’s unique optical transmission modes and localized surface plasmon resonance (LSPR). To optimize the sensor performance, we first investigated the effect of the NCF length on the Raman signal, using Rhodamine 6G (R6G), and determined the optimal structural parameters. Combined with the microfluidic chip integration technology, the antibody–BNP–antibody sandwich structure was adopted, and TB was used as the Raman label to realize the quantitative detection of BNP. Experimental results demonstrate that the detection limit of the sensor is lower than the clinical diagnostic threshold and exhibits stability. The sensor sensitivity can be adjusted by regulating the laser power. With its stability and high portability, this sensor provides a new solution for the early diagnosis of heart failure and demonstrates broad application prospects in biomarker detection. Full article

This study investigates the asymmetric responses of AI and ESG Exchange Traded Funds (ETFs) to geopolitical and financial uncertainty, with a focus on resilience across market regimes. The NASDAQ-100 and MSCI ESG Leaders indices are used as proxies for thematic ETFs, and their dynamic interlinkages are examined in relation to volatility indicators (VIX, GPR), alternative assets (Bitcoin, Ethereum, gold, oil, natural gas), and safe-haven currencies (CHF, JPY). A daily dataset spanning the 2016–2025 period is analyzed using Quantile-on-Quantile Regression (QQR) and Wavelet Coherence (WCO), enabling a granular assessment of nonlinear, regime-dependent behaviors across quantiles. Results reveal that ESG ETFs demonstrate stronger downside resilience under extreme uncertainty, maintaining stability even during periods of elevated geopolitical and financial risk. In contrast, AI-themed ETFs tend to outperform under moderate-risk conditions but exhibit greater vulnerability during systemic stress, reflecting differences in asset composition and investor risk perception. The findings contribute to the literature on ETF resilience and cross-asset contagion by highlighting differential behavior patterns under varying uncertainty regimes. Practical implications emerge for investors and policymakers seeking to enhance portfolio robustness through thematic diversification during market turbulence. Full article

Transvaginal ultrasound-guided follicle aspiration remains the gold standard for oocyte retrieval in assisted reproductive technology (ART). This procedure employs either a single-lumen aspiration needle (SLN) or double-lumen aspiration needle (DLN), both of which are effective modalities for oocyte retrieval. The primary objective of this review is to systematically compare the impact and clinical outcomes associated with the use of SLN versus DLN in women undergoing ART. A systematic literature search was conducted across two databases, PubMed and Google Scholar, encompassing publications from their inception until May 2025, and articles published in English. A total of five studies were included in the final analysis. The oocyte yield and the number of MII oocyte did not differ significantly between the groups. Procedural duration was markedly shorter in the SLN group compared to the DLN group. No significant differences were observed in procedure-related complications across groups. Two randomized controlled trials reported comparable fertilization rates and numbers of high-quality embryos between the two needle types. Additionally, clinical pregnancy rates, ongoing pregnancy rates, and live birth rates were similar between the SLN and DLN groups. In conclusion, the utilization ofan SLN for oocyte retrieval demonstrates comparable efficacy to that of a DLN. Full article

The Eastern Kazakhstan Gold Belt is a major black-shale-hosted gold province in Central Asia where the main types of deposits comprise mineralized zones with auriferous sulfides (micro- and nano-inclusions of gold and refractory gold) and quartz veins with visible gold. The quartz vein deposits are economically less important but may potentially represent the upper parts of bigger ore systems concealed at depth. In this work, the mineralogy of the quartz vein deposits and related wall rock alteration zones was studied using microscopy and SEM-EDS analysis, and the geochemical dispersion of the ore elements in primary alteration haloes was documented utilizing spatial distribution maps and statistical treatment methods. The studied auriferous quartz veins are classified as epizonal black-shale-hosted orogenic gold deposits. The veins generally have linear shapes with an average width of ca. 1 m and length up to 150 m and contain high-grade native gold with minor amounts of sulfides. In supergene oxidation zones, the native gold is closely associated with Fe-hydroxide minerals cementing brecciated zones within the veins. The auriferous quartz veins are usually enclosed by the wall rock alteration envelopes, where two types of alteration are distinguished. Proximal phyllic alteration (sericite-albite-pyrite ± chlorite, Fe-Mg-Ca carbonates, arsenopyrite, and pyrrhotite) develops as localized alteration envelopes, and pervasive carbonation accompanied by chlorite ± sericite and albite is the dominant process in the distal alteration zones. The rocks within the alteration zones are enriched in Au and chalcophile elements, and three groups of chemical elements showing significant positive mutual correlation have been identified: (1) an early geochemical assemblage includes V, P, and Co (±Ni), which are the chemical elements characteristic for black shale formations, (2) association of Au, As, and other chalcophile elements is distinctly overprinting, and manifests the main stage of sulfide-hosted Au mineralization, and (3) association of Bi and Hg (±Sb and U) includes the chemical elements that are mobile at low temperatures, and can be explained by activity of the late-stage hydrothermal or supergene fluids. The chalcophile elements show negative slopes from proximal to distal alteration zones and form overlapping positive anomalies on spatial distribution mono-elemental maps. Thus, the geochemical methods can provide useful tools to delineate the ore elemental associations and to outline reproducible anomalies for subsequent regional gold prospecting. Full article

Background: Gold nanoparticles (AuNPs) have several beneficial properties that make them effective as intracellular drug carriers, and their potential for various diagnostic and therapeutic applications is gaining recognition. Depending on their size and shape, AuNPs can cross the central nervous system (CNS) through the blood–brain barrier (BBB). In the CNS, they can exert a variety of influences on neuronal and glial cells, which can be both supportive—promoting cell health and function—and cytotoxic, potentially leading to cellular damage. The hypothalamus (HT) is the first region where nanoparticles (NPs) interact, as this neuroendocrine center is particularly sensitive to factors in the systemic circulation due to its function and location. This area is affected by systemic factors, including pro-opiomelanocortin (POMC) neurons, which regulate metabolic function and maintain homeostasis. The activity of mitochondria within these cells influences their response to both external factors and the presence of AuNPs, thereby facilitating a complex interplay between nanoparticle interactions and cellular metabolism in this vital brain region. Aims: This study investigates how AuNPs, at different concentrations and exposure times under in vitro conditions, affect the mitochondrial activity of POMC neurons, aiming to provide a comprehensive understanding of the mechanisms in the HT. Methods: The study investigates the effect of varying gold nanoparticle concentrations on the mitochondrial activity of POMC neurons over treatment periods of 1, 15, 24, and 48 h. Mitochondrial activity was measured using a Seahorse XFp Analyzer to provide high-resolution insights. Additionally, mitochondrial functionality was assessed through the detection of reactive oxygen species (ROS) and cell viability. Results: The findings indicated that the effects of gold nanoparticles on mitochondrial activity depend significantly on their concentration and exposure time. Specifically, exposure leads to an increase in early response systems, the citric acid cycle, and proton efflux, ultimately resulting in the inhibition of mitochondrial function and ATP production in POMC cells. This disruption may affect hypothalamic regulation and energy metabolism. Full article

Streptococcus pneumoniae remains a leading cause of invasive diseases, particularly affecting young children and the elderly. Currently, two main types of pneumococcal vaccines are commercially available: polysaccharide vaccine (PPSV23) and conjugate vaccines (e.g., PCV20). Of over 100 identified pneumococcal serotypes, vaccines targeting 24 serotypes covered by PPSV23 and PCV20 (19 serotypes overlap between the two vaccines) have been developed, with serotype distribution varying by geography, age, and time. The immune response to pneumococcal vaccines differs across serotypes, vaccine types (polysaccharide vs. conjugate), and host factors. Quantitative methods for antibody assessment—particularly newer high-throughput assays—have emerged since 2000 to address limitations in conventional approaches. However, these methods have not been comprehensively reviewed. This scoping review aimed to systematically map the existing literature on quantitative methods used to assess antibody responses to pneumococcal vaccines. Specific objectives included the following: 1. summarizing conventional and novel quantitative immunoassays; 2. evaluating the current state of validation and application of these methods; 3. identifying knowledge gaps and methodological challenges. We followed the PRISMA-ScR guidelines. We included the following: 1. peer-reviewed, open-access papers related to immunoassays used for pneumococcal antibody assessment; 2. articles written in English; 3. Studies published between 2000 and 2023. We excluded the following: 4. studies focusing on other pathogens, employing different analytical methods, or using animal models. Articles meeting the eligibility criteria were primarily retrieved from PubMed and Scopus. If free full-text versions were unavailable there, Google Scholar or the original journal databases were consulted. All references were exported to EndNote 20 for further management. At the beginning of the review, a data-charting form was developed based on prior studies and commonly addressed themes. Additional charts were created to accommodate newly identified variables during the review. All charting tools were reviewed and finalized through discussion among all research team members. The included studies were classified into five thematic groups: 1. general descriptions of quantitative assessment methods, 2. assay development and validation, 3. comparative studies, 4. technical details of assay development, 5. interpretation of assay application findings. Of 1469 articles from PubMed and 2946 articles from Scopus initially identified, 55 articles met the inclusion criteria. The earliest methods included radioimmunoassays, later replaced by WHO-standardized ELISA. While ELISA remains the gold standard, it is limited by labor, cost, and throughput. Multiplex immunoassays (MIAs), including Luminex-based platforms, have demonstrated advantages in efficiency and scalability. However, many MIAs did not initially meet WHO validation criteria. More recent assays show an improved performance, yet interlaboratory variability and lack of standardized protective thresholds remain major limitations. This review provides the first comprehensive mapping of quantitative antibody assessment methods for pneumococcal vaccines. Although ELISA continues to serve as the benchmark, MIAs represent a promising next-generation approach. Continued efforts are needed to harmonize assay validation protocols and establish global standards for protective thresholds, which will enhance the reliability of vaccine efficacy monitoring across diverse populations. Full article

There is a gap in the research on LEED-certified projects that has arisen from combining the “old” LEED system and “new” LEED version in green building practice. This study is focused on gold-certified office projects under LEED for Existing Buildings version 4.1 (LEED-EB v4.1). Wilcoxon–Mann–Whitney and Cliff’s δ tests were used to conduct a pairwise comparison of six countries (Sweden, Ireland, Germany, Spain, Italy, and Israel) in terms of five performance indicators (transportation, water, energy, waste, and indoor environmental quality). The results show that Sweden and Germany outperformed Italy (p = 0.002 and 0.018, respectively) in transportation performance. Ireland outperformed Italy and Israel (p = 0.015 and 0.032, respectively), and Germany outperformed Italy and Israel (p = 0.003 and 0.009, respectively) in water performance. Germany outperformed Sweden, Ireland, and Israel (p < 0.001, respectively) and Sweden, Spain, and Italy outperformed Israel (p < 0.001, p = 0.008, and p = 0.009, respectively) in energy performance. Italy outperformed Sweden, Ireland, Germany, and Israel (0.001 < p ≤ 0.013) and Spain outperformed Germany and Israel (p = 0.015 and p < 0.001, respectively) in waste performance. Israel outperformed Sweden, Germany, and Italy (p < 0.001, p < 0.001, and p = 0.006, respectively) and Spain, Ireland, and Italy outperformed Sweden (p < 0.001, p = 0.002, and p = 0.004, respectively) in indoor environmental quality performance. The findings of this study show that each of the six selected countries has an individual LEED-EB v4.1 certification strategy. This study contributes new knowledge that can support LEED professionals in developing LEED certification strategies for each country. Full article

Eosinophilic myocarditis (EM) is a rare and potentially fatal form of acute myocarditis. Currently, no validated diagnostic criteria exist, and definitive diagnosis relies on endomyocardial biopsy (EMB) not devoid of periprocedural complications. This review aims to explore how a multimodality imaging approach can support early diagnosis, reduce reliance on EMB, enable risk stratification and monitor the response to anti-inflammatory therapy. In particular, while echocardiography provides rapid and useful information in suspected EM, cardiac magnetic resonance (CMR) remains the non-invasive gold standard for diagnosis due to its ability to provide accurate tissue characterization. Moreover, positron emission tomography/computed tomography (PET/CT) and cardiac CT (CCT) may offer valuable insights, particularly when echocardiographic image quality is poor or CMR is contraindicated or unavailable. Based on our experience and current literature, an optimal multimodality imaging approach should reserve EMB only for high-risk or inconclusive cases. Furthermore, this strategy offers complementary information, supporting clinical decisions and optimizing long-term outcomes. Full article