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Keywords = fourth cleaning stage

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17 pages, 3928 KB  
Article
Ammonia Stress Induces Transcriptional Expression Changes in the Mature Eggs of the Acipenser baerii
by Qian Qi, Cheng Zhang, Wenhua Wu, Qi Zhou, Chenran Lv, Xiaohui Sun and Feng Yang
Animals 2025, 15(21), 3122; https://doi.org/10.3390/ani15213122 - 28 Oct 2025
Viewed by 901
Abstract
Ammonia is a key factor in the water, impacting the physiological functions of aquatic organisms. To explore the effect of ammonia stress on mature eggs, female A. baerii at the end of the fourth stage of ovarian development were subjected to varying ammonia [...] Read more.
Ammonia is a key factor in the water, impacting the physiological functions of aquatic organisms. To explore the effect of ammonia stress on mature eggs, female A. baerii at the end of the fourth stage of ovarian development were subjected to varying ammonia concentrations (0 mg/L (control, C), 10 mg/L (low concentration, T1), and 50 mg/L (high concentration, T2)) for 96 h. After 96 h of stress, histological analysis revealed that the follicular membranes of group T1 remained intact and clear compared to group C, although the vacuole fusion had begun. In contrast, the T2 group exhibited ruptured follicular membranes and adhered yolk granules compared to the C group, indicating structural damage. Transcriptome analysis generated 97.89 Gb of clean data, with each sample yielding over 6.09 Gb. A total of 5576, 3719, and 9446 differentially expressed genes (DEGS) were screened from T1 vs. C, T2 vs. C, and T2 vs. T1 comparisons, respectively. Gene Ontology analysis (GO) functional enrichment analysis showed that DEGS were significantly enriched in multicellular organism processes (T1 vs. C), cell surface receptor signaling pathways (T2 vs. C), and immune system processes (T2 vs. T1) during biological processes. It indicates that ammonia exposure may enrich cellular components in the extracellular space, potentially disrupting the function of the extracellular matrix. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment indicated significant impacts on amino acid metabolism, particularly glutamate and arginine pathways, as well as key pathways involved in steroid biosynthesis and antioxidation. Weighted gene co-expression network analysis (WGCNA) revealed that a total of 26,369 DEGs were divided into 29 distinct modules, displaying obvious associations with their traits. In the T2 vs. C group, antioxidation-related genes such as GST and GCLM were significantly downregulated, and the expressions of key enzymes for steroid synthesis, such as CYP11A1, CYP17, and CYP19A1 were suppressed, indicating that high ammonia nitrogen concentrations impair oocyte function by inducing oxidative stress and disrupting hormone synthesis. This study provides a comprehensive repertoire of candidate genes associated with ammonia stress in the mature egg of A. baerii, which will be useful for development of sturgeon breeding and reproduction. Full article
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14 pages, 4168 KB  
Article
Comparative Long-Term Monitoring of Microplastics in the Effluent of Three Different Wastewater Treatment Plants with Two, Three, and Four Treatment Stages
by Michael Toni Sturm, Daphne Argyropoulou, Erika Myers, Anika Korzin, Pieter Ronsse, Oleg Zernikel, Dennis Schober and Katrin Schuhen
Water 2025, 17(5), 711; https://doi.org/10.3390/w17050711 - 28 Feb 2025
Cited by 6 | Viewed by 2634
Abstract
Wastewater treatment plants (WWTPs) are important point sources for microplastics (MPs) in the environment. For effective mitigation measures and regulations, it is important to monitor their release into the environment and understand the level of MPs in the WWTP effluents based on different [...] Read more.
Wastewater treatment plants (WWTPs) are important point sources for microplastics (MPs) in the environment. For effective mitigation measures and regulations, it is important to monitor their release into the environment and understand the level of MPs in the WWTP effluents based on different treatment technologies. In this study, we compare the MP levels in the effluents of three different municipal WWTPs which each use a different treatment concept: a conventional three-stage WWTP, one with an additional fourth cleaning stage using powdered activated carbon, and a two-stage WWTP utilizing a membrane bioreactor (MBR). Long-term monitoring was performed on the WWTP effluents using the same standardized methods for sample collection, preparation, and detection, based on fluorescent staining. Despite the various advanced treatment processes, there are no significant differences in the resulting MP contamination in the investigation of WWTP effluents. The average MP concentrations in the effluents were 21.8 MPs/L for the conventional three-stage WWTP, 15.1 MPs/L for the four-stage WWTP, and 15.1 MPs/L for the MBR. Further, the MP contamination in all effluents shows a strong fluctuation over time. These findings highlight the need for standard MP monitoring at WWTPs, to gain a better understanding of the MP emission in different treatment processes. Further, it highlights the need for a fourth treatment stage that specifically targets MP removal to effectively prevent the MP release from WWTPs into the environment. Full article
(This article belongs to the Special Issue Microplastics Pollution in Aquatic Environments)
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23 pages, 2725 KB  
Article
What Kind of Relationship Between Government and Business Can Stimulate Regional Green Innovation Development?—Analysis Based on Mixed Methods
by Xiaotian Ji, Xiaobao Peng and Sihan Hou
Sustainability 2025, 17(3), 993; https://doi.org/10.3390/su17030993 - 26 Jan 2025
Cited by 3 | Viewed by 2897
Abstract
China’s economic development has had adverse effects on the environment. Nevertheless, the Chinese government is optimistic about its ability to achieve a harmonious balance between economic growth and environmental sustainability. To this end, the government promotes, guides, and supports green innovation through the [...] Read more.
China’s economic development has had adverse effects on the environment. Nevertheless, the Chinese government is optimistic about its ability to achieve a harmonious balance between economic growth and environmental sustainability. To this end, the government promotes, guides, and supports green innovation through the provision of policies and the creation of a conducive environment. Enterprises play a vital role in implementing and advancing green innovation, making them essential to realizing green development through their innovative capabilities. The relationship between government and business acts as a bridge between these two entities, fostering a synergistic effect that is crucial for achieving this objective. This paper conducts a comprehensive analysis of the existing literature on government–business relationships and green innovation, both domestically and internationally. Utilizing data envelopment analysis, it accurately measures the green innovation efficiency of 292 prefectural-level cities in China. The paper then employs a combination of a qualitative comparative analysis and a requisite condition analysis to establish the causal link between government–business relationships and green innovation development. Our detailed analysis of the data has identified three modes of driving green innovation: ‘government service-oriented’, ‘policy support-oriented’, and ‘pro- and clean-oriented’. These modes correspond to the developmental trajectories of China’s first, second, and third–fourth–fifth-tier cities, respectively. This paper offers policy recommendations for the systematic reform and optimization of government–business relationships in China. These recommendations are grounded in the three dimensions of the government–business relationship and consider the current conditions of cities at various stages of economic development. The findings and recommendations presented in this paper can serve as valuable insights for policymakers and businesses alike, providing guidance for future initiatives aimed at fostering a more sustainable economy. Full article
(This article belongs to the Section Sustainable Urban and Rural Development)
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24 pages, 6714 KB  
Review
Research Perspectives on Buildings’ Sustainability after COVID-19: Literature Review and Analysis of Changes
by Ugo Maria Coraglia, Davide Simeone and Marco Alvise Bragadin
Buildings 2024, 14(2), 482; https://doi.org/10.3390/buildings14020482 - 8 Feb 2024
Cited by 3 | Viewed by 2990
Abstract
The concept of sustainability introduced in 1987 has characterised the world of research in the AEC (Architecture, Engineering, and Construction) sector, directing it to try to tackle the problems inherent to the impacts of climate change on buildings. In [...] Read more.
The concept of sustainability introduced in 1987 has characterised the world of research in the AEC (Architecture, Engineering, and Construction) sector, directing it to try to tackle the problems inherent to the impacts of climate change on buildings. In addition, the advent of COVID-19, disrupting the world and people’s habits, has obviously also strongly affected the academic world. Indeed, this paper aims to analyse the changes and differences between the pre- and post-pandemic periods through a two-stage hybrid literature review. First, through a systematic literature review, 90 articles were selected, filtered through different keywords (sustainability, climate, architecture, and buildings). Once the reasoned selection of papers had been made, it was possible to proceed to the second phase, which involved a thematic analysis according to the three classic macro themes of sustainability (Environment, Social, and Economic) and the inclusion of a fourth macro theme (Management and Development), which made it possible to reintroduce a holistic view of the problem. The results of this literature review revealed the presence of a growing interest in the topic analysed and a more homogenous distribution across all continents, with no distinction between developed and developing countries. Economic sustainability interests emerge post-COVID-19 through the deepening of the themes of clean energy and direct control of consumption. At the level of Environmental sustainability, on the other hand, COVID-19 replaced the house at the centre of people’s lives. In addition, the Management and Development macro theme highlighted the need for an integrated view of the problem and the unexpected return of researchers preferring new constructions, which, despite alternative green approaches and renovations, can guarantee better performance. Full article
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23 pages, 6860 KB  
Article
Adaptable Process Design as a Key for Sustainability Upgrades in Wastewater Treatment: Comparative Study on the Removal of Micropollutants by Advanced Oxidation and Granular Activated Carbon Processing at a German Municipal Wastewater Treatment Plant
by Michael Toni Sturm, Erika Myers, Dennis Schober, Clara Thege, Anika Korzin and Katrin Schuhen
Sustainability 2022, 14(18), 11605; https://doi.org/10.3390/su141811605 - 15 Sep 2022
Cited by 22 | Viewed by 9647
Abstract
Micropollutants have been increasingly detected at low concentrations in surface waters and may have harmful effects on humans, organisms, and the environment. As wastewater treatment plants are one of the main sources of micropollutants, conventional wastewater treatment methods and plants (mainly one to [...] Read more.
Micropollutants have been increasingly detected at low concentrations in surface waters and may have harmful effects on humans, organisms, and the environment. As wastewater treatment plants are one of the main sources of micropollutants, conventional wastewater treatment methods and plants (mainly one to three cleaning stages) must be improved through an advanced (fourth) treatment stage. The optimal fourth treatment stage should be determined based not only on removal efficiencies but also on a holistic sustainability assessment that further considers the process’s adaptability, economic, environmental, and social parameters. The ability of a tertiary wastewater treatment plant to remove organic pollutants was investigated over four months using two different advanced treatment methods: (1) an advanced oxidation process (AOP) (using UV + H2O2) and (2) granular activated carbon (GAC). The resulting average micropollutant removal efficiencies were 76.4 ± 6.2% for AOP and 90.0 ± 4.6% for GAC. As the GAC became saturated, it showed a decreasing performance from 97.6% in week one to 80.7% in week 13, after 2184 bed volumes were processed. For the AOP, adjusting the UV and H2O2 doses results in higher removal efficiencies. With 40 ppm H2O2 and 10 kJ/m2 UV, a removal of 97.1% was achieved. Furthermore, the flexibility and adaptability of the AOP process to adjust to real-time water quality, along with a lower resource consumption and waste disposal, make it a more promising technology when comparing the sustainability aspects of the two methods. Full article
(This article belongs to the Section Sustainable Engineering and Science)
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33 pages, 4665 KB  
Article
Comparative Analysis of Five Widely-Used Multi-Criteria Decision-Making Methods to Evaluate Clean Energy Technologies: A Case Study
by Saeed Khojaste Effatpanah, Mohammad Hossein Ahmadi, Pasura Aungkulanon, Akbar Maleki, Milad Sadeghzadeh, Mohsen Sharifpur and Lingen Chen
Sustainability 2022, 14(3), 1403; https://doi.org/10.3390/su14031403 - 26 Jan 2022
Cited by 59 | Viewed by 6574
Abstract
Over the last decade, the total primary energy consumption has increased from 479 × 1015 BTU in 2010 to 528 × 1015 BTU in 2020. To address this ever-increasing energy demand, as well as prevent environmental pollution, clean energies are presented [...] Read more.
Over the last decade, the total primary energy consumption has increased from 479 × 1015 BTU in 2010 to 528 × 1015 BTU in 2020. To address this ever-increasing energy demand, as well as prevent environmental pollution, clean energies are presented as a potential solution. In this regard, evaluating and selecting the most appropriate clean energy solution for a specific area is of particular importance. Therefore, in this study, a comparative analysis in Jiangsu province in China was performed by describing and implementing five prominent multi-criteria decision-making methods in the field of energy technology selection, including SAW, TOPSIS, ELECTRE, VIKOR, and COPRAS. The decision problem here consists of four clean energy options, including solar photovoltaic, wind, nuclear, and biomass, which have been evaluated by twelve basic and important criteria for ranking clean energy options. The obtained results, according to all five MCDM methods, indicate that solar photovoltaic was the optimal option in this study, followed by wind energy. The nuclear and biomass options placed third and fourth, respectively, except in the ELECTRE method ranking, in which both options scored the same and thus neither was superior. Finally, by conducting a comprehensive two-stage sensitivity analysis, in the first stage, it was found that changes in the weights of land use and water consumption criteria had the greatest impact on the performance of options, among which biomass and nuclear showed high sensitivity to variations in criteria weights. In the second stage, by defining five scenarios, the ranking of options was evaluated from different aspects so that the decision maker/organization would be able to make appropriate decisions in different situations. Full article
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14 pages, 5406 KB  
Article
Gradient Cleaning Method of Potato Based on Multi-Step Operation of Dry-Cleaning and Wet Cleaning
by Hongguang Yang, Jianchun Yan, Hai Wei, Huichang Wu, Shenying Wang, Longlong Ji, Xiaowei Xu and Huanxiong Xie
Agriculture 2021, 11(11), 1139; https://doi.org/10.3390/agriculture11111139 - 13 Nov 2021
Cited by 4 | Viewed by 6181
Abstract
In view of the poor effectiveness of existing potato cleaning methods in China and reflecting the findings of a research analysis of basic sizes and types of impurities on potato tubers, a gradient cleaning method for potato based on a multi-step dry-cleaning and [...] Read more.
In view of the poor effectiveness of existing potato cleaning methods in China and reflecting the findings of a research analysis of basic sizes and types of impurities on potato tubers, a gradient cleaning method for potato based on a multi-step dry-cleaning and wet cleaning operation was proposed. The method mainly consists of dry-cleaning and wet cleaning. The dry-cleaning stage, which combines vibration and brushing, could effectively remove impurities such as residual rhizomes, peeled potato skin, and large pieces of soil and crushed stone from the surface of potato tubers. The wet cleaning stage adopts the gradient cleaning method of pre-cleaning, rough cleaning and fine cleaning, which could further remove soil and crushed stone attached to the surface and hidden in the sprout eyes of potato tubers. The optimal parameter combination for the gradient cleaning method was determined as follows. The potato feeding amount was 3 t/h, the speed of the rubber chain rod mechanism was 25 r/min, the speed of the first and third brush roller was 40 r/min, the speed of the second and fourth brush roller was 56 r/min, the moving speed of the immersion mechanism conveying net chain was 0.04 m/s, the speed of the brush roller in the high pressure spray and brush roller combination mechanism was 40 r/min, the ultrasonic power was 1200 W, the ultrasonic frequency was 33 kHz, the bubble intensity was 300 L/min, and the moving speed of the conveying net chain in the ultrasonic and bubble combination mechanism was 0.05 m/s. Taking the impurity removal rate and damage rate of potato tuber as the test indexes, a potato cleaning performance test was carried out under the optimal parameters combination. The results showed that the average impurity removal rate and damage rate of potato tubers were 99.05% and 2.48%, respectively. Additionally, the operational performance fully met the requirements for potato cleaning. This study provides a new method for potato cleaning in China and can also provide a reference for cleaning other root and tuber crops. Full article
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21 pages, 11685 KB  
Article
Improving the Quality of Citizen Contributed Geodata through Their Historical Contributions: The Case of the Road Network in OpenStreetMap
by Afsaneh Nasiri, Rahim Ali Abbaspour, Alireza Chehreghan and Jamal Jokar Arsanjani
ISPRS Int. J. Geo-Inf. 2018, 7(7), 253; https://doi.org/10.3390/ijgi7070253 - 28 Jun 2018
Cited by 26 | Viewed by 8149
Abstract
OpenStreetMap (OSM) has proven to serve as a promising free global encyclopedia of maps with an increasing popularity across different user communities and research bodies. One of the unique characteristics of OSM has been the availability of the full history of users’ contributions, [...] Read more.
OpenStreetMap (OSM) has proven to serve as a promising free global encyclopedia of maps with an increasing popularity across different user communities and research bodies. One of the unique characteristics of OSM has been the availability of the full history of users’ contributions, which can leverage our quality control mechanisms through exploiting the history of contributions. Since this aspect of contributions (i.e., historical contributions) has been neglected in the literature, this study aims at presenting a novel approach for improving the positional accuracy and completeness of the OSM road network. To do so, we present a five-stage approach based on a Voronoi diagram that leads to improving the positional accuracy and completeness of the OSM road network. In the first stage, the OSM data history file is retrieved and in the second stage, the corresponding data elements for each object in the historical versions are identified. In the third stage, data cleaning on the historical datasets is carried out in order to identify outliers and remove them accordingly. In the fourth stage, through applying the Voronoi diagram method, one representative version for each set of historical versions is extracted. In the final stage, through examining the spatial relations for each object in the history file, the topology of the target object is enhanced. As per validation, a comparison between the latest version of the OSM data and the result of our approach against a reference dataset is carried out. Given a case study in Tehran, our findings reveal that the completeness and positional precision of OSM features can be improved up to 14%. Our conclusions draw attention to the exploitation of the historical archive of the contributions in OSM as an intrinsic quality indicator. Full article
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