Search Results (215)

Balantioides coli is the only ciliate currently described as an intestinal parasite of humans, although it can also infect other animals, particularly pigs. Its in vitro cultivation remains challenging, and no axenic culture system is currently available. Cultures are initiated by adding small amounts of feces containing cysts or trophozoites to the culture medium. Implantation success is lower when starting from cysts, and the mechanisms and early events of excystation remain poorly understood. In this study, we describe the sequence of events involved in excystation and identify factors potentially important for culture establishment. Cysts were obtained from orangutan feces and genetically confirmed as B. coli. Only viable cysts, determined by trypan blue or methylene blue exclusion, were used. After artificial digestion with pepsin and trypsin, cysts were incubated at 28 °C for up to 72 h in DMEM supplemented with L-glutamine, yeast extract, fetal bovine serum, and starch granules. Excystation began with a fissure in the cyst wall, allowing for bacterial entry. This appeared to stimulate the trophozoites, the increased motility of which progressively weakened and ruptured the wall, allowing for their emergence. Wall rupture and bacterial entry were critical for activation., whereas starch type had no apparent influence. Excystation occurred within the first hours; otherwise, cysts degenerated. Full article

The efficacy of water resource management and protection hinges on a profound understanding of the controlling factors and regulatory mechanisms that shape groundwater chemistry within aquifers. Despite this, our comprehension of how groundwater chemistry and ion sources vary across diverse aquifer types remained limited. To bridge this gap, our study conducted a detailed hydrochemical and statistical investigation of porous, fissured, and karst aquifers. By applying multivariate statistical techniques, including principal component analysis (PCA) and hierarchical cluster analysis (HCA), the hydrochemical characteristics and main ion sources of each aquifer type, as well as distinct controlling factors and regulation patterns, were determined. Notably, evaporation predominantly affected the hydrochemistry of porous aquifers, whereas mineral dissolution and rock weathering processes played a pivotal role in shaping the groundwater evolution of fissured and karst aquifers. HCO₃⁻ and SO₄²⁻ are the most common anions of all types, while Na⁺ is dominant in porous and fissured aquifers and Ca²⁺ is dominant in karst aquifers. The most common hydrochemical types identified were HCO₃-Ca·Mg (accounting for approximately 56.84%) and SO₄·Cl-Na (constituting approximately 21.75%). PCA results revealed that lateral recharge from fissured aquifers in hilly regions into the groundwater of porous aquifer, and wastewater discharge and agricultural fertilizer application, significantly impact the groundwater chemistry across all three aquifer types. It is worth noting that the dissolution of carbonate minerals, often influenced by human activities, had a profound effect on the hydrochemistry of each aquifer. Conversely, the dissolution of evaporitic minerals affected groundwater chemistry primarily through cation exchange processes. In summary, the hydrochemical characteristics of these aquifer types were predominantly shaped by a complex interplay of mineral dissolution, cation exchange, evaporation, and anthropogenic activities, with notable contributions from fissured aquifer recharge and pollution. These insights were critical for informing national-level strategies for groundwater resource protection and management. Full article

This study systematically unravels the hydrochemical evolution mechanisms and driving forces in multi-aquifer systems of Qingdao, a coastal economic hub. Integrated hydrochemical analysis of porous, fissured, and karst water, combined with PHREEQC modeling and Positive Matrix Factorization (PMF), deciphers water–rock interactions and anthropogenic perturbations. Groundwater exhibits weak alkalinity (pH 7.2–8.4), with porous aquifers showing markedly higher TDS (161.1–8203.5 mg/L) than fissured (147.7–1224.8 mg/L) and karst systems (361.1–4551.5 mg/L). Spatial heterogeneity reveals progressive hydrochemical transitions (HCO₃-Ca → SO₄-Ca·Mg → Cl-Na) in porous aquifers across the Dagu River Basin. While carbonate (calcite) and silicate weathering govern natural hydrochemistry, evaporite dissolution and seawater intrusion drive severe groundwater salinization in the western Pingdu City and the Dagu River Estuary (localized TDS up to 8203.5 mg/L). PMF source apportionment identifies acid deposition-enhanced dissolution of carbonate/silicate minerals, with nitrate contamination predominantly sourced from agricultural runoff and domestic sewage. Landfill leachate exerts pronounced impacts in Laixi and adjacent regions. This study offering actionable strategies for salinity mitigation and contaminant source regulation, thereby providing a scientific framework for sustainable groundwater management in rapidly urbanizing coastal zones. Full article

In this study, sulfur-doped biochar (SBC) was successfully synthesized using peanut shells as the raw material and sulfur powder as the sulfur source. The composition, structural characteristics, and catalytic performance of SBC in the degradation of phenol via persulfate (PDS) activation were systematically investigated. Characterization results demonstrate that the prepared SBC exhibited a typical lamellar structure with abundant pores and fissures on its surface. XPS analysis confirmed the successful incorporation of sulfur into the biochar matrix, primarily in the form of thiophene. Under the optimized condition of a 20% sulfur doping ratio, the SBC exhibited high efficiency in activating PDS, achieving a phenol degradation rate of 97%. Remarkably, the removal rate remained at 81% even after the fifth cycle, indicating excellent cyclic stability. Density functional theory (DFT) calculations and electrochemical impedance spectroscopy (EIS) measurements further revealed that sulfur doping significantly modified the electron density distribution of the biochar, reducing its surface electrochemical impedance from 32.88 Ω to 13.64 Ω. This reduction facilitated efficient electron transfer during the catalytic process. This study provides both experimental and theoretical insights into the charge distribution characteristics of sulfur-doped biochar, offering valuable references for understanding the mechanism of PDS activation by SBC. Full article

In cold-region rock engineering, freeze–thaw cycle-induced crack propagation in fractured rock masses serves as a major cause of disasters such as slope instability. Existing studies primarily focus on the influence of individual fissure parameters, yet lack a systematic analysis of the crack propagation mechanisms under the coupled action of multiple parameters. To address this, we establish three groups of slope models with different rock bridge distances (d), rock bridge angles (α), and fissure angles (β) based on the PFC2D discrete element method. Frost heave loads are simulated by incorporating the volumetric expansion during water–ice phase change. The Parallel Bond Model (PBM) is used to capture the mechanical behavior between particles and the bond fracture process. This reveals the crack evolution laws under freeze–thaw cycles. The results show that, at a short rock bridge distance of d = 60 m, stress concentrates in the fracture zone. This easily leads to the rapid penetration of main cracks and triggers sudden instability. At a long rock bridge distance where d ≥ 100 m, the degree of stress concentration decreases. Meanwhile, the stress distribution range expands, promoting multiple crack initiation points and the development of branch cracks. The number of cracks increases as the rock bridge distance grows. In cases where the rock bridge angle is α ≤ 60°, stress is more likely to concentrate in the fracture zone. The crack propagation exhibits strong synergy, easily forming a penetration surface. When α = 75°, the stress concentration areas become dispersed and their distribution range expands. Cracks initiate earliest at this angle, with the largest number of cracks forming. Cumulative damage is significant under this condition. When the fissure angle is β = 60°, stress concentration areas gather around the fissures. Their distribution range expands, making cracks easier to propagate. Crack propagation becomes more dispersed in this case. When β = 30°, the main crack rapidly penetrates due to stress concentration, inhibiting the development of branch cracks, and the number of cracks is the smallest after freeze–thaw cycles. When β = 75°, the freeze–thaw stress dispersion leads to insufficient driving force, and the number of cracks is 623. The research findings provide a theoretical foundation for assessing freeze–thaw damage in fractured rock masses of cold regions and for guiding engineering stability control from a multi-parameter perspective. Full article

Slope failures in overconsolidated hard clays present significant geotechnical challenges, particularly in stratified formations prone to pre-existing discontinuities. Despite extensive research on residual shear strength and fissuring in stiff clays, the role of undetected tension cracks and their interaction with hydrogeological conditions in temporary excavations remains underexplored. This study addresses this research gap through a detailed case study of a slope failure during an unsupported residential excavation on Corfu Island, Greece. The investigation aimed to identify the failure mechanism, assess the influence of geological discontinuities and groundwater conditions, and evaluate the contribution of residual shear strength to slope stability. The methodology combined field observations, laboratory testing (including unconfined compression and ring shear tests), and numerical modelling using both finite element (FEM) and limit equilibrium (LEM) approaches. The results revealed that a nearly vertical, pre-existing fissure—acting as a tension crack—and water infiltration along the clay–sandstone interface significantly reduced the factor of safety, triggering a planar slide. Both FEM and LEM analyses indicated that critical conditions for failure were reached with a residual friction angle of 19°, inclined sandstone layers at 15–17°, and hydrostatic pressure from groundwater accumulation. This study demonstrates the compounded destabilizing effects of undetected discontinuities and water pressures in stratified hard clays and underscores the necessity of comprehensive geotechnical assessments for temporary excavations, even in seemingly stable formations. Full article

Taking the coastal area of Binhai County, Jiangsu Province, as an example, this study first investigated the basic natural geography and the regional geological and hydrogeological conditions of the study area, and then carried out in-depth geophysical prospecting, hydrogeological tests, geothermal temperature monitoring, hydrochemistry and isotope analyses, and other studies based on the results to comprehensively and systematically reveal the genesis mechanism of the geothermal water resources of this coastal area from multiple perspectives. The results showed the following: the geothermal water in this area mainly comes from atmospheric precipitation; the deep east–northwest interlaced fracture is the recharge and transportation channel; the Cambrian–Ordovician carbonate rock layer, enriched by the development of cavernous fissures, forms the thermal storage layer; the underground heat mainly comes from the upward heat flow along the deep fracture and the natural warming of the strata; and the thermal reservoir cover comprises Paleozoic and Mesozoic clastic rocks that have a high mud content and form a thick layer. The genesis mode of this area is as follows: the atmospheric precipitation infiltrates and is recharged through the exposed alpine carbonate fissures in the Lianyungang area, and then it is transported to the south along the large deep fracture under the action of a high hydraulic pressure head; meanwhile, it is heated by the heat flow in the deep part of the fracture and water–rock interactions with the strata occur. Geothermal water with a calculated thermal storage temperature of 83.6 °C is formed at a depth of 2.9 km, which is blocked by the intersection of the northeast and northwest fractures to form a stagnant zone in the coastal area. Full article

Fractures and voids are widely distributed in slope rock masses. These defects promote crack initiation and propagation, ultimately leading to rock mass failure. Investigating their damage evolution mechanisms and strength characteristics is of significant importance for slope hazard prevention. A numerical simulation study of Brazilian splitting tests on disk samples containing prefabricated holes and fractures was conducted using the Finite Element Method with Cohesive Zone Modeling (FEM-CZM) in ABAQUS by embedding zero-thickness cohesive elements within the finite element model. This 2021 study analyzed the effects of fracture angle and length on tensile strength and crack propagation characteristics. The results revealed that when the fracture angle is small, cracks initiate near the fracture and propagate and intersect radially as the load increases, ultimately leading to specimen failure, with the crack coalescence pattern exhibiting local closure. As the fracture angle increases, the initiation location of the crack shifts. With an increase in fracture length, the crack initiation position may transfer to other parts of the fracture or near the hole, and longer fractures may result in more complex coalescence patterns and local closure phenomena. During the tensile and stable failure stages, the load–displacement curves of samples with different fracture angles and lengths exhibit similar trends. However, the fracture angle has a notable impact on the curve during the shear failure stage, while the fracture length significantly affects the peak value of the curve. Furthermore, as displacement increases, the proportion of tensile failure undergoes a process of rapid decline, slow rise, and then rapid decline again before stabilizing, with the fracture angle having a significant influence on the proportion of tensile failure. Lastly, as the fracture angle and length increase, the number of damaged cohesive elements shows an upward trend. This study provides novel perspectives on the tensile behavior of fractured rock masses through the FEM-CZM approach, contributing to a fundamental understanding of the strength characteristics and crack initiation mechanism of rocks under tensile loading conditions. Full article

Granite is widely regarded as an ideal material for the construction of underground liquefied natural gas (LNG) storage reservoirs due to its high mechanical strength and broad geological availability. However, the ultra-low storage temperature of LNG (−162 °C) poses potential risks in altering the permeability of granite, which may compromise the long-term safety and integrity of the reservoir. To investigate the permeability characteristics and microstructural degradation of granite under low-temperature conditions, both coarse-grained and fine-grained granite samples were subjected to a series of experiments, including one-dimensional (1D) gas permeability tests (conducted before and after freeze–thaw cycles ranging from −20 °C to −120 °C), nuclear magnetic resonance (NMR) tests, and two-dimensional (2D) gas permeability tests performed under real-time low-temperature conditions. Experimental results indicated that the gas permeability of granite under real-time low-temperature conditions exhibited a linear increase as the temperature decreased. In contrast, the gas permeability after freeze–thaw cycling followed a nonlinear trend: it increased initially, plateaued, and then increased again as the freezing temperature continued to drop. A further analysis of pore structure evolution and permeability changes revealed distinct degradation mechanisms depending on grain size. In coarse-grained granite, freeze–thaw damage was primarily characterized by the initiation and propagation of new microcracks, which originated as micropores and expanded into mesopores. In fine-grained granite, the damage primarily resulted from the progressive widening of existing fissures, with micropores gradually evolving into mesopores over successive cycles. The study’s findings provide a useful theoretical foundation for the secure subterranean storage of LNG. Full article

With the intensifying global energy crisis, ensuring robust and reliable energy reserves has become crucial, and underground energy storage offers a safe, large-scale, and cost-effective solution. Among various options, salt cavern gas storage is recognized for its excellent sealing capacity and geological stability; however, many natural salt domes contain inherent fissures and interlayers (e.g., gypsum) that can jeopardize operational safety. Hence, this study aims to clarify how different fissure angles and bedding plane dip angles affect the mechanical behavior of salt–gypsum composites, providing insights for enhancing safety measures in underground gas storage facilities. Based on practical engineering demands, we employ finite element software (RFPA2.0) under a confining pressure of 25 MPa to investigate the compressive strength, fractur patterns, and acoustic emission responses of salt–gypsum composites with varying bedding plane and fissure angles. The results indicate that (1) the composite’s compressive strength gradually increases with the fissure angle, being lowest at 0° and highest at 90°; (2) as the bedding plane angle increases, the compressive strength first rises, then decreases, and finally rises again, with its minimum at 60° and maximum at 90°; and (3) when the bedding plane angle exceeds 60°, cracks preferentially develop along the bedding plane, dominating the overall fracture process. These findings provide theoretical guidance for optimizing the design and ensuring the long-term safety and stability of underground salt cavern gas storage systems. Full article

This study investigated the deformation characteristics and mechanisms of the Baiyansizu landslide under the coupled effects of crack development, rainfall infiltration, and road loading. Numerical simulations were performed using GeoStudio software (Version 2018; Seequent, 2018) to analyze geological factors and external disturbances affecting landslide deformation and seepage dynamics. Four additional landslides (Tanjiawan, Bazimen, Tudiling, and Chengnan) were selected as comparative cases to investigate differences in deformation characteristics and mechanisms across these cases. The results demonstrate that rear-edge deformation of the Baiyansizu landslide was predominantly governed by rainfall patterns, with effective rainfall exhibiting a dual regulatory mechanism: long-term rainfall reduced shear strength through sustained infiltration-induced progressive creep, whereas short-term rainstorms generated step-like deformation via transient pore water pressure amplification. GeoStudio simulations further revealed multi-physics coupling mechanisms and nonlinear stability evolution controls. These findings highlight that rear-edge fissures substantially amplify rainfall infiltration efficiency, thereby establishing these features as the predominant deformation determinant. Road loading was observed to accelerate shallow landslide deformation, with stability coefficient threshold values triggering accelerated creep phases when thresholds were exceeded. Through comparative analysis of five typical landslide cases, it was demonstrated that interactions between geological factors and external disturbances resulted in distinct deformation characteristics and mechanisms. Variations in landslide thickness, crack evolution, road loading magnitudes, and rainfall infiltration characteristics were identified as critical factors influencing deformation patterns. This research provides significant empirical insights and theoretical frameworks for landslide monitoring and early warning system development. Full article

Grouting reinforcement is a pivotal approach to enhancing the integrity and load-bearing capacity of fractures in surrounding rock. In this study, standard limestone specimens were fractured through uniaxial compression. Then, the specimens were reinforced with grouting, using ultrafine cement paste containing varying mass fractions of enhancers and a grouting apparatus developed by the authors. After the specimens were cured under standard conditions for 28 days, CT scanning technology was used to investigate the microstructure and grouting effect characteristics of grouted bodies containing different mass fractions of enhancers from a mesoscopic perspective. Then, uniaxial compression tests were conducted on those grouted specimens. The experimental results revealed that the content of the enhancer significantly affected the post-peak characteristics, mechanical parameters, and failure modes of the grouted specimens. When the content of the enhancer increased from 2.50 wt.% to 15.00 wt.%, the uniaxial compressive strength of the grouted specimens exhibited a positive correlation with the enhancer content, with the maximum improvement rate reaching 18.10% compared to the residual strength. However, when the enhancer content ranged from 15.00 wt.% to 20.00 wt.%, the uniaxial compressive strength was negatively correlated with the enhancer content. At an enhancer content of 15.00 wt.%, the overall stability of the grouted specimens was optimal, with all mechanical parameters reaching their maximum values. Utilizing three-dimensional CT scanning and reconstruction technology, it was observed that when the enhancer content was less than 15.00 wt.%, the cracks were concentrated in the limestone matrix rather than in the grouted solid in the edge regions of grouted specimens. However, in the whole specimens, the cracks in the grouted solid exceeded that in the limestone matrix. Conversely, when the enhancer content was greater than 17.50 wt.%, the grouted solid was predominantly distributed within the edge fissures of the specimens, while the internal regions exhibited a lower volume fraction of the grouted solid. In this scenario, the volume fraction of the grouted solid in the specimens was significantly lower than that of the fissures. Full article

This study focuses on researching the influence of Mo on the corrosion mechanism of 1%Ni weathering steel (WS) in simulated marine atmospheric conditions. The Mo element is involved in the reaction, and after hydrolysis, MoO₂ and MoO₃ are produced. The deposition of MoO₂ and MoO₃ occurs in cracks and fissures, rendering the rust layer more uniform and homogeneous. It also furnishes nucleation sites for amorphous oxyhydroxide, which in turn promotes the interweaving of a nanosized oxyhydroxide network. As a result, the rust layer develops into a physical barrier that acquires a protective capacity. Only some of the Mo ions migrate to the vicinity of the Ni element for the hydrolysis reaction, which leads to the difficulty in improving the corrosion rate of the steel with a high Mo content. Hence, the content of Mo element can be controlled within 0.3–0.5% for the 1Ni WS. Full article

The Shitangwan earth fissure is a resultant geological hazard due to prolonged groundwater depletion and land subsidence in Wuxi, China, since the 1980s. Initially observed in 1991, the earth fissure experienced continuous development over the next several decades. Employing a diverse array of techniques, including field monitoring via multilayered borehole extensometers, earth fissure monitoring for lateral and vertical movements, advanced geophysical exploration, and conventional geological investigations, this study aims to mitigate the risks associated with land subsidence and earth fissures. It is found that the groundwater has recovered to the levels in the 1980s, land subsidence and earth fissuring have ceased, and the earth fissuring is closely linked to the land subsidence. A bedrock ridge and a river course are underlying porous Quaternary sediments beneath the earth fissure. The formation of the earth fissure is the result of a combination of factors, including spatial and temporal variations in strata compression, rugged bedrock terrain, and the heterogeneity of the strata profile. Land subsidence is primarily attributed to the deep pumping aquifer and its adjacent aquitards, which are responsive to groundwater recovery with a time lag of a decade, and the land rebound accounts for 2% of the accumulated land subsidence. Estimations suggest that the depth of the earth fissure may have reached the bedrock ridge. The mechanism of the earth fissuring is the coupled effect of tension from the rotation of shallow soil strata along the bedrock ridge and shearing of strata driven by the differential compression of deep strata below the ridge level. Full article

Anchored surrounding rock is prone to large nonlinear deformation and instability failure under dynamic disturbances. The fissures and defects within the surrounding rock make the rock mass’s bearing characteristics and deformation instability behavior increasingly complex. To investigate the effect of the fissure angle on the dynamic mechanical response of the anchored body, a dynamic loading model of the anchored, fissured surrounding rock unit body was established based on the finite difference–discrete element coupling method. The main conclusions are as follows: Compared to the indoor test results, this numerical model can accurately simulate the dynamic response characteristics of the unit body. As the fissure angle increased, the dynamic strength, failure strain, and dynamic elastic modulus of the specimen generally decreased and then increased, with a critical angle at approximately 45°. Compared to 0°, when the fissure angle was 45°, the dynamic strength, failure strain, and dynamic elastic modulus decreased by 17.08%, 15.48%, and 9.11%, respectively. Additionally, the evolution process of cracks and fragments shows that the larger the fissure angle, the more likely cracks are to develop along the initial fissure direction, which then triggers the formation of tensile cracks in other regions. Increasing the fissure angle causes the specimen to rupture earlier, making the main rupture plane more directional. Full article