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26 Results Found

  • Review
  • Open Access
40 Citations
8,511 Views
10 Pages

Horse Welfare During Equine Chorionic Gonadotropin (eCG) Production

  • Xavier Manteca Vilanova,
  • Nancy De Briyne,
  • Bonnie Beaver and
  • Patricia V. Turner

1 December 2019

Collection of blood from pregnant mares for extraction of equine chorionic gonadotropin (eCG) is a critical but relatively unknown and poorly regulated practice in the countries in which it occurs. Equine chorionic gonadotropin is a hormone that is w...

  • Article
  • Open Access
5 Citations
3,340 Views
16 Pages

Production of Mare Chorionic Girdle Organoids That Secrete Equine Chorionic Gonadotropin

  • Riley E. Thompson,
  • Mindy A. Meyers,
  • Jennifer Palmer,
  • D. N. Rao Veeramachaneni,
  • Christianne Magee,
  • Amanda M. de Mestre,
  • Douglas F. Antczak and
  • Fiona K. Hollinshead

The equine chorionic girdle is comprised of specialized invasive trophoblast cells that begin formation approximately 25 days after ovulation (day 0) and invade the endometrium to become endometrial cups. These specialized trophoblast cells transitio...

  • Article
  • Open Access
11 Citations
4,569 Views
22 Pages

Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development

  • Chiara Di Berardino,
  • Alessia Peserico,
  • Giulia Capacchietti,
  • Martina Crociati,
  • Maurizio Monaci,
  • Umberto Tosi,
  • Annunziata Mauro,
  • Valentina Russo,
  • Nicola Bernabò and
  • Barbara Barboni

17 November 2021

The use of assisted reproductive technologies (ART) still requires strategies through which to maximize individual fertility chances. In vitro folliculogenesis (ivF) may represent a valid option to convey the large source of immature oocytes in ART....

  • Article
  • Open Access
1 Citations
2,250 Views
20 Pages

Enhanced Production and Functional Characterization of Recombinant Equine Chorionic Gonadotropin (rec-eCG) in CHO-DG44 Cells

  • Munkhzaya Byambaragchaa,
  • Sei Hyen Park,
  • Myung-Hum Park,
  • Myung-Hwa Kang and
  • Kwan-Sik Min

14 February 2025

Equine chorionic gonadotropin (eCG) hormone, comprising highly glycosylated α- and β-subunits, elicits responses similar to follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in non-equid species. This study aimed to establish...

  • Article
  • Open Access
7 Citations
9,578 Views
12 Pages

Successful Laparoscopic Oviductal Artificial Insemination in the Endangered Tsushima Leopard Cat (Prionailurus bengalensis euptilurus)

  • Akinori Azumano,
  • Miya Ueda,
  • Mika Nomura,
  • Masashi Usui,
  • Midori Ichinose,
  • Yojiro Yanagawa,
  • Satoshi Kusuda,
  • Yuki Matsumoto and
  • Koichi Murata

19 March 2022

The Tsushima leopard cat (Prionailurus bengalensis euptilurus) is an endangered wild felid that lives solely on Tsushima Island, Nagasaki, Japan. Japan’s Ministry of the Environment is working with the Japanese Association of Zoo and Aquariums...

  • Article
  • Open Access
2 Citations
3,124 Views
7 Pages

Combining Fixed-Time Insemination and Improved Catheter Design in an Effort to Improve Swine Reproduction Efficiency

  • Matthew McBride,
  • Rocio Amezcua,
  • Glen Cassar,
  • Terri O’Sullivan and
  • Robert Friendship

29 September 2019

Conventional practice is to breed sows by artificial insemination (AI) at least twice using approximately three billion sperm per insemination upon estrus at standing heat. This research explored the use of combined technologies, including fixed-time...

  • Article
  • Open Access
1,751 Views
15 Pages

Effect of eCG on Terminal Follicular Growth and Corpus Luteum Development and Blood Perfusion in Estrous-Synchronized White Lamphun Cattle

  • Molarat Sangkate,
  • Punnawut Yama,
  • Atsawadet Suriard,
  • Wichayaporn Butmata,
  • Setthawut Thammakhantha,
  • Noppanit Daoloy,
  • Anukul Taweechaipaisankul,
  • Chih-Jen Lin,
  • Pin-Chi Tang and
  • Tossapol Moonmanee
  • + 1 author

18 March 2025

Equine chorionic gonadotropin (eCG) has been shown to improve reproductive performance in cattle by stimulating terminal follicular growth. This study was designed to assess the effects of 400 IU of eCG on TFG and its consequences for luteal developm...

  • Commentary
  • Open Access
7 Citations
7,436 Views
11 Pages

Recommendations for Ensuring Good Welfare of Horses Used for Industrial Blood, Serum, or Urine Production

  • Xavier Manteca Vilanova,
  • Bonnie Beaver,
  • Mette Uldahl and
  • Patricia V. Turner

20 May 2021

Various pharmaceutical products have been derived from horse blood and urine for over a century. Production of biologics and therapeutics from these samples is a niche industry and often occurs in regions with little regulation or veterinary oversigh...

  • Article
  • Open Access
1,205 Views
14 Pages

Effects of Interleukin-6 (IL-6) on In Vitro Cultured Equine Chorionic Girdle Cells

  • Siqin Mu,
  • Yingchao Shen,
  • Hong Ren,
  • Tseweendolmaa Ulaangerel,
  • Minna Yi,
  • Bilig Zhao,
  • Asiya Hao,
  • Qi Liu,
  • Xin Wen and
  • Manglai Dugarjaviin
  • + 1 author

6 February 2025

Introduction: Equine chorionic girdle cells can give rise to the endometrial cup, a structure that secretes equine chorionic gonadotropin (eCG). To date, the mechanisms underlying the proliferation, differentiation, invasion, and hormone secretion of...

  • Article
  • Open Access
5 Citations
2,856 Views
10 Pages

29 September 2022

The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus&ndas...

  • Article
  • Open Access
3 Citations
2,292 Views
10 Pages

Efficacy of Intravaginal Lactic Acid Bacteria, Cell-Free Supernatant, or Enrofloxacin on Vaginitis and Fertility in Ewes Synchronized with Progesterone-Based Protocol

  • Baris Guner,
  • Aslihan Ayalp Erkan,
  • Buse Ozturk,
  • Tevhide Elif Guner,
  • Ihsan Kisadere,
  • Serpil Kahya Demirbilek,
  • Abdulkadir Keskin and
  • Zigo František

An intravaginal sponge impregnated with progesterone is commonly used for estrus induction and synchronization in ewes. Although using an intravaginal sponge containing progesterone positively affects the synchronization rate, varying degrees of vagi...

  • Article
  • Open Access
2 Citations
1,611 Views
15 Pages

Improving Milk Yield, Milk Quality, and Follicular Functionality Behavior in Dairy Cows from the Implementation of Microencapsulated Chili Pepper Supplements in Their Diets

  • Mónica Madrigal-Valverde,
  • Marcus Vinicius Galvão Loiola,
  • José E. de Freitas Júnior,
  • Murilo R. Santiago,
  • Lara Lôbo Dantas,
  • Artur Azevedo Menezes,
  • Isabella de Matos Brandão Carneiro,
  • Gleice Mendes Xavier,
  • Endrigo Adonis Braga Araujo and
  • Juliana Reolon Pereira
  • + 1 author

15 August 2024

The present study evaluates the effect of including microencapsulated hot chili pepper (MHCP) in the diet of crossbred dairy cows on the volume and quality of milk and on ovarian morphofunctionality. Twenty-four crossbred females in their lactating p...

  • Article
  • Open Access
17 Citations
6,525 Views
11 Pages

First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer

  • Adrienne E. Crosier,
  • Julie Lamy,
  • Priya Bapodra,
  • Suzi Rapp,
  • Morgan Maly,
  • Randy Junge,
  • Holly Haefele,
  • Jason Ahistus,
  • Jenny Santiestevan and
  • Pierre Comizzoli

5 October 2020

Approximately 30% of the Association of Zoos and Aquariums cheetah population (~350 total animals) is unlikely to breed naturally due to advanced age, health, or behavioral issues. Aging cheetah females (≥9 y old) are unlikely to become pregnant v...

  • Article
  • Open Access
1 Citations
1,465 Views
13 Pages

Flaws in Estrus Synchronization Protocols Increase Vaginal Prolapse and Hydrometra Risk in Sheep

  • Nikolaos Tsekouras,
  • Ioannis Tsakmakidis,
  • Dimitrios Gougoulis,
  • Mathis A. B. Christodoulopoulos,
  • Christos Kousoulis,
  • Georgios I. Papakonstantinou,
  • Vasileios G. Papatsiros and
  • Georgios Christodoulopoulos

17 May 2025

This study examines the reproductive outcomes of Lacaune-crossbred ewes and hoggets in intensive production systems, focusing on vaginal prolapse and hydrometra associated with flaws in estrus synchronization (E.S.) protocols. Data from multiple farm...

  • Article
  • Open Access
8 Citations
4,155 Views
7 Pages

Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation

  • Lima Rodrigues,
  • Rocio Amezcua,
  • Glen Cassar,
  • Terri L O’Sullivan and
  • Robert Friendship

14 February 2020

In order to efficiently have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows, it is necessary to manipulate the timing of estrus and possibly the timing of ovulation of gilts. Estrus can be synchro...

  • Article
  • Open Access
5 Citations
2,433 Views
15 Pages

Production of Recombinant Single-Chain Eel Luteinizing Hormone and Follicle-Stimulating Hormone Analogs in Chinese Hamster Ovary Suspension Cell Culture

  • Munkhzaya Byambaragchaa,
  • Sang-Gwon Kim,
  • Sei Hyun Park,
  • Min Gyu Shin,
  • Shin-Kwon Kim,
  • Myung-Hwa Kang and
  • Kwan-Sik Min

We produced rec-single chain eel luteinizing (rec-eel LH) and follicle-stimulating (rec- eel FSH) hormones displaying high biological activity in Chinese hamster ovary suspension (CHO-S) cells. We constructed several mutants, in which a linker, inclu...

  • Article
  • Open Access
1,120 Views
14 Pages

Characterization of Trileucine Motif in the C-Terminus of the Equine Lutropin/Choriogonadotropin Receptor

  • Sang-Gwon Kim,
  • Munkhzaya Byambaragchaa,
  • Sei Hyen Park,
  • Ha-Rin Jeong,
  • Jae-Hyek Park,
  • Myung-Hum Park,
  • Myung-Hwa Kang and
  • Kwan-Sik Min

18 November 2024

The lutropin/chorionic gonadotropin receptor (LH/CGR) belongs to the G protein-coupled receptor family, characterized by conserved leucine residues in their carboxyl-terminal cytoplasmic tails. This study aimed to investigate the functional significa...

  • Article
  • Open Access
2 Citations
3,660 Views
15 Pages

Comparison of Gonadotropin-Releasing Hormone versus Estrogen-Based Fixed-Time Artificial Insemination Protocols in Grazing Bos taurus Suckled Beef Cows

  • Luis B. Ferré,
  • Julian Jaeschke,
  • Juliana Gatti,
  • Gerardo Baladón,
  • Ezequiel Bellocq,
  • Gustavo Fernández,
  • Ramiro Rearte,
  • Michael E. Kjelland,
  • Marcos G. Colazo and
  • Jordan M. Thomas

4 September 2023

Fixed-timed artificial insemination (FTAI) protocols for beef cattle in South America are primarily based on estradiol esters and intravaginal progesterone-releasing devices (IVPD). The objective of this study was to determine the optimal gonadotropi...

  • Article
  • Open Access
1 Citations
2,498 Views
15 Pages

24 January 2025

To improve the efficiency of prostaglandin F2α (PG)-based estrus synchronization in sheep, this study assessed the effect of the gonadotropin-releasing hormone (GnRH) included in the long-interval PG treatment regimen for sheep estrus synchroni...

  • Article
  • Open Access
3 Citations
2,565 Views
18 Pages

Stable Production of a Recombinant Single-Chain Eel Follicle-Stimulating Hormone Analog in CHO DG44 Cells

  • Munkhzaya Byambaragchaa,
  • Sei Hyen Park,
  • Sang-Gwon Kim,
  • Min Gyu Shin,
  • Shin-Kwon Kim,
  • Myung-Hum Park,
  • Myung-Hwa Kang and
  • Kwan-Sik Min

This study aimed to produce single-chain recombinant Anguillid eel follicle-stimulating hormone (rec-eel FSH) analogs with high activity in Cricetulus griseus ovary DG44 (CHO DG44) cells. We recently reported that an O-linked glycosylated carboxyl-te...

  • Article
  • Open Access
1 Citations
2,130 Views
15 Pages

Stable Production of a Tethered Recombinant Eel Luteinizing Hormone Analog with High Potency in CHO DG44 Cells

  • Munkhzaya Byambaragchaa,
  • Sei Hyen Park,
  • Sang-Gwon Kim,
  • Min Gyu Shin,
  • Shin-Kwon Kim,
  • Sung-Pyo Hur,
  • Myung-Hum Park,
  • Myung-Hwa Kang and
  • Kwan-Sik Min

We produced a recombinant eel luteinizing hormone (rec-eel LH) analog with high potency in Chinese hamster ovary DG44 (CHO DG44) cells. The tethered eel LH mutant (LH-M), which had a linker comprising the equine chorionic gonadotropin (eLH/CG) β...

  • Communication
  • Open Access
4 Citations
2,907 Views
9 Pages

The Effectiveness of Pharmacological Synchronization of the Estrous Cycle in Hinds (Cervus elaphus L.): A Pilot Field Trial

  • Anna J. Korzekwa,
  • Władysław Kordan,
  • Angelika M. Kotlarczyk and
  • Roland Kozdrowski

19 November 2020

The aim was to estimate the effective pharmacological method of the estrous cycle synchronization by checking the effects of synchronization by measurement of progesterone (P4) and 17-beta estradiol (E2) concentration by RIA and artificial inseminati...

  • Article
  • Open Access
1 Citations
1,507 Views
24 Pages

Seasonal reproduction in sheep reduces reproductive efficiency. Melatonin (MT) plays a crucial role in reproductive processes. The purpose of this study was to assess the effects of a 5-day MT implant pretreatment on estrus synchronization and reprod...

  • Article
  • Open Access
1 Citations
1,799 Views
13 Pages

Selenium + Vitamin E Supplementation in Anestrus Goats: A Strategy to Enhance Reproductive Outcomes Under a Semi-Arid Production System

  • Raquel Santos-Silva,
  • Cesar A. Meza-Herrera,
  • Brenda Castro-Roque,
  • Guadalupe Calderón-Leyva,
  • Cayetano Navarrete-Molina,
  • Oscar Angel-García,
  • Jessica M. Flores-Salas,
  • Angeles De Santiago-Miramontes,
  • Fernando Arellano-Rodriguez and
  • Cesar A. Rosales-Nieto

14 May 2025

Goats are primarily raised in marginal, arid, and semi-arid production systems and exhibit a seasonal reproductive pattern. Extending their reproductive season would enhance food security and improve the economic viability of goat production. Therefo...

  • Article
  • Open Access
1 Citations
1,614 Views
14 Pages

The Effect of By-Pass Linseed Oil Supplementation on the Maternal Antioxidant System during the Embryo-Maternal Recognition Period in Ewes

  • Ignacio Contreras-Solís,
  • Valeria Pasciu,
  • Cristian Porcu,
  • Francesca D. Sotgiu,
  • Neda Todorova,
  • Elena Baralla,
  • Laura Mara,
  • Marilia Gallus,
  • Andrea Cabiddu and
  • Maria Dattena
  • + 2 authors

9 August 2023

This study analyzed the effects of dietary supplementation with by-pass linseed oil (LO; rich in α-linolenic acid) on maternal antioxidant systems at Days 14 and 16 of pregnancy in Sarda ewes. This trial used sixteen dry ewes. Eight ewes (CT gr...

  • Article
  • Open Access
9 Citations
2,662 Views
13 Pages

Effect of Strategic Supplementation of Dietary By-Pass Linseed Oil on Fertility and Milk Quality in Sarda Ewes

  • Ignacio Contreras-Solís,
  • Cristian Porcu,
  • Francesca D. Sotgiu,
  • Fabrizio Chessa,
  • Valeria Pasciu,
  • Maria Dattena,
  • Marco Caredda,
  • José Alfonso Abecia,
  • Giovanni Molle and
  • Fiammetta Berlinguer

13 January 2023

The aim of the present study was to assess whether the strategic supplementation of bypass LO can enhance reproductive indexes—fertility, lambing rate, and prolificacy—in dairy Sarda ewes at the end of lactation. To assess whether LO supp...