Search Results (563)

Background/Objectives: Humans and other vertebrates contain two estrogen receptors (ERs), ERa and ERb. Among cartilaginous fish (sharks, rays, skates), which are chondrichthyans that evolved about 425 million years ago, only activation by steroids of ERb orthologs has been characterized. To remedy this gap in understanding estrogen signaling in chondrichthyans, we studied estrogen activation of orthologs of human ERa and ERb from elephant shark (Callorhynchus milii). Methods/Results: Unexpectedly, we found that C. milii contained three estrogen-responsive ERa genes: ERa1 (596 amino acids), ERa2 (600 amino acids), and ERa3 (599 amino acids) with strong sequence similarity to each other. We also found an estrogen-unresponsive gene, ERa4 (561 amino acids), with a 39 amino acid deletion in the DNA-binding domain. An estrogen-responsive ERb ortholog (580 amino acids) was also present in C. milii. The three active C. milii ERas are of similar length to human ERa (595 amino acids); however, C. milii ERb is longer than human ERb (530 amino acids). We studied transcriptional activation of ERa and ERb by estradiol (E2), the main reproductive estrogen in humans. We also studied estrone (E1), the main postmenopausal estrogen, and estriol (E3), which is synthesized during pregnancy. We determined the half-maximal response (EC50) and fold-activation to E2, E1, and E3 of C. milii ERa1, ERa2, ERa3, and ERb. Among these estrogens, E2 had the lowest EC50 for all four ERs. Fold-activation by E2 and E3 was similar for ERa1, ERa2, ERa3, and ERb. Conclusions: Overall, estrogen activation of C. milii ERa and ERb was similar to that for human ERa and ERb, indicating substantial conservation of the vertebrate ER during the 425 million years since the divergence of cartilaginous fish and humans from a common ancestor. Full article

In areas with frequent wildlife activity, coordinating biodiversity conservation with agricultural production is a critical issue for achieving agricultural sustainability. This study uses farm household survey data collected in 2022 from Asian elephant distribution areas in Yunnan Province, China. It systematically evaluates the effects of Human–Elephant Conflict (HEC) and the wildlife damage compensation policy on farm households’ farmland use behavior. Focusing on farmland adjustment behavior under the context of biodiversity conservation, we develop an analytical framework of “HEC–policy intervention–farm household farmland use behavior.” Using survey data from 1276 farm households, we examine the effects of HEC on farmland transfer-out and farmland abandonment. We also analyze the moderating role of the wildlife damage compensation policy. In addition, we explore the heterogeneity between areas inside and outside nature reserves. The results show that: (1) HEC significantly increase the likelihood of farmland transfer-out and farmland abandonment among farm households; (2) the wildlife damage compensation policy partially mitigates the positive effects of HEC on farmland transfer-out and farmland abandonment; and (3) the effects of HEC on farmland transfer-out and farmland abandonment are more pronounced for farm households outside nature reserves. The wildlife damage compensation policy shows a stronger inhibitory effect on farmland transfer-out inside nature reserves. In contrast, it has a stronger inhibitory effect on farmland abandonment outside nature reserves. From the perspective of farmland use, this study reveals how HEC and policy intervention influence farm households’ farmland allocation behavior. It also provides empirical evidence for improving wildlife damage compensation mechanisms. In addition, the findings help promote synergy between agricultural sustainability and biodiversity conservation. Full article

The world is experiencing incredible biodiversity loss, including the decline of iconic species, such as elephants. The species faces an uncertain future due to habitat loss, human-elephant conflict, poaching and climate change, reminding us of the urgency of acting on a local and global scale. Art has historically been a powerful medium for expressing ideas and emotions, fostering deep connections for people. Therefore, this paper explores the impact of the sharing of scientific content through dance on conservation values in young people. Understanding conservation needs and analyzing what drives people to gain an emotional affinity towards the environment has shown the potential to support and innovate traditional education. The work presented here uses a dance piece, performed through a choreographic process with dance students, to educate an audience about the importance and behavior of the African savannah elephant and the threats to its survival. Our findings indicated differences between the level of knowledge and opinion of the audience throughout the different phases of the methodology explored here, revealing that dance (and artistic) education can provide knowledge and stimulate more empathy for species conservation. Full article

Aortic arch diseases represent one of the most complex domains in cardiovascular surgery due to the need for cerebral protection, anatomical precision, and durable reconstruction. Their management requires highly individualized strategies that balance cerebral protection, systemic organ perfusion, anatomical complexity, and long-term durability. Over recent decades, antegrade cerebral perfusion has significantly reduced neurological morbidity. In parallel, vascular prostheses have progressed from conventional Dacron grafts to hybrid stent graft systems simplifying arch reconstruction and expanded treatment options in high-risk cohorts. Total endovascular arch repair using branched or fenestrated devices has emerged for selected high-risk patients; however, current data remain limited, with early stroke rates of approximately 5–8% and restricted long-term durability evidence. From both clinical and economic perspectives, open and hybrid approaches remain central to durable arch management. Full article

The growing demand for functional and value-added foods has prompted interest in integrating nutrient-rich ingredients and novel encapsulated systems into traditional pasta products. This study aimed to develop and optimize a ravioli dough formulated with elephant foot yam flour (EFYF), wheat flour (WF) and amaranth flour (AF) using mixture design in response surface methodology and to create an innovative filling using encapsulated edible pearls produced from okra mucilage and moringa leaf powder through ionotropic gelation. The pearls and ravioli dough were analyzed for physicochemical, textural, color and nutritional characteristics. Cooked ravioli was investigated for cooking quality and sensory attributes. The optimized dough formulation (46.67 g EFYF, 43.32 g WF, 10 g AF) exhibited desirable hardness (4.64 ± 0.28 N), chewiness (0.40 ± 0.02 N), nutritional, physicochemical and color attributes. The edible pearls demonstrated moderate moisture content (21.18 ± 0.26%), high protein (26.25 ± 0.02%), crude fiber (2.60 ± 0.01%), dietary fiber (8.60 ± 0.52%), high ash content (14 ± 0.62%) and soft gel-like texture. The cooked ravioli showed a cooking time of 8 ± 1 min, high water absorption capacity (209.9 ± 0.34%), minimal solid loss (1.30 ± 0.21%) and favorable sensory scores across appearance, taste, texture and overall acceptability. The study concludes that incorporating encapsulated pearls and nutrient-dense flours can produce a functional, nutritionally enriched ravioli with good technological performance and consumer appeal. Full article

The illegal wildlife trade remains a significant threat to biodiversity in South Africa. The poaching of native species in the country has increased over the years, primarily driven by the demand for abalone, rhino horns, and pangolin scales. This study analysed TRAFFIC wildlife crime records between 1984 and 2025 to identify hotspots, trends in enforcement over time, and the most affected species. We found that provinces such as Gauteng and KwaZulu-Natal have the highest diversity of species affected, while the Western Cape recorded the highest number of incidents, predominantly seizures. Seizure was the most common wildlife activity, followed by poaching and illegal harvesting, with fewer cases of smuggling, breeding, and prosecution. A total of 50 species across nine animal classes were impacted, with white rhinoceros (Ceratotherium simum (Burchell, 1817)), abalone (Haliotis midae (Linnaeus, 1758)), lion (Panthera leo (Linnaeus, 1758)), and ground pangolin (Smutsia temminckii (Smuts, 1832)) among the most frequently targeted. Correlation analysis revealed a strong positive relationship between seizures and arrests (Pearson’s r = 0.90, p = 0.001) across provinces. This indicates a substantial strengthening of law-enforcement activity across provinces, likely driven by enhanced detection or reporting, as reflected in a rising proportion of cases resulting in arrests. Species such as elephants and pangolins were associated with enforcement outcomes, particularly those involving horns, tusks, scales, and dead specimens. There is a need for targeted interventions in high-risk areas, and provinces must collaborate in combating the wildlife trade. Limitations in data completeness and species representation suggest the need for improved surveillance and reporting mechanisms to fully understand and combat illegal wildlife trade in South Africa. Full article

Background: To describe an aortic arch incision and closure technique (AICT) for proximal fixation of a frozen elephant trunk (FET) and to report early outcomes. Methods: We retrospectively reviewed 15 consecutive patients who underwent distal arch repair with an FET using AICT (mean age 77 ± 7 years; 14 men). Indications were distal arch aneurysm (n = 12), acute Stanford type B dissection (n = 2), and distal arch enlargement after thoracic endovascular aortic repair (n = 1). Under circulatory arrest, an oblique arch aortotomy was created, the FET was deployed antegrade, trimmed, and sutured to the native aortic wall during simultaneous closure, allowing extended posterior fixation. Clinical outcomes and postoperative computed tomography were assessed. Results: No ischemic complications related to graft kinking or thrombosis, reoperation for bleeding, stroke, spinal cord ischemia, or organ failure occurred. One patient died of pneumonia on postoperative day 47 (6.7%). Cervical branch reconstruction was required in 12 patients (80%), whereas two patients with type III arch morphology and acute angulation were treated without debranching via a Zone 3 aortotomy. At a median follow-up of 29 months, no proximal endoleak was observed; one distal endoleak occurred without reintervention. Coronary bypass grafts remained patent in all patients with concomitant or prior CABG. Conclusions: AICT provided secure proximal FET fixation and arch closure while preserving the ascending aorta, offering an alternative to total arch replacement in selected distal arch pathologies. Full article

This paper introduces an enhanced load frequency regulation strategy for isolated renewable microgrids, leveraging an Active Disturbance Rejection Control (ADRC) framework optimized through Elephant Herding Optimization (EHO). A detailed microgrid model, encompassing a variety of energy generation and storage units, is implemented in a simulation environment. The effectiveness of the proposed ADRC-EHO method was assessed through comparative analysis with established control techniques: Particle Swarm Optimization (PSO)-tuned ADRC and H∞ control under diverse operational scenarios. These scenarios included deterministic and stochastic load disturbances, as well as variations in microgrid parameters. The findings demonstrate that the ADRC-EHO approach consistently yields superior performance, with improved robustness and a more rapid response to frequency fluctuations. The optimization of ADRC parameters using EHO effectively countered the challenges of intermittent renewable energy integration. Full article

Landscape features often shape maternal genetic structure by influencing connectivity. In this study, habitat fragmentation, a major threat to the Asian elephant (Elephas maximus), was assessed through an integrated approach involving mitochondrial DNA (mtDNA), habitat-suitability modeling, and circuit-based landscape-resistance analyses. Two regions from Thailand, Phu Khieo (PK) and Khao Ang Rue Nai (ARN) Wildlife Sanctuaries, were investigated. Fourteen mtDNA haplotypes were identified among 66 samples, with relatively high diversity and population expansion in PK. Maternal genetic differentiation was identified between the PK and ARN groups. Environmental variables, such as urbanization and road proximity in ARN and topographic wetness and stream distance in PK, were associated with genetic distances, suggesting these features restricted female-mediated connectivity. Fine-scale spatial analysis revealed significant local genetic structure only in PK, whereas no autocorrelation was detected in ARN, suggesting potential impacts of fragmentation. Genetic landscape surfaces illustrated spatial heterogeneity, pinpointing isolation zones near high anthropogenic disturbance. These findings demonstrate that broad-scale models may overlook fine-scale patterns of maternal genetic isolation. Therefore, conservation strategies should incorporate spatially explicit analyses to identify and restore crucial movement corridors, particularly in fragmented regions like ARN, for promoting connectivity and population viability. Full article

Rabies is an acute, progressive, viral encephalitis. Warm-blooded vertebrates are susceptible. Major reservoirs reside in the Chiroptera and Carnivora. Among the latter, representatives include dogs, ferret badgers, foxes, jackals, mongooses, raccoons, and skunks. Within the Carnivora, pinnipeds represent a diverse group of >30 extant species. These marine mammals range from the Arctic to Antarctica, but there is no review about rabies in this group. Apparently, only a single 1980 case of rabies occurred from Svalbard in a ringed seal (Phoca hispida). However, in 2024, incidental cases appeared within South African Cape fur seals (Arctocephalus pusillus). Retrospective testing of archival material identified suspect cases dating back to 2022. Currently, more than 80 cases have been documented in seals. Moreover, a new 2025 focus appeared in Namibia and cases in Angola are predictable. Viral characterization supports spillover infection via rabid black-backed jackals (Lupulella mesomelas). A host shift appears likely, with ongoing seal intraspecific transmission. Given the unique nature of this epizootic, implications for the southern hemisphere abound. Unfortunately, comprehensive data are lacking on pinniped specimens examined outside of southern Africa. For example, although Antarctica is considered ‘rabies-free’, minimal international standards for support are unmet. No routine laboratory-based surveillance occurs. This enzootic rabies focus among seals in southern Africa presents unique challenges for the region and a rare opportunity for considering broader surveillance. Besides targeted parenteral vaccination of fur seals, local engagement involves vagrant species, including elephant (Mirounga leonina) and leopard seals (Hydrurga leptonyx). The void of regional pinniped surveillance, especially encompassing the Southern Ocean would require considerable proactive local resolution and much wider collaboration regarding future concerns to both public health and conservation biology. Full article

Protected national parks continue to face increased pressure from the expansion of human–wildlife interface zones, where habitat encroachment promotes human–wildlife contact and zoonotic disease transmission. Gastrointestinal parasites (GIPs) are a significant health issue in wild Asian elephants (Elephas maximus), affecting their fitness, survival, and potential for cross-species transmission. This study aimed to investigate the prevalence of GIPs among wild elephants at Khao Sip Ha Chan National Park in eastern Thailand. Direct smear, formalin-ethyl acetate sedimentation, flotation, and McMaster egg per gram (EPG) counting were used to examine 135 fecal samples from three populations. The findings showed that nematodes (Strongyle-type and Strongyloides spp.) and trematodes (Paramphistomum spp. and Fascioloides jacksoni) were identified. All samples were positive for at least one parasite species, and 84.4% were affected by mixed infections. The prevalence of Strongyle-type and Strongyloides spp. varied significantly among the studied populations, but Paramphistomum spp. had a moderate prevalence, and Fascioloides jacksoni had a low prevalence. The high parasite burden could be explained by environmental factors, host density, and movement patterns influencing parasite transmission, and these factors should be further investigated. These findings provide crucial baseline data and underscore the need for integrated parasite surveillance, alongside long-term conservation and future research. Full article

Classification techniques, reliant on annotated data for autonomous decision training, have become pivotal tools in diverse domains. These techniques rely on models like Backpropagation Neural Networks (BPNNs). However, BPNNs frequently trap local optima, leading to suboptimal classification accuracy, and its convergence speed is relatively slow, which affects efficiency in complex and non-linear process data classification applications. Existing optimization algorithms struggle to balance global exploration and local exploitation when adjusting BPNNs. Addressing these limitations, this paper proposes a BP classifier based on an Elephant Herding Optimization with Multi-Learning strategy (MLEHO), termed MLEHO-BPC. The proposed MLEHO establishes a triple learning framework. Firstly, a collective learning stage incorporates two different adaptive operators into the original algorithm to strengthen global exploration. Subsequently, a group learning stage is designed, integrating exemplar, deskmate, and random learning methods to enhance convergence efficiency. Finally, a tutorship learning stage, guided by fitness value discrimination, empowers the algorithm to escape local optima. Benchmark function tests confirm MLEHO’s superiority in convergence speed and stability over comparative algorithms. Furthermore, MLEHO replaces traditional gradient descent, reformulating the BPNN’s update mechanism to optimize weights and thresholds. Validated on classification datasets and a Ti6Al4V process classification problem, MLEHO-BPC demonstrates exceptional classification accuracy and robustness against other algorithm classifiers. Full article

Interspecies communication is increasingly recognized as an affective–cognitive process co-created between humans and animals rather than a one-directional transmission of signals. This review integrates findings from ethology, neuroscience, welfare science, behavioral studies, and posthumanist ethics to examine how emotional expression, communicative intentionality, and relational engagement shape understanding across species. Research on primates, dogs, elephants, and marine mammals demonstrates that empathy, consolation, cooperative signaling, and multimodal perception rely on evolutionarily conserved mechanisms, including mirror systems, affective contagion, and oxytocin-mediated bonding. These biological insights intersect with ethical considerations concerning animal agency, methodological responsibility, and the interpretation of non-human communication. Emerging technological tools—bioacoustics, machine vision, and AI-assisted modeling—offer new opportunities to analyze complex vocal and behavioral patterns, yet they require careful contextualization to avoid anthropocentric misclassification. Synthesizing these perspectives, the review proposes a relational framework in which meaning arises through shared emotional engagement, embodied interaction, and ethically grounded interpretation. This approach highlights the importance of welfare-oriented, minimally invasive methodologies and supports a broader shift toward recognizing animals as communicative partners whose emotional lives contribute to scientific knowledge. This review primarily synthesizes empirical and theoretical research on primates and dogs, complemented by selected examples from elephants and marine mammals, which provide the most developed evidence base for the affective–cognitive and relational mechanisms discussed. Full article

Objectives: Thoracic endovascular aortic repair (TEVAR) is rarely indicated on an urgent or emergent basis soon after open surgical repair of type A aortic dissection (TAAD), and systematic data on clinical outcomes are therefore missing. In the present study, we analyze a contemporary case series regarding the outcome after urgent and emergent endovascular treatment of the downstream thoracic aorta, following open surgery for TAAD. Methods: The study was conducted as a retrospective observational analysis. From January 2024 until April 2025, seven patients (four male, aged 56.8 ± 5.6 years) were treated with TEVAR on an urgent or emergent basis within 48 h after open surgical repair of TAAD at our institution. In all seven patients, the initial dissection extended from the ascending to the abdominal aorta. All seven patients had previously received emergent open surgical repair by ascending aortic repair combined with hemiarch replacement (five patients) or total arch replacement, utilizing the frozen elephant trunk (FET) technique (two patients). Results: In four patients, the indication for urgent TEVAR was due to true lumen collapse (TLC) of the downstream aorta with resulting visceral or peripheral malperfusion symptoms. Three patients were treated on an emergent basis, due to rupture of the descending thoracic aorta with a resulting hemorrhage. Technical success of the TEVAR procedure was 100%. Thirty-day mortality was 0% in the TLC cases but 66% in the ruptured cases, where two of three patients died postoperatively due to the consequences of severe hemorrhagic shock. Within the surviving patients, no subsequent aortic events occurred during follow-up. Late mortality was 0%. The follow-up period was 15.7 ± 2.0 months. Conclusions: In our case series, mortality of urgent or emergent TEVAR soon after open surgical repair for TAAD is substantial, especially in patients that were treated due to acute rupture of the descending thoracic aorta and consecutive hemorrhagic shock. On the other hand, true lumen collapse with resulting malperfusion was successfully treated by instant TEVAR application in all patients without late aortic complications by the midterm follow-up. Full article

Understanding how species share resources (niche dynamics) and associate with each other is crucial for maintaining stable ecological communities. Using infrared camera traps, we constructed spatial association networks for an isolated Asian elephant population. The Asian elephant (Elephas maximus), a keystone species in tropical forests, faces significant threats from habitat fragmentation and human disturbances, particularly in the isolated population of Nangunhe National Nature Reserve, Yunnan, China. Using infrared camera trapping, niche analysis, and interspecific association models, we examined the ecological role of Asian elephants and their sympatric species networks in fragmented habitats. We identified 44 species, including 11 species with higher relative abundance showing significant ecological correlations with elephants. Asian elephants exhibited the broadest spatial distribution, consistent with their role as ecological engineers due to high environmental tolerance and diverse resource utilization. Sympatric herbivores exhibited moderate spatial co-occurrence. Wild boars (Sus scrofa), red-bellied squirrels (Callosciurus erythraeus), northern pig-tailed macaques (Macaca leonina), and red junglefowl (Gallus gallus) demonstrated significant spatial associations with elephants. Lambda coefficient analysis revealed asymmetric associations reflecting spatial reliance of red-bellied squirrels and wild boars on elephant activity zones. Temporally, Asian elephants exhibited a stable bimodal activity pattern at dawn and dusk. Despite varying degrees of diel overlap with sympatric species, no significant temporal avoidance was detected, suggesting fine-scale coexistence mechanisms beyond the temporal dimension. We argue that conservation strategies are in urgent need of a transformation from single-species protection to the preservation of ecological interaction networks. This study clarifies the dominant position of Asian elephants in the community by mapping the spatial association networks between Asian elephants and sympatric species, and its findings hold substantial guiding significance for the recovery and protection of isolated Asian elephant populations. Full article