Search Results (2,284)

Transmission of water, vector, and foodborne diseases tends to increase with climate variability, disproportionately affecting vulnerable communities. Globally, this dynamic contributes to adverse health outcomes in low- and middle-income countries such as Mozambique. Nevertheless, the influence of climate variability on disease patterns remains unclear. We assessed the potential relationship between climate variables and diseases, including diarrheal diseases, malaria, and malnutrition, in four districts around the Revuè sub-basin. An ecological, longitudinal, retrospective study conducted from 2016 to 2024 used secondary weekly disease data and annual malnutrition records. We applied a Generalized Additive Model framework to evaluate non-linear associations between climate factors and disease case counts. Our main findings showed that while higher maximum temperature was associated with increased cumulative malaria case counts, cumulative and heavy rainfall (~100 mm/week) was associated with decreased diarrheal disease case counts. Higher prevalence of moderate acute malnutrition is associated with a decrease in annual mean total precipitation (p = 0.0062). In contrast, higher prevalence of severe acute malnutrition is associated with a decrease in annual mean temperature (p = 0.0312). This study contributes to understanding climate variables associated with water, vector, and foodborne diseases, enhancing our understanding of factors contributing to overall food insecurity and affecting public health. Full article

Agricultural practices based on sustainable principles (e.g., conservation tillage, crop rotation, cover cropping, and the application of organic inputs) have been tested for their potential to improve soil structure, enhance soil organic matter, and support agrobiodiversity. These practices are directly linked to soil microbial diversity. Diverse soil microbial communities play multiple roles in promoting beneficial interactions between plants and their environment and in maintaining functional agroecosystems. Key functions enabled by soil microorganisms are carbon dynamics, nutrient cycling, soil structure improvement, pathogen suppression, plant growth promotion, and stress tolerance. Soil microorganisms are increasingly recognized as a promising but still underexploited source in tackling sustainability challenges in agricultural production. However, their potential varies depending on the interactions among abiotic and biotic factors, as well as the applied cultivation practices. In recent decades, advances in DNA extraction from soil and next-generation sequencing (NGS) technologies have enabled comprehensive characterization of microbial diversity, community composition, and functional potential for assessing soil health and agroecosystem functioning. Understanding, predicting, and exploring relevant plant–soil–microbiome interactions are essential for enhancing agroecosystem capacity for sustainable production. This review paper highlights how different factors and agricultural practices affect microbiome biodiversity. The focus is on microbiome approaches that integrate information on community composition with assessments of functional potential and measured microbial activity and ecosystem processes, combining state-of-the-art molecular monitoring, ecological indicators, and predictive modeling to support evidence-based management recommendations, distinguishing approaches that are currently applicable in agricultural practice from those that require further experimental validation. Full article

This study assessed the geographic, demographic, and temporal factors underlying the evolution of the HIV/AIDS epidemic across the health macroregions of Minas Gerais, Brazil, comparing the periods before (2009–2014) and after (2015–2022) the implementation of universal HIV notification and immediate antiretroviral therapy (ART). An ecological study was conducted using statewide surveillance and clinical monitoring databases. Epidemiological indicators, including HIV detection, AIDS incidence, AIDS-related mortality, ART coverage, and sustained viral suppression (SVS), were analyzed according to sex and health macroregion using generalized linear models with negative binomial regression and spatial analysis. HIV detection increased markedly following the expansion of mandatory notification, while ART coverage and SVS also increased significantly. In contrast, AIDS incidence and AIDS-related mortality declined during the later period. Substantial heterogeneity was observed across macroregions, with important differences in epidemiological indicators and HIV care cascade performance. These findings demonstrate that the benefits of expanded HIV detection and universal ART have not been distributed uniformly across the state. Regional disparities in access to diagnosis, treatment, and long-term care remain important barriers to HIV control, highlighting the need for targeted, region-specific public health strategies to reduce territorial inequalities and strengthen the HIV care cascade. Full article

Groundwater is the main source of drinking, irrigation, and ecological water in the Baiyangdian Lake Basin (BLB), but growing human activity has raised concerns about its quality. This study assesses major ion geochemistry and multi-receptor environmental risks across the BLB using 1113 groundwater samples (983 shallow, 130 deep). Risks to drinking water supply, agricultural irrigation, and wetland health are evaluated within a single framework, and Positive Matrix Factorization (PMF) is used for quantitative source apportionment. Shallow groundwater shows markedly higher dissolved solids and nitrate than deep groundwater, with 20.7% of shallow samples exceeding the WHO nitrate guideline. Multi-receptor assessment finds that 41.2% of shallow wells pose risk to at least one endpoint and 12.5% to two or more simultaneously. PMF identifies three sources—geogenic weathering, agricultural nitrate, and wastewater discharge—with nitrate as the largest single contributor to water quality impairment. The findings point to an urgent need for targeted agricultural non-point source control and provide a basis for nested, multi-scale groundwater management in the BLB and similar intensively exploited alluvial aquifers. Full article

The Qinghai–Xizang (Formerly Tibet) Plateau, known as the Roof of the World, is an important ecological security barrier for Asia and globally; its natural environment is fragile and challenging to repair after destruction. Prior to the 21st century, waste management in China primarily relied on unregulated direct dumping. To investigate the environmental impacts of open dumpsites in high-altitude regions, this study focused on a representative dumpsite in the northern Xizang region. The aim was to characterize the occurrence and distribution patterns of heavy metals (HMs) in its surrounding soil, conduct source apportionment, and perform comprehensive risk assessment. The results showed low levels of mercury (Hg) and excessive levels of all other HMs. In addition, all HMs had the highest content in the residual fraction and the lowest content in the weak-acid fraction, which is less bioavailable. The Absolute Principal Component Score–Multiple Linear Regression (APCS-MLR) and Positive Matrix Factorization (PMF) results showed that the dumpsite contributed the most to cadmium (Cd); agricultural sources contributed the most to zinc (Zn); Hg mainly came from atmospheric transport and traffic sources; Pb was mainly derived from traffic and natural sources; and copper (Cu), nickel (Ni), chromium (Cr), and arsenic (As) mainly came from the dumpsite and natural sources. In addition, this research found that this dumpsite poses a relatively high HM risk to human health; oral and respiratory routes are the main non-carcinogenic routes, As and Cr contribute more to the adult and child groups, and As contributes the most to carcinogenic risk. The calculation results of the pollution assessment method indicate that the pollution of the natural environment from this dumpsite is at a low level, and immediate remediation measures are urgently needed to achieve site decontamination. Full article

The Soil Quality Index (SQI) is a vital tool for evaluating soil quality; however, traditional approaches seldom integrate microbial data from high-throughput sequencing—commonly used to characterize soil microbial communities—into the SQI framework. This study enhances the SQI by incorporating microbial indicators derived from high-throughput sequencing, establishing a more comprehensive evaluation system. We collected soil samples from mining areas and analyzed their fundamental physicochemical properties and microbial indicators. Four SQI models were constructed using different indicator sets: (1) only physicochemical properties (T-SQI); (2) physicochemical properties and bacterial α-diversity (α-SQI); (3) physicochemical properties, α-diversity, and relative abundances of the top five abundant bacteria (αMA-SQI); and (4) physicochemical properties, α-diversity, and relative abundances of the top five bacteria based on LDA scores (αBM-SQI). Results demonstrated that integrating multi-level microbial indicators improved the rationality of soil quality rankings and significantly strengthened correlations with α-diversity. Gemmatimonadota was consistently selected in the Minimum Data Set (MDS) for both αMA-SQI and αBM-SQI, highlighting its ecological importance. Statistically, microbial indicators at the order and family levels were most suitable for inclusion in the MDS, as their results deviated least from the total dataset. In conclusion, incorporating microbial diversity across taxonomic levels refines the SQI, enabling a more accurate and holistic assessment of soil health. Full article

Long-term mining activities can cause complex ecological and environmental problems, including mineral surface exposure, dust-related disturbance, and vegetation degradation, while conventional ecological indices may not fully characterize the distinctive ecological conditions of mining areas. This study developed a mining-area Modified Remote Sensing Ecological Index (MRSEI) by incorporating the Lithological Mineral Index (LMI), Normalized Dust Difference Index (NDDI), and Vegetation Health Index (VHI) into the Remote Sensing Ecological Index (RSEI) framework. Object-based analytical units were constructed using Landsat time-series data from 2000 to 2024 and G-means clustering, followed by the development of an Object-Based XGBoost-SHAP (OB-XGBoost-SHAP) framework to quantify the relative model contributions and nonlinear associations of explanatory factors. The results showed that, in the Daye mining area (DMA), MRSEI provided clearer differentiation of ecological quality among land-use types than RSEI and Mining-Specific Eco-Environment Index (MSEEI), with an overall gradient of forest land > cultivated land > construction land > mining land. Annual Principal Component Analysis (Annual-PCA) and Global Principal Component Analysis (Global-PCA) results showed high consistency (Pearson r = 0.985). Across the seven evaluation years, the mean MRSEI of the DMA increased from 0.5493 in 2000 to 0.6035 in 2024, representing an overall change of 9.87%. The object-based model consistently outperformed the regular-grid model under random splitting, while model performance decreased under spatially separated validation but retained moderate predictive capability. Particulate Matter (PM10), Digital Elevation Model (DEM), Temperature (TEM), and Land Use Intensity (LU) showed relatively high model contributions, with their relative importance varying across different periods. This study integrates mining-specific ecological characterization, object-based spatial analysis, and explanatory-factor contribution analysis, providing a methodological reference for ecological quality assessment and long-term change monitoring in mining areas. Full article

Mineral homeostasis in horses is traditionally attributed to the coordinated regulation of the intestine, kidneys, skeleton, and endocrine system. Emerging evidence suggests the gut microbiota also shapes this process by modifying the intestinal environment where mineral absorption occurs. Despite the growing interest in host–microbiota interactions, the mechanisms linking the equine gut microbiota to mineral metabolism remain poorly understood and have not been comprehensively synthesized. This review integrates current knowledge of equine gastrointestinal physiology, gut microbial ecology, and mineral metabolism to examine how the gut microbiota influences mineral homeostasis, focusing on microbial fermentation, bioactive metabolite production, luminal pH regulation, epithelial barrier integrity, modulation of intestinal mineral transporters, and interactions with endocrine and immune pathways. The assessment of macro minerals such as calcium, phosphorus, and magnesium, along with trace minerals like iron, zinc, copper, selenium, and manganese, was conducted with a critical approach. This evaluation differentiates between results derived from equine research and those inferred from studies on other species. However, direct mechanistic evidence in horses remains limited, highlighting the need for future research. Advancing equine microbiome research through integrated microbiome profiling, metabolomics, molecular biology, and mineral balance studies will improve our understanding of host–microbiota interactions and support microbiome-informed nutritional strategies to enhance mineral utilization, gastrointestinal health, skeletal function, and long-term equine health and performance. Full article

The present study assessed potentially toxic elements (PTEs) and microbial contamination in soil and groundwater around a municipal solid waste dumpsite in Zomba, Malawi. The potential ecological and health risks to communities were also examined. The results revealed that wet season groundwater had elevated total coliforms (20,900 CFU/100 mL), Escherichia coli (3300 CFU/100 mL), Staphylococcus aureus (2500 CFU/100 mL), and Vibrio cholerae (5900 CFU/100 mL), which were significantly higher than the permissible limits of the Malawi Standards. In water samples, PTEs, particularly cadmium (Cd; 0.011–0.08 mg/L) and chromium (Cr; 0.02–0.41 mg/L), were detected at concentrations of concern. In the soil samples, lead (Pb) concentrations ranged from 0.16 to 224.05 mg/kg, copper (Cu) concentrations ranged from 3.03 to 94.86 mg/kg, Cd concentrations varied between the BDL and 0.89 mg/kg, arsenic (As) ranged from BDL to 1.88 mg/kg, and the Cr varied between 0.07 and 0.91 mg/kg. Furthermore, the cancer risk (CR) assessment indicated that all sampling points had CR levels greater than 1 × 10⁻³ for adults, with 40% of the sampling points showing elevated CR levels for infants and children, highlighting the cancer risk from Cd exposure, especially among vulnerable populations. Full article

The Berca–Arbănași region of Buzău County (Romania) is of exceptional geochemical interest due to diapiric structures, active mud volcanoes, and historical subsurface hydrocarbon deposits. This study presents the first comprehensive geochemical assessment of surface soils from the Berca–Arbănași Subcarpathian zone, based on the analysis of 27 soil samples collected along three north–south transects and the determination of 12 potentially toxic elements (As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, V, Zn) using aqua regia digestion and ICP-MS. The local geochemical background was calculated using the iterative median ± 2MAD method. Twelve pollution and ecological risk indices were computed: the Pollution Index (PI), Contamination Factor (CF), Geoaccumulation Index (Igeo), Enrichment Factor (EF), Pollution Load Index (PLI), Modified Degree of Contamination (mCd), Nemerow Integrated Pollution Index (PINemerow), Ecological Risk Factor (E_rⁱ), Ecological Risk Index (RI), Mean Effect Range-Median Quotient (MERMQ), Degree of contamination (Cdeg), and the V/Ni petroleum origin indicator. Results show that 66.7% of samples are classified as polluted (PLI ≥ 1; mean = 1.102), with moderate enrichment in Cd, Cu, Hg, Pb, and Zn, attributable to diffuse anthropogenic sources. The ecological risk index (RI) remains low across all samples (mean = 35.96; all < 150), indicating that, despite moderate pollution, ecological risk is currently low. The V/Ni ratio (0.391–0.884, mean = 0.608) is below 1.0 for all samples, indicating a lithogenic (not petroleum) origin of vanadium and nickel and confirming a negligible geochemical impact of mud volcanoes and hydrocarbon extraction activities in the area at the sampled locations. The study establishes baseline geochemical reference values for the Berca–Arbănași area and provides data for sustainable land management, environmental monitoring, and evidence-based policymaking. These results directly support the objectives of the EU Soil Strategy 2030 and align with the United Nations Sustainable Development Goals on food security (SDG 2), good health and well-being (SDG 3), and life on land (SDG 15). Full article

Makkah faces substantial heat-stress challenges associated with extreme temperatures, dense urban form, and the large numbers of pilgrims present during Hajj and Umrah, creating important public health concerns. Recent heat-related fatalities highlight the need for complementary strategies that address outdoor as well as indoor heat exposure, alongside conventional cooling approaches such as air conditioning. This article examines the potential role of nature-based and complementary engineered interventions in mitigating urban heat stress in Makkah, focusing on afforestation, urban greening, and the possible use of artificial water bodies, contingent on sustainable water management. Drawing on case studies and published evidence from arid and heat-prone regions, including China, Pakistan, Saudi Arabia, and the wider Middle East, we summarize reported cooling effects, implementation experience, and feasibility considerations and assess their potential relevance to Makkah. The perspective highlights critical challenges related to water scarcity, spatial constraints, ecological impacts, and governance, while proposing phased implementation pathways that could be evaluated incrementally. If carefully designed and integrated with urban planning and climate-adaptation strategies, nature-based and complementary interventions could potentially reduce human heat stress, reduce cooling demand, and strengthen climate resilience in Makkah. However, their effectiveness, water requirements, environmental impacts, and scalability require evaluation under Makkah-specific environmental and operational conditions. Full article

Ticks are among the most important arthropod vectors affecting human and animal health worldwide. We present a set of globally harmonized indicators integrating hazard, exposure, and vulnerability associated with human-biting ticks and with two major tick-borne pathogens, Borrelia burgdorferi sensu lato (Lyme borreliosis), and Orthonairovirus haemorrhagiae (Crimean–Congo haemorrhagic fever virus, CCHFV). Hazard indices were derived from the distributions of confirmed vector tick species. Exposure was incorporated through rural human population density, livestock density, and the abundance of competent vertebrate reservoirs and/or amplification hosts, as applicable. Vulnerability was assessed using country-level indicators related to healthcare accessibility, socioeconomic development, and demographic dependency. The indices show spatially structured global patterns. Tick hazard is concentrated in eastern North America, Europe, Central and East Asia; Africa displays fragmented but substantial hotspots. The B. burgdorferi s.l. index is restricted to the Northern Hemisphere, following the distribution of Ixodes spp. and associated reservoir communities, with highest exposure in Europe and North America. The CCHFV index is confined to the Old World, extending from the Mediterranean region through the Middle East and Central Asia into Sub-Saharan Africa, reflecting the distribution of Hyalomma ticks and ungulates. The vulnerability indicators highlight regional patterns of high exposure and limited adaptive capacity, particularly in Sub-Saharan Africa and parts of the Middle East. Our framework provides the first global set of harmonized, high-resolution indices integrating vector ecology, reservoir communities, exposure, and vulnerability for major tick-borne diseases of humans. It offers a flexible platform for comparative epidemiology, surveillance prioritization, and public health decision-making at multiple administrative scales. Full article

Seed-associated microorganisms influence seed quality, seedling establishment, and plant health and also constitute a major source of seed-borne pathogens. Cold atmospheric plasma (CAP) has emerged as a promising alternative to chemical seed treatments because of its antimicrobial activity, although its effects on fungal communities associated with developing seedlings remain poorly understood. Here, Arabidopsis thaliana seeds from two ecotypes (Columbia and Landsberg erecta) were exposed to CAP for 5 or 15 min. Seed decontamination efficiency was assessed by culturing on malt extract agar and nephelometric analyses, while fungal communities associated with seedlings derived from treated and untreated seeds were characterized by ITS1 amplicon sequencing. CAP efficiently reduced fungal contamination without affecting seed germination. CAP altered the composition of fungal communities associated with developing seedlings, but the magnitude of these changes depended on seed batch and ecotype. Dominant taxa markedly declined after treatment, whereas several low-abundance taxa increased in relative abundance. These findings demonstrate that CAP is an effective pesticide-free technology for seed decontamination and can also reshape fungal communities associated with developing seedlings, highlighting broader ecological consequences of plasma-based seed treatments. Full article

Background/Objectives: This prospective cross-sectional study quantified veterinary antimicrobial residues in soils from 27 commercial beef feedlots across multiple regions of Argentina and production scales. Methods: Using a validated Ultra-Fast Liquid Chromatography–tandem mass spectrometry (UFLC-MS/MS) method, we measured 15 target antimicrobials in pooled corral soil samples. Results: Monensin was detected in all feedlots and showed the highest detection frequency and widest concentration range; peak concentrations exceeded 15,000 ppb in some sites. Oxytetracycline presented pronounced, site-specific peaks (approaching 14,000 ppb), consistent with its strong adsorption to soil organic matter and limited biodegradation. These results indicate localised hotspots of antimicrobial loading in feedlot environments. These elevated concentrations signify substantial antimicrobial loading in confined animal environments and potential hotspots for environmental selection pressure. Compounds absent from farm treatment records were detected in 40.7% of feedlots, correlating with antimicrobial residues not matching available AMU records, including oxytetracycline, tulathromycin, cefquinome, enrofloxacin, and ciprofloxacin among others. Multiple non-exclusive explanations are plausible (historical persistence, manure redistribution, incomplete records, off-label use, or cross-contamination). The detection of critically important antimicrobials (e.g., fluoroquinolones and advanced cephalosporins) in soils raises One Health concerns about environmental selection pressure and the potential dissemination of resistance determinants. Conclusions: These findings underscore gaps in stewardship and the need for integrated environmental monitoring and improved antimicrobial usage traceability. These data provide a baseline for ecological risk assessment and resistome studies and can inform evidence-based policy and stewardship interventions in Argentine feedlot systems. Full article

Livestock faeces constitute an important environmental interface within the One Health framework, as bacteria shed by animals are directly exposed to external environmental conditions, where they may persist and subsequently disseminate across the human, animal, and environmental sectors. The ability of these microorganisms to tolerate environmental stresses is therefore critical for their persistence and ecological distribution. In this study, the responses of 5 Pseudomonas putida, 15 Pseudomonas fulva, and 12 Alcaligenes faecalis isolates recovered from livestock faeces to different environmental stress conditions were investigated. Bacterial growth was evaluated under different temperatures, increasing NaCl concentrations, and carbon starvation, while biofilm formation was assessed using the microtiter plate assay. Growth was markedly affected by all stress conditions, although substantial variability was observed among isolates of the same species. Despite reduced growth under the most adverse conditions, all three species remained capable of growing across a wide range of environmental stresses. A. faecalis exhibited greater salt tolerance and biofilm-forming capacity than Pseudomonas spp., with biofilm formation detected in almost all isolates of both species. Furthermore, no statistically significant correlations were detected among the evaluated stress tolerance traits. These findings demonstrate that livestock-associated P. putida, P. fulva, and A. faecalis possess physiological characteristics that promote persistence under diverse environmental conditions, highlighting the potential role of livestock faeces as environmental reservoirs of opportunistic bacteria within the One Health context. Full article