Search Results (21)

Fenugreek (Trigonella foenum-graecum L.) is an emerging forage crop known for its high nutritional value and adaptability to diverse environmental conditions, making it a promising alternative in sustainable livestock feeding systems in the Mediterranean region. A field experiment was established at the Agricultural University of Athens during the growing season 2020–2021 in a split-plot design with five fertilization treatments (Biocyclic-Vegan Humus Soil; BHS, Farmyard Manure; FMA, Organic Compost; OCP, Inorganic Fertilizer; IFZ, and No Treatment Control; CTRL, and two main salinity treatments (High Salinity Level; HSL, and Normal Salinity Level; NSL). The Forage Quality Index (FQI) was the highest under BHS at NSL (45) and lowest under CTRL at HSL (32), emphasizing the positive impact of organic fertilization. Crude ash (CA) was higher under NSL (9.7%), with OCP and IFZ performing best, while salinity reduced CA under CTRL. Crude fiber (CF) increased under salinity, particularly with OCP and IFZ, whereas BHS and FMA at NSL showed the lowest CF (15.8%), enhancing digestibility. Total fat (TF) was the highest under BHS and FMA at NSL (5.8%) and lowest under IFZ and CTRL at HSL (4.0%), underscoring the importance of balanced fertilization in maintaining fat content. These results highlight the potential of organic amendments to improve nutrient availability, digestibility, and overall feed value. Full article

Corn stalk fibers extracted from cattle manure (CSFCM) represent a unique class of natural fibers that undergo biological pre-treatment during ruminant digestion. This study systematically investigates the optimization of CSFCM-reinforced friction materials through controlled silane treatment (2–10 wt.%). The biological pre-treatment through ruminant digestion creates distinctive fiber properties that influence subsequent chemical modification. Physical characterization revealed that optimized interface modification at 6 wt.% silane treatment (CSFCM-3) effectively enhanced the fiber–matrix compatibility while achieving a 34.2% reduction in water absorption and decreased apparent porosity from 9.03% to 7.85%. Tribological evaluation demonstrated superior performance stability, with CSFCM-3 maintaining friction coefficients of 0.35–0.45 across 100–350 °C and exhibiting enhanced thermal stability through a fade ratio of 14.48% and recovery ratio of 95%. The total wear rate showed significant improvement, reducing by 26.26% to 3.433 × 10⁻⁷ cm³ (N·m)⁻¹ compared to untreated specimens. Microscopic analysis confirmed that the optimized silane modification promoted the formation of stable secondary plateaus and uniform wear patterns, contributing to enhanced tribological performance. This investigation establishes an effective approach for developing high-performance friction materials through precise control of silane treatment parameters. The findings demonstrate the potential for developing sustainable friction materials with enhanced performance characteristics, offering new pathways for eco-friendly material design that effectively utilizes agricultural waste resources. Full article

A large amount of cattle manure is generated in the US every year, creating challenges for ecosystem sustainability while offering opportunities for value-added products through biorefinery. While methods like land application, composting, and anaerobic digestion are well established for treating cattle manure, advanced biorefinery technologies are needed to enhance the utilization of cattle manure or its digestate. Based on the composition of cattle manure, this review discusses technologies for the biorefinery of cattle manure or its digestate, including the recovery of fiber and protein, and the production of platform chemicals, such as volatile fatty acids, lactic acid, medium-chain carboxylic acids, ethanol, single-cell protein, and lipids. Additionally, this review discusses process integration for simultaneously producing multiple value-added products from cattle manure using various biorefinery technologies. By offering insights into different approaches, this review aims to support the development of integrated biorefinery processes, thereby improving the economic and environmental sustainability of cattle manure management. Full article

The incorporation of monensin into cattle diets can significantly alter the physicochemical properties of excreted manure, potentially affecting waste management and treatment systems given the persistence of substantial concentrations of ionophores in the effluent. This study assessed the impact of monensin on the compositional characteristics of cattle manure and its implications for anaerobic digestion efficiency, with and without the separation of manure fractions across two hydraulic retention times (HRTs). Manure samples were collected from cattle fed with doses of monensin at 0, 1.8, 3.6, 5.4, and 7.2 mg per kg of dry matter intake. The HRTs investigated were 20 days (HRT20) and 30 days (HRT30). Increasing monensin inclusion in the diets resulted in a notable decrease in the quantities of total solids (TSs), volatile solids (VSs), and neutral detergent fiber (NDF) per animal per day, accompanied by an increase in lignin content and mass. Fraction separation during anaerobic digestion enhances the reduction of TSs, VSs, and NDF, thereby optimizing biogas and methane production potentials and elevating methane concentrations. The presence of monensin correlated with the reduced degradation of organic components during the anaerobic digestion process. To maximize the efficiency of the anaerobic digestion of manure from cattle diets supplemented with monensin, a 30-day HRT combined with fraction separation is recommended. This approach can enhance biogas yield and methane content, thereby improving the sustainability and efficacy of waste treatment processes. Full article

Agricultural production in the Azores primarily focuses on the livestock sector, notably, dairy production, where cows graze year-round in a rotational system. To maintain pasture productivity, farmers often rely on synthetic nitrogen fertilizers, which have adverse environmental impacts like ammonia emissions and nitrate leaching. Alternatively, nitrogen-fixing crops like legumes are explored as green manures to enhance soil quality and reduce dependence on chemical fertilizers. The traditional practice of using mixed forages of legumes and grasses, known as “outonos” or intercrops, has been crucial but is declining over time. These mixtures include plants such as lupins, Vicia faba, oats, and vetch, noted for their adaptability and nitrogen-fixing ability. Due to the high perishability of these crops, effective conservation strategies like ensiling are essential to preserve forage nutritional quality through controlled fermentation. This study evaluates the productivity and quality of intercrop forages in the Azores, focusing on fresh samples and silage prepared with wilting times of 0, 24, 48, and 96 h, followed by comprehensive chemical analyses. Results showed significant changes in fiber components (neutral detergent fiber, acid detergent fiber, and acid detergent lignin) with increased wilting time, leading to reduced digestibility. However, wilting improved dry matter content. Full article

We used published data consisting of 263 treatment mean observations from beef cattle and dairy steers and heifers, in which CH₄ was measured via chambers or head boxes, to evaluate relationships between enteric CH₄ production and dry matter intake (DMI) and dietary components. Daily DMI was positively related (slope = 15.371, p < 0.001) to total daily production (g/d) of CH₄ (r² = 0.821). Among chemical components, dietary neutral detergent fiber (NDF) concentration was the most highly related (r² = 0.696; slope = 0.2001; p < 0.001) to CH₄ yield (g/kg of DMI), with strong relationships also noted for dietary starch:NDF ratio (r² = 0.662; slope = −2.4587; p < 0.001), starch (r² = 0.495; slope = −0.106; p < 0.001), and the proportion of metabolizable energy relative to gross energy (r² = 0.561; slope = −23.663; p < 0.001). The slope (−0.5871) and intercept (22.2295) for the dietary ether extract vs. CH₄ yield were significant (p < 0.001), but the relationship was highly variable (r² = 0.150). For dietary crude protein concentration, the slope for CH₄ yield was not significant (−0.0344; p < 0.381) with an r² value near zero. Decreasing DMI by programming body weight gain or restricting feed intake could decrease CH₄ production in confined cattle, but these approaches might negatively affect growth performance and product quality, potentially negating positive effects on CH₄ production. Feeding higher-quality forages or using grazing management systems that decrease dietary NDF concentrations or substituting grain (starch) for forage should decrease both CH₄ yield from enteric production and manure CH₄ production via increased digestibility. Effects of feeding management and diet formulation strategies should be additive with other mitigation approaches such as feed additives, allowing the cattle industry to achieve maximal decreases in enteric CH₄ production, while concurrently maintaining optimal beef production. Full article

Guar (Cyamopsis tetragonoloba) is an annual legume tolerant to drought. Guar meal (GM) is a protein- and carbohydrate-rich co-product generated after the mechanical separation of the endosperm from the germ and hull of guar seed. GM has received considerable interest in animal feed as an alternative to soybean meal (SM). In this study, we aimed to assess the nitrogen (N) balance indicators, performance, carcass traits, and main greenhouse gas (GHG) emissions resulting from enteric fermentation (E-CH₄) and manure (M-CH₄ and N₂O). Two tests were performed: (i) a biological trial on 45 pigs (15 animals/group) and (ii) a digestibility test in metabolism cages (N = 15, 5 replicates/group). Three different diets were given to the pigs: one diet was based on 0% GM (SM diet); in the second, GM-50%, GM replaced 50% of the SM; and the third was GM-100%, in which GM fully replaced the SM. The GM and SM diets were analyzed for their proximate composition. A model based on prediction equations was used to estimate the GHGs. GM up to 10% in the diets of finishing pigs did not significantly impact growth performance or carcass traits, although a slight increase in neutral detergent fiber (NDF) was observed. GM up to 10% improved N digestibility (p < 0.0001), net protein utilization (p < 0.0001), the biological value of protein, coefficients of metabolizability, and the coefficient of the total tract’s apparent digestibility. Irrespective of its dietary proportion, GM decreased total nitrogen output (TNO, p = 0.11). A highly significant impact was noted for N₂O and E-CH₄ (for DM, p < 0.0001), as well as a significant impact for E-CH₄, expressed as g CO₂ Eq (p = 0.007), and g CO₂ Eq. LU (livestock unit, p = 0.005), also reported as ADG (p = 0.024). Manure, M-CH₄, was not significantly influenced. In conclusion, GM can replace up to 100% SM and is thus a valuable byproduct that does not alter animal performance and can positively impact N₂O and E-CH₄. Full article

Broiler deep litter is composed of cellulose, manure, feathers, and feed, and after reuse through several flocks, may be used as a fertilizer. Alternative litter materials such as plastic flooring have been researched but little is known about the characteristics of the waste produced. We analyzed the properties of broiler manure from different flooring systems and assessed its potential for biodigestion. Broiler chicks (1500 one-day-old) were reared using five flooring materials: wood shavings (WS); plastic floor (PP); 50% shavings + 50% plastic floor (PP + WS); plastic floor with antimicrobial additive (PPA); and 50% shavings + 50% plastic floor with an antimicrobial additive (PPA + WS). Waste collection was done at 14, 28, and 42 days of growth. The total residue (kg) produced was quantified on day 42. The total solids (TS), volatile solids (VS), neutral detergent fiber (NDF), acid detergent fiber (ADF), nitrogen (N), and pH were analyzed at 14, 28, and 42 days of growth, using the residue coefficient (RC) on the 42nd day. The contents of ST, SV, NDF, ADF, C, and N were determined for anaerobic digestion. The concentrations of O₂, CO₂, and CH₄ produced by anaerobic digestion were analyzed. The flooring material did not affect the volatile solids’ contents. The lowest NDF and ADF were found in plastic floor residues. The pH of the manure linearly increased over time as the birds grew. The presence of wood shavings in the manure was decisive for the production of biogas. The treatments with the plastic floor without shavings obtained the highest N content with less residue. Plastic flooring with wood shaving is not recommended as it increases waste generation. The use of plastic flooring reduced the amounts of waste generated and promote a greater yield of biogas with the anaerobic digestion of excreta. Full article

Permanent grasslands represent the main terrestrial ecosystem and serve as an important global reservoir of biodiversity, providing a wide range of benefits to humans and ecosystems. The effects of environment on permanent meadows (in our survey, they were centuries-old meadows that had not been plowed, mowed, or fertilized with manure) production have been adequately investigated in literature. However, plant species composition impact on potential feed value of first cut has still to be understood, in particular regarding different agronomic management. Our field trial was carried out in five farms, in a territory involved in the value chain of the Parmigiano Reggiano PDO (Val d’Enza, Northern Italy), over a two-year period (2017–2018). Differences in botanical composition, biomass, and Pastoral Value index (PV), which synthesizes grassland yield and nutritional parameters, were investigated in depth. The herbage dry matter (DM) yield was affected by year, farm, and their interaction factors. Its highest value across the two years was recorded in farm 5 (11.7 tons of DM ha⁻¹), which applied the highest rate of nitrogen fertilization. The botanical composition of the first cut has favored the presence of both Poaceae and ‘other species’ (each one around 40 plants per transect) compared to Fabaceae (seven plants per transect). However, higher numbers of Fabaceae plants (13 and 10) plausibly determined increases in PV in farms 3 and 5 (56.4 and 58.7, respectively). Although differences were observed among the most important nutritional parameters of grassland (crude protein, digestible and undigested neutral detergent fiber contents), suitable net energy for lactation (NE_L) values for feeding lactating cows were always recorded during the two years of survey. The present study provides a contribution of knowledge on how the botanical composition of permanent meadows may affect their potential nutritive value as fresh herbage for feeding dairy cows. Considering these results, the agronomic management should seek a level of plant biodiversity that at the same time might guarantee satisfactory yield and feed value, also in a context of climate change. Full article

The use of organic amendments is seen to be a promising method for enhancing crop productivity and soil health. Therefore, this study was performed for two consecutive years (2019 and 2020) to determine the effects of organic biochar (BC), sugar industry press mud (MUD), and poultry manure (PM) combined with inorganic amendments on the yield and nutritional quality of forage sorghum at the College of Agriculture, Bahauddin Zakariya University, Bahadur sub-campus, Layyah, Pakistan. The treatments were comprised of the following: control (no inorganic or organic amendments added); recommended dose of NPK (59:72:30 kg ha⁻¹); half dose of NPK (29.5:36:15 kg ha⁻¹); recommended dose of poultry manure (PM) at 5 t ha⁻¹; recommended dose of press mud (MUD) at 40 t ha⁻¹; recommended dose of biochar (BC) at 11 t ha⁻¹; BC + half NPK; MUD + half NPK; PM + half NPK; PM + BC + half NPK; PM + MUD + half NPK; BC + MUD + half NPK; PM + BC + MUD + half NPK. The treatments were carried out in a triplicate randomized complete block design. Results revealed that combined application of PM + BC + MUD + 1/2 NPK significantly enhanced the plant height (201 cm), number of leaves (17), stem diameter (18 mm), stem dry weight (201.7 g), leaf dry weight (30.4 g), leaf area (184.3 cm²), green forage yield (31.8 Mg ha⁻¹), and dry biomass yield (12.7 Mg ha⁻¹) compared with the control treatment. Forage quality traits, including crude protein (CP), brix percentage, acid detergent fiber (ADF), and acid detergent lignin (ADL), showed maximum value with the combined application of PM + BC + MUD + 1/2 NPK. ADF and ADL are linked with lower digestibility; therefore, it was concluded that the combined application of PM + BC + MUD + 1/2 NPK can improve the productivity, dry biomass yield, and CP of sorghum, but reduces the digestibility under semi-arid conditions, such as those in Central Pakistan. Full article

Sunn hemp (SH, Crotalaria juncea, L.) is a tropical multiple-purpose legume. The green manure SH (GMSH) crop might display protein ecology in sustaining ruminants; however, its silage features remain unclear. To efficiently prepare GMSH crop silage, additive treatments consisting of control (no additive, CON), molasses (MO), Acremonium cellulase (AC), and Lactobacillus casei TH14 strain inoculant (TH14) were implemented using a completely randomized design. Repeated measurements were done after silage (AE conditions) in a small-scale silo system for 120 days and after aerobic instability (AE + AIS conditions). Briefly, ensiling loss and aerobic stability ranged from 150 to 175 g/kg and 8.3 to 104 days, respectively. In AE conditions, the pH ranged from 4.33 to 5.74, and MO or AC was desirable (p < 0.01) for lactic acid fermentation. AC reduced the fiber contents. MO increased soluble non-protein nitrogen by decreasing insoluble nitrogen. TH14 increased the ammonia nitrogen level and in vitro methane production. In AE + AIS conditions, AC led to more air damage to the chemical compositions and reduced digestibility in vitro. The results show that an optimization of additives could effectively modify GMSH crop silage to make it a good protein roughage source; however, more studies are required for effectively feeding ruminants. Full article

This paper reviews the chemical hydrolysis processes of dairy manure fiber to make its sugar accessible to microorganisms during anaerobic digestion and identifies obstacles and opportunities. Researchers, so far, investigated acid, alkali, sulfite, and advanced oxidation processes (such as hydrogen peroxide assisted by microwave/ultrasound irradiation, conventional boiling, and wet oxidation), or their combinations. Generally, dilute acid (3–10%) is less effective than concentrated acid (12.5–75%), which decrystallizes the cellulose. Excessive alkaline may produce difficult-to-degrade oxycellulose. Therefore, multi-step acid hydrolysis (without alkaline) is preferred. Such processes yielded 84% and 80% manure-to-glucose and -xylose conversion, respectively. Acid pretreatment increases lignin concentration in the treated manure and hinders subsequent enzymatic processes but is compatible with fungal cellulolytic enzymes which favor low pH. Manure high alkalinity affects dilute acid pretreatment and lowers glucose yield. Accordingly, the ratio of manure to the chemical agent and its initial concentration, reaction temperature and duration, and manure fineness need optimization because they affect the hydrolysis rate. Optimizing these factors or combining processes should balance removing hemicellulose and/or lignin and increasing cellulose concentrations while not hindering any subsequent process. The reviewed methods are neither economical nor integratable with the on-farm anaerobic digestion. Economic analysis and energy balance should be monolithic components of the research. More research is required to assess the effects of nitrogen content on these processes, optimize it, and determine if another pretreatment is necessary. Full article

Bacillus megaterium is an ideal microecologics in the feed industry. BM1259 was already isolated from chicken manure and the whole-genome sequencing was also analyzed in our previous study. However, few studies concentrated on dietary supplementation with BM1259 in young ruminants and especially its effect on Holstein bull calves have not been reported. Hence, this experiment was conducted with the aim to evaluate the effects of BM1259 on growth performance, nutrient digestibility, rumen fermentation, and blood biochemical parameters in Holstein bull calves. Twenty-four healthy Holstein bull calves with the initial age of 90 days old and a similar body weight (115 ± 6.5 kg) were selected and randomly allocated into two groups with one Holstein bull calf in each pen (2.5 m × 2.2 m). Holstein bull calves in the control group (COG) were fed a basal total mixed ration (TMR), while experimental treatments (BMG) were fed with the TMR diet supplemented with 12 g/head/day of BM1259 powder (1 × 10¹⁰ cfu/g) separately. Results showed that (1) the average daily gain and dry matter intake of the BMG were significantly higher than those of the COG (p < 0.01), increased by 12.5% and 8.79%, respectively, during the 4–8 weeks after the addition of 12 g/head/day of BM1259; from 0 to 8 weeks, ADG (p < 0.05) and DMI (p < 0.05) in the BMG were significantly higher than those in the COG, increased by 14.9% and 6.04%, respectively. (2) At the end of the fourth week, the apparent digestibility of crude protein and neutral detergent fiber in the BMG was significantly higher than that in the COG (p < 0.05), increased by 5.97% and 6.70%, respectively; at the end of the eighth week, the apparent digestibility of crude protein and neutral detergent fiber was significantly higher than that of the COG (p < 0.01), increased by 5.88% and 10.26%, respectively. (3) At the end of the eighth week, the rumen fluid pH (p < 0.05), MCP (p < 0.05), and acetate (p < 0.05) in the BMG were significantly higher than those in the COG, increased by 9.03%, 19.68%, and 12.74%, respectively; at the end of the fourth and eighth week, NH₃-N concentration in the BMG was significantly lower than that in the COG, with a decrease of 21.81% and 16.40%, respectively. (4) At the end of the fourth (p < 0.05) and eighth week (p < 0.05), the glutamate content of the rumen fluid of the Holstein bull calves in the BMG was significantly higher than that in the COG, increased by 13.21% and 14.32%, respectively; at the end of the fourth week, the contents of glutamate in the serum (p < 0.05), urine (p < 0.05), and feces (p < 0.05) of the Holstein bull calves in the BMG were significantly lower than those in the COG, decreased by 25.76%, 33.87%, and 9.23%, respectively; at the end of the eighth week, the contents of glutamate in the serum, urine, and feces of the Holstein bull calves in the BMG were significantly lower than those in the COG (p < 0.01), decreased by 26.69%, 27.94%, and 11.11%, respectively. (5) After adding 12 g/head/day of BM1259, the urine ammonia–nitrogen content of the BMG was extremely significantly lower than that of the COG at the end of the fourth and eighth week (p < 0.01), decreased by 54.60% and 40.31%, respectively. (6) After adding 12 g/head/day of BM1259, there was no significant effect on the level of blood biochemical parameters of the Holstein bull calves. This study demonstrates that BM1259 can be applied as a potential microecologics to improve growth performance, nutrient digestibility, rumen fermentation, and nitrogen utilization in Holstein bull calves. Full article

Fodder galega (Galega orientalis Lam.) is a long-lived perennial legume grown for forage and green manure in northern and central Europe. It is distinguished by a high proportion of leaves in the aboveground mass, tall hollow stems, and rhizomes. A method to harvest galega allowing the separation of leaves, upper stem fragments, and inflorescences from the thick, woody parts of the stem is therefore justified. This would significantly expand the possibilities of using galega herbage, including as feed for monogastric animals. A modified flail chopper (MFC), a machine originally used for harvesting green forage, was used for the fractionated harvest of galega. The aim of this study was to evaluate the yield potential of various plant fractions of the first and second cut of fodder galega at three development stages and their nutritive value when harvested with an MFC. With this harvest system, 68–78% of the potential dry matter yield of galega was harvested, where leaves accounted for 73% of the dry matter on average, while the proportion of leaves in the yield of galega harvested with a mower (M) ranged from 50% to 60%. In the biomass of the first cut of galega, harvested with the MFC, protein concentration was 215 g kg⁻¹, 11.5% greater than in the biomass of the whole aboveground mass harvested with a mower. In the second cut, this difference, in favor of the MCF, was greater by 14%. The concentration of neutral detergent fiber (NDF) was 7.5% lower and acid detergent fiber 11% lower in galega forage harvested with the MFC compared to with a mower. Furthermore, galega harvested with MFC had 13% greater NDF digestibility and 6% greater in vitro true digestibility. The stubble remaining after MFC harvest accounted for 33% of the total height and 27% of the plant dry mass, and its qualitative composition indicates that it could be used in rations of livestock with low nutritional requirements or for non-forage purposes. Galega harvest with the MFC resulted in greater protein concentrations and overall improved nutritive value of the forage even at advanced development stages. Full article

Swine manure mono-digestion results in relatively low methane productivity due to the low degradation rate of its solid fraction (manure fibers), and due to the high ammonia and water content. The aqueous ammonia soaking (AAS) pretreatment of manure fibers has been proposed for overcoming these limitations. In this study, continuous anaerobic digestion (AD) of manure mixed with optimally AAS-treated manure fibers was compared to the AD of manure mixed with untreated manure fibers. Due to lab-scale pumping restrictions, the ratio of AAS-optimally treated manure fibers to manure was only 1/3 on a total solids (TS) basis. However, the biogas productivity and methane yield were improved by 17% and 38%, respectively, also confirming the predictions from a simplified 1st order hydrolysis model based on batch experiments. Furthermore, an improved reduction efficiency of major organic components was observed for the digester processing AAS-treated manure fibers compared to the non-treated one (e.g., 42% increased reduction for cellulose fraction). A preliminary techno-economic analysis of the proposed process showed that mixing raw manure with AAS manure fibers in large-scale digesters could result in a 72% increase of revenue compared to the AD of manure mixed with untreated fibers and 135% increase compared to that of solely manure. Full article