Search Results (41)

The archaeological complex of the Benzú rock shelter and cave, located in Ceuta (Spain), represents a heritage site of significant scientific and historical value that is currently at risk due to natural processes and, in particular, the activity of a nearby quarry. This site has been occupied from the Palaeolithic to the Bronze Age and consequently has been the subject of systematic research since 2002, focusing on its stratigraphic sequence, lithic technology, exploitation of marine resources, and the connection between both shores of the Strait of Gibraltar. With the aim of preserving this endangered heritage, a methodology based on advanced digital technologies such as photogrammetry, 3D laser scanning, and GNSS georeferencing has been implemented. These tools have enabled the creation of high-precision, three-dimensional models of the rock shelter and the cave, which are useful for both documentation and monitoring of their structural condition. In addition, fracture networks have been identified, revealing a high degree of geotechnical vulnerability, exacerbated by blasting activities at the nearby quarry. The project has produced a digital twin of the site in an open access format, serving not only for preventive conservation but also for its enhancement through virtual tours, augmented reality, and accessible outreach activities. This digitalization has been essential to facilitate the access to heritage, particularly in areas that are physically difficult to access. Finally, the integration of these digital resources into institutional policies for the sustainable management of heritage is proposed, highlighting the importance of interdisciplinary approaches that combine archaeology, geotechnology, and scientific communication. The experience at Benzú is presented as a replicable model for the protection, interpretation, and dissemination of heritage sites located in fragile and threatened environments. Full article

The weathering of seafloor hydrothermal sulfides is facilitated by microbial activities, yet the specific mechanisms of different sulfide types are not well understood. Previous studies have primarily been carried out under laboratory conditions, making it difficult to accurately replicate the complex in situ conditions of deep-sea hydrothermal fields. Herein, we deployed two well-characterized pyrite (Py)-dominated and chalcopyrite (Ccp)-dominated sulfide slices, which were placed 300 m from an active venting site in the Wocan-1 hydrothermal field (Carlsberg Ridge, Northwest Indian Ocean) for an 18-month in situ incubation experiment. Microscopic observations and organic matter analyses were conducted on the recovered sulfide slices to investigate the microbial weathering features of different sulfide types. Our results demonstrated that the weathering of the Py-dominated sulfide sample was primarily mediated by extracellular polymeric substances (EPSs) through indirect interactions, whereas the Ccp-dominated sulfide sample exhibited both direct microbial dissolution, resulting in the formation of distinct dissolution pits, and indirect EPS-mediated interactions. Four distinct phases of microbe–sulfide interactions were identified: approach, adsorption, stable attachment, and extensive colonization. Furthermore, the weathering products and biomineralization structures differed significantly between the two sulfide types, reflecting their different microbial colonization processes. Our study confirms that microorganisms are crucial in seafloor sulfide weathering. These findings advance our understanding of microbial-driven processes in sulfide mineral transformations and their role in marine ecosystems. Our findings are also valuable for future research on biogeochemical cycles and for developing bioremediation strategies for deep-sea mining. Full article

Many agricultural fields are no longer sustainable due to inadequate replenishment of soil nutrients through organic and inorganic inputs, particularly in smallholder farming systems. As a result, achieving potential crop yields in these systems has proven to be difficult. Field trials were conducted in two long rainy growing seasons in 2021 and 2023 to assess the effects of urea fertilizer and cattle manure as sources of nitrogen (N) on (i) maize crop yields and (ii) soil chemical properties at two sites (Kwa Sadala and Mungushi) located in Hai district, northern Tanzania. The trials employed a randomized complete block design with three replicates, including eight treatments. The treatments were: 0 fertilizer (control), 25, 50, 75 kg N ha⁻¹ (sole urea), 12.5 kg N (urea) + 12.5 kg N (cattle manure), 25 kg N (urea) + 25 kg N (cattle manure), and 50 and 75 kg N (sole cattle manure). Results show that the highest application rate of urea (75 kg N ha⁻¹) produced the highest grain yields of 4.21 and 4.09 t ha⁻¹ in the 2021 season and 4.32 and 4.04 t ha⁻¹ in the 2023 season at Kwa Sadala and Mungushi, respectively. The application of cattle manure at the highest rates increased the soil pH by 3.15 and 2.26% at Kwa Sadala and Mungushi, respectively. Similarly, soil total N, OC, available/extractable P, and exchangeable K increased by 100%, 56.3%, 52.36%, and 19.67%, respectively, at Kwa Sadala and by 16.67%, 18.13%, 20.95%, and 6.76%, respectively, at Mungushi. The use of urea alone at the higher rates or in combination with cattle manure at 50% each resulted in the highest net benefit (NB) in all sites. The findings from this study suggest that a comprehensive approach to managing soil nutrients, such as combining inorganic and organic inputs, may improve crop yields while maintaining soil health. Full article

This paper explores the application of Airborne Laser Scanning (ALS) technology in the investigation of the medieval Norman site of Castel Fenuculus, in the lower Calore Valley, Southern Italy. This research aims to assess the actual potential of the ALS dataset provided by the Italian Ministry of the Environment (MATTM) for the detection and visibility of archaeological features in a difficult environment characterised by dense vegetation and morphologically complex terrain. The study focuses on improving the detection and interpretation of archaeological features through a systematic approach that includes the acquisition of ALS point clouds, the implementation of classification algorithms, and the removal of vegetation layers to reveal the underlying terrain and ruined structures. Furthermore, the aim was to test different classification and filtering techniques to identify the best one to use in complex contexts, with the intention of providing a comprehensive and replicable methodological framework. Finally, the Digital Elevation Model (DTM), and various LiDAR-derived models (LDMs), were generated to visualise and highlight topographical features potentially related to archaeological remains. The results obtained demonstrate the significant potential of LiDAR in identifying and documenting archaeological features in densely vegetated and wooded landscapes. Full article

Background/Objectives: Poxviruses are large DNA viruses that replicate in the host cytoplasm without a nuclear phase. As vaccine vectors, they can package and express large recombinant cassettes from different positions of their genomic core region. We present a comparison between wildtype modified vaccinia Ankara (MVA) and isolate CR19, which has significantly expanded inverted terminal repeats (ITRs). With this expansion, a site in wildtype MVA, called deletion site (DS) IV, has been duplicated at both ends of the genome and now occupies an almost central position in the newly formed ITRs. Methods: We inserted various reporter genes into this site and found that the ITRs can be used for transgene expression. However, ITRs are genomic structures that can rapidly adapt to selective pressure through transient duplication and contraction. To test the potential utility of insertions into viral telomers, we inserted a factor from the cellular innate immune system that interferes with viral replication as an example of a difficult transgene. Results: A site almost in the centre of the ITRs can be used for transgene expression, and both sides are mirrored into identical copies. The example of a challenging transgene, tetherin, proved to be surprisingly efficient in selecting candidate vectors against the large background of parental viruses. Conclusions: Insertion of transgenes into ITRs automatically doubles the gene doses. The functionalisation of viruses with tetherin may accelerate the identification and generation of recombinant vectors for personalised medicine and pandemic preparedness. Full article

Building Information Modelling (BIM) integration with the Internet of Things (IoT) is progressing. The high level of BIM maturity involves using sensor data to manage processes or objects. The article presents the process of creating a telemetry connection between the BIM model and a Radio-Frequency Identification (RFID) sensor in the context of gaining access to various parts of a building. The process of creating a connection using an experimental set based on a microcontroller board for RFID reader support is described. The set was programmed using multiple programming languages and artificial intelligence. The article presents a unique process of connecting an RFID reader with BIM using a simple model that can be replicated in other contexts (e.g., gaining access to different parts of a construction site). Previous research shows that the unidirectional connection of IoT sensors with BIM models is not difficult. Instead, real-time bidirectional and stable connection (telemetry) is problematic. The authors undertook to fill this research gap using a proprietary IoT kit, programming sequence, and lightweight communication protocol. The paper makes a significant contribution to the discussion and understanding of BIM-IoT technology integration. The article also includes the limitations and possibilities for further development of such a connection. Full article

Enterovirus genomic replication initiates at a predicted RNA cloverleaf (5′CL) at the 5′ end of the RNA genome. The 5′CL contains one stem (SA) and three stem-loops (SLB, SLC, SLD). Here, we present an analysis of 5′CL conservation and divergence for 209 human health-related serotypes from the enterovirus genus, including enterovirus and rhinovirus species. Phylogenetic analysis indicates six distinct 5′CL serotypes that only partially correlate with the species definition. Additional findings include that 5′CL sequence conservation is higher between the EV species than between the RV species, the 5′CL of EVA and EVB are nearly identical, and RVC has the lowest 5′CL conservation. Regions of high conservation throughout all species include SA and the loop and nearby bases of SLB, which is consistent with known protein interactions at these sites. In addition to the known protein binding site for the Poly-C binding protein in the loop of SLB, other conserved consecutive cytosines in the stems of SLB and SLC provide additional potential interaction sites that have not yet been explored. Other sites of conservation, including the predicted bulge of SLD and other conserved stem, loop, and junction regions, are more difficult to explain and suggest additional interactions or structural requirements that are not yet fully understood. This more intricate understanding of sequence and structure conservation and variability in the 5′CL may assist in the development of broad-spectrum antivirals against a wide range of enteroviruses, while better defining the range of virus isotypes expected to be affected by a particular antiviral. Full article

Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigate tissue and cellular tropism of three EV-C genotypes, EV-C99, CVA-13, and CVA-20. Clinical isolates were obtained within the two passages of culture on Caco2 cells, and all three viruses were replicated in both the human airway and intestinal organotypic cultures. We did not observe differences in viral replication between fetal and adult tissue that could potentially explain the preferential infection of infants by EV-C genotypes. Infection of the airway and the intestinal cultures indicates that they both can serve as entry sites for non-polio EV-C. Ciliated airway cells and enterocytes are the target of infection for all three viruses, as well as enteroendocrine cells for EV-C99. Full article

Entry retaining via roof cutting is a new longwall mining method that has emerged in recent years, and is characterized by high resource utilization and environmental friendliness. Due to the complexity of this method, a field study is commonly employed for process optimization. Roof blasting is a key operation for retaining the entry, and the current practice involves dynamically adjusting blasting parameters through on-site testing and postblasting monitoring. However, the existing literature lacks detailed descriptions of blasting operations, making it difficult for field engineers to replicate the results. In this study, based on a roof cutting project for entry retaining, a preliminary design of blasting parameters is made based on theories and on-site geological conditions. The on-site test methods and equipment for roof-cutting blasting are described in detail, and the fractural patterns under different blasting parameters are analyzed. After the retreat of the working face, the state of roof caving in the goaf is analyzed based on monitoring data, and the effectiveness of top cutting is evaluated through reverse analysis, leading to dynamic adjustments of the blasting parameters. This research provides a reproducible construction method for roof-cutting operations and establishes the relationship between blasting parameters and post-mining monitoring data. It contributes to the development of fundamental theories and systematic technical systems for entry retaining via roof cutting, offering high-quality case studies for similar geological engineering projects. Full article

Adaptive reuse has progressively become a useful approach for generating new values concerning abandoned or underused buildings, sites, and areas to the extent that the topic is no longer conceived and perceived merely as a “bricks-and-mortar” issue. Instead, it has a dual nature: (i) one that is technical, linked to the difficult balance between low costs and fostering sustainable building solutions, and (ii) one that is social, which refers to social equity, well-being, and quality of life. Within this context, urban and architectural transformations are among those human activities that play a key role in shaping the territory in an increasingly invasive acceleration of urbanization processes. However, it is precisely here that adaptive reuse can counteract such alterations. This paper proposes a sustainable, economically feasible, and replicable valorisation model for the evaluation of adaptive reuse possibilities of a particular historical–architectural heritage, which is vast and widespread in Italy and an important example of Rationalist architecture. Such a model incorporates flexible and easily replicable spatial implications precisely because it is specifically related to the characteristics of an open-plan structure. In doing so, it aims to redefine the valorisation model based on a constant dialogue between the project and market analysis; the replicability of project strategies for “families” of historic buildings; and the need for the project’s ability to change and adapt according to emerging requirements. Full article

Hepatitis E virus (HEV) is the notable causative agent of acute and chronic hepatic, renal, pancreatic, neurological, and hematopoietic blood cell infections with high risk in immunocompromised patients. Hepatic failure is mostly documented among adults, pregnant women, and patients with preexisting liver disease. HEV is a positive sense RNA virus of 7.2 kb genome size with typically three open reading frames (ORFs) which play essential roles in viral replication, genome assembly, and transcription. The mutational substitution in the viral RNA genome makes more it difficult to understand the actual relationship in the host–virus association. ORFs of HEV encode different structural and non-structural proteins and one of them is the capsid protein which is coded by ORF2. The capsid protein mediates the encapsulation of the viral genome as well as being involved in virion assembly. In the current study, the ligand-based docking approach was employed to inhibit the active amino acids of the viral capsid protein. Depending upon S-score, ADMET profiling, and drug scanning, the top ten tetrapeptides were selected as potential drug candidates with no toxicity counter to HEV receptor protein. The S-score or docking score is a mathematical function which predicts the binding affinities of docked complexes. The binding affinity of the predicted drug–target complexes helps in the selectivity of the desired compound as a potential drug. The best two selected peptides (i.e., TDGH with S-score of −8.5 and EGDE with S-score of −8.0) interacted with the active site amino acids of the capsid protein (i.e., Arg399, Gln420, and Asp444). The molecular dynamics simulations of RMSD trajectories of TDGH–capsid protein and EDGE–capsid protein have revealed that both docked complexes were structurally stable. The study revealed that these tetrapeptides would serve as strong potential inhibitors and a starting point for the development of new drug molecules against the HEV capsid protein. In future, in vivo studies are needed to explore selected peptides as potential drug candidates. Full article

DNA polymerase eta (Pol η) is a Y-family polymerase and the product of the POLH gene. Autosomal recessive inheritance of POLH mutations is the cause of the xeroderma pigmentosum variant, a cancer predisposition syndrome. This review summarizes mounting evidence for expanded Pol η cellular functions in addition to DNA lesion bypass that are critical for maintaining genome stability. In vitro, Pol η displays efficient DNA synthesis through difficult-to-replicate sequences, catalyzes D-loop extensions, and utilizes RNA–DNA hybrid templates. Human Pol η is constitutively present at the replication fork. In response to replication stress, Pol η is upregulated at the transcriptional and protein levels, and post-translational modifications regulate its localization to chromatin. Numerous studies show that Pol η is required for efficient common fragile site replication and stability. Additionally, Pol η can be recruited to stalled replication forks through protein–protein interactions, suggesting a broader role in replication fork recovery. During somatic hypermutations, Pol η is recruited by mismatch repair proteins and is essential for V_H gene A:T basepair mutagenesis. Within the global context of repeat-dense genomes, the recruitment of Pol η to perform specialized functions during replication could promote genome stability by interrupting pure repeat arrays with base substitutions. Alternatively, not engaging Pol η in genome duplication is costly, as the absence of Pol η leads to incomplete replication and increased chromosomal instability. Full article

Cassava is an important starchy root crop grown globally in tropical and subtropical regions. The ability of cassava to withstand difficult growing conditions and long-term storability underground makes it a resilient crop, contributing to food and nutrient security. This study was conducted to evaluate the performance and adaptability of exotic cassava cultivars across different environments in South Africa and to recommend genotypes for cultivation. A total of 11 cassava cultivars were evaluated at six on-farm sites, using a randomized complete block design with three replications. There were highly significant (p < 0.001) variations between genotypes, environments, and their interaction for all yield and yield-related traits studied. This indicates the need to test the genotypes in multiple environments before effective selection and commercialization can be undertaken. MSAF2 and UKF4 showed the overall best performances for most of the traits, whilst UKF9 (49.5%) and P1/19 (48.5%) had the highest dry matter yield. UKF4 (102.7 t ha⁻¹) had the highest yield and greatest root yield stability across environments. MSAF2 did not perform consistently across environments because it was highly susceptible to cassava mosaic disease (CMD). MSAF2 could be used as a donor parent to generate novel clones with large numbers of marketable roots, and high fresh root yields, if the other parent can provide effective resistance to CMD. Based on genotype and environmental mean, Mabuyeni (KwaZulu-Natal), Mandlakazi (Limpopo), and Shatale (Mpumalanga) were found to be better environments for cassava cultivation and testing. This study is a pioneer in cassava research using multiple environments in South Africa. It provides baseline information on the performance of currently available cassava clones, their adaptation to multiple sites, the identification of suitable test sites, and information on current genetic resources for a future breeding program. Full article

Accurate description of forest fuels is necessary for developing appropriate fire management strategies aimed at reducing fire risk. Although field surveys provide accurate measurements of forest fuel load estimations, they are time consuming, expensive, and may fail to capture the inherent spatial heterogeneity of forest fuels. Previous efforts were carried out to solve this issue by estimating homogeneous response areas (HRAs), representing a promising alternative. However, previous methods suffer from a high degree of subjectivity and are difficult to validate. This paper presents a method, which allows eliminating subjectivity in estimating HRAs spatial distribution, using artificial intelligence machine learning techniques. The proposed method was developed in the natural protected area of “Sierra de Quila,” Jalisco, and was replicated in “Sierra de Álvarez,” San Luis Potosí and “Selva El Ocote,” Chiapas, Mexico, to prove its robustness. Input data encompassed a set of environmental variables including altitude, average annual precipitation, enhanced vegetation index, and forest canopy height. Four, three, and five HRAs with overall accuracy of 97.78%, 98.06%, and 98.92% were identified at “Sierra de Quila,” “Sierra de Álvarez,” and “Selva El Ocote,” respectively. Altitude and average annual precipitation were identified as the most explanatory variables in all locations, achieving a mean decrease in impurity values greater than 52.51% for altitude and up to 36.02% for average annual precipitation. HRAs showed statistically significant differences in all study sites according to the Kruskal–Wallis test (p-value < 0.05). Differences among groups were also significant based on the Wilcoxon–Mann–Whitney (p-value < 0.05) for all variables but EVI in “Selva El Ocote.” These results show the potential of our approach to objectively identify distinct homogeneous areas in terms of their fuel properties. This allows the adequate management of fire and forest fuels in decision-making processes. Full article

Highly active anti-retroviral therapy (HAART) is prescribed for HIV infection and, to a certain extent, limits the infection’s spread. However, it cannot completely eradicate the latent virus in remote and cellular reservoir areas, and due to the complex nature of the infection, the total eradication of HIV is difficult to achieve. Furthermore, monotherapy and multiple therapies are not of much help. Hence, there is a dire need for novel drug delivery strategies that may improve efficacy, decrease side effects, reduce dosing frequency, and improve patient adherence to therapy. Such a novel strategy could help to target the reservoir sites and eradicate HIV from different biological sanctuaries. In the current review, we have described HIV pathogenesis, the mechanism of HIV replication, and different biological reservoir sites to better understand the underlying mechanisms of HIV spread. Further, the review deliberates on the challenges faced by the current conventional drug delivery systems and introduces some novel drug delivery strategies that have been explored to overcome conventional drug delivery limitations. In addition, the review also summarizes several nanotechnology-based approaches that are being explored to resolve the challenges of HIV treatment by the virtue of delivering a variety of anti-HIV agents, either as combination therapies or by actively targeting HIV reservoir sites. Full article