Search Results (3,149)

Background: Tissue culture might be a method supplementing traditional plant propagation in various fields, like agriculture, medicine, industry, and the active conservation of plant species. For the purpose of plant restoration, it is important that the obtained progenies are identical with the mother plants to ensure the true-to-typeness of the future population. Methods: In the present study, the stability of Aldrovanda vesiculosa regenerants obtained in vitro through phenotypic and genetic analysis was estimated. Clones of aldrovanda plants were cultivated in tissue culture in the 1/10 MS liquid medium under the same conditions for over a year, with five weeks of subculturing. Results: It was observed that two clones formed plants that displayed atypical growth structures, the shoots were shorter with many lateral shoots, and they had a lower fresh weight. They also formed fewer and smaller snap-traps, which, in the case of carnivorous plants, determines the capability of catching prey. The 35 in vitro regenerated plants and 5 specimens obtained from the natural habitat were subjected to genetic analyses with two molecular markers: start codon targeted (SCoT) polymorphism and sequence-related amplified polymorphism (SRAP). Despite the visible morphological variants, the genetic stability of all the regenerants with the individuals from natural stands was confirmed. All of them were monomorphic except three bands that were obtained for reference, where individuals were amplified with SCoT28 and me12-em13 SRAP primers. Conclusions: As shown in the presented research, it might be recommended to use different methods to evaluate the stability of in vitro cultivated plants. Full article

Miao embroidery holds significant cultural, economic, and aesthetic value. However, its transmission faces numerous challenges: a high learning threshold, a lack of interest among younger generations, and low production efficiency. These factors have created obstacles to its sustainable development. In the age of artificial intelligence (AI), generative AI is expected to improve the efficiency of pattern innovation and the adaptability of the embroidery industry. Therefore, this study proposes a Miao embroidery pattern generation and application method based on Stable Diffusion and low-rank adaptation (LoRA) fine-tuning. The process includes image preprocessing, data labeling, model training, pattern generation, and embroidery production. Combining objective indicators with subjective expert review, supplemented by feedback from local artisans, we systematically evaluated five representative Miao embroidery styles, focusing on generation quality and their social and business impact. The results demonstrate that the proposed model outperforms the original diffusion model in terms of pattern quality and style consistency, with optimal results obtained under a LoRA scale of 0.8–1.2 and diffusion steps of 20–40. Generated patterns were parameterized and successfully implemented in digital embroidery. This method uses AI technology to lower the skill threshold for embroidery training. Combined with digital embroidery machines, it reduces production costs, significantly improving productivity and increasing the income of embroiderers. This promotes broader participation in embroidery practice and supports the sustainable inheritance of Miao embroidery. It also provides a replicable technical path for the intelligent generation and sustainable design of intangible cultural heritage (ICH). Full article

Background/Objectives: Involuntary migration exposes individuals to multiple losses and ruptures that profoundly affect their physical, emotional, and social well-being. This study aimed to explore the vital losses experienced by Latin American women seeking international protection, identifying key dimensions of these losses and the coping strategies they employ to support their health and well-being. Methods: The study employed a qualitative phenomenological approach, with 17 international migrant women comprising the study subjects. Data were analysed using an inductive approach and interpretative phenomenological analysis, facilitated by Atlas.ti 23.0 software. The COREQ criteria were followed. Results: The analysis revealed two central themes: the multiplicity of losses associated with migration, including loss of identity, emotional deterioration, disruption of family and community ties, economic instability, and loss of sense of belonging; and hidden gains, encompassing processes of gratitude, spiritual strength, and personal transformation. Conclusions: The findings highlight the complexity of both the losses and the hidden gains associated with the migration experience, underscoring the need for compassionate and culturally competent healthcare. This study provides relevant evidence to improve professional support strategies for refugee women from a comprehensive and humanised perspective. Full article

As environmental conservation and community development gain importance, ecotourism has emerged as a significant segment of the global tourism industry. However, the cultural factors that drive tourist behavior in this domain remain underexplored. This research examined how power distance belief (PDB), interacts with the type of tourism content shared on social media (conspicuous versus experiential) to influence travelers’ ecotourism intentions. To test our hypotheses, we conducted two experimental studies using a 2 (PDB: high vs. low) × 2 (tourism content type: conspicuous vs. experiential) between-subjects design. Participants for both experiments (N = 480) were recruited through an online survey platform. In the experiments, participants’ PDB was situationally primed, and tourism content type was manipulated using specifically created fictitious posts adapted from a real social media platform. Other key variables were measured using validated multi-item scales. Data were analyzed using analysis of variance (ANOVA) and moderated mediation analysis (PROCESS Model 15). The findings reveal that travelers with high PDB show higher ecotourism intentions when exposed to conspicuous content, whereas travelers with low PDB exhibit higher intentions when exposed to experiential content. This interactive effect is mediated by travelers’ social comparison motives. These findings offer novel insights into the motivations underlying ecotourism behavior by identifying distinct pathways through which social media can promote sustainable tourism behaviors, and provide practical guidance for eco-destination managers to design targeted marketing strategies that encourage sustainable tourism practices across different consumer segments. Full article

Traditional culture plays a vital role in shaping national identity and emotional belonging, making it imperative to explore innovative strategies for its digital preservation and engagement. This study investigates how interaction design in cultural digital games influences users’ emotional experiences and cultural understanding. Centering on the Chinese intangible cultural heritage puppet manipulation, we developed an interactive cultural game with three modes: gesture-based interaction via Leap Motion, keyboard control, and passive video viewing. A multimodal evaluation framework was employed, integrating subjective questionnaires with physiological indicators, including Functional Near-Infrared Spectroscopy (fNIRS), infrared thermography (IRT), and electrodermal activity (EDA), to assess users’ emotional responses, immersion, and perception of cultural content. Results demonstrated that gesture-based interaction, which aligns closely with the embodied cultural behavior of puppet manipulation, significantly enhanced users’ emotional engagement and cultural comprehension compared to the other two modes. Moreover, fNIRS data revealed broader activation in brain regions associated with emotion regulation and cognitive control during gesture interaction. These findings underscore the importance of culturally congruent interaction design in enhancing user experience and emotional resonance in digital cultural applications. This study provides empirical evidence supporting the integration of cultural context into interaction strategies, offering valuable insights for the development of emotionally immersive systems for intangible cultural heritage preservation. Full article

Intelligence, academic achievement and an unfavorable discrepancy between them (i.e., underachievement) have been proposed to influence students’ subjective well-being. However, previous research on these effects remains scarce and inconsistent. The present study examined the associations between said variables in a sample of 695 fifteen-year-old students in Germany, differentiating between those with and without a migration background. Our findings unexpectedly revealed that students with a migration background reported higher life satisfaction than those without a migration background. Intelligence was unrelated to life satisfaction, regardless of migration background. Academic achievement, measured by the grade point average, was positively associated with life satisfaction among students without a migration background but showed no such relationship in students with a migration background. Segmented regression analyses further indicated that an unfavorable discrepancy between IQ and grade point average, reflecting underachievement, was associated with lower life satisfaction among students with a migration background but not among those without. These findings partially challenge previous research and theoretical assumptions. We discuss the theoretical and practical implications of our findings for educational policy and emphasize the importance of targeted interventions to address underachievement in students with a migration background. Our findings suggest that poor academic performance can have a particular impact on well-being in this group. Accordingly, interventions aimed at reducing the achievement gap of those students should not only target cognitive and academic skills but also promote emotional support, cultural inclusion and social integration in the school environment. Full article

Food tourism is growing in travel experiences, with tourists increasingly drawn to culturally authentic and aesthetically engaging food. Understanding the psychological and experiential factors shaping these preferences is crucial for enhancing tourist visit intentions. Therefore, this study examines the factors such as attitudes (ATT), subjective norms (SN), perceived behavioural control (PBC), and food aesthetic experience (FAE) that influence tourists’ behavioural intentions towards food preferences (BIFP) in Goa, India. The research extends the theory of planned behaviour (TPB) by incorporating food aesthetic experiences as a mediating factor. A quantitative approach was employed, involving a structured questionnaire based on a five-point Likert scale, administered to 217 tourists visiting Goa, India, who were selected through a random sampling method. Data were analysed using Partial Least Squares–Structural Equation Modelling (PLS-SEM) through SmartPLS 4.0. Results show that attitudes, subjective norms, perceived behavioural control, and food aesthetic experience significantly influence tourists’ behavioural intentions towards food preferences. Food aesthetic experience emerged as a strong mediator, particularly between attitudes, perceived behavioural control, and tourists’ behavioural intentions towards food preferences. These findings highlight the role of sensory appeal in shaping culinary choices. The study concludes that a holistic approach, supported by cultural education and stakeholder collaboration, can enrich tourist experiences and support food tourism development. Full article

Cultural and religious norms significantly influence reproductive decisions, including prenatal testing and pregnancy termination. We conducted a cross-sectional study among Muslim women living in mixed Jewish-Arab cities compared to those in predominantly Arab cities in Israel. Data for all co-variates were obtained through participants’ self-reports by an online questionnaire of 36 items for adequate content validity between July 2022 and June 2023. In total, 1081 Israeli Muslim women aged 18–49 years were enrolled. Religious affiliation has been shown to influence individuals’ attitudes toward abortion, with members of religious communities often holding more restrictive or negative views on the subject. Muslim women residing in mixed Jewish-Arab cities demonstrate significantly higher uptake of both non-invasive and invasive prenatal testing, as well as pregnancy terminations following abnormal diagnoses, compared to those in predominantly Arab cities (p < 0.001), which indicates that proximity to Jewish communities, greater availability of health services, and exposure to more permissive social norms in mixed cities may reduce religious and cultural barriers, thereby facilitating more informed reproductive decisions. Older women (≥35) in mixed cities had a higher tendency to birth a child with abnormalities in the past than Arab cities (80.9% vs. 70.0%, respectively), However, women who lived in mixed cities underwent more non-invasive (87.2% vs. 64.8%, respectively), invasive prenatal tests (85.1% vs. 69.7%, respectively), and pregnancy termination (88.3% vs. 64.1%, respectively) than those in Arab cities, p < 0.001. Our findings indicate the importance of gaining a deeper understanding of the relations between religious convictions, cultural standards, and medical practices that should contribute to reducing the prevalence of genetic disorders with their associated adverse impact on families, communities, and healthcare systems. Full article

Suicide is a leading cause of death among construction workers, particularly younger and lower-skilled employees. Barriers such as stigma, low mental health literacy, and traditional masculine norms hinder help-seeking in this male-dominated sector. Few mental health interventions are tailored to this context. This study developed a co-designed, theory-informed training to improve mental health literacy, reduce stigma, and increase help-seeking among construction workers in Ireland. Using the Medical Research Council’s framework, the training was developed with the Theory of Planned Behavior (TPB), Behavior Change Techniques, and extensive stakeholder co-design. Two systematic reviews, a broad literature review, and focus groups with industry managers informed the content and structure. The training will be pilot-tested using validated measures: the Literacy of Suicide Scale (LOSS), the Stigma of Suicide Scale (SOSS), and the General Help-Seeking Questionnaire (GHSQ), the results of which will be the subject of a separate study. CAIRDE is a promising, evidence-based training that addresses key mental health barriers in Irish construction. Embedding the TPB within a co-design methodology has resulted in the development of a training program that is underpinned by theoretical fidelity and cultural relevance and provides a framework for other male-dominated industries to draw upon. Future work should address remaining challenges related to stigma and help-seeking, and explore broader implementation through integration into mandatory safety training. Full article

Objectives: Animal studies have demonstrated that mesenchymal stem cell-conditioned medium (MSC-CM) possesses various therapeutic effects, including anti-inflammatory and anti-fibrotic properties. This study investigated the efficacy and safety of administering MSC-CM as a treatment for patients with generalized fatigue. Methods: The MSC-CM used in this study was derived from human adipose tissue and umbilical cord-derived mesenchymal stem cells cultured in a medium free of animal-derived components to avoid the risk of infection. This MSC-CM has recently been shown to possess anti-inflammatory effects and has been reported to be safe for human administration. With the expectation of alleviating fatigue symptoms through its anti-inflammatory effects, it was administered to patients intravenously and by inhalation. Safety and changes in subjective symptoms were evaluated, and blood biomarkers related to inflammation and oxidative stress were measured. Results: In this trial involving 19 patients experiencing fatigue, no serious side effects were observed following MSC-CM administration. Nearly half of the patients reported symptom improvement after a single dose, and some exhibited signs of reduced inflammation. Conclusions: This report presents the first investigation of systemic MSC-CM treatment for generalized fatigue, paving the way for more targeted studies on dosage and treatment frequency. These findings offer new hope and possibilities for treating fatigue, providing valuable insights into the clinical application of MSC-CM. Full article

The Terracotta Army, an integral part of China’s cultural heritage, has suffered physical erosion like cracks and notches over time. Manual inspection methods are inefficient and subjective. This study proposes an automated defect detection system based on computer vision to enhance the efficiency and precision of detecting these defects. The system includes the following core modules: (1) high-resolution image acquisition, which ensures comprehensive and detailed data capture; (2) sophisticated image illumination processing, which compensates for varying lighting conditions and improves image quality; (3) advanced image data augmentation techniques, which enrich the dataset and improve the generalization ability of the detection model; and (4) accurate defect detection, which leverages state-of-the-art algorithms. In the experimental phase, the efficacy of the proposed approach was evaluated. Illumination-enhanced low-light images were used for data augmentation, and the generated images showed high similarity to the original images, as measured by PSNR and SSIM. The YOLOv10 algorithm was employed for defect detection and achieved average detection rates of 91.71% for cracks and 93.04% for abrasions. This research provides a scientific and efficient solution for cultural relic protection and offers a valuable reference for future research in heritage conservation. Full article

In this study, the effect of growth regulators on shoot proliferation and rooting were evaluated to develop an efficient micropropagation protocol for the Cannabis sativa L. cultivars ‘Cherry Soda’ and ‘Purple’. Apical meristems were isolated from actively growing shoots of stock plants and transferred to Driver and Kuniyuki Walnut (DKW) culture medium containing either 0.0, 0.5, 1.0, 2.0, or 5.0 μM meta-Topolin to study their shoot proliferation response. Resulting shoot cultures were transferred to medium containing varying levels of Indole Acetic Acid (IAA), Indole Butyric Acid (IBA), or Naphthalene Acetic Acid (NAA), solely or in combination, and were subjected to a 10-day dark incubation followed by a 16 h/8 h light/dark period to identify the best treatment for root production. Among the different shoot proliferation treatments studied, the maximum number of shoots was produced on the control medium that was devoid of any meta-Topolin. Cultures grown on medium containing 5.0 μM meta-Topolin exhibited hyperhydricity, where shoots appeared translucent and pale green in color; were characterized by water-soaked lesions; and leaves appeared curled and brittle in contrast to healthy looking cultures. Among the various rooting treatments studied, shoots grown in the dark for 10 days exhibited the highest frequency of rooting on medium containing 4.0 μM NAA or 6.0 μM IBA + 1.0 μM NAA. Full developed plants with a robust shoot and root system were transferred to soil, acclimatized under conditions for high humidity, and then transferred to ambient conditions in 4 weeks. The micropropagation protocol developed here allows for rapid multiplication of disease-free plants in C. sativa cultivars. Full article

Background/Objectives: Traditional models like ordinary least squares (OLS) struggle to capture non-linear relationships in children’s subjective well-being (SWB), which is associated with physical activity. This study evaluated machine learning (ML) for predicting SWB, focusing on sports participation, and explored theoretical prediction limits using a global dataset. It addresses a gap in understanding complex patterns across diverse cultural contexts. Methods: We analyzed 128,184 records from the ISCWeB survey (ages 6–14, 35 countries), with self-reported data on sports frequency, emotional states, and family support. To ensure cross-country generalizability, we used GroupKFold CV (grouped by country) and leave-one-country-out (LOCO) validation, yielding mean R² = 0.45 ± 0.05, confirming robustness beyond cultural patterns, SHAP for interpretability, and bootstrapping for error estimation. No pre-registration was required for this secondary analysis. Results: XGBoost and LightGBM outperformed OLS, achieving R² up to 0.504 in restricted datasets (sensitivity excluding affective leakage: R² = 0.35), with sports-related variables (e.g., exercise frequency) associated positively with SWB predictions (SHAP values: +0.15–0.25; incremental ΔR² = 0.06 over demographics/family/school base). Using test–retest reliability from literature (r = 0.74), the estimated irreducible RMSE reached 0.941; XGBoost achieved RMSE = 1.323, approaching the predictability bound with 68.1% of explainable variance captured (after noise adjustment). Partial dependence plots showed linear associations with exercise without satiation and slight age decline. Conclusions: ML improves SWB prediction in children, highlighting associations with sports participation, and approaches predictable variance bounds. These findings suggest potential for data-driven tools to identify patterns, such as through physical literacy pathways, informing physical activity interventions. However, longitudinal studies are needed to explore causality and address cultural biases in self-reports. Full article

The centre of origin of Bambara groundnut (BGN; Vigna subterranea L.) is Western Sub-Saharan Africa. Due to its high nutritional value and tolerance to biotic and abiotic stresses, this neglected and underutilised species has recently gained significant attention. However, BGN production faces several challenges, including a lack of quality varieties. This study describes a selected core collection based on phenotypic traits, investigates relevant selection criteria and identifies a set of landraces according to participants’ preferences. A core collection of landraces was generated, described, and then subjected to participatory varietal selection. Through individual semi-structured interviews, key selection criteria were identified. Focus group discussions were organised to explore group criteria and to support and validate information from personal interviews. The varietal selection involved choosing three landraces per participant. The results highlighted that seed colour, seed size, cultural value, market value, seed taste, storage, and seed cooking duration were the main selection criteria; however, specific trait preferences varied by gender and participants. Two of 14 selected BGN landraces were considered by the panel of evaluators to be most suitable for recommendation to growers and breeders. They can be disseminated in BGN production regions and used for plant breeding. Full article

Background: The aim of this study was to explore the intention of Jordanian patients diagnosed with hyperlipidemia to engage in physical activity. This objective was achieved via an in-depth analysis of how patient attitudes, subjective norms, and perceived behavioral control can influence patient intentions to exercise. Additionally, this research examined how sociodemographic factors and perceived barriers can impact patient participation in physical activity. Methodology: This study employed a cross-sectional approach on a convenience sample of Jordanian patients diagnosed with hyperlipidemia. To gain the required data, a 15-item questionnaire (derived from the Theory of Planned Behavior) was presented to the participants in the form of an online survey (via several platforms, including WhatsApp, Facebook, and email). Results: The results indicate that perceived behavioral control had a significant correlation with the participants’ intentions to participate in physical activity. Additionally, the findings revealed that there were no significant correlations between demographic features (age, marital status, level of education, and monthly income) and intention to engage in physical activity. However, the results ascertained the existence of several facilitators to exercise (such as financial resource availability, self-interest, beneficial weather conditions, and supportive friends or exercise partners). The most commonly reported barriers to physical activity included time constraints, work commitments, and limitations imposed by existing health conditions. Conclusions: These findings provide valuable insights that can be employed to develop physical activity programs that address the cultural needs of Jordanian patients diagnosed with hyperlipidemia and enhance their levels of physical activity. Full article