Search Results (10,637)

The spread of Bursaphelenchus xylophilus has caused a critical shortage of traditional Poria cocos cultivation materials, making bag-based substrates an urgent alternative. Yet, how substrate stoichiometry shapes nutritional quality remains unclear. Using non-targeted metabolomics combined with redundancy analysis (RDA) and weighted gene co-expression network analysis (WGCNA), we profiled P. cocos cultivated on four substrates: healthy pine logs, pine wilt wood bags, oak bags, and pine needle/branch bags. Bag-cultivated P. cocos showed significantly elevated total amino acids, poria cocos acid, and total triterpenoids, with pine wilt wood bags (P1) performing best overall. Nitrogen, phosphorus, and the N/P ratio independently drove metabolomic variation (pairwise overlap < 5%). Nitrogen-line hub metabolites were negatively correlated with amino acid content, suggesting that suppressed lipid metabolism may free carbon skeletons for the accumulation of nitrogenous nutrients. Phosphorus-line hub metabolites were positively associated with polysaccharide indices, whereas the N/P ratio in line lipid amides showed strong negative correlations with polysaccharides under phosphorus limitation. These correlational patterns are consistent with a stoichiometric resource allocation model, although direct validation through controlled-element experiments is required. These findings provide quantitative guidance for optimizing bag-substrate formulations in P. cocos cultivation. Full article

Elevated potentially toxic element (PTE) contents in soils can pose important environmental concerns, particularly in regions where natural metal enrichment associated with ultramafic and mineralized geological settings overlaps with potential anthropogenic inputs. This study evaluates the distribution, enrichment, and probable controls of As, Ni, Co, Cu, Cr, Pb, and Zn in 35 geo-referenced soil samples from the Ortaklar region, Türkiye, with the aim of providing a geochemical basis for environmental monitoring and sustainable land management. PTE contents were determined by ICP-MS (inductively coupled plasma mass spectrometry), and their degree of enrichment was evaluated using the geo-accumulation index (I_geo), contamination factor (CF), and pollution load index (PLI), supported by correlation analysis and multivariate statistical approaches. Mean contents decreased in the order Ni (1972 mg kg⁻¹) > Cr (924 mg kg⁻¹) > Cu (290 mg kg⁻¹) > Zn (188 mg kg⁻¹) > Co (122 mg kg⁻¹) > As (15.9 mg kg⁻¹) > Pb (6.8 mg kg⁻¹). Ni exhibited the highest degree of enrichment and was the dominant contributor to the overall PTE load, while Cr, Cu, and Co also showed substantial enrichment. Mean I_geo values indicated moderate to heavy enrichment for Ni (2.65) and As (2.36), and moderate enrichment for Cr (1.45) and Co (1.02). The mean PLI value of 6.99 indicates a substantial cumulative enrichment of PTEs relative to the adopted reference values. Strong associations among Ni, Cr, and Co are consistent with a dominant geogenic contribution from ultramafic/serpentinitic parent materials and their weathering products, whereas the distributions of As, Cu, and Zn suggest more complex controls involving lithology, mineralization, and potentially localized agricultural or other anthropogenic inputs. However, these statistical relationships indicate probable controls rather than definitively establishing elemental sources. The elevated total PTE contents and contamination indices identify the Ortaklar soils as requiring continued geochemical monitoring; nevertheless, they do not constitute direct evidence of human-health risk because metal speciation, mobility, bioavailability, and exposure pathways were not investigated. The findings provide a regional geochemical framework for distinguishing natural PTE enrichment from anthropogenic influences and for guiding future mineralogical, bioavailability, and spatial investigations in ultramafic and mineralized soil environments. Full article

When large liquefied natural gas (LNG) storage tanks are constructed on deep non-rock sites, soil–foundation–structure interaction (SFSI) alters the dynamic characteristics of the system and affects the actual control effectiveness of the isolation layer. However, the current understanding of the coupling mechanism between the two remains insufficient. This paper takes a 220,000 m³ full-containment LNG storage tank as the study object and establishes a three-dimensional finite element model of the tank-pile group-site system in ABAQUS. Through comparative analyses of three model configurations, namely a rigid foundation model, a non-isolated model considering SFSI, and a lead-rubber bearing (LRB) isolated model considering SFSI, the SFSI effects and LRB isolation effectiveness are systematically separated. For the SFSI effects, the deep site attenuates medium- and high-frequency content while amplifying the response around approximately 1.6 Hz through site–foundation flexibility, transforming the heightwise acceleration amplification profile from an approximately linear pattern to a curvilinear one that bulges at mid-height, with peak pile-cap accelerations increasing by 25.1–76.9% relative to the rigid-base values. For the LRB isolation performance, the introduction of LRBs shifts the dominant system frequency below 1.0 Hz and reduces the maximum tank-wall acceleration amplification factor from 2.64 to 0.81. The resulting attenuation of superstructural inertial forces leads to reductions of 49.6–82.0% in pile-head shear and 57.4–78.0% in near-head bending moment, while the outer-to-inner pile-head moment ratio decreases from 2.94 to 1.13, indicating substantially improved pile-group force uniformity. Nevertheless, the beneficial effect of isolation diminishes with depth, and internal forces at abrupt soil-stiffness interfaces remain governed by kinematic interaction that the isolation layer cannot mitigate. The findings of this study can provide references for the seismic isolation design and pile foundation seismic optimization of super-large LNG storage tanks on deep overburden sites. Full article

The increasing use of electric vehicles has led to a growing demand for rare-earth elements, particularly neodymium (Nd), which is a critical component of Nd–Fe–B permanent magnets used in traction motors. End-of-life electric vehicle motors are therefore considered promising secondary resources for Nd recovery. In this study, a pyrometallurgical process using non-ferrous smelting slag as a flux was proposed for recovering Nd from Nd–Fe–B magnets contained in waste electric vehicle motors. Steel and magnet fractions obtained from a dismantled motor were melted at approximately 1600 °C under an air atmosphere, and Fe₂O₃ was added as an oxidizing agent to promote the selective oxidation of Nd. The oxidized Nd was subsequently partitioned into the slag phase as Nd₂O₃ through metal–slag separation. The effects of the slag flux addition ratio, Fe₂O₃ content, slag flux type, and crucible material on Nd recovery behavior were systematically investigated. Increasing the fayalite-based slag flux addition enhanced Nd transfer into the slag phase, and the highest Nd recovery of approximately 80% was obtained at a slag flux addition ratio of 30 wt%. The addition of 2 wt% Fe₂O₃ was found to be suitable for promoting stable Nd oxidation and efficient slag–metal separation. The non-ferrous smelting slag exhibited chemical and phase characteristics comparable to those of synthetic fayalite slag flux, resulting in similar Nd recovery performance. In addition, the crucible material significantly affected the process stability and Nd recovery behavior. Although a carbon crucible showed relatively high Nd recovery, severe slag foaming and crucible erosion occurred during melting. In contrast, alumina and MgO crucibles provided stable process conditions with comparable Nd recovery behavior, whereas zirconia crucibles caused relatively higher Nd loss. These results demonstrate that non-ferrous smelting slag can be effectively used as a flux for the pyrometallurgical recovery of Nd from end-of-life electric vehicle motors, offering a potential route for sustainable rare-earth recycling. Full article

Photodynamic therapy (PDT) exploits photoactivated materials that generate reactive oxygen species (ROS) to induce selective cancer cell death. Nitrogen-doped carbon dots (N-CDs) have emerged as promising photosensitizers owing to their favorable optical properties, while hybridization with titanium dioxide (TiO₂) may further enhance photoinduced ROS generation through improved charge separation. Here, we synthesized a series of N-CD/TiO₂ hybrid composites with varying TiO₂ content using a hydrothermal approach and systematically investigated the relationship between their physicochemical characteristics and biological activity. The hybrid materials were characterized by Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, dynamic light scattering, and UV–visible spectroscopy. Among the formulations investigated, the composite containing 90% N-CDs and 10% TiO₂ (N-CDs10T) exhibited the smallest hydrodynamic diameter, a relatively narrow particle size distribution, favorable optical properties, and the strongest irradiation-dependent biological responses. Biological activity was evaluated in A549 lung adenocarcinoma and Kelly neuroblastoma cells. Under dark conditions, all formulations displayed relatively low intrinsic cytotoxicity. Following irradiation with 365 nm UVA light, however, N-CDs10T induced a marked increase in intracellular ROS production, activation of antioxidant response element (ARE)-dependent signaling, disruption of cell-cycle progression, apoptosis-associated cell death, and inhibition of cell proliferation and migration. Kelly cells exhibited greater sensitivity than A549 cells, with IC₅₀ values decreasing from 0.98 mg/mL under dark conditions to 0.52 mg/mL following irradiation. Collectively, these findings demonstrate that N-CD/TiO₂ hybrid composites function as photoresponsive materials that enhance ROS-mediated cytotoxicity upon light activation. Beyond demonstrating phototoxicity, this study systematically links hybrid composition with oxidative stress signaling and multiple cellular responses, providing a comprehensive biological evaluation of N-CD/TiO₂ hybrid materials. While additional studies are required to identify the predominant ROS, evaluate selectivity in non-malignant cells, and optimize activation at clinically relevant wavelengths, the present work establishes a proof of concept for the development of N-CD/TiO₂ hybrid composites for photodynamic applications. Full article

Betaine aldehyde dehydrogenase (BADH) catalyzes the final step in glycine betaine biosynthesis, but the evolutionary divergence and differential salt responsiveness of BADH homeologs in bread wheat remain unclear. We identified six TaBADH genes and analyzed their phylogenetic relationships, conserved motifs, gene structures, promoter cis-acting elements and synteny. RNA-seq and qRT-PCR were used to compare expression in salt-tolerant Jimai 60 and salt-sensitive Chinese Spring under 200 mM NaCl, and BADH activity, glycine betaine, H₂O₂ and malondialdehyde (MDA) were measured during treatment. The genes separated into chromosome group 2 and group 6 clades with distinct structural and transcriptional patterns. TaBADH-2B encoded a shorter protein and lacked several conserved motifs. Group 6 genes showed stronger salt-responsive expression in Jimai 60, with TaBADH-6D displaying the strongest and most sustained induction. Jimai 60 also showed higher BADH activity and glycine betaine accumulation and lower H₂O₂ and MDA contents at later time points. Expression of TaBADH-6D improved E. coli growth under 200 mM NaCl. These findings identify homeolog-specific divergence within the BADH wheat family and support TaBADH-6D as a candidate for plant-level functional validation. Full article

Industrial heritage tourism depends on more than the preservation of visible structures; it also requires visitors to perceive historical and cultural meaning. This study examines public perceptions of eight representative industrial heritage tourism sites in Wuhan. Drawing on Scene Theory, we developed a three-dimensional framework encompassing physical, social, and cultural scenes. We collected user-generated content (UGC) from Dianping.com and evaluated 12 perception elements using semantic network analysis and a modified Importance–Performance Analysis based on topic salience and satisfaction. Public perceptions were dominated by visual aesthetics and social-media engagement. Ecological atmosphere received favourable evaluations despite its lower salience, whereas homogeneous commercial offerings weakened the visitor experience. Era memory and industrial heritage combined relatively high salience with low satisfaction, revealing a gap between visible physical remains and the communication of industrial history and collective memory. A hierarchical analysis further showed that only 8.40% of culture-related reviews contained deep industrial narratives. We therefore propose strategies that improve spatial interpretation, embed site-specific industrial identity in commercial and social experiences, and connect heritage objects with historical processes and workers’ memories. These findings provide an evidence-based framework for strengthening cultural interpretation in the revitalization of Wuhan’s industrial heritage. Full article

The methods for determining total organic carbon (TOC) in carbonate rocks are mainly the conventional TOC method and the montmorillonite thickening method. However, both methods suffer from organic matter loss during the measurement process and inaccuracy in determining low TOC concentrations (TOC ≤ 0.5%). In this study, carbonate rock samples from the Ordovician of the Ordos Basin and synthetic standard samples were investigated using the conventional TOC method, the montmorillonite thickening method, and a newly developed elemental analysis method based on sodium polyacrylate thickening after acid digestion. The results show that gypsum-facies carbonate rocks contain organic acid salts, whereas shoal-facies and argillaceous carbonate rocks do not develop organic acid salts. For synthetic samples spiked with small-molecule organic acid salts, the relative error of the conventional TOC method exceeds 98%; for large-molecule organic acid salts, the relative error is relatively small, below 16%. The TOC measurement error of the sodium polyacrylate thickening method is lower than that of the montmorillonite thickening method. Compared with the conventional and montmorillonite methods, when carbonate rock TOC ≤ 0.5%, the sodium polyacrylate thickening method yields TOC errors mostly ≤10%, with better stability and lower error rates. The new TOC method for carbonate rocks exhibits higher accuracy and stability in determining organic carbon content, and can more objectively reflect the total organic carbon (TOC) of samples. Full article

Pinoresinol–lariciresinol reductase (PLR) catalyzes a key reductive step in plant lignan biosynthesis. Although flax (Linum usitatissimum L.) seeds are rich in lignans, the PLR gene family and its relationship with lignan accumulation during seed development remain insufficiently characterized. Here, 18 LuPLR genes were identified from the telomere-to-telomere genome assembly of the flax cultivar ‘Gaosi’ using BLASTP and HMMER searches. Their phylogenetic relationships, gene structures, conserved motifs, chromosomal distribution, duplication and syntenic relationships, promoter cis-acting elements, predicted microRNA targets, and expression profiles were analyzed. Seed lignan content at 5, 10, 20, 30, and 40 days after flowering was quantified by high-performance liquid chromatography, and candidate genes were screened using quantitative real-time PCR and Pearson correlation analysis. The LuPLR genes were unevenly distributed across eight chromosomes and exhibited substantial structural and regulatory diversity. Seed lignan content varied dynamically and reached its highest level at 40 days after flowering. LuPLR10, LuPLR11, and LuPLR16 showed positive correlations with lignan content. Among them, LuPLR10 was prioritized because its developmental expression pattern most closely paralleled lignan accumulation. Subcellular localization analysis indicated that the LuPLR10 protein was predominantly associated with chloroplasts. These findings provide a genomic framework for the flax PLR family and identify LuPLR10 as a priority candidate for further functional investigation. Full article

Organic matter enrichment in marine–continental transitional shales is influenced by volcanism, continental weathering, and paleoceanographic evolution, but their interactions during the Late Paleozoic Ice Age (LPIA) remain unclear. Mineralogical, elemental, and organic geochemical data from the Upper Carboniferous Yanghugou Formation, western Ordos Basin, were used to reconstruct volcanic input, weathering intensity, productivity, redox conditions, and organic carbon accumulation. High Hg/TOC, Zr/Al₂O₃, and Zr/Cr ratios indicate strong volcanic input during early deposition, followed by an upward decline. Chemical Index of Alteration values of 76.5–91.5 increase upward, indicating intensified chemical weathering under warmer and more humid conditions. Increasing C_org/P, Mo_EF, and U_EF values record a shift from oxic–suboxic to predominantly anoxic bottom waters. The weak relationship between productivity proxies and TOC suggests that volcanic fertilization was not the main control on organic matter enrichment. Instead, negative δ¹³C_org values, high Al₂O₃ contents, organic matter–clay associations, and reducing conditions indicate that enhanced terrestrial organic matter input and improved preservation jointly promoted organic carbon accumulation. Volcanism likely intensified climatic warming and weathering, whereas subsequent organic carbon burial contributed to atmospheric CO₂ drawdown and climatic feedbacks during the LPIA. Full article

To evaluate the influence of fiber content, temperature, and loading rate on the dynamic compression performance of ultra-high performance concrete (UHPC), orthogonal Split Hopkinson Pressure Bar (SHPB) tests were performed on 120 MPa UHPC specimens. Range analysis of the test data reveals that the steel fiber content exerts the largest range on dynamic compressive strength, with loading rate ranking second and temperature having the least effect. A three-dimensional mesoscopic finite element model that accounts for temperature-dependent behavior was developed using a modified Karagozian & Case (K&C) constitutive model together with high-temperature bond–slip degradation curves. The simulated peak stresses are generally higher than the experimental values, with a Root Mean Square Error of 9.02 MPa, a Normalized Root Mean Square Error of 4.65%, and a maximum discrepancy of 10.07%, while the major experimental failure characteristics are reasonably reproduced. Additional numerical simulations indicate that the influence of steel-fiber content becomes increasingly temperature-dependent. Within the experimentally investigated range up to 300 °C, higher fiber content generally improves dynamic response and specimen integrity. At 600~800 °C, the numerical extrapolations suggest that the reinforcing efficiency of steel fibers may be substantially reduced under the assumed temperature-dependent degradation conditions. These high-temperature trends require further experimental validation. Full article

Carboxylesterases (CXEs) participate in diverse plant metabolic processes, including isoflavone biosynthesis. However, the soybean GmCXE subfamily remains poorly characterized, especially in relation to root isoflavone accumulation and the response to Fusarium oxysporum. Here, fifty-six putative GmCXE genes were identified in the soybean genome and classified into three major phylogenetic clades. Analyses of gene structure, conserved motifs, protein domains, and promoter cis-elements revealed conserved features as well as potential functional divergence among subfamily members. Collinearity and duplication analyses indicated that segmental duplication was the main driver of GmCXE subfamily expansion. Tissue-specific expression profiling and RT-qPCR validation selected five root-expressed genes as candidates associated with isoflavone accumulation. SNP variation analysis and allelic group analysis of 209 soybean accessions further prioritized GmCXE54 as a candidate gene for root isoflavone accumulation. Allelic groups defined by a putative promoter SNP, Chr.20-rs39215413, showed significant differences in root daidzein and total isoflavone contents, with accessions carrying the C allele exhibiting higher levels of both traits than those carrying the T allele. Functional analysis in soybean hairy roots showed that GmCXE54 overexpression increased daidzein and total isoflavone accumulation. At 3 h after F. oxysporum inoculation, GmCXE2, GmCXE39, and GmCXE54 were induced, with GmCXE54 showing the strongest response in the resistant accession ZD27. These findings clarify GmCXE subfamily evolution and identify GmCXE54 as a candidate gene associated with root isoflavone accumulation and early F. oxysporum response, offering new perspectives for improving soybean isoflavone-related traits and investigating root response mechanisms. Full article

Pervious concrete must balance mechanical capacity with interconnected voids required for drainage. A three-factor, three-level Box–Behnken design examined fly ash (FA; 10–20%), metakaolin (MK; 5–15%), and nano-silica (NS; 0.5–1.5%) at a fixed total binder content of 380 kg/m³. Compressive strength, water-accessible open porosity, and the apparent permeability coefficient ranged from 16.80 to 28.20 MPa, 14.07 to 24.50%, and 2.20 to 7.29 mm/s, respectively. Quadratic models for compressive strength and the apparent permeability coefficient were statistically adequate (R² = 0.9907 and 0.9861); the porosity model showed significant lack of fit. The porosity model was retained only for local trend interpretation and excluded from optimization. In a researcher-defined design scenario that maximized strength while targeting an apparent permeability coefficient of 3.00 mm/s, desirability analysis selected a model-predicted compromise solution containing 14.038% FA, 12.556% MK, and 1.483% NS, with a predicted compressive strength and an apparent permeability coefficient of 28.233 MPa and 3.000 mm/s. The NS factor was close to the upper boundary, and the solution was not experimentally validated. Selected single-field SEM observations illustrated local differences among M5, M7, and M12, while selected-area EDS sum spectra provided only local elemental composition information. The results indicate potential for future pavement evaluation after independent validation, multi-field microstructural analysis, durability testing, and economic assessment. Full article

This research proposes an approach based on a circular economy principle for the integral valorization of Sargassum from the Mexican Caribbean. The biomass was characterized through proximal and elemental analyses, and then an oxidative pretreatment was carried out to enhance a sequential processing scheme to extract sodium alginate and use the solid waste as a substrate for biogas production via anaerobic digestion. The oxidative pretreatment successfully reduces the recalcitrant content and the concentration of heavy metals. The sodium alginate extracted from treated biomass achieves a yield higher than 20%; the characterization of the polymer via nuclear magnetic resonance showed that the mannuronic-to-guluronic ratio was between 0.34 and 0.62, indicating the potential for its use for environmental and biomedical applications. The highest yield in methane production was 328 mL CH₄/g of volatile solids, with a purity of 90%, and was achieved using the waste from alginate extraction with an inoculum-to-substrate ratio of 1:1. The experimental data presented an excellent fit to a Gompertz model (R² > 0.99). The proposed valorization pathway improves the sustainability of Sargassum management, prioritizing the recovery of high-value compounds before energy production. This circular approach provides a framework for converting environmental challenges into opportunities in the Caribbean. Full article

Existing studies on cultural landscape genes in traditional villages have largely focused on the identification of objective elements, while paying insufficient attention to the associations between tourists’ subjective perceptions, Place identity, and Satisfaction. Taking the five nationally recognized traditional villages in Wuxi, China, as case studies, this study constructs an association model linking cultural landscape gene perception, Place identity, and Satisfaction. The model was tested using partial least squares structural equation modeling based on 392 tourist questionnaires. The results indicate that Perceived Environmental Character, Perceived Settlement Morphology, Perceived Architectural Characteristics, and Perceived Cultural Attributes are all significantly and positively associated with both Place identity and Satisfaction. Place identity is also significantly and positively associated with Satisfaction. In terms of the standardized path coefficients, Perceived Settlement Morphology shows a relatively strong association with Place identity (β = 0.285), while Perceived Architectural Characteristics shows a relatively strong association with Satisfaction (β = 0.257). The model explains 41.2% of the variance in Place identity and 54.1% of the variance in Satisfaction. Both the direct paths between the four dimensions of cultural landscape gene perception and Satisfaction and the indirect paths through Place identity are statistically significant, indicating that Place identity plays a partial mediating role. This study extends Cultural Landscape Gene Theory from the identification of objective landscape elements to the levels of tourist perception and tourism evaluation, and reveals that Place identity may constitute a psychological pathway linking cultural landscape perception with Satisfaction. In practice, the perceptibility of village spatial patterns, architectural appearance, environmental characteristics, and cultural content should be comprehensively maintained to enhance tourists’ Place identity and overall experience evaluations. Full article