Search Results (4,037)

Background: Minimally invasive aesthetic procedures using atmospheric plasma devices are increasingly applied to improve skin laxity and age-related loss of firmness. These systems generate a localized plasma arc at the tissue surface, enabling controlled and spatially confined tissue interaction; however, quantitative histological data on the extent of plasma-induced tissue effects remain limited. Materials and Methods: This ex vivo study evaluated freshly collected porcine kidney, liver, and skeletal muscle tissues (n = 3 per tissue type). Tissue sublimation defects were produced using the Plasma IQ device under conditions representative of standard clinical use, applying two predefined settings (“LOW” and “HIGH”). Immediately after treatment, specimens were fixed in 10% neutral buffered formalin and processed into formalin-fixed paraffin-embedded (FFPE) blocks. Sections were stained with hematoxylin and eosin (H&E), and the diameter and depth of the sublimation zones were measured by light microscopy. Results: Plasma IQ exposure consistently produced well-demarcated superficial sublimation defects in all tissues. The HIGH setting increased the diameter of the sublimation zones compared with the LOW setting across all tissue types, whereas the depth differences were smaller and tissue-dependent. Lesions exhibited a characteristic flattened, cone-shaped morphology, with diameter exceeding depth. No histologically detectable collateral damage was observed beyond the immediate sublimation zone. Conclusions: Atmospheric plasma treatment induces controlled and spatially confined tissue sublimation with clearly defined histological boundaries and limited penetration depth. These findings provide quantitative histological support for the localized tissue effects of plasma-based devices and their rationale in aesthetic procedures. Full article

Endometrial carcinoma (EC) is now classified primarily by molecular subtype—POLE-ultramutated, mismatch repair–deficient (dMMR), TP53-mutant/copy-number-high (CNH), and “no specific molecular profile” (NSMP)—a framework that has reshaped prognostic counseling and adjuvant therapy decisions. Yet several practically important questions remain insufficiently addressed in real-world cohorts: whether all four mismatch repair genes confer an equivalent favorable prognosis, whether all POLE alterations carry the same survival benefit or only specific pathogenic variants, and whether molecular subtypes retain prognostic value after adjustment for histology and tumor burden. We aimed to address these questions in 2901 patients pooled from the MSK-IMPACT 50K Clinical Sequencing Cohort (n = 2372; discovery) and the TCGA UCEC PanCancer Atlas (n = 529; validation)—the largest dual-cohort genomic analysis of EC reported to date. Across both cohorts, all four MMR gene–mutant subgroups (MLH1, MSH2, MSH6, PMS2) conferred equivalently favorable overall survival (OS) (six-group log-rank p = 7.66 × 10⁻¹² in discovery; p = 6.78 × 10⁻³ in validation), confirming dMMR as a class-level prognostic designation independent of which MMR gene is altered. Multivariable Cox regression demonstrated that POLE-ultramutated status retained an independent favorable effect (HR = 0.62, p = 0.038 in MSK; HR = 0.35, p = 0.028 in TCGA) after adjustment for age, histology, and sample type, while the favorable dMMR effect was largely accounted for by histologic context. Critically, a pathogenicity-aware sensitivity analysis revealed that the exceptional survival of the POLE subgroup is confined to canonical exonuclease-domain hotspot mutations (event rate 0.9% in MSK), whereas POLE variants of uncertain significance behave indistinguishably from NSMP-like tumors. Consistent with this finding, tumor mutational burden (TMB) was markedly elevated in canonical pathogenic POLE cases (median 138.7 mut/Mb in MSK; 247.4 in TCGA) but not in POLE-VUS-only cases (median 29.0 and 15.0, respectively; p < 0.001 between groups in both cohorts), confirming that the ultramutator phenotype is confined to canonical pathogenic POLE variants. We additionally characterize Uterine Clear Cell Carcinoma as a distinct histologic entity (n = 73; 3.0%) and report the POLE + TP53 co-mutant group (n = 90; 3.8%). Together, these findings refine the molecular classification of EC in clinically meaningful ways: they support class-level immunotherapy eligibility based on dMMR status regardless of the specific MMR gene altered, demonstrate that POLE-ultramutated classification requires variant-level pathogenicity assessment, and identify TP53-mutant/CNH patients as the population with the most urgent unmet therapeutic need. Full article

To reveal the mechanisms by which obstacle distribution affects propane–air premixed deflagration under different blockage ratios, large eddy simulation (LES) was employed to investigate flame propagation and pressure rise in a confined duct with four obstacle distributions and four blockage ratios. The coupled effects of obstacle layout and blockage ratio on flame morphology, propagation velocity, vorticity evolution, and pressure rise rate were analyzed. The results show that obstacle distribution significantly changes flame front structures: One-side obstacles produce claw-like flames, center layout obstacles generate tongue-like flames with large vortex regions at low-to-moderate blockage ratios, and both-side or around layout obstacles form mushroom-like flames. At high blockage ratios, around layout obstacles redirect the flow into a high-speed axial jet, leading to the highest flame velocity and maximum pressure rise rate. These findings indicate that the dominant flame acceleration mechanism shifts from vortex-induced flame wrinkling at low-to-moderate blockage ratios to axial-jet-driven flame acceleration at high blockage ratios, providing guidance for obstacle layout optimization and explosion risk mitigation in confined propane–air systems. Full article

Glass fiber-reinforced polymer (GFRP) composites offer a compelling solution to the durability degradation of reinforced concrete (RC) structures in harsh marine and de-icing environments. Hybridizing fiber-reinforced polymer (FRP) with conventional steel reinforcement synergizes the superior corrosion resistance of FRP with the high ductility of steel. However, the synergistic mechanisms of GFRP–steel hybrid reinforced columns confined by either GFRP or steel spiral stirrups under axial compression remain insufficiently quantified. This study systematically investigates the axial compressive performance of such structures through material testing, static axial compression tests on seven short column specimens, and advanced finite element (FE) modeling. The investigation focuses on the effects of the steel-to-GFRP area ratio and the spiral stirrup type. Experimental results reveal that spirally confined hybrid columns exhibit failure modes remarkably similar to conventional RC columns. The incorporation of GFRP bars significantly enhanced the ultimate load-bearing capacity, while the steel bars ensured the requisite ductility. Notably, a higher ultimate capacity was achieved at a steel-to-GFRP area ratio of 1:1 under steel spiral confinement, retaining a ductility index equivalent to 83.6% of a pure RC column. Furthermore, an ABAQUS-based FE model was developed and rigorously validated against experimental data, successfully capturing the failure progression and ultimate capacities across diverse parameters. Ultimately, based on the superposition principle, by quantifying the independent load-bearing contributions and synergistic interactions of the spalled concrete cover, confined core, and hybrid bars, this study derives a theoretical formula. The proposed model accurately predicts the axial compressive capacity of spirally confined hybrid columns, providing an analytical tool for resilient structural design. Full article

This study investigates the effects of flow distributor placement and loosener configuration on particle-flow behavior in a hydrogen-based direct reduction shaft furnace using the discrete element method (DEM). A three-dimensional industrial-scale furnace model based on a MIDREX-type geometry was established, and four representative structural configurations were examined by varying the flow distributor position and loosener setting. The results show that flow distributor placement is the dominant factor controlling particle descending behavior and particle-flow uniformity. When the flow distributor was located in the cooling zone, the flow uniformity index reached 0.875, which was 40.9% and 20.9% higher than those for the transition–cooling interface and transition-zone configurations, respectively. Particle trajectory analysis indicates that the effect of flow distributor position is mainly confined to the region above the device, with limited influence on the lower burden trajectory. Although the loosener has little effect on particle-flow uniformity, it significantly suppresses particle degradation. Under the transition-zone flow distributor configuration, the predicted powder formation ratio decreased from 3.89% to 2.97% after introducing the loosener, corresponding to a relative reduction of 23.7%. Overall, among the four representative configurations investigated in this study, positioning the flow distributor in the transition zone while retaining the loosener provides a more balanced compromise between burden-flow regulation and powder suppression for shaft furnace design and industrial operation. Full article

p-nitrophenol (p-NP) is a pollutant with environmental persistence, bioaccumulation potential, and significant health risks, and is widely dispersed in wastewater, so efficient removal of p-NP is imperative. Among the various methods, the catalytic reduction of p-NP to p-aminophenol (p-AP) using sodium borohydride (NaBH₄) is a particularly promising one and, herein, catalysts play a crucial role. Among the various metals, Au shows unique catalytic activity for p-NP reduction. However, nanosized Au often exhibit limited activity and stability due to their high surface free energy. To address this challenge, we designed and synthesized Au-SnO_x hybrid nanoparticles confined within hollow mesoporous silica nanoreactors (Au-SnO_x@hm-SiO₂) via a soft-template-assisted co-adsorption strategy. The resulting bimetallic Au-SnO_x@hm-SiO₂ nanoreactor showed significantly enhanced catalytic activity toward the NaBH₄-mediated reduction of p-nitrophenol (p-NP) compared with its monometallic Au@hm-SiO₂ counterpart, owing to the synergistic effect between Au and SnO_x. Among various Au/Sn ratios, the catalyst with an Au/Sn molar ratio of 1:0.1 demonstrated the highest activity, achieving complete conversion of p-NP within 5 min at a p-NP/Au molar ratio of 529:1—a tenfold improvement over Au@hm-SiO₂. Moreover, the catalyst maintained high efficiency over six consecutive cycles, with only slight deactivation, benefiting from the protective silica shell. Full article

This study systematically investigated flow boiling characteristics within a novel three-layer microchannel heat sink with 3/4 open-ring pin fin arrays, designed for high-heat-flux thermal management of low-carbon metallurgical reactors. Two-phase flow regimes, pressure drop, and wall temperature responses were analyzed. To evaluate the impact of functional surface material properties on thermo-hydraulic behavior, a hydrophilic nano-coating modification was applied to the inner copper channel walls for comparison. Increasing the flow rate triggered a transition from a vapor-dominated confined slug flow to a liquid-dominated dispersed bubble flow, which effectively improved the thermo-hydraulic stability. Hydrophilic surface modification resulted in an average pressure drop reduction of 33% and significantly diminished the sensitivity of flow resistance to velocity variations. Through hydrophilic treatment, the localized vapor film effect at high velocities was suppressed, and temperature field homogenization was promoted, yielding a maximum convective heat transfer coefficient of 7760 W/(m²·°C), i.e., 72.9% enhancement over the baseline heat sink. The underlying mechanism is attributed to the formation of a stable near-wall thin liquid film and the promotion of high-frequency nucleate boiling. These results will be of high relevance for developing efficient cooling solutions for power electronics, thereby supporting the advancement of low-carbon metallurgical reactors. Full article

This study presents the development and validation of a simulation-calibrated kinematic formulation for a three-wheeled symmetric pipeline inspection robot operating under cylindrical confinement. The proposed model integrates analytical implementation in MATLAB 2023b with multibody simulation in SolidWorks 2023 to identify semi-empirical correction terms that improve motion prediction under straight and curved pipe conditions. The formulation incorporates curvature-dependent and asymmetry-related effects derived from structured simulation datasets, ensuring consistency between analytical predictions and simulated behavior within the evaluated operating range. Quantitative comparison using statistical indicators demonstrates strong agreement between both approaches, with MAE values of 0.0547 for linear velocity and 13.96 for displacement, RMSE values of 0.0681 and 19.0401, and coefficients of determination of $R^2=0.9997$ and $R^2=0.9476$, respectively. Slightly larger deviations are observed at higher rotational speeds. The results provide a consistent analytical representation of the robot’s motion under the studied geometric constraints and establish a basis for future experimental validation and control-oriented extensions in confined pipeline environments. Full article

The rehabilitation of the loggerhead sea turtle, Caretta caretta, involves stressors like handling and confinement. To assess physiological stress responses during rehabilitation, twenty-five C. caretta hospitalized at C.Re.Ta.M. were monitored over a two-month period at three time points (T0, T1, and T2). The cohort included 12 juveniles (CCL: 30.6 ± 5.7 cm) and 13 subadults (CCL: 52.5 ± 10.4 cm). Heterophil/lymphocyte ratios (H/L), corticosterone (CORT), glucose (Glu), creatine kinase (CK), and uric acid (UA) plasma concentrations were assessed. Two-way repeated-measure ANOVA revealed significant time effects on H/L ratio (p < 0.0001), CORT (p < 0.0001), Glu (p = 0.0002), CK (p < 0.0001), and UA (p < 0.05), with a significative group x time interaction observed for CK (p = 0.016), CORT (p = 0.006) and UA (p = 0.035). No group effect was observed in any of the data. In the juvenile group, H/L (p < 0.01) and CORT (p < 0.001) were significantly lower at T2 compared to T0. At the T0 point, CORT levels were significantly higher in juveniles compared to the subadult group. In subadults, significant decreases in H/L ratio (p < 0.001), Glu (p < 0.01), CK (p < 0.001), and UA (p < 0.05) were observed at both T1 and T2 relative to T0. At T0, CK levels were significantly higher in subadults compared to juveniles. No significant correlations were found between CORT and the other measured parameters. Our results suggest that the rehabilitation period is a safety period during which the animals reestablish their homeostasis despite captivity conditions. However, further studies are needed to define other causes of variations in stress levels in rehabilitating C. caretta. Full article

Accurate prediction of Rate of Penetration (ROP) in carbonate formations remains constrained by the arbitrary selection of geomechanical input parameters in empirical drilling models. This study presents the first systematic field-based evaluation of sixteen geomechanical properties—grouped into three categories: strength parameters (uniaxial compressive strength (UCS), confined compressive strength (CCS), shear strength, thick-walled cylinder strength (TWC), friction angle, and cohesion), elastic moduli (Young’s modulus, shear modulus, bulk modulus, bulk compressibility, dynamic combined modulus (DCM), Poisson’s ratio, brittleness index), and in situ stress parameters (overburden pressure, minimum, and maximum horizontal stresses)—to identify optimal predictors for ROP modeling across PDC bit sizes of 12.25″ and 8.5″. Continuous wireline log data from two vertical carbonate wells in the Middle East (Well A: 1000–3370 m; Well B: 1945 to 3128 m; total intervals of 2370 m and 1183 m, respectively) penetrating formations comprising limestone, dolomite, sandstone, shale, anhydrite, and marly limestone were used. All sixteen geomechanical properties were computed using Interactive Petrophysics (IP) software with lithology-specific empirical correlations and validated against laboratory core measurements (R² = 0.79–0.95). Pearson and Spearman correlation analyses quantified parameter–ROP relationships, and the Al-Abduljabbar empirical model, recalibrated via multiple nonlinear regression, served as the evaluation framework. DCM consistently exhibited the strongest negative correlation with ROP across both bit sizes and achieved the highest model accuracy (R² = 0.54, AAPE = 25.33%), significantly outperforming the Bourgoyne and Young model (R² = 0.26, AAPE = 36.55%). A statistically validated scale-dependent effect was identified: Fisher’s Z-transformation tests confirmed that the correlation reversal between CCS and UCS across bit sizes is statistically significant (CCS: Z = −16.84, p < 0.001; UCS: Z = −6.75, p < 0.001), establishing CCS as the superior predictor at 12.25″ and UCS as the superior predictor at 8.5″—a finding not previously reported in the ROP literature. This reversal is attributed to the larger contact area of the 12.25″ bit, which promotes confinement-dominated rock failure better described by CCS, whereas the smaller bit produces localized stress concentration better represented by UCS. These results establish that (1) optimal geomechanical input selection is bit-size dependent, (2) nonlinear modeling outperforms linear frameworks for strength–ROP relationships, and (3) parameter relevance outweighs coefficient tuning in model robustness. DCM is recommended as the most operationally practical universal input, requiring only conventional compressional sonic and density logs. This study provides a systematic framework for geomechanical parameter selection with direct implications for drilling optimization in heterogeneous carbonate reservoirs. Full article

Rare-earth-doped microsphere cavities have attracted significant interest for applications in miniaturized photonic devices due to their unique optical properties. In this work, Yb³⁺/Er³⁺ co-doped microsphere cavities were fabricated via a melting method, which enables uniform interior doping at high and tunable rare-earth concentrations through a simpler and more cost-effective process compared with existing coating and fiber-etching approaches. Whispering gallery modes (WGMs) enhanced upconversion luminescence, which was observed using tapered fiber coupling, producing a vivid green fluorescence ring near the equatorial region of the microsphere. The luminescence characteristics of the microsphere cavity were investigated by measuring the fluorescence spectra under varying excitation powers. The results indicated that the fluorescence emission follows a two-photon absorption process, consistent with the upconversion emission mechanism of Er³⁺. A finite difference time domain (FDTD) model was employed to simulate the optical field distribution within the microsphere cavity. At a microsphere diameter of 90 μm and a coupling gap of 0 μm, both the 980 nm pump light and the emitted light were effectively confined near the equatorial region of the microsphere, forming WGM confinement patterns. These findings are expected to advance the application of rare-earth-doped microsphere cavities in fields such as biosensing, bioimaging, optical communications, and upconversion microlasers. Full article

Background/Objectives: Allomyrinasin is a cationic antimicrobial peptide derived from Allomyrina dichotoma larvae with known antibacterial and anti-inflammatory properties; however, its effects on migration-related mechanisms in oral squamous cell carcinoma (OSCC) remain poorly understood. This study investigated the anti-migratory potential of allomyrinasin in OSCC cells, focusing on Na⁺/HCO₃⁻ cotransporter (NBC) activity as a key migratory module. Methods: NBC activity was assessed in YD-38 OSCC cells treated with allomyrinasin. Cell migration was evaluated by wound healing and Transwell assays, and MMP expression. Intracellular reactive oxygen species (ROS), apoptosis-related markers, and lamin A/C expression were analyzed using fluorescence-based assays and gene expression analysis. Results: Allomyrinasin inhibited NBC activity and suppressed cell migration without substantial loss of cell viability. MMP-13 was selectively downregulated among the tested MMPs. Lamin A/C expression was markedly upregulated, suggesting enhanced nuclear stiffness that may restrict confined cell migration. Intracellular ROS levels were elevated, and apoptotic progression was confirmed by increased Annexin V/PI positivity along with downregulation of B-cell lymphoma 2 (BCL2) and upregulation of BCL-2–associated X genes (BAX), through a p53-independent pathway consistent with the TP53-deleted status of YD-38 cells. Conclusions: Allomyrinasin suppresses OSCC cell migration by targeting NBC activity as a key component of the migratory machinery, accompanied by oxidative stress induction and pro-apoptotic signaling. These findings identify allomyrinasin as a potential anti-migratory therapeutic candidate and highlight NBC activity as a promising target for attenuating cancer metastasis. Full article

This study presents a mechanics based analytical framework for predicting the flexural–shear capacity of reinforced concrete (RC) beams strengthened with Engineered Cementitious Composites (ECCs) and a hybrid ECC–GFRP near surface mounted (NSM) system. Building upon previously reported experimental observations, the present work aims to establish rational prediction models capable of capturing the interaction between flexural and shear mechanisms in strengthened beams. The analytical approach integrates sectional analysis for flexural capacity with a modified truss analogy for shear resistance, explicitly incorporating the strain hardening tensile contribution of ECC and the tensile and confinement effects of GFRP reinforcement. An interaction based failure criterion is subsequently employed to identify the governing failure mode under combined flexural shear actions. The proposed model is validated against experimental results obtained from twenty seven beam specimens with varying flexural and shear reinforcement ratios and strengthening configurations. The predicted ultimate loads show good agreement with experimental values, with an average deviation within ±10%. The analytical framework accurately captures the transition between flexural dominated, combined flexural–shear, and diagonal tension failures observed experimentally. Results demonstrate that ECC significantly enhances ductility and shear crack control, while the hybrid ECC–GFRP system provides substantial strength enhancement with a controlled shift in failure mode. Overall, the developed analytical models offer a reliable and computationally efficient tool for predicting the flexural–shear capacity and failure behavior of ECC and hybrid ECC–GFRP-strengthened RC beams, supporting performance based design and practical strengthening applications. Full article

Multifunctional polymer–ferrite composites based on poly(vinylidene fluoride) (PVDF) and magnetic fillers are of increasing interest for applications requiring coupled electrical, dielectric, and magnetic responses. However, the relationship between magnetic filler concentration, PVDF phase composition, and the resulting multifunctional properties remains insufficiently understood. In this work, PVDF/BaFe₁₂O₁₉ (PVDF/BaF) composite membranes containing 2–20 wt.% BaF were fabricated using a combined non-solvent and thermally induced phase-inversion (NIPS–TIPS) method. Structural evolution was analyzed by X-ray diffraction and quantitative FTIR spectroscopy, thermal behavior by differential scanning calorimetry, optical properties by diffuse reflectance spectroscopy, dielectric response in the frequency range 10³–10⁶ Hz, and magnetic characteristics by vibrating sample magnetometry. At moderate filler concentrations (2–10 wt.%), BaFe₁₂O₁₉ nanoparticles acted as effective β-phase nucleating centers, leading to electroactive phase fractions of 97.7–99.9% and a maximum β-phase content of 86.7% for PVDF/BaF10. At higher loadings (15–20 wt.%), particle agglomeration and restricted chain mobility promoted a transition toward α-phase-dominated crystallization. Thermal analysis indicated competing nucleation and confined crystallization processes, while optical and dielectric measurements revealed nonmonotonic changes associated with interfacial interactions and Maxwell–Wagner–Sillars polarization. Magnetic measurements showed a linear increase in saturation magnetization with filler concentration and a nonmonotonic coercivity dependence with a pronounced change near the critical agglomeration concentration. These results demonstrate that the multifunctional response of PVDF/BaFe₁₂O₁₉ membranes is governed by the interplay between β-phase nucleation, interfacial polarization, and magnetic particle interactions, with approximately 10 wt.% ferrite providing the most balanced electrical, dielectric, and magnetic characteristics. Full article

Polyacrylamide (PAM), as a widely used effective soil conditioner, can decrease sandy soil infiltration, but its function may decline significantly in a short time. Previous study results showed that the annual degradation rate of PAM in soil is about 10%, and the migration ability of PAM in soil is fairly weak; thus we hypothesized that the functional group of PAM is prone to aging caused by physical and biological factors, which is different from degradation caused by the breaking of long main chains into short ones. The sandy loam soil was selected to conduct column infiltration experiments to (1) determine the effects of PAM application and drying and wetting intensity on infiltration and (2) identify the causes of the aging effect. Soil samples were treated with three doses of PAM (0, 1, and 2 g·kg⁻¹) and incubated in three soil water conditions (constant wetting, moderate and strong drying/wetting cycles). Under constant wetting condition, the stable infiltration rates of soils were decreased by PAM. However, after two strong drying and wetting cycles, the decrement of infiltration rates of PAM-treated soils was reversed. The results of FTIR suggested that drying and wetting cycles led to the hydrolysis of amide groups in PAM, resulting in the weakening of PAM’s function on soil infiltration characteristics. The leaching amounts of NH₄⁺-N generated by the amide group hydrolysis increased through the drying/wetting alternation and the application of PAM. Therefore, based on the findings of this column study using a specific sandy loam soil under controlled intense drying–wetting cycles, reapplication of polyacrylamide (PAM) after two cycles may facilitate the sustained lowering of infiltration. However, this recommendation should be confined to analogous experimental conditions and necessitates further validation under field scenarios or for alternative soil types. Full article