Search Results (61)

: This study reports the development of a sustainable biocatalyst system for free fatty acid (FFA) production from cocoa bean shell (CBS) oil using Burkholderia cepacia lipase (BCL). CBS was explored as both a support material and a reaction substrate. Six immobilized systems were prepared using organic (CBS), inorganic (silica), and hybrid (CBS–silica) supports via physical adsorption or covalent binding. Among them, the covalently immobilized enzyme on CBS (ORG-CB) showed the most balanced performance, achieving a catalytic efficiency (Ke) of 0.063 mM⁻¹·min⁻¹ (18.6% of the free enzyme), broad pH–temperature tolerance, and over 50% activity retention after eight reuse cycles. Thermodynamic analysis confirmed enhanced thermal resistance for ORG-CB (Ed = 32.3 kJ mol⁻¹; ΔH‡ = 29.7 kJ mol⁻¹), while kinetic evaluation revealed that its thermal deactivation occurred faster than for the free enzyme under prolonged heating. In application trials, ORG-CB reached 60.1% FFA conversion from CBS oil, outperforming the free enzyme (49.9%). These findings validate CBS as a dual-function material for enzyme immobilization and valorization of agro-industrial waste. The results also reinforce the impact of immobilization chemistry and support composition on the operational and thermal performance of biocatalysts, contributing to the advancement of green chemistry strategies in enzyme-based processing. Full article

Coastal regions of Ecuador, particularly Esmeraldas and Manabí, face significant challenges related to energy access, waste management, and sustainable agricultural development. This study evaluates the renewable energy potential of cocoa waste biomass generated by smallholder farms in these provinces. A total of 20 cocoa farms, either certified or in the process of certification under the Rainforest Alliance standard, were surveyed to quantify the volume of agricultural and agro-industrial residues. Residual biomass generation ranged from 50 to 6500 tons per year, depending on farm size, planting density, and management practices. Spatial analysis revealed that Esmeraldas holds the highest concentration of cocoa waste biomass, with some farms reaching a gross energy potential of up to 89.07 TJ/year. Using thermochemical conversion scenarios, effective energy potential was estimated, and 75% of the farms exceeded the viability threshold of 100 MWh/year. The results confirm the feasibility of cocoa biomass as a renewable energy source, mainly when managed collectively at the community level. Incorporating this waste into decentralized energy systems supports circular economy models, enhances energy self-sufficiency, and aligns with sustainable supply chain goals promoted by certification schemes. This study contributes to national efforts in energy diversification and provides a replicable model for integrating renewable energy into rural agricultural systems. Full article

This paper studies the potential of utilizing cocoa residues to address energy demands in post-conflict regions of Colombia. Through a systematic review, the findings indicate that cocoa byproducts from 170 municipalities could produce enough biogas to power a population nearing one million inhabitants. Three main residues can be obtained from processing cocoa—mucilage, cocoa bean shells, and cocoa pod husks—which are analyzed and recognized as the most significant in terms of potential biogas yield. The review highlights a considerable gap in existing research regarding the specific characteristics of cocoa residues and their biogas production rates. Additionally, this study emphasizes the need to investigate pretreatment methods for these materials, particularly cocoa pod husks, as well as the critical impact of transportation logistics. It also suggests exploring integrated valorization strategies and various energy conversion pathways. Finally, the article emphasizes the importance of establishing markets and promoting new initiatives to effectively utilize this abundant amount of waste. The insights gained from this analysis are relevant to similar regions, not only across South America but also in any cocoa-producing area worldwide. Full article

Cacao mucilage is a rich medium for microbial development due to the presence of various sugars, water, pectin, mineral salts, and yeasts of the Saccharomyces cerevisiae type. This study aims to provide added value to this commonly discarded residue, thereby contributing to the economic growth of the Rio Chila area in the Valencia Canton of Los Ríos Province. The methods applied for developing beer consist of malting, grinding, mashing, filtering, boiling, cooling, fermentation (during which cacao mucilage is added), and maturation, followed by physical–chemical analyses. The Fine aroma cacao mucilage presented values of 0.66% acidity, 7.63 °Brix, pH 4.43, absorbance 1.13, transmittance 23.67%, suspended solids 0.04 g: 2.66%, density 1.07 g/mL, turbidity 6.94 NTU, °GL 8.47% vol., foam quantity 1.70 cm, colorimetry L* 50.77, colorimetry a* 18.08, colorimetry b* 50.53, and bitterness degree 39.00. The analyses presented values within the normal parameters applied to beers at the national level (INEN standards). Escherichia coli, Salmonella, and total microorganisms showed no contamination in the microbiological analyses. In the sensory analyses, appearance, aroma, flavour, and mouthfeel were evaluated, with the best experiment being the combination of Fine aroma cacao with a concentration of 30% mucilage and added Cascade hops. This study took into account the concentrations of cacao mucilage (20% and 30%) from the varieties (Fine aroma and CCN-51), as well as the addition of the brewing hops Cascade and Northern Brewer. Regarding the physicochemical characteristics, adding this cacao derivative did not affect craft beer and conformed to the ranges of the NTE INEN 2262 standard. Thus, this research proposes an alternative use for cocoa mucilage, contributing to waste reduction and broadening its potential applications. Full article

Cocoa pod husk (CPH), a significant agricultural byproduct of cocoa production, presents an opportunity for sustainable valorization through biotechnological methods. This study aimed to enhance the nutritional, antioxidant, and therapeutic properties of CPH using solid-state fermentation (SSF) with Rhizopus stolonifer. Physicochemical characterization confirmed CPH’s suitability for SSF, providing a nutrient-rich and favorable environment for fungal growth. The fermentation process significantly improved protein recovery (11.327 ± 0.859 mg g⁻¹) and antioxidant activity, with ORAC (51.68 ± 0.35 mmol TE g⁻¹) and DPPH (7.09 ± 0.05 µmol TE g⁻¹) assays demonstrating marked increases in redox potential, particularly at 144 h and 96 h of fermentation, respectively. GC-MS analysis revealed the generation of bioactive compounds in fermented CPH (CPHF), including methyl 3-hydroxybutyrate, 10,12-Tricosadiynoic acid, and palmitic acid, which are known for their antioxidant, anti-inflammatory, and therapeutic properties. Additionally, phenolic compounds are biotransformed into more bioavailable forms, further enhancing the functional value of the material. This work demonstrates that SSF can effectively transform CPH from an agricultural waste product into a high-value biomaterial with potential applications in functional food, nutraceutical, and pharmaceutical industries. By addressing waste management challenges and promoting the development of innovative bio-based products, this study highlights the promising role of SSF in advancing sustainable and circular biotechnological solutions. Full article

The Cocoa Pod Husk (CPH) accounts for 67–76% of the total cocoa fruit weight, making it its main agro-industrial waste of cocoa production. A valorization of this waste is possible through the extraction of pectin. In this study, pectin was extracted from CPH powder by acid hydrolysis using citric acid and sulfuric acid. Fourier transform infrared spectroscopy (FT-IR) was employed as a qualitative and quantitative characterization technique. The FT-IR of the pectin samples showed the bands visible at 1732 and 1626 cm⁻¹ corresponding to the esterified and free carboxylic groups, respectively. These bands can be differentiated according to their degree of methyl esterification (DE) by analyzing the area under the curve. The extracted pectin showed no significant difference in yields (p ≤ 0.05) between the two acids; however, significant differences (p ≤ 0.05) were observed in DE and methoxylation percentage (MeO). According to the FT-IR results, pectin extracted with citric acid presented a lower DE (7.43%) and MeO (1.12%) compared to pectin extracted with sulfuric acid, which showed a DE of 18.15% and a 2.96% MeO. Pectin with a DE below 50% is classified as low-methylated, making it unsuitable for the food industry. However, these create a raw material that has a potential use in the pharmaceutical and bioenergy industries. Full article

The increasing generation of agro-industrial waste has intensified soil and water contamination, as well as the eutrophication of water bodies, impacting biodiversity and human health. This highlights the need for responsible management to meet Sustainable Development Goals (SDGs) 3, 6, 12, 13, 14, and 15, which promote health, access to clean water, responsible consumption, climate action, and the protection of life on land and below water. This study aimed to produce activated carbon from cocoa, baru, and monguba residues for the removal of contaminants dyes (methylene blue, bromocresol green, and methyl red) presented in wastewater. The three materials were carbonized at 500 °C for one hour under a nitrogen atmosphere and activated with H₃PO₄. The samples were characterized using TGA, SEM, XRD, FT-IR, pH_PZC, and ASAP, in addition to conducting kinetic and thermodynamic parameter assays for the dyes. Monguba carbon exhibited the highest pore volume (1.57 cm³·g⁻¹), surface area (1604 m²·g⁻¹), and adsorption capacity for methylene blue and methyl red (50 mg·g⁻¹). The data were analyzed using pseudo-first and pseudo-second order kinetic models. It was concluded that monguba carbon shows potential for the sustainable removal of organic dyes and molecules with similar characteristics in contaminated water or wastewater. Full article

This work describes the development of a method for the extraction of methylxanthines from cocoa bean shell (CBS) by employing the novel Energized Dispersive Guided Extraction (EDGE) system. The mixtures were composed of ethanol–methanol–water and the ratio was optimized using a simplex-centroid design. Doehlert design (DD) was used to optimize the variables of temperature and time while using methylxanthine content obtained by HPLC-DAD as an analytical response. The optimized mixture consisted of water–ethanol in a 3:2 ratio. The optimum operating conditions for extraction were achieved at a temperature of 148.5 °C and 382 s. Under optimal conditions, 20.14 mg g⁻¹ DM of theobromine and 3.53 mg g⁻¹ DM of caffeine were found in the CBS extract. Methylxanthines were quantified with good linearity, LOQs, LODs, precision, and accuracy. The EDGE system, a newly automated extraction instrument, has proven to be very efficient for the recovery of theobromine and caffeine, and is considered a green extraction procedure, as demonstrated by the analytical greenness metric for sample preparation. Full article

Cocoa shells (variety CCN51, exclusive from Ecuador) were subjected to different thermochemical processes (combustion, pyrolysis, and gasification) to evaluate their potential for energy production. Pyrolysis was conducted at 500, 600, and 700 °C, yielding solid (35.89–41.27%), liquid (31.13–34.73%), and gas (24.92–32.92%) fractions. The higher heating value (HHV) of the solid phase increased with temperature, reaching 24.97 MJ/kg at 700 °C. Gasification was performed under air flow at 500, 600, and 700 °C, producing synthesis gas with an HHV ranging from 0.89 to 3.36 MJ/m³. The H₂ and CO contents in the gas phase increased with temperature, reaching 9.98% and 11.77% at 700 °C, respectively. Combustion analysis, conducted via thermogravimetry coupled with mass spectrometry, revealed efficient volatile release and oxidation patterns. The high potassium content in the ashes (72.02% K₂O) may affect slagging behavior but also presents opportunities for agricultural applications. These findings highlight the potential of CCN51 cocoa shells as a sustainable biomass resource, contributing to Ecuador’s green transition and the local circular economy. Full article

Coffee and cocoa agribusinesses generate large volumes of byproducts, including coffee husk, coffee pulp, parchment skin, silver skin, and cocoa bean shell. Despite the rich composition of these materials, studies on biomolecule extraction with green solvents are still scarce, and further research is needed. Extraction methods using alternative solvents to obtain biomolecules must be developed to enhance the byproducts’ value and align with biorefinery concepts. This article reviews the compositions of coffee and cocoa byproducts, their potential applications, and biomolecule extraction methods, focusing on alternative solvents. The extraction methods currently studied include microwave-assisted, ultrasound-assisted, pulsed electric field-assisted, supercritical fluid, and pressurized liquid extraction. At the same time, the alternative solvents encompass the biobased ones, supercritical fluids, supramolecular, ionic liquids, and eutectic solvents. Considering the biomolecule caffeine, using alternative solvents such as pressurized ethanol, supercritical carbon dioxide, ionic liquids, and supramolecular solvents resulted in extraction yields of 2.5 to 3.3, 4.7, 5.1, and 1.1 times higher than conventional solvents. Similarly, natural deep eutectic solvents led to a chlorogenic acid extraction yield 84 times higher than water. The results of this research provide a basis for the development of environmentally friendly and efficient biomolecule extraction methods, improving the utilization of agricultural waste. Full article

This study evaluated the desorption of cadmium (Cd) from cocoa waste-derived flour using organic acids. Cocoa pods were collected from Antioquia and Casanare, Colombia, to analyze the geographical Cd content and its distribution within the pod tissues. Acid selection was performed using a multi-criteria decision-making (MCDM) matrix, and Cd desorption was assessed through a full factorial 2³ experimental design, considering acid concentration, pulp density, and agitation speed. Additionally, the oxidation–reduction potential (ORP) was monitored as an indicator of the electrochemical dynamics of the process. The results indicated that pods from Casanare exhibited higher Cd concentrations (1.63 ± 0.20 ppm) compared with those from Antioquia (0.87 ± 0.22 ppm), with 49.31% of the metal being accumulated in the pod. Parameters of citric acid at 0.5 M, 5 g/L pulp density, and 120 rpm were found to be optimal for the Cd desorption process, achieving over 95% efficiency. Based on ORP monitoring, a heuristic was proposed to determine the contact time during leaching. This work outlines a scalable process for Cd desorption, adding value to cocoa industry waste for potential applications. Full article

To mitigate the emissions of greenhouse gases (GHGs) from fossil fuels, the use of biodiesel and its sustainable production have been receiving more attention over the past decade, especially for the reuse of waste cooking oils and non-edible oils as starting feedstocks. For the biodiesel production process, the suitability of a green catalyst is a core function in the transesterification reaction. Heterogeneous (solid-state) catalysts are generally superior to homogeneous (liquid-state) catalysts due to several significant advantages such as no saponification products formed, recyclability, and less equipment corrosion. Recent studies also revealed that heterogeneous solid base catalysts were widely used for the production of biodiesel. Furthermore, the use of biomass-based ash derived from herbaceous and agricultural biomass is increasing rapidly because of its environmental sustainability, high biodiesel yield, and low catalyst cost. To highlight alternative catalysts from biomass residues, this mini-review paper thus focused on a summary of various heterogeneous potassium-rich ash materials, which were used as green catalysts for the sustainable production of biodiesel. Due to the abundant quantity and chemical compositions, it was found that ash derived from cocoa pod husk may be the most commonly used solid base catalyst for producing biodiesel in the literature. Finally, future perspectives on biodiesel production by adopting emerging technologies and using high-potassium (K) biomass ash as a green catalyst were also addressed. Full article

The increase in food production is accompanied by an increase in waste, particularly agricultural by-products from cultivation and processing. These residues are referred to as agricultural by-products. To address this issue, biotechnological processes can be used to create new applications for these by-products. This study explored the use of LAB strains (Lactiplantibacillus plantarum, Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Limosilactobacillus fermentum) on by-products such as white grape pomace, cocoa bean shells, apple pomace, and defatted roasted hazelnut to develop yoghurt-style fruit beverages. Microbial load and pH changes were monitored during a 24 h fermentation and 14-day shelf life at 5 °C. Concentrations of sugars, organic acids, and volatile organic compounds were also analyzed using HPLC and GC-qMS. The results showed that optimizing the matrix led to significant bacterial growth, with viable microbes remaining under refrigeration. In particular, the strain of L. plantarum tested on the cocoa bean shell yielded the most promising results. After 24 h of fermentation, the strain reached a charge of 9.3 Log CFU/mL, acidifying the substrate to 3.9 and producing 19.00 g/100 g of lactic acid. Aromatic compounds were produced in all trials, without off-flavours, and characteristic fermented food flavours developed. Additionally, secondary metabolites produced by lactic acid bacteria may enhance the health benefits of these beverages. Full article

The demand for innovative products from renewable sources has motivated research development to create new sustainable materials. Cassava starch (CS) has been widely used for bonding and composing different types of products. Particleboards produced from cocoa (Theobroma cacao), wood wastes, and CS adhesives can be an environmentally correct and economically profitable alternative to replacing traditional commercial panels. This study aimed to manufacture particleboards made with wood waste extracted from the stem of Theobroma cacao. The panels were bonded with different proportions of CS and urea–formaldehyde (UF) adhesives, and their physical–mechanical properties were determined. To manufacture the panels, cocoa wood wastes were mixed with the adhesive in ratios of 90:10, 70:30, and 50%:50% (CS/UF). Two control treatments were bonded with 100% of both adhesives. The resulting particleboards were employed as a reference to compare properties. The manufacturing process was carried out by cold pressing. Apparent density, water absorption, thickness swelling, and static bending strength were found for all panels. The data obtained were subjected to Levene’s homogeneity test, Shapiro–Wilk’s normality test, analysis of variance (ANOVA), and Tukey’s mean test. The results showed that the highest density value was 497.0 kg m⁻³, corresponding to the treatment composed of cocoa wood wastes bonded with 100% CS adhesive. The water absorption and thickness swelling results after a 24 h immersion showed that the panel formed using cocoa wood wastes and 100% UF had the lowest values, 22.1 and 11.2%. The highest bending strength value was 13.1 MPa for the experimental treatment composed of cocoa wood residue and 100% UF. However, this result did not differ statistically from the treatment (50–50). Therefore, cocoa wood waste combined with adhesive CS may be a sustainable alternative for producing particleboards. Full article

Cocoa production has emerged as an effective agricultural strategy to reduce conflict in Colombia, transitioning from coca to cocoa cultivation. While this shift has provided economic benefits, it has also resulted in the generation of substantial cocoa by-products. Although there are various alternative methods of utilizing these by-products, many farmers are unaware of them, and others lack the necessary tools to determine which alternative is the best to pursue. This study sought to explore sustainable options for cocoa waste utilization through the application of the Analytic Hierarchy Process (AHP). By employing technological surveillance, viable options for reusing cocoa residues were identified. The AHP results indicate that pellet production is a promising alternative for rural communities. It is also a potential source of energy that could address the community’s need for alternative energy sources. Initially, other energy production alternatives were not explored. However, in response to the AHP findings, this study also explored the use of cocoa waste combined with animal manure for energy generation through anaerobic digestion. Full article