Search Results (15)

Background: Shigella flexneri is a major cause of shigellosis in developing regions and is known to cause outbreaks in institutional settings. Transmission occurs via the fecal–oral route. It invades intestinal epithelial cells, causing diarrhea, systemic symptoms, and complications such as hemolytic uremic syndrome. This study aimed to characterize the clinical presentation, administered treatment, infection outcomes, and infection control measures during a local S. flexneri outbreak at a rehabilitation center. Methods: This case series at King Saud University Medical City (Oct–Dec 2024) investigated S. flexneri infections from a rehabilitation center. Stool and blood samples were cultured and analyzed using microbiological methods. Molecular studies were used to verify the genetic linkage between the isolates and to study their virulence genes. Results: Four cases of S. flexneri were included, involving patients with various comorbidities, residing in a rehabilitation center, and presenting with symptoms like fever and diarrhea. Laboratory investigations revealed leukocytosis, electrolyte imbalances, and elevated inflammatory markers. Imaging studies showed findings consistent with colitis in two cases. Patients were managed with IV fluids and targeted antibiotics, leading to symptom resolution. Molecular studies confirmed the genetic relatedness between the S. flexneri isolates, with virulence genes indicating cellular invasion and inflammation as primary drivers of disease severity. Outbreak management comprised contact isolation, environmental disinfection, and education. Conclusions: S. flexneri outbreaks in long-term care facilities pose challenges among bedbound patients. Diapers may facilitate transmission, and infections may cause severe complications. Robust infection control, identifying outbreak sources, and strengthening prevention strategies are essential to protect vulnerable populations. Full article

In response to Boschma’s concern that the implications of relatedness- and unrelatedness-based diversification strategies lack empirical evidence at disaggregated levels and in the context of the Global South, this study generates a unique dataset at the city level and explores how these smart specialization strategies (S3) may explain digital industry innovations within a specific regional innovation system, i.e., the Yangtze River Delta, China. The findings reveal that both relatedness density and knowledge complexity play a positive role in explaining digital industry innovations. However, the relationship between relatedness and knowledge complexity and its interactive effects on innovation performance are less straightforward. In our study, we found that efficient cooperation between relatedness and complexity can only be achieved if the level of government intervention is moderate. Therefore, the discussion of S3 focuses on more than the dichotomous argument between relatedness and unrelatedness. Many socio-economic factors also impact the effectiveness of these theoretical components within different innovation systems, which are largely overlooked by present studies. Full article

Urban parks are hot spots of bird diversity in cities. However, their role as urban filters for raptor species has not been assessed yet. This study aimed to compare the functional and phylogenetic traits of raptor assemblages in urban parks with the regional species pool of raptors in east–central Argentina. Diurnal raptors were surveyed in 51 urban parks in six cities during breeding and nonbreeding seasons. The regional species pool was assessed through raptor surveys and published maps surrounding the cities. The observed functional and phylogenetic relatedness of urban raptors was compared with 999 simulated raptor assemblages from the regional species pool. A total of five species were recorded in urban parks. The Chimango Caracara (Milvago chimango) was the numerically dominant species, comprising 95% of the 172 individuals recorded. The regional species pool was composed of 20 diurnal species. The functional and phylogenetic relatedness of urban raptors was higher than expected by chance, suggesting filtering induced by urban parks. Urban raptors were, in general, generalist species with small body sizes. Moreover, species tended to be part of the Falconidae family. Based on the results obtained here and in other published work, a model of raptor assembling in urban parks is proposed. The design of urban parks needs to be more heterogeneous, promoting the occurrence of specialist raptors. Full article

The COVID-19 pandemic has highlighted the importance of incorporating nature-based solutions in urban design, in order to create sustainable and resilient cities. Inspired by these events, the present study aims at exploring the mental health benefits of nature exposure during the outbreak. Secondarily, we investigate changes in use patterns towards urban green spaces (UGS) and urban blue spaces (UBS) and whether extreme conditions, such as these of a lockdown, can lead to an increase in people’s appreciation of urban nature. Through an online survey, we observed that the pandemic resulted in a decrease in the frequency of visitation to UGS/UBS (p < 0.001). Significant differences were found for exercise (p < 0.001) and socialization (p < 0.05) as main drivers for visiting urban nature pre- and post-lockdown. Accordingly, visitation rates for forests (p < 0.05), playgrounds (p < 0.001), and the sea (p < 0.001) differed significantly when comparing the two periods. In people’s perception, UGS/UBS are important for the urban fabric (89%). Our structural equation model indicated that nature exposure had a beneficial effect on participants’ mental health (p < 0.001). Pathways that explain the relationship between nature exposure and post- lockdown value were nature relatedness, motivation, and perceived importance of UGS/UBS. No mediation could be extracted for nature exposure and mental health. Our findings show the positive association between nature exposure and mental health improvement, especially in times of crisis, as well as a shift in the “value domain” towards urban nature. Full article

Coyotes are ubiquitous on the North American landscape as a result of their recent expansion across the continent. They have been documented in the heart of some of the most urbanized cities, such as Chicago, Los Angeles, and New York City. Here, we explored the genomic composition of 16 coyotes in the New York metropolitan area to investigate genomic demography and admixture for urban-dwelling canids in Queens County, New York. We identified moderate-to-high estimates of relatedness among coyotes living in Queens (r = 0.0–0.5) and adjacent neighborhoods, suggestive of a relatively small population. Although we found low background levels of domestic-dog ancestry across most coyotes in our sample (5%), we identified a male suspected to be a first-generation coyote–dog hybrid with 46% dog ancestry, as well as his two putative backcrossed offspring that carried approximately 25% dog ancestry. The male coyote–dog hybrid and one backcrossed offspring each carried two transposable element insertions that are associated with human-directed hypersociability in dogs and gray wolves. An additional, unrelated coyote with little dog ancestry also carried two of these insertions. These genetic patterns suggest that gene flow from domestic dogs may become an increasingly important consideration as coyotes continue to inhabit metropolitan regions. Full article

The present study aims to investigate whether a sense of relatedness to a city helps to broaden understanding of the restorative potential of urban public spaces. Findings based on a sample of German adults (n = 249) confirm that people experience relatedness to a city. The study’s 3 × 3 (built, mixed, natural environment) × (average, livability environment, bird’s-eye view) design revealed disordinal interactions for being away, fascination, preference, mental fatigue, and stimulating and activating effects associated with cities. This implies that humans’ place perceptions are more complex than previously assumed. Both city and nature relatedness were relevant covariates of these findings. Surprisingly, the construct ‘activating effects’, was found to be mostly perceived as more positive for mixed and built environments compared to natural environments. Thus, complementing restorative environments research by introducing a measure for city relatedness significantly enhances understanding of the potential of urban public spaces for promoting human health and well-being. Full article

This article examines the ways that Danish gang exit programs engage police officers and gang defectors in a pervasive work on belonging between gangs, kinship networks and the state. In urban Denmark, the majority of gang exit candidates are of ethnic-minority background and form part of the street-gang environment in marginalized migrant neighborhoods. This is an intimate social environment constituted by diasporic kinship networks, where gang formations are entangled with kinship formations. Hence, when gang defectors leave their gang, they also often leave their family and childhood home for a life in unfamiliar places and positions. As I show, gang desistance is thus a highly dilemmatic process in which gang defectors find themselves “unhinged” from meaningful social and kinship relationships and in search of new ways of embedding themselves into a social world. Based on an ethnographic study of gang exit processes in Denmark’s second largest city, Aarhus, this article shows how police officers and gang defectors seek to (re)shape ties of belonging between gangs, kinship networks and the state. The process, I argue, illuminates the intimate aspect of the notion of belonging, in which kin and state relatedness is deeply rooted in interpersonal spaces and relationships. Full article

Background: Cronobacter sakazakii is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the C. sakazakii isolates. Results: We isolated 25 C. sakazakii strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant C. sakazakii are required to control the contamination of this foodborne pathogen in infant foods. Full article

Socially disadvantaged youth are a group to which prejudices are attached which question the appropriateness of their participation, communication and language in the digital setting. From this perspective, the aim of the present research proposes to identify the forms and expressions of communication used by socially disadvantaged young people from the city of Madrid in social networks. This will be examined as a tool for the development of interpersonal and social relatedness processes, which will enable inclusion and socialisation in contemporary society. To this end, a qualitative approach was proposed which enabled a set of core memos, codes, networks and categories to be established, through which study data were interpreted. WhatsApp images and interviews conducted with 78 informants were analysed using Atlas.ti 9 software. All participants belonged to a social group characterised by circumstances of social vulnerability. Of the main findings, it should be indicated that these young people exhibit a social network use that is not limited to engagement in digital leisure but, instead, is based on the exchange of communication. For this reason, these individuals have developed their own language, a fact that highlights specific traits of the digital culture to which they belong and contributes to disproving the idea that these young people use the digital setting inappropriately. Full article

A growing number of policies and programmes in cities aim to increase the time people spend in nature for the health and wellbeing benefits delivered by such interactions. Yet, there is little research investigating the extent to which, and for whom, nature experiences deliver such benefits outside Europe, North America, and Australia. Here, we assessed the relationships between nature dose (frequency, duration, and intensity) and three mental wellbeing (depression, stress, and anxiety) and two physical health (high blood pressure, diabetes) outcomes in Singapore, an intensely urbanised tropical city. Our analyses accounted for individual factors, including socio-economic status, nature connection (nature relatedness), and whether people with poor health are prevented by their condition from visiting green spaces. Our results show that the association between nature dose (specifically duration) and mental wellbeing is moderated by a nature connection. Specifically, people with a stronger nature connection were less likely to be depressed, stressed, and anxious, regardless of the duration of their nature dose. For those with a weaker connection to nature, spending longer in nature was associated with being more depressed, stressed, and anxious. We did not find a relationship between nature dose and high blood pressure or diabetes. Our results highlight that the relationship between nature dose and wellbeing might vary substantially among cities. Full article

Chicken products and chickens with colibacillosis are often reported to be a suspected source of extraintestinal pathogenic Escherichia coli (ExPEC) causing several diseases in humans. Such pathogens in healthy chickens can also contaminate chicken carcasses at the slaughter and then are transmitted to humans via food supply; however, reports about the ExPEC in healthy chickens are still rare. In this study, we determined the prevalence and characteristics of ExPEC isolates in healthy chickens in China. A total of 926 E. coli isolates from seven layer farms (371 isolates), one white-feather broiler farm (78 isolates) and 17 live poultry markets (477 isolates from yellow-feather broilers) in 10 cities in China, were isolated and analyzed for antibiotic resistance phenotypes and genotypes. The molecular detection of ExPEC among these healthy chicken E. coli isolates was performed by PCRs, and the serogroups and antibiotic resistance characteristics of ExPEC were also analyzed. Pulsed-field gel electrophoresis (PFGE) and Multilocus sequence typing (MLST) were used to analyze the genetic relatedness of these ExPEC isolates. We found that the resistance rate for each of the 15 antimicrobials tested among E. coli from white-feather broilers was significantly higher than that from brown-egg layers and that from yellow-feather broilers in live poultry markets (p < 0.05). A total of 22 of the 926 E. coli isolates (2.4%) from healthy chickens were qualified as ExPEC, and the detection rate (7.7%, 6/78) of ExPEC among white-feather broilers was significantly higher than that (1.6%, 6/371) from brown-egg layers and that (2.1%, 10/477) from yellow-feather broilers (p < 0.05). PFGE and MLST analysis indicated that clonal dissemination of these ExPEC isolates was unlikely. Serogroup O78 was the most predominant type among the six serogroups identified in this study, and all the six serogroups had been frequently reported in human ExPEC isolates in many countries. All the 22 ExPEC isolates were multidrug-resistant (MDR) and the resistance rates to ampicillin (100%) and sulfamethoxazole-trimethoprim (100%) were the highest, followed by tetracycline (95.5%) and doxycycline (90.9%). bla_CTX-M was found in 15 of the 22 ExPEC isolates including 10 harboring additional fosfomycin resistance gene fosA3. Notably, plasmid-borne colistin resistance gene mcr-1 was identified in six ExPEC isolates in this study. Worryingly, two ExPEC isolates were found to carry both mcr-1 and bla_NDM, compromising both the efficacies of carbapenems and colistin. The presence of ExPEC isolates in healthy chickens, especially those carrying mcr-1 and/or bla_NDM, is alarming and will pose a threat to the health of consumers. To our knowledge, this is the first report of mcr-1-positive ExPEC isolates harboring bla_NDM from healthy chickens. Full article

While numerous studies examine the epidemiology and molecular characterization of Staphylococcus aureus in most developed countries, the detailed molecular characterization and molecular epidemiology of S. aureus strains and clones in Africa is lacking. We determined the molecular epidemiology and virulence of 81 non-duplicate isolates of S. aureus from Benin-City, Nigeria, collected during January–July 2016, and compared with global strains. Forty-seven isolates (58.0%) were found to be methicillin-sensitive Staphylococcus aureus (MSSA), while 34 (42.0%) were methicillin-resistant Staphylococcus aureus (MRSA). ST152-MSSA (24.7%) and ST7-MRSA-V (19.8%) were the dominant groups identified, which were not genetically related to global predominant strains, but rather exhibited regional dominance. An interesting finding of the study was the presence of highly related strains in the region, which differed primarily in their methicillin resistance gene carriage, staphylococcal cassette chromosome mec (SCCmec), with 99.4–99.7% relatedness between the genomes of the strains within the MRSA–MSSA pairs. This suggests that the strains within a pair are experiencing gain or loss of SCCmec within local conditions, with evolution continuing to diversify the strains to a small degree. This study represents the most comprehensive genetic and virulence study of S. aureus in Nigeria. Full article

To identify and evaluate architect Christopher Alexander’s theory of wholeness, this article draws on the work of British philosopher J.G. Bennett, who developed a conceptual method—what he called systematics—to clarify phenomena by drawing upon the qualitative significance of number. A central assumption of systematics is that there is something inherent in number itself that is fundamental to the way the world is and the way we can understand it. For Bennett, each whole number provides different but complementary modes for examining any phenomenon; thus, one-ness relates to the wholeness of the phenomenon; two-ness, to complementarity; three-ness, to relatedness, and so forth. This article draws on Bennett’s interpretation of four-ness, summarized by a diamond-shaped symbol that he called the tetrad. Bennett claimed that the tetrad provides an interpretive means for understanding any activity directed toward a focused outcome, for example, writing a book, designing a building, or planning a new city district. The tetrad is used in this article to probe and evaluate Alexander’s conceptual and practical efforts to recognize and fabricate wholeness, drawing on evidence from his Nature of Order and New Theory of Urban Design. The article first discusses the tetrad broadly and then considers how it helps to clarify Alexander’s efforts to understand and make wholeness. Full article

It is important to understand urban auto markets from a spatial perspective. Specifically, the question of how to simplify and visualize the relatedness of the complicated urban markets arises. Based on the concept of ‘product space’, this research explores the similarity between Chinese cities and identifies the city clusters using data of automobile sales in 2012. A city’s automobile market is shared by different manufacturers and the proximity between two cities is evaluated based on the similarity or relatedness in the structure of the two markets. The spatial structures of the ‘city clusters’ derived from the proximities of automobile markets among cities are mapped, examined, and interpreted. The analysis indicates that cities with higher proximity tend to be similar. According to the intercity proximity index, four geographical city-clusters are identified: the Southeast developed city-cluster, North China city-cluster, Northeast city-cluster, and West city-cluster. Cities in the same cluster tend to share many common characteristics while cities in different clusters exhibit obvious variances, especially in terms of economic status and dominant automakers. Full article

This study was undertaken to investigate the factors influencing proenvironmental behavior of individuals residing in the Northern Rocky Mountains (N = 267). Measures of relatedness to nature and perceived science knowledge were collected through a convenience sample approach using multiple avenues such as city email lists, organizational newsletters, and social media channels. Analysis of the data was conducted using both partial least squares and covariance based structural equation modeling to explore the relationships between the constructs. Additionally, qualitative definitions of proenvironmental behavior were investigated in order to address potential gaps between self-reported and observed behaviors. Quantitative findings show a renewed positive connection between science education, nature relatedness, and proenvironmental behaviors. Furthermore, qualitative findings suggest positive relationships between how publicly people are willing to share their passion for the outdoors and their willingness to engage in proenvironmental behaviors. Full article