Search Results (41,683)

The structural characterization of GG-rich DNA sequences in presence of metal ions provides essential insight into quadruplex stability and ion-dependent conformational specifics. We report the crystal structure of the GG-quadruplex formed by the sequence GGGGTTTTGGGG in the presence of Zn²⁺, K⁺, and Na⁺. It was deposited in the RCSB Protein Data Bank under the accession code 9FTA. The structure was determined by single-crystal X-ray diffraction at a resolution of 2.49 Å in the space group P2₁2₁2₁. It reveals a parallel-stranded, two-G-tetrad stabilized by K⁺ ions within the central channel, while Na⁺ and Zn²⁺ occupy peripheral and groove-associated sites. Zn²⁺ ions are engaged in noncanonical coordination interactions with phosphate oxygens and structured water molecules, contributing to lattice stabilization and subtle adjustments in groove dimensions. The T4 loop forms a compact, ordered motif that contributes to crystal packing rather than intramolecular G4 stabilization. The presence of mixed cations produces a sole lattice architecture mediated by ions that provides structural insight into how bivalent and monovalent metals mutually modulate G-quadruplex topology. These results suggest a basis for understanding the specific ion effects on G4 structures and may direct the design of metal open DNA architectures. Full article

This study examines the dynamic interactions between oil price shocks, monetary policy, and non-oil output in Saudi Arabia using Vector Autoregressive Model (VAR), and quarterly data spanning 2010: Q1–2025: Q3. The study aims to provide policy-relevant insights through which external oil price shocks and domestic monetary policy shocks affect inflation and non-oil economic activity in the context of Saudi Arabia’s structural transformation under Vision 2030. The results show that global oil prices behave largely as exogenous shocks, with limited feedback from domestic monetary conditions, implying that monetary policy effectiveness operates primarily through inflation and domestic demand channels rather than through oil prices directly. The findings underscore the importance of gradual and predictable monetary tightening, coordinated with fiscal and macroprudential policies, to mitigate the indirect spillovers of oil price volatility on the non-oil sector. While monetary policy plays a stabilizing role by containing inflation and supporting macroeconomic balance, sustaining diversification and non-oil growth under Vision 2030 requires complementary measures, including targeted credit support, financial market deepening, and structural reforms that enhance productivity and private-sector investment. Full article

Underwater acoustic sensor networks (UASNs) have emerged as a pivotal technology for ocean exploration, tactical surveillance, and environmental monitoring. However, the underwater acoustic channel poses severe challenges, including high propagation delay, limited bandwidth, and rapid time-varying multipath fading, which significantly degrade communication reliability. Cooperative communication, which exploits spatial diversity via relay nodes, offers a promising solution to these impairments. In this paper, we investigate the joint optimization of relay selection and power allocation in UASNs to maximize the long-term system energy efficiency and throughput. This problem is inherently complex due to the hybrid action space, which couples the discrete selection of relay nodes with the continuous allocation of transmission power, and the absence of real-time, perfect channel state information (CSI). To address these challenges, we propose a novel deep hybrid reinforcement learning (DHRL) framework utilizing a parameterized deep Q-Network (P-DQN) architecture. Unlike traditional approaches that discretize power levels or relax discrete constraints, our approach seamlessly integrates a deterministic policy network for continuous power control and a value-based network for discrete relay evaluation. Furthermore, we incorporate a prioritized experience replay (PER) mechanism to improve sample efficiency by focusing on rare but significant channel transition events. We provide a comprehensive theoretical analysis of the algorithm’s complexity and convergence properties. Extensive simulation results demonstrate that the proposed DHRL algorithm outperforms state-of-the-art combinatorial bandit algorithms and conventional deep reinforcement learning baselines in terms of system energy efficiency, and also exhibits superior robustness against channel estimation errors. Full article

Escalating climate volatility, particularly the El Niño/Southern Oscillation (ENSO), poses severe operational and financial risks to corporate sustainability in the energy sector. However, quantitative evidence regarding how macro-level climate shocks transmit to micro-level operational performance remains scarce. Integrating dynamic capability and social network theories, this study analyzes a panel of 103 Chinese listed energy firms (2005–2022) using System GMM, mediation, and moderation models. The results indicate that ENSO intensity significantly impairs performance; specifically, a 1 °C rise in sea surface temperature anomalies decreases firms’ return on assets (ROAs) by 0.142%. We identify supply chain resilience as a critical strategic mechanism for climate adaptation, where response capacity acts as the dominant mediating channel, while recovery capacity functions as an independent compensatory mechanism. Conversely, supply network complexity—across horizontal, vertical, and spatial dimensions—amplifies the negative impact of climate disruptions by hindering resource mobility. Heterogeneity analysis reveals that state-owned enterprises exhibit stronger institutional resilience, and firms in southern regions partially offset impacts through hydropower advantages. This study bridges climate science with operations management, offering strategic guidance for managers to configure resilient, sustainable supply chains capable of withstanding environmental turbulence. Full article

Forest fires occur frequently in China; however, the complex terrain and incomplete road networks severely constrain ground rescue efficiency. Accurate forest road information is essential for the optimization of emergency response and rescue force deployment. Existing road extraction algorithms are primarily designed for urban environments and exhibit limited efficacy in forest scenarios due to dense canopy, complex background interference and specific forest road features. To address this gap, this study proposes a forest road extraction method based on an enhanced DeepLabv3+ model using multi-temporal, high-resolution satellite imagery. Specifically, a Multi-Scale Channel Attention (MCSA) mechanism is embedded in skip connections to suppress background interference, while strip pooling is integrated into the Atrous Spatial Pyramid Pooling (ASPP) module to better capture slender road features. A composite Focal-Dice loss function is also constructed to mitigate sample imbalance. Finally, by applying the model in multi-temporal remote sensing images, a fusion strategy is introduced to integrate multi-seasonal road masks to enhance overall accuracy and topological integrity. Experimental results show that the proposed method achieves a precision of 54.1%, an F1-Score of 59.3%, and an IoU of 41.8%, effectively enhancing road continuity and providing robust technical support for fire-rescue decision-making. Full article

This study presents a modular and scalable wearable functional near-infrared spectroscopy (fNIRS) system for high-resolution cerebral hemodynamic signal acquisition. The system is based on compact optoelectronic modules and supports mixed measurements using short-separation and long-separation channels, offering good scalability and spatial adaptability. The integrated quartz light guide structure improves optical coupling efficiency between the probe and scalp. A series of in vivo experiments validated system performance. In a forearm arterial occlusion experiment, the system accurately captured concentration changes in oxygenated and deoxygenated hemoglobin during blood flow blockade and reperfusion, with large effect sizes (Cohen’s d > 0.9). In a prefrontal cortex Valsalva experiment, the biphasic response characteristic of neurovascular coupling was successfully resolved. In a 2-back working memory task, the system identified a task-related frequency component (0.0227 Hz) and right-lateralized prefrontal cortex activation (p = 0.023). These results demonstrate that the system exhibits a good signal-to-noise ratio and temporal dynamic response, enabling high-resolution mapping of regional hemodynamic changes. This work provides an effective solution for the development of wearable, modular, and high-precision multi-channel fNIRS systems. Full article

The 2025 approval of the selective NaV1.8 blocker suzetrigine for acute pain marked a pivotal advance in analgesic drug development. Yet the subsequent failure of Vertex’s next-generation NaV1.8 inhibitor VX993 to demonstrate clinical analgesia underscores enduring challenges in translating mechanistic promise into patient benefit. This review examines why promising targets and compounds, spanning NaV and TRP channels, often falter and outlines a path toward more reliable target selection and validation. I first summarize the pain pathway, from nociceptor transduction through spinal processing to cortical perception, emphasizing how inflammation and peripheral sensitization reshape excitability. Historically serendipitous, pain drug discovery now prioritizes molecular precision. Most approved chronic pain therapies act in the CNS and are limited by modest efficacy and adverse effects. Nociceptor-enriched targets (NaV1.7/1.8/1.9; TRP channels) remain attractive, yet redundancy among NaV subtypes and the necessity of blocking targets at the correct anatomical sites complicate translation. Human genetics and multi-omics provide a powerful, unbiased engine for target discovery. Rare high-impact variants offer strong causal hypotheses, while common polygenic contributions illuminate broader susceptibility. Large biobanks increasingly reveal a mismatch between legacy pain targets and genetically supported candidates across neuronal and non-neuronal cells. Human DRG transcriptomics highlight NaV channel redundancy. Human in vitro electrophysiology and PK/PD analyses show suzetrigine achieves ~90–95% NaV1.8 engagement, yet neurons can still fire unless additional channels are blocked. Species differences and drug distribution (including BBB/PNS penetration and P-gp efflux) critically influence efficacy; centrally accessible blockade (e.g., for NaV1.7 or TRPA1) may be necessary to achieve robust analgesia, challenging peripherally restricted strategies. Osteoarthritis illustrates how obesity-driven metabolic inflammation, synovial immune activation, subchondral bone remodeling, and specific nociceptor subtypes converge to drive mechanical pain. Multi-omic integration across diseased human tissues can pinpoint causal processes and cell types, enabling more selective and safer target choices. I propose a practical framework for target validation that integrates: (i) rigorous human genetic support; (ii) cell-type and site-of-action mapping; (iii) human-relevant electrophysiology and PK/PD with verified target engagement; (iv) species-appropriate models; (v) consideration of modality (small molecule, biologic, RNA, targeted protein degradation). Advancing genetically and anatomically aligned targets, tested at the right sites and exposures, offers the best path to genuinely effective, better-tolerated pain therapeutics. Full article

Unmanned Aerial Vehicle (UAV)-assisted hierarchical federated learning (HFL) has emerged as a promising architecture for Internet of Things (IoT)-based smart agriculture, which enables scalable model training over large and sparse farmlands. In this setting, UAVs act as mobile edge servers, aggregating local updates from distributed agricultural IoT devices and relaying them to the cloud server. While HFL improves scalability and reduces communication overhead, it still faces critical security threats due to its reliance on public wireless channels and the vulnerability of model aggregation to malicious updates. In this paper, we propose a secure authentication scheme that integrates anomaly detection with elliptic curve cryptography (ECC)-based mutual authentication to protect both the communication and training phases. In the proposed scheme, UAVs authenticate participating clients before receiving their local models, then perform anomaly detection to identify and exclude malicious participants. If a client is found to be malicious, its identity credentials are revoked and broadcast by the cloud server to prevent future participation. The security of the proposed scheme is formally verified using Burrows–Abadi–Needham (BAN) logic, the Real-or-Random (RoR) model, and the Automated Validation of Internet Security Protocols and Applications (AVISPA) tool, along with informal security analysis. The performance evaluation includes comparisons of security features, computation cost, and communication cost with other related schemes, and an experimental assessment of anomaly detection performance. The results demonstrate that our scheme provides strong security guarantees, low overhead, and effective malicious client detection, making it well suited for UAV-assisted HFL in smart agriculture. Full article

Serotonergic projections innervate both the dorsal and ventral cochlear nuclei; however, the electrophysiological consequences of serotonergic input in the ventral cochlear nucleus (VCN) remain incompletely understood. This study aimed to identify the serotonin receptor subtypes involved in serotonergic modulation of stellate cells in the mouse anteroventral cochlear nucleus (AVCN) and to determine the underlying ion channel mechanisms. Whole-cell patch-clamp recordings were performed in acute brain slices obtained from postnatal day 12–17 mice. Bath application of serotonin (25 µM) induced membrane depolarization (~5 mV) and increased action potential firing. Pharmacological experiments demonstrated that antagonists of 5-HT1A, 5-HT2A, and 5-HT2C receptors partially reversed the depolarization and reduced serotonin-induced inward currents, indicating that multiple receptor subtypes contribute to serotonergic excitation. Blockade of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels with extracellular Cs⁺ suppressed approximately 95% of the serotonin-induced depolarization and inward current, implicating HCN channel-mediated Ih as a principal ionic mechanism. Serotonin significantly increased Ih amplitude. Analysis of steady-state activation revealed no statistically significant shift in V0.5; however, under near-resting membrane potential conditions, serotonin significantly reduced the slope factor of the activation curve, consistent with altered voltage sensitivity of Ih gating. Immunohistochemical analysis confirmed the presence of 5-HT1A, 5-HT2A, and 5-HT2C receptors in the AVCN. Together, these findings indicate that serotonergic excitation of AVCN stellate cells is mediated by coordinated activation of multiple 5-HT receptor subtypes and primarily involves modulation of HCN-dependent subthreshold membrane dynamics. Full article

Remote sensing images acquired by UAVs under nighttime or low-illumination conditions suffer from insufficient illumination, leading to degraded image quality, detail loss, and noise, which restrict their application in public security and disaster emergency scenarios. Although existing machine learning-based enhancement methods can recover part of the missing information, they often cause color distortion and texture inconsistency. This study proposes an improved low-illumination image enhancement method based on a Weakly Paired Image Dataset (WPID), combining the Hierarchical Deep Convolutional Generative Adversarial Network (HDCGAN) with a low-rank image fusion strategy to enhance the quality of low-illumination UAV remote sensing images. First, YCbCr color channel separation is applied to preserve color information from visible images. Then, a Low-Rank Representation Fusion Network (LRRNet) is employed to perform structure-aware fusion between thermal infrared (TIR) and visible images, thereby enabling effective preservation of structural details and realistic color appearance. Furthermore, a weakly paired training mechanism is incorporated into HDCGAN to enhance detail restoration and structural fidelity. To achieve objective evaluation, a structural consistency assessment framework is constructed based on semantic segmentation results from the Segment Anything Model (SAM). Experimental results demonstrate that the proposed method outperforms state-of-the-art approaches in both visual quality and application-oriented evaluation metrics. Full article

We present a checkerboard metasurface integrating interleaved Helmholtz resonator arrays with distinct geometrical parameters, enabling decoupled dual-band coherent perfect absorption (CPA) in both in-phase and anti-phase excitation conditions. Full-wave simulations confirm that the proposed structure achieves absorption rates exceeding 99% at 2.904, 3.024, 3.788 and 3.856 THz, corresponding to two pairs of resonant modes enabled by the asymmetric transmission characteristics. Notably, by actively manipulating the relative phase difference between the two excitation modes, the absorption frequencies associated with each CPA channel can be independently and continuously tuned. Benefiting from the planar checkerboard configuration, which combines compact geometry, suppressed mutual coupling, and balanced energy distribution, the metasurface achieves stable and independent dual-band absorption characteristics. The proposed design provides a promising pathway for the development of terahertz coherent absorbers with enhanced frequency stability and spectral flexibility of dual-mode operations, offering strong potential for practical photonic and electromagnetic applications. Full article

In complex traffic environments such as low light, strong glare, occlusion and at night, systems that rely solely on visible light single sensors for pedestrian detection have drawbacks such as low detection accuracy and poor robustness. Based on the YOLOv8 convolutional network, this paper adopts a dual-branch structure to process visible light and infrared images simultaneously, fully utilizing feature information at different scales to effectively detect pedestrian targets in complex and changeable environments. To address the issues of insufficient interaction of modal feature information and fixed fusion weights, a cross-modal feature interaction and enhancement mechanism was introduced. A modal-channel interaction block (MCI-Block) was designed, in which residual connection structures and weight interaction were added within the module to achieve feature enhancement and filter out noise information. Introduce a dynamic weighted feature fusion strategy, adaptively adjusting the contribution ratio of different modal features in the fusion process, aiming to enhance the discrimination ability of the key pedestrian area. The training and testing of the network designed in this paper were completed on the visible light and infrared pedestrian detection dataset LLVIP and Kaist. At the same time, the test results of the dual-branch model and the model designed in this paper were further verified in actual traffic scenarios. The results show that the dual-branch YOLOv8 network for visible light and infrared images, which was constructed in this paper, can reliably enhance the detection performance of pedestrian targets in complex traffic environments, including accuracy, recall rate, and mAP@0.5, etc., thereby improving the robustness of pedestrian detection. Full article

Anti-unmanned aerial vehicle (Anti-UAV) detection is critical for airspace security, yet existing single-modality approaches suffer from severe performance degradation under adverse illumination, thermal crossover, and extreme scale variation. In this paper, we propose CSFADet, a dual-modal detection framework that jointly exploits visible and infrared imagery through four tightly integrated modules. First, a Cross-Spectral Feature Alignment (CSFA) module performs early-stage spectral calibration by computing cross-modal query–value attention maps, generating modality-aware channel descriptors that re-weight and concatenate the two spectral streams. Second, a Dual-path Texture Enhancement Module (DTEM) enriches fine-grained spatial details via cascaded convolutions with residual connections. Third, a Dual-path Cross-Attention Module (DCAM) introduces a feature-shrinking token generation strategy followed by symmetric cross-attention branches with learnable scaling factors, Squeeze-and-Excitation recalibration, and a $1\times 1$ convolution fusion head, enabling deep bidirectional interaction between modalities. Fourth, a Dual-path Information Refinement Module (DIRM) embeds Adaptive Residual Groups (ARGs) that cascade Multi-modal Spatial Attention Blocks (MSABs) with channel and dynamic spatial attention, culminating in a Multi-scale Scale-aware Fusion Refinement (MSFR) unit that employs three parallel multi-head attention branches with a Scale Reasoning Gate and Channel Fusion Layer to produce scale-discriminative enhanced features. Experiments on the public Anti-UAV300 benchmark show that CSFADet achieves 91.4% mAP@0.5 and 58.7% mAP@0.5:0.95, surpassing fifteen representative detectors spanning single-stage, two-stage, YOLO-family, and Transformer-based categories. Ablation studies confirm the complementary contributions of each module, and heatmap visualizations verify the model’s capacity to focus on small, distant UAV targets under challenging conditions. Full article

The push for higher energy density in electric vehicles has resulted in large-sized lithium-ion batteries, but their geometric upscaling exacts a heavy thermal price. Under high-rate discharge, these massive cells become heat traps, risking thermal runaway. To tame this instability, this paper engineered a hybrid management strategy fusing liquid cooling, Phase Change Materials (PCMs), and flow deflectors. With a primary focus on the structural optimization of the cooling channel, a three-dimensional numerical model, calibrated using experimentally determined thermophysical properties, was developed to overcome the thermal bottlenecks of conventional cooling architectures. Results indicated that the initial channel optimization effectively reduced the maximum temperature to 327.7 K, but it still remained near the safety threshold. Integrating PCM radically altered the thermal landscape, slashing the outlet temperature differential by 41.67% (from 2.76 K to 1.61 K) compared to pure liquid cooling and blunting peak thermal spikes. Furthermore, to overcome laminar stagnation, strategic deflector baffles were introduced to agitate the coolant, enhancing heat dissipation. Specifically, the optimal half-coverage (L = 1/2) baffle configuration successfully lowered the maximum temperature to 322.42 K while substantially reducing the system pressure drop from 948.16 Pa to 627.57 Pa, achieving a 33.33% reduction compared to the full-coverage scheme. Finally, a multi-variable sensitivity analysis confirmed the extraordinary engineering robustness of the optimized configuration, demonstrating a negligible maximum temperature fluctuation of less than 0.5% despite ±10% operational and material uncertainties. This synergistic system actively stabilizes the thermal envelope, offering a robust engineering blueprint for next-generation high-power battery packs. Full article