Search Results (2,599)

The Yellow River Delta (YRD), a crucial ecotone, is becoming increasingly polluted by antibiotics, posing serious threats to aquatic ecosystems and human health. In this study, comprehensive investigations were conducted to explore the regional distribution, environmental risks, and source apportionment of antibiotics, with the aim of facilitating precise management and control of antibiotic pollution. The results show that the surge in runoff during the water–sediment regulation period (June and August) of the Yellow River drove a sharp rise in antibiotic concentrations in the surface water, peaking at 135.0 ng/L, whereas antibiotics were rarely detected in the sediments after multiple rounds of intense hydraulic scouring (0.2~12.6 ng/g in October). Furthermore, seven antibiotics (sulfadiazine, sulfamethoxazole, flumequine, ofloxacin, tetracycline, doxycycline, and lincomycin) in surface water and six antibiotics (norfloxacin, enrofloxacin, ofloxacin, doxycycline, oxytetracycline, and florfenicol) in sediments were identified as representative compounds according to the antibiotic pollution profiles. Environmental risk assessment coupled with spatial autocorrelation analysis revealed that sulfamethoxazole generally posed medium to high risk (0.12~1.27) in surface water. Sediments posed more serious ecological risks, with universally high risk levels (ranging from 1.11 to 280.00). More importantly, in both surface water and sediment, four core antibiotic sources—namely, human sewage, livestock farming, agricultural and aquaculture inputs, and hydrodynamic-driven resuspension processes—were consistently identified through the Positive Matrix Factorization model and Kriging interpolation. These findings provide crucial insights for establishing targeted antibiotic pollution control strategies in the YRD and advance the understanding of antibiotic fate in sediment-laden rivers. Full article

An emerging environmental pollutant, microplastics have garnered global attention due to their widespread presence in soil and aquatic ecosystems. Early research primarily treated microplastics as single pollutants, focusing on their individual toxic effects. However, microplastics in the environment exist as a complex mixture, comprising various polymer types, sizes, shapes, and aging states. This diversity influences how microplastics regulate ecosystem carbon and nitrogen cycles and intervene through pathways such as direct carbon input, physical disturbance, microbial community restructuring, and coupled effects. This paper systematically reviews the characteristics of microplastic diversity and its mechanisms influencing carbon and nitrogen cycles: the chemical structure of polymers determines bioavailability and degradation rate, with biodegradable plastics altering carbon and nitrogen transformations more significantly than conventional plastics; microplastics of different sizes affect nitrogen transformation dynamics by modulating specific surface area and microbial colonization, with small-sized biodegradable microplastics particularly inhibiting plant nitrogen uptake; aging modifies surface properties and dissolved organic carbon release, thereby enhancing their role in promoting greenhouse gas emissions. Existing studies are largely confined to short-term laboratory simulations, leaving a gap in understanding the comprehensive effects of microplastic diversity under long-term, field conditions. Future research should focus on standardized methods and long-term experiments with multi-factor coupling to provide a scientific basis for ecological risk assessment of microplastic pollution. Full article

Sustainable water remediation requires catalytic strategies that remove contaminants efficiently while reducing chemical input, byproduct formation, and ecological disturbance. Conventional radical-dominated advanced oxidation processes can rapidly degrade pollutants, but their reliance on high oxidant dosages and freely diffusing reactive oxygen species often causes matrix quenching, non-selective oxidation, low oxidant utilization, and potential ecological risks. Mild interfacial catalysis provides a materials-chemistry strategy to regulate oxidative intensity and direct contaminant transformation under environmentally relevant conditions. In this review, mild catalysts are defined by pathway-selective, interfacially confined, and environmentally compatible oxidation rather than by low dosage alone. Representative non-radical or low-intensity pathways, including singlet oxygen generation, surface-mediated electron transfer, high-valent metal–oxo species, and direct oxidative transfer processes, are discussed in relation to active-site structure, oxidant utilization, matrix tolerance, and byproduct control. We further summarize how coordination environments, defect chemistry, heteroatom configurations, nanoconfinement, and immobilized interfaces regulate reactive-species formation and interfacial charge transfer. Key material platforms, including single-atom catalysts, heteroatom-doped carbons, defect-engineered oxides, catalytic membranes, hydrogels, and floating or immobilized composites, are evaluated from mechanistic and application-oriented perspectives. Finally, catalyst regeneration, cost, microbial community responses, algae–bacteria balance, ecotoxicity, and long-term safety are discussed to guide sustainable aquatic ecosystem restoration. Full article

Slaughterhouse wastewater introduces potentially toxic elements into aquatic ecosystems, yet bioaccumulation patterns in commercial fish species and associated human health risks remain underexplored in West Africa. This study quantified zinc (Zn), lead (Pb), iron (Fe), magnesium (Mg), chromium (Cr), and cadmium (Cd) in three ecologically distinct fish species—Oreochromis niloticus (Nile tilapia), Clarias gariepinus (African sharptooth catfish), and Mugil cephalus (Flathead grey mullet)—from two slaughterhouse-impacted rivers (Transamadi and Mgbuosimini) and a control site (Iwofe) in Rivers State, Nigeria. Metal concentrations were measured using atomic absorption spectrophotometry. Two-way ANOVA assessed species and location effects. Principal component analysis (PCA) was performed, with Mg used as a potential geogenic tracer, as its loading pattern was independent of Pb and Cd and consistent with the natural background. A Water Quality Index (WQI) classified Mgboshimini and Iwofe as having poor water quality (WQI > 75), while Transamadi had medium quality. Health risks were evaluated using estimated daily intake (EDI), target hazard quotients (THQ), and hazard indices (HI) following USEPA guidelines. Metal levels varied significantly by species and location (p < 0.001). Flathead grey mullet from Mgbuosimini had the highest Pb (1.50 ± 0.05 mg/kg) and Cd (0.41 ± 0.02 mg/kg), exceeding EU maximum levels for fish muscle (Pb 0.30 mg/kg, Cd 0.05 mg/kg) by 500% and 800%, respectively. PCA explained 77.5% of the variance, with Pb and Cd clustering as anthropogenic sources, while Mg loaded independently. THQ for Pb approached unity in Flathead grey mullet (0.88), and THQ for Cd reached 0.97. HI exceeded 1.0 in all species from Mgbuosimini, peaking at 2.07 in Flathead grey mullet. Uncertainty analysis (using ±SD) gave a HI range of 1.89–2.25 for this species, all above the safety threshold. Carcinogenic risk for Flathead grey mullet (3.97 × 10⁻⁴) approached the upper acceptable limit. Slaughterhouse effluent appears to elevate Pb and Cd burdens in fish, with detritivorous Flathead grey mullet posing the highest health risk. Exceedance of safety thresholds and HI > 1.0 indicate potential non-carcinogenic and carcinogenic risks. We recommend improved wastewater treatment and species-specific consumption advisories. Full article

Introduction: Biological invasions constitute one of the main threats to freshwater ecosystems, causing significant ecological changes through the introduction of exotic species that compete with or prey upon native species. In Portugal, the introduction and spread of exotic species in lotic and lentic ecosystems, such as pike (Esox lucius), European catfish (Silurus glanis), and largemouth bass (Micropterus salmoides), all top predators, may have a big impact on autochthonous species. In contrast, bleak (Alburnus alburnus), European perch (Perca fluviatilis), and common carp (Cyprinus carpio) compete aggressively for food resources. In the Sousa River basin, gudgeon (Gobio lozanoi) is considered an exotic species with potential ecological impact, with the minnow (Phoxinus phoxinus) stand having been recently identified in Portugal and, so far, recorded only in this river basin, and not yet being classified as an invasive species in Portugal. Public knowledge regarding invasive aquatic biodiversity remains a significant bottleneck for conservation. Because recreational angling is a prominent dispersal vector, initiatives that directly target this community are relevant. Objective: The aim is to carry out a bibliographic review on the exotic ichthyofauna species present in the Sousa River, with special focus on the invasion potential of the minnow (P. phoxinus). Methodology: The literature review was conducted based on the ScienceDirect, Springer Nature Link, and Fauna Norvegica databases, selecting publications between 2006 and 2025 concerning relevant studies on the potentially invasive characteristics of the minnow (P. phoxinus). The methodology is based on the analysis of studies regarding the impacts caused on riparian ecosystems. Results: The species P. phoxinus presents a generalist diet and high adaptive capacity, allowing it to colonise new habitats and compete aggressively with native species for trophic resources. Its presence is associated with negative impacts on brown trout populations (Salmo trutta), reducing growth and productivity, especially in mountain ecosystems. Increased species density also causes a significant decrease in benthic macroinvertebrate biodiversity. Studies conducted in the Douro basin indicate that the arrival of minnow in Portugal resulted from human action, probably associated with its use as live bait in recreational fishing. Conservation programmes use diverse tactics to bridge the awareness gap. Recent initiatives feature electrofishing demonstrations to visually differentiate species, theatrical performances, and even culinary show-cooking events using invasive predators like the European catfish to promote harvesting. Conclusions: The potential transition of P. phoxinus into an exotic and invasive species may be associated with the ecological pressure exerted on native communities, particularly through competition for trophic resources, highlighting the need to assess its dispersion in the Sousa basin and its impacts on fish fauna and benthic macroinvertebrates. It is important to do more sampling to understand its real distribution in the Sousa Basin. Additionally is important to explain to recreational anglers and the general population the impacts of fish transfer and the adverse effects of invasive species on freshwater Portuguese ecosystems. Full article

The presence of organic micropollutants such as pharmaceuticals and pesticides in aquatic systems poses risks to environmental and public health, as conventional wastewater treatment plants are often ineffective at removing them, highlighting the need for alternative solutions. This study evaluates the combined use of biochar and laccase to remove trimethoprim, clindamycin, and fipronil, selected for their ubiquity, persistence, and physicochemical properties. Commercial wood-derived biochar was used, and removal performance was assessed through adsorption isotherms, time-dependent evaluation of removal efficiency, and quantification by UPLC-MS/MS. Toxicity after treatment was evaluated using bacterial growth assays with Escherichia coli and Rhodococcus erythropolis. Adsorption of trimethoprim and clindamycin followed the Langmuir model (Qmax 2.27 and 1.49 mg/g), while that of fipronil followed the Temkin model (Qmax 0.98 mg/g). The combined biochar–laccase system enabled up to 99% removal of trimethoprim and clindamycin within one hour, demonstrating synergy between adsorption and enzymatic removal. Enhanced removal was also observed for clindamycin and fipronil in mixtures. Bacterial assays showed partial restoration of growth after treatment, suggesting reduced antibacterial activity of transformation products, although effects remained species-dependent. Overall, the biochar–laccase system shows promise for micropollutant removal, supporting green remediation strategies, but further work is required to characterize transformation products and assess ecological impacts. Full article

Microplastics are ubiquitous environmental particles with complex physical and chemical properties that enable them to interact with other contaminants. Recent evidence suggests that microplastics act as carriers for various chemical pollutants, altering their transport, deposition, and deposition dose. This conceptual review synthesizes current knowledge of radon progeny behavior and microplastic properties and suggests potential mechanisms for their interaction, although direct experimental validation of radon progeny specifically is currently lacking. It discusses attachment kinetics, transport pathways in air and water, and microplastic-mediated shifts in human lung deposition patterns and ecological exposure. Theoretical dosimetry reasoning suggests that, if attachment occurs, small respirable microplastics (1–10 μm) could increase inhalation doses by prolonging the airborne residence time of progeny indoors, whereas macro- and coarse microplastics would primarily affect localized environmental hotspots. These possibilities remain to be tested experimentally. Integrated experimental and modelling approaches, including radon chamber studies, aerosol and aquatic transport experiments, respiratory tract modelling, and ecological bioassays, are proposed to quantify these processes and inform risk assessment. Knowledge gaps remain in attachment efficiency, retention, co-contaminant interactions, and long-term exposure scenarios. Addressing these gaps is critical for refining human and ecological risk assessments and guiding regulatory frameworks in radon-microplastic-impacted environments. Full article

Introduction: The circum-Mediterranean region is a global biodiversity hotspot, hosting a highly distinctive freshwater fauna with a high degree of endemism and conservation concern. However, these ecosystems are increasingly threatened by biological invasions, particularly by non-native fish species, which represent a major driver of biodiversity loss. Objective: This study aims to compile a comprehensive and updated inventory of non-native inland fish species across the circum-Mediterranean region and to identify the main taxonomic, biogeographical, and socio-environmental drivers shaping their distribution. Methodology: We conducted an extensive review of the scientific literature, online databases (including EASIN, GISD, and CABI), and technical reports to compile records of non-native fish species across inland and transitional waters of Mediterranean-climate basins. Analyses focused on species composition, taxonomic representativeness, introduction pathways, native regions, and the relationship between species richness and selected environmental and socio-economic variables. Results: A total of 151 non-native fish species were recorded across the study area. Italy, Spain, Bosnia and Herzegovina, France, and Croatia exhibited the highest numbers of established species. Taxonomic representation was uneven, with Salmoniformes and Esociformes overrepresented among established non-native species, while Siluriformes and Characiformes were underrepresented. Most introductions originated from Europe, Asia, and North America, primarily through intentional releases and escape events. Non-native species richness was positively correlated with gross domestic product, precipitation, and the number of dams, highlighting the role of economic development and habitat modification in facilitating invasions. Conclusions: Biological invasions by non-native fishes are widespread across the Mediterranean basin and are strongly driven by human activities and environmental conditions. The high invasion levels observed in this biodiversity hotspot pose a significant threat to endemic freshwater faunas. These findings underscore the need for coordinated transnational management strategies, stricter regulation of introduction pathways, and prioritization of high-risk species to mitigate further impacts. Full article

Rapid urbanization and industrialization have profoundly affected aquatic ecosystems in urban rivers, with phytoplankton taxa and functional group composition being particularly sensitive to environmental changes. Field surveys were conducted in the Nanming River, Guiyang, in October 2018 and July 2019, with 33 sampling sites evenly distributed across the upstream, midstream, and downstream reaches. The results revealed that: (1) The phytoplankton community comprised 6 phyla, 53 genera, and 61 species, dominated by Bacillariophyta, Chlorophyta, and Cyanobacteria. The community was classified into 20 functional groups, among which B, D, MP, P, and S1 were dominant and exhibited clear spatial heterogeneity along the longitudinal gradient. (2) Analysis of variance indicated that physicochemical parameters were the dominant factors explaining the variation in phytoplankton taxonomic and functional groups, with their independent contribution significantly higher than that of anthropogenic disturbance indicators and geographical factors. Redundancy analysis further identified NH₄-N, TP, and TN as key environmental factors. Spearman’s correlation analysis further indicated that human activities alter ambient environmental conditions, which are significantly correlated with dissolved oxygen and chlorophyll a levels, thereby driving the differentiation of phytoplankton niches. (3) Functional group succession followed a distinct spatial pattern: upstream areas were dominated by groups P, SN, and Y, reflecting agricultural non-point source inputs; midstream areas were dominated by groups W1, H1, and S1, characteristic of urban complex pollution; and downstream areas were dominated by groups C and X1, indicating cumulative nutrient loading. Collectively, this study elucidates the driving mechanisms of phytoplankton dynamics in karst urban rivers and provides a scientific foundation for water quality monitoring, eutrophication risk pre-warning, and aquatic ecological restoration. Full article

Baseline information on terrestrial wildlife communities and their activity patterns is essential for protected-area management, but such information remains limited for Hunan Zhangjiajie Giant Salamander National Nature Reserve, where conservation attention has historically focused on the Chinese giant salamander and associated aquatic ecosystems. From March 2024 to August 2025, we conducted a camera-trap survey in broad-leaved and coniferous forest habitats of the reserve to document terrestrial mammals and ground-dwelling birds, evaluate taxonomic completeness, and describe diel and seasonal activity patterns. Across 43 camera-trap stations and 16,314 effective camera-trap days, we recorded 59 wildlife species, including 18 mammals and 41 ground-dwelling birds. The assemblage included nationally protected, threatened, and Chinese endemic species, indicating that the reserve’s forest habitats support important terrestrial biodiversity in addition to its aquatic conservation target. Taxonomic completeness curves suggested that the current survey captured most camera-detectable mammal and ground-dwelling bird taxa under the present sampling design, although the results should not be interpreted as a complete inventory of the reserve’s total vertebrate diversity. Annual diel activity analysis of 11 focal species showed clear temporal differentiation among ecological groups: small and medium-sized carnivores were mainly nocturnal, ground-dwelling birds, and red-hipped squirrel were primarily diurnal, and ungulates showed mixed or crepuscular-to-nocturnal tendencies. Seasonal analyses based on bioclimatic periods showed interspecific differences in activity-density distributions between the cool-dry and warm-wet seasons. However, peak-shift reliability analysis indicated that most focal species retained broadly similar main activity peaks across seasons; masked palm civet was the only species showing reliable seasonal displacement of its main activity peak. Pairwise temporal overlap analyses described temporal co-occurrence patterns among selected sympatric species but should not be interpreted as evidence of direct interaction or niche differentiation. Overall, this study provides baseline data on camera-detected terrestrial vertebrates in the reserve and supports long-term monitoring, forest habitat management, and disturbance control for terrestrial mammals and ground-dwelling birds. Full article

Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant widely detected in aquatic ecosystems, but its subcellular targets and the mechanisms by which it disrupts light resource utilization in photosynthetic protozoa remain poorly understood at concentrations spanning environmentally typical to supra-environmental levels. Here, Euglena gracilis G.A. Klebs was exposed to PFOS at concentrations spanning environmentally typical (0.5 µg/L), hotspot-relevant (5 µg/L), and supra-environmental (50 µg/L) levels. Subcellular distribution, phototaxis, photosynthetic light reactions, and energy metabolism were investigated using isolated chloroplast assays, transcriptomics, and proteomics. TEM-EDS mapping revealed pronounced fluorine signal enrichment, attributable to PFOS, in the eyespot and chloroplasts. Eyespot fluorine enrichment was associated with impaired phototactic motility and an altered light perception threshold. PFOS did not acutely inhibit the maximum photochemical efficiency of photosystem II (Fv/Fm); instead, a transient upregulation of photosynthesis-related genes was observed, which weakened with prolonged exposure, whereas the photosynthetic electron transport rate (ETR) was significantly reduced. PFOS significantly reduced ATP levels and ETR, while Fv/Fm remained unchanged and non-photochemical quenching (NPQ) was elevated. Isolated chloroplast assays revealed that PFOS inhibits Mg²⁺-dependent ATP hydrolytic activity in the chloroplast-enriched fraction and impairs thylakoid electron transport, consistent with impaired chloroplast ATP synthase function, though the specific molecular target and mechanism remain to be conclusively demonstrated. Transcriptomic and proteomic analyses revealed compensatory upregulation of photosynthesis pathways but suppression of ATP synthesis and redox homeostasis. Collectively, our results suggest that PFOS impairs chloroplast ATP synthase function, accompanied by reduced ETR and elevated NPQ. Together with the eyespot-associated phototaxis impairment, these effects suggest that PFOS may dually disrupt light acquisition (behavioral) and light conversion (physiological) in E. gracilis. This dual impairment may compromise the ecological fitness of Euglena in PFOS-contaminated environments, especially under prolonged exposure. It should be noted that the subcellular fluorine mapping is qualitative, the phototaxis assay reflects population-level responses, and the ATP synthase impairment interpretation is indirect; the proposed mechanistic model remains a hypothesis requiring further direct experimental validation. Full article

Heavy metal(loid) pollution in watersheds surrounding mining areas originates from multiple and complex sources, posing persistent threats to terrestrial–aquatic ecosystems and human dietary safety. This study systematically investigated the pollution characteristics, spatial distribution, ecological risks and human health hazards of seven typical heavy metal(loid)s (As, Pb, Cr, Cd, Cu, Zn, and Ni) in the integrated water–soil–vegetable continuum of a mining-affected watershed in Southwest China. Field sampling was carried out in three functional zones with different mining disturbance intensities, and inductively coupled plasma mass spectrometry (ICP-MS) was used to detect heavy metal(loid) concentrations in all samples. Multiple pollution evaluation indices and the USEPA human health risk assessment model were adopted for comprehensive quantitative analysis. The results showed that 44.0% of surface water samples exceeded national permissible limits, with high-pollution areas concentrated in intensive mining zones, presenting moderate overall aquatic heavy metal(loid) pollution. Although the average concentrations of seven heavy metal(loid)s in riparian soils complied with Chinese agricultural soil screening standards, localized significant enrichment was observed for As (1.98 times), Cd (4.62 times), Cu (1.81 times), and Zn (2.72 times) compared with regional background values, causing mild comprehensive soil pollution. Farmland soils exhibited prominent Cu and Zn accumulation, and leafy vegetables in the study area suffered severe Pb and Cd pollution, with potential dietary exposure risks. Health risk assessment indicated that children face higher non-carcinogenic and carcinogenic risks than adults via soil hand-to-mouth exposure; dietary intake of vegetables leads to moderate carcinogenic risks for children caused by As and Ni exposure. Overall, this study clarifies the migration and enrichment rules of heavy metal(loid)s in the water–soil–vegetable system of mining watersheds, confirms the prominent ecological and human health risks of Cd, As and Pb in the study area, and provides targeted basic data for regional heavy metal(loid) pollution prevention and food safety management. Full article

Introduction: Aquatic chemical pollution is among the most worrying threats to ecosystem health. There is an ever-increasing variety of pollutant substances detected across the source-to-sea continuum, causing loss of biodiversity and ecological disequilibrium. Achieving cleaner and healthier systems relies on carrying out sustained, cost-effective, diagnosis and aquatic effects monitoring, within the adaptive management cycle. The available methods are, however, cumbersome, which creates a clear need for innovative expeditious approaches for low-cost surveillance monitoring. In the last decade, Raman Spectroscopy (RS) has gained wide recognition for application to biological questions, for its ability to uncover the complexity of molecules and their interactions. Various fields, from pharmacology to disease diagnosis and prognosis, have suffered an innovation revolution through the application of RS. In this technique inelastic light scattering of a small part of photons of an incident electromagnetic monochromatic light beam (ranging from near-infrared to visible or ultraviolet) is caused by the molecular vibration of chemical bonds. This results in shifts in energy, which indicate discrete vibrational modes of polarisable molecules, providing qualitative and quantitative assessments of the chemical composition and molecular structure of the sample. The technique shows high sensitivity, no need for sample preparation and the possibility of use in non-invasive and label-free analysis. Objective: The aim of this work is to present and discuss evidence about the application of Raman Spectroscopy (RS) to environmental diagnosis and aquatic effect monitoring of pollution. Methodology: The technique was applied to different biological models, i.e., diatoms, zebrafish embryos and larvae and freshwater snails. Quality assessments with diatoms were tested in environmental monitoring, while assessments with other models were done upon exposure to metals and organic contaminants. Results and conclusions: The Raman spectra obtained from the samples analysed comprised bands detected within the 800 to 2000 cm⁻¹ wavenumber range. These were related to bond vibrations of carbohydrates, DNA phosphate groups, proteins or CH, NH and OH stretching in lipids and proteins. Data analysis using chemometric methods clearly distinguished pollutant exposure from control sites or treatments, pointing out the potential for surveyance monitoring. The next steps include the comparison with other sensitive methods (e.g., locomotion and avoidance behaviours, omics methods) to assess efficiency and bring further mechanistic understanding. Full article

The Dniester Delta is one of the Earth’s biodiversity hotspots and is home to many fish species of conservation and economic value. This unique complex of aquatic and semi-aquatic wetland habitats is also essential for the diversity of Dniester River and Black Sea ichthyofauna due to its role as a natural safe buffer and as a shelter, feeding, reproduction, and smooth transitional area for numerous fish species. Climate change is causing constant sea level rises in the Black Sea, which is anticipated to impact the vital ecosystems and related biodiversity in the Dniester Delta and other lower flooding areas, including the key ecological taxonomic group of fish. From this sea water rise risk assessment study of a total of 41 fish species, 6 were found to be under very high risk in the studied areas, 12 under high risk, 17 under moderate risk, and 6 under low risk. Positive ecological feedback in fish can stimulate environmental change and is expected to be responsible for changes within the Dniester Delta region complex of ecosystems in the context of sea level rise in the Black Sea, in addition to the diverse matrix of aquatic and semi-aquatic ecosystems in the near Dniester River and Black Sea. Full article

Excessive fluoride intake from drinking water remains a public health concern in geogenic high-fluoride regions, yet direct evidence linking environmental fluoride levels to internal exposure in remote high-altitude areas is limited. This study integrated environmental monitoring with human biomonitoring to assess fluoride exposure and health risks in the Taruo Lake region of the Tibetan Plateau. Surface water (n = 45 for Taruo Lake; n = 8 for its tributaries) and groundwater samples (n = 4) were collected and analyzed for fluoride concentrations, and blood ionic fluoride (BIF) levels were measured in 122 local residents (47 adults, 75 children). The results showed that fluoride concentrations in most surface water tributaries of Taruo Lake and groundwater sources were below China’s drinking water standard, whereas those in Taruo Lake exceeded this limit (routine monitoring mean 2.54 mg/L; multi-site mean 2.79 mg/L). BIF levels were significantly higher in adults (0.126 ± 0.041 mg/L) than in children (0.075 ± 0.032 mg/L) and showed a positive correlation with age (r = 0.533, p < 0.001). Notably, 23.4% of adults and 1.3% of children exceeded 0.15 mg/L, an empirical threshold typical for healthy populations in non-endemic areas. Based on the hazard quotient (HQ) model recommended by the US EPA, most drinking water sources posed acceptable non-carcinogenic risks (HQ < 1). In contrast, Taruo Lake water presented an elevated risk (HQ > 1) in 2024 primarily due to the regional geological background, and although not used for daily drinking, this finding offers an indicative reference for local water management and risk prevention. This preliminary monitoring and biomonitoring assessment provides baseline data for future studies and underscores the necessity of continuous surveillance and evaluation of total dietary fluoride intake to protect the health of this vulnerable high-altitude population. Full article