Search Results (19)

Drosophila carlosvilelai and D. paraguayensis are species that were collected in the Pasochoa Protected Forest between 1996 and 1997. D. carlosvilelai was described incompletely as a new species from Ecuador. Regarding D. paraguayensis, recent collections have raised doubts about the validity of its records in Ecuador. Specifically, a new species found in the province of Napo fully matches the specimens of D. paraguayensis recorded in the Pasochoa Protective Forest. However, this new species does not match the description of D. paraguayensis by Vilela and Bächli. The collected specimens, preserved in alcohol, were analyzed at the Laboratorio de Genética Evolutiva. D. carlosvilelai was re-described based on the newly collected specimens. The species previously misidentified as D. paraguayensis is described as D. paraloewi sp. nov. and compared with D. paraguayensis, D. loewi, and D. hemiloewi. This analysis revealed that the aedeagus of D. paraloewi shares the dorsal structure of triangular projection on the aedeagus with D. loewi and D. hemiloewi; this morphological similarity suggests a closer phylogenetic relationship between them, rather than D. paraguayensis. Full article

Insects exhibit diverse ecological characteristics, but species identification is challenging due to high morphological similarity. Traditional methods require genitalia dissection, which damages specimens and flattens three-dimensional structures, potentially losing key morphological details. In this study, we evaluate the utility of Micro-CT (Computed Tomography) as a non-destructive alternative for species identification by comparing genitalia structures obtained through Micro-CT with those obtained through traditional dissection. Micro-CT enabled three-dimensional reconstructions of male genitalia and aedeagus, providing detailed views from multiple angles without physical damage. The aedeagus was also virtually separated in a digital environment, further enhancing morphological analysis. Using this approach, we identified three new species, Casmara fulvacorona sp. nov. from Sumatra, C. falcatussica sp. nov. and C. fuscatulipa sp. nov. from Taiwan, based on genitalia characteristics. In addition, we provide a checklist of all Casmara Walker, 1863 species reported to date, including these newly described species, to confirm and clarify the distribution of this genus. Our results demonstrate that the additional use of Micro-CT in insect species identification can provide a scientific basis for reviewing and increasing confidence in species identification based on genital dissection. Full article

Our study aimed to characterize mating behavior in virgin and once-mated males and females of Cyclocephala barrerai. We reared the specimens under laboratory conditions and recorded the age at which the organisms began their reproductive activity and the time they remained active. We characterized behavioral acts during mating in two groups—virgin and once-mated organisms. Transitions between behaviors were analyzed using Markov chains and the generated ethograms. The duration of behaviors and the percentage of transitions were compared between the two groups. To characterize the mating behavior of C. barrerai, we adopted a framework commonly used in the literature, dividing mating behavior into three phases: premating, mating, and postmating. (A) Premating: Males search for females and mount and stimulate them, which the females resist through fighting behaviors. (B) Mating: The male mounts the female, which moves around and then stops; the male performs specific movements of the antennae and head and inserts his aedeagus into the female. (C) Postmating: Males exhibit guarding behavior over females. Although differences in pre- and postmating behaviors were observed between virgin and once-mated organisms, the duration of the acts did not show significant differences. These results provide key information on the reproductive behavior of C. barrerai, contributing to a better understanding of their biology and sexual interaction patterns. Full article

Four new dung beetle species of the genus Onthophagus Latreille from Côte d’Ivoire, Burkina Faso, Bénin, and Cameroon are described, and a fragment of their COI gene is sequenced. The coprophagous Onthophagus (Trichonthophagus) sylviae sp. nov. from Côte d’Ivoire and Burkina Faso is most similar to Onthophagus pallidus d’Orbigny from Cameroon, but it is more elongated, more silky than shiny, and has no dark spot on the third elytral interstria. Onthophagus odikpatra sp. nov. (18th group of d’Orbigny) from Côte d’Ivoire, Burkina Faso, and Cameroon feeds on carrion, including dead millipedes and fish, and dung. It is most similar to Onthophagus baloghi Balthasar from Central Africa but has simple punctures on the sides of the pronotum. Onthophagus chinonophilus sp. nov. from Côte d’Ivoire and Burkina Faso, which feeds on freshly dead millipedes and is attracted by their quinonous defensive secretions; it also belongs to the 18th group in d’Orbigny’s classification. Small and medium individuals resemble small Onthophagus latigibber d’Orbigny but differ in shape and sculpture of the pronotum. Small individuals are also similar to Onthophagus tschadensis Balthasar (19th group) but differ in the shape of the aedeagus. The generalist feeder Onthophagus (Tiaronthophagus) necneavius sp. nov. (24th group) from Côte d’Ivoire, Burkina Faso, and Bénin is most similar to Onthophagus naevius from Zambia, DR Congo, and Tanzania, but it is smaller, shiny all over; the sides of pronotum behind anterior angles are almost straight, not distinctly emarginate, and the frons is densely covered with fine punctures. COI barcode sequences were provided for all four new species, but the poor sampling of Onthophagus in GenBank prevents meaningful analysis of species relationships on the basis of COI barcodes at this point. Full article

The Miocene volcano Mt. Choke in northern Ethiopia is known for its very species-rich Trechus fauna. In addition, the enormous morphological diversity that is expressed within a single subgenus, Abyssinotus, which is endemic to northern Ethiopia, is unique within the global Trechus fauna. In this paper, we describe eight additional new species and three subspecies of the Trechus subgenus Abyssinotus, all of which are endemic to Mt. Choke. These species belong to two different lineages within Abyssinotus, each differing from other lineages of the subgenus by certain morphological character states described in this paper and alternatively named the T. lobeliae and the T. basilewskianus subgroups. Several species of these subgroups are characterised by an apomorphical inverse male genital, with the right side up in repose. The T. lobeliae subgroup comprises two species and is monomorphic with respect to this character. The T. basilewskianus subgroup comprises eight species, including six species with monomorphically inverse male genitalia and two species with male genitalia monomorphically in the ‘normal’ position. These are the first examples within Trechinae in which inverse male genitalia are a species- or even group-specific characteristic. A brief summary of the distribution of this trait within the Carabidae is provided. We also present a new identification key for the Trechus species known so far from Mt. Choke. Full article

The Canthon indigaceus species group represents a Typical Neotropical distributional pattern with wide penetration into the Mexican Transition Zone. This group consists of three species: C. (C.) indigaceus LeConte; C. (C.) chiapas Robinson; and C. (C.) chevrolati Harold. In this study, a new species for the group is described, C. (C.) cuixmala, collected along the tropical region of the Pacific coast and the Balsas River basin. The taxonomic status of members of the group were evaluated by comparing morphological characteristics and their biogeographic distributions. An updated dichotomous key is provided to separate members of this species group. Full article

Feather mites of finfoots (Heliornithidae), a small gruiform family, are poorly and partly erroneously recognized. Grallolichus heliornisi sp. n. (Astigmata: Pterolichidae) is here described from the sungrebe Heliornis fulica as the first representative of the genus commonly found on close relatives of finfoots, Rallidae and Sarothuridae. This species belongs to the species group having ornamented dorsal shields and is morphologically most close to G. proctogamus inhabiting Eurasian coot (Fulica atra). Males of the new species differ from G. proctogamus mainly by the shape of opisthosomal lobes (triangular vs. rounded) and the aedeagus form (parallel sided vs. tapering distally). Females differ mainly by the shape of supranal concavity (open anteriorly vs. closed) and location of setae h1 in relation to supranal concavity (lateral vs. anterior). A key to known species of the genus Grallolichus is provided. The morphological analysis and descriptive characterization of this species, like much of the approximately 2500 feather mite species described to date, were based on mummified mite material preserved in 19th-century old museum bird specimens. These often-forgotten collections are the only source for the analysis of the acarofauna of many rare, unavailable wild or even extinct bird taxa. Full article

A new species of the subfamily Pselaphinae (Coleoptera: Staphylinidae) has been discovered on Mount Etna (Sicily, Italy) and is described herein as Bryaxis aetnensis sp. nov. The new species is closely associated with the Bryaxis difficilis group, a highly homogeneous group of species living in the regions of Sicily and Sardinia. Diagnostic features and distribution of Sicilian species of this group are treated and illustrated herein. Bryaxis aetnensis sp. nov. exhibits similarities to B. marinae but can be distinguished by the darker color, longer antennal scape and terminal palpomere, and in the aedeagus morphology. The distribution of B. aetnensis sp. nov. spans a wide altitudinal range, demonstrating a remarkable climatic tolerance across the slopes and diverse habitats of Mount Etna. This broad tolerance reflects the species’ probable high ecological plasticity, which may also contribute to the observed morphological variability among individuals from different sampling sites. The significance of this new discovery on Mount Etna highlights the need to intensify sampling efforts in the region. Strengthening protection for these unexplored environments is crucial, and it also aids in unraveling biogeographic questions about the fauna inhabiting the area. As a relatively young volcanic environment, species colonization has occurred recently, making it an intriguing subject of investigation. Full article

In taxonomy, qualitative methods are commonly used to analyze morphological characters, which can lead to dramatic changes in higher taxa. Geometric morphometrics (GM) has proven to be useful for discriminating species in various taxonomy groups. However, the application of geometric morphometrics in supraspecies classification is relatively scarce. In this study, we tested the controversial two subgenera classification of Chaetocnema with geometric morphometrics; a total of 203 Chaetocnema species representing 50% of all known species from around the world were selected for the analysis. We analyzed the shape of the pronotum, elytron, head, aedeagus, and spermatheca. The results showed that the two traditional subgenera distinctly differed from each other; therefore, we propose using two subgenera arrangements to facilitate the understanding and taxonomy of the Chaetocnema species (especially in the Oriental, Palearctic, and Nearctic regions). Additionally, the morphological diversity of the abovementioned structures of Chaetocnema was analyzed, and it was found that the highest morphological diversity was in the spermatheca, which was greater than that in the aedeagus. Our research demonstrated that GM could be useful for detecting morphological delimitation of the supraspecies taxa. It also showed that GM methods are applicable to insects as small as 2 mm in body size. Full article

A morphology-based phylogeny of the Holarctic leafhopper genus Planaphrodes Hamilton is reconstructed for the first time based on 39 discrete male adult morphological characters. The results support the monophyly of Planaphrodes, with the included species forming two monophyletic lineages defined mainly by the number and location of aedeagus processes. The position of Planaphrodes in the Aphrodini was resolved as follows: (Stroggylocephalus + (Anoscopus + (Planaphrodes + Aphrodes))). The fauna of Planaphrodes from China, Japan and Korea are reviewed and six species are recognized, including two new species: P. bifasciatus (Linnaeus), P. sahlbergii (Signoret), P. nigricans (Matsumura), P. laevus (Rey), P. baoxingensis sp. nov. (China: Sichuan) and P. faciems sp. nov. (China: Hubei). Acocephalus alboguttatus Kato, 1933 syn. nov. and Aphrodes daiwenicus Kuoh, 1981 syn. nov. are considered junior synonyms of Planaphrodes sahlbergii (Signoret, 1879). Planaphrodes bella Choe, 1981 is a junior synonym of Planaphrodes nigricans (Matsumura, 1912). A checklist and key to species of Planaphrodes are provided. Full article

A new subgenus of the genus Chrysolina Motschulsky, 1860, endemic to Yunnan Province of China, Ch. (Volosatik subgen. nov.) is described. All species inhabit highland forests. Seven species, including four species new to science, are recognized: Chrysolina fascinatrix Lopatin, 1998, Ch. liqingzhaoae Daccordi et Ge, 2011, Ch. wangi Lopatin, 2005, Ch. igori sp. nov., Ch. marinae sp. nov., Ch. genriki sp. nov., and Ch. ilyakabaki sp. nov. The representatives of this new subgenus are characterised by a small, broadly oval, convex, shining body with metallic dorsum, with maxillary palpus narrow, similar in both sexes, with pronotal lateral impressions wide and shallow or obsolete, without numerous large punctures, with elytral puncture rows paired, mostly regular, intervals flat, with tarsomeres 1–3 with entire sole, rather narrow in both sexes, with aedeagus bearing sensilla of different types ventro-laterally near apex, flagellum narrow. Photographs of habitus and male aedeagus are presented. Full article

To elucidate the taxonomic problems in species delineation within the Oligonychus punicae complex (O. punicae, O. mangiferus, and O. vitis) (Acari: Prostigmata: Tetranychidae), we performed morphological and molecular investigations on mite samples, collected from different hosts/countries. Thirty-nine samples of punicae complex, collected from Egypt, Pakistan, and Saudi Arabia (SA), did not show any considerable morphological differences in females and males. All 39 samples of the punicae complex resembled the original description of O. punicae, while the claimed Mexican O. punicae was distinctively different based on male aedeagus. Molecularly, the low nucleotide diversity ranged from 0% to 2.1% (ITS2-rDNA) and 0% to 1% (COI-mtDNA), and was observed among various DNA sequences of the punicae complex from Egypt, India, Israel, Pakistan, and SA, confirming their identity as one species. The high genetic divergence ranged from 17.2% to 18.8% (ITS2) and 9.2% to 10.2% (COI), observed between the claimed Mexican O. punicae and all other sequences of the punicae complex, indicating that the Mexican sample do not belong to O. punicae. Basing our findings on both morphological and molecular data, we can conclude that O. mangiferus and O. vitis are synonymized with O. punicae. Additionally, this study reveals that the claimed Mexican O. punicae needs to be re-identified. Full article

The systematic position and actual distribution of Recaredus rex, for a long time one of the most enigmatic lace bug genus and species, is very obscure because only the type specimen and three other individuals from India are known to date. In the present paper, we report the first records of R. rex from the Palaearctic region (Iran) and tropical Africa (Ghana). Based on the occurrence localities and climatic variables, we predict potentially useful ecological niches for this species using Maxent software. The areas with the best environmental conditions for R. rex indicated in our studies suggest its possible Palaeotropical distribution. Moreover, we regard these results as a good starting point for further searches for specimens of this species. This might help verify the hypothesis of the broad Palaeotropical distribution of R. rex and its oligo- or polyphagy. In addition, the lace bug genus Recaredus, based on the diagnostic characteristics provided for the tribe Acalyptaini, and the structure of aedeagus, is transferred from the tribe Ypsotingini to the Acalyptaini. A key to all genera currently included in the latter tribe is also provided. Full article

In this study, data are presented for the designation of the new subgenus (Bajacanthon) for Canthon obliquus Horn, 1894 (Deltochilini). The new subgenus Bajacanthon is described based on a multiple evidence approach using morphological and molecular characters. This new subgenus has some unique characters, and is the remnant of an old South American migratory movement into the Mexican Transition Zone. This beetle species has survived in isolation in the south half of the peninsula of Baja California (Mexico). Results based on molecular clock inference showed that the split between the lineages leading to the subgenus Bajacanthon and Boreocanthon took place nearly 16 Mya, whereas the radiation within Bajacanthon was dated to 3.7 Mya. Full article

A comparative morphological study of the members of the subgenus Metallotimarcha revealed that Caucasian Timarcha hummelii is a separate species, which differs from European members of the subgenus (T. metallica, T. corinthia, T. gibba) by the internal structure of male aedeagus, namely, manubrium of the endophallus. Morphology of manubrium is described for all species of the subgenus. External characters and the shape of male aedeagus do not permit separating the members of the subgenus distinctly. Examination of both external characters and endophallus structure in the specimens from the Caucasus revealed that T. armeniaca and T. hummelii starcki are the synonyms of T. hummelii hummelii, but not separate species or subspecies. Neotypes of T. hummelii and T. armeniaca are designated. Syntypes of T. hummelii starcki are examined. The key to the species for the subgenus Metallotimarcha is compiled. Full article