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Keywords = UHPLC-DAD-ESI/MSn profile

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18 pages, 925 KB  
Article
Differentiation of Phenolic Composition Among Tunisian Thymus algeriensis Boiss. et Reut. (Lamiaceae) Populations: Correlation to Bioactive Activities
by Rym Jaouadi, Artur M. S. Silva, Mohamed Boussaid, Imen B. H. Yahia, Susana M. Cardoso and Yosr Zaouali
Antioxidants 2019, 8(11), 515; https://doi.org/10.3390/antiox8110515 - 28 Oct 2019
Cited by 26 | Viewed by 4061
Abstract
Twelve Tunisian Thymus algeriensis populations growing wild in different bioclimatic zones, extending from the subhumid to the upper-arid bioclimates, were compared regarding their phenolic composition and their ability to serve as antioxidant, anti-acetylcholinesterase, and antibacterial agents. A significant variation of phenol profile was [...] Read more.
Twelve Tunisian Thymus algeriensis populations growing wild in different bioclimatic zones, extending from the subhumid to the upper-arid bioclimates, were compared regarding their phenolic composition and their ability to serve as antioxidant, anti-acetylcholinesterase, and antibacterial agents. A significant variation of phenol profile was observed between the analyzed populations, as assessed by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MSn) technique. Rosmarinic acid was the main phenolic compound in most populations (383.8–1157.8 µg/mL extract), but still, those from the upper-arid bioclimatic zone were distinguished by the presence of carvacrol (1374.7 and 2221.6 µg/mL extract), which was absent in the remaining ones. T. algeriensis methanolic extracts were found to possess a substantial antioxidant and anti-acetylcholinesterase activities, with significant variation observed between populations, which were correlated to their phenolic contents. The antibacterial activity of the extracts tested against seven bacteria was revealed only by populations collected from upper-arid bioclimate and mainly associated with the presence of carvacrol. Extracts revealed a bacteriostatic effect against all bacteria (MIC = 1.4 mg/mL). Yet, the bactericidal activity (MBC = 1.4mg/mL) was restricted to the gram-negative bacteria Escherchia coli. Full article
(This article belongs to the Special Issue Phenolic Profiling and Antioxidant Capacity in Plants)
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11 pages, 1753 KB  
Article
Scabiosa stellata L. Phenolic Content Clarifies Its Antioxidant Activity
by Naima Rahmouni, Diana C. G. A. Pinto, Noureddine Beghidja, Samir Benayache and Artur M. S. Silva
Molecules 2018, 23(6), 1285; https://doi.org/10.3390/molecules23061285 - 27 May 2018
Cited by 22 | Viewed by 5446
Abstract
The phenolic profile of Scabiosa stellata L., a species used in Moroccan traditional medicine, is disclosed. To obtain that profile the species extract was analyzed by ultra-high-performance chromatography coupled to photodiode-array detection and electrospray ionization/ion trap mass spectrometry (UHPLC-DAD-ESI/MSn). Twenty-five phenolic [...] Read more.
The phenolic profile of Scabiosa stellata L., a species used in Moroccan traditional medicine, is disclosed. To obtain that profile the species extract was analyzed by ultra-high-performance chromatography coupled to photodiode-array detection and electrospray ionization/ion trap mass spectrometry (UHPLC-DAD-ESI/MSn). Twenty-five phenolic compounds were identified from which isoorientin and 4-O-caffeoylquinic acid can be highlighted because they are the major ones. The antioxidant activity was significantly controlled by the fraction type, with the n-butanol fraction showing the highest antioxidant activity (FRS50 = 64.46 µg/mL in the DPPH assay, FRS50 = 27.87 µg/mL in the ABTS assay and EC50 = 161.11 µg/mL in the reducing power assay). A phytochemical study of the n-butanol fraction was performed, and some important flavone glycosides were isolated. Among them the tamarixetin derivatives—the less common ones—can be emphasized. This phytochemical study and polyphenolic profile can be correlated with S. stellata extracts in vitro antioxidant activity. Moreover, it can be regarded as an evidence of its medicinal use and can incentivize its consumption. Full article
(This article belongs to the Special Issue The Antioxidant Capacities of Natural Products)
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