Search Results (78)

The design of high-performance drilling fluid systems is of vital importance for the safe and efficient exploitation of natural gas hydrates. Incorporating appropriate nanoparticles into drilling fluids can significantly enhance drilling fluid loss control, wellbore stability, and hydrate inhibition. However, the combined effects of nanoparticles and conventional additives on hydrate inhibition in drilling fluid systems remain poorly understood. In this study, the influence of nanoparticles on hydrate formation was first evaluated in a base mud, followed by an investigation of their combined effects with common drilling fluid additives. The results demonstrate that hydrophilic nano-CaCO₃ particles exhibit hydrate inhibitory effects, with the strongest inhibition observed at 3.0%. Composite system tests (incorporating nanoparticles with sepiolite, filtrate reducers, and flow modifiers) revealed diverse effects on hydrate formation. Specifically, the combination of nanoparticles and sepiolite promoted hydrate formation; the combination of nanoparticles and filtrate reducers showed divergent effects. Mixtures of nanoparticles with 0.2% low-viscosity anionic cellulose (LV-PAC), carboxymethyl starch (CMS), and low-viscosity carboxymethyl cellulose (LV-CMC) inhibited hydrate formation, while mixtures with 0.2% sulfonated phenolic resin (SMP-2) and hydrolyzed ammonium polyacrylonitrile (NH₄-HPAN) accelerated hydrate formation. Notably, the incorporation of nanoparticles with 0.3% guar gum, sesbania gum, high-viscosity carboxymethyl cellulose (HV-CMC), or high-viscosity polyanionic cellulose (HV-PAC) resulted in the complete inhibition of hydrate formation. By contrast, the synergistic inhibition effect of the nanoparticle/xanthan gum (XC) composite system was relatively weak, with the optimal compounding concentration determined to be 0.3%. These findings provide critical insights for the development of drilling fluid systems in natural gas hydrate reservoirs, facilitating the optimization of drilling performance and enhancing operational safety in hydrate-bearing formations. Full article

Sesbania cannabina (Retz.) Pers., as a legume, has strong waterlogging tolerance, but the lack of genomic information limits the exploration of key genes and molecular mechanisms. In this study, single-molecule real-time technology was used to sequence stems RNA of two Sesbania varieties at five time points under waterlogging stress through the PacBio Iso-Seq platform. The full-length transcriptome information contained 42 Gb raw data, 32,503 transcripts with an average length of 1912.28 nt, N50 length of 2059 nt and GC content of 42.69%. A total of 32,143 coding sequences (CDSs), 1745 transcription factors (TFs), 282 long non-coding RNAs (LncRNAs), 7497 simple sequence repeats (SSRs) and 202 alternative splicing (AS) events were identified through sequence alignment and software analysis. The analysis showed that 10,075 transcripts were enriched in 137 KEGG pathways, and 519 transcripts were included in plant hormone signal transduction, of which 103 and 123 transcripts were, respectively, involved in the ethylene and auxin pathways. The assembly and annotation of full-length transcriptome data of Sesbania provided reliable and accurate genomic information for the exploration of key genes and the study of molecular mechanisms in stem response to waterlogging stress. Full article

In light of the absence of discrete-element simulation characteristic parameters for the precision-seeding simulation process associated with Sesbania, this study investigated the physical properties of “CAS Sesbania No. 6” Sesbania seeds and measured the characteristic parameters of the Sesbania seeds in order to build a discrete-element model. The inter-seed contact parameters were calibrated to improve the seed model’s accuracy through stacking experiments and Box–Behnken response surface methodology. The stacking angle of Sesbania seeds was determined to be 34.5°; this was used as the evaluation index when simulating the stacking experiments. The results indicated that the coefficient of collision recovery, static friction, and rolling friction (inter-seed) were 0.168, 0.339, and 0.083, respectively. Substituting the calibrated parameters into the model, the simulation yielded a simulated stacking angle of 34.9°. The simulation results and bench experiment outcomes were compared to validate the applicability of the Sesbania seed discrete-element model in the context of seeder-simulation experiments. The results indicated that the percentage errors for the replay-seeding rate and missed-seeding rate between the simulation experiment and the bench experiment were 5.90% and 5.84%, respectively. All test results satisfied the technical requirements, confirming the calibration results’ reliability and providing a theoretical basis for the optimization of the design and simulation of Sesbania precision-seeding devices. Full article

Intercropping legumes is an effective and sustainable planting pattern that has the benefit of decreasing chemical fertilizer input and improving the soil environment. However, the effects of chemical fertilizer reduction and intercropping different legumes on soil nutrients, microorganisms, and tea quality remain elusive. Hereby, compared with 100% chemical fertilizer (CK), Sesbania cannabina (SC) and Crotalaria pallida Blanco (CP) were selected as the intercropped plant with 70% chemical fertilizer to investigate its effect on soil nutrients, microorganisms, and tea quality. The results showed that compared with monocropping, intercropping legumes had greater concentrations of the soil labile organic matter, nitrate nitrogen, ammonia nitrogen, inorganic nitrogen, and alkali-hydrolyzable nitrogen. Intercropping systems significantly enhanced the content of non-ester-type catechins (catechin and gallocatechin) and ester-type catechins (epicatechin gallate). In SC, the content of gallocatechin, catechin, and epicatechin gallate increased by 146.67%, 107.69%, and 21.05%, respectively, while in CP, the content of these three compounds increased by 166.67%, 84.62%, and 19.08%, respectively. Significant differences in microbial composition were also observed under different systems. Actinobacteria, Rhodoplanes, and Thaumarchaeota were obviously enhanced in SC, while Rhodanobacter, Pseudolabrys, and Pedosphaera were manifestly improved in CP compared to those in the monoculture. Moreover, intercropping legumes significantly increased the abundances of CNP cycling functional genes, such as gpmB, mch, accD6, pgi-pmi, mcr, glmS, ACOX1 and fadB (carbohydrate metabolism), nirD and narI (nitrification), pmoB-amoB and hao (dissimilatory N reduction), and phoN (organic phosphoester hydrolysis). The relationship between intercropping systems and tea qualities was mainly established by soil nutrition and the abundance of C and N cycling functional microorganisms. This study provides more information on the relationship between soil nutrients, functional genes of microorganisms, and tea quality under tea/legume intercropping systems in tea plantations and offers a basis for the higher-performance intercropping pattern. Full article

Rice-based cropping systems are the predominant sources of livelihood for farmers in South Asia, where soil quality decline is a major concern. An experiment was conducted at Bhubaneswar, Odisha, India, in 2018–2019 and 2019–2020. It comprised two rice cultivars, ‘Manaswini’ (M) and ‘Hasanta’ (H), and three nitrogen management practices—100% soil test-based nitrogen (STN) (N₁₀₀), 75% STN + in situ green manuring (Sesbania) (N₇₅+GM), and 50% STN + green manuring (N₅₀+GM)—in the main plot. Meanwhile, sub-plots consisted of three tillage methods for succeeding toria–sweet corn, namely zero tillage (ZT), conventional tillage (CT), and a furrow-irrigated raised bed (FIRB). The experiment was laid out in a split plot design with four replications to assess system nutrient uptake, productivity, and soil quality. Both rice cultivars were equal in terms of system nutrient uptake. The N₇₅+GM practice recorded the highest system NPK uptake (304.1, 70.34, and 343.5 kg ha⁻¹, respectively), enhancing the system N uptake by 10.7 and 7.4%, P uptake by 18.8 and 12.2%, and K uptake by 9.8 and 9.6% over N₁₀₀ and N₅₀+GM, respectively. ZT recorded the highest system NPK uptake (299.9, 70.6, and 339.7 kg ha⁻¹, respectively). The most promising treatments, M+(N₇₅+GM)-ZT and H+(N₇₅+GM)-ZT, recorded the maximum system economic yield (10.72 and 10.64 t ha⁻¹), residue yield (22.36 and 21.98 t ha⁻¹), biological yield (33.07 and 32.63 t ha⁻¹), and relative soil quality index (0.84 and 0.91). Correlation and principal component analyses exhibited positive and close relationships between all biological yield and soil quality parameters, except for micro water-stable aggregates, the fungal population, and the bulk density. The application of N₇₅+GM in M or H rice and ZT in succeeding toria–sweet corn can sustain productivity and soil quality in Eastern India. Full article

Intensive agriculture is the chief cause of soil degradation, particularly in regions with low soil organic carbon status, such as semi-arid southern India. In the quest to attain sustainable yield and improved soil quality, conservation agriculture (CA) is being advocated and adopted globally, including in India. In this experiment, CA was implemented to investigate the synergistic impacts of tillage and weed management on soil quality index and system yield and to identify a remunerative treatment combination that can sustain system yield and enhance soil quality. Contrasting tillage practices (main plots) included the T₁: conventional tillage with cotton–conventional tillage with maize–fallow, i.e., no Sesbania rostrata (Farmers’ practice), T₂: conventional tillage with cotton–zero tillage with maize–zero tillage with Sesbania rostrata and T₃: zero tillage with cotton + Sesbania rostrata residues–zero tillage with maize + cotton residues–zero tillage with Sesbania rostrata + maize stubbles. Weed management tactics (sub-plots) were W₁: chemical weed control, W₂: herbicide rotation, W₃: integrated weed management and W₄: single hand-weeded control in a split-plot design with cotton–maize–Sesbania cropping system over 3 years, in a split-plot design. Principal component analysis (PCA) was performed using the soil quality index (SQI)-CAL Version 1.0 software tool to extract minimum datasets from measured soil properties. A total of 40 soil variables were analyzed at 60 DAS and after the maize harvest, then subjected to principal component analysis (PCA) and subjected to PCA in soil quality index (SQI)-CAL software as to choose variables, minimum dataset and obtain soil quality index. The following soil properties, soil organic carbon (SOC), silt fraction, available soil zinc (Zn), iron (Fe), potassium (K), nitrogen (N), pH, electrical conductivity (EC), soil carbon to nitrogen (C:N) and cation exchange capacity (CEC), were selected as indicators based on correlations, calculated PCA and adept opinions on texture and lime concretions of experimental soil. The soil quality index improved by 23.34% in the T₃W₄ compared to T₁W₁. The system yield was 51.79% higher with the adoption of T₃W₃ compared to T₃W₄ combinations. Therefore, considering both system yield and soil quality index, T₃ and W₃ were remunerative and the best treatment combination among all others to sustain both soil and crop productivity in this region. Full article

This review evaluates the cytotoxic potential of the Sesbania genus, with a focus on Sesbania sesban, Sesbania grandiflora, and Sesbania cannabina. These species, known for their diverse phytochemical compositions, exhibit notable cytotoxic effects that suggest their utility in natural cancer treatments. Compounds such as quercetin, kaempferol, and sesbagrandiforian A and B have been highlighted for their strong antioxidant and antiproliferative effects, further emphasizing their therapeutic potential. The genus Sesbania exhibits a wide range of in vitro and in vivo bioactivities. Extensive research on S. grandiflora has uncovered mechanisms such as the activation of caspase cascades and the induction of apoptosis, attributed to its rich content of flavonoids and alkaloids. Notably, sesbanimides derived from S. grandiflora seeds have demonstrated potent cytotoxic effects by disrupting mitochondrial function. While S. sesban and S. cannabina have been less extensively studied, early findings highlight their potential through the inhibition of key cancer pathways and the identification of bioactive compounds such as galactomannan derivatives and 2-arylbenzofurans. Notably, the galactomannan derivatives from S. sesban exhibit significant immune-modulating properties. Additionally, nanoparticles synthesized from Sesbania species, including Cadmium oxide and PEGylated silver nanoparticles, have demonstrated promising cytotoxic activity by disrupting mitosis and enhancing immune responses. While further research is warranted, the Sesbania genus offers a promising basis for the development of innovative anticancer therapies. Full article

Soil salinization is a serious threat to the ecological environment and sustainable agricultural development in the arid regions of northwest China. Optimal soil salinization amelioration methods were eagerly explored under different soil salinity levels. Sesbania and hairy vetch are salt-tolerant plants, and green manure improved the saline environment. In this study, two leguminous halophytic crops, sesbania (Sesbania cannabina) and hairy vetch (Vicia villosa), were planted under different salinity levels, combined with three saline soil improvement measures, namely gravel mulching, manure application, and straw returning. No improvement measures and no salinity treatment was set as the control (CK). This study was conducted to analyze the effects of soil salinization improvement measures on the growth and ion uptake of sesbania and hairy vetch as biological measures under different soil salinity levels. Sesbania under manure application absorbed the highest soil Na⁺ (2.71 g kg⁻¹) and Cl⁻ (2.66 g kg⁻¹) amounts at a soil salinity of 3.2 g kg⁻¹, which was 14.7% and 10.95% higher than under gravel mulching and straw returning, respectively. Na⁺ and Cl⁻ absorption of hairy vetch under manure application reached the highest value of 1.39 g kg⁻¹ and 1.38 g kg⁻¹ at a soil salinity of 1.6 g kg⁻¹, which was 24.46% and 22.31% higher than under gravel mulching and straw returning, respectively. Plant height and stem diameter as well as root growth and development of sesbania and hairy vetch were limited at soil salinities greater than 1.6 g kg⁻¹ and 0.8 g kg⁻¹. Overall, sesbania and hairy vetch effectively absorbed both soil Na⁺ and Cl⁻ under manure application, thus regulating soil salinity and reducing soil salinization. However, soil salinity levels greater than 3.2 g kg⁻¹ and 1.6 g kg⁻¹ not only weakened the ionic absorption capacity but also inhibited the growth and development of sesbania and hairy vetch. This study provides evidence that soil salt ion absorption by sesbania and hairy vetch is promoted effectively, ameliorating soil salinity, under manure application as compared to under gravel mulching and straw returning. Full article

Diabetic wounds present significant burdens to both patients and the healthcare system due to their prolonged inflammatory phase and adverse microenvironment. Traditional Chinese medicine (TCM), particularly Scutellaria baicalensis extract (SE), has shown promise in wound healing. Herein, sesbania gum (SG) was oxidized and formed hydrogel with carboxymethyl chitosan (CMCS) through the imine bond. Then, SE was loaded into the hydrogel as a wound dressing (CMCS−OSG@SE hydrogel). In vitro experiments demonstrated the mechanical properties and ROS scavenging efficiency of the hydrogel, as well as the release of SE and its biocompatibility. In an vivo study, diabetic mice with S. aureus infection were used, and the CMCS−-OSG@SE hydrogel dressing accelerated wound healing by promoting epidermal regeneration and collagen deposition. This composite polysaccharide hydrogel loaded with SE shows great potential for diabetic wound treatment. Full article

Planting salt-tolerant plants is an efficient method of biological improvement for saline–alkali land. However, few studies have examined the soil improvement effects of the rotation of the green manure plant sesbania and the grain crop sorghum. Thus, we planted sesbania in native soil on heavily saline–alkaline coastal land and subsequently planted sorghum after returning the sesbania straw to the soil. The effect of this sesbania–sorghum rotation on soil improvement was clarified by comparing indicators of soil quality before and after sesbania and sorghum were planted, such as the soil structure, water infiltration, soil salt content, and soil microbial changes. The results showed that the soil bulk density of the plow layer (0–40 cm) after crop harvest decreased by 9.63% compared with that of bare land, and the soil porosity increased by 5.67%. The cumulative infiltration, initial infiltration rate, and stable infiltration rate of saline soil were 3.6 times, 2.8 times, and 3.3 times higher than those of bare land, respectively. With the growth of sesbania and sorghum, the soil salt content in the plow layer of the cultivated land decreased by 37.73%, while that of bare land decreased by 9.1%. A further analysis of desalination showed that the total desalination amount in the plow layer was 15.58 t/ha, of which 5% was due to plant absorption, and the rest was from salt leaching. Moreover, sesbania–sorghum rotation increased the soil organic matter content in the plow layer from 69.1 t/ha to 73.8 t/ha. The quantities of some microorganisms that are mainly found in coastal saline soil decreased, while those of some common soil microorganisms increased, reflecting an improvement in the soil quality. The above results prove that sesbania–sorghum rotation had a significant effect on soil improvement and salt reduction, which is of great significance for the further utilization of saline–alkali land to enhance crop productivity. Full article

Intercropping with green manure is a soil-sustainable cultivation practice that has demonstrated positive impacts on tea growth and the soil environment in tea plantations. Nevertheless, research examining the effect of leguminous green manure varieties in tea plantations is scarce. This study aimed to analyze the tea quality and soil environment components in response to intercropping with three distinct leguminous green manures, Cassia sophera cv. Chafei 1 (CF), Sesbania cannabina (Retz.) Pers. (SC), and Chamaecrista rotundifolia (Pers.) Greene (CR), with 70% chemical fertilizer, and compare them to non-intercropped green manures with 100% chemical fertilizer (CK) in tea plantations. The findings indicated that intercropping with SC increased the amino acids content of tea leaves, the soil organic carbon (SOC), the soil acid phosphatase (ACP), the soil acid protease (ACPT), and the bacterial diversity compared to the CK treatment. Intercropping with CR improved the ACP activity and bacterial diversity while intercropping with CF improved the polyphenols. Proteobacteria, Acidobacteria, Actinomycetes, and Firmicutes were identified as the dominant bacterial taxa in tea plantations with intercropped green manure. A strong positive correlation was indicated between the SOC contents and the amino acids content in tea leaves after intercropping. A canonical correspondence analysis indicated significant associations between the ACP and the urease activity, and between the ACP and ACPT, and both were closely linked to SC. This finding provides an explanation that intercropping with SC may positively affect tea quality by influencing the SOC content, the soil enzyme activity, and the soil bacterial diversity. Green manure intercropping may replace part of chemical fertilizers, improve the soil environment in tea gardens, and enhance the quality of tea. These findings offer a theoretical reference for selecting leguminous green manure and advancing the sustainable development of tea plantations. Full article

In a continued search for legumes to grow with forage sorghum (FS) [Sorghum bicolor (L.) Moench] in semi-arid environments, studies in 2019 and 2022 at New Mexico State University Rex E. Kirksey Agricultural Science Center at Tucumcari, NM USA, evaluated FS mixed with cowpea [Vigna unguiculata (L.) Walp.], lablab (Lablab purpureus L.), both previously evaluated, and a native legume, big-pod sesbania (Sesbania macrocarpa), for yield and nutritive value in four randomized complete blocks each year. At harvest legume proportions of approximately 15% of the dry matter yield, there were no differences in the yield (mean = 15.97 Mg ha⁻¹, p > 0.40) or land equivalency ratio between sole FS and any mixture or among mixtures; however, the crude protein of FS+Lablab was greater than sole FS, with FS+Cowpea and FS+Sesbania intermediate (67, 77, 87, and 79 g kg⁻¹ for sole FS, FS+Cowpea, FS+Lablab, and FS+Sesbania, respectively; 5% LSD = 14). The neutral detergent fiber digestibility of FS+Sesbania was less than all other treatments (p = 0.0266). Although the sesbania did not improve forage yield or nutritive value when grown with FS and harvested near sesbania maturity, perhaps growing with a shorter season grass and harvesting earlier may show benefit, as sesbania’s nutritive value is known to be greater at earlier stages. Full article

Salt-affected soils are a prevalent issue globally, resulting in a severe degradation of soil sustainability and plant productivity, reducing the area of agricultural land, and affecting food security. Therefore, eco-solutions and remediation approaches are needed. The needed remediation for salt-affected soil can be addressed via engineering, physical, chemical, or biological techniques. Salt-tolerant crops are normally used for the remediation of slight and moderate saline soil conditions. However, no crops, including salt-tolerant crops, can be cultivated in areas with extreme salinity levels (ECe 8–16 dS/m). Therefore, the aim of this study was to investigate the effect of vermicompost and organic amendment on the cultivation of salt-tolerant crops (Sesbania rostrata) in severely salt-affected soil under field conditions in order to improve saline soil and crop productivity. The design of the experiment followed a randomized complete block design (RCBD) with three treatments and four replications: T1, severely salt-affected soil (control); T2, severely salt-affected soil + vermicompost at a ratio of 25:75; T3, severely salt-affected soil + vermicompost + rice husk biochar + coconut coir at a ratio of 25:25:25:25. The results found that using vermicompost and organic amendment improved the soil quality, increased the soil fertility (organic matter and plant nutrients N, P, and K), and reduced the soil salinity. Sesbania rostrate could not grow in severely salt-affected soil (T1) alone, but could grow in the treatments with vermicompost and organic amendments (T2 and T3). The percentage of Sesbania survival per plot was also high in the treatments with vermicompost and organic amendments (T2 and T3). The highest growth rate, flower production, biomass, and root morphology of Sesbania rostrata were found in T3, with severely salt-affected soil + vermicompost + rice husk biochar + coconut coir at a ratio of 25:25:25:25 with a statistically significant difference (p < 0.05). Moreover, the Sesbania flowers treated with vermicompost and organic amendments have a higher nutritional value due to their minerals and vitamins than Sesbania flowers grown without using vermicompost and organic amendments. This study’s findings suggest that incorporating vermicompost and organic amendments is a feasible and economical method for enhancing the quality of salt-affected soils in a sustainable manner. The results of this study demonstrate that utilizing vermicompost and organic amendments is a sustainable and economical strategy for enhancing the quality of salt-affected soils and improving yields in severely salt-affected areas, thereby increasing crop production and the nutritional value of the plants as well as helping to increase farmers’ income. Full article

Four pigment-producing rhizobial strains nodulating Sesbania cannabina (Retz.) Poir. formed a unique group in genus Microvirga in the phylogeny of a 16S rRNA gene and five housekeeping genes (gyrB, recA, dnaK, glnA, and atpD) in a genome analysis, phenotypic characteristics analysis, and chemotaxonomic analysis. These four strains shared as high as 99.3% similarity with Microvirga tunisiensis LmiM8^T in the 16S rRNA gene sequence and, in an MLSA, were subdivided into two clusters, ANI (genome average nucleotide) and dDDH (digital DNA–DNA hybridization) which shared sequence similarities lower than the species thresholds with each other and with the reference strains for related Microvirga species. The polar lipids elucidated that phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin were the main components for strain SWF67558^T and for strain HBU65207^T, with the exception of PC. SWF67558^T and HBU65207^T strains had similar predominant cellular fatty acids, including C16:0, C18:0, summed feature 2, and summed feature8, but with different contents. In addition, all the four novel strains produced pink-pigment, and the main coloring material extract from strain SWF67558^T was identified as zeaxanthin, which presented antioxidant ability and reduction power. With all the phylogenetic and phenotypic divergency, we proposed these pink-pigmented symbiotic bacteria as two novel species, named Microvirga sesbaniae sp. nov. and Microvirga yunnanensis sp. nov., with SWF67558^T (=KCTC82331^T=GDMCC1.2024^T) and HBU65207^T (=KCTC92125^T=GDMCC1.2023^T) as the type strains, respectively. Full article

Sustainable ecological agriculture is achieved by regulating the benefits of trees. The application of leguminous trees as alley cropping protects and increases soil fertility, improves the quality of water by intercepting pesticides, changes the local climate, improves biodiversity, and thus improves productivity. In order to evaluate the impact of alley cropping upon the growth and productivity attributes of fennel, an experiment was carried out during two seasons. The experiment included eight treatments. Fennel seeds were cultivated between Sesbania alleys and treated with N and endophytic fungi according to the eight treatments. After harvesting the fennel, different parameters were determined and biochemical analyses were conducted. All of the alley cropping treatments showed remarkable superiority in all measures of fennel growth and productivity compared to the sole crop treatment. Among the different alley cropping treatments, applying Sesbania at 4 m spacing with N fertilizer and EF increased most of the studied parameters in terms of the least number of days from planting until harvesting of fennel, herb dry weight, number of umbels, fruit yield, essential oil, N, P, and K content, and pigments. In contrast, the highest plant height was recorded with fennel–Sesbania at 2 m spacing + N fertilizer + EF. Applying fennel–Sesbania at 6 m spacing + N fertilizer + EF treatment resulted in higher stem diameter and increased the number of main branches compared to the other treatments. Full article