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12 pages, 8317 KiB  
Article
Loquat (Eriobotrya japonica) Is a New Natural Host of Tomato Mosaic Virus and Citrus Exocortis Viroid
by Chengyong He, Lingli Wang, Yarui Li, Kangyu Zhou, Ke Zhao, Dong Chen, Jing Li, Haiyan Song and Meiyan Tu
Plants 2024, 13(14), 1965; https://doi.org/10.3390/plants13141965 - 18 Jul 2024
Viewed by 1773
Abstract
Loquat leaves exhibiting obvious yellowing, blistering, mosaic, leaf upward cupping, crinkle, and leaf narrowing were identified in Panzhihua City, Sichuan Province, China. High-throughput sequencing (HTS) with the ribo-depleted cDNA library was employed to identify the virome in the loquat samples; only tomato mosaic [...] Read more.
Loquat leaves exhibiting obvious yellowing, blistering, mosaic, leaf upward cupping, crinkle, and leaf narrowing were identified in Panzhihua City, Sichuan Province, China. High-throughput sequencing (HTS) with the ribo-depleted cDNA library was employed to identify the virome in the loquat samples; only tomato mosaic virus (ToMV) and citrus exocortis viroid (CEVd) were identified in the transcriptome data. The complete genome sequence of ToMV and CEVd were obtained from the loquat leaves. The full-length genome of the ToMV-loquat is 6376 nt and comprises four open reading frames (ORFs) encoding 183 kDa protein, RNA-dependent RNA polymerase (RdRp), movement protein (MP), and coat protein (CP), respectively. A pairwise identity analysis showed that the complete sequence of the ToMV-loquat had a nucleotide identity between 98.5 and 99.3% with other ToMV isolates. A phylogenetic analysis indicated that ToMV-loquat was more closely related to ToMV-IFA9 (GenBank No. ON156781). A CEVd sequence with 361 nt in length was amplified based on the HTS contigs, sequence alignment indicated CEVd-loquat had the highest identity with the strain of CEVd-Balad (GenBank No. PP869624), phylogenetic analysis showed that CEVd-loquat was more closely related to CEVd-lettuce (GenBank No. ON993891). This significant discovery marks the first documentation and characterization of ToMV and CEVd infecting loquat plants, shedding light on potential threats to loquat cultivation and providing insights for disease management strategies. Full article
(This article belongs to the Section Plant Protection and Biotic Interactions)
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25 pages, 3034 KiB  
Article
Environmental Life Cycle Assessment of Two Types of Flexible Plastic Packaging under a Sustainable Circular Economy Approach
by Börçe Tunçok-Çeşme, Eren Yıldız-Geyhan and Gökçen Alev Çiftçioğlu
Sustainability 2024, 16(8), 3149; https://doi.org/10.3390/su16083149 - 10 Apr 2024
Cited by 3 | Viewed by 3503
Abstract
While it is of great importance to evaluate plastic waste within the framework of a circular economy today, it is also of great importance to evaluate flexible plastic packaging, which is increasingly used in order to prevent environmental problems. To avoid the disadvantages [...] Read more.
While it is of great importance to evaluate plastic waste within the framework of a circular economy today, it is also of great importance to evaluate flexible plastic packaging, which is increasingly used in order to prevent environmental problems. To avoid the disadvantages experienced in recycling due to its multilayer nature, in this study, a life cycle assessment was made for flexible packages consisting of PET/metallized PET/PE and PP/metallized PP/PP with the data provided by the R&D Center of Elif Plastik Ambalaj Sanayi ve Tic. A.Ş.-Huhtamaki Flexibles Istanbul. Within the scope of this evaluation, two types of flexible packaging were analyzed, and an optimal flexible packaging structure for Türkiye was revealed by comparing different scenarios based on different methods in SimaPro 8.1.1.16. LCA was performed for both packages with cumulative energy demand (CED) and CML-IA methods. Four scenarios with different amounts of recycled raw materials were compared against the existing system and a fifth scenario, where electricity is assumed to be obtained from solar energy. Overall, we found that the largest environmental impact was in the existing system. However, despite being a renewable energy source, we observed that the solar energy scenario had almost as significant an impact as the existing system. When scenarios involving recycled raw materials were examined, we clearly observed that as the amount of recycled raw materials increased, the environmental impact decreased. Therefore, it emerged that the scenario with the highest amount of recycled raw materials is the most optimal scenario in many respects. There are clear differences in the results due to differences in plastic types. This study, conducted with real data, is highly important for the flexible packaging literature. A table has been provided for changing the type of plastic, changing the source of electricity generation, and reducing waste by using recycled raw materials in order to make flexible packaging more environmentally beneficial. Full article
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22 pages, 6364 KiB  
Article
Genome-Wide Identification of the GhANN Gene Family and Functional Validation of GhANN11 and GhANN4 under Abiotic Stress
by Jin Luo, Meili Li, Jisheng Ju, Han Hai, Wei Wei, Pingjie Ling, Dandan Li, Junji Su, Xianliang Zhang and Caixiang Wang
Int. J. Mol. Sci. 2024, 25(3), 1877; https://doi.org/10.3390/ijms25031877 - 4 Feb 2024
Cited by 7 | Viewed by 2506
Abstract
Annexins (ANNs) are a structurally conserved protein family present in almost all plants. In the present study, 27 GhANNs were identified in cotton and were unevenly distributed across 14 chromosomes. Transcriptome data and RT-qPCR results revealed that multiple GhANNs respond to at least [...] Read more.
Annexins (ANNs) are a structurally conserved protein family present in almost all plants. In the present study, 27 GhANNs were identified in cotton and were unevenly distributed across 14 chromosomes. Transcriptome data and RT-qPCR results revealed that multiple GhANNs respond to at least two abiotic stresses. Similarly, the expression levels of GhANN4 and GhANN11 were significantly upregulated under heat, cold, and drought stress. Using virus-induced gene silencing (VIGS), functional characterization of GhANN4 and GhANN11 revealed that, compared with those of the controls, the leaf wilting of GhANN4-silenced plants was more obvious, and the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were lower under NaCl and PEG stress. Moreover, the expression of stress marker genes (GhCBL3, GhDREB2A, GhDREB2C, GhPP2C, GhRD20-2, GhCIPK6, GhNHX1, GhRD20-1, GhSOS1, GhSOS2 and GhSnRK2.6) was significantly downregulated in GhANN4-silenced plants after stress. Under cold stress, the growth of the GHANN11-silenced plants was significantly weaker than that of the control plants, and the activities of POD, SOD, and CAT were also lower. However, compared with those of the control, the elasticity and orthostatic activity of the GhANN11-silenced plants were greater; the POD, SOD, and CAT activities were higher; and the GhDREB2C, GhHSP, and GhSOS2 expression levels were greater under heat stress. These results suggest that different GhANN family members respond differently to different types of abiotic stress. Full article
(This article belongs to the Section Molecular Plant Sciences)
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21 pages, 5200 KiB  
Article
The Silencing of GhPIP5K2 and GhPIP5K22 Weakens Abiotic Stress Tolerance in Upland Cotton (Gossypium hirsutum)
by Pingjie Ling, Jisheng Ju, Xueli Zhang, Wei Wei, Jin Luo, Ying Li, Han Hai, Bowen Shang, Hongbo Cheng, Caixiang Wang, Xianliang Zhang and Junji Su
Int. J. Mol. Sci. 2024, 25(3), 1511; https://doi.org/10.3390/ijms25031511 - 26 Jan 2024
Cited by 2 | Viewed by 1606
Abstract
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks), essential enzymes in the phosphatidylinositol signaling pathway, are crucial for the abiotic stress responses and the overall growth and development of plants. However, the GhPIP5Ks had not been systematically studied, and their function in upland cotton was unknown. This [...] Read more.
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks), essential enzymes in the phosphatidylinositol signaling pathway, are crucial for the abiotic stress responses and the overall growth and development of plants. However, the GhPIP5Ks had not been systematically studied, and their function in upland cotton was unknown. This study identified a total of 28 GhPIP5Ks, and determined their chromosomal locations, gene structures, protein motifs and cis-acting elements via bioinformatics analysis. A quantitative real-time PCR (qRT‒PCR) analysis showed that most GhPIP5Ks were upregulated under different stresses. A virus-induced gene silencing (VIGS) assay indicated that the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were significantly decreased, while malondialdehyde (MDA) content were significantly increased in GhPIP5K2- and GhPIP5K22-silenced upland cotton plants under abiotic stress. Furthermore, the expression of the stress marker genes GhHSFB2A, GhHSFB2B, GhDREB2A, GhDREB2C, GhRD20-1, GhRD29A, GhBIN2, GhCBL3, GhNHX1, GhPP2C, GhCBF1, GhSnRK2.6 and GhCIPK6 was significantly decreased in the silenced plants after exposure to stress. These results revealed that the silencing of GhPIP5K2 and GhPIP5K22 weakened the tolerance to abiotic stresses. These discoveries provide a foundation for further inquiry into the actions of the GhPIP5K gene family in regulating the response and resistance mechanisms of cotton to abiotic stresses. Full article
(This article belongs to the Section Molecular Plant Sciences)
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14 pages, 5352 KiB  
Article
Arabidopsis Protein Phosphatase PIA1 Impairs Plant Drought Tolerance by Serving as a Common Negative Regulator in ABA Signaling Pathway
by Yan Huang, Rongqian Yang, Huiling Luo, Yuan Yuan, Zhihong Diao, Junhao Li, Shihe Gong, Guozhi Yu, Huipeng Yao, Huaiyu Zhang and Yi Cai
Plants 2023, 12(14), 2716; https://doi.org/10.3390/plants12142716 - 21 Jul 2023
Cited by 5 | Viewed by 2037
Abstract
Reversible phosphorylation of proteins is a ubiquitous regulatory mechanism in vivo that can respond to external changes, and plays an extremely important role in cell signal transduction. Protein phosphatase 2C is the largest protein phosphatase family in higher plants. Recently, it has been [...] Read more.
Reversible phosphorylation of proteins is a ubiquitous regulatory mechanism in vivo that can respond to external changes, and plays an extremely important role in cell signal transduction. Protein phosphatase 2C is the largest protein phosphatase family in higher plants. Recently, it has been found that some clade A members can negatively regulate ABA signaling pathways. However, the functions of several subgroups of Arabidopsis PP2C other than clade A have not been reported, and whether other members of the PP2C family also participate in the regulation of ABA signaling pathways remains to be studied. In this study, based on the previous screening and identification work of PP2C involved in the ABA pathway, the clade F member PIA1 encoding a gene of the PP2C family, which was down-regulated after ABA treatment during the screening, was selected as the target. Overexpression of PIA1 significantly down-regulated the expression of ABA marker gene RD29A in Arabidopsis protoplasts, and ABA-responsive elements have been found in the cis-regulatory elements of PIA1 by promoter analysis. When compared to Col-0, transgenic plants overexpressing PIA1 were less sensitive to ABA, whereas pia1 showed the opposite trait in seed germination, root growth, and stomatal opening experiments. Under drought stress, SOD, POD, CAT, and APX activities of PIA1 overexpression lines were lower than Col-0 and pia1, while the content of H2O2 was higher, leading to its lowest survival rate in test plants, which were consistent with the significant inhibition of the expression of ABA-dependent stress-responsive genes RD29B, ABI5, ABF3, and ABF4 in the PIA1 transgenic background after ABA treatment. Using yeast two-hybrid and luciferase complementation assays, PIA1 was found to interact with multiple ABA key signaling elements, including 2 RCARs and 6 SnRK2s. Our results indicate that PIA1 may reduce plant drought tolerance by functioning as a common negative regulator involved in ABA signaling pathway. Full article
(This article belongs to the Special Issue The Role of Abscisic Acid (ABA) Machinery in Stress Response)
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14 pages, 2600 KiB  
Article
Barkhausen Noise Emission as a Function of Tensile Stress in Low-Alloyed Steels: Influence of Corrosion and Steel Strength
by František Bahleda, Miroslav Neslušan, Filip Pastorek, Radoslav Koňár and Tibor Kubjatko
Appl. Sci. 2023, 13(11), 6574; https://doi.org/10.3390/app13116574 - 29 May 2023
Cited by 10 | Viewed by 1529
Abstract
Components of civil buildings are frequently made of low-alloyed steel, which can suffer from corrosion damage. This damage reduces their bearing capacity and/or redistributes the load to neighboring regions with the potential risk of their collapse. For this reason, this study deals with [...] Read more.
Components of civil buildings are frequently made of low-alloyed steel, which can suffer from corrosion damage. This damage reduces their bearing capacity and/or redistributes the load to neighboring regions with the potential risk of their collapse. For this reason, this study deals with the non-destructive monitoring of bodies made of these steels based on Barkhausen noise emission. The superimposing contribution of corrosion extent and exerted tensile load is investigated on samples of variable yield strength in the range from 235 to 1100 MPa. It is found that the presence of a corroded layer attenuates Barkhausen noise and makes the body harder from a magnetic point of view. The reduced effective thickness of the samples as a result of corrosion damage increases the true stress. Barkhausen noise grows along with the tensile stress in the direction of exerted stress at the expense of decreasing Barkhausen noise in the perpendicular direction. The evolution of Barkhausen noise versus tensile stress is mostly shifted to the lower values of Barkhausen noise, along with the increasing degree of corrosion damage. The evolution of Barkhausen noise versus tensile stress is also affected by the initial microstructure and the corresponding yield strength of the low-alloyed steel. Corrosion attack results in the growth of FWHM, which is compensated by the decreasing evolution along with the tensile stresses. The effective values drop down with the higher extent of corrosion damage. However, the response with respect to the tensile stress is asymmetric in RD and TD due to the realignment of DWs into RD. Finally, PP tends to increase with the corrosion attack as well as the tensile stress and this parameter only exhibits the systematic behavior in RD as well as TD. On the other hand, MBN-extracted parameters as well as their combination provide no exclusive values on which the pure contribution of corrosion and tensile stress can be distinguished. Full article
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26 pages, 26570 KiB  
Article
PP19128R, a Multiepitope Vaccine Designed to Prevent Latent Tuberculosis Infection, Induced Immune Responses In Silico and In Vitro Assays
by Fan Jiang, Cong Peng, Peng Cheng, Jie Wang, Jianqi Lian and Wenping Gong
Vaccines 2023, 11(4), 856; https://doi.org/10.3390/vaccines11040856 - 17 Apr 2023
Cited by 23 | Viewed by 2994
Abstract
Background: Latent tuberculosis infection (LTBI) is the primary source of active tuberculosis (ATB), but a preventive vaccine against LTBI is lacking. Methods: In this study, dominant helper T lymphocyte (HTL), cytotoxic T lymphocyte (CTL), and B-cell epitopes were identified from nine antigens related [...] Read more.
Background: Latent tuberculosis infection (LTBI) is the primary source of active tuberculosis (ATB), but a preventive vaccine against LTBI is lacking. Methods: In this study, dominant helper T lymphocyte (HTL), cytotoxic T lymphocyte (CTL), and B-cell epitopes were identified from nine antigens related to LTBI and regions of difference (RDs). These epitopes were used to construct a novel multiepitope vaccine (MEV) based on their antigenicity, immunogenicity, sensitization, and toxicity. The immunological characteristics of the MEV were analyzed with immunoinformatics technology and verified by enzyme-linked immunospot assay and Th1/Th2/Th17 cytokine assay in vitro. Results: A novel MEV, designated PP19128R, containing 19 HTL epitopes, 12 CTL epitopes, 8 B-cell epitopes, toll-like receptor (TLR) agonists, and helper peptides, was successfully constructed. Bioinformatics analysis showed that the antigenicity, immunogenicity, and solubility of PP19128R were 0.8067, 9.29811, and 0.900675, respectively. The global population coverage of PP19128R in HLA class I and II alleles reached 82.24% and 93.71%, respectively. The binding energies of the PP19128R-TLR2 and PP19128R-TLR4 complexes were −1324.77 kcal/mol and −1278 kcal/mol, respectively. In vitro experiments showed that the PP19128R vaccine significantly increased the number of interferon gamma-positive (IFN-γ+) T lymphocytes and the levels of cytokines, such as IFN-γ, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-10. Furthermore, positive correlations were observed between PP19128R-specific cytokines in ATB patients and individuals with LTBI. Conclusions: The PP19128R vaccine is a promising MEV with excellent antigenicity and immunogenicity and no toxicity or sensitization that can induce robust immune responses in silico and in vitro. This study provides a vaccine candidate for the prevention of LTBI in the future. Full article
(This article belongs to the Special Issue Research Progress of New Tuberculosis Vaccines and Vaccine Design)
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16 pages, 1590 KiB  
Article
Changes of Hemodynamic Parameters after Intradialytic Glucose Injection
by Longin Niemczyk, Katarzyna Romejko, Katarzyna Szamotulska, Daniel Schneditz and Stanisław Niemczyk
Nutrients 2023, 15(2), 437; https://doi.org/10.3390/nu15020437 - 14 Jan 2023
Cited by 3 | Viewed by 2727
Abstract
Background: Intradialytic hypotension (IDH) is a frequent complication of hemodialysis (HD). Current methods of IDH prevention are insufficient. Methods: We analyzed the intradialytic time course of systolic (SBP), diastolic (DBP), mean arterial (MAP), pulse pressure (PP), and heart rate (HR) in a group [...] Read more.
Background: Intradialytic hypotension (IDH) is a frequent complication of hemodialysis (HD). Current methods of IDH prevention are insufficient. Methods: We analyzed the intradialytic time course of systolic (SBP), diastolic (DBP), mean arterial (MAP), pulse pressure (PP), and heart rate (HR) in a group of chronic kidney disease (CKD) patients. First, 30 min into HD, a 40% glucose solution was injected into the venous line of the extracorporeal circulation at a dose of 0.5 g/kg of dry weight. Pressures and HR were measured in frequent intervals. Relative volume overload was determined by bioimpedance spectroscopy. Results: Thirty-five participants were studied. SBP increased after 5, 10, and 20 min of glucose infusion. DBP increased after 2 and 3 h and also at the end of HD. PP increased after 5, 10, and 20 min of glucose infusion and fell after the 2nd and 3rd hour and also at the end of HD. MAP increased after 2 and 3 h of glucose injection and at the end of HD. Significant interactions of the time course of SBP, DBP, MAP, with HR at baseline and of the time course of PP with fluid overload were observed. Symptomatic hypotensive episodes were absent. Conclusions: Glucose infusions during HD prevent symptomatic IDH and do not cause severe hypertensive episodes. Full article
(This article belongs to the Special Issue Hydration Status and Cardiovascular Diseases)
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7 pages, 920 KiB  
Article
Open Globe Injury (OGI) with a Presence of an Intraocular Foreign Body (IOFB)—Epidemiology, Management, and Risk Factors in Long Term Follow-Up
by Michał Jabłoński, Mateusz Winiarczyk, Katarzyna Biela, Paweł Bieliński, Monika Jasielska, Joanna Batalia and Jerzy Mackiewicz
J. Clin. Med. 2023, 12(1), 190; https://doi.org/10.3390/jcm12010190 - 26 Dec 2022
Cited by 7 | Viewed by 1827
Abstract
The purpose of the study was to evaluate visual outcomes and consider management strategies in the eyes with an intraocular foreign body (IOFB). In a single-center, retrospective case-control study, 36 eyes of 36 patients who suffered from open globe injury (OGI) with IOFB [...] Read more.
The purpose of the study was to evaluate visual outcomes and consider management strategies in the eyes with an intraocular foreign body (IOFB). In a single-center, retrospective case-control study, 36 eyes of 36 patients who suffered from open globe injury (OGI) with IOFB were admitted to the Department of Vitreoretinal Surgery of Medical University of Lublin, Poland from January 2015 to December 2020. Most frequent primary procedure was the pars plana vitrectomy (PPV) with IOFB removal (n = 28). Retinal detachment (RD) developed in nine eyes soon after injury or as a further complication. Recurrent retinal detachment occurred in eight of these nine cases. Final VA 0.1 or better was observed in 21 eyes (58%). Fifteen patients had BCVA of less than 0.1. One eye was not included in the final VA assessment due to the short follow-up period. In 25 out of 28 patients who underwent any kind of pars plana vitrectomy (ppV) a BCVA of <0.4 was observed. The prognosis after an IOFB injury is uncertain due to multiple factors in a peri- and postoperative period. Factors predisposing to poor visual outcomes are: IOFB localization in the posterior segment, retinal detachment, vitreous hemorrhage and prolonged silicone oil tamponade. Full article
(This article belongs to the Special Issue Advances in Vitreoretinal Interventions for Eye Diseases)
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18 pages, 4707 KiB  
Article
Identification of Peanut AhMYB44 Transcription Factors and Their Multiple Roles in Drought Stress Responses
by Yonghui Liu, Yue Shen, Man Liang, Xuyao Zhang, Jianwen Xu, Yi Shen and Zhide Chen
Plants 2022, 11(24), 3522; https://doi.org/10.3390/plants11243522 - 14 Dec 2022
Cited by 12 | Viewed by 2331
Abstract
MYB transcription factors (TFs) comprise a large gene family that plays an important role in plant growth, development, stress responses, and defense regulation. However, their functions in peanut remain to be further elucidated. Here, we identified six AhMYB44 genes (AhMYB44-01/11, AhMYB44-05/15 [...] Read more.
MYB transcription factors (TFs) comprise a large gene family that plays an important role in plant growth, development, stress responses, and defense regulation. However, their functions in peanut remain to be further elucidated. Here, we identified six AhMYB44 genes (AhMYB44-01/11, AhMYB44-05/15, and AhMYB44-06/16) in cultivated peanut. They are typical R2R3-MYB TFs and have many similarities but different expression patterns in response to drought stress, suggesting different functions under drought stress. Homologous genes with higher expression in each pair were selected for further study. All of them were nuclear proteins and had no self-transactivation activity. In addition, we compared the performances of different lines at germination, seedling, and adult stages under drought stress. After drought treatment, the overexpression of AhMYB44-11 transgenic plants resulted in the longest root length at the seedling stage. Levels of proline, soluble sugar and chlorophyll, and expression levels of stress-related genes, including P5CS1, RD29A, CBF1, and COR15A, were higher than those of the wild type (WT) at the adult stage. While the overexpression of AhMYB44-16 significantly increased the drought sensitivity of plants at all stages, with differential ABA content, the expression levels of the ABA-related genes PP2CA and ABI1 were significantly upregulated and those of ABA1 and ABA2 were significantly downregulated compared with the WT. AhMYB44-05 showed similar downregulated expression as AhMYB44-16 under drought stress, but its overexpression in Arabidopsis did not significantly affect the drought resistance of transgenic plants. Based on the results, we propose that AhMYB44-11 plays a role as a positive factor in drought tolerance by increasing the transcription abundance of stress-related genes and the accumulation of osmolytes, while AhMYB44-16 negatively regulates drought tolerance through its involvement in ABA-dependent stress response pathways. Full article
(This article belongs to the Special Issue The Trade-Offs between Growth and Development and Stress in Plants)
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13 pages, 1957 KiB  
Article
Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis
by Yushi Jiang, Yuzhe Zhang, Ruijie Duan, Jiayi Fan, Peng Jiao, Hongji Sun, Shuyan Guan and Siyan Liu
Agronomy 2022, 12(12), 2948; https://doi.org/10.3390/agronomy12122948 - 24 Nov 2022
Cited by 10 | Viewed by 2341
Abstract
Drought stress critically endangers the growth and development of crops. Glutathione S-transferase plays a vital role in response to abiotic stress. However, there are few studies on the role of glutathione S-transferase in maize drought stress. In this study, the significantly downregulated expression [...] Read more.
Drought stress critically endangers the growth and development of crops. Glutathione S-transferase plays a vital role in response to abiotic stress. However, there are few studies on the role of glutathione S-transferase in maize drought stress. In this study, the significantly downregulated expression of ZmGST26 in roots under drought stress was analyzed by qRT-PCR. Promoter analyses showed that there were several cis-acting elements related to drought stress and that were involved in oxidative response in the promoter region of ZmGST26. Subcellular localization results showed that ZmGST26 was localized in the nucleus. The transgenic lines of the Arabidopsis over-expressing ZmGST26 were more sensitive to drought stress and ABA in seed germination and inhibited ABA-mediated stomatal closure. Under drought stress, phenotypic analyses showed that the germination rate, root length and survival rate of ZmGST26 overexpressing lines were significantly lower than those of wild-type lines. The determination of physiological and biochemical indexes showed that the water loss rate, malondialdehyde, O2 and H2O2 of the overexpression lines significantly increased compared with wild-type Arabidopsis, but the antioxidant enzyme activities (CAT, SOD and POD), and proline and chlorophyll contents were significantly reduced. Subsequently, the qRT-PCR analysis of drought stress-related gene expression showed that, under drought stress conditions, the expression levels of DREB2A, RD29A, RD29B and PP2CA genes in ZmGST26 overexpression lines were significantly lower than those in wild-type Arabidopsis. In summary, ZmGST26 reduced the drought resistance of plants by aggravating the accumulation of reactive oxygen species in Arabidopsis. Full article
(This article belongs to the Topic Tolerance to Drought and Salt Stress in Plants)
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26 pages, 8832 KiB  
Article
A Novel R2R3-MYB Transcription Factor FtMYB22 Negatively Regulates Salt and Drought Stress through ABA-Dependent Pathway
by Haixia Zhao, Panfeng Yao, Jiali Zhao, Huala Wu, Shuang Wang, Ying Chen, Mufan Hu, Tao Wang, Chenglei Li and Qi Wu
Int. J. Mol. Sci. 2022, 23(23), 14549; https://doi.org/10.3390/ijms232314549 - 22 Nov 2022
Cited by 26 | Viewed by 3561
Abstract
Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse cereal with strongly abiotic resistance. The MYB family plays a regulatory role in plant growth, development, and responses to biotic and abiotic stresses. However, the characteristics and regulatory mechanisms of MYB transcription factors in [...] Read more.
Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse cereal with strongly abiotic resistance. The MYB family plays a regulatory role in plant growth, development, and responses to biotic and abiotic stresses. However, the characteristics and regulatory mechanisms of MYB transcription factors in Tartary buckwheat remain unclarified. Here, this study cloned the FtMYB22 gene from Tartary buckwheat, and investigated its involvement in responding to individual water deficit and salt stress in Arabidopsis. Sequence analysis highlighted that the N-termini of FtMYB22 contained two highly conserved SANT domains and one conserved domain from the SG20 subfamily. Nucleus-localized FtMYB22 did not have individual transcriptional activation activity. Water deficiency and salt stress induced the high expression of the GUS gene, which was driven by the promoter of FtMYB22. Yeast stress experiments showed that the overexpression of FtMYB22 significantly reduced the growth activity of transgenic yeast under water deficit or salt stress. Consistently, the overexpression of FtMYB22 reduced the salt and water deficit stress resistance of the transgenic plants. In addition, physiological parameters showed that transgenic plants had lower proline and antioxidant enzyme activity under stress conditions. Compared to the wild-type (WT), transgenic plants accumulated more malondialdehyde (MDA), H2O2, and O2; they also showed higher ion permeability and water loss rates of detached leaves under stress treatments. Notably, FtMYB22 was involved in plant stress resistance through an ABA-dependent pathway. Under stress conditions, the expression of RD29A, RD29B, PP2CA, KIN1, COR15A, and other genes in response to plant stress in transgenic lines was significantly lower than that in the WT (p < 0.05). Furthermore, yeast two-hybrid assay showed that there was a significant interaction between FtMYB22 and the ABA receptor protein RCAR1/2, which functioned in the ABA signal pathway. Altogether, FtMYB22, as a negative regulator, inhibited a variety of physiological and biochemical reactions, affected gene expression and stomatal closure in transgenic plants through the ABA-dependent pathway, and reduced the tolerance of transgenic Arabidopsis to water deficiency and salt stress. Based on these fundamental verifications, further studies would shed light on the hormone signal response mechanism of FtMYB22. Full article
(This article belongs to the Special Issue Crop Stress Biology and Molecular Breeding 2.0)
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13 pages, 2179 KiB  
Article
Deep Learning for the Automatic Quantification of Pleural Plaques in Asbestos-Exposed Subjects
by Ilyes Benlala, Baudouin Denis De Senneville, Gael Dournes, Morgane Menant, Celine Gramond, Isabelle Thaon, Bénédicte Clin, Patrick Brochard, Antoine Gislard, Pascal Andujar, Soizick Chammings, Justine Gallet, Aude Lacourt, Fleur Delva, Christophe Paris, Gilbert Ferretti, Jean-Claude Pairon and François Laurent
Int. J. Environ. Res. Public Health 2022, 19(3), 1417; https://doi.org/10.3390/ijerph19031417 - 27 Jan 2022
Cited by 4 | Viewed by 2623
Abstract
Objective: This study aimed to develop and validate an automated artificial intelligence (AI)-driven quantification of pleural plaques in a population of retired workers previously occupationally exposed to asbestos. Methods: CT scans of former workers previously occupationally exposed to asbestos who participated in the [...] Read more.
Objective: This study aimed to develop and validate an automated artificial intelligence (AI)-driven quantification of pleural plaques in a population of retired workers previously occupationally exposed to asbestos. Methods: CT scans of former workers previously occupationally exposed to asbestos who participated in the multicenter APEXS (Asbestos PostExposure Survey) study were collected retrospectively between 2010 and 2017 during the second and the third rounds of the survey. A hundred and forty-one participants with pleural plaques identified by expert radiologists at the 2nd and the 3rd CT screenings were included. Maximum Intensity Projection (MIP) with 5 mm thickness was used to reduce the number of CT slices for manual delineation. A Deep Learning AI algorithm using 2D-convolutional neural networks was trained with 8280 images from 138 CT scans of 69 participants for the semantic labeling of Pleural Plaques (PP). In all, 2160 CT images from 36 CT scans of 18 participants were used for AI testing versus ground-truth labels (GT). The clinical validity of the method was evaluated longitudinally in 54 participants with pleural plaques. Results: The concordance correlation coefficient (CCC) between AI-driven and GT was almost perfect (>0.98) for the volume extent of both PP and calcified PP. The 2D pixel similarity overlap of AI versus GT was good (DICE = 0.63) for PP, whether they were calcified or not, and very good (DICE = 0.82) for calcified PP. A longitudinal comparison of the volumetric extent of PP showed a significant increase in PP volumes (p < 0.001) between the 2nd and the 3rd CT screenings with an average delay of 5 years. Conclusions: AI allows a fully automated volumetric quantification of pleural plaques showing volumetric progression of PP over a five-year period. The reproducible PP volume evaluation may enable further investigations for the comprehension of the unclear relationships between pleural plaques and both respiratory function and occurrence of thoracic malignancy. Full article
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7 pages, 988 KiB  
Communication
Complete Genome Sequence of Macrobrachium rosenbergii Golda Virus (MrGV) from China
by Fanzeng Meng, Yiting Wang, Guohao Wang, Tao Hu, La Xu, Kathy F. J. Tang, Weifeng Shi, Fan Zhang, Xuan Dong and Jie Huang
Animals 2022, 12(1), 27; https://doi.org/10.3390/ani12010027 - 23 Dec 2021
Cited by 3 | Viewed by 4165
Abstract
In a meta-transcriptome study of the giant freshwater prawn Macrobrachium rosenbergii sampled in 2018 from a hatchery, we identified a variant of Macrobrachium rosenbergii golda virus (MrGV) in postlarvae without clinical signs. The virus belongs to the family Roniviridae, and the genome [...] Read more.
In a meta-transcriptome study of the giant freshwater prawn Macrobrachium rosenbergii sampled in 2018 from a hatchery, we identified a variant of Macrobrachium rosenbergii golda virus (MrGV) in postlarvae without clinical signs. The virus belongs to the family Roniviridae, and the genome of this MrGV variant, Mr-18, consisted of 28,957 nucleotides, including 4 open reading frames (ORFs): (1) ORF1a, encoding a 3C-like protein (3CLP) (4933 aa); (2) ORF1b, encoding a replicase polyprotein (2877 aa); (3) ORF2, encoding a hypothetical nucleocapsid protein (125 aa); and (4) ORF3, encoding a glycoprotein (1503 aa). ORF1a overlaps with ORF1b with 40 nucleotides, where a −1 ribosomal frameshift with slippage sequence 5′-G14925GGUUUU14931-3′ produces the pp1ab polyprotein. The genomic sequence of Mr-18 shared 97.80% identity with MrGV LH1-2018 discovered in Bangladesh. The amino acid sequence identities between them were 99.30% (ORF1a), 99.60% (ORF1b), 100.00% (ORF2), and 99.80% (ORF3), respectively. Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) proteins revealed that they clustered together and formed a separate cluster from the genus Okavirus. The finding of MrGV in China warrants further studies to determine its pathogenicity and prevalence within the region. Full article
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14 pages, 1976 KiB  
Article
Retinal Changes before and after Silicone Oil Removal in Eyes with Rhegmatogenous Retinal Detachment Using Swept-Source Optical Coherence Tomography
by Jungwook Lee, Heeyoon Cho, Minho Kang, Rimkyung Hong, Mincheol Seong and Yongun Shin
J. Clin. Med. 2021, 10(22), 5436; https://doi.org/10.3390/jcm10225436 - 21 Nov 2021
Cited by 20 | Viewed by 4052
Abstract
This study aimed to evaluate and compare the retinal and choroidal thickness and vessel density (VD) changes between silicone oil (SO) tamponade and after SO removal using swept-source optical coherence tomography (SS-OCT) and OCT angiography (OCTA). Thirty patients who underwent pars plana vitrectomy [...] Read more.
This study aimed to evaluate and compare the retinal and choroidal thickness and vessel density (VD) changes between silicone oil (SO) tamponade and after SO removal using swept-source optical coherence tomography (SS-OCT) and OCT angiography (OCTA). Thirty patients who underwent pars plana vitrectomy for retinal detachment (RD) with SO tamponade were included. SS-OCT and OCTA were conducted before RD surgery, during SO tamponade, and after SO removal. A 3-dimensional volumetric wide scan protocol was used for the analysis. The segmented retina, choroidal thickness map, and peripapillary thickness were then measured. For the OCTA analysis, 4.5 × 4.5 mm scans were used. Superficial and deep capillary plexus VDs in unaffected fellow eyes and eyes after SO removal were compared. During the SO tamponade period, the thickness of the parafoveal total retina, ganglion cell-inner plexiform layer, and peripapillary retinal nerve fiber layer (ppRNFL) were significantly thinner than those of unaffected fellow eyes (p < 0.05). The parafoveal layer thickness thinning recovered up to three to six months after SO removal. Moreover, six months after SO removal, the parafoveal thickness was not significantly different compared to that of unaffected fellow eyes (p > 0.05). However, the ppRNFL thickness was significantly decreased during SO tamponade and remained unrecovered six months after SO removal. There was no significant difference in the VD on the OCTA. Thus, SO tamponade and removal for RD resulted in a change in the retinal and peripapillary thickness. This may be due to the mechanical pressure effect of SO. Full article
(This article belongs to the Section Ophthalmology)
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