Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Drought Stress Induced the Downregulated Expression of ZmGST26 Gene
2.2. Subcellular Localization
2.3. Overexpression of ZmGST26 Reduced the Drought Tolerance of Transgenic Arabidopsis
2.4. Overexpression of ZmGST26 Increased the Sensitivity of Transgenic Arabidopsis to Drought
2.5. ZmGST26 Negatively Regulates the Expression of Drought-Related Genes in Overexpressed Arabidopsis
3. Materials and Methods
3.1. Plant Materials and Growth Conditions
3.2. Sequence Analysis
3.3. RNA Extraction and qRT-PCR Analysis
3.4. Subcellular Localization
3.5. Acquisition and Phenotypic Analysis of Overexpressed Arabidopsis
3.6. Determination of O2−, H2O2 and Physiological and Biochemical Indexes in Overexpressed Arabidopsis
3.7. Guard Cell Stomatal Aperture Observations
3.8. Statistical Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jiang, Y.; Zhang, Y.; Duan, R.; Fan, J.; Jiao, P.; Sun, H.; Guan, S.; Liu, S. Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis. Agronomy 2022, 12, 2948. https://doi.org/10.3390/agronomy12122948
Jiang Y, Zhang Y, Duan R, Fan J, Jiao P, Sun H, Guan S, Liu S. Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis. Agronomy. 2022; 12(12):2948. https://doi.org/10.3390/agronomy12122948
Chicago/Turabian StyleJiang, Yushi, Yuzhe Zhang, Ruijie Duan, Jiayi Fan, Peng Jiao, Hongji Sun, Shuyan Guan, and Siyan Liu. 2022. "Overexpression of Maize Glutathione S-Transferase ZmGST26 Decreases Drought Resistance of Arabidopsis" Agronomy 12, no. 12: 2948. https://doi.org/10.3390/agronomy12122948