Search Results (645)

Background: With the evolving paradigm of vaccine development, microcapsules have attracted considerable research interest as particulate adjuvants over the past decades. However, the rational engineering design of microcapsule-based composite adjuvant systems to elicit robust immune responses remains a significant challenge. Methods: This study developed polylactic acid (PLA) microcapsules with spatiotemporally coupled delivery and immunopotentiator properties. The resulting formulations were assessed for humoral and cellular immune responses in mice. Results: We prepared uniform-sized microcapsules (MC) and formulated them with monophosphoryl lipid A (MPLA) as a composite component (MPLA@MC), with hydrodynamic diameters of 4.58 μm and 4.12 μm, respectively. Such composite adjuvants, when loaded with ovalbumin (OVA) to form OVA@MC and OVA&MPLA@MC, promoted cellular uptake and activation, exhibiting preferred lysosomal escape advantages. For in vivo experiments, microcapsule-based vaccines elevated serum levels of IgG antibody, and OVA&MPLA@MC induced Th1-biased antibody responses. Specifically, OVA&MPLA@MC also elicited strong cellular immune responses compared to other vaccines, as evidenced by increased secretion of Interferon-γ (IFN-γ) in mouse splenocytes and Granzyme B (Gzmb) in T cells. Mechanistically, muscle tissues at the injection site showed that microcapsule-based vaccines enhanced the recruitment for phagocytosis. Meanwhile, bulk RNA sequencing (RNA-seq) confirmed extensive activation of immune responses and related signaling pathways. Conclusions: This rationally designed composite strategy for spatiotemporally coupled delivery serves as a potent platform for orchestrating synergistic immune responses, opening up new avenues for the development of effective therapeutic and anti-infectious vaccines. Full article

Maleic anhydride (MAH) grafting is widely employed to compatibilise polylactic acid (PLA) in fibre-reinforced composites; however, the influence of reactant addition sequence during melt processing varies widely across the literature, with no clear consensus on an optimal approach. In this study, the effect of reactant addition sequence on the graft yield of MAH onto PLA was investigated using dicumyl peroxide (DCP) as an initiator. Four loading protocols were examined in which the order of addition of PLA, DCP, and MAH was varied using approaches commonly reported in the literature, while all other processing conditions were held constant. A strong dependence of grafting yield on addition sequence was observed, with values ranging from 0.12% to 0.51%, corresponding to more than a four-fold variation under otherwise identical processing conditions. Simultaneous addition of PLA, DCP, and MAH produced the highest grafting yield, attributed to a more effective utilisation of peroxide-derived radicals. These results demonstrate that the reactant addition sequence is a critical processing variable governing MAH grafting efficiency and that simultaneous addition represents the most effective processing strategy under the conditions examined. Full article

Neurotoxicity caused by snake venom phospholipase A₂ (PLA₂) activity derived from coral snakes (e.g., Micrurus tener, Micrurus fulvius, group IA PLA₂) and some rattlesnakes (e.g., Crotalus scutulatus, group IIA PLA₂) is medically significant. Of interest, the catalytic site of PLA₂ also binds to activated clotting factor X, causing anticoagulation. Given that ruthenium (Ru)-containing compounds have been demonstrated to inactivate hemotoxic venoms in a solvent-dependent manner (e.g., 0.9% NaCl, phosphate-buffered saline), we wished to determine if RuCl₃ would cause solvent-dependent inhibition of snake venom group IA and group IIA PLA₂ in human plasma with thrombelastography. It was determined that RuCl₃ significantly decreased the anticoagulant effects of group IA PLA₂ derived from M. tener and M. fulvius venoms in the presence of 0.9% NaCl, but not phosphate-buffered saline. In contrast, group IIA PLA₂ anticoagulant activity derived from C. scutulatus venom was inhibited by RuCl₃ in both solvents. It is concluded that the different ions formed by RuCl₃ in different solvents may interact with novel disulfide bridges unique to group IA and IIA PLA₂ or through some other mechanism. In vivo validation of Ru-based enzyme inhibitor effects on neurotoxicity associated with either group IA or IIA remains a critical translational issue. Full article

Additive manufacturing enables the rapid fabrication of radiographic phantoms for X-ray and CT imaging, supporting applications such as patient simulation, dosimetry, imaging protocol optimization, and quality assurance. Polylactic acid (PLA) and acrylonitrile butadiene styrene (ABS) are among the most widely used printing polymers in phantoms; however, their X-ray attenuation properties can vary substantially among manufacturers, product lines within manufacturers, and even between colors of the same product. Cylindrical samples of 34 PLA filaments from 11 manufacturers and 13 ABS filaments from 9 manufacturers were evaluated for X-ray attenuation and energy dependence between 70 and 140 kV using a clinical CT scanner. Measured mass densities ranged from 1.17 to 1.34 g/cm³ for PLA and 1.03–1.11 g/cm³ for ABS. At 120 kV, Hounsfield unit (HU) values spanned 109 to 424 HU for PLA and −34 to 40 HU for ABS. Energy dependence, quantified as the HU at 70 kV minus HU at 140 kV, ranged from −29 to +172 HU for PLA filaments and −52 to −4 HU for ABS filaments. Identical products differing only in color showed HU variations from <2 HU to >90 HU at 120 kV, with no consistent pattern linking specific colors to highest or lowest attenuation. These findings demonstrate that 3D printing materials require individual characterization, as base polymer designation alone does not predict X-ray behavior accurately. The observed variability, however, enables the design of phantoms with tailored attenuation and energy-dependent contrast. Referring only to base polymers when specifying 3D printing materials for radiographic phantoms or suggesting printing materials as radiographic substitutes to mimic a specified tissue or reference material without naming the actual product, including color, is, thus, insufficient. Full article

Background/Objective: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a highly prevalent chronic liver disease with no specific therapeutics. Wuling Powder (WLP) is a classic traditional Chinese medicine prescription with therapeutic potential against MASLD, yet its molecular mechanism remains unclear. This study aims to elucidate the mechanism and possible effective substances of WLP in the treatment of MASLD. Methods: A rat MASLD model was established via high-fat diet feeding to evaluate WLP’s efficacy. Untargeted metabolomics and 16S rRNA sequencing were used to explore the effects of WLP on metabolism and gut microbiota in vivo. Serum pharmacochemistry combined with metabolomics was used to analyze the key active components and core targets of WLP against MASLD, and molecular docking and cell experiments were used to verify the relationship between them. Results: WLP reduced hepatic lipid accumulation and pathological damage, improved lipid levels in blood liver, enhanced antioxidant capacity, and alleviated inflammation in MASLD rats. Mechanistically, WLP regulated 19 metabolic pathways. It also decreased the Firmicutes/Bacteroidota ratio and reduced the abundance of potential pathogenic bacteria (Romboutsia and Turicibacter). Thirty-one WLP-derived components were identified in serum, 13 of which were key active components for treating MASLD. These components, especially 11-deoxyalisol A and 8β-methoxyatractylenolide I, alleviated hepatic steatosis by downregulating NOS2 and PLA2G2A expression. Conclusions: The alleviation of MASLD by WLP was mediated by the regulation of 8 metabolic pathways, alterations in the abundance of Romboutsia and Turicibacter, and the restoration of 20 metabolite levels, an effect primarily ascribed to 13 distinct pharmacodynamic components derived from WLP. Full article

Foodborne pathogens cause hundreds of millions of illnesses annually, underscoring the urgent need for advanced antimicrobial food packaging materials. The objective of this study was to develop a crosslinked cyclodextrin metal–organic framework, loaded with gold nanoparticles (CL-AuNPs@CD-MOF) and integrated into a PLA-Zein composite film with humidity-responsive antimicrobial release, as a sustainable and high-performance packaging solution to address the critical limitations of conventional materials in controlling microbial contamination during food storage. Therefore, gold nanoparticles (AuNPs) were synthesized via a green approach using CD-MOFs as stabilizers and p-coumaric acid as a natural reducing agent, then crosslinked with diphenyl carbonate (DPC) to produce CL-AuNPs@CD-MOF. Crosslinking conditions were optimized to a CD-MOF:DPC ratio of 1:1, 1080 min reaction time, and 80 °C, preserving the cubic morphology and crystalline structure while transforming burst release into sustained antimicrobial activity against E. coli and S. aureus over 7 days. Then, the incorporation of CL-AuNPs@CD-MOF into PLA-Zein films yielded a composite packaging material with favorable mechanical and barrier properties, including a water vapor transmission rate of 539.44 g/m²·24 h and an oxygen permeability of 235.90 cm³/m²·24 h·0.1 MPa. Progressive elimination of E. coli, S. aureus, and L. monocytogenes over 7 days was confirmed, with antimicrobial efficacy originating exclusively from the CL-AuNPs@CD-MOF component. Application on Agaricus bisporus over 12 days of refrigerated storage demonstrated superior preservation performance: mushrooms inoculated with L. monocytogenes and packaged with CL-AuNPs@CD-MOF/PLA-Zein exhibited a weight loss of only 6.20 ± 2.06%, compared to 17.74 ± 3.15% for PLA-Zein and 41.50 ± 3.01% for PE controls. Color stability was equally improved, with lightness values of 71.46 ± 1.47 retained under CL-AuNPs@CD-MOF/PLA-Zein packaging, versus 58.37 ± 0.86 for PLA-Zein and 23.34 ± 2.34 for PE. Mushrooms inoculated with E. coli and S. aureus followed consistent trends. These results establish CL-AuNPs@CD-MOF/PLA-Zein as a promising multifunctional antimicrobial packaging platform for sustainable food preservation. Full article

(1) Background: Bio-based plastics are an alternative for commonly used petroleum-based plastics, and their production will increase in the coming decades. In this work, two innovative bio-based plastics, i.e., polylactide-based (PLA-based) and polyhydroxybutyrate-based (PHBV-based), were studied with regard to their effect on the growth of higher plants (Sorghum saccharatum, Sinapsis alba) in the soil environment. (2) Methods: The experiments were conducted in pots filled with the Organisation for Economic Co-operation and Development (OECD) reference soil with or without one of the bioplastics at concentrations from 0.1% w/w to 12.5% w/w. This study is one of few works in which soil instead of another medium (e.g., deionised water) was used for the evaluation of the impact of microplastics on plant growth. (3) Results: Mustard (Sinapsis alba) was more sensitive to the presence of microplastics in the soil than sorghum (Sorghum saccharatum). The length of mustard shoots exposed to PLA-based plastic were shorter from 25% to about 56% than those in the control tests, while in the case of PHBV-based plastic, the decrease of mustard shoot length varied from 6% to 26%. The presence of the bioplastics studied, in particular the PLA-based one, at the levels of 2.5% w/w and higher contributed to reduced germination and shoot length and to the decrease in the relative chlorophyll content. (4) Conclusions: These three endpoints occurred to be more sensitive than the dry weight or elemental composition of plant biomass. They are recommended to be used in the evaluation of phytotoxicity of microbioplastics to study how to maintain the sustainability of the soil environment. Full article

Three-dimensional (3D) scanning and printing technologies enable the production of personalized rehabilitation splints, yet challenges such as scanning artifacts in complex anatomical areas (e.g., finger webs), lengthy post-processing, long printing times, and material limitations (e.g., brittleness and poor breathability) hinder routine clinical adoption. This feasibility study developed and evaluated a clinician-accessible protocol for fabricating cock-up wrist splints using 3D scanning (Creaform GO!SCAN 50 with VXelements 4.1), modeling (Materialise Magics), and fused deposition modeling printing with polylactic acid (PLA) on a MakerBot Replicator+. Five healthy participants wore the splints for one week, with user satisfaction assessed via the Quebec User Evaluation of Satisfaction with Assistive Technology (QUEST 2.0; average total score 4.14/5, range 3.75–4.42) questionnaire. An experienced occupational therapist provided expert feedback. High satisfaction was reported for weight (4.6/5) and ease of use (4.6/5), confirming advantages over traditional thermoplastic splints in lightness and esthetics. However, lower scores for durability (3.6/5), comfort (3.6/5), and effectiveness (3.6/5) stemmed from PLA brittleness (cracking under load or overtightening), rough surfaces despite vapor polishing, inadequate ventilation causing moisture buildup, and fit issues (e.g., pressure points). Printing time averaged 9–19 h per splint. The protocol demonstrates proof-of-concept feasibility for clinicians with basic computer techniques, but material constraints and process refinements are required for reliable application in patient populations. Full article

Instrumented mouthguards (iMGs) enable in vivo monitoring of head-impact exposure by reporting event-level peak linear acceleration (PLA) and peak angular acceleration (PAA) in contact sports. This case study describes head impacts in a world-class Muay Thai fighter during routine sparring sessions over a two-week period leading into a competitive bout. Seven sparring sessions were monitored using an iMG (PROTeQT, HitIQ), and only manufacturer (in-mouth)-flagged events above the device’s 8 g trigger threshold were analyzed. Event-level data were exported from the manufacturer portal; raw time-series signals and proprietary signal-processing parameters were not accessible, and no independent video verification was performed. Across the camp, 590 impacts were recorded. Mean PLA values were modest across sessions (7.6 to 19.5 g), with one event exceeding 106 g (max PLA 162.2 g). In contrast, PAA exhibited greater variability, with multiple device-flagged events exceeding 7900 rad/s², particularly in Sessions 4, 6, and 7, where maximum PAA values reached 19,862 to 26,850 rad/s². Overall, these data indicate that sparring was predominantly low in translational loading, while occasionally producing high recorded rotational peaks. Because outputs are device- and processing-pipeline-specific and were not independently verified, threshold-based severity banding and extreme peaks should be interpreted cautiously. This case demonstrates the potential utility of iMG monitoring to characterize session-to-session variability in sparring exposure and to inform practical sparring load management strategies aimed at reducing cumulative head-impact burden. Full article

The increasing demand for sustainable and environmentally friendly construction materials has spurred interest in biopolymer composites reinforced with agricultural waste. Rice husk (RH), a byproduct of rice milling, is abundant and rich in lignocellulosic fibers and silica, making it excellent for use in fiber-reinforced biopolymers. The novelty of this study lies in its integrated and construction-oriented evaluation of rice husk (RH)-reinforced biopolymers, combining mechanical, thermal, environmental, and economic perspectives within a single framework. The study introduces a novel comparative approach by benchmarking multiple polymer matrices-including PP, recycled HDPE, epoxy, PLA, and bio-binders-under unified quantitative performance criteria. Another key novelty is the identification of the dual functional role of silica-rich RH in simultaneously enhancing structural strength and flame retardancy while contributing to carbon emission reduction. With a high silica content (15–20%) and lignocellulosic structure, RH serves as a natural filler that enhances the performance of polymer matrices such as polypropylene (PP), epoxy, polylactic acid (PLA), and recycled polyethylene. Mechanically, RH-reinforced composites demonstrate significant improvements in tensile, flexural, and impact strength. For example, PP composites with NaOH-treated RH and coffee husks achieved tensile strengths between 27.4 MPa and 37.4 MPa, with corresponding Young’s modulus values ranging from 1656 MPa to 2247.8 MPa. Recycled HDPE-RH blends reached tensile strengths up to 74 MPa and flexural values of 39 MPa, validating their structural applicability. Epoxy matrices embedded with 0.45 wt.% RH nanofibers showed degradation thresholds of 411 °C and 678 °C, reflecting substantial thermal resistance. Flame retardancy is further improved by the presence of RH biochar, which leads to reduced peak heat release rate (PHRR) and enhanced char formation. In building insulation applications, RH-based composites exhibit low thermal conductivity values between 0.08 and 0.14 W/m·K, contributing to energy efficiency. Economically, RH reduces material costs by 30–40%, while environmentally, its integration lowers carbon emissions in PP composites by up to 10%, and promotes biodegradability. Despite challenges such as moisture absorption and interfacial adhesion, these can be mitigated through alkali treatment, compatibilizers (e.g., MAPP), or hybrid reinforcement strategies. Full article

Polylactide (PLA) has become widely adopted across biomedical, packaging, and manufacturing sectors due to its biodegradability and renewable sourcing. However, the rapid growth in PLA consumption has created urgent challenges related to waste management and the cleaning of processing equipment. This study investigates glycolysis as a promising chemical depolymerization pathway for PLA recycling and in situ reactor cleaning. A systematic analysis of four glycolysis agents (GA) (ethylene glycol, diethylene glycol, propylene glycol, and glycerol) was performed across molar PLA:GA ratios from 1:0.125 to 1:4 at 220 °C, targeting the efficient conversion of high-molecular-weight PLA (Mn ≈ 165 kDa) into low-molecular-weight oligomers. Gel permeation chromatography (GPC) demonstrated that propylene glycol exhibited the highest depolymerization efficiency, yielding oligomers with Mn as low as 200 g·mol⁻¹ even at minimal glycolysis agent ratios, while glycerol produced hydroxyl-rich oligomers optimal for subsequent lactide synthesis. Hydroxyl value (HV) measurements showed excellent agreement with theoretical values (<5% deviation), allowing us to make an assumption about an approximate, close to near-quantitative con-version. Glycolysis products with Mw below 400 g·mol⁻¹ displayed excellent water solubility, making them particularly attractive for reactor cleaning applications. Using glycerol-derived (GL) oligomers (PLA:GL = 1:0.25), purified L-lactide with a melting point of 98.1 °C and high purity (>99%) was obtained through thermocatalytic depolymerization and five recrystallization cycles, as confirmed by ¹H nuclear magnetic resonance (¹H NMR) and differential scanning calorimetry (DSC) analyses. The recovered lactide’s high purity renders it suitable for ring-opening polymerization, enabling closed-loop PLA recycling schemes. Overall, glycolysis emerges as a highly promising chemical recycling route complementary to hydrolysis and pyrolysis: propylene glycol maximizes depolymerization efficiency for cleaning applications, while glycerol optimizes oligomer functionality for lactide recovery and advanced material synthesis. Our results provide practical guidelines for selecting glycolysis agents and conditions for cleaning and recycling applications. Full article

Background: Low estrogen levels during menopause reduce nitric oxide (NO) production, contributing to decline in skeletal muscle quality and function. Although acute and short-term dietary nitrate supplementation has demonstrated promising effects, long-term benefits, particularly on muscle quality in postmenopausal women, are not well established. Objectives: The objective was to investigate the effects of long-term (12-week) nitrate-rich beetroot extract supplementation on morphological and functional muscle quality, rate of force development (RFD), maximal strength, and circulating nitrate/nitrite concentrations in postmenopausal women. Methods: In a randomized, double-blind, placebo-controlled design, 20 postmenopausal women (21 years ± 7 since menopause) consumed 20 g/day of a nitrate-rich beetroot extract (BET; 548 mg nitrate/day) or a nitrate-depleted beetroot extract (PLA; 43 mg nitrate/day) for 12 weeks. Outcome measures, including muscle quality (functional via muscle strength/thickness ratio; morphological via ultrasound echo intensity), RFD, maximal voluntary isometric contraction (MVIC), and serum nitrate/nitrite levels, were evaluated at baseline, 8 weeks, and 12 weeks. Results: BET significantly increased serum nitrate (0.005) and nitrite (0.022) levels compared to PLA at both week 8 and week 12. Morphological muscle quality also improved significantly in the BET group (interaction effect, p = 0.014). Early-phase rate of force development (RFD) increased between 30 and 100 ms, whereas late-phase RFD increased between 100 and 200 ms. RFD_peak also improved by week 8, and these gains were maintained through week 12 (interaction effect, p < 0.05). Although there was no significant difference between groups for functional muscle quality, MVIC increased at week 12 in the BET group, but no significant Time × Group interaction was observed. Conclusions: Twelve weeks of nitrate-rich beetroot extract supplementation improved morphological muscle quality and RFD, suggesting potential clinical relevance for preventing structural and neuromuscular function decline in postmenopausal women. This study was registered with ReBEC (RBR-87qh649) and approved on 8 October 2024. Full article

Primary glomerulonephritis encompasses a diverse group of kidney diseases with variable clinical trajectories and outcomes. Accurate prognostic stratification is critical for guiding individualized management and improving long-term renal survival. This narrative review synthesizes current evidence on the prognostic value of histological grading systems, circulating and urinary biomarkers, and integrative risk prediction models across major primary glomerulonephritis subtypes, including IgA nephropathy, membranous nephropathy, and focal segmental glomerulosclerosis. Emphasis is placed on the utility of established classification systems (e.g., Oxford, MEST-C, chronicity scores), emerging tissue and fluid biomarkers (e.g., PLA2R antibodies, complement components, cytokine profiles), and the validation of multivariable prognostic tools and nomograms. We highlight areas of convergence between histopathologic lesions and molecular markers, as well as the evolving role of machine learning in predictive modeling. Ultimately, combining morphological, biochemical, and algorithmic tools holds promise for precision risk assessment and treatment tailoring in primary glomerulonephritis. Full article

Post-harvest deterioration in strawberries is an urgent and critical issue that requires significant attention. Glycerol monolaurate (GML), a broad-spectrum food-grade antimicrobial agent, faces limited applicability due to its poor water solubility. In this study, a confined encapsulation strategy was employed to encapsulate GML within hydroxypropyl-γ-cyclodextrin (HPγCD), which improved the physicochemical properties of GML and enhanced its stability in the environment. The fiber morphology was observed through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), confirming the presence of a uniform, non-nodular core–shell structure. The Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) validated the successful encapsulation of GML within the cavity of HPγCD. Thermogravimetric analysis (TGA) demonstrated that the thermal stability of the core–shell system was significantly improved. In vitro release followed first-order kinetics (R² = 0.9842), with 79.5% of GML released over 68 h. The DPPH and ABTS assays demonstrated that PLA/GML-HPγCD NF exhibited sustained radical scavenging activity (p < 0.05, ANOVA). Compared to GML-HPγCD NF, PLA/GML-HPγCD NF exhibited prolonged antibacterial activity against Escherichia coli and superior antifungal efficacy in strawberry preservation. Meanwhile, PLA/GML-HPγCD NF significantly reduced lesion diameter and weight loss while maintaining hardness, total soluble solids, and vitamin C content over 8 days of storage. In conclusion, these characteristics highlighted the potential of P/G-HPγCD NF as a promising active packaging material for extending the shelf life of perishable fruits. Full article

Background: Sodium bicarbonate (SB) supplementation can enhance performance in short, high-intensity movements. However, its effectiveness in team sports such as rugby remains insufficiently explored. Methods: In this double-blind, parallel, controlled trial, 17 male professional rugby players ingested SB (0.3 g/kg) or a placebo 90 min before a high-intensity, rugby-specific training session monitored via GPS. The training session was conducted under real-world conditions to enhance ecological validity. Physical performance (countermovement jump, CMJ), fatigue markers (capillary lactate and ratings of perceived exertion, RPE), and gastrointestinal (GI) symptoms were assessed pre- and post-exercise. Results: No significant pre–post changes were observed in CMJ performance in either group. Lactate concentrations increased from pre- to post-exercise in both groups (both p < 0.001). The SB group showed higher GI symptom severity before, during and after exercise versus placebo, with several symptoms increasing over time solely in the SB group (p < 0.05). RPE increased similarly in both groups (SB: p = 0.012; PLA: p = 0.008). Due to the small sample size, only moderate-to-large within-group effects and very large between-group differences could be detected; therefore, the study was powered to detect moderate-to-large within-group effects but underpowered for detecting between-group differences. Conclusions: Acute SB ingestion at 0.3 g/kg did not result in detectable improvements in performance or fatigue markers during rugby-specific high-intensity training and was associated with a greater incidence of GI discomfort; however, the study was underpowered to detect small between-group differences. This study was registered on 23 May 2025 on ClinicalTrials.gov (NCT07017582). Full article