Search Results (16,724)

Modern agriculture faces the critical need to develop sustainable, safe, and effective strategies for enhancing productivity, protecting plants and animals, and ensuring food security. Challenges posed by antibiotic resistance and the adverse environmental and consumer health impacts of chemical agents are driving the search for eco-friendly alternatives. In this context, bacteriocins—naturally occurring antimicrobial peptides synthesized by diverse bacteria—represent a promising alternative to traditional chemical compounds. This article reviews the potential and current advances in bacteriocin applications across agricultural sectors, with particular focus on their targeted antagonistic activity, structural diversity, commercial bacteriocin-based products, and their utilization in livestock farming, crop production, poultry farming, and aquaculture. Key findings demonstrate that bacteriocins, particularly nisin and pediocin PA-1, exhibit potent activity against major agricultural pathogens including Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, and Escherichia coli, with efficacy rates reaching 90% in mastitis treatment and significantly reducing pathogen loads in poultry and aquaculture systems. Commercial products such as Nisaplin, Wipe Out, and ALTA 2431 have been successfully implemented in veterinary medicine and food production. In aquaculture, bacteriocins effectively control Lactococcus garvieae, Aeromonas spp., Vibrio spp., and Pseudomonas aeruginosa, contributing to sustainable disease management with minimal environmental impact. It can be suggested that bacteriocins may play an essential role in combating pathogens and offer viable alternatives to conventional antibiotics across primary food production systems, though optimization of production methods and regulatory frameworks remains essential for broader commercial adoption. Full article

This study developed a design framework for porous mixtures using a 100% sustainable non-bituminous epoxy–polyurethane binder system. Conventional design protocols for porous asphalt mixtures exhibit limitations in accurately controlling void content and mixture composition. This study proposed a novel design framework for porous mixtures containing 100% sustainable binder based on statistical analysis and theoretical calculations. The relationships among target air voids, binder content, and aggregate gradation were systematically analyzed, and calculation formulas for coarse aggregate, fine aggregate, and mineral filler contents were derived. A mix design framework was further established by applying the void-filling theory, where the combined volume of binder, fine aggregate, and filler equals the void volume of the coarse aggregate skeleton, thereby ensuring precise control of the target void ratio. Additionally, mixing procedures were investigated with emphasis on feeding sequence, compaction method, and mixing temperature. Results indicated that the optimized feeding sequence significantly improved binder distribution; specimens compacted using the Marshall double-sided compaction method achieved a density of 89.60%. Rheological analysis revealed that at 30 °C, the viscosities of sustainable binder and polyurethane filler were 1280 mPa·s and 6825 mPa·s, respectively, suggesting optimal mixture uniformity. The proposed methodology and process parameters provide essential technical guidance for engineering applications of porous mixtures containing 100% sustainable binder. Full article

Compared to reinforcing concrete with steel bars, rebars—made of fiber-reinforced plastic—have a high potential for resource savings in the construction industry due to their corrosion resistance. For the large-volume market of reinforcement elements, efficient manufacturing processes must be developed to ensure the best possible bond behavior between concrete and rebar. In contrast to established FRP-rebars made with thermosetting materials, the use of a thermoplastic matrix enables surface profiling without severing the edge fibers as well as subsequent bending of the bar. The rebars to be produced in this study are based on the process of reactive thermoplastic pultrusion of continuously glass fiber reinforced aPA6. Their surface must enable a mechanical interlocking between the reinforcement bar and concrete. Concepts for a profiling device have been methodically developed and evaluated. The resulting concept of a double wheel embossing unit with a variable infeed and an infrared preheating section is built as a prototype, implemented in a pultrusion line, and further optimized. For a comprehensive understanding of the embossing process, reinforcement bars are manufactured, characterized, and evaluated under parameter variation according to a statistical experimental plan. The present study demonstrates the relationship between the infeed, preheating temperature, and haul-off speed with respect to the embossing depth, which is equivalent to the rib height. No degradation of the Young’s modulus was observed as a result of the profiling process. Full article

Children and adolescents with disabilities experience disproportionate challenges in achieving recommended levels of physical activity (PA) and adequate sleep, two core determinants of health and functional well-being. This systematic review examined associations between meeting PA guidelines and sleep duration among youth with disabilities. Following PRISMA guidelines, MEDLINE, PsycARTICLES, and SPORTDiscus were searched through Spring 2024 for studies assessing PA and sleep in children and adolescents (<18 years) with disabilities using subjective or objective measures. Data were extracted from 28 studies (N = 138,016) and synthesized using qualitative methods and regression-based quantitative analyses to examine the effects of diagnosis category and PA guideline adherence on sleep duration. The diagnosis type was associated with sleep duration, with youth with autism spectrum disorder (ASD) exhibiting shorter sleep than those with physical disabilities. Meeting PA guidelines (≥60 min/day) was associated with longer sleep duration among youth with ASD, but not consistently across other diagnostic groups. Qualitative findings further indicated diagnosis-specific variability, with PA positively associated with sleep outcomes in ASD, attention deficit/hyperactivity disorder, and epilepsy, and mixed associations observed for cerebral palsy and intellectual disability. These findings suggest that PA may support sleep health in specific disability groups. Given persistently low PA participation among youth with disabilities, integrating accessible, diagnosis-specific PA opportunities within school, community, and clinical settings may represent a feasible strategy to improve sleep and overall health. Full article

Background/Objectives: Sarcopenia (Sp) and obesity (Ob) have significant negative effects on steatotic liver disease (SLD). Here, we examined the effects of sarcopenic Ob (SO) on liver fibrosis in patients with SLD. Methods: We included 811 patients who visited our outpatient clinic and underwent FibroScan (Echosens, France). Liver stiffness (LS) was assessed using body mass index (BMI) and grip strength (GS). We conducted a similar analysis by converting the difference in estimated glomerular filtration rate (dGFR) based on creatinine and cystatin C levels into GS. Results: The cutoff values for distinguishing metabolic dysfunction-associated steatotic liver disease (MASLD; 298 patients) with LS > 10 kPa (advanced fibrosis) were set separately for men and women using receiver operating characteristic analysis. BMI was set at >26 kg/m² in women and >27 kg/m² in men (modified Ob (mOb)), and GS was set at <16 kg in women and <31 kg in men (modified Sp (mSp)). The ratio of advanced fibrosis was higher in the group with both mSp and mOb (mSpOb) than in the group with mSp alone or mOb alone in MASLD or alcoholic liver disease (ALD, 97 patients). However, this association has not yet been observed in other diseases. The dGFR was used to set the cutoff value corresponding to advanced fibrosis. Sp-dGFR (SpdG) was >1.14 in women and >−0.76 in men in the MASLD group. mSpOb, SpdG and Ob are associated with advanced fibrosis in MASLD logistic regression analysis. Conclusions: SO, assessed using BMI and GS or dGFR, was associated with elevated LS in patients with SLD. Full article

Type 1 Diabetes (T1D) is thought to result from the interaction of genetic and environmental factors, with different studies highlighting a potential role for the gut microbiota and its metabolites in modulating immune responses and disease development. We hypothesized that patients with T1D exhibited altered levels of circulating bacterial metabolites compared with healthy controls (HC), and that these metabolite profiles were associated with key demographic, clinical, and analytical features of the disease. A total of 91 T1D patients and 58 HC were recruited. Plasma samples were collected and analyzed with gas chromatography coupled to mass spectrometry for the detection of the metabolites: short-chain fatty acids (SCFAs: acetate [AA], propionate [PA], isobutyrate [IBA], butyrate [BA], isovalerate [IVA], valerate [VA], and methyl valerate [MVA]), medium-chain fatty acids (MCFAs: hexanoate [HxA] and heptanoate [HpA]) and para-cresol (p-cresol). We also calculated the ratios between the different SCFAs with AA. T1D patients showed significantly higher circulating AA levels than HC, along with reduced PA/AA and IBA/AA ratios, indicating an altered SCFA profile. SCFA diversity was lower in T1D patients, with reduced detection of BA, and total SCFA levels were increased mainly due to elevated AA. AA levels were higher and SCFA ratios lower in women with T1D compared with healthy women, while p-cresol levels were higher in men with T1D than in healthy men. In T1D patients, AA levels positively correlated with HbA1c, whereas PA/AA, IBA/AA, and BA/AA ratios showed negative correlations, particularly in women. MV/AA and non-AA/AA ratios were inversely associated with glucose levels, again, mainly in women. p-cresol levels correlated positively with age and ferritin and were higher in T1D patients with liver dysfunction or hypertension. Therefore, we can conclude that T1D is associated with a marked alteration in circulating gut-derived metabolites, characterized by increased AA levels, particularly in women, and an imbalance in SCFA ratios that correlates with glycemic control. These findings, together with the associations observed for p-cresol and metabolic comorbidities, support a role for the gut microbiota–host metabolic axis in T1D. Full article

Background: Multimodal large language models (MLLMs) integrating multiple AI systems and unimodal large language models (LLMs) represent distinct approaches to clinical decision support. Their comparative performance against human clinical experts in complex cardiovascular emergencies remains inadequately characterized. Objective: To compare the performance of a combined MLLM system (GPT-4V + Med-PaLM 2 + BioGPT), a unimodal LLM (ChatGPT-5.2), and human physicians from multiple centers (radiologists, emergency medicine specialists, cardiovascular surgeons) on aortic dissection clinical questions across diagnosis, treatment, and complication management domains. Methods: This multicenter cross-sectional study was conducted across five tertiary care centers in Turkey (Elazığ, Ankara, Antalya). A total of 25 validated multiple-choice questions were categorized into three domains: diagnosis (n = 8), treatment (n = 9), and complication management (n = 8). Questions were administered to the MLLM, ChatGPT-5.2 (Unimodal), and nine physicians from five centers: radiologists (n = 3), emergency medicine specialists (n = 3), and cardiovascular surgeons (n = 3). Statistical comparisons utilized chi-square tests. Results: Overall accuracy was 92.0% for the MLLM and 96.0% for ChatGPT-5.2 (Unimodal). Among human physicians, cardiovascular surgeons achieved 96.0%, radiologists 92.0%, and emergency medicine specialists 89.3%. The MLLM excelled in diagnosis (100%) but showed lower performance in treatment (88.9%) and complication management (87.5%). No significant differences were observed between AI models and human physician groups (all p > 0.05). Conclusions: Both the MLLM and unimodal ChatGPT-5.2 demonstrated performance within the range of human clinical experts in this controlled assessment of aortic dissection scenarios, though definitive conclusions regarding equivalence require larger-scale validation. These findings support further investigation of complementary roles for different AI architectures in clinical decision support. Full article

Background/Objectives: The effects of online physical activity (PA) interventions on executive function (EF) and Attention-Deficit Hyperactivity Disorder (ADHD) symptoms are promising; nonetheless, their benefits for Latinx youth remain unclear. Methods: This study explores levels of adherence, cognitive and behavioral outcomes and acceptability of an online PA intervention, Zing Performance, among a Latinx youth sample; only a few of the participants completed their condition (n = 6). Results: There was wide variability in adherence levels at mid-treatment (n = 5) and high-level adherence at post-treatment (n = 2). A Mann–Whitney test yielded a statistically significant (p = 0.004) improvement in the treatment group’s inattention symptoms at mid-treatment (n = 5), compared to the Waitlist Control; (WLC; n = 6). EF and hyperactivity/impulsivity were not significantly different. Further, pre-, mid- and post-participant trajectory data revealed that one participant benefited significantly from treatment, one participant demonstrated little to no response to treatment, and most of the WLC participants remained in the severity ranges throughout the 12 weeks. The parents of the two children who completed treatment reported high levels of acceptability informally and on the quantitative measure. Conclusions: Exploratory findings support further investigation of Zing among Latinx families with cultural consideration to study procedures. The lessons learned from this study are valuable for future research procedures and interventions with this marginalized population. Full article

Background: Persistent liver pathology despite a sustained virologic response (SVR) to hepatitis C virus (HCV) therapy is a major clinical concern. This is particularly relevant for people with HIV (PWH) with HCV coinfection, a population prone to accelerated liver disease progression. This study aimed to characterize the plasma miRNA profile in PWH with cirrhosis one year after successful completion of HCV therapy, and to explore their relationship with metabolite alterations. Methods: This cross-sectional study enrolled 47 PWH who achieved HCV clearance with antiviral therapy. Using plasma samples collected approximately one year after completion of HCV therapy, participants were stratified into two groups based on liver stiffness measurement (LSM): compensated cirrhosis (n = 32, LSM ≥ 12.5 kPa) and non-cirrhosis (n = 15, LSM < 12.5 kPa). Plasma miRNAs and metabolites were determined using small RNA sequencing and untargeted capillary electrophoresis-mass spectrometry (CE-MS), respectively. Significantly differentially expressed (SDE) miRNAs were identified using generalized linear models (GLM) with a negative binomial distribution, and their correlation with metabolite levels was quantified using Spearman’s correlation. Results: In the cirrhosis group (n = 32), we identified a distinct signature of 15 SDE miRNAs (9 upregulated, 6 downregulated) compared to the non-cirrhotic group (n = 15), showing hsa-miR-10401-3p, hsa-miR-548ak, hsa-miR-141-3p, and hsa-miR-3940-3p the largest expression changes. miRNA-gene interaction and pathway enrichment analysis suggested that these 15 SDE miRNAs potentially regulate multiple genes involved in immune response and amino acid metabolism. In addition, correlation analyses with our metabolomic data revealed significant associations between specific SDE miRNAs and amino acids and their derivatives. Specifically, the expression of upregulated miRNAs (e.g., hsa-miR-10401-3p and hsa-miR-16-5p) was positively correlated with plasma levels of L-methionine and its derivatives, while downregulated miRNAs (e.g., hsa-miR-625-5p) were inversely correlated with L-tryptophan. Conclusions: In cirrhotic PWH with history of HCV coinfection, a distinct plasma miRNA signature linked to dysregulated amino acid metabolism is found one year after completion of HCV therapy. This underscores that the HCV cure does not equate to complete hepatic recovery, highlighting the critical need for long-term monitoring in this high-risk population. Full article

Wine lees, a byproduct of winemaking, represent an underutilized source of β-glucans with potential functional applications in food. This study aimed to extract β-glucans using two methods—acid–alkaline treatment and autolysis assisted by ultrasound—and evaluate their effects when incorporated into low-fat stirred yogurt. The extracted β-glucans were added at a concentration of 0.3% (w/w), and rheological measurements were conducted over 20 days of storage. All yogurt samples showed shear-thinning behavior, with apparent viscosity decreasing from 10⁵ mPa·s at low shear rates (0.1 s⁻¹) to 10³ mPa·s at 100 s⁻¹. Yogurt with β-glucans from autolysis retained higher viscosity and viscoelastic moduli (G′ and G″), indicating better structural integrity. Time-dependent tests showed up to 45% decrease in shear stress over 10 min of continuous shearing in the sample with chemically extracted β-glucans, compared to only 28% for autolysis-derived ones. Oscillatory tests confirmed that all samples behaved as weak gels (G′ > G″). These findings suggest that β-glucans obtained via autolysis can improve the textural stability of yogurt, offering potential for functional dairy development and valorization of wine industry byproducts. Full article

Protected areas (PAs) constitute a fundamental strategy for mitigating biodiversity loss. The land–sparing approach has expanded in response to international agreements, but expansion of PAs does not guarantee conservation objectives. The objective was to assess PA effectiveness in conserving Nothofagus antarctica forests in Santa Cruz (Argentina), evaluating human impacts associated with fires, animal uses, and harvesting. The research was conducted within pure native forests in Santa Cruz, Argentina. This province encompasses 52 protected areas, representing the highest concentration of conservation units within the forested landscapes across Argentina. At least eight PAs included N. antarctica forests. Three land tenure categories were evaluated: protected areas (PAs), a buffer of 15 km from PA boundaries on private lands (BL), and private lands (PL) outside the buffer. In total, 103 stands were sampled, where 38 variables were assessed (impacts, soil, forest structure, understory, and animal use). Three indices were developed to analyze ecosystem integrity: forest structure (FI), soil (SI), and animal use (AI). PAs presented the highest FI (0.64 for PA, 0.44 for BL, and 0.30 for PL) and AI (0.60 for PA, 0.55 for BL, and 0.52 for PL), and together with buffer areas, the highest SI (0.43 for PA, 0.47 for BL, and 0.32 for PL). PAs were clearly distinct from private lands; however, sustained actions for livestock exclusion, harvest regulation, and fire management remain necessary for future sustainable planning at the landscape level. Full article

Background/Objectives: Patients with eosinophilic chronic obstructive pulmonary disease (COPD) often remain symptomatic despite optimized triple inhaled therapy. Dupilumab is a fully human monoclonal antibody that blocks the IL-4 receptor alpha subunit, thereby inhibiting IL-4 and IL-13 signaling. Evidence from randomized trials supports dupilumab for add-on treatment of type 2-high COPD, but data referring to short-term effectiveness in clinical practice are quite limited. Methods: We conducted an observational, compassionate-use study enrolling 13 consecutive outpatients with eosinophilic COPD (blood eosinophils ≥ 300 cells/µL) receiving add-on biologic therapy with dupilumab 300 mg every two weeks. Clinical (CAT, mMRC), functional (spirometry and body plethysmography), and inflammatory parameters (blood eosinophils/basophils, fibrinogen, FeNO) were evaluated at baseline and after four weeks of treatment. Safety was monitored after injection in a clinical setting, as well as via weekly phone follow-up. Results: Participants (84.6% male; mean age 67.08 ± 11.42 years) experienced rapid and clinically meaningful improvements at four weeks. CAT score decreased from baseline 21.40 ± 6.22 to 14.00 ± 5.58 (p < 0.001) and mMRC scale from 2.90 ± 0.73 to 1.80 ± 0.63 (p < 0.0001), respectively. Pre-bronchodilator FEV₁ increased from baseline 1.35 ± 0.65 L to 1.59 ± 0.84 L (p < 0.05), and FVC from 2.36 ± 0.92 L to 2.83 ± 1.11 L (p < 0.01). A marked lung deflation was observed: indeed, residual volume declined from baseline 4.17 ± 1.98 L to 3.47 ± 2.07 L (p < 0.05), with a concomitant reduction in specific effective airway resistance (from baseline 3.15 ± 1.77 to 2.43 ± 1.44 kPa·s; p < 0.05) associated with significant increases in mid-expiratory flow (FEF₂₅₋₇₅: from baseline 0.62 ± 0.38 to 0.86 ± 0.71 L/s; p < 0.05) and peak expiratory flow (3.80 ± 1.40 to 4.48 ± 1.79 L/s; p < 0.01). Type 2 inflammatory biomarkers changed as follows: blood eosinophil count fell from baseline 390.0 ± 43.75 to 190.0 ± 65.47 cells/µL (p < 0.001); blood basophil number decreased from baseline 37.50 ± 13.89 to 26.25 ± 13.02 cells/µL (p < 0.001); plasma fibrinogen lowered from baseline 388.4 ± 54.81 to 334.9 ± 72.36 mg/dL (p < 0.01); FeNO levels dropped from baseline 23.95 ± 18.10 to 14.00 ± 2.04 ppb (p < 0.0001). Dupilumab was well tolerated, and no treatment-related serious adverse events or discontinuations were detected. Conclusions: Within an exploratory context of daily medical activity referring to eosinophilic COPD already treated with maximal inhaled therapy, we found relevant therapeutic effects of a four-week add-on treatment with dupilumab. In particular, our patients manifested rapid improvements in symptoms, airflow limitation, and lung hyperinflation, paralleled by significant decrements of type 2 inflammatory signatures. Such encouraging results were associated with a favorable short-term safety profile. However, larger and longer studies are necessary to corroborate these preliminary findings. Full article

Gravity waves (GWs) are an important dynamic process in the planetary atmosphere. They are typically excited by convection, topography, or other sources from the lower atmosphere and propagate upwards. The GWs have a significant effect on the global atmospheric circulation on Mars. However, the lack of high-resolution data from previous observations has resulted in an insufficient understanding of GWs in the Martian atmosphere, particularly in terms of its global distribution and long-term evolution characteristics at different altitudes. Based on multiple years of Mars Climate Sounder (MCS) limb observations on board the Mars Reconnaissance Orbiter (MRO), we conducted a detailed study of the global distribution, seasonal and interannual variations in Martian atmospheric GWs with vertical wavelengths ranging from 9 to 15 km at three different altitude ranges, i.e., the low-altitude range of 200–20 Pa (Lp, ~10–30 km), the mid-altitude range of 20–2 Pa (Mp, ~30–50 km), and the high-altitude range of 2–0.2 Pa (Hp, ~50–70 km). The results indicate complex regional and north–south differences, as well as night–day variations, in the spatial distribution of GWs. Particularly, a three-wave structure of the GW activity is observed over mountainous regions in the mid-to-low latitudes of the Northern Hemisphere. The peak longitude range of this structure closely matches the mountainous terrain. In addition, our results reveal the presence of bands of GW aggregations in the mid- to-high latitudes of the Northern Hemisphere in the Mp and Hp layers, which may be caused by the instability of the polar jet. There are also obvious seasonal and interannual variations in GW activities, which are related to topography, polar jets, and large dust storms. The interannual variations in GWs imply that, in addition to the well-known large seasonal dust storms, complex interannual variations in atmospheric activity over the polar jets and in the complex topography at mid-to-low latitudes on Mars may also exist, which deserve further studies in the future. Full article

Microalgae photobioreactors (PBRs) are promising building-integrated biotechnologies for carbon capture and biomass production; however, their high energy requirements for artificial lighting remain a significant energy barrier in cold climates. This study developed an integrated spectral–optical energy modeling framework to evaluate two PBR deployment strategies in State College, PA: rooftop daylight-exposed integration and basement installation with solar-assisted lighting. Results show that fiber-optic daylighting can supply a substantial fraction of photosynthetically useful light without introducing additional internal heat loads, while photovoltaics sized at approximately 0.40–0.55 kWDC per reactor can offset the annual PBR lighting energy use when sufficient roof area is available. Whole-building energy simulations further reveal that rooftop PBR integration reduces total annual space energy consumption by ~21% relative to basement placement due to lower artificial lighting and cooling loads. When combined, PV and fiber systems can fully meet basement PBR lighting demand, whereas rooftop configurations may rely more on grid electricity. Economically, fiber-optic daylighting achieves comparable lighting offsets at roughly half the annualized cost of PV-based systems, subject to surface-area and routing constraints. Overall, solar-assisted lighting strategies markedly improve the operational sustainability of building-integrated PBRs in cold climates, with fiber-optic daylighting offering substantial spectral and thermal advantages, subject to surface-area availability and routing-related design constraints. Full article

Background and Objectives: Acute respiratory failure (ARF) has a heterogeneous course in the emergency department (ED), and early prediction of noninvasive mechanical ventilation (NIMV) failure is difficult. The PaCO₂–ETCO₂ gap reflects ventilation–perfusion mismatch and increased physiologic dead space; however, the prognostic value of its short-term change during NIMV is unclear. This study evaluated baseline, post-treatment, and delta (post–pre) PaCO₂–ETCO₂ gap values for predicting intubation, intensive care unit (ICU) admission, and mortality in ED patients with ARF receiving NIMV. Materials and Methods: This prospective observational study enrolled adults (≥18 years) treated with NIMV in a tertiary ED. Exclusion criteria included GCS < 15, intoxication, pneumothorax, trauma, pregnancy, gastrointestinal bleeding, need for immediate intubation/CPR, or incomplete data. ETCO₂ was recorded within the first 3 min of NIMV and at 30 min; concurrent arterial blood gases provided PaCO₂. The PaCO₂–ETCO₂ gap was calculated at both time points and as delta. Outcomes were intubation, ICU admission, and mortality. ROC analyses determined discriminatory performance and cutoffs using the Youden index. Results: Thirty-four patients were included (50% female; mean age 73.26 ± 10.07 years). Intubation occurred in 9 (26.5%), ICU admission in 20 (58.8%), and mortality in 10 (29.4%). The post-treatment gap and delta gap were significantly higher in intubated patients (p = 0.007 and p = 0.001). For predicting intubation, post-treatment gap > 10.90 mmHg yielded AUC 0.807 (p = 0.007; sensitivity 77.8%, specificity 76.0), while delta gap > 2.90 mmHg yielded AUC 0.982 (p = 0.001; sensitivity 88.9%, specificity 92.0). Delta gap also predicted ICU admission (cutoff > 0.65 mmHg; AUC 0.746, p = 0.016) and mortality (cutoff > 2.90 mmHg; AUC 0.865, p = 0.001). Conclusions: In ED ARF patients receiving NIMV, an increasing PaCO₂–ETCO₂ gap—especially the delta gap—was associated with higher risks of intubation, ICU admission, and mortality, supporting serial CO₂ gap monitoring as a practical early warning marker of deterioration. Full article