Search Results (16)

The study focused on the distribution of polyphenolic compounds, iridoids, organic acids, and sugars, as well as in vitro antioxidant, anti-inflammatory, antidiabetic, antiproliferation, and antibacterial potential, and physicochemical properties between the skin and flesh of 10 haskap berry (Lonicera caerulea var. kamtschatica Sevast.) cultivars. It was found that the content of individual bioactive compounds significantly depended on the fruit cultivar and the analyzed morphological part. Anthocyanins, kaempferol derivatives and iridoids dominated in the skin, which significantly correlated with most of the analyzed health-promoting properties. In turn, the flesh showed a higher content of quercetin derivatives, sugars and organic acids. No differences were found in the content of phenolic acids, flavan-3-ols and antibacterial activity. The most beneficial properties were shown for the cultivar (cv.) ‘Honeybee’. The study suggests that haskap fruit skin is a valuable raw material for use in the pharmaceutical and food industries. Full article

Lonicera caerulea is a species known for its fruit with a rich health-promoting composition and the high frost resistance of its bushes. The increase in the popularity of this species and the number and area of plantations increases the risk of diseases and pests. However, the use of ozone gas may involve the risk of physiological damage to the plant. In this experiment, in 2022–2023, the physiological response of six varieties of Lonicera caerulea L. to gaseous ozone at a concentration of 5 ppm·1 min, 5 ppm·3 min and 5 ppm·5 min was determined. The flavonoid–nitrogen index (NFI) remained unchanged at 0.33 in both non-ozonated leaves and those exposed to a 5 ppm·3 min dose of ozone. In general, ozonation did not lead to significant changes in the physiological parameters observed for most of the varieties studied. The mean performance index (P_itotal) value of the ozonated leaves decreased by 23.1% for LE ‘Lori’ and 23.8% for ‘139-24’, after applying an ozone dose of 5 ppm·5 min in 2022. A significant decrease of 34.3% in the average transpiration rate (E) was observed after the use of 5 ppm·5 min ozone in plants of the variety ‘21-17’ across both years of cultivation. The different effects of the ozone doses used may indicate different reactions depending on the variety used and the year of cultivation. Overall, the study found that ozone does not have a phytotoxic effect on most varieties, which may indicate different reactions and differences between varieties. The use of an appropriate dose of ozone did not cause any disruption in the selected physiological parameters of Lonicera caerulea L. plants. The absence of phytotoxicity in some varieties may allow the use of ozonation treatments in agriculture; however, further research on the long-term effects on plants is required. Full article

The aim of this study was to use a rapid and non-invasive spectrofluorimetric method to evaluate the qualitative changes occurring in stored Kamchatka berry preserves. Honeysuckle berries were preserved by freezing (−24 °C) and pasteurisation with and without sugar addition. Pasteurised samples were stored at 6 ± 1 °C and 22 ± 1 °C for 9 months. During storage, spectrofluorimetric spectra in the bioactive compounds’ fluorescence range were registered. The obtained synchronous spectra were used in a statistical analysis involving principal component analysis (PCA) and linear discriminant analysis (LDA). The analysis of both types of registered spectra indicated that sugar addition could stabilise some phenolic compounds, like gallic acid, p-coumaric acid, and phloridzin. Moreover, some differences in the degradation rate of each analysed compound were observed depending on the preservation method used. Besides the phenolic compounds, other fluorescent compounds like B-vitamins and chlorophyll forms were also observed. Pasteurisation caused the distinct degradation of protochlorophyll forms, whereas practically no changes in the amounts of vitamins B₃ and B₉ were observed. Based on the results of statistical analyses of PCA and LDA, the effect on the products’ composition was moderate for the storage time and relatively low in the case of the storage temperature. The obtained results indicated that spectrofluorimetry would be a useful method for the detailed characterisation of fruit products. Full article

The aim of this study was to investigate the qualitative and quantitative fruit profiles of ten cultivars (cvs.) of haskap berry (Lonicera caerulea var. kamtschatica Sevast.) to determine their antioxidant activity (ABTS test, CUPRAC test, ability to capture superoxide (O₂˙⁻) and hydroxyl radicals (OH˙)), cytotoxic activity (against cancer cell lines breast, MCF-7; colon, HT-29; and melanoma, SK-Mel-28) and physicochemical properties. Most of the selected cultivars had not previously been analyzed for these properties. A total of 19 polyphenolic compounds were identified in the fruits of the tested genotypes, with a quantitative range of 2166.3–3597.0 µg/g. The polyphenol profile was dominated by anthocyanins (90.0–92.4%), and the remaining classes occurred in the following order: phenolic acids > flavonols > flavan-3-ols. The highest concentrations of these polyphenol groups were found in the cultivars ‘Honeybee’, ‘Sinij Uties’ and ‘Usłada’. The fruits of these cultivars were also characterized by the highest antioxidant activity (546.6–683.5 µg/mL for O₂˙⁻ and 541.2–652.1 µg/mL for OH˙) and cytotoxic activity (103.6–649.2 µg/mL). The data obtained indicate that the fruits of the new haskap cultivars are a good source of bioactive compounds with possible health-promoting properties. Full article

Lonicera caerulea L. fruits are a rich source of vitamins, organic acids, and phenolic compounds, which are characterised by their health-promoting properties. The content of bioactive compounds in this fruit may vary depending on the cultivar and the harvest date. This study analysed the effect of applying 5 ppm gaseous ozone for 1, 3, and 5 min on the chemical properties of L. kamtschatica varieties and newly created clones of L. emphyllocalyx for three years of cultivation. The fruits harvested from L. emphyllocalyx, depending on the year of harvest, had significantly larger size and weight compared to L. kamtschatica. On average, the acidity of the L. emphyllocalyx clones was 6% higher than other tested varieties. The average content of ascorbic acid was highest in L. emphyllocalyx clone ‘21-17’—57.80 mg·100 g⁻¹; the year of harvest will significantly affect the content of vitamin C, reaching the highest level in 2022—53.92 mg·100 g⁻¹. The total content of polyphenols was significantly dependent on the year of cultivation; reaching, on average, 54.8% more in 2022 compared to the rest of the years. The total antioxidant value using the FRAP, DPPH, and ABTS methods varied depending on the variety; exposure to ozone significantly increased the antioxidant value in each case. On the basis of the study, both botanical varieties can be used in food processing. Gaseous ozone exposure can significantly influence chemical composition, increasing the health-promoting value of fruit. Full article

Kamchatka berry (Lonicera caerulea var. kamtschatica) and haskap (Lonicera caerulea var. emphyllocalyx) fruit are important sources of bioactive compounds, mainly polyphenols, but also macro- and microelements. Physico-chemical analysis showed that wheat beers with added fruit were characterised by an average 14.06% higher ethanol content, lower bitterness and intense colour compared to the control, which was a wheat beer without added fruit. Wheat beers enriched with kamchatka berry fruit, including the “Aurora” variety, had the highest polyphenolic profile (e.g., chlorogenic acid content averaged 7.30 mg/L), and the antioxidant activity of fruit-enriched wheat beers determined by the DPPH method showed higher antioxidant activity of wheat beers enriched with kamchatka berry fruit, while those determined by the FRAP and ABTS methods showed higher antioxidant activity of wheat beers enriched with haskap fruit, including the “Willa” variety. Sensory evaluation of the beer product showed that wheat beers enriched with kamchatka berry fruits of the “Duet” variety and haskap fruits of the “Willa” variety were characterised by the most balanced taste and aroma. On the basis of the conducted research, it follows that both kamchatka berry fruits of the “Duet” and “Aurora” varieties and haskap fruit of the “Willa” variety can be used successfully in the production of fruity wheat beers. Full article

Blue honeysuckle (Lonicera caerulea var. kamtschatica Sevast.) is a valuable berry crop with a unique biochemical composition. It is rich in vitamins, minerals, and biologically active substances. Different species and cultivars of honeysuckle require different cultivation conditions in the field of accelerated reproduction in vitro. Taking into account the high clonal replication potential of the plant, we conducted research on the chelated-iron form’s influence on the micropropagation productivity of the blue honeysuckle “Lulia” cultivar at the multiplication, rooting, and subsequent acclimatization stages of microplants. In a preliminary study, five types of iron chelates were tested with carboxyl- and phosphorus-containing ligands: Fe(III)-EDTA, Fe(III)-DTPA, Fe(III)-EDDHA, Fe(III)-HEDP, and Fe(II)-HEDP. Each type of iron chelate was applied at four concentrations: standard, decreased by 2 times, and increased by 1.5 times and 2 times in the basic Murashige and Skoog (MS) nutrient medium. It was found that the blue honeysuckle “Lulia” had a selectivity to the type of iron chelate that was used. The nutrient-medium modifications with iron chelates, which caused the plant response, contributed to a significant improvement in the plant’s physiological status and increased its survival rate during the microplant’s acclimation to the nonsterile conditions stage. At the rooting stage, an increase in the rooting rate of up to 100% (Fe (III)-EDDHA), an increase in the number of shoots by 1.5–2 times, and an increase in the number of roots by 1.4–1.9 times were observed. The positive effect of the iron’s chelated forms was also observed at the acclimation stage. According to the results of the research, the most suitable iron forms for clonal micropropagation of blue honeysuckle were carboxyl-containing Fe(III)-EDDHA and phosphonate-containing Fe(II)-HEDP. Moreover, the effectiveness of both complexonates was confirmed in a wide concentration range: Fe(III)-EDDHA from (×1.0) to (×2.0), and Fe(II)-HEDP from (×0.5) up to (×1.5). Full article

Lonicera caerulea fruits are a rich source of vitamins, organic acids, and phenolic compounds, which are characterised by their health-promoting properties. The content of bioactive compounds in this fruit may vary depending on the cultivar and the harvest date. The fruits of the L. caerulea var. kamtschatica cultivars ‘Duet’ and ‘Aurora’ and the L. caerulea var. emphyllocalyx cultivars ‘Lori’, ‘Colin’ and ‘Willa’ were used in this study. L. emphyllocalyx fruit, especially the cultivar ‘Willa’, was characterised as having a higher acidity by an average of 29.96% compared to L. kamtschatica. The average ascorbic acid content of the L. kamtschatica fruit was 53.5 mg·100 g⁻¹ f.w., while L. emphyllocalyx fruit had an average content that was 14.14% lower. The antioxidant activity (determined by DPPH, FRAP, and ABTS) varied according to the cultivar and the species of fruit analysed. The total polyphenol content differed significantly depending on the cultivar analysed; fruits of the L. emphyllocalyx cultivar ‘Willa’ were characterised by the lowest content of total polyphenols—416.94 mg GAE·100 g⁻¹ f.w.—while the highest content of total polyphenols—747.85 GAE·100 g⁻¹ f.w.—was found in the fruits of the L. emphyllocalyx cultivar ‘Lori’. Lonicera caerulea fruits contained 26 different phenolic compounds in their compositions, of which the highest content was characterised by cyanidin 3-O-glucoside (average: 347.37 mg·100 g⁻¹). On the basis of this study, it appears that both L. kamtschatica fruits and L. emphyllocalyx fruits, especially of the cultivars ‘Lori’ and ‘Willa’, can be used in food processing. Full article

Honeysuckles are frost tolerant plants providing early-ripening fruits with health-promoting properties which have been used in traditional medicine in China. This study evaluates the impact of the climatic conditions of two areas on the chemical composition and antioxidant activity (AOA; by DPPH—2,2-diphenyl-1-picrylhydrazyl and photochemiluminescence assays) of eight cultivars of honeysuckle berries (Lonicera caerulea L. var. kamtschatica Pojark) of various ripening times. Expectedly, chemical composition and AOA values varied depending on the cultivars, locality and selected methods. Berries from Lednice (the area with more sunshine) showed higher average contents of total monomeric anthocyanins (TMAC; pH differential absorbance method), vitamins C and E and total phenolics (high-performance liquid chromatography). In contrast, berries from Žabčice (the area with more rain) performed higher average contents of total phenolics and flavonoids (UV/VIS spectroscopic analyses). Interestingly, fundamental amounts of chlorogenic acid were determined irrespective of the locality. Regarding TMAC and vitamin C content, early ripening Amphora from both areas has been assessed as the best cultivar; concerning the content of phenolic compounds, Fialka from both areas and Amphora from Lednice is considered as the most valuable. The obtained results may facilitate the selection of the most valuable cultivars for both producers and consumers. Full article

Oxidation is one of the most prevalent factors responsible for meat product deterioration. Due to their potential health risks, commonly used synthetic antioxidants are beginning to be frowned upon by customers. The industry is searching for a natural replacement. In our study, we incorporated blackcurrant (Ribes nigrum L.) and Kamchatka honeysuckle (Lonicera caerulea var. Kamtschatica) extracts into raw-cooked meat products (frankfurters) as natural antioxidants. We observed that both extracts at concentrations of 3 mL·kg⁻¹ were able to significantly (α = 0.05) postpone lipid oxidation in our samples, with results comparable to vitamin C (0.5 mg·kg⁻¹) addition. Moreover, we did not observe negative effects of the extracts on the product’s color, pH, or textural properties. Negative results were reported in the sensory evaluation of honeysuckle addition samples. This could have been caused by the natural strong and bitter taste of honeysuckle, which was transferred to the extracts and, subsequently, into the meat product. Full article

The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L.kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L. kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average). Full article

Total polyphenols content (TPC), antioxidant activity (AA) and polyphenolic spectrum assay of three underutilized fruit species—black crowberry (Empetrum nigrum), honeyberry (Lonicera kamtschatica) and European cranberry (Vaccinium oxycoccos) is the aim of the present work. TPC and AA assay was performed by spectrophotometry and “individual phenolic compounds” were established by high-performance liquid chromatography. The results showed that TPC ranged from 1.61 ± 0.16 (European cranberry) up to 5.65 ± 0.01 (Black cowberry) gallic acid mg/g fresh weight. The highest value of DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay was determined in black crowberry fruit (7.43 ± 0.34 mmol TROLOX/g fresh weight of fruit). In all samples, the most prevalent phenolic acid was ferulic acid with the highest content in the samples of black crowberry (77.73 ± 3.99 µg/g FW of fruit), black crowberry and honeyberry were present by the highest level of gallic acid (21.82 ± 1.53; 15.07 ± 2.45 µg/g FW of fruit). Honeyberry represented a valuable source of quercetin (12.18 ± 7.88 µg/g FW of fruit), whereas European cranberry and honeyberry are a source of rutin (28.48 ± 0.83 and 27.99 ± 1.78 µg/g FW of fruit, respectively). The results of statistical analyses proved significant differences between cowberry and European cranberry in TPC content and in AA among assayed species. In the same way, statistically significant differences were confirmed in phenolic acids among the assayed species except for European cranberry-honeyberry (in trans p-coumaric, protocatequic and cinnamic acid) and honeyberry-cowberry (in cinnamic acid content). The catechin content is significantly influenced by species (p ≤ 0.05), on the other hand the species has no influence on the resveratrol content (p > 0.05). Each of the studied species can be considered as valuable source of total polyphenols and individual phenolic compounds. Polyphenolic compounds were most frequently accumulated in the vacuole as well as in the outer layers of pericarp. Full article

Lonicera caerulea L. (haskap) berries are widely known for their richness in anthocyanins. In this study, such fruits were assessed for their nutritional and chemical composition, but also as sources of anthocyanins with great colouring properties to be applied in foodstuff. Haskap presented high levels of water, four free sugars (mainly fructose and glucose), five organic acids (mainly citric, malic, and quinic), α- and γ-tocopherol, twenty fatty acids (with prevalence of linoleic acid), and eight phenolic compounds, among which six were anthocyanins (mainly cyanidin-3-O-glucoside). The extract presented great antioxidant properties, evaluated through TBARS and OxHLIA assays, as well as antimicrobial capacity against six bacteria and six fungi. Two colourants were obtained by spray-drying haskap juice with maltodextrin and a mixture of maltodextrin and arabic gum. These formulations were stable over 12 weeks of storage at room and refrigerated temperature, without significant variations in colour parameters and in anthocyanins concentration. They were considered safe for consumption once neither microbial contamination nor cytotoxicity in non-tumour cells were detected. The results obtained allow for the consideration of haskap as a promising source of colourants to be applied not only in the food industry, but also in other fields that rely on artificial colourants. Full article

Iridoid and polyphenol profiles of 30 different honeysuckle berry cultivars and genotypes were studied. Compounds were identified by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC-ESI-qTOF-MS/MS) in positive and negative ion modes and quantified by HPLC-PDA. The 50 identified compounds included 15 iridoids, 6 anthocyanins, 9 flavonols, 2 flavanonols (dihydroflavonols), 5 flavones, 6 flavan-3-ols, and 7 phenolic acids. 8-epi-Loganic acid, pentosyl-loganic acid, taxifolin 7-O-dihexoside, and taxifolin 7-O-hexoside were identified in honeysuckle berries for the first time. Iridoids and anthocyanins were the major groups of bioactive compounds of honeysuckle constituents. The total content of quantified iridoids and anthocyanins was between 128.42 mg/100 g fresh weight (fw) (‘Dlinnoplodnaya’) and 372 mg/100 g fw (‘Kuvshinovidnaya’) and between 150.04 mg/100 g fw (‘Karina’) and 653.95 mg/100 g fw (‘Amur’), respectively. Among iridoids, loganic acid was the dominant compound, and it represented between 22% and 73% of the total amount of quantified iridoids in honeysuckle berry. A very strong correlation was observed between the antioxidant potential and the quantity of anthocyanins. High content of iridoids in honeysuckle berries can complement antioxidant properties of phenolic compounds. Full article

Iridoid profiles of honeysuckle berry were studied. Compounds were identified by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry UPLC-ESI-qTOF-MS/MS in positive and negative ions mode. The MS fragmentation pathways of detected iridoid glycosides were also studied in both modes. In the negative ESI mass spectra, iridoids with a methyl ester or lactone structure have preferentially produced adduct [M + HCOOH − H]⁻ ions. However, protonated ions of molecular fragments, which were released by glycosidic bond cleavage and following fragmentation of aglycone rings, were more usable for iridoid structure analysis. In addition, the neutral losses of H₂O, CO, CO₂, CH₃OH, acetylene, ethenone and cyclopropynone have provided data confirming the presence of functional substituents in the aglycone. Among the 13 iridoids, 11 were identified in honeysuckle berries for the first time: pentosides of loganic acid (two isomers), pentosides of loganin (three isomers), pentosyl sweroside, and additionally 7-epi-loganic acid, 7-epi-loganin, sweroside, secologanin, and secoxyloganin. The five pentoside derivatives of loganic acid and loganin have not been previously detected in the analyzed species. Honeysuckle berries are a source of iridoids with different structures, compounds that are rarely present in fruits. Full article