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Keywords = Lebrunia neglecta

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23 pages, 5111 KB  
Article
Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones Anthopleura dowii and Lebrunia neglecta
by Santos Ramírez-Carreto, Beatriz Miranda-Zaragoza, Nuno Simões, Ricardo González-Muñoz and Claudia Rodríguez-Almazán
Mar. Drugs 2024, 22(1), 12; https://doi.org/10.3390/md22010012 - 23 Dec 2023
Cited by 6 | Viewed by 4548
Abstract
The bioprospecting of sea anemone tissues and secretions has revealed that they are natural libraries of polypeptides with diverse biological activities that can be utilized to develop of biotechnological tools with potential medical and industrial applications. This study conducted a proteomic analysis of [...] Read more.
The bioprospecting of sea anemone tissues and secretions has revealed that they are natural libraries of polypeptides with diverse biological activities that can be utilized to develop of biotechnological tools with potential medical and industrial applications. This study conducted a proteomic analysis of crude venom extracts from Anthopleura dowii Verrill, 1869, and Lebrunia neglecta Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides, of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes, oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors, among others, were identified. Interaction analysis and functional annotation indicated that these proteins are primarily involved in endoplasmic reticulum metabolic processes such as carbon metabolism and protein processing. In addition, several proteins related to oxidative stress were identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase. Our results provide novel information on the polypeptide composition of the crude venom extract from sea anemones, which can be utilized to develop molecules for therapeutic tools and industrial applications. Full article
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12 pages, 2624 KB  
Article
A Sea Anemone Lebrunia neglecta Venom Fraction Decreases Boar Sperm Cells Capacitation: Possible Involvement of HVA Calcium Channels
by Fernando Lazcano-Pérez, Karina Bermeo, Héctor Castro, Zayil Salazar Campos, Isabel Arenas, Ariana Zavala-Moreno, Sheila Narayán Chávez-Villela, Irma Jiménez, Roberto Arreguín-Espinosa, Reyna Fierro, Humberto González-Márquez, David E. Garcia and Judith Sánchez-Rodríguez
Toxins 2022, 14(4), 261; https://doi.org/10.3390/toxins14040261 - 7 Apr 2022
Cited by 7 | Viewed by 3574
Abstract
Sea anemones produce venoms characterized by a complex mixture of low molecular weight compounds, proteins and peptides acting on voltage-gated ion channels. Mammal sperm cells, like neurons, are characterized by their ion channels. Calcium channels seem to be implicated in pivotal roles such [...] Read more.
Sea anemones produce venoms characterized by a complex mixture of low molecular weight compounds, proteins and peptides acting on voltage-gated ion channels. Mammal sperm cells, like neurons, are characterized by their ion channels. Calcium channels seem to be implicated in pivotal roles such as motility and capacitation. In this study, we evaluated the effect of a low molecular weight fraction from the venom of the sea anemone Lebrunia neglecta on boar sperm cells and in HVA calcium channels from rat chromaffin cells. Spermatozoa viability seemed unaffected by the fraction whereas motility and sperm capacitation were notoriously impaired. The sea anemone fraction inhibited the HVA calcium current with partial recovery and no changes in chromaffin cells’ current kinetics and current–voltage relationship. These findings might be relevant to the pharmacological characterization of cnidarian venoms and toxins on voltage-gated calcium channels. Full article
(This article belongs to the Special Issue Cnidarian Venom)
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