Search Results (8)

True slime molds (Eumycetozoa) remain under-explored globally, particularly in water-logged forest habitats. Despite evidence suggesting a high biodiversity potential in the Knyszyn Forest of north-eastern Poland, no systematic effort had previously been undertaken there. In the present survey, plant substrates from eight swampy sub-compartments were incubated for over four months, resulting in the detection of fifteen slime mold species. Four of these taxa are newly reported for northern and north-eastern Poland, while several have been recorded only a handful of times in the global literature. These findings underscore how damp, nutrient-rich conditions foster Eumycetozoa and demonstrate the effectiveness of moist-chamber culturing in revealing rare or overlooked taxa. Current evidence shows that, although slime molds may occasionally colonize living plant or fungal tissues, their influence on crop productivity and tree vitality is negligible; they are therefore better regarded as biodiversity indicators than as pathogens or pests. By establishing a replicable framework for studying water-logged environments worldwide, this work highlights the ecological importance of swamp forests in sustaining microbial and slime mold diversity. Full article

The Danube Clouded Yellow (Colias myrmidone) has experienced one of the most dramatic declines among European butterflies. To estimate genetic diversity in the last population in Poland that has survived in the Knyszyn Forest (KF), we analyzed mitochondrial (COI) and nuclear (EF-1α) polymorphisms in individuals sampled in 2014 and 2022. The results were compared with genetic data obtained in 2014 from a recently extirpated nearby population (Czerwony Bór, CB). Because mtDNA polymorphisms in insects can be modulated by endosymbionts, the samples were screened for Wolbachia. The polymorphism of EF-1α indicated that diversity was gradually decreasing. The KF experienced rapid demographic processes, manifested by a significant change in allele frequency. The small differentiation in nuclear markers between the KF and CB in 2014 suggests that the regional population used to be genetically uniform. Four COI haplotypes that were identified in this study probably belong to two different haplogroups. Wolbachia was detected only in individuals with one specific haplotype, and the prevalence was female-biased, suggesting the induction of two reproductive manipulations. The most common COI haplotype found in Poland was the same as that reported from other parts of Europe, not only for C. myrmidone but also C. caucasica. These results allow us to question the distinctiveness of each taxa. Full article

Microorganisms are vital in leaf litter decomposition and contribute significantly to global nutrient cycling. However, there is a need for improved understanding of the taxonomic and functional diversity of litter-associated bacteria. The Knyszyn Forest comprises a unique ecosystem providing diverse microhabitats for microorganisms in central Europe, similar to the southwestern taiga in many respects. This study presents the results of high-throughput sequencing performed for Betula pendula, B. pubescens, and Carpinus betulus litter-associated microbial communities from northern Poland. Microbial assemblage composition and structure at different stages of litter decomposition revealed the domination of phyllosphere-associated taxa of Sphingomonas and Pseudomonas in bacterial communities in the early stages. Meanwhile, at the later stages of decomposition, the representation of soil-associated bacterial communities, such as Pedobacter, was higher. This study identifies key bacteria (Pedobacter, Mucilaginibacter, and Luteibacter) as pivotal in nutrient cycling through cellulose and hemicellulose decomposition, dominating later decomposition phases. Taxonomic analysis based on functional markers associated with nitrogen metabolism highlights the pivotal role of specific Pseudomonadota (Proteobacteria) taxa in driving nitrogen cycling dynamics during litter decomposition. Most of these taxa were unclassified at the genus level, particularly in the later stages of litter decomposition, and are crucial in mediating nitrogen transformation processes, underscoring their significance in ecosystem nutrient cycling. Full article

Peat and lacustrine sediments are a valuable source of knowledge about past biodiversity. Plant macrofossil remains were identified in sediments of mires in northeastern Poland’s Knyszyn Forest. Among them, the remains of species currently absent in this area, such as Potamogeton pusillus, P. friesii, P. filiformis, Myriophyllum alterniflorum, and Cladium mariscus, have been found. In addition, the history of Betula humilis and its possible correlations with another species of bush birch—Betula nana—were of interest. Radiocarbon dating allowed the presence of the studied species to be placed within a time frame, and it was thus established that aquatic species existed in the area under study during the Late Glacial and the turn of the Holocene. Cladium mariscus occurred during the Greenlandian and Meghalayan stages of the Holocene and then became intolerant of habitat changes that occurred. The coexistence of two species of birch known to exist since the Late Glacial was interrupted in the Northgrippian. B. nana, then disappeared from the area, and B. humilis continued to occur as it was more resistant to the changing environmental conditions. It must be emphasized, however, that these conclusions were made possible by the researchers’ access to undisturbed deposits. The mires present in the area of study remain in good condition, providing important and relevant materials for palaeoecological research. Full article

Springs are often considered as “hotspots” of aquatic biodiversity. However, small organisms, such as rotifers, substantially contribute to secondary production, but they are rarely investigated in springs. We surveyed the rotifer fauna of 47 springs in the Knyszyn Forest (north-eastern Poland) in summer and autumn 2014–2015. We hypothesized that stream communities of Rotifera may be specific to this habitat. Rotifer samples were taken using a Plexiglas tube in the spring current. Concurrently, water temperature, pH, conductivity, water redox potential and phosphorus and nitrogen concentrations were determined. The springs did not differ statistically in water quality and were stable regarding their temperature and conductivity. Rotifer densities and mean number of monogonont species were very low both in summer and autumn. Nevertheless, the total number of species recorded in all springs was relatively high and accounted for 101 in total. Although strongly differentiated, the rotifer fauna contained a set of several species common to most of the springs. Most of the recorded species are eurytopic and widely spread in water ecosystems. Only the concentration of magnesium and chloride ions had an impact on Monogononta numbers. Full article

The local populations of relict plant taxa living near the limits of their geographical range are particularly vulnerable to extinction. For example, Salix lapponum is one of the rarest and most endangered glacial relicts in Western and Central Europe. In Poland, the number of its sites has dramatically decreased over the past few decades, prompting us to take conservation measures focused on saving endangered populations. During a field experiment aimed at the reconstruction of the downy willow population in the Knyszyn Forest (NE Poland), 730 individuals of the species were planted in four different natural sites. The seedlings were obtained by micropropagation from parts of vegetative individuals taken from the most abundant population of this species in eastern Poland (Lake Bikcze). The success of the reintroduction, measured by the number of individuals that survived 2–3 years in the wild and took up growth, was about 67%, however, with low flowering efficiency (7.5%). Additionally, monitoring showed significant differences in plant survival and growth rates under different habitat conditions prevailing at the site and with different cover from competing species, especially tall grasses. However, the restoration projects for relict shrub species should include periodic removal of competing plants and protection of plants from trampling and browsing by herbivorous mammals to increase reintroduction success. Full article

The Puszcza Knyszyńska springs are an important element of the environment, conditioning the high geo- and biodiversity in the region. These springs are layered outflows that drain the waters of the Quaternary level. More than 200 outflows have been cataloged, and 80 of them have measured/estimated yields and analyses of their water qualities. During periods of low water levels, the discharge efficiency most often fluctuated within a range of 0.5–2.0 dm³·s^–1. In light of the applicable legal standards, the tested waters were of excellent quality. They belonged to the two hydrochemical types HCO₃-Ca and HCO₃-Ca-Mg, indicating the Quaternary aquifer’s homogeneity in the post-glacial areas. Small concentrations of most of the chemical parameters of water were found in the forest springs. Larger transformations in the chemical composition of water occurred in the outflows located in agricultural land and rural areas. A characteristic feature of the chemical composition of the water in the region’s lowland springs was an increased concentration of biogenic compounds (nitrogen, phosphorus, and carbon compounds), both organic and inorganic. The average concentration of dissolved organic carbon in the water of the lowland springs in summer exceeded 4 mg·dm⁻³. In some outflows, most often of a swamp nature or located in peat areas, the concentration of dissolved organic carbon even exceeded a dozen or so mg·dm⁻³. Full article

Radioactivity measurements of 61 therapeutic peat mud samples from the Podsokoldy deposits, near Suprasl, were performed using gamma spectrometry. The authors identified the presence of 13 isotopes with the arithmetic mean of activity (in Bq kg⁻¹): ¹³⁷Cs-7, ⁴⁰K-24, ²⁰⁸Tl-1, ²¹²Bi-3, ²¹²Pb-2, ²²⁸Ac-2, ²¹⁰Pb-33, ²¹⁴Bi-11, ²¹⁴Pb-11, ²²⁶Ra-53, ²³⁴Th–47. The effective dose obtained during treatment with 15 peat mud baths (lasting 30 min) was 0.078 μSv. Use of peat mud compresses in the same number and period of exposure to the entire body surface caused absorption of a dose of 0.153 μSv. The authors discuss the probability of tissue radiation from isotopes present in the peat mud. In light of radiobiological knowledge, the therapeutic effect of ionizing radiation during peat mud therapy appears to be very unlikely. Full article