Search Results (428)

An integrated approach combining metabolomics, network pharmacology, and molecular docking was employed to systematically explore the serum-absorbed components of jujube, their potential targets, and regulatory pathways. UPLC-MS/MS was used to characterize the absorbed components, while network pharmacology was applied to predict potential targets associated with alcoholic liver disease (ALD). A total of 10 absorbed components and 323 common targets were identified. Among the key components, quercetin, (-)-epigallocatechin, and methyl gallate exhibited strong binding affinities to eight core targets, including AKT serine/threonine kinase 1 (AKT1) and mitogen-activated protein kinase 1 (MAPK1), with quercetin showing the highest content. Jujube intervention significantly altered the serum metabolic profiles of healthy rats, with distinct differences observed between the control and jujube-treated groups. Bioinformatics analysis revealed that the differential metabolites were mainly enriched in the diterpenoid biosynthesis pathway. These findings provide a systematic and preliminary characterization of the serum-absorbed components of jujube, their potential ALD-related targets, and their regulatory effects on serum metabolism in healthy rats. This study provides a preliminary theoretical reference and direction for further research on the potential role of jujube in ALD. Full article

Rising consumer demand for functional beverages has accelerated the development of health-promoting, fruit-based ready-to-drink (RTD) products. This study investigated the effects of incubation temperature (50–80 °C) and time (60–240 min) on pectinase-assisted extraction (0.1% v/v) of date palm (Phoenix dactylifera L., Bahi variety) juice and subsequently formulated antioxidant-rich RTD beverages by blending the optimized extract with quince and jujube juices. The optimal extraction condition (50 °C, 60 min) was selected based on maximizing bioactive compound recovery rather than yield, achieving total phenolic content of 326.33 mg GAE/100 mL, total carotenoid content of 1.08 mg β-carotene equivalents/100 mL, and strong antioxidant activity (DPPH: 514.06; FRAP: 595.38 µmol TE/100 mL). Although maximum yield (81.25%) was obtained at 60 °C for 240 min, functional quality was prioritized. Six RTD formulations were developed using a constrained simplex-lattice mixture design. All blends exhibited significantly enhanced phenolic content, carotenoids, and antioxidant capacity compared to the control, while pH and acidity remained stable (p > 0.05). Sensory evaluation indicated that the formulation containing 70% date palm, 15% quince, and 15% jujube achieved the highest acceptability (6.50). These findings highlight the potential of this tri-fruit blend as a functional RTD beverage, warranting further studies on shelf-life stability. Full article

To analyze the phytogenic components of honey of Amorpha fruticosa L. (AFH) and establish a targeted quantitative method, the liquid chromatography-mass spectrometry (LC-MS) based metabolomic technology was used in this study. Firstly, high performance liquid chromatography—quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) untargeted metabolomics technology was used to screen candidate markers by comparing AFH metabolites with plant chemicals of Amorpha fruticosa L. Afterward, high performance liquid chromatography—triple quadrupole tandem mass spectrometry (HPLC-QQQ-MS/MS) was used to verify and identify the candidate markers, confirming ononin as the characteristic phytogenic marker of AFH, and determining its content range in AFH as 76.84–93.27 μg/kg (absent in acacia, rape, jujube, and Galla chinensis honey). Then, network pharmacology and molecular docking techniques were adopted to explore the gastric protective mechanism of ononin, and the results showed that ononin strongly binds AKT1 (binding free energy −8.0677 kcal/mol). Using the established method, the LC-MS analytical method for ononin in honey established in this study may be used for the authenticity identification of the characteristic phytogenic markers of AFH. Full article

The health benefits of jujubes are closely related to their active substances. In this study, the active substances in ‘huizao’ (HZ), ‘junzao’ (JZ), ‘hamidazao’ (HMDZ), ‘kashigerexiaozao’ (KSGEXZ) and ‘yuanlingzao’ (YLZ) jujube varieties were identified via ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). After identification, a total of 1583 metabolites were found in jujube fruits, covering 13 major categories including alkaloids, amino acids and their derivatives. Pairwise comparisons revealed 518–755 differentially abundant metabolites across cultivars, and each variety had its main dominant metabolites. Through network pharmacology, 141 potential disease-related bioactive compounds were screened out, and key compounds were identified through molecular docking. These substances bind to the key target TP53 through hydrogen bonding and hydrophobic interactions. Among them, catechin, naringenin, and coniferin had the strongest binding affinities. These data increase our understanding of the active components of jujube and provide valuable information for the sustainable cultivation of jujubes for health applications. Full article

Jujube (Ziziphus jujuba Mill.) is a functional food with both edible and medicinal properties. It is rich in various bioactive compounds and holds significant development value and application prospects in food nutrition, medicine, and health. This review systematically summarizes the research progress on the synthesis mechanism and pharmacological activities of phenolic compounds in jujube fruits, clarifies the composition of their main components, sorts out the research advances in extraction technologies of jujube phenolic compounds, and focuses on analyzing the content differences and distribution patterns across cultivars and tissue parts. On this basis, it examines the regulatory mechanisms of phenolic compound synthesis in depth, with a particular focus on elucidating the regulatory networks of genes and transcription factors involved in flavonoid biosynthesis. Meanwhile, this review comprehensively summarizes the pharmacological activities of phenolic compounds in jujube fruits, including antioxidant, anticancer, antibacterial, anti-inflammatory, and hypoglycemic effects. It also elucidates the molecular mechanisms underlying these bioactivities, such as regulating signaling pathways and scavenging free radicals. Finally, it analyzes the limitations of current research and proposes key directions for future development. This review provides theoretical support and a scientific basis for the in-depth development and utilization of jujube phenolic compounds as well as for the research and development of related functional foods and drugs. Full article

Jujube wine production faces the technical challenge of excessive methanol formation due to the high pectin content of the raw material. This study systematically compared five pre-fermentation treatments (pectinase, chitosan, bentonite, gelatin, and diatomite) for their effects on the methanol content and overall quality of jujube wine, aiming to identify an adsorbent pretreatment strategy that effectively reduces methanol levels while preserving wine quality. Using pectinase treatment as a reference control (representing conventional industrial pectin degradation), the results showed that all adsorbent treatments reduced the pectin content in jujube juice, thereby influencing methanol generation in the fermented wine. Notably, chitosan pretreatment exhibited the most pronounced methanol reduction (113.35 mg/L), which was 59.20% lower than that of the pectinase reference group (277.65 mg/L), and remained far below the methanol limits stipulated by Chinese, EU, and OIV standards (≤400 mg/L). This effectiveness is attributed to the positively charged nature of chitosan, which efficiently removes negatively charged pectin through electrostatic adsorption, thereby blocking methanol formation at the source. Chitosan treatment also resulted in the highest alcohol content (5.82%) and significantly reduced organic acid levels but concurrently led to a slight decrease in some key aroma esters. However, sensory evaluation revealed that jujube wine produced from the chitosan-pretreated juice maintained a harmonious overall taste profile, with an overall score comparable to that of the pectinase reference group. In comparison, bentonite and gelatin showed moderate effectiveness, while diatomite performed poorly. Notably, although pectinase treatment yielded the lowest pectin content, it paradoxically resulted in the highest methanol levels, highlighting the critical mechanism of pectin degradation (rather than removal) promoting methanol formation. In conclusion, chitosan was recommended as an effective pre-fermentation treatment strategy for producing jujube wine with a significantly reduced methanol risk. This study provided a theoretical basis and technical reference for the safe, high-quality industrial production of jujube wine. Full article

Background: Wild jujube (Ziziphus jujuba var. spinosa) exhibits remarkable tolerance to saline-alkali stress, yet its molecular mechanisms remain poorly understood. 3-ketoacyl-CoA synthase (KCS) is a key enzyme involved in the biosynthesis of very-long-chain fatty acids (VLCFAs), which constitute pivotal precursors for membrane lipids involved in stress adaptation. Methods: Through genome-wide analysis and molecular biology techniques, 20 ZjKCS genes were identified. Results: The ZjKCS genes were grouped into nine subfamilies, exhibiting highly conserved gene structures, motifs, and functional domains within each subfamily. Two pairs of collinear gene pairs were identified, with the ZjKCS12-ZjKCS18 pair retaining core conserved functions despite intense purifying selection. ZjKCS genes are rich in cis-acting elements associated with light transduction, phytohormone responses, and abiotic stress adaptation. Tissue-specific expression patterns of ZjKCS under light, ABA (abscisic acid), and MeJA (methyl jasmonate) treatments were analyzed by quantitative real-time PCR (qRT-PCR). Under saline-alkali stress, ZjKCS genes were significantly upregulated, with most showing strong sustained induction during later treatment stages. Conclusions: These findings indicate that the ZjKCS family participates in saline-alkali stress and abiotic stress adaptation, potentially by enhancing VLCFA synthesis to reinforce and remodel membrane lipid structure. This study provides a foundation for elucidating lipid-mediated stress resistance mechanisms in stress-tolerant fruit trees. Full article

Jujube (Ziziphus jujuba Mill.) cultivation in arid regions of China faces severe soil constraints, including high alkalinity, low organic matter content, and poor water retention. Although soil amendments have demonstrated potential for improving soil quality, their combined effects on soil–plant–microbe interactions in desert agroecosystems remain poorly understood. This study conducted a three-year field experiment in a desert jujube orchard in southern Xinjiang, China, to evaluate six nitrogen fertilizer management strategies: urea alone (CK) or combined with biochar (NB), bentonite (NP), graphene (NS), biochar plus bentonite (NBP), or microbial inoculants (NW). Soil physicochemical properties, enzyme activities, bacterial community structure, and jujube yield were analyzed. Structural equation modeling (SEM) was employed to elucidate the pathways linking soil amendments to crop productivity. Results showed that NBP was the most effective in improving soil physical structure, significantly reducing bulk density and enhancing water retention capacity compared to the control. The NBP treatment also enhanced soil organic matter (30% increase), available phosphorus (119% increase), and urease activity (44% increase), resulting in the highest jujube yield (7.14 kg per tree). Bacterial community analysis revealed that NBP significantly increased Shannon diversity and enriched Actinobacteriota and Proteobacteria. SEM analysis indicated that urease activity served as a significant mechanistic pathway linking soil organic matter improvements to enhanced crop productivity. These findings demonstrate that combined application of biochar and bentonite with nitrogen fertilizer represents an effective strategy for improving soil quality, enhancing microbial functionality, and increasing crop yield in desert jujube orchards, providing a practical and synergistic amendment combination for sustainable soil management and productivity enhancement in arid agroecosystems. Full article

Ziziphus jujuba Mill. is a characteristic resource with both medicinal and edible values. At present, its lactic acid bacteria-fermented products are plagued by ambiguous variety selection and low added value. To clarify the variety-specific regulatory effects of Z. jujuba cultivars on metabolic profiles during lactic acid bacteria fermentation, this study analyzed the metabolic characteristics of fermented broths of Tan jujube, Jun jujube, and Ban jujube under a unified fermentation system using LC-MS untargeted metabolomics technology. Significantly differential metabolites were screened with the criteria of p < 0.05 and VIP > 1, and the metabolic regulatory mechanisms were further elucidated, combined with KEGG pathway enrichment analysis. The results showed that a total of 570 metabolites were identified in the three fermented broths. Tan jujube was enriched in linolenic acid, Ban jujube was rich in D-xylitol and dethiobiotin, and Jun jujube had prominent contents of S-adenosylmethionine and pyridoxine. All the aforementioned metabolites are involved in important physiological processes such as anti-inflammation and intestinal homeostasis maintenance. The differential metabolites were mainly enriched in 6 key pathways, including central carbon metabolism, ABC transporters, and phenylpropanoid biosynthesis, among which central carbon metabolism and ABC transporters were the core regulatory pathways. This study constructed an association network of Z. jujuba variety–differential metabolite–key pathway, systematically elucidated the metabolic differentiation mechanisms of fermented broths from different Z. jujuba cultivars, and provided a scientific basis for the precise selection of Z. jujuba varieties dedicated to fermentation and the targeted development of high-value-added functional fermented foods. Full article

Drought stress is a significant environmental factor affecting plant growth, fruit quality and distribution. Wild jujube is an important species of eco-economic forest tree. In this study, two wild jujube families, ‘NO. 1’ (tolerant) and ‘NO. 5’ (sensitive), which show significant differences in morphological and physiological indicators in drought treatment, are considered. Compared with the ‘NO. 5’, the ‘NO. 1’ exhibited lower water loss, leaf yellowing and abscission rates, as well as reduced malondialdehyde (MDA) content, while showing higher superoxide dismutase (SOD) activity and elevated levels of soluble sugars (SS), soluble proteins (SP), and proline (Pro). In contrast, the ‘NO. 5’ suffered more severe damage to leaf epidermal cells compared with the ‘NO. 1’, accompanied by a significant decline in net photosynthetic rate (A) and instantaneous water use efficiency (WUEi). Transcriptomic profiles between two wild jujube families with markedly different drought responses (withholding water for 15 days) are shown. The two wild jujube families included 3238 up-regulated and 2675 down-regulated differentially expressed genes (DEGs). Many DEGs enriched in the GO and KEGG pathways are related to antioxidant activity, transmembrane transport, carbohydrate biosynthesis and metabolism, plant hormones, and photosynthesis. The biosynthesis of amino acids, the MAPK signaling pathway, plant hormone signal transduction, and flavonoid and alkaloid biosynthesis were the transcriptome modifications most significantly altered by drought stress. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to verify the precision of the RNA-seq data. ZjJIP23-1, ZjbZIP53, ZjSPS8, ZjCAO, ZjADH1 and ZjERF39 may play important roles in the drought tolerance of the wild jujube. This study provides a solid foundation for further studies on the complex mechanisms and breeding of drought-resistant plants in wild jujube. Full article

Under conditions of limited irrigation and excessive fertilizer application in the arid regions of Xinjiang, it is essential to adopt well-coordinated strategies to improve yield and crop water productivity (WP). In this study, a comparative experiment was conducted with three irrigation levels, T1 (100% crop evapotranspiration, ET_c), T2 (75% ET_c), and T3 (50% ET_c), combined with three potassium application rates, K1 (540 kg ha⁻¹), K2 (360 kg ha⁻¹), and K3 (180 kg ha⁻¹). The objective was to investigate their effects on the yield, quality, and economic benefits of jujube trees. Limited irrigation amounts significantly affected the photosynthetic characteristics, growth parameters, and ET_c of jujube trees, whereas potassium fertilizer levels primarily regulated fruit development and yield formation. Compared with full irrigation, mild deficit irrigation caused a moderate yield reduction but significantly enhanced economic returns due to the improved water productivity and fruit quality. In contrast, severe water deficit led to substantial decreases in growth parameters and economic benefits by 12.87–45.70% and 81.69%, respectively. Potassium application demonstrated a significant threshold effect, with the K2 treatment showing greater improvements in fruit quality indices, including reducing sugars, vitamin C, and other key quality parameters, compared to the K3 treatment. Based on hierarchical–grey relational analysis, the combination of 75% ET_c and 300 kg K ha⁻¹ was identified as the optimal water–potassium management strategy. The net profit was 29,199 CNY. The benefit–cost ratio increased to 3.63, and the WP improved by 16.17% compared to full irrigation. Thus, this study provides an important theoretical basis and technical support for water-saving and quality-improving cultivation of jujube trees in arid regions. Full article

The jujube (Ziziphus jujuba Mill.) is a significant economic fruit tree, valued for its nutritional and medicinal properties. However, advances in functional genomics are hindered by the lack of an efficient transformation system. To overcome the limitations of conventional explant, we established a fruit-pulp-derived, callus-based Agrobacterium-mediated transformation system using fruit-pulp harvested 50 days after pollination. Through orthogonal experimental design, 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid were identified as key regulators for inducing high-quality, friable callus in two jujube genotypes, ‘JZ60’ and ‘LWCZ’. This system revealed significant genotype-specific variation in auxin requirements for callus proliferation and in differential antibiotic sensitivity. Transformation efficiency, as evaluated by fluorescence screening, was primarily determined by acetosyringone concentration and the binary vector architecture. The results revealed that the compact pCY (kanamycin resistance) vector achieved higher transformation efficiency (up to 77.8%) than pCAMBIA1301, whereas the pCAMBIA1301 (hygromycin resistance) vector enabled more uniform transgene expression. Integration and expression of the ZjCBF3 transgene were confirmed by polymerase chain reaction (PCR), reverse transcription quantitative PCR, and green fluorescent protein fluorescence assays. This study established a fruit-pulp-based callus transformation system for jujube, providing a rapid platform for its functional genomic studies. Full article

Water resources in arid oases are extremely scarce, and the quality of irrigation water and groundwater depth are key factors affecting soil secondary salinization and maintaining high and stable crop yields. This study focuses on the oasis irrigation area of the 38th Regiment in Qiemo County, located in the extremely arid region at the southeastern edge of the Tarim Basin. For the first time, irrigation experiments with different water qualities, ranging from 0.5 to 3.0 g/L, were conducted under varying groundwater depths for multiple crops. Through indoor soil column experiments and numerical simulations of water and salt in the unsaturated zone, the study reveals the water and salt migration patterns in the root zones of watermelon, corn, jujube, and peanuts. It was found that the process of soil water and salt transport exhibits significant differentiation characteristics in the vertical direction, with the surface layer responding most rapidly to changes in moisture and salinity, while the middle and deep layers show certain lag and buffering effects. The study also examined the spatiotemporal distribution trends of soil water and salt under different water quality and quantity irrigation conditions, drawing nonlinear threshold response curves for groundwater depth and determining the optimal groundwater depth under various irrigation conditions. The results indicate: (1) for the four crops under freshwater (0.5 g/L) irrigation and actual irrigation water conditions, soil salinity is safe at groundwater depths of 1–2 m; (2) under slightly saline water (2.0 g/L) irrigation, the safe groundwater depth (GWD) ranges for corn, peanuts, watermelon, and jujube root zones are 3.5–4.2 m, 1.2–3.5 m, ≥2.9 m, and ≥1.6 m, respectively, with crop sensitivity ranking as “corn > peanuts > watermelon > jujube”; and (3) under saline water (3.0 g/L) irrigation, the salinity tolerance thresholds for corn and peanuts root zones are exceeded regardless of shallow or deep groundwater depths, while the upper limits of salinity tolerance thresholds for watermelon and jujube correspond to groundwater depths of 2.9 m and 2.1 m, respectively, with increased groundwater depth making soil salinity increasingly safe. The study proposes a “sensitive-suitable-reinforced” three-zone paradigm and constructs a threshold table for optimal crop layout in arid areas based on the synergistic dual factors of “water quality–water quantity,” providing a theoretical basis for crop layout considering the spatial heterogeneity of groundwater occurrence. This has guiding value for arid oases in addressing the dual stress of water quality deterioration and salinization. Full article

Background: Traditionally, wild jujube (Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou) has been used to nourish the heart, calm the spirit, and arrest spontaneous sweating. Modern research confirms its broad pharmacological activities, including antioxidant, anti-inflammatory, neuroprotective, and cognitive-enhancing effects. This study aims to isolate and characterize the structure of jujube polysaccharides and evaluate their protective effects against oxidative stress damage in neural stem cells (NSCs). Methods: We successfully isolated and purified a novel pectin polysaccharide (ZJP-2) from wild jujube. Its structure was characterized in detail using high-performance liquid chromatography coupled with multi-angle laser light scattering and refractive index detection (HPLC-MALS-RI), high-performance anion exchange chromatography (HPAEC), gas chromatography–mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy. Results: Structural analysis revealed that ZJP-2 is a pectin heteropolysaccharide with a molecular weight of approximately 67.93 kDa. Its monosaccharide composition primarily includes galac-turonic acid (GalA), arabinose (Ara), rhamnose (Rha), galactose (Gal), and glucose (Glc). The backbone consists of α-GalA and rhamnose-galacturonic acid-I (RG-I) domains linked by (1→4)-glycosidic bonds. NMR spectroscopy further confirmed its glycosidic bond types. In activity assessment, our study demonstrated that ZJP-2 significantly alleviated DMNQ-induced oxidative stress damage in C17.2 neural stem cells. Its protective effect was achieved by reducing intracellular reactive oxygen species (ROS) levels and upregulating the mRNA expression of antioxidant genes associated with the signaling axis (p < 0.05). Moreover, ZJP-2 suppressed DMNQ-induced overexpression of Nestin and NeuN (p < 0.05), contributing to the maintenance of NSCs’ undifferentiated state and functional homeostasis. Conclusions: In conclusion, ZJP-2 possesses distinct structural characteristics and significant neuroprotective potential, supporting its development as a natural functional food or dietary supplement for preventing oxidative stress-related neural damage. Full article

Background/Objectives: Ziziphus jujuba var. spinosa (sour jujube) is a traditional medicinal plant with remarkable tolerance to abiotic stresses, particularly salinity. However, the regulatory mechanisms underlying its salt stress tolerance remain unclear. NHX genes play a crucial role in plant adaptation to salt stress by mediating Na⁺/K⁺ transport to maintain intracellular ion homeostasis and pH balance. Although the NHX gene family has been characterized in many plant species, its functional characteristics in sour jujube have not yet been systematically investigated. Methods: In this study, using Arabidopsis thaliana as a reference, we identified NHX genes in sour jujube through genome-wide analysis and molecular approaches, and systematically analyzed their phylogenetic relationships, chromosomal locations, conserved motifs, gene structures, cis-regulatory elements in promoter regions, and expression patterns under abiotic stresses, particularly salt stress. Results: The results revealed the presence of eight NHX genes distributed across six chromosomes in sour jujube, which were classified into three subfamilies: Vac-class, Endo-class, and PM-class. Members within the same evolutionary clade exhibited high structural conservation in motif composition and gene architecture. Except for the PM-class, all other clades contained amiloride-binding sites (FF(I/L)(Y/F)LFLLPPI). Analysis of cis-regulatory elements indicated that the promoter regions of these genes were enriched with elements related to defense responses, stress adaptation, and phytohormone signaling, further supporting their role in plant environmental adaptation. Additionally, the qRT-PCR analysis showed that most of the ZjNHX genes in both roots and leaves are up-regulated by salt. Notably, ZjNHX1 expression in roots increased approximately 40-fold within 3 h, whereas ZjNHX2 and ZjNHX3 were strongly induced in leaves under prolonged salt exposure. Conclusions: Taken together, this work gives a detailed overview of the ZjNHX genes and their important roles in response to salt stress in sour jujube. Our findings also provide a foundation for further functional characterization of this gene family. Full article