Search Results (23)

Activity patterns constitute a critical adaptive trait in large carnivores, enabling them to manage interspecific competition, enhance their foraging efficiency, and adapt to fluctuating environmental conditions. At the community level, elucidating the temporal activity allocation of sympatric large carnivores is essential for understanding species coexistence mechanisms. However, the activity patterns of most large carnivores remain inadequately explored. In this study, spanning a survey period from June 2014 to April 2024, we employed infrared camera technology to collect a total of 3312, 352, 240, and 79 independently validated photographs of snow leopards (Panthera uncia Schreber, 1775), wolves (Canis lupus Linnaeus, 1758), brown bears (Ursus arctos Linnaeus, 1758), and Eurasian lynx (Lynx lynx Linnaeus, 1758), respectively, across six distinct regions in the Sanjiangyuan Region (SR) and during different monitoring time periods. We utilized kernel density estimation and the coefficient of overlaps to assess diel activity pattern overlap and competitive intensities through pairwise comparisons among these four large carnivores. An analysis of the diel activity rhythm curves revealed that all four large carnivores predominantly exhibited nocturnal behavior, although their peak activity periods differed notably. Furthermore, the diel activity rhythm overlap between each pair of species showed moderate to high intensity throughout the year (0.5 ≤ Δ < 1), including during both the cold and warm seasons. Specifically, the diel activity rhythms of snow leopards and wolves, snow leopards and Eurasian lynx, and wolves and Eurasian lynx exhibited high levels of overlap annually and during the cold season (0.8 ≤ Δ < 1) but only moderate overlap during the warm season (0.5 ≤ Δ < 0.8). Our findings suggest that the diel activity rhythms of these four large carnivore species exhibited considerable overlap, potentially intensifying interspecific competition. This study advances our knowledge on the competitive and coexistence mechanisms of large carnivores in high-altitude mountainous ecosystems, offering critical data for their conservation and management. Full article

Globally, national governments have committed to restoring their native biodiversity, which can include the reintroduction of species. Amassing public support can prove difficult when the species is considered a contestable species, such as the Eurasian lynx, Lynx lynx, within the UK. Using an e-questionnaire widely distributed across social media platforms enabled the investigation of public perceptions regarding any proposed lynx reintroduction to England. The majority of the public generally supported the idea of a lynx reintroduction to England. Further investigation by societal groups allowed for greater and more detailed knowledge of perceptions, attitudes, and potential barriers to the potential reintroduction of this contestable species. There were varying opinions both negative and positive, between distinct societal groups, while respondent statements highlighted justifications for their positions. A closer investigation identified specific areas for educational efforts and engagement prior to any public consultation. Many negative opinions were based on either a lack of knowledge or misinformation, which highlighted where educational efforts should be targeted. Misinformation appeared rife within the farmer, vet, and wildlife photography groups. The study highlighted that applying both macro- and micro-scale analyses greatly benefits the identification, detail, and specific issues that need to be addressed, therefore enabling more efficient planning of relevant actions to address concerns before proceeding with such a proposal, especially at a time when funding is limited. Full article

The Eurasian lynx (Lynx lynx) is listed in CITES Appendix II and is protected under the Bern Convention and the EU Habitats Directive, yet it remains a frequent target of wildlife crime, highlighting the urgent need for reliable identification methods. This study focuses on determination and DNA quantification of the Lynx spp. using quantitative real-time PCR (qPCR). The Llynx Qplex quantification multiplex system effectively distinguishes Lynx spp. from other Feliformia species by targeting mitochondrial and nuclear markers. Additionally, we present the results of the developmental validation of the Llyn STRplex system for individual identification and databasing using six STR loci. This study followed ISFG recommendations for non-human DNA testing and developmental validation guidelines. Both systems demonstrate high sensitivity (5 pg genomic DNA for Llynx Qplex and 30 pg of mtDNA for Llyn STRplex) and high specificity to Lynx spp., confirmed by testing against 16 related Feliformia species. Robustness was evaluated, showing sensitivity to temperature variation, and both repeatability and reproducibility were successfully tested across replicates and conditions. Given that forensic casework often involves degraded and limited biological material, molecular tools must be both sensitive and specific to ensure accurate results. Developing precise and efficient tools is essential for supporting investigations of wildlife crime involving the Eurasian lynx, as well as efforts aimed at conserving the species. Full article

One of the main threats for the survival of the Iberian lynx is infectious disease. Feline parvoviruses cause often fatal diseases in cats and have been isolated from different species of Felidae and other carnivores. The present study is the first description of a parvoviral sequence isolated from the brain of an Iberian lynx which died four weeks after being transferred to a quarantine centre from a hunting estate in Castilla-La-Mancha (southern border of the Iberian plateau). Four days prior to death, it had developed anorexia and muscle weakness. The nucleotide sequence, at 4589 nt long (GenBank PP781551), was most proximal to that isolated from a Eurasian badger in Italy but also showed great homology with others from cats and other carnivores isolated in Spain and Italy, including that from a cat sequenced by us to elucidate the origin of the infection, which has not been clarified. The phylogenetic analysis of the capsid protein, VP2, which determines tropism and host range, confirmed that the lynx sequence was most proximal to feline than to canine parvoviruses, and was thus typed as Protoparvovirus carnivoran1. More studies, including serology, are needed to understand the pathogenesis of this infection. Full article

The Eurasian lynx (Lynx lynx), a widespread wild felid on the Eurasian continent, is currently classified as “critically endangered” in Germany. Understanding the impact of infectious agents is of particular importance for the continued conservation of these animals, especially regarding pathogens with broad host ranges and risk of interspecies transmission. Feline leukemia virus (FeLV) is known to infect wild and domestic felids worldwide, including several species of lynx, but it has not been reported thus far in the Eurasian lynx. In September 2020, a 16-month-old female Eurasian lynx from the Bavarian Forest, Germany, showed a sudden onset of gastrointestinal signs such as anorexia, diarrhea, and vomiting, and died within one week. Macroscopic and histologic examination revealed hemorrhagic-necrotizing enteritis and typhlocolitis, with the degeneration of crypts and crypt abscesses, as well as depleted Peyer’s patches. In addition, the animal showed lymphoid depletion (lymph nodes, thymus, and spleen) and hypocellularity of the bone marrow. FeLV infection was confirmed by immunohistochemistry and next generation sequencing. A secondary bacterial infection with hemolytic Escherichia coli and Clostridium perfringens type A was present in the intestine. This is the first report of FeLV-associated enteritis, lymphoid depletion and bone marrow suppression with associated secondary bacterial infection in a Eurasian lynx. Full article

Amid the SARS-CoV-2 pandemic, concerns surfaced regarding the spread of the virus to wildlife. Switzerland lacked data concerning the exposure of free-ranging animals to SARS-CoV-2 during this period. This study aimed to investigate the potential exposure of Swiss free-ranging wildlife to SARS-CoV-2. From 2020 to 2023, opportunistically collected samples from 712 shot or found dead wild mustelids (64 European stone and pine martens, 13 European badgers, 10 European polecats), canids (449 red foxes, 41 gray wolves, one golden jackal) and felids (56 Eurasian lynx, 18 European wildcats), as well as from 45 captured animals (39 Eurasian lynx, 6 European wildcats) were tested. A multi-step serological approach detecting antibodies to the spike protein receptor binding domain (RBD) and N-terminal S1 subunit followed by surrogate virus neutralization (sVNT) and pseudotype-based virus neutralization assays against different SARS-CoV-2 variants was performed. Additionally, viral RNA loads were quantified in lung tissues and in oronasal, oropharyngeal, and rectal swabs by reverse transcription polymerase chain reactions (RT-qPCRs). Serologically, SARS-CoV-2 exposure was confirmed in 14 free-ranging Swiss red foxes (prevalence 3.1%, 95% CI: 1.9–5.2%), two Eurasian lynx (2.2%, 95% CI: 0.6–7.7%), and one European wildcat (4.2%, 95% CI: 0.2–20.2%). Two positive foxes exhibited neutralization activity against the BA.2 and BA.1 Omicron variants. No active infection (viral RNA) was detected in any animal tested. This is the first report of SARS-CoV-2 antibodies in free-ranging red foxes, Eurasian lynx, and European wildcats worldwide. It confirms the spread of SARS-CoV-2 to free-ranging wildlife in Switzerland but does not provide evidence of reservoir formation. Our results underscore the susceptibility of wildlife populations to SARS-CoV-2 and the importance of understanding diseases in a One Health Concept. Full article

Apex predators were extirpated from the UK hundreds of years ago. Practitioners have recently advocated for reintroductions of predators in the UK given their role as keystone species. For a reintroduction to take place, a survey of public desirability needs to be conducted. We aimed to understand the perspectives of people across the United Kingdom on the reintroduction of Eurasian lynxes, gray wolves, and brown bears. We collected data via mixed-methods questionnaires from 78 key stakeholders, such as farmers, the general public, nature conservationists, and those in environmental sectors, with these areas being seen as the most affected by these reintroductions. It was organizations that front these stakeholder groups which mainly distributed the survey as well as social media groups. We ran a path analysis, which highlighted that there was a negative correlation between respondents viewing the species as a danger toward livestock and humans, and the respondent seeing it as being good to have that species in their region. Overall, participants who stated that rewilding their region was a priority also stated that it would be good to have Eurasian lynx in their region. That said, 70% of farmers strongly disagreed with the reintroduction of Eurasian lynx. These findings showed that much of the United Kingdom would welcome the reintroduction of Eurasian lynx, but both farmers and the public would fear the loss of livestock that may come along with these species. Support can be gained through education and knowledge, which is a necessary step needed especially targeting farmers before any reintroduction plans can be made. Full article

Trace element pollution can adversely affect the brains of individuals and thus impact the entire population of apex predators, such as large European carnivores. We assessed exposure to prominent neurotoxicants As, Cd, Hg and Pb by measuring their brain stem levels in brown bears (n = 114), grey wolves (n = 8), Eurasian lynx (n = 3), and golden jackals (n = 2) sampled in 2015–2022 in Croatia. The highest of the non-essential elements was the Pb level in the bearsʼ brains (median, Q1–Q3; 11.1, 7.13–24.1 μg/kg wet mass), with 4% of animals, all subadults, exceeding the established normal bovine levels (100 μg/kg wet mass). Species-specific differences were noted for Ca, Cd, Cu, Fe, Pb and Se brain levels. Female brown bears had higher As brain levels than males. Cubs and yearlings had lower brain Cd, but higher Zn, while subadults had higher Cu than adult bears. Hepatic As, Cd, Cu and Hg levels were shown to be a moderate proxy for estimating brain levels in bears (r_S = 0.30–0.69). Multiple associations of As, Cd, Hg and Pb with essential elements pointed to a possible interaction and disturbance of brain Ca, Cu, Fe, Se and Zn homeostasis. Non-essential element levels in the brains of four studied species were lower than reported earlier for terrestrial meso-carnivores and humans. The age and sex of animals were highlighted as essential factors in interpreting brain element levels in ecotoxicological studies of large carnivores. Full article

Knowledge concerning the relative importance of biotic and abiotic factors associated with the long-term settlement of large carnivores in human-dominated landscapes is important for effective species conservation. However, identifying the major drivers of large carnivore occurrences at a continental scale is challenging as several factors can act in concert to affect large carnivore distributions. Thus, we investigated the relationship between the permanent and sporadic occurrences of large carnivores (brown bear, Eurasian lynx, grey wolf and wolverine) and three groups of predictor variables (i.e., landscape attributes, human disturbance and prey availability) on the continental scale in Europe. Specifically, we used generalized linear models (GLMs) and variation partitioning to estimate the independent and cumulative effects of the three predictor groups on large carnivore occurrences. The explained variance for permanent vs. sporadic occurrence was highest for the wolverine (42.8% and 29.7%), followed by the brown bear (20.5% and 16.4%), Eurasian lynx (15.2% and 11.6%) and grey wolf (15.8% and 6.1%). Landscape attributes, such as forest cover, shrub-land cover, altitude and slope, were positively related to the occurrence of grey wolf, brown bear and Eurasian lynx, whereas human disturbance (human population density, distance to roads and to human settlements) was negatively related to wolverine occurrence. For all species, shared effects between landscape attributes and human disturbance accounted for a considerable portion of the explained variation in both permanent and sporadic occurrence, and landscape attributes were generally more important for explaining permanent than sporadic occurrence, except for the wolverine. Prey availability was marginally associated with the permanent occurrence of the grey wolf but we found no statistical effect of prey availability on the occurrence of the other large carnivores. In conclusion, the sporadic occurrence of large carnivores in Europe is more stochastic and less predictable than their permanent occurrence. Landscape attributes and their joint effects with human disturbance are the most important factors related to the recolonization dynamics of large carnivores, although wolverine distributions appear strongly limited by human disturbance itself. Domestic prey availability seems to play a subordinate role in driving the recent distribution dynamics of large carnivores at the continental scale. Thus, our results are relevant also in light of the ongoing recolonization of large carnivores which, in many rural areas, caused concerns among the locals, especially in relation to conflicts with human activities. For these reasons, we stress the need to continue and improve large carnivore monitoring across Europe. Full article

Wild carnivores are definitive hosts and potential reservoirs for the tapeworm Echinococcus sp. which can cause cystic and alveolar echinococcosis. Both are considered neglected and important food-borne pandemics. This study is the first to molecularly test Slovenian wild carnivores for Echinococcus species that can cause disease in humans. Fecal samples from 210 red foxes (Vulpes vulpes), 42 wolves (Canis lupus), 39 golden jackals (Canis aureus), 18 martens (Marten sp.), 2 Eurasian lynx (Lynx lynx), 2 European badger (Meles meles), and 1 Eurasian otter (Lutra lutra) were examined for Echinococcus granulosus sensu lato (EGsl: E. granulosus sensu stricto, E. canadensis) and E. multilocularis (EM) using real-time PCR. Red foxes (29.1%) and golden jackals (18%) were positive for EM. All animals examined were negative for EGsl. Univariate analysis showed no significant differences in EM prevalence with respect to animal species (red fox vs., golden jackal) (p = 0.22), age (p = 0.12), and sex (p = 0.18). Prevalence of EM was associated with the region (p < 0.001), with regions in central and southern Slovenia having higher EM prevalence and risk of infection. Due to the increase in population and expansion of habitat, the golden jackal may soon become as important definitive host for EM as the red fox. Full article

The Turkestan lynx (Lynx lynx isabellina Blyth, 1847) is a rare and understudied subspecies of the Eurasian lynx occupying the mountains of Central and South Asia. This elusive felid’s northwestern range includes the Tien Shan and Zhetisu Alatau mountains in the border region of Kazakhstan, China, Kyrgyzstan, and Uzbekistan. As the first step to conserve this vulnerable carnivore, we have conducted the first full-scale research from 2013 until 2022 on its distribution in this region. Using 132 environmental predictors of 359 lynx sightings, we have created species habitat distribution models across the lynx’s northwestern range using machine learning approaches (Maximum Entropy—MaxEnt). Additionally, we created species distribution forecasts based on seven bio-climatic environmental predictors with each three different future global climate model scenarios. To validate these forecasts, we have calculated the changes in the lynx distribution range for the year 2100, making the first species distribution forecast for the Turkestan lynx in the area. Additionally, it provides insight into the possible effects of global climate change on this lynx population. Based on these distribution models, the lynx population in the Northern and Western Tien Shan and Zhetisu Alatau plays a significant role in maintaining the stability of the whole subspecies in its northwestern and global range, while the distribution forecast shows that most lynx distribution ranges will reduce in all future climate scenarios, and we might face the Turkestan lynx’s significant distribution range decline under the ongoing and advancing climate change conditions. For a future (year 2100) warming scenario of 3 deg. C (GCM IPSL), we observe a decrease of 35% in Kazakhstan, 40% in Kyrgyzstan, and 30% in China as the three countries with the highest current predicted distribution range. For a milder temperature increase of 1.5–2 deg. C. (GCM MRI), we observe an increase of 17% Kazakhstan, decrease of 10% in Kyrgyzstan, and 57% in China. For a cooling scenario of approx. 1–1.5 deg. C (GCM MIROC), we observe a decrease of 14% Kazakhstan, increase of 11% in Kyrgyzstan, and a decrease of 13% in China. These modeled declines indicate the necessity to create new and expand the existing protected areas and establish ecological corridors between the countries in Central and South Asia. Full article

Automatic methods for detecting and delineating relief features allow remote and low-cost mapping, which has an outstanding potential for wildlife ecology and similar research. We applied a filled-DEM (digital elevation model) method using LiDAR (Light Detection and Ranging) data to automatically detect dolines and other karst depressions in a rugged terrain of the Dinaric Mountains, Slovenia. Using this approach, we detected 9711 karst depressions in a 137 km² study area and provided their basic morphometric characteristics, such as perimeter length, area, diameter, depth, and slope. We performed visual validation based on shaded relief, which indicated 83.5% accordance in detecting depressions. Although the method has some drawbacks, it proved suitable for detection, general spatial analysis, and calculation of morphometric characteristics of depressions over a large scale in remote and forested areas. To demonstrate its applicability for wildlife research, we applied it in a preliminary study in combination with GPS-telemetry data to assess the selection of these features by two wild felids, the Eurasian lynx (Lynx lynx) and the European wildcat (Felis silvestris). Both species selected for vicinity of karst depressions, among which they selected for larger karst depressions. Lynx also regularly killed ungulate prey near these features, as we found more than half of lynx prey remains inside or in close vicinity of karst depressions. These results illustrate that karstic features could play an important role in the ecology of wild felids and warrant further research, which could be considerably assisted with the use of remote detection of relief features. Full article

This paper presents the evaluation of 36 convolutional neural network (CNN) models, which were trained on the same dataset (ImageNet). The aim of this research was to evaluate the performance of pre-trained models on the binary classification of images in a “real-world” application. The classification of wildlife images was the use case, in particular, those of the Eurasian lynx (lat. “Lynx lynx”), which were collected by camera traps in various locations in Croatia. The collected images varied greatly in terms of image quality, while the dataset itself was highly imbalanced in terms of the percentage of images that depicted lynxes. Full article

Teaching in a formal learning environment mainly focuses on gaining knowledge, and scarcely on the development of pro-environmental attitudes. Knowledge can also be gained in informal learning institutions, such as zoos, and their potential use in general public education should not be neglected. This paper explores factors influencing the conservation beliefs of zoo visitors about brown bears, grey wolves, and Eurasian lynx. The study undertaken in Zoo Ljubljana (Slovenia) consisted of surveys performed in 2009 (n = 613) and in 2021 (n = 257). The levels of knowledge and education influenced both supporting and opposing beliefs about the three large carnivore species. The gender factor was less uniform: both supporting and opposing beliefs about lynx were demonstrated, but only opposing beliefs about brown bear and wolf. The study indicates that knowledge has the most significant influence on conservation beliefs, thus highlighting the importance of educational and communication activities in management and conservation actions regarding large carnivore species. The varied gender influence suggests that species-specific educational activities should be encouraged. Full article

The Eurasian lynx (Lynx lynx) represents an endangered wild felid species. In Germany, it currently occurs in three isolated populations in and around the Harz Mountains, the Palatinate Forest and the Bavarian Forest. Lynx parasitic infections affect animal health and might have an influence on population performance. Therefore, we investigated the protozoan and helminth fauna of free-ranging Eurasian lynx of the Harz population with emphasis on zoonotic parasites. Individual scat samples (n = 24) were collected from wild animals between 2019 and 2021 in the Harz National Park and surrounding areas. In total, 15 taxa of endoparasites were detected, including seven nematodes (i.e., Aelurostrongylus abstrusus, Angiostrongylus spp., Uncinaria stenocephala, Toxascaris leonina, Toxocara cati, Cylicospirura spp. and Capillaria spp.), one cestode (Diphyllobothriidae) and one trematode (Heterophylidae) as well as six protozoans (i.e., Cystoisospora rivolta, Cystoisospora felis, Toxoplasma gondii/Hammondia spp., Sarcocystis spp., Giardia intestinalis and Cryptosporidium spp.). Moreover, first-stage larvae (L1) of spurious lungworm, Protostrongylus pulmonalis, originating from lagomorph preys were identified. This work represents the first report on patent A. abstrusus and Angiostrongylus spp. infections in wild German Eurasian lynxes. Some of the identified parasites represent relevant pathogens for lynxes, circulating between these carnivorous definitive hosts and a variety of mammalian and invertebrate intermediate hosts, e.g., Sarcocystis spp., T. gondii/Hammondia spp., T. cati, T. leonina, A. abstrusus and Angiostrongylus spp., while others are considered exclusively pathogenic for wild felids (e.g., Cylicospirura spp., C. rivolta, C. felis). This study provides insights in the occurrence of zooanthroponotically relevant metazoan (i.e., T. cati and U. stenocephala) and protozoan (i.e., G. intestinalis) species in free-ranging lynx. The present work should be considered as a baseline study for future monitoring surveys on endoparasites circulating in wild Eurasian lynx for appropriate management practices in lynx conservation strategies in Europe. Full article